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Helios T cell

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https://www.readbyqxmd.com/read/27880974/deep-sequencing-of-the-tcrb-repertoire-of-human-foxp3-and-foxp3-t-cells-suggests-that-they-are-completely-distinct-and-non-overlapping
#1
Amit Golding, Samuel Darko, William H Wylie, Daniel C Douek, Ethan M Shevach
Maintenance of peripheral tolerance requires a balance between auto-reactive conventional T cells (Tconv) and thymically-derived Foxp3(+) regulatory T cells (tTregs). Considerable controversy exists regarding the similarities/differences in TCR repertoires expressed by Tconv and tTregs. We generated highly purified populations of human adult and cord blood Tconv and tTregs based on the differential expression of CD25 and CD127. The purity of the sorted populations was validated by intracellular staining for Foxp3 and Helios...
November 23, 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27834805/tapirira-guianensis-aubl-extracts-inhibit-proliferation-and-migration-of-oral-cancer-cells-lines
#2
Renato José Silva-Oliveira, Gabriela Francine Lopes, Luiz Fernando Camargos, Ana Maciel Ribeiro, Fábio Vieira Dos Santos, Richele Priscila Severino, Vanessa Gisele Pasqualotto Severino, Ana Paula Terezan, Ralph Gruppi Thomé, Hélio Batista Dos Santos, Rui Manuel Reis, Rosy Iara Maciel de Azambuja Ribeiro
Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR)...
November 8, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27826566/variations-in-mrna-and-protein-levels-of-ikaros-family-members-in-pediatric-t-cell-acute-lymphoblastic-leukemia
#3
Julie L Mitchell, Thomas M Yankee
BACKGROUND: Pediatric T cell acute lymphoblastic leukemia (T-ALL) is a highly heterogeneous disease in which the cells share phenotypic characteristics with normal human thymocytes. The Ikaros family of transcription factors includes five members that are required for normal T cell development and are implicated in leukemogenesis. The goal of this work was to correlate the pattern of expression of Ikaros family members with the phenotype of the T-ALL cells. METHODS: We obtained twenty-four samples from pediatric T-ALL patients and used multi-parameter flow cytometry to characterize each sample, comparing the phenotype of the leukemic cells with normal human thymocytes...
October 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/27812545/low-dose-il-2-selectively-activates-subsets-of-cd4-tregs-and-nk-cells
#4
Masahiro Hirakawa, Tiago Matos, Hongye Liu, John Koreth, Haesook T Kim, Nicole E Paul, Kazuyuki Murase, Jennifer Whangbo, Ana C Alho, Sarah Nikiforow, Corey Cutler, Vincent T Ho, Philippe Armand, Edwin P Alyea, Joseph H Antin, Bruce R Blazar, Joao F Lacerda, Robert J Soiffer, Jerome Ritz
CD4(+) regulatory T cells (CD4Tregs) play a critical role in the maintenance of immune tolerance and prevention of chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. IL-2 supports the proliferation and survival of CD4Tregs and previous studies have demonstrated that IL-2 induces selective expansion of CD4Tregs and improves clinical manifestations of chronic GVHD. However, mechanisms for selective activation of CD4Tregs and the effects of low-dose IL-2 on other immune cells are not well understood...
November 3, 2016: JCI Insight
https://www.readbyqxmd.com/read/27798682/distinctive-regulatory-t-cells-and-altered-cytokine-profile-locally-in-the-airways-of-young-smokers-with-normal-lung-function
#5
Mahyar Ostadkarampour, Malin Müller, Johan Öckinger, Susanna Kullberg, Anders Lindén, Anders Eklund, Johan Grunewald, Jan Wahlström
Smoking influences the immune system in different ways and, hypothetically, effects on pulmonary effector and regulatory T cells emerge as potentially detrimental. Therefore, we characterized the frequencies and characteristics of CD4+ and CD8+ T cell subsets in the blood and lungs of young tobacco smokers. Bronchoalveolar lavage (BAL) and peripheral blood were obtained from healthy moderate smokers (n = 18; 2-24 pack-years) and never-smokers (n = 15), all with normal lung function. Cells were stimulated ex vivo and key intracellular cytokines (IFNγ, IL-17, IL-10 and TNFα) and transcription factors (Foxp3, T-bet and Helios) were analyzed using flow cytometry...
2016: PloS One
https://www.readbyqxmd.com/read/27743128/new-automated-indirect-immunofluorescent-antinuclear-antibody-testing-compares-well-with-established-manual-immunofluorescent-screening-and-titration-for-antinuclear-antibody-on-hep-2-cells
#6
M Daves, J Blecken, T Matthias, A Frey, V Perkmann, A Dall Acqua, A Joos, S Platzgummer
The IIF using the HEp-2 cell substrate should be still considered the "gold standard" techniques for determination of antinuclear antibody (ANA). Standardization and automation can be considered to be still in progress. Aim of this study was to evaluate the performance of the commercially automated indirect immunofluorescent antinuclear HEp-2 antibody assay. The study was designed to compare two commercially available HEp-2 ANA by indirect immunofluorescent antibody assays using a sensitivity panel (120 clinically determined patients) and a specificity panel consisting of 78 clinically confirmed negative patients...
October 14, 2016: Immunologic Research
https://www.readbyqxmd.com/read/27681508/alternative-splice-variants-modulates-dominant-negative-function-of-helios-in-t-cell-leukemia
#7
Shaorong Zhao, Wei Liu, Yinghui Li, Pengjiang Liu, Shufang Li, Daolei Dou, Yue Wang, Rongcun Yang, Rong Xiang, Feifei Liu
The molecular defects which lead to multistep incidences of human T-cell leukemia have yet to be identified. The DNA-binding protein Helios (known as IKZF2), a member of the Ikaros family of Krüppel-like zinc-finger proteins, functions pivotally in T-cell differentiation and activation. In this study, we identify three novel short Helios splice variants which are T-cell leukemic specific, and demonstrate their dominant-negative function. We then test the cellular localization of distinct Helios isoforms, as well as their capability to form heterodimer with Ikaros, and the association with complexes comprising histone deacetylase (HDAC)...
2016: PloS One
https://www.readbyqxmd.com/read/27669730/anti-thymocyte-globulin-g-csf-combination-therapy-leads-to-sustained-immunomodulatory-and-metabolic-effects-in-a-subset-of-responders-with-established-type-1-diabetes
#8
Michael J Haller, Stephen E Gitelman, Peter A Gottlieb, Aaron W Michels, Daniel J Perry, Andrew R Schultz, Maigan A Hulme, Jonathan J Shuster, Baiming Zou, Clive H Wasserfall, Amanda Posgai, Clayton E Mathews, Todd M Brusko, Mark A Atkinson, Desmond A Schatz
Low-dose anti-thymocyte globulin (ATG) + pegylated granulocyte-colony stimulating factor (G-CSF) preserves beta cell function for at least 12-months in type 1 diabetes (T1D). Herein, we describe metabolic and immunologic parameters 24-months following treatment. Patients with established T1D (duration 4-24 months) were randomized to ATG and peg-G-CSF (N=17) or placebo (N=8). Primary outcomes included AUC C-peptide following mixed-meal tolerance test (MMTT) and flow cytometry. "Responders" (12-month C-peptide ≥ baseline), "Super-responders" (24-month C-peptide ≥ baseline), and "Non-responders" (12-month C-peptide < baseline) were evaluated for biomarkers of outcome...
September 26, 2016: Diabetes
https://www.readbyqxmd.com/read/27637333/identification-of-novel-nuclear-factor-of-activated-t-cell-nfat-associated-proteins-in-t-cells
#9
Christian H Gabriel, Fridolin Gross, Martin Karl, Heike Stephanowitz, Anna Floriane Hennig, Melanie Weber, Stefanie Gryzik, Ivo Bachmann, Katharina Hecklau, Jürgen Wienands, Johannes Schuchhardt, Hanspeter Herzel, Andreas Radbruch, Eberhard Krause, Ria Baumgrass
Transcription factors of the nuclear factor of activated T cell (NFAT) family are essential for antigen-specific T cell activation and differentiation. Their cooperative DNA binding with other transcription factors, such as AP1 proteins (FOS, JUN, and JUNB), FOXP3, IRFs, and EGR1, dictates the gene regulatory action of NFATs. To identify as yet unknown interaction partners of NFAT, we purified biotin-tagged NFATc1/αA, NFATc1/βC, and NFATc2/C protein complexes and analyzed their components by stable isotope labeling by amino acids in cell culture-based mass spectrometry...
November 11, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27622025/regulatory-t-cells-with-multiple-suppressive-and-potentially-pro-tumor-activities-accumulate-in-human-colorectal-cancer
#10
Eleonora Timperi, Ilenia Pacella, Valeria Schinzari, Chiara Focaccetti, Luca Sacco, Francesco Farelli, Roberto Caronna, Gabriella Del Bene, Flavia Longo, Antonio Ciardi, Sergio Morelli, Anna Rita Vestri, Piero Chirletti, Vincenzo Barnaba, Silvia Piconese
Tregs can contribute to tumor progression by suppressing antitumor immunity. Exceptionally, in human colorectal cancer (CRC), Tregs are thought to exert beneficial roles in controlling pro-tumor chronic inflammation. The goal of our study was to characterize CRC-infiltrating Tregs at multiple levels, by phenotypical, molecular and functional evaluation of Tregs from the tumor site, compared to non-tumoral mucosa and peripheral blood of CRC patients. The frequency of Tregs was higher in mucosa than in blood, and further significantly increased in tumor...
July 2016: Oncoimmunology
https://www.readbyqxmd.com/read/27592800/tigit-and-helios-are-highly-expressed-on-cd4-t-cells-in-sezary-syndrome-patients
#11
Neha Jariwala, Bernice Benoit, Andrew V Kossenkov, Landon K Oetjen, Timothy M Whelan, Christine M Cornejo, Junko Takeshita, Brian S Kim, Louise C Showe, Maria Wysocka, Alain H Rook
No abstract text is available yet for this article.
September 1, 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/27570813/contribution-of-mesenteric-lymph-nodes-and-galt-to-the-intestinal-foxp3-regulatory-t-cell-compartment
#12
Duke Geem, Vu Ngo, Akihito Harusato, Benoit Chassaing, Andrew T Gewirtz, Rodney D Newberry, Timothy L Denning
: This study showed that the absence of CCR7 or mesenteric lymph nodes/gut-associated lymphoid tissue did not appreciably impact total intestinal Foxp3+ regulatory T cell representation in the steady-state. However, mesenteric lymph nodes/GALT are required for normal peripherally induced Foxp3+ regulatory T cell differentiation in the small intestine, but not in the large intestine. BACKGROUND & AIMS: Foxp3+ regulatory T cells (Tregs) in the intestine promote immune tolerance to enteric antigens...
May 2016: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27560779/t-regulatory-lymphocytes-in-type-1-diabetes-impaired-cd25-expression-and-il-2-induced-stat5-phosphorylation-in-pediatric-patients
#13
Zuzana Parackova, Jana Kayserova, Klara Danova, Kristyna Sismova, Eva Dudkova, Zdenek Sumnik, Stanislava Kolouskova, Jan Lebl, Katerina Stechova, Anna Sediva
T regulatory cells (Tregs) are essential for maintaining tolerance and preventing autoimmune diseases, such as type 1 diabetes (T1D). In our study, we investigated CD25 + FoxP3 + Tregs and thymic FoxP3 + Helios + Tregs in large cohorts of children with T1D at onset and with long-term T1D, and further in their relatives and healthy controls. We observed significantly decreased numbers of CD25 + FoxP3 + Tregs, but not FoxP3 + Helios + Tregs, in long-term patients compared with the control group and T1D onset...
August 25, 2016: Autoimmunity
https://www.readbyqxmd.com/read/27538522/elevated-level-of-circulating-cd4-helios-foxp3-cells-in-primary-sjogren-s-syndrome-patients
#14
Chen Liu, Zhao Guan, Lei Zhao, Ying Song, Hui Wang
OBJECTIVE: This study was designed to investigate the expression of regulatory T cells in primary Sjögren's Syndrome (pSS) and to evaluate the clinical role of CD4(+)Helios(+)FoxP3(+) cells in pSS patients. METHODS: CD4(+)FoxP3(+) T cells in the peripheral blood of 39 pSS patients and 30 healthy controls were measured by flow cytometry and CD25 and Helios expression were also analyzed. The repression ability of CD4(+)CD25(hi) cells was tested in vitro. Clinical information of pSS patients was retrospectively collected and their correlations with circulating Treg cells were analyzed...
August 19, 2016: Modern Rheumatology
https://www.readbyqxmd.com/read/27502439/expression-patterns-of-ikaros-family-members-during-positive-selection-and-lineage-commitment-of-human-thymocytes
#15
Julie L Mitchell, Amara Seng, Thomas M Yankee
The Ikaros family of transcription factors is essential for normal T-cell development, but their expression pattern in human thymocytes remains poorly defined. Our goal is to determine how protein levels of Ikaros, Helios and Aiolos change as human thymocytes progress through the positive selection and lineage commitment stages. To accomplish this goal, we used multi-parameter flow cytometry to define the populations in which positive selection and lineage commitment are most likely to occur. After human thymocytes express CD3 and receive positive selection signals, the cells down-regulate expression of CD4 to become transitional single-positive (TSP) CD8(+) thymocytes...
December 2016: Immunology
https://www.readbyqxmd.com/read/27479438/re-stable-inhibitory-activity-of-regulatory-t-cells-requires-the-transcription-factor-helios
#16
Anthony Atala
No abstract text is available yet for this article.
August 2016: Journal of Urology
https://www.readbyqxmd.com/read/27462886/the-destruction-complex-of-beta-catenin-in-colorectal-carcinoma-and-colonic-adenoma
#17
Guilherme Muniz Bourroul, Hélio José Fragoso, José Walter Feitosa Gomes, Vivian Sati Oba Bourroul, Celina Tizuko Fujiyama Oshima, Thiago Simão Gomes, Gabriela Tognini Saba, Rogério Tadeu Palma, Jaques Waisberg
OBJECTIVE: To evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catetenin, adenomatous polyposis coli, GSK3β, axin and ubiquitin in colorectal carcinoma and colonic adenoma. METHODS: Tissue samples from 64 patients with colorectal carcinoma and 53 patients with colonic adenoma were analyzed. Tissue microarray blocks and slides were prepared and subjected to immunohistochemistry with polyclonal antibodies in carcinoma, adjacent non-neoplastic mucosa, and adenoma tissues...
April 2016: Einstein
https://www.readbyqxmd.com/read/27456241/helios-should-not-be-cited-as-a-marker-of-human-thymus-derived-tregs-commentary-helios-and-helios-cells-coexist-within-the-natural-foxp3-t-regulatory-cell-subset-in-humans
#18
COMMENT
https://www.readbyqxmd.com/read/27424664/ex-vivo-expanded-regulatory-t-cells-cd4-cd25-foxp3-cd127-low-develop-strong-immunosuppressive-activity-in-patients-with-remitting-relapsing-multiple-sclerosis
#19
Gelena V Lifshitz, Dmitry D Zhdanov, Anastasia V Lokhonina, Daria D Eliseeva, Elena Y Lyssuck, Igor A Zavalishin, Svetlana N Bykovskaia
Multiple sclerosis (MS) is an autoimmune disease characterized by defect in regulatory function of CD4(+)CD25(+) T cells. We demonstrated difference in proportion of regulatory T cells CD4(+)CD25(+)FoxP3(+)CD127(low) (Tregs) within the same patients' relapse and remission. Proportion of peripheral Tregs (pTregs) dropped almost two times in the relapse compare to remission. Levels of pTregs in patients' remission were lower than in healthy donors. Suppressive ability of pTregs was decreased in MS patients compared to healthy donors...
July 16, 2016: Autoimmunity
https://www.readbyqxmd.com/read/27333806/tgf-%C3%AE-expressing-mesenchymal-stem-cells-induce-local-tolerance-in-a-rat-liver-transplantation-model-of-acute-rejection
#20
Jincao Tang, Renjie Yang, Ling Lv, Aihua Yao, Liyong Pu, Aihong Yin, Xiangcheng Li, Yue Yu, Scott L Nyberg, Xuehao Wang
Acute rejection is commonly encountered for long-term survival in liver transplant (LT) recipients and may impact their long-term survival if rejection is severe or recurrent. The aim of this study is to examine the therapeutic potential of transforming growth factor (TGF-β)-overexpressing mesenchymal stem cells (MSCs) in inducing a local immunosuppression in liver grafts after transplantation. MSCs were transduced with a lentiviral vector expressing the human TGF-β1 gene; TGF-β1-overexpressing MSCs (designated as TGF/MSCs) were then transfused into the liver grafts via the portal vein of a rat LT model of acute rejection...
June 22, 2016: Stem Cells
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