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https://www.readbyqxmd.com/read/28187066/learning-curve-in-monitoring-magnetically-controlled-growing-rod-distractions-with-ultrasound
#1
Jason P Y Cheung, Karen K L Yiu, Cora Bow, Prudence W H Cheung, Dino Samartzis, Kenneth M C Cheung
STUDY DESIGN: Prospective study. OBJECTIVE: To determine whether a learning curve exists for ultrasound measurement of magnetically controlled growing rod (MCGR) distractions. SUMMARY OF BACKGROUND DATA: For patients managed by MCGRs, close monitoring of interval distraction length gains is important to determine whether the distractions are translating into actual spine growth. Radiographs are the criterion standard for measuring length gains, but ultrasound has been shown to be effective in monitoring distraction lengths without radiation exposure...
February 9, 2017: Spine
https://www.readbyqxmd.com/read/28186610/-progress-in-research-on-pathogenic-genes-and-gene-therapy-for-inherited-retinal-diseases
#2
Ling Zhu, Cong Cao, Jiji Sun, Tao Gao, Xiaoyang Liang, Zhipeng Nie, Yanchun Ji, Pingping Jiang, Minxin Guan
Inherited retinal diseases (IRDs), including retinitis pigmentosa, Usher syndrome, Cone-Rod degenerations, inherited macular dystrophy, Leber's congenital amaurosis, Leber's hereditary optic neuropathy are the most common and severe types of hereditary ocular diseases. So far more than 200 pathogenic genes have been identified. With the growing knowledge of the genetics and mechanisms of IRDs, a number of gene therapeutic strategies have been developed in the laboratory or even entered clinical trials. Here the progress of IRD research on the pathogenic genes and therapeutic strategies, particularly gene therapy, are reviewed...
February 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28169038/a-growing-need-hiv-education-in-long-term-care
#3
Kaitlin Siou, Maureen Mahan, Rod Cartagena, Soo Chan Carusone
As people living with HIV (PLHIV) age, knowledge of HIV and the associated care of those aging with HIV will become an increasingly important component of education for long-term care (LTC) providers. This descriptive study piloted two different approaches to distribute narrative-based HIV educational videos. Four LTC facilities were assigned to receive the videos to implement 'as usual' or to receive the videos in addition to blended learning sessions where the videos were shown with facilitated discussion with a nurse educator and a PLHIV...
February 3, 2017: Geriatric Nursing
https://www.readbyqxmd.com/read/28163823/draft-genome-sequence-of-type-strain-hbr26-t-and-description-of-rhizobium-aethiopicum-sp-nov
#4
Aregu Amsalu Aserse, Tanja Woyke, Nikos C Kyrpides, William B Whitman, Kristina Lindström
Rhizobium aethiopicum sp. nov. is a newly proposed species within the genus Rhizobium. This species includes six rhizobial strains; which were isolated from root nodules of the legume plant Phaseolus vulgaris growing in soils of Ethiopia. The species fixes nitrogen effectively in symbiosis with the host plant P. vulgaris, and is composed of aerobic, Gram-negative staining, rod-shaped bacteria. The genome of type strain HBR26(T) of R. aethiopicum sp. nov. was one of the rhizobial genomes sequenced as a part of the DOE JGI 2014 Genomic Encyclopedia project designed for soil and plant-associated and newly described type strains...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28141504/luteitalea-pratensis-gen-nov-sp-nov-a-new-member-of-subdivision-6-acidobacteria-isolated-from-temperate-grassland-soil
#5
Selma Vieira, Maja Luckner, Gerhard Wanner, Jorg Overmann
Albeit being widespread and abundant in soils worldwide, bacteria of the phylum Acidobacteria have remained grossly understudied due to difficulties in their cultivation and isolation. To date only 48 species have been validly described, including a single member of the phylogenetically diverse Acidobacteria subdivision 6. Here, we report the polyphasic characterization of strain HEG_-6_39T, a new representative of Acidobacteria subdivision 6 isolated from a grassland soil in Thuringia, Germany. Cells of HEG_-6_39T are Gram-negative, non-motile, non-spore-forming, non-capsulated short rods that form small dark yellow colonies...
January 31, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28135405/the-potential-source-of-b-licheniformis-contamination-during-whey-protein-concentrate-80-manufacture
#6
Siti Norbaizura Md Zain, Rod Bennett, Steve Flint
The objective of this study was to determine the possible source of predominant Bacillus licheniformis contamination in a whey protein concentrate (WPC) 80 manufacturing plant. Traditionally, microbial contaminants of WPC were believed to grow on the membrane surfaces of the ultrafiltration plant as this represents the largest surface area in the plant. Changes from hot to cold ultrafiltration have reduced the growth potential for bacteria on the membrane surfaces. Our recent studies of WPCs have shown the predominant microflora B...
January 30, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28131039/bioleaching-of-electronic-waste-using-bacteria-isolated-from-the-marine-sponge-hymeniacidon-heliophila-porifera
#7
Enrique E Rozas, Maria A Mendes, Claudio A O Nascimento, Denise C R Espinosa, Renato Oliveira, Guilherme Oliveira, Marcio R Custodio
The bacteria isolated from Hymeniacidon heliophila sponge cells showed bioleaching activity. The most active strain, Hyhel-1, identified as Bacillus sp., was selected for bioleaching tests under two different temperatures, 30°C and 40°C, showing rod-shaped cells and filamentous growth, respectively. At 30°C, the bacteria secreted substances which linked to the leached copper, and at 40°C metallic nanoparticles were produced inside the cells. In addition, infrared analysis detected COOH groups and linear peptides in the tested bacteria at both temperatures...
January 22, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28126050/rhodanobacter-rhizosphaerae-sp-nov-isolated-from-soil-of-ginseng-rhizosphere
#8
Geon-Yeong Cho, Jae-Chan Lee, Kyung-Sook Whang
A Gram-stain-negative, aerobic, non-motile, non-spore-forming, yellow and rod-shaped bacterium, designated strain CR164T, was isolated from the rhizosphere soil of a ginseng field at Geumsan in Korea. Strain CR164T grew between 15 and 37 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0-1.0 % (w/v) NaCl, growing optimally in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CR164T belongs to the genus Rhodanobacter, showing the highest sequence similarity to Rhodanobacter caeni MJ01T (98...
January 26, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28126039/rhizobium-arenae-sp-nov-isolated-from-the-sand-of-desert-mu-us-china
#9
Shengnan Zhang, Shanshan Yang, Wei Chen, Yong Chen, Mingjuan Zhang, Xinai Zhou, Guohua Fan, Fu Ying Feng
A Gram-strain-negative, rod-shaped, motile bacterium, designated MIM27T, was isolated from the sand of Desert Mu Us, China. The strain could grow at 4-45 °C (optimum, 37 °C), at pH 6.5-9.0 (optimum, 8.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis on 16S rRNA gene sequence demonstrated that the strain was belonged to the genus Rhizobium, with the highest similarity (96.5 %) to Rhizobium pakistanensis BN-19T. Analysis of nitrogen fixation gene nifH and three housekeeping gene sequences of recA, atpD and glnII were also showed that strain MIM27T was mostly related to the recognized species of the genus Rhizobium but the similarities were low ( 90...
January 23, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28122955/the-vps13p-cdc31p-complex-is-directly-required-for-tgn-late-endosome-transport-and-tgn-homotypic-fusion
#10
Mithu De, Austin N Oleskie, Mariam Ayyash, Somnath Dutta, Liliya Mancour, Mohamed E Abazeed, Eddy J Brace, Georgios Skiniotis, Robert S Fuller
Yeast VPS13 is the founding member of a eukaryotic gene family of growing interest in cell biology and medicine. Mutations in three of four human VPS13 genes cause autosomal recessive neurodegenerative or neurodevelopmental disease, making yeast Vps13p an important structural and functional model. Using cell-free reconstitution with purified Vps13p, we show that Vps13p is directly required both for transport from the trans-Golgi network (TGN) to the late endosome/prevacuolar compartment (PVC) and for TGN homotypic fusion...
February 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28117980/in-situ-photochemical-activation-of-sulfate-for-enhanced-degradation-of-organic-pollutants-in-water
#11
Guoshuai Liu, Shijie You, Yang Tan, Nanqi Ren
The advanced oxidation process (AOP) based on SO4(•-) radicals has been receiving growing attention in water and wastewater treatment. Producing SO4(•-) radicals by activation of peroxymonosulfate or persulfate faces the challenges of high operational cost and potential secondary pollution. In this study, we report the in situ photochemical activation of sulfate (i-PCAS) to produce SO4(•-) radicals with bismuth phosphate (BPO) serving as photocatalyst. The prepared BPO rod-like material could achieve remarkably enhanced degradation of 2,4-dichlorophenol (2,4-DCP) in the presence of sulfate, indicated by the first-order kinetic constant (k = 0...
February 6, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28113034/biomimetic-enamel-regeneration-mediated-by-leucine-rich-amelogenin-peptide
#12
S Y Kwak, A Litman, H C Margolis, Y Yamakoshi, J P Simmer
We report here a novel biomimetic approach to the regeneration of human enamel. The approach combines the use of inorganic pyrophosphate (PPi) to control the onset and rate of enamel regeneration and the use of leucine-rich amelogenin peptide (LRAP), a nonphosphorylated 56-amino acid alternative splice product of amelogenin, to regulate the shape and orientation of growing enamel crystals. This study builds on our previous findings that show LRAP can effectively guide the formation of ordered arrays of needle-like hydroxyapatite (HA) crystals in vitro and on the known role mineralization inhibitors, like PPi, play in the regulation of mineralized tissue formation...
January 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28109202/algoriphagus-resistens-sp-nov-isolated-from-marine-sediment
#13
Ji-Ru Han, Jin-Xin Zhao, Zong-Jie Wang, Guan-Jun Chen, Zong-Jun Du
Strain NH1T, a pink-pigmented, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative, Gram-stain-negative marine bacterium, was isolated from marine sediment in the coast of Weihai, China. Cells of strain NH1T was rod-shaped, 0.8-2.0 µm in length and 0.5 µm in width. The strain able to grow at 13-37 °C, pH 5.5-8.5, in the presence of 0.0-8.0% (w/v) NaCl. Optimal growth was observed at 28 °C, with 3.0% (w/v) NaCl and pH 6.5-7.0. Nitrate was reduced. The G+C content of the DNA was 41...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28107419/a-new-israeli-tobamovirus-isolate-infects-tomato-plants-harboring-tm-22-resistance-genes
#14
Neta Luria, Elisheva Smith, Victoria Reingold, Ilana Bekelman, Moshe Lapidot, Ilan Levin, Nadav Elad, Yehudit Tam, Noa Sela, Ahmad Abu-Ras, Nadav Ezra, Ami Haberman, Liron Yitzhak, Oded Lachman, Aviv Dombrovsky
An outbreak of a new disease infecting tomatoes occurred in October-November 2014 at the Ohad village in Southern Israel. Symptomatic plants showed a mosaic pattern on leaves accompanied occasionally by narrowing of leaves and yellow spotted fruit. The disease spread mechanically and rapidly reminiscent of tobamovirus infection. Epidemiological studies showed the spread of the disease in various growing areas, in the South and towards the Southeast and Northern parts of the country within a year. Transmission electron microscope (TEM) analysis showed a single rod-like form characteristic to the Tobamovirus genus...
2017: PloS One
https://www.readbyqxmd.com/read/28100313/rectinema-cohabitans-gen-nov-sp-nov-a-rod-shaped-spirochete-isolated-from-an-anaerobic-naphthalene-degrading-enrichment-culture
#15
Janina S Koelschbach, Housna Mouttaki, Carolin Pickl, Hermann J Heipieper, Reinhard Rachel, Paul A Lawson, Rainer U Meckenstock
The anaerobic, non-motile strain HMT was isolated from the naphthalene-degrading, sulfate-reducing enrichment culture N47. Since 20 years, strain HMT has been a stable member of culture N47 although it is neither able to degrade naphthalene nor to reduce sulfate in pure culture. The highest similarity of the 16S rRNA gene sequence of strain HMT (89%) is with a cultivated member of the family Spirochaetaceae, Treponema caldarium sp. strain H1T (DSM 7334T), an obligately anaerobic, thermophilic spirochete isolated from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA...
January 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28097251/management-of-progressive-late-onset-scoliosis-with-magnetic-growth-rod-insertion-leading-to-improvement-of-neural-anomalies-a-case-report
#16
Amit Zaveri, Valerio Pace, Dimpu Bhagawati, Vijay Rajamani, Thillainayagam Muthukumar, Hilali Noordeen
BACKGROUND: To present the first known reported case of late onset idiopathic scoliosis with concomitant neural anomalies, treated with sequential distraction using magnetic growth rod, had significant improvement in both cranio-cervical and intraspinal anomaly. METHODS: A caucasian, growing female child (at the age of ten) presented with moderately progressive late onset right thoracic scoliosis. She was found to have Chiari type I malformation and a cervicothoracic syrinx on routine pre-operative MRI scanning...
December 2016: Journal of Spine Surgery (Hong Kong)
https://www.readbyqxmd.com/read/28073405/rhodanobacter-humi-sp-nov-a-novel-acidtolerant-and-alkalitolerant-gammaproteobacterium-isolated-from-kyonggi-university-forest-soil
#17
Ram Hari Dahal, Jaisoo Kim
A novel acidtolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-staining-negative, catalase- and oxidase- positive, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T assimilates lactic acid, L-proline, and 3-hydroxybenzoic acid; tolerates 4 % of NaCl, ferments glucose, and can grow at pH 11.0. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28070684/a-pilot-cadaveric-study-of-temperature-and-adjacent-tissue-changes-after-exposure-of-magnetic-controlled-growing-rods-to-mri
#18
Selina Poon, Ryan Nixon, Stephen Wendolowski, Rachel Gecelter, Yen Hsun Chen, Jon-Paul DiMauro, Terry Amaral, Adam Graver, Daniel A Grande
PURPOSE: To test for possible thermal injury and tissue damage caused by magnetic-controlled growing rods (MCGRs) during MRI scans. METHODS: Three fresh frozen cadavers were utilized. Four MRI scans were performed: baseline, after spinal hardware implantation, and twice after MCGR implantation. Cross connectors were placed at the proximal end and at the distal end of the construct, making a complete circuit hinged at those two points. Three points were identified as potential sites for significant heating: adjacent to the proximal and distal cross connectors and adjacent to the actuators...
January 9, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28068219/hyphobacterium-vulgare-gen-nov-sp-nov-a-novel-alpha-proteobacterium-isolated-from-seawater-in-the-south-china-sea
#19
Cong Sun, Rui-Jun Wang, Yue Su, Ge-Yi Fu, Zhe Zhao, Xiao-Yun Yu, Chong-Ya Zhang, Can Chen, Shuai-Bo Han, Meng-Meng Huang, Zheng-Bing Lv, Min Wu
A Gram-staining-negative, aerobic, non-spore-forming, non-motile, oval to rod shaped bacteria with prosthecate, designated as WM6T, was isolated from seawater sample collected from the South China Sea at depth of 150 meter and subjected to a polyphasic taxonomic investigation. Cells of strain WM6T were approximately 0.5-0.6 μm in width and 0.8-1.2 μm in length, and colonies of strain WM6T were smooth, circular, convex and whity-yellow. Strain WM6T was found to grow at 10-45 °C (optimum, 30 °C), pH 6.5-9...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28068215/tibeticola-sediminis-gen-nov-sp-nov-a-thermophilic-bacterium-isolated-from-hot-spring
#20
Inam Ullah Khan, Firasat Hussain, Ye Tian, Neeli Habib, Wen-Dong Xian, Zhao Jiang, Arshia Amin, Chang-Guo Yuan, En-Min Zhou, Xiao-Yang Zhi, Wen-Jun Li
Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008 were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. Taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-staining negative, aerobic, short rod shaped and motile by means of a polar flagellum. They were oxidase and catalase positive and were able to grow at 30-55 °C (optimum, 37-45 °C), pH 6.0-8.0 (optimum, pH 7...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
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