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https://www.readbyqxmd.com/read/29042629/development-of-a-real-time-fluorescence-loop-mediated-isothermal-amplification-assay-for-rapid-and-quantitative-detection-of-ustilago-maydis
#1
Yanyong Cao, Lifeng Wang, Liping Duan, Jingjing Li, Juan Ma, Shuna Xie, Lei Shi, Huiyong Li
The common smut of corn, caused by Ustilago maydis is a troublesome disease of maize. Early and accurate detection of U. maydis is essential for the disease management. In this study, primer set Pep-2 was selected for LAMP (loop-mediated isothermal amplification) from 12 sets of primers targeting three U. maydis effector genes See1, Pit2 and Pep1 according to primer screening. The optimal concentrations of Bst DNA polymerase and Mg(2+) as well as inner/outer primer ratio of the LAMP reaction system were screened by combining a single factor experiment and an orthogonal design arrangement...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28994236/mrna-transport-in-fungal-top-models
#2
REVIEW
Dierk Niessing, Ralf-Peter Jansen, Thomas Pohlmann, Michael Feldbrügge
Eukaryotic cells rely on the precise determination of when and where proteins are synthesized. Spatiotemporal expression is supported by localization of mRNAs to specific subcellular sites and their subsequent local translation. This holds true for somatic cells as well as for oocytes and embryos. Most commonly, mRNA localization is achieved by active transport of the molecules along the actin or microtubule cytoskeleton. Key factors are molecular motors, adaptors, and RNA-binding proteins that recognize defined sequences or structures in cargo mRNAs...
October 10, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/28992048/comparative-whole-genome-analysis-reveals-artificial-selection-effects-on-ustilago-esculenta-genome
#3
Zihong Ye, Yao Pan, Yafen Zhang, Haifeng Cui, Gulei Jin, Alice C McHardy, Longjiang Fan, Xiaoping Yu
Ustilago esculenta, infects Zizania latifolia, and induced host stem swollen to be a popular vegetable called Jiaobai in China. It is the long-standing artificial selection that maximizes the occurrence of favourable Jiaobai, and thus maintaining the plant-fungi interaction and modulating the fungus evolving from plant pathogen to entophyte. In this study, whole genome of U. esculenta was sequenced and transcriptomes of the fungi and its host were analysed. The 20.2 Mb U. esculenta draft genome of 6,654 predicted genes including mating, primary metabolism, secreted proteins, shared a high similarity to related Smut fungi...
July 19, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28943887/mechanisms-of-cytokinesis-in-basidiomycetous-yeasts
#4
Sophie Altamirano, Srikripa Chandrasekaran, Lukasz Kozubowski
While mechanisms of cytokinesis exhibit considerable plasticity, it is difficult to precisely define the level of conservation of this essential part of cell division in fungi, as majority of our knowledge is based on ascomycetous yeasts. However, in the last decade more details have been uncovered regarding cytokinesis in the second largest fungal phylum, basidiomycetes, specifically in two yeasts, Cryptococcus neoformans and Ustilago maydis. Based on these findings, and current sequenced genomes, we summarize cytokinesis in basidiomycetous yeasts, indicating features that may be unique to this phylum, species-specific characteristics, as well as mechanisms that may be common to all eukaryotes...
March 2017: Fungal Biology Reviews
https://www.readbyqxmd.com/read/28941233/induction-of-apoptosis-like-cell-death-and-clearance-of-stress-induced-intracellular-protein-aggregates-dual-roles-for-ustilago-maydis-metacaspase-mca1
#5
Dibya Mukherjee, Sayandeep Gupta, Saran N, Rahul Datta, Anupama Ghosh
Metacaspases primarily associate with induction and execution of programmed cell death in protozoa, fungi, and plants. In the recent past, several studies have also demonstrated cellular functions of metacaspases other than cell death in different organisms including yeast and protozoa. The present study shows similar dual function for the only metacaspase of a biotrophic phytopathogen, Ustilago maydis. In addition to a conventional role in the induction of cell death, Mca1 has been demonstrated to play a key role in maintaining the quality of the cellular proteome...
September 23, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28919501/structural-and-kinetics-characterization-of-the-f1f0-atp-synthase-dimer-new-repercussion-of-monomer-monomer-contact
#6
Mercedes Esparza-Perusquía, Sofía Olvera-Sánchez, Juan Pablo Pardo, Guillermo Mendoza-Hernández, Federico Martínez, Oscar Flores-Herrera
Ustilago maydis is an aerobic basidiomycete that fully depends on oxidative phosphorylation for its supply of ATP, pointing to mitochondria as a key player in the energy metabolism of this organism. Mitochondrial F1F0-ATP synthase occurs in supramolecular structures. In this work, we isolated the monomer (640kDa) and the dimer (1280kDa) and characterized their subunit composition and kinetics of ATP hydrolysis. Mass spectrometry revealed that dimerizing subunits e and g were present in the dimer but not in the monomer...
December 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28904417/itaconic-acid-production-by-filamentous-fungi-in-starch-rich-industrial-residues
#7
Richa Bafana, Sarvanadevi Sivanesan, R A Pandey
Several fungi and starch-rich industrial residues were screened for itaconic acid (IA) production. Out of 15 strains, only three fungal strains were found to produce IA, which was confirmed by HPLC and GC-MS analysis. These strains were identified as Aspergillus terreus strains C1 and C2, and Ustilago maydis strain C3 by sequencing of 18S rRNA gene and internal transcribed spacer regions. Cis-aconitate decarboxylase (cad) gene, which encodes a key enzyme in IA production in A. terreus, was characterized from strains C1 and C2...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28818040/in-silico-prediction-and-characterization-of-secondary-metabolite-biosynthetic-gene-clusters-in-the-wheat-pathogen-zymoseptoria-tritici
#8
Timothy Cairns, Vera Meyer
BACKGROUND: Fungal pathogens of plants produce diverse repertoires of secondary metabolites, which have functions ranging from iron acquisition, defense against immune perturbation, to toxic assaults on the host. The wheat pathogen Zymoseptoria tritici causes Septoria tritici blotch, a foliar disease which is a significant threat to global food security. Currently, there is limited knowledge of the secondary metabolite arsenal produced by Z. tritici, which significantly restricts mechanistic understanding of infection...
August 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28781989/isolation-of-ustilago-bromivora-strains-from-infected-spikelets-through-spore-recovery-and-germination
#9
Jason Bosch, Armin Djamei
Ustilago bromivora is a biotrophic smut fungus infecting Brachypodium sp. It is closely related to the barley-infecting smut Ustilago hordei, and related to the well-studied, gall-inducing model pathogen Ustilago maydis. Upon flowering, the spikelets of U. bromivora-infected plants are filled with black fungal spores. While it is possible to directly use this spore material to infect Brachypodium seeds, in many cases it is more useful to isolate individual strains of U. bromivora for a genetically homogenous population...
July 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28747366/cross-reacting-ustilago-maydis-causing-false-positive-cryptococcal-antigen-test-results
#10
LETTER
Matthew P Cheng, Tien T Nguyen, Leighanne O Parkes, Philippe J Dufresne, Donald C Sheppard
No abstract text is available yet for this article.
October 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28686341/three-regulators-of-g-protein-signaling-differentially-affect-mating-morphology-and-virulence-in-the-smut-fungus-ustilago-maydis
#11
Marino Moretti, Lei Wang, Pierre Grognet, Daniel Lanver, Hannes Link, Regine Kahmann
Regulators of G protein signaling (RGS) proteins modulate heterotrimeric G protein signaling negatively. To broaden an understanding of the roles of RGS proteins in fungal pathogens, we functionally characterized the three RGS protein-encoding genes (rgs1, rgs2 and rgs3) in the phytopathogenic fungus Ustilago maydis. It was found that RGS proteins played distinct roles in the regulation of development and virulence. rgs1 had a minor role in virulence when deleted in a solopathogenic strain. In crosses, rgs1 was dispensable for mating and filamentation, but was required for teliospore production...
July 7, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28616972/dss1-regulates-association-of-brh2-with-rad51
#12
Qingwen Zhou, William K Holloman
Brh2, the BRCA2 ortholog in the fungus Ustilago maydis, mediates delivery of Rad51 to DNA during the course of homology-directed DNA repair. Rad51 interacts with Brh2 through the highly conserved BRC element and through a second region termed CRE located at the extreme carboxy terminus. Dss1, a small intrinsically unstructured protein that interacts with Brh2, is crucial for its activity in DNA repair, but the mechanism of this regulation is uncertain. In previous studies, we found that interaction of Brh2 with DNA was strongly modulated by association with Dss1...
July 5, 2017: Biochemistry
https://www.readbyqxmd.com/read/28611813/insights-into-host-cell-modulation-and-induction-of-new-cells-by-the-corn-smut-ustilago-maydis
#13
REVIEW
Amey Redkar, Alexandra Matei, Gunther Doehlemann
Many filamentous fungal pathogens induce drastic modulation of host cells causing abnormal infectious structures such as galls, or tumors that arise as a result of re-programming in the original developmental cell fate of a colonized host cell. Developmental consequences occur predominantly with biotrophic phytopathogens. This suggests that these host structures result as an outcome of efficient defense suppression and intimate fungal-host interaction to suit the pathogen's needs for completion of its infection cycle...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28550409/lipid-droplets-accumulation-and-other-biochemical-changes-induced-in-the-fungal-pathogen-ustilago-maydis-under-nitrogen-starvation
#14
Lucero Romero Aguilar, Juan Pablo Pardo, Mónica Montero Lomelí, Oscar Ivan Luqueño Bocardo, Marco A Juárez Oropeza, Guadalupe Guerra Sánchez
In many organisms, the growth under nitrogen-deprivation or a poor nitrogen source impacts on the carbon flow distribution and causes accumulation of neutral lipids, which are stored as lipid droplets (LDs). Efforts are in progress to find the mechanism of LDs synthesis and degradation, and new organisms capable of accumulating large amounts of lipids for biotechnological applications. In this context, when Ustilago maydis was cultured in the absence of a nitrogen source, there was a large accumulation of lipid bodies containing mainly triacylglycerols...
May 26, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28533815/efficient-itaconic-acid-production-from-glycerol-with-ustilago-vetiveriae-tz1
#15
Thiemo Zambanini, Hamed Hosseinpour Tehrani, Elena Geiser, Dorothee Merker, Sarah Schleese, Judith Krabbe, Joerg M Buescher, Guido Meurer, Nick Wierckx, Lars M Blank
BACKGROUND: The family of Ustilaginaceae is known for their capability to naturally produce industrially valuable chemicals from different carbon sources. Recently, several Ustilaginaceae were reported to produce organic acids from glycerol, which is the main side stream in biodiesel production. RESULTS: In this study, we present Ustilago vetiveriae as new production organism for itaconate synthesis from glycerol. In a screening of 126 Ustilaginaceae, this organism reached one of the highest titers for itaconate combined with a high-glycerol uptake rate...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28512683/messenger-rna-transport-in-the-opportunistic-fungal-pathogen-candida-albicans
#16
REVIEW
Anne E McBride
Candida albicans, a common commensal fungus, can cause disease in immunocompromised hosts ranging from mild mucosal infections to severe bloodstream infections with high mortality rates. The ability of C. albicans cells to switch between a budding yeast form and an elongated hyphal form is linked to pathogenicity in animal models. Hyphal-specific proteins such as cell-surface adhesins and secreted hydrolases facilitate tissue invasion and host cell damage, but the specific mechanisms leading to asymmetric protein localization in hyphae remain poorly understood...
May 16, 2017: Current Genetics
https://www.readbyqxmd.com/read/28479603/ustilago-maydis-effectors-and-their-impact-on-virulence
#17
REVIEW
Daniel Lanver, Marie Tollot, Gabriel Schweizer, Libera Lo Presti, Stefanie Reissmann, Lay-Sun Ma, Mariana Schuster, Shigeyuki Tanaka, Liang Liang, Nicole Ludwig, Regine Kahmann
Biotrophic fungal plant pathogens establish an intimate relationship with their host to support the infection process. Central to this strategy is the secretion of a range of protein effectors that enable the pathogen to evade plant immune defences and modulate host metabolism to meet its needs. In this Review, using the smut fungus Ustilago maydis as an example, we discuss new insights into the effector repertoire of smut fungi that have been gained from comparative genomics and discuss the molecular mechanisms by which U...
July 2017: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/28468279/applying-unconventional-secretion-in-ustilago-maydis-for-the-export-of-functional-nanobodies
#18
Marius Terfrüchte, Michèle Reindl, Silke Jankowski, Parveen Sarkari, Michael Feldbrügge, Kerstin Schipper
Exploiting secretory pathways for production of heterologous proteins is highly advantageous with respect to efficient downstream processing. In eukaryotic systems the vast majority of heterologous proteins for biotechnological application is exported via the canonical endoplasmic reticulum-Golgi pathway. In the endomembrane system target proteins are often glycosylated and may thus be modified with foreign glycan patterns. This can be destructive for their activity or cause immune reactions against therapeutic proteins...
April 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28464849/transcriptome-analysis-of-smut-fungi-reveals-widespread-intergenic-transcription-and-conserved-antisense-transcript-expression
#19
Michael E Donaldson, Lauren A Ostrowski, Kristi M Goulet, Barry J Saville
BACKGROUND: Biotrophic fungal plant pathogens cause billions of dollars in losses to North American crops annually. The model for functional investigation of these fungi is Ustilago maydis. Its 20.5 Mb annotated genome sequence has been an excellent resource for investigating biotrophic plant pathogenesis. Expressed-sequence tag libraries and microarray hybridizations have provided insight regarding the type of transcripts produced by U. maydis but these analyses were not comprehensive and there were insufficient data for transcriptome comparison to other smut fungi...
May 2, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28429489/transcriptional-analysis-of-the-adaptation-of-ustilago-maydis-during-growth-under-nitrogen-fixation-conditions
#20
José Alejandro Sánchez-Arreguin, Miguel Angel Hernandez-Oñate, Claudia Geraldine León-Ramirez, José Ruiz-Herrera
Regulation of genes involved in nitrogen metabolism likely plays a role in the ability of fungi to exploit and survive under different environmental situations. To learn about the mechanism of adaptation of the biotrophic fungus Ustilago maydis from a medium containing a source of fixed nitrogen, to a medium depending on the ability to fix N2 by its bacterial endosymbiont, we explored gene expression profiles using RNA-Seq analyses under these two conditions. The differentially expressed (DE) fungal genes were analyzed, identifying 90 genes that were regulated 24 h after shifting the fungus to media lacking ammonium nitrate as a nitrogen source...
April 21, 2017: Journal of Basic Microbiology
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