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https://www.readbyqxmd.com/read/29332258/photodynamic-antimicrobial-chemotherapy-pact-using-toluidine-blue-inhibits-both-growth-and-biofilm-formation-by-candida-krusei
#1
Bruna Graziele Marques da Silva, Moisés Lopes Carvalho, Isabela Bueno Rosseti, Stella Zamuner, Maricilia Silva Costa
Among non-albicans Candida species, the opportunistic pathogen Candida krusei emerges because of the high mortality related to infections produced by this yeast. The Candida krusei is an opportunistic pathogen presenting an intrinsic resistance to fluconazol. In spite of the reduced number of infections produced by C. krusei, its occurrence is increasing in some groups of patients submitted to the use of fluconazol for prophylaxis. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells...
January 13, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29331661/effect-of-laf3-ag-fluorescent-nanoparticles-on-photodynamic-efficiency-and-cytotoxicity-of-protoporphyrin-ix-photosensitizer
#2
F Tavakkoli, M Zahedifar, E Sadeghi
LaF3: Ag nanoparticles (NPs) were synthesized by the co-precipitation method. The produced NPs were characterized by X-ray diffraction (XRD) pattern, scanning electron microscope (SEM), dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FTIR). The emission spectrum of LaF3:Ag NPs is mostly overlapped with the absorption band of protoporphyrin IX (PpIX) and their conjugation was confirmed by studying fluorescence resonance energy transfer (FRET) from LaF3:Ag donor to protoporphyrin IX acceptor...
January 10, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29258951/use-of-photodynamic-therapy-in-the-treatment-of-bovine-subclinical-mastitis
#3
Lívia Helena Moreira, José Carlos Pereira de Souza, Carlos José de Lima, Miguel Angel Castillo Salgado, Adriana Barrinha Fernandes, Dora Inés Kozusny Andreani, Antonio Balbin Villaverde, Renato Amaro Zângaro
BACKGROUND: Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. METHODS: Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy - PDT, light irradiation - LED, and photosensitizer - PS)...
December 16, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29247704/increased-anti-biofilm-efficacy-of-toluidine-blue-on-staphylococcus-species-after-nano-encapsulation
#4
Bishakh Rout, Chi-Hsien Liu, Wei-Chi Wu
BACKGROUND: Photodynamic therapy has been studied by many studies as a method for inactivating bacterial growth. Workers have used planktonic bacterial as well as biofilm bacterial cultures to evaluate the potential of photodynamic therapy in inactivating bacteria. However, almost all the studies use a photosensitiser in aqueous solution, which could be detrimental to the efficiency of photodynamic therapy. METHODS: In this study, the photodynamic killing effect of toluidine blue O (TBO) has been investigated on Staphylococcal biofilms in -vitro...
December 13, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29234304/hypothesis-on-the-role-of-cryptochromes-in-inflammation-and-subarachnoid-hemorrhage-outcome
#5
Adriano Barreto Nogueira, Ariel Barreto Nogueira, José Carlos Esteves Veiga, Manoel Jacobsen Teixeira
We have recently found that the temperature variability (TV) in the day-night cycle may predict the mean intracranial pressure in the following 24 h (ICP24) in subarachnoid hemorrhage (SAH) patients under multimodality monitoring, sedation, and hypothermia (<35°C). Specifically, we found that ICP24 = 6 (4 - TV) mmHg. TV is the ratio between the coefficient of variation of temperature during the nocturnal and the preceding diurnal periods. This result suggests that the circadian clock reflects brain plasticity mechanisms and its malfunctioning leads to deterioration of the neurologic status...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/29222482/poly-methyl-vinyl-ether-alt-maleic-acid-and-ethyl-monoester-as-building-polymers-for-drug-loadable-electrospun-nanofibers
#6
Amalia Mira, C Reyes Mateo, Ricardo Mallavia, Alberto Falco
New biomaterials are sought for the development of bioengineered nanostructures. In the present study, electrospun nanofibers have been synthesized by using poly(methyl vinyl ether-alt-maleic acid) and poly(methyl vinyl ether-alt-maleic ethyl monoester) (PMVEMA-Ac and PMVEMA-ES, respectively) as building polymers for the first time. To further functionalize these materials, nanofibers of PMVEMA-Ac and PMVEMA-ES containing a conjugated polyelectrolyte (HTMA-PFP, blue emitter, and HTMA-PFNT, red emitter) were achieved with both forms maintaining a high solid state fluorescence yield without altered morphology...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29221859/photodynamic-antimicrobial-chemotherapy-pact-using-toluidine-blue-o-inhibits-the-viability-of-biofilm-produced-by-candida-albicans-at-different-stages-of-development
#7
Ana Paula Pinto, Isabela Bueno Rosseti, Moisés Lopes Carvalho, Bruna Graziele Marques da Silva, Carlos Alberto-Silva, Maricilia Silva Costa
BACKGROUND: Candida albicans is an opportunistic fungus producing both superficial and systemic infections, especially in immunocompromised individuals. It has been demonstrated that C. albicans ability to form biofilms is a crucial process for colonization and virulence. Furthermore, a correlation between the development of drug resistance and biofilm maturation at Candida biofilms has been shown. Photodynamic Antimicrobial Chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a non-toxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells...
December 5, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29213254/blue-light-therapy-following-mild-traumatic-brain-injury-effects-on-white-matter-water-diffusion-in-the-brain
#8
Sahil Bajaj, John R Vanuk, Ryan Smith, Natalie S Dailey, William D S Killgore
Mild traumatic brain injury (mTBI) is a common and often inconspicuous wound that is frequently associated with chronic low-grade symptoms and cognitive dysfunction. Previous evidence suggests that daily blue wavelength light therapy may be effective at reducing fatigue and improving sleep in patients recovering from mTBI. However, the effects of light therapy on recovering brain structure remain unexplored. In this study, we analyzed white matter diffusion properties, including generalized fractional anisotropy, and the quantity of water diffusion in isotropic (i...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/29166921/identification-of-epidermal-growth-factor-receptor-positive-glioblastoma-using-lipid-encapsulated-targeted-superparamagnetic-iron-oxide-nanoparticles-in-vitro
#9
Huai-Lu Chen, Fei-Ting Hsu, Yu-Chieh Jill Kao, Hua-Shan Liu, Wan-Zhen Huang, Chia-Feng Lu, Ping-Huei Tsai, Ahmed Atef Ahmed Ali, Gilbert Aaron Lee, Ray-Jade Chen, Cheng-Yu Chen
BACKGROUND: Targeted superparamagnetic iron oxide (SPIO) nanoparticles have emerged as a promising biomarker detection tool for molecular magnetic resonance (MR) image diagnosis. To identify patients who could benefit from Epidermal growth factor receptor (EGFR)-targeted therapies, we introduce lipid-encapsulated SPIO nanoparticles and hypothesized that anti-EGFR antibody cetuximab conjugated of such nanoparticles can be used to identify EGFR-positive glioblastomas in non-invasive T2 MR image assays...
November 22, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29166495/photodynamic-action-of-the-red-laser-on-propionibacterium-acnes
#10
Rogério Rodrigo Ramos, Jeferson Leandro de Paiva, José Paulo Franco Dos Santos Gomes, Nagib Pezati Boer, José Maria Pereira de Godoy, Fernando Batigalia
BACKGROUND: Photodynamic therapy is a therapeutic modality that has consolidated its activity in the photooxidation of organic matter, which arises from the activity of reactive oxygen species. OBJECTIVE: To evaluate the effect of red laser 660nm with the photosensitizer methylene blue on Propionibacterium acnes in vitro. METHOD: The experimental design was distributed into four groups (1 - control group without the application of light and without photosensitizer, 2 - application of light, 3 - methylene blue without light, and 4 - methylene blue with light)...
September 2017: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/29139000/effect-of-light-polarization-on-the-efficiency-of-photodynamic-therapy-of-basal-cell-carcinomas-an-in-vitro-cellular-study
#11
M JalalKamali, S N Nematollahi-Mahani, M Shojaei, A Shamsoddini, N Arabpour
In an in vitro study, the effect of light polarization on the efficiency of 5-aminolaevulinic acid (ALA) photodynamic therapy (PDT) of basal cell carcinoma (BCC) was investigated. Three states of light polarization (non-polarized, linearly polarized, and circularly polarized) were considered. Cells were exposed to green (532 pm 20 nm) irradiation from light emitting diodes. Cell survival was measured by the colorimetric assay (WST-1) and Trypan blue staining. The colorimetric assay showed a pronounced decrease in the cell viability (up to 30%) using polarized light compared to the non-polarized one in the wavelength region used...
November 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29131564/light-emitting-photon-upconversion-nanoparticles-in-the-generation-of-transdermal-reactive-oxygen-species
#12
Martin Prieto, Alina Rwei, Teresa Alejo, Wei Tuo, Maria Teresa Lopez-Franco, Gracia Mendoza, Victor Sebastian, Daniel S Kohane, Manuel Arruebo
Common photosensitizers used in photodynamic therapy do not penetrate the skin effectively. In addition, the visible blue and red lights used to excite such photosensitizers have shallow penetration depths through tissue. To overcome these limitations, we have synthesized ultraviolet and visible light emitting, energy-transfer based upconversion nanoparticles, and co-encapsulated them inside PLGA-PEG nanoparticles with photosensitizer protoporphyrin IX. Nd3+ has been introduced as sensitizer in the upconversion nanostructures to allow their excitation at 808 nm...
November 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29126958/effects-of-led-based-photodynamic-therapy-using-red-and-blue-lights-with-natural-hydrophobic-photosensitizers-on-human-glioma-cell-line
#13
Zahra Jamali, Sedigheh Marjaneh Hejazi, Seyed Mohsen Ebrahimi, Hemen Moradi-Sardareh, Maliheh Paknejad
Photodynamic therapy (PDT) has received high attention in cancer treatment due to its minimal side effects, specific cancer-targeting, non-invasion and low cost. It utilizes a specific group of anti-cancer drugs called photosensitizers (PS), which can be only activated under a certain wavelength light illumination and kills cancer cells. To screen the potential of PS and setup of PDT treatment protocol, it is essential to assess the PDT efficacy in vitro. In this study, a light-emitting diode- (LED-) based illumination system at two wavelengths (red & blue) with homogeneous and stable irradiation, and constant temperature conditions in 96-well plates was provided...
November 7, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29119936/recent-patents-on-light-based-anti-infective-approaches
#14
Imran Ahmed, Yanyan Fang, Min Lu, Quan Yan, Ahmed Mohamed Kamel El-Hussein, Michael R Hamblin, Tianhong Dai
BACKGROUND: Antibiotic resistance is one of the most serious health threats to modern medicine. The lack of potent antibiotics puts us at a disadvantage in the fight against infectious diseases, especially those caused by antibiotic-resistant microbial strains. To this end, an urgent need to search for alternative antimicrobial approaches has arisen. In the last decade, light-based therapy has made significant strides in this fight to combat antibiotic resistance among various microbial strains...
November 7, 2017: Recent Patents on Anti-infective Drug Discovery
https://www.readbyqxmd.com/read/29095531/low-level-red-led-light-inhibits-hyperkeratinization-and-inflammation-induced-by-unsaturated-fatty-acid-in-an-in-vitro-model-mimicking-acne
#15
Wen-Hwa Li, Ali Fassih, Curt Binner, Ramine Parsa, Michael D Southall
BACKGROUND AND OBJECTIVE: Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm(2) have demonstrated marked effects on inflammatory acne lesions...
November 2, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29071031/the-effect-of-combination-of-red-infrared-and-blue-wavelengths-of-low-level-laser-on-reduction-of-abdominal-girth-a-before-after-case-series
#16
Katayoon Montazeri, Soheila Mokmeli, Maryam Barat
Introduction: The objective of this study was to assess the efficacy of a combination of 3 wavelengths (including red, infra-red, and blue) of low-level laser (LLL) as a non-invasive therapeutic method to reduce abdominal girth. To achieve biochemical activity on adipocytes, a red laser was used in our selective laser combination. Near-infrared laser was used to increase depth of penetration. Nitrosyl complexes of hemoglobin (NO-Hb) are sensitive to blue light, thereby leading to increase in release of biologically active nitric oxide (NO), which can affect tissue perfusion...
2017: Journal of Lasers in Medical Sciences
https://www.readbyqxmd.com/read/29064662/near-infrared-light-triggered-upconversion-optogenetic-nanosystem-for-cancer-therapy
#17
Bin Zheng, Hanjie Wang, Huizhuo Pan, Chao Liang, Wanying Ji, Li Zhao, Hongbin Chen, Xiaoqun Gong, Xiaoli Wu, Jin Chang
In vivo the application of optogenetic manipulation in deep tissue is seriously obstructed by the limited penetration depth of visible light that is continually applied to activate a photoactuator. Herein, we designed a versatile upconversion optogenetic nanosystem based on a blue-light-mediated heterodimerization module and rare-earth upconversion nanoparticles (UCNs). The UCNs worked as a nanotransducer to convert external deep-tissue-penetrating near-infrared (NIR) light to local blue light to noninvasively activate photoreceptors for optogenetic manipulation in vivo...
October 30, 2017: ACS Nano
https://www.readbyqxmd.com/read/29064222/ex-vivo-assessment-of-photodynamic-therapy-in-achieving-microbial-reduction
#18
Rodrigo Rodrigues Amaral, Eduardo Nunes, Maria Eugênia Alvarez-Leite, José Cláudio Faria Amorim, Martinho Campolina Rebello Horta, Maria Ilma de Sousa Cortês, Frank Ferreira Silveira, Stephen Cohen
The study was to assess the effects of photodynamic therapy (PDT) on root canals contaminated by Enterococcus faecalis (E. faecalis). PDT was performed with 0.005% methylene blue dye and with a low-intensity red emission laser. Microbiological samples were collected before, immediately and 72 hours after instrumentation. Although PDT performed with the chosen light parameters promoted additional microbial reduction, this therapy did not achieve a statistically significant reduction of E. faecalis.
March 2017: Journal of the California Dental Association
https://www.readbyqxmd.com/read/29063751/tannic-acid-fe-3-ag-nanofilm-exhibiting-superior-photodynamic-and-physical-antibacterial-activity
#19
Ziqiang Xu, Xiuhua Wang, Xiangmei Liu, Zhenduo Cui, Xianjin Yang, Kelvin Wai Kwok Yeung, Jonathan Chiyuen Chung, Paul K Chu, Shuilin Wu
Silver nanoparticles (AgNPs) enwrapped in the biologically safe tannic acid (TA)/Fe(3+) nanofilm are synthesized by an ultrafast, green, simple, and universal method. The physical antibacterial activity and photodynamic antibacterial therapy (PAT) efficacy of the TA/Fe(3+)/AgNPs nanofilm were investigated for the first time, which exhibited a strong physical antibacterial activity as well as great biocompatibility, through in vitro and in vivo studies. The results disclosed that this hybrid coating could possess high PAT capabilities upon irradiation under a visible light of 660 nm, which is longer than those of previously reported green and blue sensitization light, thus allowing deeper light penetration into biological tissues...
November 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29061014/-enhancement-of-thermal-damage-to-epcam-positive-tumor-cells-by-novel-aptamer-guided-magnetic-nanoparticles
#20
L Y Zhang, M Wang, H B Wu, Y Yang, Q Li, H L Sun
Objective: To explore the thermal damage to epithelial cell adhesion molecule(EpCAM)-positive tumor cells by novel aptamer-guided magnetic nanoparticles(AptNPs). Methods: EpCAM aptamer SYL3C was connected to NPs via biotin-streptavidin reaction. The diameter of AptNPs were characterized by Dynamic Light Scattering(DLS). The binding feature of the aptamer to EpCAM-positive tumor cells was evaluated by Prussian blue dyeing. Thermal damage under alternative magnetic field was measured bylactate dehydrogenase (LDH)...
October 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
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