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https://www.readbyqxmd.com/read/29786428/electrochemical-impedance-spectroscopy-for-real-time-detection-of-lipid-membrane-damage-based-on-a-porous-self-assembly-monolayer-support
#1
Meng Zhang, Qingyu Zhai, Liping Wan, Li Chen, Yu Peng, Chunyan Deng, Juan Xiang, Jiawei Yan
Layer-by-layer dissolution and permeable pore formation are two typical membrane damage pathways, which induce membrane function disorder and result in serious disease, such as Alzheimer's disease, Keshan disease, Sickle-cell disease and so on. To effectively distinguish and sensitively monitor these two typical membrane damage pathways, a facile electrochemical impedance strategy was developed on a porous self-assembly monolayer (pSAM) supported bilayer lipid membrane (BLM). The pSAM was prepared by selectively electrochemical reductive desorption of the mercaptopropionic acid in a mixed mercaptopropionic acid/11-mercaptoundecanoic acid self-assembled monolayer, which created plenty of nanopores with tens of nanometers in diameter and several nanometers in height (defined as inner-pores)...
May 22, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29785005/nanopore-sequencing-technology-a-new-route-for-the-fast-detection-of-unauthorized-gmo
#2
Marie-Alice Fraiture, Assia Saltykova, Stefan Hoffman, Raf Winand, Dieter Deforce, Kevin Vanneste, Sigrid C J De Keersmaecker, Nancy H C Roosens
In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29782695/dual-ag-zno-decorated-micro-nanoporous-sulfonated-polyetheretherketone-with-superior-antibacterial-capability-and-biocompatibility-via-layer-by-layer-self-assembly-strategy
#3
Yi Deng, Lei Yang, Xiaobing Huang, Junhong Chen, Xiuyuan Shi, Weizhong Yang, Min Hong, Yuan Wang, Matthew S Dargusch, Zhi-Gang Chen
Polyetheretherketone is attractive for dental and orthopedic applications due to its mechanical attributes close to that of human bone; however, the lack of antibacterial capability and bioactivity of polyetheretherketone has substantially impeded its clinical applications. Here, a dual therapy implant coating is developed on the 3D micro-/nanoporous sulfonated polyetheretherketone via layer-by-layer self-assembly of Ag ions and Zn ions. Material characterization studies have indicated that nanoparticles consisting of elemental Ag and ZnO are uniformly incorporated on the porous sulfonated polyetheretherketone surface...
May 21, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29782029/a-technique-for-a-nano-textured-gapless-microlens-array-using-self-formation-characteristics-of-anodic-alumina
#4
Yong Min Park, Woong Ki Jang, Tae Hwan Shin, Byeong Hee Kim, Young Ho Seo
This paper presents a method to utilize the growth properties of anodic alumina possessing self-formation characteristics to fabricate a nano-textured microstructure and also introduces an application technique of the proposed method. The growth rate of anodic alumina, fabricated on aluminum surfaces, has a strong dependence on the intensity of the applied current density or electric field. The uniformity of the thickness of anodic alumina is determined by its electrical distribution characteristics. Accordingly, microscale structures can be fabricated using the growth rate deviation of anodic alumina...
May 21, 2018: Nanoscale
https://www.readbyqxmd.com/read/29781000/nanoimprint-lithography-of-nanoporous-carbon-materials-for-micro-supercapacitor-architectures
#5
Stefanie Lochmann, Julia Grothe, Kai Eckhardt, Desirée Leistenschneider, Lars Borchardt, Stefan Kaskel
Nanoimprint lithography is proposed as a highly versatile method for the production of nanostructured supercapacitors (micro-supercapacitors, MSC). Liquid sucrose- and lignin-precursor printing produces patterns with high quality and a line width down to 500 nm. The liquid-carbon-precursor NIL-printing approach enables nitrogen doping to achieve an increased supercapacitor performance for aqueous electrolytes (Li2SO4). The lines are interconverted into nanoporous carbon materials (d ≈ 1 nm) with high specific surface area (>1000 m2 g-1) to form stable structures reaching specific resistivities as low as ρ = 3...
May 21, 2018: Nanoscale
https://www.readbyqxmd.com/read/29780989/a-long-chain-protic-ionic-liquid-inside-silica-nanopores-enhanced-proton-mobility-due-to-efficient-self-assembly-and-decoupled-proton-transport
#6
Mounesha N Garaga, Vassilios Dracopoulos, Ulrike Werner-Zwanziger, Josef W Zwanziger, Manuel Maréchal, Michael Persson, Lars Nordstierna, Anna Martinelli
We report enhanced protonic and ionic dynamics in an imidazole/protic ionic liquid mixture confined within the nanopores of silica particles. The ionic liquid is 1-octylimidazolium bis(trifluoromethanesulfonyl)imide ([HC8Im][TFSI]), while the silica particles are microsized and characterized by internal well connected nanopores. We demonstrate that the addition of imidazole is crucial to promote a proton motion decoupled from molecular diffusion, which occurs due to the establishment of new N-HN hydrogen bonds and fast proton exchange events in the ionic domains, as evidenced by both infrared and 1H NMR spectroscopy...
May 21, 2018: Nanoscale
https://www.readbyqxmd.com/read/29780685/highly-reversible-water-oxidation-at-ordered-nanoporous-iridium-electrodes-based-on-an-original-atomic-layer-deposition
#7
Stefanie Schlicht, Sandra Haschke, Vladimir Mikhailovskii, Alina Manshina, Julien Bachmann
Nanoporous iridium electrodes are prepared and electrochemically investigated towards the water oxidation (oxygen evolution) reaction. The preparation is based on 'anodic' aluminum oxide templates, which provide straight, cylindrical nanopores. Their walls are coated using atomic layer deposition (ALD) with a newly developed reaction which results in a metallic iridium layer. The ALD film growth is quantified by spectroscopic ellipsometry and X-ray reflectometry. The morphology and composition of the electrodes are characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction...
May 2018: ChemElectroChem
https://www.readbyqxmd.com/read/29780541/dna-binding-miniproteins-based-on-zinc-fingers-assessment-of-the-interaction-using-nanopores
#8
Jéssica Rodríguez, Soraya Learte-Aymamí, Jesús Mosquera, Garbiñe Celaya, David Rodríguez-Larrea, M Eugenio Vázquez, José L Mascareñas
Obtaining artificial proteins that mimic the DNA binding properties of natural transcription factors could open new ways of manipulating gene expression at will. In this context it is particularly interesting to develop simple synthetic systems. Inspired by the modularity of natural transcription factors, we have designed synthetic miniproteins that combine the zinc finger module of the transcription factor GAGA and AT-hook peptide domains. These constructs are capable of binding to composite DNA sequences of up to 14 base pairs with high affinity and good selectivity...
May 7, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780294/electro-deposited-nanoporous-platinum-electrode-for-eeg-monitoring
#9
Do Youn Kim, Yunseo Ku, Joong Woo Ahn, Chiheon Kwon, Hee Chan Kim
Background: One of the key issues in electroencephalogram (EEG) monitoring is accurate signal acquisition with less cumbersome electrodes. In this study, the L2 phase electro-deposited nanoporous platinum (L2-ePt) electrode is introduced, which is a new type of electrode that utilizes a stable nanoporous platinum surface to reduce the skin-electrode impedance. Methods: L2-ePt electrodes were fabricated using electro-deposition technique. Then, the effect of the nanoporous surface on the surface roughness and the electrode impedance were observed from the L2-ePt electrodes and the flat platinum (FlatPt) electrode...
May 21, 2018: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/29777971/symmetric-supercapacitor-sulphurized-graphene-and-ionic-liquid
#10
Jasmin S Shaikh, Navajsharif S Shaikh, Rohini Kharade, Sonali A Beknalkar, Jyoti V Patil, Mahesh P Suryawanshi, Pongsakorn Kanjanaboos, Chang Kook Hong, Jin Hyeok Kim, Pramod S Patil
Symmetric supercapacitor is advanced over simple supercapacitor device due to their stability over a large potential window and high energy density. Graphene is a desired candidate for supercapacitor application since it has a high surface area, good electronic conductivity and high electro chemical stability. There is a pragmatic use of ionic liquid electrolyte for supercapacitor due to its stability over a large potential window, good ionic conductivity and eco-friendly nature. For high performance supercapacitor, the interaction between ionic liquid electrolyte and graphene are crucial for better charge transportation...
May 11, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29777568/cost-efficient-magnetic-nanoporous-carbon-derived-from-citrus-peel-for-the-selective-adsorption-of-seven-insecticides
#11
Yuantao Zhou, Shurui Cao, Cunxian Xi, Jiuyan Chen, Lei Zhang, Xianliang Li, Guomin Wang, Zhiqiong Chen
A magnetic solid-phase extraction adsorbent that consisted of citrus peel-derived nanoporous carbon and silica-coated Fe3 O4 microspheres (C/SiO2 @Fe3 O4 ) was successfully fabricated by co-precipitation. As a modifier for magnetic microspheres, citrus peel-derived nanoporous carbon was not only economical and renewable for its raw material, but exerted enormous nanosized pore structure, which could directly influence the type of adsorbed analytes. The C/SiO2 @Fe3 O4 also possessed the advantages of Fe3 O4 microspheres like superparamagnetism, that could be easily separated magnetically after adsorption...
May 18, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29776028/modeling-thermal-inkjet-and-cell-printing-process-using-modified-pseudopotential-and-thermal-lattice-boltzmann-methods
#12
Salman Sohrabi, Yaling Liu
Pseudopotential lattice Boltzmann methods (LBMs) can simulate a phase transition in high-density ratio multiphase flow systems. If coupled with thermal LBMs through equation of state, they can be used to study instantaneous phase transition phenomena with a high-temperature gradient where only one set of formulations in an LBM system can handle liquid, vapor, phase transition, and heat transport. However, at lower temperatures an unrealistic spurious current at the interface introduces instability and limits its application in real flow system...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29775859/facilitating-complex-dna-mixture-interpretation-by-sequencing-highly-polymorphic-haplotypes
#13
Lev Voskoboinik, Uzi Motro, Ariel Darvasi
Interpretation of complex DNA mixtures is an ongoing challenge in the field of forensic genetics. Commonly used STR markers are quite polymorphic, enabling very high statistical association between a single source DNA profile from a crime scene and a matching suspect. STR typing of low order mixtures with two and three contributors also commonly produces high statistical association for a contributor, using current interpretation software. However higher order mixtures, with four contributors or more, are more challenging...
May 2, 2018: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29774901/hierarchical-nanopores-formed-by-block-copolymer-lithography-on-the-surfaces-of-different-materials-pre-patterned-by-nanosphere-lithography
#14
Katharina Brassat, Daniel Kool, Julius Bürger, Jörg K N Lindner
Bottom-up patterning techniques allow for the creation of surfaces with ordered arrays of nanoscale features on large areas. Two bottom-up techniques suitable for the formation of regular nanopatterns on different length scales are nanosphere lithography (NSL) and block copolymer (BCP) lithography. In this paper it is shown that NSL and BCP lithography can be combined to easily design hierarchically nanopatterned surfaces of different materials. Nanosphere lithography is used for the pre-patterning of surfaces with antidots, i...
May 18, 2018: Nanoscale
https://www.readbyqxmd.com/read/29774611/nanoporous-metallic-networks-fabrication-optical-properties-and-applications
#15
REVIEW
Racheli Ron, Emir Haleva, Adi Salomon
Nanoporous metallic networks are a group of porous materials made of solid metals with suboptical wavelength sizes of both particles and voids. They are characterized by unique optical properties, as well as high surface area and permeability of guest materials. As such, they attract a great focus as novel materials for photonics, catalysis, sensing, and renewable energy. Their properties together with the ability for scaling-up evoke an increased interest also in the industrial field. Here, fabrication techniques of large-scale metallic networks are discussed, and their interesting optical properties as well as their applications are considered...
May 17, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29774472/uncooked-spaghetti-in-a-colander-injection-of-semiflexible-polymers-in-a-nanopore
#16
Thomas Auger, Loïc Auvray, Jean-Marc Di Meglio, Fabien Montel
We study the flow injection of semiflexible polymers in a nanopore with a diameter smaller than the persistence length of the macromolecules. The suction model from de Gennes and Brochard is modified to take into account the effect of the rigidity of the polymer in the Odijk regime. We show that in this case of extreme confinement the flow threshold vanishes slowly and that in the limit of infinitely small nanopore the free energy barrier eventually disappears.
May 17, 2018: European Physical Journal. E, Soft Matter
https://www.readbyqxmd.com/read/29773633/complete-genome-sequences-of-two-bioluminescent-vibrio-campbellii-strains-isolated-from-biofouling-communities-in-the-bay-of-bengal
#17
Sophie M Colston, Gregory A Ellis, Seongwon Kim, Hemantha W Wijesekera, Dagmar H Leary, Baochuan Lin, Benjamin C Kirkup, W Judson Hervey, Gary J Vora
Vibrio campbellii is a pathogen of aquatic animals and has been proposed as a bacterial partner in the formation of bioluminescent milky seas. We present here the complete genome sequences assembled from Illumina and Oxford Nanopore data for two bioluminescent Vibrio campbellii strains (BoB-53 and BoB-90) isolated from biofouled moorings in the Bay of Bengal.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29773624/complete-genome-sequence-of-mycobacterium-marinum-atcc-927-t-obtained-using-nanopore-and-illumina-sequencing-technologies
#18
Mitsunori Yoshida, Hanako Fukano, Yuji Miyamoto, Keigo Shibayama, Masato Suzuki, Yoshihiko Hoshino
Mycobacterium marinum is a slowly growing, broad-host-range mycobacterial species. Here, we report the complete genome sequence of a Mycobacterium marinum type strain that was isolated from tubercles of diseased fish. This sequence will provide essential information for future taxonomic and comparative genome studies of its relatives.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29768814/electric-charge-dependence-of-controlled-dye-release-behavior-in-glass-ionomer-cement-containing-nano-porous-silica-particles
#19
Yosuke Bando, Ko Nakanishi, Shigeaki Abe, Shuichi Yamagata, Yasuhiro Yoshida, Junichiro Iida
We investigated in controlled dye-release behavior of nanosized silica particles containing nanocavities (Nanoporous silica, NPS). To determine this, NPS were mixed with glass ionomer cement (GIC), which is a medical material used as a matrix. The dye-release behavior was observed using a UV-visible spectrometer. After cationic dye was charged into GIC pellet containing NPS, the pellet could gradually release cationic dye for up to two weeks. To understand the dependence of electric charge on the dye-release behavior, three types of dyes with different charge were also investigated...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29767360/transcript-profiling-using-long-read-sequencing-technologies
#20
Anthony Bayega, Yu Chang Wang, Spyros Oikonomopoulos, Haig Djambazian, Somayyeh Fahiminiya, Jiannis Ragoussis
RNA sequencing using next-generation sequencing (NGS, RNA-Seq) technologies is currently the standard approach for gene expression profiling, particularly for large-scale high-throughput studies. NGS technologies comprise short-read RNA-Seq (dominated by Illumina) and long-read RNA-Seq technologies provided by Pacific Bioscience (PacBio) and Oxford Nanopore Technologies (ONT). Although short-read sequencing technologies are the most widely used, long-read technologies are increasingly becoming the standard approach for de novo transcriptome assembly and isoform expression quantification due to the complex nature of the transcriptome which consists of variable lengths of transcripts and multiple alternatively spliced isoforms for most genes...
2018: Methods in Molecular Biology
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