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Growing rod

Ram Hari Dahal, Jaisoo Kim
A novel acidtolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-staining-negative, catalase- and oxidase- positive, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T assimilates lactic acid, L-proline, and 3-hydroxybenzoic acid; tolerates 4 % of NaCl, ferments glucose, and can grow at pH 11.0. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Selina Poon, Ryan Nixon, Stephen Wendolowski, Rachel Gecelter, Yen Hsun Chen, Jon-Paul DiMauro, Terry Amaral, Adam Graver, Daniel A Grande
PURPOSE: To test for possible thermal injury and tissue damage caused by magnetic-controlled growing rods (MCGRs) during MRI scans. METHODS: Three fresh frozen cadavers were utilized. Four MRI scans were performed: baseline, after spinal hardware implantation, and twice after MCGR implantation. Cross connectors were placed at the proximal end and at the distal end of the construct, making a complete circuit hinged at those two points. Three points were identified as potential sites for significant heating: adjacent to the proximal and distal cross connectors and adjacent to the actuators...
January 9, 2017: European Spine Journal
Cong Sun, Rui-Jun Wang, Yue Su, Ge-Yi Fu, Zhe Zhao, Xiao-Yun Yu, Chong-Ya Zhang, Can Chen, Shuai-Bo Han, Meng-Meng Huang, Zheng-Bing Lv, Min Wu
A Gram-staining-negative, aerobic, non-spore-forming, non-motile, oval to rod shaped bacteria with prosthecate, designated as WM6T, was isolated from seawater sample collected from the South China Sea at depth of 150 meter and subjected to a polyphasic taxonomic investigation. Cells of strain WM6T were approximately 0.5-0.6 μm in width and 0.8-1.2 μm in length, and colonies of strain WM6T were smooth, circular, convex and whity-yellow. Strain WM6T was found to grow at 10-45 °C (optimum, 30 °C), pH 6.5-9...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Inam Ullah Khan, Firasat Hussain, Ye Tian, Neeli Habib, Wen-Dong Xian, Zhao Jiang, Arshia Amin, Chang-Guo Yuan, En-Min Zhou, Xiao-Yang Zhi, Wen-Jun Li
Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008 were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. Taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-staining negative, aerobic, short rod shaped and motile by means of a polar flagellum. They were oxidase and catalase positive and were able to grow at 30-55 °C (optimum, 37-45 °C), pH 6.0-8.0 (optimum, pH 7...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Tomohiro G Noguchi, Yasutaka Iwashita, Yasuyuki Kimura
Amphiphilic Janus particles (AJP), composed of hydrophilic and hydrophobic hemispheres, are one of the simplest anisotropic colloids, and they exhibit higher surface activities than particles with homogeneous surface properties. Consequently, a ternary system of AJP, water and oil can form extremely stable Pickering emulsions, with internal structures that depend on the Janus structure of the particles and the system composition. However, the detail of these structures has not been fully explored, especially for the composition range where the amount of the minority liquid phase and AJP are comparable, where one would expect the Janus characteristics to be directly reflected...
January 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Alicja Sękowska
The genus Raoultella belongs to the family of Enterobacteriaceae. Raoultella spp. are Gram-negative, aerobic, non-motile rods. This genus can be distinguished from the genus Klebsiella, in that genus use histamine as the only source of carbon in the medium. Also, Raoultella grow at 4 °C and do not produce gas from lactose at 44.5 °C. Raoultella sp. is known to inhabit natural environments (water, soil, plants). The reservoir of Raoultella is the gastrointestinal tract and upper respiratory tract. Raoultella spp...
January 6, 2017: Folia Microbiologica
Azadeh Shahinpei, Mohammad Ali Amoozegar, Seyed Abolhassan Shahzadeh Fazeli, Peter Schumann, Cathrin Spröer, Antonio Ventosa
A novel Gram-staining-negative straight or curved rod, moderately halophilic and alkaliphilic bacterium, designated strain GBSy1T, was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Strain GBSy1T was motile, and formed non-pigmented, mucoid colonies. Growth occurred between 1 and 15 % (w/v) NaCl and the isolate grew optimally with 5 % (w/v) NaCl. The optimum pH and temperature for growth were 8.5 and 34 °C, while the strain was able to grow at pH 7.0-10 and 4-40 °C. On the basis of 16S rRNA gene sequence analysis, strain GBSy1T was shown to belong to the genus Aliidiomarina within the class Gammaproteobacteria, family Idiomarinaceae and showed closest phylogenetic similarity to Aliidiomarina maris CF12-14T (97...
January 4, 2017: International Journal of Systematic and Evolutionary Microbiology
Zeinab Jahed, Hamed Shahsavan, Mohit S Verma, Jacob L Rogowski, Brandon B Seo, Boxin Zhao, Ting Y Tsui, Frank X Gu, Mohammad R K Mofrad
Bacteria have evolved as intelligent microorganisms that can colonize and form highly structured and cooperative multicellular communities with sophisticated singular and collective behaviors. The initial stages of colony formation and intercellular communication are particularly important to understand and depend highly on the spatial organization of cells. Controlling the distribution and growth of bacterial cells at the nanoscale is, therefore, of great interest in understanding the mechanisms of cell-cell communication at the initial stages of colony formation...
January 9, 2017: ACS Nano
Umesh Metkar, Swamy Kurra, David Quinzi, Stephen Albanese, William F Lavelle
Early onset scoliosis can be both a disfiguring as well as a life threatening condition. When more conservative treatments fail, pediatric spinal surgeons are forced to consider operative interventions. Traditionally, these interventions have involved the insertion of a variety of implants into the patient with a limited number of anchor points controlling the spine. In the past, these pediatric patients have had multiple surgeries for elective lengthening of these devices to facilitate their growth while attempting to control the scoliosis...
January 3, 2017: Expert Review of Medical Devices
Julie Lebon, Cécile Batailler, Matthieu Wargny, Elie Choufani, Philippe Violas, Damien Fron, Jerry Kieffer, Franck Accadbled, Vincent Cunin, Jérôme Sales De Gauzy
PURPOSE: Preliminary results of magnetically controlled growing rods (MCGR) are encouraging. However, only short case series of MCGR for the treatment of early onset scoliosis (EOS) have been reported. Our aim was to evaluate its effectiveness and complications. METHODS: We report a 30-case retrospective, consecutive, multicenter series of MCGR. Effectiveness was judged upon: deformity correction and difficulties to achieve desired distraction. Secondary endpoints included complications and revision surgeries...
December 31, 2016: European Spine Journal
William P J Smith, Yohan Davit, James M Osborne, Wook Kim, Kevin R Foster, Joe M Pitt-Francis
The clearest phenotypic characteristic of microbial cells is their shape, but we do not understand how cell shape affects the dense communities, known as biofilms, where many microbes live. Here, we use individual-based modeling to systematically vary cell shape and study its impact in simulated communities. We compete cells with different cell morphologies under a range of conditions and ask how shape affects the patterning and evolutionary fitness of cells within a community. Our models predict that cell shape will strongly influence the fate of a cell lineage: we describe a mechanism through which coccal (round) cells rise to the upper surface of a community, leading to a strong spatial structuring that can be critical for fitness...
December 30, 2016: Proceedings of the National Academy of Sciences of the United States of America
Jason Pui Yin Cheung, Cora Bow, Dino Samartzis, Kenny Kwan, Kenneth Man Chee Cheung
PURPOSE: To assess the effect of frequent small distractions with a magnetically controlled growing rod (MCGR) on spinal length gain and achieved distraction length in children with early-onset scoliosis (EOS), and to determine whether the law of diminishing returns applies to this group of patients with MCGR. METHODS: A consecutive series of 3 males and 4 females with EOS who underwent MCGR implantation at a mean age of 10.2 years and were followed up for a mean of 3...
December 2016: Journal of Orthopaedic Surgery
Wankupar Wankhar, Sakthivel Srinivasan, Loganathan Sundareswaran, Dapkupar Wankhar, Ravindran Rajan, Rathinasamy Sheeladevi
Noise pollution is one of the most widespread and fast growing environmental and occupational menaces in the modern era. Exposure to noise above 100dB is not adaptable through the brain homeostatic mechanism. Yet, the detrimental effects of noise have often been ignored. Developing reliable animal models to understand the neurobiology of noise stress and advance our research in the field of medicine to impede this growing stressor is needed. In this study experimental animals were divided into four groups, (i) Control and (ii) S...
December 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Joshua M Pahys, James T Guille
BACKGROUND: Congenital scoliosis is a failure of vertebral formation, segmentation, or a combination of the 2 arising from abnormal vertebral development during weeks 4 to 6 of gestation. The associated spinal deformity can be of varying severity and result in a stable or progressive deformity based on the type and location of the anomalous vertebra(e). Bracing for congenital scoliosis is rarely indicated, while recent reports have demonstrated the utility of serial derotational casting for longer curves with multiple anomalous vertebrae as an effective "time buying strategy" to delay the need for surgery...
December 22, 2016: Journal of Pediatric Orthopedics
Hong-Li Si, Fei-Xiang Shi, Lu-Lu Zhang, Hong-Shan Yue, Hai-Ying Wang, Zun-Tian Zhao
An actinobacterium, designated strain 9583bT, was isolated from the lichen Lobaria retigera collected from Jiaozi Snow Mountain, Yunnan Province, China. Cells of strain 9583bT were Gram-stain-positive, aerobic, catalase-positive and oxidase-negative. The strain have a short rod-shaped, irregular morphology and could grow at the temperature range of 4 to 28 ºC. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain 9583bT belonged to the genus Subtercola in the family Microbacteriaceae, and shared highest sequence similarity with the type strains of Subtercola frigoramans and Subtercola boreus (96...
December 22, 2016: International Journal of Systematic and Evolutionary Microbiology
Dhiraj Kumar Chaudhary, Jaisoo Kim
A straw-coloured, Gram-staining negative, aerobic, motile, and rod shaped bacterium, designated strain K-1-15T, was isolated from reclaimed grassland soil of Biratnagar, Morang, Nepal. This strain was non-spore forming, catalase-negative, and oxidase-positive. It was able to grow at 10-45°C, pH 6.5-9.5, and 0-1.5% (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain K-1-15T formed a distinct lineage within the family Oxalobacteraceae and was most closely related to genera Herbaspirillum (96...
December 20, 2016: International Journal of Systematic and Evolutionary Microbiology
Ye-Ji Hwang, Sa-Youl Ghim
Strain KUDC4121T was isolated from the rhizosphere of Acer okamotoanum, a plant native to the Korean island of Ulleungdo. The strain is a Gram stain-positive, non-spore-forming, non-motile, rod-shaped bacterium that can grow at 18-37 °C and pH 6.0-7.5, with optimum growth at 30 °C and pH 7.0. It grows on tryptic soy agar containing less than 0.5% NaCl (w/v) and in R2A broth. The cell length ranges from 2.0 to 2.5 μm. KUDC4121T is oxidase- and catalase-positive and does not hydrolyse starch or casein. The genomic G+C content is 48...
December 20, 2016: International Journal of Systematic and Evolutionary Microbiology
Nathan D Burrows, Samantha Harvey, Fred A Idesis, Catherine J Murphy
Since the development of simple, aqueous protocols for the synthesis of anisotropic metal nanoparticles, research into many promising, valuable applications of gold nanorods has grown considerably, but a number of challenges remain, including gold-particle yield, robustness to minor impurities, and precise control of gold nanorod surface chemistry. Herein we present the results of a composite fractional factorial series of experiments designed to screen seven additional potential avenues of control and to understand the seed-mediated silver-assisted synthesis of gold nanorods...
December 16, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Elena I Kompantseva, Ilya V Kublanov, Anna A Perevalova, Nikolay A Chernyh, Stepan V Toshchakov, Yuriy V Litti, Alexey N Antipov, Elizaveta A Bonch-Osmolovskaya, Margarita L Miroshnichenko
A moderately thermophilic, anaerobic bacterium designated as strain KRT was isolated from a shallow water submarine hydrothermal vent (Kunashir Island, Southern Kurils, Russia). Cells of the strain KRT were thin (0.2-0.3 µm), flexible, motile, Gram-negative rods of variable length. Optimal growth conditions: pH 6.6, 55°C, 1-3% (w/v) NaCl. Strain KRT was able to ferment a wide range of proteinaceous substrates, pyruvate, mono-, di- and polysaccharides. The best growth occurred with proteinaceous compounds...
December 16, 2016: International Journal of Systematic and Evolutionary Microbiology
Muhammad Zubair Siddiqi, Zubair Aslam, Wan-Taek Im
A Gram-reaction negative, catalase and oxidase positive, aerobic, non-motile, light yellow colored and rod-shaped bacterium (designated Gsoil 809T) isolated from soil of ginseng field, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 809T was observed to grow optimally at 30 ºC and at pH 7.0 on nutrient agar medium. Strain Gsoil 809T possessed β-glucosidase activity, which was responsible for its ability to transform protopanaxatriol type ginsenoside Rg1 to ginsenoside Rh1...
December 14, 2016: International Journal of Systematic and Evolutionary Microbiology
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