keyword
MENU ▼
Read by QxMD icon Read
search

Desorption

keyword
https://www.readbyqxmd.com/read/28535609/synthesis-of-%C3%AE-galactooligosaccharide-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#1
So Young Oh, So Youn Youn, Myung Soo Park, Hyoung Geun Kim, Nam In Baek, Zhi Peng Li, Geun Eog Ji
Galactooligosaccharides (GOS) are known to be selectively utilized by Bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. In this study, β-GOS are synthesized using bifidobacterial β-galactosidase(G1) purified from recombinant E. coli with a high GOS yield and with high productivity and enhanced bifidogenic activity. The purified recombinant G1 showed maximum production of β-GOS at pH 8.5 and 45°C. A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of the major peaks of the produced β-GOS showed MW of 527 and 689, indicating the synthesis of β-GOS at degrees of polymerization (DP) of 3 and DP4, respectively...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535600/new-approach-to-resolve-the-humidity-problem-in-voc-determination-in-outdoor-air-samples-using-solid-adsorbent-tubes-followed-by-td-gc-ms
#2
Alba Maceira, Laura Vallecillos, Francesc Borrull, Rosa Maria Marcé
This study describes the humidity effect in the sampling process by adsorbent tubes followed by thermal desorption and gas chromatography-mass spectrometry (TD-GC-MS) for the determination of volatile organic compounds (VOCs) in air samples and evaluates possible solutions to this problem. Two multi-sorbent bed tubes, Tenax TA/Carbograph 1TD and Carbotrap B/Carbopack X/Carboxen 569, were tested in order to evaluate their behaviour in the presence of environmental humidity. Humidity problems were demonstrated with carbon-based tubes, while Tenax-based tubes did not display any influence...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28534678/second-harmonic-correlation-spectroscopy-theory-and-principles-for-determining-surface-binding-kinetics
#3
Krystal L Sly, John C Conboy
A novel application of second harmonic correlation spectroscopy (SHCS) for the direct determination of molecular adsorption and desorption kinetics to a surface is discussed in detail. The surface-specific nature of second harmonic generation (SHG) provides an efficient means to determine the kinetic rates of adsorption and desorption of molecular species to an interface without interference from bulk diffusion, which is a significant limitation of fluorescence correlation spectroscopy (FCS). The underlying principles of SHCS for the determination of surface binding kinetics are presented, including the role of optical coherence and optical heterodyne mixing...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28534635/ultrafast-carbon-dioxide-sorption-kinetics-using-lithium-silicate-nanowires
#4
Apolo Nambo, Juan He, Tu Quang Nguyen, Veerendra Atla, Thad L Druffel, Mahendra K Sunkara
In this paper, the Li4SiO4 nanowires (NW) were shown to be promising for CO2 capture with ultrafast kinetics. Specifically, the nanowire powders exhibited an uptake of 0.35 g g-1 of CO2 at ultra-fast adsorption rate of 0.22 g g-1 min-1 at 650-700°C. Lithium silicate (Li4SiO4) nanowires and nanopowders were synthesized using a "solvo-plasma" technique involving plasma oxidation of silicon precursors mixed with lithium hydroxide. The kinetic parameter values (k) extracted from sorption and desorption kinetics obtained using NW powders are one order of magnitude higher than those previously reported for the Li4SiO4-CO2 reaction system...
May 23, 2017: Nano Letters
https://www.readbyqxmd.com/read/28534586/desorption-of-co-from-individual-ruthenium-porphyrin-molecules-on-a-copper-surface-via-an-inelastic-tunnelling-process
#5
Takuma Omiya, Paolo Poli, Heike Arnolds, Rasmita Raval, Mats Persson, Yousoo Kim
The coordination of CO to metalloporphyrins changes their electronic and magnetic properties. Here we locally desorb CO molecules from a single ruthenium tetraphenylporphyrin carbonyl (CO-RuTPP) on Cu(110) using STM. The desorption is triggered by the injection of holes into the occupied states of the adsorbate using an unusual two-carrier process.
May 23, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28534298/formulation-and-in-vitro-characterization-of-bioactive-mesoporous-silica-with-doxorubicin-and-metronidazole-intended-for-bone-treatment-and-regeneration
#6
Katarzyna Czarnobaj, Magdalena Prokopowicz, Wiesław Sawicki
The purpose of this study was to evaluate the surface mineralization activity and in vitro drug behavior potential of two forms of mesoporous silica: powder and granulate. Ordered mesoporous SiO2 powder was synthesized by surfactant-assisted sol-gel process using tetraethoxysilane as a silica precursor and hexadecyltrimethylammonium bromide as the structure-directing agent. The granulate was prepared using silica powder and ethyl cellulose as a binding agent. Metronidazole (MT)-an anti-inflammatory substance and doxorubicin hydrochloride (ChD)-an anti-cancer drug were chosen as drug models for delivery studies...
May 22, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28534157/infrared-laser-ablation-with-vacuum-capture-for-fingermark-sampling
#7
Fabrizio Donnarumma, Eden E Camp, Fan Cao, Kermit K Murray
Infrared laser ablation coupled to vacuum capture was employed to collect material from fingermarks deposited on surfaces of different porosity and roughness. Laser ablation at 3 μm was performed in reflection mode with subsequent capture of the ejecta with a filter connected to vacuum. Ablation and capture of standards from fingermarks was demonstrated on glass, plastic, aluminum, and cardboard surfaces. Using matrix assisted laser desorption ionization (MALDI), it was possible to detect caffeine after spiking with amounts as low as 1 ng...
May 22, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28533794/boron-supply-enhances-aluminum-tolerance-in-root-border-cells-of-pea-pisum-sativum-by-interacting-with-cell-wall-pectins
#8
Xue Wen Li, Jia You Liu, Jing Fang, Lin Tao, Ren Fang Shen, Ya Lin Li, Hong Dong Xiao, Ying Ming Feng, Hai Xiang Wen, Jia Hua Guan, Li Shu Wu, Yong Ming He, Heiner E Goldbach, Min Yu
Aluminum (Al) toxicity is the primary factor limiting crop growth in acidic soils. Boron (B) alleviates Al toxicity in plants, which is mainly considered to be due to the formation of Rhamnogalacturonan II-B (RGII-B) complexes, which helps to stabilize the cytoskeleton. It is unclear yet whether this is due to the increasing of net negative charges and/or further mechanisms. Kinetics of Al accumulation and adsorption were investigated using entire cells, cell wall and pectin of root border cells (RBCs) of pea (Pisum sativum), to reveal the mechanism of B in interacting with alkali-soluble and chelator-soluble pectin for an increased Al tolerance in RBCs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28533144/discovery-of-two-p-superfamily-conotoxins-lt9a-and-lt9b-with-different-modifications-on-voltage-sensitive-sodium-channels
#9
Lei Wang, Junliang Liu, Zhenghua Ren, Yu Chen, Anlong Xu
In this work, two P-superfamily conotoxins, lt9a and lt9b, were purified and characterized from the crude venom of Conus litteratus. The amino acid sequences of lt9a and lt9b were determined by the Edman degradation method. It has been suggested that both lt9a and lt9b are produced from the precursor encoded by the gene Lt9.1. During the conotoxin maturation process, different post-translational modifications occurred between lt9a and lt9b. Conotoxin lt9b was predicted to have two prolines that underwent hydroxylation and one glutamate that underwent carboxylation, while lt9a had no hydroxyproline and carboxyglutamate residue...
May 19, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28532975/emission-of-extensively-drug-resistant-acinetobacter-baumannii-from-hospital-settings-to-the-natural-environment
#10
M Seruga Music, J Hrenovic, I Goic-Barisic, B Hunjak, D Skoric, T Ivankovic
BACKGROUND: Acinetobacter baumannii is a leading emerging pathogen that is frequently recovered from patients during hospital outbreaks. The role of environmental A. baumannii reservoirs is therefore of great concern worldwide. AIM: To investigate the connection between A. baumannii causing hospital outbreaks and environmental isolates from hospital wastewater, urban sewage and river water as the final natural recipient of wastewaters. METHODS: Clinical isolates from patients with hospital-acquired pneumonia and environmental isolates from water were collected during a two-month monitoring period...
April 11, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28532810/species-level-identification-of-coagulase-negative-staphylococcus-spp-from-buffalo-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-cydb-real-time-quantitative-pcr
#11
Lucas J L Pizauro, Camila C de Almeida, Glenn A Soltes, Durda Slavic, Oswaldo D Rossi-Junior, Fernando A de Ávila, Luiz F Zafalon, Janet I MacInnes
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532539/-detection-of-serum-peptides-in-patients-with-lung-squamous-cell-carcinoma-by-maldi-tof-ms-and-analysis-of-their-correlation-with-chemotherapy-efficacy
#12
Guanhua Zhao, Bin Xu, Xiaoyan Li, Chuanhao Tang, Haifeng Qin, Hong Wang, Shaoxing Yang, Weixia Wang, Hongjun Gao, Kun He, Xiaoqing Liu
BACKGROUND: Treatment options for patients with squamous cell carcinoma of the lung (SCC) are limited in chemotherapy. However, not all patients could benefit form standard platinum regimen. Considering the dismal prognosis of patients with advanced SCC, a greater focus on selecting sensitive chemotherapy regimens remains of upmost importance to improve outcomes in this disease. In this study, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to detect pre-chemotherapy serum peptides in advanced lung squamous cell carcinoma patients accepting paclitaxel combined with platinum chemotherapy and to analyze the correlation between serum peptides and chemotherapy efficacy...
May 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28529026/the-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-bacterial-identification-in-patients-with-smear-positive-bacterial-meningitis
#13
Boaz Bishop, Yuval Geffen, Adi Plaut, Ola Kassis, Roni Bitterman, Mical Paul, Ami Neuberger
OBJECTIVES: To assess the potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in rapid identification of bacteria from smear-positive cerebrospinal fluid (CSF) in a cohort of patients with meningitis. METHODS: Single-center observational study, including adults and children with community-acquired or postneurosurgical bacterial meningitis. Meningitis was defined using established criteria. CSF samples that had a positive CSF Gram stain were directly examined by MALDI-TOF-MS...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28528500/using-poly-glutamic-acid-as-soil-washing-agent-to-remediate-heavy-metal-contaminated-soils
#14
Zong-Han Yang, Cheng-Di Dong, Chiu-Wen Chen, Yih-Terng Sheu, Chih-Ming Kao
The extraction efficiency of heavy metals from soils using three forms of gamma poly-glutamic acid (γ-PGA) as the washing agents was investigated. Controlling factors including agent concentrations, extraction time, pH, and liquid to soil ratio were evaluated to determine the optimum operational conditions. The distribution of heavy metal species in soils before and after extraction processes was analyzed. Up to 46 and 74% of heavy metal removal efficiencies were achieved with one round and a sequential extraction process using H-bonding form of γ-PGA (200 mM) with washing time of 40 min, liquid to solid ratio of 10 to 1, and pH of 6...
May 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28528283/poly-vinyl-alcohol-hydroxyapatite-monolithic-in-needle-extraction-mine-device-preparation-and-examination-of-drug-affinity
#15
Monika Pietrzyńska, Michał Czerwiński, Adam Voelkel
Polymer-ceramic materials based on poly(vinyl alcohol) (PVA) and hydroxyapatite were applied as sorption material in Monolithic In-Needle Extraction (MINE) device. The presented device provides new possibilities for the examination of bisphosphonates affinity for bone and will be a helpful tool in evaluation of potential antiresorptive drugs suitability. A ceramic part of monoliths was prepared by incorporation of hydroxyapatite (HA) into the reaction mixture or by using a soaking method (mineralization of HA on the PVA)...
May 17, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28527668/imaging-mass-spectrometry-ims-of-cortical-lipids-from-preclinical-to-severe-stages-of-alzheimer-s-disease
#16
E Gónzalez de San Román, I Manuel, M T Giralt, I Ferrer, R Rodríguez-Puertas
Alzheimer's disease (AD) is a progressive neurodegenerative disease affecting millions of patients worldwide. Previous studies have demonstrated alterations in the lipid composition of lipid extracts from plasma and brain samples of AD patients. However, there is no consensus regarding the qualitative and quantitative changes of lipids in brains from AD patients. In addition, the recent developments in imaging mass spectrometry methods are leading to a new stage in the in situ analysis of lipid species in brain tissue slices from human postmortem samples...
May 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28527143/characterization-of-arsenic-availability-in-dry-and-flooded-soils-using-sequential-extraction-and-diffusive-gradients-in-thin-films-dgt-techniques
#17
Liping Zhang, Qin Sun, Shiming Ding, Xiang Cheng, Qin Liu, Chaosheng Zhang
Much attention has been paid to the availability of arsenic (As) in soils, while few studies were carried out on the comparison between the dry and flooded soils using different methods. In this study, chemical sequential extraction and diffusive gradients in thin films (DGT) techniques were employed to study the availability of As in soils amended with a range of exogenous As followed by one-year incubation under dry and flooded conditions, respectively. The results showed that the proportions of available solid As pools, including non-specifically adsorbed As (F1) and specifically adsorbed As (F2), had consistent increases with the increase of As amendment...
May 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28526343/tannin-immobilized-cellulose-hydrogel-fabricated-by-a-homogeneous-reaction-as-a-potential-adsorbent-for-removing-cationic-organic-dye-from-aqueous-solution
#18
Ying Pei, Shan Chu, Yue Chen, Zhidong Li, Jin Zhao, Shuqi Liu, Xingjun Wu, Jie Liu, Xuejing Zheng, Keyong Tang
Tannin-immobilized cellulose (CT) hydrogels were successfully fabricated by homogeneous immobilization and crosslinking reaction via a simple method. The structures and properties of hydrogels were characterized by SEM and mechanical test. Methlyene Blue (MB) was selected as a cationic dye model, and the adsorption ability of CT hydrogel was evaluated. Tannins immobilized acted as adsorbent sites which combined MB by electrostatic attraction, resulting in the attractive adsorption ability of CT hydrogel. Adsorption kinetics could be better described by the pseudo-second-order model, and the absorption behaviors were in agreement with a Langmuir isotherm...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28525645/molecular-characterization-of-prototheca-strains-isolated-in-china-revealed-the-first-cases-of-protothecosis-associated-with-prototheca-zopfii-genotype-1
#19
Noriyuki Hirose, Zhensheng Hua, Yuichi Kato, Qiangqiang Zhang, Ruoyu Li, Kazuko Nishimura, Michiaki Masuda
In this study, six strains of Prototheca isolated in China from human patients diagnosed as protothecosis and cows with mastitis were characterized by polymerase chain reaction (PCR) and nucleotide sequencing of the ribosomal RNA gene (rDNA) and by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). The results indicated that three strains isolated from the human patients were P. zopfii genotype 1, revealing the first cases of human protothecosis associated with P. zopfii genotype 1...
May 19, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28524200/isolation-of-a-novel-bioactive-protein-from-an-edible-mushroom-pleurotus-eryngii-and-its-anti-inflammatory-potential
#20
Biao Yuan, Liyan Zhao, Kanyasiri Rakariyatham, Yanhui Han, Zili Gao, Benard Muinde Kimatu, Qiuhui Hu, Hang Xiao
Edible mushrooms are rich sources of bioactive components. In this study, a bioactive protein, PEP, was isolated from an edible mushroom, Pleurotus eryngii, through (NH4)2SO4 precipitation and ion-exchange chromatography. Proteomic analysis by matrix assisted laser desorption ionization-time of flight mass spectrometry showed that PEP was a novel protein with a molecular weight of 40 kDa. PEP exhibited anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages by inhibiting the overproduction of pro-inflammatory mediators including nitric oxide (NO), cytokine IL-1β and IL-6...
May 19, 2017: Food & Function
keyword
keyword
59674
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"