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Lucía Barbier-Torres, Paula Iruzubieta, David Fernández-Ramos, Teresa C Delgado, Daniel Taibo, Virginia Guitiérrez-de-Juan, Marta Varela-Rey, Mikel Azkargorta, Nicolas Navasa, Pablo Fernández-Tussy, Imanol Zubiete-Franco, Jorge Simon, Fernando Lopitz-Otsoa, Sofia Lachiondo-Ortega, Javier Crespo, Steven Masson, Misti Vanette McCain, Erica Villa, Helen Reeves, Felix Elortza, Maria Isabel Lucena, Maria Isabel Hernández-Alvarez, Antonio Zorzano, Raúl J Andrade, Shelly C Lu, José M Mato, Juan Anguita, Mercedes Rincón, María Luz Martínez-Chantar
Acetaminophen (APAP) is the active component of many medications used to treat pain and fever worldwide. Its overuse provokes liver injury and it is the second most common cause of liver failure. Mitochondrial dysfunction contributes to APAP-induced liver injury but the mechanism by which APAP causes hepatocyte toxicity is not completely understood. Therefore, we lack efficient therapeutic strategies to treat this pathology. Here we show that APAP interferes with the formation of mitochondrial respiratory supercomplexes via the mitochondrial negative regulator MCJ, and leads to decreased production of ATP and increased generation of ROS...
December 12, 2017: Nature Communications
Manpreet Singh, Natasha Gautam, Manpreet Kaur, Zoramthara Zadeng
A 20-year-old girl presented with a large pigmented lesion over the temporal aspect of left lateral canthus involving the lateral one-third of both eyelids, since birth. A slow increase in its size had been noticed for the past 2 years. On examination, a hairy nevus measuring 34 mm × 22 mm was noticed involving left eyelid skin, the mucocutaneous junction (MCJ), palpebral conjunctiva, and lateral canthus. The surface was irregularly thick and covered with long, thick, and pigmented hairs. A clinical diagnosis of kissing nevus of eyelids was kept and a surgical resection with reconstruction planned...
November 2017: Indian Journal of Ophthalmology
Manisha Chandwani, Rakesh Mittal, Shweta Chandak, Jitesh Pimpale
BACKGROUND: The purpose of this study was to evaluate the microbial reduction in deciduous molars using Morinda citrifolia juice (MCJ) as irrigating solution. MATERIALS AND METHODS: This was a randomized comparative study including 60 deciduous molars chosen among the patients belonging to the age group of 6-9 years based on the inclusion or exclusion criteria. The selected teeth were divided randomly into two groups based on irrigation solution used, that was, Group I (1% NaOCl) and Group II (MCJ)...
July 2017: Dental Research Journal
Suparna Ganguly Saha, Vini Sharma, Anuj Bharadwaj, Poorvi Shrivastava, Mainak Kanti Saha, Sandeep Dubey, Shubham Kala, Shubhra Gupta
INTRODUCTION: Irrigating solutions used for the elimination of micro-organisms during root canal preparation may affect the chemical and the physical properties of dentin thereby rendering the tooth more susceptible to fracture. Therefore careful and judicious selection of irrigant is required which have maximum benefits with minimum undesirable properties. AIM: The aim of the present study was to evaluate the effect of various endodontic irrigants on the micro-hardness of the root canal dentin...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
Haluk Çabuk, Fatmagül Kuşku Çabuk, Ali Çağrı Tekin, Süleyman Semih Dedeoğlu, Murat Çakar, Cem Dinçay Büyükkurt
PURPOSE: Impaired proprioception accuracy of the knee has been proposed as a local factor in the onset and progression of knee osteoarthritis. Patients with decreased numbers of mechanoreceptors could be more likely to develop arthrosis due to a loss in proprioception of the joint. We aimed to identify and quantify the mechanoreceptors of the posterior cruciate ligament (PCL), the anterior capsule (AC) and the medial meniscocapsular junction (MCJ) in knee arthrosis. METHODS: PCLs, ACs and MCJs were harvested from 30 patients with Kellgren and Lawrence grades 3 and 4 osteoarthritis (OA), and ten knees taken from five cadavers without OA were used as a control group...
June 23, 2016: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
Maria J Fernández-Cabezudo, Issam Faour, Kenneth Jones, Devin P Champagne, Mohammed A Jaloudi, Yassir A Mohamed, Ghada Bashir, Saeeda Almarzooqi, Alia Albawardi, M Jawad Hashim, Thomas S Roberts, Haytham El-Salhat, Hakam El-Taji, Adnan Kassis, Dylan E O'Sullivan, Brock C Christensen, James DeGregori, Basel K Al-Ramadi, Mercedes Rincon
Despite major advances in early detection and prognosis, chemotherapy resistance is a major hurdle in the battle against breast cancer. Identifying predictive markers and understanding the mechanisms are key steps to overcoming chemoresistance. Methylation-controlled J protein (MCJ, also known as DNAJC15) is a negative regulator of mitochondrial respiration and has been associated with chemotherapeutic drug sensitivity in cancer cell lines. Here we show, in a retrospective study of a large cohort of breast cancer patients, that low MCJ expression in breast tumors predicts high risk of relapse in patients treated with chemotherapy; however, MCJ expression does not correlate with response to endocrine therapy...
May 19, 2016: JCI Insight
Stephanie M Cox, David A Berntsen, Neil Chatterjee, Sheila B Hickson-Curran, Lyndon W Jones, Amir M Moezzi, Philip B Morgan, Jason J Nichols
PURPOSE: To describe the lid margin characteristics of contact lens wearers and relate them to comfort during lens wear. METHODS: Three study sites enrolled habitual contact lens wearers. Subjects completed the Comfort domain of the Contact Lens User Experience (CLUE) questionnaire, and each eye was graded for the presence of mucocutaneous junction (MCJ) displacement, lid margin irregularity, and lid margin vascularity. Examiners counted the number of meibomian gland (MG) orifices in the central centimeter of the lower eyelid and the number of those that showed pouting/plugging and vascular invasion...
August 2016: Optometry and Vision Science: Official Publication of the American Academy of Optometry
Devin P Champagne, Ketki M Hatle, Karen A Fortner, Angelo D'Alessandro, Tina M Thornton, Rui Yang, Daniel Torralba, Julen Tomás-Cortázar, Yong Woong Jun, Kyo Han Ahn, Kirk C Hansen, Laura Haynes, Juan Anguita, Mercedes Rincon
Mitochondrial respiration is regulated in CD8(+) T cells during the transition from naive to effector and memory cells, but mechanisms controlling this process have not been defined. Here we show that MCJ (methylation-controlled J protein) acted as an endogenous break for mitochondrial respiration in CD8(+) T cells by interfering with the formation of electron transport chain respiratory supercomplexes. Metabolic profiling revealed enhanced mitochondrial metabolism in MCJ-deficient CD8(+) T cells. Increased oxidative phosphorylation and subcellular ATP accumulation caused by MCJ deficiency selectively increased the secretion, but not expression, of interferon-γ...
June 21, 2016: Immunity
Gurkan Rasit Bayar, Shiuhyang Kuo, Cynthia L Marcelo, Stephen E Feinberg
PURPOSE: We present a straightforward and reproducible technique to create, in vitro, a construct containing a mucocutaneous junction (MCJ) with a transitional zone (vermilion) for fabrication of a microvascular prelaminated flap for use in lip reconstruction. MATERIALS AND METHODS: Cultured primary human skin keratinocytes and oral mucosal epithelial cells at premixed ratios of 50% skin cells to 50% oral cells, 25% skin cells to 75% oral cells, and 75% skin cells to 25% oral cells were grown on an AlloDerm dermal equivalent (LifeCell, Branchburg, NJ) to create an MCJ equivalent with a lip or transitional zone (vermilion) using a novel 3-dimensional (3D) culture device with a barrier to separate co-cultured skin and oral cells...
November 2016: Journal of Oral and Maxillofacial Surgery
Rajesh Podar, Gaurav P Kulkarni, Shifali S Dadu, Shraddha Singh, Shishir H Singh
OBJECTIVE: To evaluate and compare the antimicrobial efficacy of 6% Morinda citrifolia, Azadirachta indica, and 3% sodium hypochlorite (NaOCl) as root canal irrigants. MATERIALS AND METHODS: Thirty nonvital maxillary anteriors were randomly assigned to one of the three groups corresponding to the irrigant to be tested; 6% Morinda citrifolia juice (MCJ) (n = 10), A. indica (n = 10) and 3% NaOCl (n = 10). After the root canal access opening a root canal culture sample was taken with two paper points and cultured under aerobic and anaerobic conditions...
October 2015: European Journal of Dentistry
Jinzhong Lin, Yexia Zou, Kunlin Cao, Chengjie Ma, Zhengjun Chen
Two heme-dependent catalase genes were amplified from genomic DNA of Lactobacillus plantarum WCFS1 (KatE1) and Lactobacillus brevis ATCC 367 (KatE2), respectively, and a manganese-containing superoxide dismutase from Lactobacillus casei MCJΔ1 (MnSOD) were cloned into plasmid pELX1, yielding pELX1-KatE1, pELX1-KatE2 and pELX1-MnSOD, then the recombinant plasmids were transferred into L. casei MCJΔ1. The strains of L. casei MCJΔ1/pELX1-KatE1 and L. casei MCJΔ1/pELX1-KatE2 were tolerant at 2 mM H2O2. The survival rates of L...
May 2016: Journal of Industrial Microbiology & Biotechnology
Michael E Graham, Avanthi Chikka, Vijay K Goel
First metatarsocuneiform joint arthrodesis with a locking bone plate and screw system has been effectively used to correct metatarsus primus varus and instability of the first ray. The goal of the present cadaveric biomechanical study was to quantify and compare the inherent strength of the first metatarsocuneiform joint and surrounding bones fixated with the osteo-WEDGE(™) bone plate locking system (OW) with that of intact specimens. Fourteen fresh-frozen adult human cadaveric foot specimens consisting of the first metatarsal and medial cuneiform bones with intact joint capsules and ligaments were used...
May 2016: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
Nicolás Navasa, Itziar Martin-Ruiz, Estíbaliz Atondo, James D Sutherland, Miguel Angel Pascual-Itoiz, Ana Carreras-González, Hooman Izadi, Julen Tomás-Cortázar, Furkan Ayaz, Natalia Martin-Martin, Iviana M Torres, Rosa Barrio, Arkaitz Carracedo, Elias R Olivera, Mercedes Rincón, Juan Anguita
MCJ (DNAJC15) is a mitochondrial protein that regulates the mitochondrial metabolic status of macrophages and their response to inflammatory stimuli. CpG island methylation in cancer cells constitutes the only mechanism identified for the regulation of MCJ gene expression. However, whether DNA methylation or transcriptional regulation mechanisms are involved in the physiological control of this gene expression in non-tumor cells remains unknown. We now demonstrate a mechanism of regulation of MCJ expression that is independent of DNA methylation...
September 30, 2015: Scientific Reports
Seok Hoon Hong, Yong-Chan Kwon, Rey W Martin, Benjamin J Des Soye, Alexandra M de Paz, Kirsten N Swonger, Ioanna Ntai, Neil L Kelleher, Michael C Jewett
Site-specific incorporation of non-standard amino acids (NSAAs) into proteins opens the way to novel biological insights and applications in biotechnology. Here, we describe the development of a high yielding cell-free protein synthesis (CFPS) platform for NSAA incorporation from crude extracts of genomically recoded Escherichia coli lacking release factor 1. We used genome engineering to construct synthetic organisms that, upon cell lysis, lead to improved extract performance. We targeted five potential negative effectors to be disabled: the nuclease genes rna, rnb, csdA, mazF, and endA...
March 23, 2015: Chembiochem: a European Journal of Chemical Biology
Anusree Das, Jojo Kottoor, Joy Mathew, Sanjana Kumar, Saira George
AIM: To compare the changes in microhardness of root dentin caused by two novel irrigation regimens with conventional irrigation. MATERIALS AND METHODS: Forty extracted human permanent incisor teeth were selected. Decoronated roots were separated longitudinally to get 80 specimens that were embedded in autopolymerizing acrylic resin and grounded flat with silicon carbide abrasive papers. Of these, 60 root segments without any cracks or defects were selected and divided into four groups according to the irrigation regimen used (n = 15)...
November 2014: Journal of Conservative Dentistry: JCD
Mickey Plymale, Glenn S Fleisig, Stephen M Kocaj, William P Cooney, Timothy J Evans, E Lyle Cain, Jeffrey R Dugas
Meniscal injuries commonly occur concomitantly with anterior cruciate ligament (ACL) injuries. Although many types of meniscal injuries have been described in the literature, there has not been much focus on meniscal capsular junction (MCJ) tears. This lack of attention is concerning given that, in a survey of 67 orthopedic surgeons, 88% indicated that MCJ tears could be a source of chronic pain. In addition, we reviewed 781 ACL reconstructions at our clinic and found a 12.3% incidence of MCJ tear with primary ACL injury and a 23...
November 2014: American Journal of Orthopedics
Nicolás Navasa, Itziar Martín, Juan Manuel Iglesias-Pedraz, Naiara Beraza, Estíbaliz Atondo, Hooman Izadi, Furkan Ayaz, Sara Fernández-Álvarez, Ketki Hatle, Abhigyam Som, Oliver Dienz, Barbara A Osborne, Maria Luz Martínez-Chantar, Mercedes Rincón, Juan Anguita
Mitochondria contribute to macrophage immune function through the generation of reactive oxygen species, a byproduct of the mitochondrial respiratory chain. MCJ (also known as DnaJC15) is a mitochondrial inner membrane protein identified as an endogenous inhibitor of respiratory chain complex I. Here we show that MCJ is essential for the production of tumor necrosis factor by macrophages in response to a variety of Toll-like receptor ligands and bacteria, without affecting their phagocytic activity. Loss of MCJ in macrophages results in increased mitochondrial respiration and elevated basal levels of reactive oxygen species that cause activation of the JNK/c-Jun pathway, lead to the upregulation of the TACE (also known as ADAM17) inhibitor TIMP-3, and lead to the inhibition of tumor necrosis factor shedding from the plasma membrane...
January 1, 2015: Journal of Infectious Diseases
Zhengjun Chen, Jinzhong Lin, Chengjie Ma, Shumiao Zhao, Qunxin She, Yunxiang Liang
Many lactic acid bacteria carry different plasmids, particularly those that replicate via a theta mechanism. Here we describe Lactobacillus casei MCJ(CCTCC AB20130356), a new isolate that contains pMC11, carrying two distinct theta-type replicons. Each replicon contained an iteron in the origin of replication (oriV1 or oriV2) and a gene coding for the replicase (RepA_1 or RepB_1), both of which are essential for plasmid replication. Escherichia coli/Lactobacillus shuttle vectors were constructed with each replicon, yielding pEL5...
July 2014: Applied Microbiology and Biotechnology
Geraint J Parfitt, Yilu Xie, Mikhail Geyfman, Donald J Brown, James V Jester
Meibomian gland dysfunction (MGD) is frequent with aging and is the primary cause of dry eye disease, the most prevalent ocular complaint. We used a novel 3-D reconstruction technique, immunofluorescent computed tomography (ICT), to characterize meibomian gland keratinization and cell proliferation in a mouse model of age-related meibomian gland dysfunction (ARMGD). To visualize the changes associated with ARMGD, 5-month and 2-year old mouse eyelids were 3-D reconstructed by ICT using antibodies to cytokeratin (CK) 1, 5 and 6 and the proliferation marker Ki67...
November 2013: Aging
Anuj Bhardwaj, Natanasabapathy Velmurugan, Suma Ballal
AIM: Present study evaluated the efficacy of natural derivative irrigants, Morinda citrifolia juice (MCJ), Aloe Vera and Propolis in comparison to 1% sodium hypochlorite with passive ultrasonic irrigation for removal of the intraradicular E. faecalis biofilms in extracted single rooted human permanent teeth. MATERIALS AND METHODS: Biofilms of E. faecalis were grown on the prepared root canal walls of 60 standardized root halves which were longitudinally sectioned...
January 2013: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
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