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Growing rod

Yue-Qiang Wang, Xiao-Ming Liu, Jia Tang, Gui-Qin Yang, Zhen Yu
A novel halophilic bacterium, strain GSS13(T), capable of growing at salinities of 8-28 % (w/v) NaCl (optimally at 24 %, w/v) was isolated from Yuncheng Saline Lake in China. GSS13(T) was Gram-stain-positive, strictly aerobic, rod-shaped, motile and a non-spore-former. Growth occurred at pH 5.5-8.5 (optimum pH 7.0) and at 10-45 °C (optimum 30 °C). On the basis of the results of 16S rRNA gene sequences phylogenetic analyses, GSS13(T) represents a member of the genus Salibacterium and is closely related to Salibacterium halotolerans S7(T), Salibacterium qingdaonense CM1(T) and Salibacterium halochares MSS4(T), with 16S rRNA gene sequence similarities of 98...
November 17, 2017: International Journal of Systematic and Evolutionary Microbiology
Dhiraj Kumar Chaudhary, Ram Hari Dahal, Jaisoo Kim
Two novel strains, J116-2(T) and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. Strains J116-2(T) and J116-1 were able to grow at 20-32 °C, pH 6.0-8.5, and 0-0.5 % (w/v) NaCl concentration. Based on 16S rRNA gene sequence analyses, strains J116-2(T) and J116-1 formed a distinct lineage within the family Chitinophagaceae of the phylum Bacteroidetes and were closely related to genera Taibaiella (89...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Romain Grangeon, John Zupan, Yeonji Jeon, Patricia C Zambryski
Agrobacterium tumefaciens grows by addition of peptidoglycan (PG) at one pole of the bacterium. During the cell cycle, the cell needs to maintain two different developmental programs, one at the growth pole and another at the inert old pole. Proteins involved in this process are not yet well characterized. To further characterize the role of pole-organizing protein A. tumefaciens PopZ (PopZ At ), we created deletions of the five PopZ At domains and assayed their localization. In addition, we created a popZAt deletion strain (ΔpopZAt ) that exhibited growth and cell division defects with ectopic growth poles and minicells, but the strain is unstable...
November 14, 2017: MBio
Thriveni G Anjali, Madivala G Basavaraj
The superior stability of Pickering emulsions can be detrimental in several applications. There is therefore growing interest in using stimuli responsive particle emulsifiers that can considerably reduce the cost of demulsification. Among the possible triggers for the remote control of emulsion stability, pH sensitive systems are advantageous because of the simplicity and ease of implementation. Herein, the destabilization mechanism of pH-switchable emulsions stabilized solely by particles of different shapes and surface properties - namely - hematite, silica and polystyrene - are discussed...
November 14, 2017: Physical Chemistry Chemical Physics: PCCP
Mojtaba Mohseni, Jaber Abbaszadeh, Shima-Sadat Maghool, Mohammad-Javad Chaichi
Monitoring and assessing toxic materials which are being released into the environment along with wastewater is a growing concern in many industries. The current research describes a highly sensitive and rapid method for the detection of toxic concentrations of heavy metals in aquatic environments. Water samples were collected from southern coasts of the Caspian Sea followed by screening of luminescent bacteria. Phylogenetic analysis, including gene sequence of 16S rRNA, and biochemical tests were performed for identification of the isolate...
November 8, 2017: Ecotoxicology and Environmental Safety
Denis Ptchelkine, Ashley Gillum, Tomohiro Mochizuki, Soizick Lucas-Staat, Ying Liu, Mart Krupovic, Simon E V Phillips, David Prangishvili, Juha T Huiskonen
Archaeal viruses have evolved to infect hosts often thriving in extreme conditions such as high temperatures. However, there is a paucity of information on archaeal virion structures, genome packaging, and determinants of temperature resistance. The rod-shaped virus APBV1 (Aeropyrum pernix bacilliform virus 1) is among the most thermostable viruses known; it infects a hyperthermophile Aeropyrum pernix, which grows optimally at 90 °C. Here we report the structure of APBV1, determined by cryo-electron microscopy at near-atomic resolution...
November 10, 2017: Nature Communications
Shih-Yao Lin, Asif Hameed, Yi-Han Hsu, You-Cheng Liu, Mei-Hua Hung, Wei-An Lai, Chiu-Chung Young
A polyphasic approach was used to characterize an aerobic, Gram-stain-negative, rod-shaped bacterium (designed as strain CC-MHH0539(T)) isolated from the chopped tuber of taro (Colocasia esculanta) in Taiwan. Strain CC-MHH0539(T) was able to grow at 15-30 °C (optimum, 25 °C), at pH 6.0-9.0 (optimum, 7.0) and with 0-1 % (w/v) NaCl. Strain CC-MHH0539(T) showed highest 16S rRNA gene sequence similarity to Sphingomonas laterariae LNB2(T) (96.8 %), Sphingobium boeckii 469(T) (96.5 %), Sphingomonas faucium E62-3(T) (96...
November 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Miao-Chun Fan, Li-Jun Nan, Ya-Min Zhu, Wei-Min Chen, Ge-Hong Wei, Yan-Bing Lin
A novel endophytic bacterium, designated strain HZ7(T), was isolated from the root nodules of Robinia pseudoacacia growing in a lead-zinc mine in Mianxian County, Shaanxi Province, China. Cells were Gram-reaction-negative, aerobic, motile, rod-shaped, methyl-red-negative, catalase-positive, positive for chitosan-degrading activity and did not produce H2S. Strain HZ7(T) grew at 4-45 °C (optimum 25-30 °C), at pH 5-9 (optimum pH 7-8) and with 0-1 % (w/v) NaCl. The quinone type was ubiquinone 8 (UQ-8). The major fatty acids were identified as C16 : 0, C17 : 0 cyclo and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c)...
November 2, 2017: International Journal of Systematic and Evolutionary Microbiology
Sophie Betka, Gaby Pfeifer, Sarah Garfinkel, Hielke Prins, Rod Bond, Henrique Sequeira, Theodora Duka, Hugo Critchley
BACKGROUND: Alexithymia describes an abnormality of emotional experience that is commonly expressed among individuals with addiction and alcohol abuse disorders. Alexithymic individuals are characterized by difficulties in identifying and describing their emotions. This impairment is linked to the development and maintenance of addiction. Moreover, an emergent theory suggests alexithymia is itself secondary to a failure of interoception (sensitivity to internal bodily signals, including physiological arousal states)...
November 2, 2017: Alcoholism, Clinical and Experimental Research
Francisco Rafael Alves Santana Cesário, Thaís Rodrigues de Albuquerque, Giovana Mendes de Lacerda, Maria Rayane Correia de Oliveira, Lindaiane Bezerra Rodrigues, Anita Oliveira Brito Pereira Bezerra Martins, Aline Augusti Boligon, Lucindo José Quintans Júnior, Adriano Antunes de Souza Araújo, Mariana Lima Vale, Henrique Douglas Melo Coutinho, Irwin Rose Alencar de Menezes
Tocoyena formosa, a small ornamental tree growing in the dry regions of central Brazil, is commonly known as 'genipapo do bravo'. This is a medicinal plant traditionally used as an analgesic for rheumatic pain, lower back pain and myalgia, however its use is carried out without scientific evidence, which thus justifies the development of studies to investigate and prove its therapeutic potential. Therefore, the objective of this study was to evaluate the phytochemical profile of Tocoyena formosa (Cham. & Schlecht...
October 26, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Guang-Da Feng, Xiong Xiong, Hong-Hui Zhu, Hua-Ping Li
A difficult to cultivate bacterial strain, designated 1PNM-26(T), isolated from a lead-zinc mine, was investigated using a polyphasic taxonomic approach. The strain was able to grow on solid medium but not in liquid medium. Cells were Gram-reaction-negative, aerobic, non-spore-forming, non-motile and rod-shaped. It showed positive reactions for catalase and oxidase and hydrolysis of aesculin. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 1PNM-26(T) represents a member of the genus Sphingomonas and forms a stable cluster with Sphingomonas morindae KCTC 42183(T), Sphingomonas polyaromaticivorans JCM 16711(T) and Sphingomonas oligoaromativorans NBRC 105508(T)...
November 1, 2017: International Journal of Systematic and Evolutionary Microbiology
Francisco M Nadal-Nicolás, Manuel Vidal-Sanz, Marta Agudo-Barriuso
In healthy beings, age is the ultimate reason of cellular malfunction and death. In the rat retina, age causes a functional decline and loss of specific neuronal populations. In this regard, controversial conclusions have been reported for the innermost retina. Here, we have studied the albino and pigmented retina for the duration of the rat life-span. Independent of age (21 days-22 months), the electroretinographic recordings and the volume of the retina and its layers are smaller in albinos. Functionally, aging causes in both strains a loss of cone- and rod-mediated responses...
September 28, 2017: Neurobiology of Aging
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Two strains of bacteria, designated J107-1(T) and J107-2, were isolated from farmland soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, coccoid rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Both strains hydrolysed casein and tyrosine. Strains could grow at pH 9.0 and at 42 °C. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strains J107-1(T) and J107-2 formed a lineage within the phylum Bacteroidetes that was distinct from various members of the family Chitinophagaceae, including Ferruginibacter paludis HME8881(T) (89...
October 26, 2017: International Journal of Systematic and Evolutionary Microbiology
Kobi Flomin, Mahmud Diab, Taleb Mokari
Multi-component nanostructures of Au-CdS-ZnO with a novel morphology were synthesized by a non-conventional strategy where seeded growth is combined with solution-liquid-solid (SLS) growth. Each of these synthetic routes is used for growing a different domain of the final heterostructure, where ZnO rods are grown first on Au nanoparticles via heterogeneous nucleation while CdS is later grown between these two domains via SLS, using the Au tip of the preformed Au-ZnO as a catalyst. The in situ alloying of the Au tip with Cd enabled the metal tip to function as an SLS catalyst at a relatively mild reaction temperature which is lower than the melting point of pure Au...
November 2, 2017: Nanoscale
Theresa Mayes, Jennifer M Anadio, Peter F Sturm
BACKGROUND: Establishing prevalence of vitamin D deficiency in elective scoliosis surgery may impact clinical outcomes. The objectives of this study were to document vitamin D status of patients with scoliosis preparing for surgical intervention in order to establish frequency of deficiency and determine characteristics influencing levels. METHODS: Records were queried for patients with scoliosis diagnosis who underwent posterior spinal fusion or initial growing rod placement and had serum vitamin 25 hydroxyvitamin D (D25) recorded in the preoperative period...
November 2017: Spine Deformity
Dhiraj Kumar Chaudhary, Sang Don Lee, Jaisoo Kim
Strain K-4-11-1(T), a psychrotolerant, light salmon-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from forest soil of Kyonggi University, Suwon, South Korea. It was able to grow at 0-32 °C, at pH 5.0-10.0 and with 0-1.5 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain K-4-11-1(T) belongs to the genus Pedobacter and is closely related to Pedobacter ginsenosidimutans THG-45(T) (98.75 % sequence similarity), Pedobacter borealis G-1(T) (98...
October 19, 2017: International Journal of Systematic and Evolutionary Microbiology
Akira Hiraishi
Two strains of thermotolerant phototrophic alphaproteobacteria, designated strains TUT3542(T) and TUT3581(T), were isolated from sediment mud and cyanobacterial mats in a geothermal spring in Japan, respectively, and taxonomically characterized. Both the strains were budding motile rods and were able to grow at 45 °C. Phototrophically grown cells of strains TUT3542(T) and TUT3581(T) produced pink and brownish red cultures, respectively, and showed in vivo absorption maxima at 800, 858-859 and 892-895 nm in the near infrared region, indicating the presence of a core reaction centre and peripheral pigment complexes with bacteriochlorophyll a...
October 16, 2017: International Journal of Systematic and Evolutionary Microbiology
Hanyi Chen, Ai-Lian Piao, Xu Tan, Yuichi Nogi, Jina Yeo, Han Lu, Qing-Qing Feng, Jie Lv
A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, designated T5(T), was isolated from the Chishui River in Maotai town, Guizhou Province, Southwest of China. Strain T5(T) was found to grow optimally at pH 9.0 and 25 °C. The 16S rRNA gene sequence analysis indicated that strain T5(T) belongs to the family Sphingomonadaceae within the phylum Proteobacteria; the strain T5(T) clustered with the type strains of Sphingopyxis contaminans, Sphingorhabdus wooponensis and Sphingorhabdus rigui, with which it exhibits 16S rRNA gene sequence similarity values of 96...
October 13, 2017: Antonie Van Leeuwenhoek
Leonid N Ten, Nabil Elderiny, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, Myung Kyum Kim, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-9(T), was isolated from a sandy beach in Jeju Island, South Korea. The isolate was able to grow between 10 and 30 °C, pH 5-8, and in presence of 0-1% (w/v) NaCl. Based on 16S rRNA gene phylogenetic analysis, the novel strain was closely related to members of the genus Spirosoma (96.1-90.9% similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099(T) (96.1%). The G + C content of the genomic DNA of strain 15J8-9(T) was 45...
October 11, 2017: Current Microbiology
Mutlu Cobanoglu, Suken A Shah, Peter Gabos, Kenneth Rogers, Petya Yorgova, Geraldine Neiss, Leslie Grissom, William G Mackenzie
BACKGROUND: In the treatment of early onset scoliosis (EOS), there has been a trend to use magnetically controlled growing rods (MCGR) in order to reduce the number of surgeries. To confirm the amount of lengthening, spine radiographs were required. Recently, ultrasound (US) has been added to monitor lengthening of MCGR to avoid radiation exposure. Our aim was to determine whether US is as accurate as plain radiography (x-ray) in determining the amount of length achieved at individual MCGR lengthening episodes...
October 9, 2017: Journal of Pediatric Orthopedics
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