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https://www.readbyqxmd.com/read/29215563/response-of-ustilago-maydis-against-the-stress-caused-by-three-polycationic-chitin-derivatives
#1
Dario Rafael Olicón-Hernández, Cristina Uribe-Alvarez, Salvador Uribe-Carvajal, Juan Pablo Pardo, Guadalupe Guerra-Sánchez
Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg·mL-1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium...
December 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29212471/investigation-on-the-differentiation-of-two-ustilago-esculenta-strains-implications-of-a-relationship-with-the-host-phenotypes-appearing-in-the-fields
#2
Yafen Zhang, Qianchao Cao, Peng Hu, Haifeng Cui, Xiaoping Yu, Zihong Ye
BACKGROUND: Ustilago esculenta, a pathogenic basidiomycete fungus, infects Zizania latifolia to form edible galls named Jiaobai in China. The distinct growth conditions of U. esculenta induced Z. latifolia to form three different phenotypes, named male Jiaobai, grey Jiaobai and white Jiaobai. The aim of this study is to characterize the genetic and morphological differences that distinguish the two U. esculenta strains. RESULTS: In this study, sexually compatible haploid sporidia UeT14/UeT55 from grey Jiaobai (T strains) and UeMT10/UeMT46 from white Jiaobai (MT strains) were isolated...
December 6, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29209508/promoters-from-the-itaconate-cluster-of-ustilago-maydis-are-induced-by-nitrogen-depletion
#3
Thiemo Zambanini, Sandra K Hartmann, Lisa M Schmitz, Linda Büttner, Hamed Hosseinpour Tehrani, Elena Geiser, Melanie Beudels, Dominik Venc, Georg Wandrey, Jochen Büchs, Markus Schwarzländer, Lars M Blank, Nick Wierckx
Background: Ustilago maydis is known for its natural potential to produce a broad range of valuable chemicals, such as itaconate, from both industrial carbon waste streams and renewable biomass. Production of itaconate, and many other secondary metabolites, is induced by nitrogen limitation in U. maydis. The clustered genes responsible for itaconate production have recently been identified, enabling the development of new expression tools that are compatible with biotechnological processes...
2017: Fungal Biology and Biotechnology
https://www.readbyqxmd.com/read/29177184/overrepresentation-analyses-of-differentially-expressed-genes-in-the-smut-fungus-ustilago-bromivora-during-saprophytic-and-in-planta-growth
#4
Angelika Czedik-Eysenberg, Franziska Rabe, Heinz Ekker, Carmen Czepe, Armin Djamei
We have established the Ustilago bromivora-Brachypodium spp. interaction as a new model pathosystem for biotrophic fungal plant infections of the head smut type (Rabe et al., 2016). In this protocol, the methodology used for comparing gene expression between saprophytic and in planta growth of the fungus is described. The experimental and analytical pipeline, how next generation RNA sequencing (Illumina RNA-Seq) analysis can be used to obtain lists of genes significantly up or down regulated in planta in comparison to axenic culture is given...
August 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/29142828/metabolic-engineering-of-ustilago-trichophora-tz1-for-improved-malic-acid-production
#5
Thiemo Zambanini, Hamed Hosseinpour Tehrani, Elena Geiser, Christiane K Sonntag, Joerg M Buescher, Guido Meurer, Nick Wierckx, Lars M Blank
Ustilago trichophora RK089 has been found recently as a good natural malic acid producer from glycerol. This strain has previously undergone adaptive laboratory evolution for enhanced substrate uptake rate resulting in the strain U. trichophora TZ1. Medium optimization and investigation of process parameters enabled titers and rates that are able to compete with those of organisms overexpressing major parts of the underlying metabolic pathways. Metabolic engineering can likely further increase the efficiency of malate production by this organism, provided that basic genetic tools and methods can be established for this rarely used and relatively obscure species...
June 2017: Metabolic Engineering Communications
https://www.readbyqxmd.com/read/29130207/rna-live-imaging-in-the-model-microorganism-ustilago-maydis
#6
Sabrina Zander, Kira Müntjes, Michael Feldbrügge
An essential feature of protein expression is the tight regulation of when and where a protein is translated from its cognate mRNA. This spatiotemporal expression is particularly important in guaranteeing the correct and efficient targeting of proteins to defined subcellular sites. In order to achieve local translation, mRNAs must be deposited at specific locations. A common mechanism is the active transport of mRNAs along the actin or microtubule cytoskeleton. To study such dynamic transport processes in vivo RNA live imaging is the method of choice...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29076026/rna-seq-analysis-provides-insight-into-reprogramming-of-culm-development-in-zizania-latifolia-induced-by-ustilago-esculenta
#7
Zhi-Dan Wang, Ning Yan, Zheng-Hong Wang, Xiao-Huan Zhang, Jing-Ze Zhang, Hui-Min Xue, Li-Xia Wang, Qi Zhan, Ying-Ping Xu, De-Ping Guo
We report a transcriptome assembly and expression profiles from RNA-Seq data and identify genes responsible for culm gall formation in Zizania latifolia induced by Ustilago esculenta. The smut fungus Ustilago esculenta can induce culm gall in Zizania latifolia, which is used as a vegetable in Asian countries. However, the underlying molecular mechanism of culm gall formation is still unclear. To characterize the processes underlying this host-fungus association, we performed transcriptomic and expression profiling analyses of culms from Z...
October 26, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29052918/contributions-of-recombination-and-repair-proteins-to-telomere-maintenance-in-telomerase-positive-and-negative-ustilago-maydis
#8
Eun Young Yu, Min Hsu, William K Holloman, Neal F Lue
Homologous recombination and repair factors are known to promote both telomere replication and recombination-based telomere extension. Herein we address the diverse contributions of several recombination/repair proteins to telomere maintenance in Ustilago maydis, a fungus that bears strong resemblance to mammals with respect to telomere regulation and recombination mechanisms. In telomerase-positive U. maydis, deletion of rad51 and blm separately caused shortened but stably maintained telomeres, whereas deletion of both engendered similar telomere loss, suggesting that the repair proteins help to resolve similar problems in telomere replication...
October 20, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29042629/development-of-a-real-time-fluorescence-loop-mediated-isothermal-amplification-assay-for-rapid-and-quantitative-detection-of-ustilago-maydis
#9
Yanyong Cao, Lifeng Wang, Liping Duan, Jingjing Li, Juan Ma, Shuna Xie, Lei Shi, Huiyong Li
The common smut of corn, caused by Ustilago maydis is a troublesome disease of maize. Early and accurate detection of U. maydis is essential for the disease management. In this study, primer set Pep-2 was selected for LAMP (loop-mediated isothermal amplification) from 12 sets of primers targeting three U. maydis effector genes See1, Pit2 and Pep1 according to primer screening. The optimal concentrations of Bst DNA polymerase and Mg(2+) as well as inner/outer primer ratio of the LAMP reaction system were screened by combining a single factor experiment and an orthogonal design arrangement...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28994236/mrna-transport-in-fungal-top-models
#10
REVIEW
Dierk Niessing, Ralf-Peter Jansen, Thomas Pohlmann, Michael Feldbrügge
Eukaryotic cells rely on the precise determination of when and where proteins are synthesized. Spatiotemporal expression is supported by localization of mRNAs to specific subcellular sites and their subsequent local translation. This holds true for somatic cells as well as for oocytes and embryos. Most commonly, mRNA localization is achieved by active transport of the molecules along the actin or microtubule cytoskeleton. Key factors are molecular motors, adaptors, and RNA-binding proteins that recognize defined sequences or structures in cargo mRNAs...
October 10, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/28992048/comparative-whole-genome-analysis-reveals-artificial-selection-effects-on-ustilago-esculenta-genome
#11
Zihong Ye, Yao Pan, Yafen Zhang, Haifeng Cui, Gulei Jin, Alice C McHardy, Longjiang Fan, Xiaoping Yu
Ustilago esculenta, infects Zizania latifolia, and induced host stem swollen to be a popular vegetable called Jiaobai in China. It is the long-standing artificial selection that maximizes the occurrence of favourable Jiaobai, and thus maintaining the plant-fungi interaction and modulating the fungus evolving from plant pathogen to entophyte. In this study, whole genome of U. esculenta was sequenced and transcriptomes of the fungi and its host were analysed. The 20.2 Mb U. esculenta draft genome of 6,654 predicted genes including mating, primary metabolism, secreted proteins, shared a high similarity to related Smut fungi...
July 19, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28943887/mechanisms-of-cytokinesis-in-basidiomycetous-yeasts
#12
Sophie Altamirano, Srikripa Chandrasekaran, Lukasz Kozubowski
While mechanisms of cytokinesis exhibit considerable plasticity, it is difficult to precisely define the level of conservation of this essential part of cell division in fungi, as majority of our knowledge is based on ascomycetous yeasts. However, in the last decade more details have been uncovered regarding cytokinesis in the second largest fungal phylum, basidiomycetes, specifically in two yeasts, Cryptococcus neoformans and Ustilago maydis. Based on these findings, and current sequenced genomes, we summarize cytokinesis in basidiomycetous yeasts, indicating features that may be unique to this phylum, species-specific characteristics, as well as mechanisms that may be common to all eukaryotes...
March 2017: Fungal Biology Reviews
https://www.readbyqxmd.com/read/28941233/induction-of-apoptosis-like-cell-death-and-clearance-of-stress-induced-intracellular-protein-aggregates-dual-roles-for-ustilago-maydis-metacaspase-mca1
#13
Dibya Mukherjee, Sayandeep Gupta, N Saran, Rahul Datta, Anupama Ghosh
Metacaspases primarily associate with induction and execution of programmed cell death in protozoa, fungi and plants. In the recent past, several studies have also demonstrated cellular functions of metacaspases other than cell death in different organisms including yeast and protozoa. This study shows similar dual function for the only metacaspase of a biotrophic phytopathogen, Ustilago maydis. In addition to a conventional role in the induction of cell death, Mca1 has been demonstrated to play a key role in maintaining the quality of the cellular proteome...
September 23, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28919501/structural-and-kinetics-characterization-of-the-f1f0-atp-synthase-dimer-new-repercussion-of-monomer-monomer-contact
#14
Mercedes Esparza-Perusquía, Sofía Olvera-Sánchez, Juan Pablo Pardo, Guillermo Mendoza-Hernández, Federico Martínez, Oscar Flores-Herrera
Ustilago maydis is an aerobic basidiomycete that fully depends on oxidative phosphorylation for its supply of ATP, pointing to mitochondria as a key player in the energy metabolism of this organism. Mitochondrial F1F0-ATP synthase occurs in supramolecular structures. In this work, we isolated the monomer (640kDa) and the dimer (1280kDa) and characterized their subunit composition and kinetics of ATP hydrolysis. Mass spectrometry revealed that dimerizing subunits e and g were present in the dimer but not in the monomer...
December 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28904417/itaconic-acid-production-by-filamentous-fungi-in-starch-rich-industrial-residues
#15
Richa Bafana, Sarvanadevi Sivanesan, R A Pandey
Several fungi and starch-rich industrial residues were screened for itaconic acid (IA) production. Out of 15 strains, only three fungal strains were found to produce IA, which was confirmed by HPLC and GC-MS analysis. These strains were identified as Aspergillus terreus strains C1 and C2, and Ustilago maydis strain C3 by sequencing of 18S rRNA gene and internal transcribed spacer regions. Cis-aconitate decarboxylase (cad) gene, which encodes a key enzyme in IA production in A. terreus, was characterized from strains C1 and C2...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28818040/in-silico-prediction-and-characterization-of-secondary-metabolite-biosynthetic-gene-clusters-in-the-wheat-pathogen-zymoseptoria-tritici
#16
Timothy Cairns, Vera Meyer
BACKGROUND: Fungal pathogens of plants produce diverse repertoires of secondary metabolites, which have functions ranging from iron acquisition, defense against immune perturbation, to toxic assaults on the host. The wheat pathogen Zymoseptoria tritici causes Septoria tritici blotch, a foliar disease which is a significant threat to global food security. Currently, there is limited knowledge of the secondary metabolite arsenal produced by Z. tritici, which significantly restricts mechanistic understanding of infection...
August 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28781989/isolation-of-ustilago-bromivora-strains-from-infected-spikelets-through-spore-recovery-and-germination
#17
Jason Bosch, Armin Djamei
Ustilago bromivora is a biotrophic smut fungus infecting Brachypodium sp. It is closely related to the barley-infecting smut Ustilago hordei, and related to the well-studied, gall-inducing model pathogen Ustilago maydis. Upon flowering, the spikelets of U. bromivora-infected plants are filled with black fungal spores. While it is possible to directly use this spore material to infect Brachypodium seeds, in many cases it is more useful to isolate individual strains of U. bromivora for a genetically homogenous population...
July 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28747366/cross-reacting-ustilago-maydis-causing-false-positive-cryptococcal-antigen-test-results
#18
LETTER
Matthew P Cheng, Tien T Nguyen, Leighanne O Parkes, Philippe J Dufresne, Donald C Sheppard
No abstract text is available yet for this article.
October 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28686341/three-regulators-of-g-protein-signaling-differentially-affect-mating-morphology-and-virulence-in-the-smut-fungus-ustilago-maydis
#19
Marino Moretti, Lei Wang, Pierre Grognet, Daniel Lanver, Hannes Link, Regine Kahmann
Regulators of G protein signaling (RGS) proteins modulate heterotrimeric G protein signaling negatively. To broaden an understanding of the roles of RGS proteins in fungal pathogens, we functionally characterized the three RGS protein-encoding genes (rgs1, rgs2 and rgs3) in the phytopathogenic fungus Ustilago maydis. It was found that RGS proteins played distinct roles in the regulation of development and virulence. rgs1 had a minor role in virulence when deleted in a solopathogenic strain. In crosses, rgs1 was dispensable for mating and filamentation, but was required for teliospore production...
July 7, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28616972/dss1-regulates-association-of-brh2-with-rad51
#20
Qingwen Zhou, William K Holloman
Brh2, the BRCA2 ortholog in the fungus Ustilago maydis, mediates delivery of Rad51 to DNA during the course of homology-directed DNA repair. Rad51 interacts with Brh2 through the highly conserved BRC element and through a second region termed CRE located at the extreme carboxy terminus. Dss1, a small intrinsically unstructured protein that interacts with Brh2, is crucial for its activity in DNA repair, but the mechanism of this regulation is uncertain. In previous studies, we found that interaction of Brh2 with DNA was strongly modulated by association with Dss1...
July 5, 2017: Biochemistry
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