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https://www.readbyqxmd.com/read/28743003/patterned-disordered-cell-motion-ensures-vertebral-column-symmetry
#1
Dipjyoti Das, Veena Chatti, Thierry Emonet, Scott A Holley
The biomechanics of posterior embryonic growth must be dynamically regulated to ensure bilateral symmetry of the spinal column. Throughout vertebrate trunk elongation, motile mesodermal progenitors undergo an order-to-disorder transition via an epithelial-to-mesenchymal transition and sort symmetrically into the left and right paraxial mesoderm. We combine theoretical modeling of cell migration in a tail-bud-like geometry with experimental data analysis to assess the importance of ordered and disordered cell motion...
July 24, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28742733/intravoxel-incoherent-motion-model-free-determination-of-tissue-type-in-abdominal-organs-using-machine-learning
#2
Alexander Ciritsis, Cristina Rossi, Moritz C Wurnig, Valerie Phi Van, Andreas Boss
PURPOSE: For diffusion data sets including low and high b-values, the intravoxel incoherent motion model is commonly applied to characterize tissue. The aim of the present study was to show that machine learning allows a model-free approach to determine tissue type without a priori assumptions on the underlying physiology. MATERIALS AND METHODS: In 8 healthy volunteers, diffusion data sets were acquired using an echo-planar imaging sequence with 16 b-values in the range between 0 and 1000 s/mm...
July 24, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28742277/the-immediate-and-short-term-effects-of-real-time-knee-adduction-moment-feedback-on-the-peak-and-cumulative-knee-load-during-walking
#3
Brennan Jackson, Keith E Gordon, Alison H Chang
The peak external knee adduction moment (pKAM), KAM impulse, and peak knee flexion moment (pKFM) during gait are important loading variables in medial tibiofemoral osteoarthritis. We evaluated the effects of gait modification, using real-time pKAM visual feedback, on pKAM, KAM impulse, and pKFM; and whether participants could maintain the KAM-reducing gait after feedback removal. Eleven healthy individuals performed a series of walking trials on a split-belt instrumented treadmill under 4 conditions of Baseline, Feedback, No Feedback Early, and No Feedback Late...
July 25, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28742107/analyzing-the-locomotory-gaitprint-of-caenorhabditis-elegans-on-the-basis-of-empirical-mode-decomposition
#4
Li-Chun Lin, Han-Sheng Chuang
The locomotory gait analysis of the microswimmer, Caenorhabditis elegans, is a commonly adopted approach for strain recognition and examination of phenotypic defects. Gait is also a visible behavioral expression of worms under external stimuli. This study developed an adaptive data analysis method based on empirical mode decomposition (EMD) to reveal the biological cues behind intricate motion. The method was used to classify the strains of worms according to their gaitprints (i.e., phenotypic traits of locomotion)...
2017: PloS One
https://www.readbyqxmd.com/read/28742079/the-dcdc2-deletion-is-not-a-risk-factor-for-dyslexia
#5
T S Scerri, E Macpherson, A Martinelli, W C Wa, A P Monaco, J Stein, M Zheng, C Suk-Han Ho, C McBride, M Snowling, C Hulme, M E Hayiou-Thomas, M M Y Waye, J B Talcott, S Paracchini
Dyslexia is a specific impairment in learning to read and has strong heritability. An intronic deletion within the DCDC2 gene, with ~8% frequency in European populations, is increasingly used as a marker for dyslexia in neuroimaging and behavioral studies. At a mechanistic level, this deletion has been proposed to influence sensory processing capacity, and in particular sensitivity to visual coherent motion. Our re-assessment of the literature, however, did not reveal strong support for a role of this specific deletion in dyslexia...
July 25, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28741973/characteristics-of-functional-impairment-in-patients-with-long-standing-rheumatoid-arthritis-based-on-range-of-motion-of-joints-baseline-data-from-a-multicenter-prospective-observational-cohort-study-to-evaluate-the-effectiveness-of-joint-surgery-in-the-treat
#6
Toshihisa Kojima, Hajime Ishikawa, Sakae Tanaka, Nobuhiko Haga, Keiichiro Nishida, Masao Yukioka, Jun Hashimoto, Hisaaki Miyahara, Yasuo Niki, Tomoatsu Kimura, Hiromi Oda, Shuji Asai, Koji Funahashi, Masayo Kojima, Naoki Ishiguro
OBJECTIVE: To explore the characteristics of functional impairment in patients with established rheumatoid arthritis (RA) based on the range of motion (ROM) of joints in a prospective observational study of RA patients undergoing joint surgery. METHODS: We collected data on demographics, Health Assessment Questionnaire Disability Index (HAQ-DI), and the ROM of large joints including the shoulder, elbow, wrist, hip, knee, and ankle. Associations between the ROM of each joint and disability in the eight HAQ-DI categories were determined using receiver operating characteristic (ROC) and logistic regression analyses...
July 25, 2017: Modern Rheumatology
https://www.readbyqxmd.com/read/28741927/rapid-antibiotic-susceptibility-testing-of-uropathogenic-e-coli-by-tracking-sub-micron-scale-motion-of-single-bacterial-cells
#7
Karan Syal, Simon Shen, Yunze Yang, Shaopeng Wang, Shelley E Haydel, Nongjian Tao
To combat antibiotic resistance, a rapid antibiotic susceptibility testing (AST) technology that can identify resistant infections at disease onset is required. Current clinical AST technologies take 1-3 days, which is often too slow for accurate treatment. Here we demonstrate a rapid AST method by tracking sub-µm scale bacterial motion with an optical imaging and tracking technique. We apply the method to clinically relevant bacterial pathogens, Escherichia coli O157: H7 and uropathogenic E. coli (UPEC) loosely tethered to a glass surface...
July 25, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28741732/lung-cancer-short-term-reproducibility-of-intravoxel-incoherent-motion-parameters-and-apparent-diffusion-coefficient-at-3t
#8
Jianqin Jiang, Jianbin Yin, Lei Cui, Xiaowen Gu, Rongfang Cai, Shenchu Gong, Yiming Xu, Hang Ma, Jian Mao
PURPOSE: To prospectively evaluate the short-term reproducibility of intravoxel incoherent motion (IVIM) parameters and apparent diffusion coefficient (ADC) in lung cancer patients. MATERIALS AND METHODS: In all, 50 patients (50 lesions) underwent free-breathing diffusion-weighted imaging (DWI) (b = 0, 300, 800 s/mm(2) ) and IVIM (10 b-values, 0-1000 s/mm(2) ) scans twice (0.5-1-hour interval) at 3T. Regions of interests (ROIs) were drawn on ADC maps and IVIM images to derive the mean ADC value and IVIM parameters D, D*, and f...
July 25, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28741721/left-ventricular-septal-hypertrophy-in-elderly-patients-with-aortic-stenosis
#9
Minako Katayama, Prasad M Panse, Christopher B Kendall, John R Daniels, Stephen S Cha, F David Fortuin, John P Sweeney, Patrick A DeValeria, Louis A Lanza, Marek Belohlavek, Hari P Chaliki
OBJECTIVES: Left ventricular (LV) septal hypertrophy in aortic stenosis raises diagnostic and therapeutic questions. However, the etiology and clinical consequences of this finding have not been well studied. The aim of this study was to perform a morphologic evaluation of the LV in aortic stenosis and to investigate the contributing factors and consequences of septal hypertrophy. METHODS: Patients with moderate or severe aortic stenosis were prospectively enrolled...
July 25, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28741366/comparison-of-bi-exponential-and-mono-exponential-models-of-diffusion-weighted-imaging-for-detecting-active-sacroiliitis-in-ankylosing-spondylitis
#10
Haitao Sun, Kai Liu, Hao Liu, Zongfei Ji, Yan Yan, Lindi Jiang, Jianjun Zhou
Background There has been a growing need for a sensitive and effective imaging method for the differentiation of the activity of ankylosing spondylitis (AS). Purpose To compare the performances of intravoxel incoherent motion (IVIM)-derived parameters and the apparent diffusion coefficient (ADC) for distinguishing AS-activity. Material and Methods One hundred patients with AS were divided into active (n = 51) and non-active groups (n = 49) and 21 healthy volunteers were included as control. The ADC, diffusion coefficient ( D), pseudodiffusion coefficient ( D*), and perfusion fraction ( f) were calculated for all groups...
January 1, 2017: Acta Radiologica
https://www.readbyqxmd.com/read/28740983/combining-epr-spectroscopy-and-x-ray-crystallography-to-elucidate-the-structure-and-dynamics-of-conformationally-constrained-spin-labels-in-t4-lysozyme-single-crystals
#11
Philipp Consentius, Ulrich Gohlke, Bernhard Loll, Claudia Alings, Udo Heinemann, Markus C Wahl, Thomas Risse
Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is used to investigate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals. Within a single crystal, the oriented ensemble of spin bearing moieties results in a strong angle dependence of the EPR spectra. A quantitative description of the EPR spectra requires the determination of the unit cell orientation with respect to the sample tube and the orientation of the spin bearing moieties within the crystal lattice...
July 25, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28739084/the-repeatability-and-characteristics-of-right-ventricular-longitudinal-strain-imaging-by-speckle-tracking-echocardiography-in-healthy-dogs
#12
T Morita, K Nakamura, T Osuga, N Yokoyama, N Khoirun, K Morishita, N Sasaki, H Ohta, M Takiguchi
INTRODUCTION: To assess the repeatability and characteristics of echocardiographic indices of the right ventricular (RV) function derived from speckle-tracking echocardiography. ANIMALS: Fourteen laboratory Beagles and 103 privately owned dogs without cardiac disease were involved in this study. MATERIALS AND METHODS: Right ventricular longitudinal strain, strain rate, and a strain-related index for assessing RV dyssynchrony derived from speckle-tracking echocardiography were obtained by two different observers using five Beagles...
July 21, 2017: Journal of Veterinary Cardiology: the Official Journal of the European Society of Veterinary Cardiology
https://www.readbyqxmd.com/read/28738486/-association-between-collateral-circulation-and-myocardial-viability-evaluated-by-cardiac-magnetic-resonance-imaging-in-patients-with-coronary-artery-chronic-total-occlusion
#13
J N Li, L J Zhang, Y He, Y L Chen, R C Huang, S Z Lyu, X T Song
Objective: Late gadolinium enhancement(LGE) cardiac magnetic resonance imaging(CMR) was used to evaluate the myocardial viability of chronic total occlusion(CTO) in patients with coronary heart disease and to observe the relationship between collateral circulation and myocardium viability in these patients. Methods: This retrospective study included 40 patients with CTO diagnosed by invasive coronary angiography (CAG) from September 2015 to June 2016 in our department, all patients performed CMR examination within one week after CAG...
July 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28737407/label-free-monitoring-of-single-molecule-immunoreaction-with-a-nanopipette
#14
Ru-Jia Yu, Yi-Lun Ying, Yong-Xu Hu, Rui Gao, Yi-Tao Long
The nanopipette has been employed for the single molecule analysis due to its advantage of easy fabrication and controllable diameter. Herein, we present that the single molecule immunoreaction could be monitored by using the quartz nanopipette through the discrimination of characteristic blockade current, which reflect the intrinsic character of the individual unlabeled protein molecules due to its heterogenous motion in solution. Our methods show the ability to monitor the immunoreaction between single α-fetal protein (AFP) and its specific antibody in aqueous solution without any labelling...
July 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28736982/understanding-the-influence-of-flow-velocity-wall-motion-filter-pulse-repetition-frequency-and-aliasing-on-power-doppler-image-quantification
#15
Maricy R Martins, Wellington P Martins, Carlos A M Soares, Andre H Miyague, Marek J Kudla, Theo Z Pavan
OBJECTIVES: Although power Doppler imaging has been used to quantify tissue and organ vascularity, many studies showed that limitations in defining adequate ultrasound machine settings and attenuation make such measurements complex to be achieved. However, most of these studies were conducted by using the output of proprietary software, such as Virtual Organ computer-aided analysis (GE Healthcare, Kretz, Zipf, Austria); therefore, many conclusions may not be generalizable because of unknown settings and parameters used by the software...
July 24, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28736698/impact-of-automated-ica-based-denoising-of-fmri-data-in-acute-stroke-patients
#16
D Carone, R Licenik, S Suri, L Griffanti, N Filippini, J Kennedy
Different strategies have been developed using Independent Component Analysis (ICA) to automatically de-noise fMRI data, either focusing on removing only certain components (e.g. motion-ICA-AROMA, Pruim et al., 2015a) or using more complex classifiers to remove multiple types of noise components (e.g. FIX, Salimi-Khorshidi et al., 2014 Griffanti et al., 2014). However, denoising data obtained in an acute setting might prove challenging: the presence of multiple noise sources may not allow focused strategies to clean the data enough and the heterogeneity in the data may be so great to critically undermine complex approaches...
2017: NeuroImage: Clinical
https://www.readbyqxmd.com/read/28736356/finite-element-analysis-of-influence-of-axial-position-of-center-of-rotation-of-a-cervical-total-disc-replacement-on-biomechanical-parameters-simulated-two-level-replacement-based-on-a-validated-model
#17
Yang Li, Zhenjun Zhang, Zhenhua Liao, Zhongjun Mo, Weiqiang Liu
Finite element (FE) models have been widely used to predict biomechanical parameters of the cervical spine. Previous studies investigated the influence of position of rotational centers (RCs) of prostheses on the cervical biomechanical parameters after one-level total disc replacement (TDR). The purpose of this study was to make a further step by exploring the effects of axial position of RCs of prostheses on the cervical biomechanics after 2-level TDR. A validated FE model of C3-C7 segments and 2 prostheses, including the RC located at the superior endplate (SE) and inferior endplate (IE), was developed...
July 20, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28735949/hip-flexor-muscle-dysfunction-during-walking-at-self-selected-and-fast-speed-in-patients-with-aortoiliac-peripheral-arterial-disease
#18
Takaaki Kakihana, Osamu Ito, Yusuke Sekiguchi, Daisuke Ito, Hitoshi Goto, Daijirou Akamatsu, Yasuharu Matsumoto, Masahiro Kohzuki
OBJECTIVE: Intermittent claudication aggravates physical function and is associated with an increased risk of death in patients with peripheral arterial disease (PAD). Previous studies on kinetic parameters (joint moment and power) of lower limbs in these patients have largely focused on the decline in the ankle plantar flexor moment and power at self-selected (SS) walking speed, which may not be an optimal condition to induce claudication pain. In the present study, we investigated the abnormalities in joint kinetic parameters in patients with PAD at both SS and at fast walking speeds...
August 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28735876/methods-for-automatic-detection-of-artifacts-in-microelectrode-recordings
#19
Eduard Bakštein, Tomáš Sieger, Jiří Wild, Daniel Nova'k, Jakub Schneider, Pavel Vostatek, Dušan Urgošík, Robert Jech
BACKGROUND: Extracellular microelectrode recording (MER) is a prominent technique for studies of extracellular single-unit neuronal activity. In order to achieve robust results in more complex analysis pipelines, it is necessary to have high quality input data with a low amount of artifacts. We show that noise (mainly electromagnetic interference and motion artifacts) may affect more than 25% of the recording length in a clinical MER database. NEW METHOD: We present several methods for automatic detection of noise in MER signals, based on i) unsupervised detection of stationary segments, ii) large peaks in the power spectral density, and iii) a classifier based on multiple time- and frequency-domain features...
July 20, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28735170/effects-of-different-solvents-on-the-conformations-of-apoptotic-cytochrome-c-structural-insights-from-molecular-dynamics-simulation
#20
Gurusamy Muneeswaran, Subramanian Kartheeswaran, Kaliappan Muthukumar, Christopher D Dharmaraj, Chandran Karunakaran
Cytochrome c (cyt-c) upon binding with cardiolipin acquires peroxidase activity and is strictly connected to the pathogenesis of many human diseases including neurodegenerative and cardiovascular diseases. Interaction of cyt-c with cardiolipin mimics partial unfolding/conformational changes of cyt-c in different solvent environments. Dynamic pictures of these conformational changes of cyt-c are crucial in understanding their physiological roles in mitochondrial functions. Therefore, atomistic molecular dynamics (MD) simulations have been carried out to investigate the effect of different solvents (water, urea/water, MeOH and DMSO) on the structure and conformations of apoptotic cyt-c (Fe(3+))...
July 14, 2017: Journal of Molecular Graphics & Modelling
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