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Growing rod

Rod Thomson
The government has highlighted the need to address the growing demands on the NHS and social care services, stating the importance of preventing ill health and promoting well-being. However, its actions do not appear to match its rhetoric.
August 16, 2017: Nursing Standard
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Two strains of bacteria designated CB-3T and CB-31 were isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. They were able to grow at 15-42 °C, pH 5.5-9.5 and with 0-1.5 % (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains CB-3T and CB-31 formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes that was distinct from various species of the genus Flavobacterium, including Flavobacterium humicola UCM-46T (99...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Rebecca M Davidson, Mary Ann DeGroote, Jamie L Marola, Sarah Buss, Victoria Jones, Michael R McNeil, Alison G Freifeld, L Elaine Epperson, Nabeeh A Hasan, Mary Jackson, Peter C Iwen, Max Salfinger, Michael Strong
A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Christopher A Dunlap, David A Schisler, Elizabeth B Perry, Nora Connor, Frederick M Cohan, Alejandro P Rooney
Two isolates of Gram-reaction-positive, facultatively anaerobic, motile, rod-shaped, endospore-forming bacteria were identified during a survey of the diversity of strains belonging to the genus Bacillus deposited in the Agriculture Research Service Culture Collection. These strains were originally isolated from soil in Evolution Canyon III (Israel) in a survey of ecological diversification. Phylogenetic analysis of the 16S rRNA gene of strains NRRL B-41294T and NRRL B-41327T determined they were closely related to members of the Bacillus licheniformis clade...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Dhiraj Kumar Chaudhary, Jaisoo Kim
A light yellow-coloured, Gram-stain-negative, motile and rod-shaped bacterium, designated strain K-3-1T, was isolated from reclaimed grassland soils of Belbari, Morang, Nepal. It was able to grow at 4-45 °C, at pH 5.0-10.0, and at 0-2 % (w/v) NaCl concentrations. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain K-3-1T belongs to the genus Massilia and is closely related to Massilia consociata CCUG 58010T (98.3 % sequence similarity), Massilia tieshanensis TS3T (98...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Dhiraj Kumar Chaudhary, Jaisoo Kim
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped bacterium, designated K-1-16T, that is capable of degrading aliphatic hydrocarbons was isolated from oil-contaminated soil at Biratnagar, Morang, Nepal. It was able to grow at 15-45 °C, at pH 5.5-9.5 and with 0-5 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain K-1-16T belongs to the genus Sphingomonas and is closely related to Sphingomonas mucosissima CP173-2T (98...
August 10, 2017: International Journal of Systematic and Evolutionary Microbiology
María José León, Cristina Sánchez-Porro, Antonio Ventosa
A moderately halophilic bacterium designated strain M6-53T was isolated from water of a pond from a marine saltern located in Huelva, south-west Spain. Cells of the strain were Gram-stain-negative, strictly aerobic, motile, slightly curved rods, able to grow in media containing 5-25 % (w/v) NaCl (optimal growth at 10 %, w/v), at temperatures from 20 to 40 °C (optimally at 37 °C) and at pH 6.5-9 (optimally at pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences placed the new isolate within the genus Marinobacter, with the type strains of the most closely related species being Marinobacter persicus IBRC-M 10445T (98...
August 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Joshua B Holt, Lori A Dolan, Stuart L Weinstein
BACKGROUND: Spinal muscular atrophy (SMA) is a progressive neuromuscular disease commonly including progressive scoliosis resulting in severe deformity and negatively affecting pulmonary function. Surgical correction and stabilization of this progressive deformity is generally recommended; however, the timing and method of surgical fixation remains controversial. METHODS: Retrospective review of clinical, radiographic, and pulmonary function data from 16 children with SMA and surgically treated scoliosis between 1985 and 2013...
August 2, 2017: Journal of Pediatric Orthopedics
Y Subhash, Hye-Ji Kim, Sang-Seob Lee
Two bacterial strains (1-10M-8-7-50T and 1M) were isolated from lagoon sediments. The strains were obligately aerobic, Gram-stain-positive, spore-forming, rod-shaped and motile. Both strains were able to grow at 25-60 ˚C (optimum 40-42 ˚C), at pH 6.0-8.8 (optimum pH 7.0-7.5) and with 0-2.0 % (w/v) (optimum 0-0.8 %) NaCl. The predominant cellular fatty acids were iso-C15 : 0 and iso-C15 : 0 2-OH, and the cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, glycine and alanine...
August 4, 2017: International Journal of Systematic and Evolutionary Microbiology
Kristina Beblo-Vranesevic, Harald Huber, Petra Rettberg
On Mars, significant amounts (0.4-0.6%) of perchlorate ions were detected in dry soil by the Phoenix Wet Chemistry Laboratory and later confirmed with the Mars Science Laboratory. Therefore, the ability of Hydrogenothermus marinus, a desiccation tolerant bacterium, to survive and grow in the presence of perchlorates was determined. Results indicated that H. marinus was able to tolerate concentrations of sodium perchlorate up to 200 mM ( 1.6%) during cultivation without any changes in its growth pattern. After the addition of up to 440 mM ( 3...
2017: Frontiers in Microbiology
Jason Pui Yin Cheung, Karen Kar Lum Yiu, Dino Samartzis, Kenny Kwan, Boon-Beng Tan, Kenneth Man Chee Cheung
STUDY DESIGN: Prospective study OBJECTIVE.: To identify the factors that are associated with rod slippage and to study the pattern of achieved length gain with a standard distraction methodology. SUMMARY OF BACKGROUND DATA: Ability to achieve successful magnetically controlled growing rod (MCGR) distraction is crucial for gradual spine lengthening. Rod slippage has been described as a failure of internal magnet rotation leading to a slippage and an inability to distract the rod...
August 1, 2017: Spine
Dimitry Y Sorokin, Ilya V Kublanov, Tatiana V Khijniak
Brine samples from Kulunda Steppe soda lakes (Altai, Russia) were inoculated into a hypersaline alkaline mineral medium with β-keratin (chicken feather) as a substrate. The micro-organisms dominating the enrichment culture were isolated by limiting serial dilution on the same medium with casein as a substrate. The cells of strain BSker1T were motile, curved rods. The strain was an obligately aerobic heterotroph utilizing proteins and peptides as growth substrates. The isolate was an obligate alkaliphile with a pH range for growth from pH 8...
July 31, 2017: International Journal of Systematic and Evolutionary Microbiology
Pooja Manandhar, Gengxin Zhang, Arun Lama, Fei Liu, Yilun Hu
An orange pigmented, Gram-staining negative, aerobic, motile, rod-shaped bacterium isolated from a soil from the Tanggula Mountain, China was studied using a polyphasic approach. Based on 16S rRNA gene sequence similarity, strain W16RD(T) was found to be closely related to Sphingomonas prati DSM 103336(T) (99%), Sphingomonas fennica DSM 13665(T) (97.21%), followed by Sphingomonas laterariae DSM 25432(T) (96.44%), Sphingomonas haloaromaticamans CGMCC 1.10206 (T) (96.36%) and Sphingomonas formosensis DSM 24164(T) (96...
July 28, 2017: Antonie Van Leeuwenhoek
R Pechar, J Killer, R Švejstil, H Salmonová, M Geigerová, V Bunešová, V Rada, O Benada
Bacteria with potential probiotic applications are not yet sufficiently explored, even for animals with economic importance. Therefore, we decided to isolate and identify representatives of the family Bifidobacteriaceae, which inhabit the crop of laying hens. During the study, a fructose-6-phosphate phosphoketolase-positive strain, RP51T, with a regular/slightly irregular and sometimes an S-shaped slightly curved rod-like shape, was isolated from the crop of a 13 -month-old Hisex Brown hybrid laying hen. The best growth of the Gram-stain-positive bacterium, which was isolated using Bifidobacterium-selective mTPY agar, was found out to be under strictly anaerobic conditions, however an ability to grow under microaerophilic and aerobic conditions was also observed...
July 21, 2017: International Journal of Systematic and Evolutionary Microbiology
Felix Wong, Lars D Renner, Gizem Özbaykal, Jayson Paulose, Douglas B Weibel, Sven van Teeffelen, Ariel Amir
The shapes of most bacteria are imparted by the structures of their peptidoglycan cell walls, which are determined by many dynamic processes that can be described on various length scales ranging from short-range glycan insertions to cellular-scale elasticity(1-11). Understanding the mechanisms that maintain stable, rod-like morphologies in certain bacteria has proved to be challenging due to an incomplete understanding of the feedback between growth and the elastic and geometric properties of the cell wall(3,4,12-14)...
July 24, 2017: Nature Microbiology
Daiana Guevara Nuñez, Adriana N De Paulis, Eugenia Bertona, Miguel Ángel Gutiérrez, Carlos A Vay, Juan P Suárez, Silvia C Predari
Bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by CO2 and anaerobiosis. Exceptionally it has been associated with infections in humans. An elderly male patient with a urinary tract infection due to B. scardovii and Enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. The bacillus did not grow on standard media, but on chocolate agar incubated in CO2 and on supplemented Brucella agar in an anaerobic atmosphere, incubated for 72h at 35°C...
July 19, 2017: Revista Argentina de Microbiología
Kosuke Okeyoshi, Maiko K Okajima, Tatsuo Kaneko
Living organisms in drying environments build anisotropic structures and exhibit directionality through self-organization of biopolymers. However, the process of macro-scale assembly is still unknown. Here, we introduce a dissipative structure through a non-equilibrium process between hydration and deposition in the drying of a polysaccharide liquid crystalline solution. By controlling the geometries of the evaporation front in a limited space, multiple nuclei emerge to grow vertical membrane walls with macroscopic orientation...
July 21, 2017: Scientific Reports
Dhiraj Kumar Chaudhary, Jaisoo Kim
During a study of psychrophilic hydrocarbon-degrading bacteria, two yellow, Gram-staining-negative, non-motile, and rod shaped bacteria, designated R-10-9T and K-4-2 were isolated from oil-contaminated soil from Ulaanbaatar, Mongolia. Both strains were able to grow at 0-32 °C, pH 5.0-9.5, and 0-1 % (w/v) NaCl concentration. These strains were taxonomically characterized by a polyphasic approach. On the basis of the results of 16S rRNA gene sequence analysis, R-10-9T and K-4-2 belong to the genus Flavobacterium and are closely related to Flavobacterium oncorhynchi 631-08T (97...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Zhiming Liu, Wenjian Wu
Hybrid materials of metal nanoparticles and biopolymers with catalytic properties are very promising to be used as detectors in biochemical reactions. In this work, the catalytic properties and relevant <i>in-situ</i> self-assembly abilities of hybrid films of gold nanoparticles (GNPs) and cellulose for the oxidation of benign chromogen 3,3',5,5'-tetramethylbenzidine (TMB) with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) are revealed for the first time. The peroxidase-like properties of hybrid films are inherited from those of colloidal GNPs and increase with their contents of GNPs...
July 10, 2017: Nanotechnology
Hui Zhang, Ming-Jing Shi, Hai-Feng Xia, Guan-Jun Chen, Zong-Jun Du
A Gram-stain-negative, aerobic, non-gliding, rod-shaped and orange-coloured bacterium, designated strain P131T, was isolated from marine sediment of the coast of Weihai, China, and subjected to a polyphasic study. Strain P131T was found to grow optimally at 28-30 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. In a phylogenetic analysis based on 16S rRNA gene sequences, strain P131T was found to belong to the genus Bizionia and exhibited 94.6-97.0 % 16S rRNA gene sequence similarity with recognized Bizionia species...
July 8, 2017: International Journal of Systematic and Evolutionary Microbiology
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