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https://www.readbyqxmd.com/read/28638399/recent-genetic-gains-in-nitrogen-use-efficiency-in-oilseed-rape
#1
Andreas Stahl, Mara Pfeifer, Matthias Frisch, Benjamin Wittkop, Rod J Snowdon
Nitrogen is essential for plant growth, and N fertilization allows farmers to obtain high yields and produce sufficient agricultural commodities. On the other hand, nitrogen losses potentially cause adverse effects to ecosystems and to human health. Increasing nitrogen use efficiency (NUE) is vital to solve the conflict between productivity, to secure the demand of a growing world population, and the protection of the environment. To ensure this, genetic improvement is considered to be a paramount aspect toward ecofriendly crop production...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28635260/development-validation-and-inter-laboratory-evaluation-of-a-quantitative-multiplexing-method-to-assess-levels-of-ten-endogenous-allergens-in-soybean-seed-and-its-application-to-field-trials-spanning-three-growing-seasons
#2
Ryan Christopher Hill, Trent J Oman, Xiujuan Wang, Guomin Shan, Barry Schafer, Rod A Herman, Rowel Tobias, Jeff Shippar, Bhaskar Malayappan, Li Sheng, Austin Xu, Jason Bradshaw
As part of the regulatory approval process in Europe, comparison of endogenous soybean allergen levels between genetically engineered (GE) and non-GE plants has been requested. A quantitative multiplex analytical method using tandem mass spectrometry has been developed and validated to measure ten potential soybean allergens from soybean seed. The analytical method was implemented at six laboratories to demonstrate the robustness of the method and further applied to three soybean field studies across multiple growing seasons (including 21 non-GE soybean varieties) to assess the natural variation of allergen levels...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28632115/flaviaesturariibacter-luteus-sp-nov-isolated-from-an-agricultural-floodplain-soil-and-emended-description-of-the-genus-flaviaesturariibacter
#3
Javier Pascual, Bärbel U Foesel, Alicia Geppert, Katharina J Huber, Jörg Overmann
A novel slow-growing bacterial strain designated as AW305T was isolated from an agricultural floodplain soil located in Mashare, Kavango region, Namibia. Cells stained Gram-negative, were non-motile, non-spore-forming, coccoid to rod-shaped and did not form a capsule. Colonies were yellow-pigmented, but flexirubin-type pigments were not detected. AW305T had an aerobic chemo-organoheterotrophic metabolism, using a narrow spectrum of carbon sources for growth, with preference for complex protein substrates, organic acids and amino acids...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28630123/absence-of-the-polar-organizing-protein-popz-causes-aberrant-cell-division-in-agrobacterium-tumefaciens
#4
Matthew Howell, Alena Aliashkevich, Anne K Salisbury, Felipe Cava, Grant R Bowman, Pamela J B Brown
Agrobacterium tumefaciens is a rod-shaped bacterium that grows by polar insertion of new peptidoglycan during cell elongation. As the cell cycle progresses, peptidoglycan synthesis at the pole ceases prior to insertion of new peptidoglycan at mid-cell to enable cell division. The A. tumefaciens homolog of the Caulobacter crescentus polar organelle development protein PopZ has been identified as a growth pole marker and a candidate polar growth promoting factor. Here, we characterize the function of PopZ in cell growth and division of A...
June 19, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28629495/noviherbaspirillum-denitrificans-sp-nov-a-denitrifying-bacterium-isolated-from-rice-paddy-soil-and-noviherbaspirillum-autotrophicum-sp-nov-a-denitrifying-facultatively-autotrophic-bacterium-isolated-from-rice-paddy-soil-and-proposal-to-reclassify-herbaspirillum
#5
Satoshi Ishii, Naoaki Ashida, Hiroki Ohno, Takahiro Segawa, Shuhei Yabe, Shigeto Otsuka, Akira Yokota, Keishi Senoo
Thirty-nine denitrifying bacterial strains closely related to one another, represented by strains TSA40T and TSA66T, were isolated from rice paddy soils. Strains TSA40T and TSA66T were Gram-stain-negative, slightly curved rod-shaped, and motile by means of polar flagella. They were able to reduce nitrate, nitrite and nitrous oxide, but unable to fix atmospheric N2. While strain TSA66T was able to grow autotrophically by H2-dependent denitrification, strain TSA40T could not. Phylogenetic analysis suggested that they belong to the family Oxalobacteraceae, the order Burkholderiales in the class Betaproteobacteria...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28629494/shewanella-intestini-sp-nov-isolated-from-the-intestine-of-abalone-haliotis-diversicolor
#6
Yingbao Gai, Zhaobin Huang, Qiliang Lai, Zongze Shao
A Gram-stain-negative, rod-shaped bacterium with a singular polar flagellum, designated as strain XMDDZSB0408T, was isolated from the intestine of adult abalone, Haliotis diversicolor. Activity of oxidase was positive and catalase activity was negative. It could grow at salinities from 3 to 6 % NaCl (w/v), and pH 7-9. It had the highest sequence similarity of <96.0 % with all previously established species for the complete 16S rRNA gene (1531 bp). The results of phylogenetic analysis indicated that the strain was affiliated to the genus Shewanella and closely related to Shewanella gaetbuli TF-27T and Shewanella japonica KMM 3299T (95...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28629493/naumannella-huperziae-sp-nov-an-endophytic-actinobacterium-isolated-from-huperzia-serrata-thunb
#7
Ye Sun, Hua-Hong Chen, Hong-Min Sun, Meng-Jie Ai, Jing Su, Li-Yan Yu, Yu-Qin Zhang
An endophytic, short rod-shaped, non-motile and non-spore-forming actinobacterium, designated strain CPCC 204135T, was isolated from a surface-sterilized medicinal plant, Huperzia serrata (Thunb.), collected from Sichuan Province, south-west China. Strain CPCC 204135T was observed to grow at temperatures between 20 and 37 °C (optimum, 28-32 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-9.0 % (w/v) NaCl (optimum, 0-3 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204135T belonged to the genus Naumannella, showing the highest level of 16S rRNA gene sequence similarity with Naumannella halotolerans DSM 24323T (97...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28623110/pertussis-without-apparent-cough-in-a-disabled-girl-with-a-tracheostomy
#8
Hisataka Nozawa, Kensuke Shoji, Kazuhiro Uda, Tomoo Nakamura, Mitsuru Kubota, Akira Ishiguro, Isao Miyairi
Pertussis is characterized by intense, prolonged coughing in children often followed by a distinctive whooping sound on inspiration. However, the clinical manifestations and natural course of pertussis in disabled children are largely unknown. We experienced a case of pertussis in a disabled girl who had previously undergone a tracheostomy and laryngotracheal separation. She presented with increased tracheal secretions and required hospitalization but did not develop a cough. Pertussis was suspected from the sputum Gram stain, which revealed numerous, short gram-negative rods that did not grow on chocolate agar...
June 13, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28622904/radiographic-outcomes-of-shilla-growth-guidance-system-and-traditional-growing-rods-through-definitive-treatment
#9
Scott J Luhmann, June C Smith, Ann McClung, Frances L McCullough, Richard E McCarthy, George H Thompson
STUDY DESIGN: Retrospective review of a multicenter database. OBJECTIVES: To compare the radiographic outcomes of patients who had undergone the Shilla Growth Guidance System (SGGS) and traditional growing rod (GR) treatment for management of early-onset scoliosis (EOS) through definitive treatment. SUMMARY OF BACKGROUND DATA: The efficacy of surgical treatment of EOS can only be determined after definitive treatment has been completed. We wanted to review our experience with the SGGS and GR for management of EOS through definitive treatment...
July 2017: Spine Deformity
https://www.readbyqxmd.com/read/28622795/desulfamplus-magnetovallimortis-gen-nov-sp-nov-a-magnetotactic-bacterium-from-a-brackish-desert-spring-able-to-biomineralize-greigite-and-magnetite-that-represents-a-novel-lineage-in-the-desulfobacteraceae
#10
Elodie C T Descamps, Caroline L Monteil, Nicolas Menguy, Nicolas Ginet, David Pignol, Dennis A Bazylinski, Christopher T Lefèvre
A magnetotactic bacterium, designated strain BW-1(T), was isolated from a brackish spring in Death Valley National Park (California, USA) and cultivated in axenic culture. The Gram-negative cells of strain BW-1(T) are relatively large and rod-shaped and possess a single polar flagellum (monotrichous). This strain is the first magnetotactic bacterium isolated in axenic culture capable of producing greigite and/or magnetite nanocrystals aligned in one or more chains per cell. Strain BW-1(T) is an obligate anaerobe that grows chemoorganoheterotrophically while reducing sulfate as a terminal electron acceptor...
May 31, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28619149/abortion-and-mortality-in-farm-mink-neovison-vison-associated-with-feed-born-clostridium-limosum
#11
Anne Sofie Hammer, Lars Andresen, Bent Aalbæk, Peter Damborg, Vilhelm Weiss, Mette Line Christiansen, Søren Selsing, Martin Iain Bahl
Disease in mink clinically characterized by abortion and increased mortality among pregnant female mink on 28 Danish farms was observed during April and May 2015. Most of these farms suffered extensive disease problems, including a significant increase in the number of mated females without litters. Pathological, microbiological and molecular biological methods were applied to investigate the cause of disease. Necropsies of animals found dead revealed fragile and partially dissolved (liquefying) uterine tissue, with the presence of Gram positive rod-shaped bacteria...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28614287/nasal-swab-screening-for-staphylococcus-aureus-in-spinal-deformity-patients-treated-with-growing-rods
#12
Scott J Luhmann, June C Smith
BACKGROUND: Surgical-site infections are one of the most concerning complications in patients treated with growing rods (GR). The purpose of this study was to evaluate the use of preoperative screening for Staphylococcus aureus (SA) for all growing spine procedures, and if this would permit alteration of prophylactic antibiotics to cover the identified resistances. METHODS: All patients were identified who had SA screening during the course of GR treatment. In otal, 34 patients [23 neuromuscular (NMS), 4 congenital, 4 idiopathic scoliosis (IS), and 3 syndromic] were identified who had 111 preoperative screenings [79 lengthenings, 23 insertions, 6 revisions, and 3 conversions to posterior spinal fusions (PSF)]...
June 13, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28613148/sphingopyxis-solisilvae-sp-nov-isolated-from-forest-soil
#13
Dhiraj Kumar Chaudhary, Ram Hari Dahal, Jaisoo Kim
A yellow-coloured, Gram-staining-negative, motile and rod shaped bacterium, designated strain R366T, was isolated from forest soil of Kyonggi University, South Korea. It was able to grow at 15-42 °C, pH 6.0-10.0 and with 0-4 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain R366T belongs to the genus Sphingopyxis and is closely related to Sphingopyxis italica SC13E-S71T (98.72 % sequence similarity), Sphingopyxis chilensis S37T (98.51 %), Sphingopyxis fribergensis Kp5...
June 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28608177/ten-year-follow-up-of-jarcho-levin-syndrome-with-thoracic-insufficiency-treated-by-veptr-and-mcgr-veptr-hybrid
#14
Kenny Yat Hong Kwan, Jason Pui Yin Cheung, Karen Kar Lum Yiu, Kenneth Man Chee Cheung
PURPOSE: Jarcho-Levin syndrome patients commonly suffer from repeated respiratory infections and become ventilator dependent due to an insufficient thoracic volume. Multiple congenital vertebral anomalies are associated with this genetic disorder and results in stunted spine growth. The purpose of this case report is to present the results of a hybrid vertical expandable prosthetic titanium rib (VEPTR) and magnetically controlled growing rod (MCGR) construct for the management of a patient with Jarcho-Levin syndrome...
June 12, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28604490/health-related-quality-of-life-in-early-onset-scoliosis-patients-treated-surgically-eosq-scores-in-traditional-growing-rod-vs-magnetically-controlled-growing-rods
#15
Michael E Doany, Z Deniz Olgun, Gizem Irem Kinikli, Senol Bekmez, Aykut Kocyigit, Gokhan Demirkiran, A Ergun Karaagaoglu, Muharrem Yazici
STUDY DESIGN: Cross-sectional study. OBJECTIVE: To compare quality of life and caregiver burden in TGR and MCGR patients. SUMMARY OF BACKGROUND DATA: Magnetic controlled growing rods (MCGR) decrease surgical sessions associated with treatment of early onset scoliosis (EOS), hoping to minimize the burdens seen with repetitive invasive surgeries in traditional growing rod (TGR) treatment. Although the clinical indications for these treatments have largely been agreed upon, there is a lack of understanding of their impact on patients' and families' quality of life...
June 9, 2017: Spine
https://www.readbyqxmd.com/read/28600908/a-rapid-qualitative-assay-for-detection-of-clostridium-perfringens-in-canned-food-products
#16
Gayatri Ashwinkumar Dave
Clostridium perfringens (MTCC 1349) is a Gram-positive, anaerobic, endospore forming, and rod-shaped bacterium. This bacterium produces a variety of toxins under strict anaerobic environment. C. perfringens can grow at temperatures ranging between 20°C and 50°C. It is the major causetive agent for gas gangrene, cellulitis, septicemia, necrotic enteritis and food poisoning, which are common toxin induced conditions noted in human and animals. C. perfringens can produce produce four major types of toxins that are used for the classification of strains, classified under type A-E...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28598301/aquimarina-rubra-sp-nov-isolated-from-sediment-of-a-sea-cucumber-culture-pond
#17
Ji-Ru Han, Dong-Bo Fang, Hai-Feng Xia, Guan-Jun Chen, Zong-Jun Du
A Gram-stain-negative, non-motile, rod-shaped, red-pigmented, facultatively anaerobic bacterium, designated SS2-9T, was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, China. Cells of strain SS2-9T were approximately 0.3-0.5 µm in width and 1.5-6.0 µm in length. The strain was able to grow at 10-37 °C, at pH 6.5-8.5 and in the presence of 0.5-6.0 % (w/v) NaCl. It grew optimally at 28 °C and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content was 34...
June 9, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28594523/congenital-scoliosis-a-case-based-approach
#18
Frances A Farley, Laurel C Blakemore
Congenital scoliosis is lateral curvature of the spine caused by vertebral anomalies. Congenital scoliosis is associated with congenital anomalies of other organ systems. Traditionally, treatment options for patients with congenital scoliosis focused on posterior spinal fusion. Current surgical treatment options for young children include growing rods, vertical expandable prosthetic titanium ribs, and, most recently, magnetic rods. Hemivertebrae resection is an important early treatment option for patients who have a hemivertebra...
February 15, 2017: Instructional Course Lectures
https://www.readbyqxmd.com/read/28592660/mechanical-interactions-in-bacterial-colonies-and-the-surfing-probability-of-beneficial-mutations
#19
Fred D Farrell, Matti Gralka, Oskar Hallatschek, Bartlomiej Waclaw
Bacterial conglomerates such as biofilms and microcolonies are ubiquitous in nature and play an important role in industry and medicine. In contrast to well-mixed cultures routinely used in microbial research, bacteria in a microcolony interact mechanically with one another and with the substrate to which they are attached. Here, we use a computer model of a microbial colony of rod-shaped cells to investigate how physical interactions between cells determine their motion in the colony and how this affects biological evolution...
June 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28589952/contrasting-mechanisms-of-growth-in-two-model-rod-shaped-bacteria
#20
Cyrille Billaudeau, Arnaud Chastanet, Zhizhong Yao, Charlène Cornilleau, Nicolas Mirouze, Vincent Fromion, Rut Carballido-López
How cells control their shape and size is a long-standing question in cell biology. Many rod-shaped bacteria elongate their sidewalls by the action of cell wall synthesizing machineries that are associated to actin-like MreB cortical patches. However, little is known about how elongation is regulated to enable varied growth rates and sizes. Here we use total internal reflection fluorescence microscopy and single-particle tracking to visualize MreB isoforms, as a proxy for cell wall synthesis, in Bacillus subtilis and Escherichia coli cells growing in different media and during nutrient upshift...
June 7, 2017: Nature Communications
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