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Pecs I y Ii

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https://www.readbyqxmd.com/read/11789603/comparison-of-the-accessory-activity-of-murine-peritoneal-cavity-macrophage-derived-dendritic-cells-and-peritoneal-cavity-macrophages-in-a-mixed-lymphocyte-reaction
#1
COMPARATIVE STUDY
L H Makala, Y Nishikawa, T Kamada, H Suzuki, X Xuan, I Igarashi, H Nagasaw
A detailed comparison of the accessory cell activities was carried out among murine peritoneal cavity macrophages (PEC-Mphi), peritonea] cavity macrophages stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4), the most popular cytokine combination widely used to generate dendritic cells (DC) and peritoneal cavity macrophage-derived DC (PEC-DC) using a two-way mixed lymphocyte reaction (MLR). All the cell types used efficiently induced statistically significant naïve T cell proliferation at all culture time points and responder:stimulator ratios used...
December 2001: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/11082111/lack-of-involvement-of-endothelin-1-in-angiotensin-ii-induced-contraction-of-the-isolated-rat-tail-artery
#2
Y Jiang, C R Triggle
1. The contribution of endothelin-1 (ET-1) to angiotensin II (Ang II)-mediated contraction of the isolated rat tail artery was assessed with measurements of tension, and cytosolic calcium ([Ca(2+)](i)). The distribution of the AT(1) receptor was studied with RT - PCR and immunohistochemistry. 2. Ang II induced an endothelium-independent contraction (pEC(50) 7.95+/-0.06 and E(max): 0.46 g+/-0.05 with endothelium vs 7.81+/-0.02 and 0.41 g+/-0.07 without endothelium; P>0.05). Ang II (0.003 - 0.3 microM)-induced a non-sustained contraction of endothelium-intact preparations which was not antagonized by BQ-123 (1 microM), but was inhibited by losartan (10 nM)...
November 2000: British Journal of Pharmacology
https://www.readbyqxmd.com/read/10368657/down-regulation-of-il-10-enhances-the-efficacy-of-locoregional-immunotherapy-using-ok-432-against-malignant-effusion
#3
J Hihara, Y Yamaguchi, K Minami, K Noma, T Toge
To determine the significance of interleukin (IL)-10 in antitumor immune response, the effect of the down-regulation of tumor-derived IL-10 on locoregional immunotherapy was investigated. C3H/HeN mice were intraperitoneally (i.p.) inoculated with IL-10-producing murine breast cancer cell line, FM3A, and treated with locoregional administration of OK-432 with or without anti-IL-10 monoclonal antibody (mAb). Anti-IL-10 mAb did not affect the in vitro growth of FM3A cells. Administration of OK-432 plus anti-IL-10 mAb remarkably delayed the retention of malignant ascites and prolonged the survival of mice compared with the administration of OK-432 alone...
March 1999: Anticancer Research
https://www.readbyqxmd.com/read/8679675/two-amine-oxidases-from-aspergillus-niger-aku-3302-contain-topa-quinone-as-the-cofactor-unusual-cofactor-link-to-the-glutamyl-residue-occurs-only-at-one-of-the-enzymes
#4
I Frébort, P Pec, L Luhová, H Toyama, K Matsushita, S Hirota, T Kitagawa, T Ueno, Y Asano, Y Kato, O Adachi
Amine oxidases (EC 1.4.3.6) from Aspergillus niger, AO-I (2 x 75 kDa) and AO-II (80 kDa), were examined to determine the cofactor structure. Inactivated with p-nitrophenylhydrazine, they showed absorption and fluorescence spectra similar to those published for other copper amine oxidases and to topa hydantoin p-nitrophenylhydrazone. After digestion by thermolysin and pronase, cofactor peptides were purified by HPLC and sequenced. For thermolytic peptides, a typical topa consensus sequence, Asn-X-Glu-Tyr, was obtained for AO-II, although in case of AO-I it overlapped with Val-Val-Ile-Glu-Pro-Tyr-Gly...
June 7, 1996: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/8620882/two-distinct-quinoprotein-amine-oxidases-are-induced-by-n-butylamine-in-the-mycelia-of-aspergillus-niger-aku-3302-purification-characterization-cdna-cloning-and-sequencing
#5
I Frébort, H Tamaki, H Ishida, P Pec, L Luhová, H Tsuno, M Halata, Y Asano, Y Kato, K Matsushita, H Toyama, H Kumagai, O Adachi
Two distinct quinoprotein amine oxidases were found in Aspergillus niger mycelia grown on n-butylamine medium and purified using chromatographic techniques. The respective enzymes were termed AO-I, which had already been isolated, and AO-II, a new enzyme found in this study. HPLC indicated that their molecular masses are 150 kDa and 80 kDa, respectively. On SDS/PAGE, the enzymes gave a similar but distinct mobility, which corresponds to 75 kDa for the subunit dimeric AO-I and 80 kDa for monomeric AO-II. The absorption spectra of both enzymes were different from each other; the absorption maxima in the visible region were at 490 nm for AO-I and 420 nm for AO-II...
April 1, 1996: European Journal of Biochemistry
https://www.readbyqxmd.com/read/8522359/anti-metastatic-activity-induced-by-the-in-vivo-activation-of-purified-protein-derivative-ppd-recognizing-th1-type-cd4-t-cells
#6
Y Shinomiya, M Harada, S Kurosawa, T Okamoto, H Terao, G Matsuzaki, T Shirakusa, K Nomoto
The Th1 type Cd4+ T cell clone (MH2), which is capable of recognizing purified protein derivative from Mycobacterium tuberculosis (PPD), was examined for its anti-metastatic activity against melanoma. In using an in vitro proliferative assay, MH2 was able to recognize PPD-derived antigen in a major histocompatibility complex class II-restricted manner. MH2 showed neither any natural killer (NK) activity nor cytolytic activity against syngeneic B16 melanoma. This clone produced interferon-gamma, tumor necrosis factor and interleukin-2, but not interleukin-4, when co-cultured with PPD and irradiated syngeneic C57BL/6 spleen cells, suggesting that this clone could thus be assigned to the Th1 subset...
August 1995: Immunobiology
https://www.readbyqxmd.com/read/8228216/evidence-that-peritoneal-exudate-cells-cultured-with-eye-derived-fluids-are-the-proximate-antigen-presenting-cells-in-immune-deviation-of-the-ocular-type
#7
Y Hara, S Okamoto, B Rouse, J W Streilein
Immune privilege in the anterior chamber of the eye results in part from a selective deficit in delayed hypersensitivity that is elicited by antigenic materials placed in this unique tissue site. This distinctive systemic immune response to intraocular Ag (termed anterior chamber-associated immune deviation, ACAID) is fashioned by indigenous, intraocular bone marrow-derived cells that capture Ag within the anterior chamber and carry an Ag-specific ACAID-inducing signal via the blood directly to the spleen. An identical form of immune deviation can be evoked by the i...
November 15, 1993: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/7871568/induction-of-anterior-chamber-associated-immune-deviation-with-lymphoreticular-allogeneic-cells
#8
S Okamoto, Y Hara, J W Streilein
Immune privilege in the anterior chamber of the eye results in part from a selective deficit in delayed hypersensitivity that is elicited by antigenic materials placed in this unique tissue site. This stereo-typical systemic immune response (anterior chamber-associated immune deviation [ACAID]) to intraocular antigen can also be evoked in naive mice by the intravenous injection of syngeneic peritoneal exudate cells (PEC) that have been incubated overnight with a soluble antigen in the presence of supernatants of cultured iris and ciliary body (I/CB) cells or with transforming growth factor (TGF)-beta...
February 15, 1995: Transplantation
https://www.readbyqxmd.com/read/2379941/studies-on-the-immunological-properties-of-oxysterols-in-vivo-actions-of-7-25-dihydroxycholesterol-upon-murine-peritoneal-cells
#9
C Moog, Y H Ji, C Waltzinger, B Luu, P Bischoff
Oxygenated derivatives of cholesterol are potent immunosuppressors. It has been reported previously that 7,25-dihydroxycholesterol (7,25-OHC), synthesized in the URA31, strongly inhibits the early steps of T-cell activation. So far, the mechanisms underlying this type of effect have been mainly investigated in vitro, and the activity of these substances on the immune system has been poorly studied. This study describes that a single i.p. injection of 7,25-OHC induces a strong inflammatory response, consisting of a massive influx of macrophages and neutrophils into the abdominal cavity...
July 1990: Immunology
https://www.readbyqxmd.com/read/1721041/intrathymic-induction-of-neonatal-tolerance-to-mls-1a-determinant-clonal-deletion-and-clonal-anergy-by-haematolymphoid-cells
#10
S Ideyama, M Hosono, S Imamura, M Tomana, Y Katsura
Newborn BALB/c (Mls-1b) mice were intravenously injected with either bone marrow cells (BMC) or peritoneal exudate cells (PEC) from Mls semi-allogeneic (BALB/c x AKR)F1 mice. Thymic cells of these mice, obtained 7 days after the injection, were found to be unresponsive to the superantigen Mls-1a, as determined by graft-versus-host reactivity. On Day 7, deletion of T cells expressing the V beta 6 element at high levels (V beta 6hi) was observed in thymic cells of mice receiving PEC. In mice given BMC, it took 2 weeks until the proportion of V beta 6hi T cells began to decline, and a longer period was required for complete disappearance of V beta 6hi T cells...
October 1991: Immunology
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