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https://www.readbyqxmd.com/read/28330208/purification-and-characterization-of-%C3%AE-mannanase-from-aspergillus-terreus-and-its-applicability-in-depolymerization-of-mannans-and-saccharification-of-lignocellulosic-biomass
#1
Hemant Soni, Hemant Kumar Rawat, Brett I Pletschke, Naveen Kango
Aspergillus terreus FBCC 1369 was grown in solid-state culture under statistically optimized conditions. β-Mannanase was purified to apparent homogeneity by ultrafiltration, anion exchange and gel filtration chromatography. A purification factor of 10.3-fold was achieved, with the purified enzyme exhibiting specific activity of 53 U/mg protein. The purified β-mannanase was optimally active at pH 7.0 and 70 °C and displayed stability over a broad pH range of 4.0-8.0 and a 30 min half-life at 80 °C. The molecular weight of β-mannanase was calculated as ~49 kDa by SDS-PAGE...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327797/bacterial-immunostat-mycobacterium-tuberculosis-lipids-and-their-role-in-the-host-immune-response
#2
Adriano Queiroz, Lee W Riley
The lipid-rich cell wall of Mycobacterium tuberculosis is a dynamic structure that is involved in the regulation of the transport of nutrients, toxic host-cell effector molecules, and anti-tuberculosis drugs. It is therefore postulated to contribute to the long-term bacterial survival in an infected human host. Accumulating evidence suggests that M. tuberculosis remodels the lipid composition of the cell wall as an adaptive mechanism against host-imposed stress. Some of these lipid species (trehalose dimycolate, diacylated sulphoglycolipid, and mannan-based lipoglycans) trigger an immunopathologic response, whereas others (phthiocerol dimycocerosate, mycolic acids, sulpholipid-1, and di-and polyacyltrehalose) appear to dampen the immune responses...
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28325331/two-banana-cultivars-differ-in-composition-of-potentially-immunomodulatory-mannan-and-arabinogalactan
#3
Tânia M Shiga, Nicholas C Carpita, Franco Maria Lajolo, Beatriz Rosana Cordenunsi-Lysenko
Banana (Musa acuminata and M. acuminata x M. balbisiana) fruit cell walls are rich in mannans, homogalacturonans and xylogalacturonan, rhamnogalacturonan-I, and arabinogalactans, certain forms of which is considered to have immunomodulatory activity. The cultivars Nanicão and Thap Maeo represent two widely variants with respect to compositional differences in the forms of these polysaccharides. Nanicão has low amounts of mannan in the water-insoluble and water-soluble fraction. Both cultivars have high amounts of water-soluble arabinogalactan...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28324680/somatic-complications-of-epilepsy-surgery-over-25-years-at-a-single-center
#4
Inuka K Gooneratne, Shahidul Mannan, Jane de Tisi, Juan C Gonzalez, Andrew W McEvoy, Anna Miserocchi, Beate Diehl, Tim Wehner, Gail S Bell, Josemir W Sander, John S Duncan
INTRODUCTION: Epilepsy surgery is an effective treatment for refractory focal epilepsy. Risks of surgery need to be considered when advising individuals of treatment options. We describe the frequency and nature of physical adverse events associated with epilepsy surgery in a single center. MATERIAL AND METHODS: We reviewed the prospectively maintained records of adults who underwent epilepsy surgery at our center between 1990 and 2014 to identify peri/postsurgical adverse events...
March 1, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/28318015/plasma-levels-of-masp-1-masp-3-and-map44-in-patients-with-type-2-diabetes-influence-of-glycaemic-control-body-composition-and-polymorphisms-in-the-masp1-gene
#5
Sara Skov Krogh, Charlotte Brinck Holt, Rudi Steffensen, Kristian Løkke Funck, Pernille Høyem, Esben Laugesen, Per Løgstrup Poulsen, Steffen Thiel, Troels Krarup Hansen
Mounting evidence indicates that adverse activation of the complement system plays a role in the development of diabetic vascular complications. Plasma levels of the complement proteins mannan-binding lectin (MBL) and its associated serine proteases (MASP-1 and MASP-2) are elevated in diabetes. We hypothesised that single nucleotide polymorphisms (SNPs) in the MASP1 gene may contribute to altered plasma levels of the belonging gene products; MASP-1, MASP-3 and mannan-binding lectin-associated protein of 44 kDa (MAp44) in patients with type 2 diabetes...
March 20, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28317806/mycobacterium-lepraemurium-uses-tlr-6-and-mr-but-not-lipid-rafts-or-dc-sign-to-gain-access-into-mouse-macrophages
#6
Mayra Silva-Miranda, Patricia Arce-Paredes, Oscar Rojas-Espinosa
OBJECTIVE/BACKGROUND: Mycobacterium lepraemurium (MLM), the etiologic agent of murine leprosy, is an intracellular parasite of macrophages; the mechanism used by this bacterium to enter macrophages is not known. The fate of the MLM phagosome inside macrophages is also unknown. This study was conducted to investigate how MLM enters macrophages and to define the maturation process of MLM phagosome inside macrophages. MATERIALS AND METHODS: Peritoneal macrophages were incubated in the presence of mannan-bovine serum albumin (BSA), and antibodies to known macrophage receptors, including, anti-FcγRIII/RII (anti-CD16/32), anti-CD35 (anti-CR1), anti-TLR2, anti-TLR4, anti-TLR6, anti-CD14, and anti-dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN)...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28317006/proteomic-data-on-enzyme-secretion-and-activity-in-the-bacterium-chitinophaga-pinensis
#7
Johan Larsbrink, Tina R Tuveng, Phillip B Pope, Vincent Bulone, Vincent G H Eijsink, Harry Brumer, Lauren S McKee
The secretion of carbohydrate-degrading enzymes by a bacterium sourced from a softwood forest environment has been investigated by mass spectrometry. The findings are discussed in full in the research article "Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis" in Journal of Proteomics by Larsbrink et al. ([1], doi: 10.1016/j.jprot.2017.01.003). The bacterium was grown on three carbon sources (glucose, glucomannan, and galactomannan) which are likely to be nutrient sources or carbohydrate degradation products found in its natural habitat...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28303635/incident-microalbuminuria-and-complement-factor-mannan-binding-lectin-associated-protein-19-in-people-with-newly-diagnosed-type-1-diabetes
#8
J A Ostergaard, S Thiel, I T Hoffmann-Petersen, P Hovind, H-H Parving, L Tarnow, P Rossing, T K Hansen
BACKGROUND: Evidence links the lectin pathway of complement activation to diabetic kidney disease. Upon carbohydrate-recognition by pattern-recognition molecules, e.g., mannan-binding lectin (MBL), the MBL-associated serine protease (MASP-2) is activated and initiates the complement cascade. The MASP2 gene encodes MASP-2 and the alternative splice product MBL-associated protein 19 (MAp19). Both MAp19 and MASP-2 circulate in complex with MBL. We tested the hypothesis that MAp19 and MASP-2 concentrations predict the risk of incident microalbuminuria...
March 16, 2017: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/28303604/in-vivo-cleansing-efficacy-of-biodegradable-exfoliating-beads-assessed-by-skin-bioengineering-techniques
#9
J Kitsongsermthon, K Duangweang, J Kreepoke, A Tansirikongkol
BACKGROUND/PURPOSE: The plastic microbeads, used in many cleansers, will be banned in cosmetic and personal care products within 2017 since they are non-degradable and can disturb the living organisms in water reservoirs. Various choices of biodegradable beads are commercially available, but their efficacy has not been proven yet. This study aimed to compare the cleansing efficacy in dirt and sebum removal aspects of three types of exfoliating beads. METHODS: The gel scrubs with polyethylene (PE) beads, mannan beads or wax beads, were formulated and evaluated for their stability...
March 16, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/28280545/correlations-between-axial-stiffness-and-microstructure-of-a-species-of-bamboo
#10
Sayyad Mannan, J Paul Knox, Sumit Basu
Bamboo is a ubiquitous monocotyledonous flowering plant and is a member of the true grass family Poaceae. In many parts of the world, it is widely used as a structural material especially in scaffolding and buildings. In spite of its wide use, there is no accepted methodology for standardizing a species of bamboo for a particular structural purpose. The task of developing structure-property correlations is complicated by the fact that bamboo is a hierarchical material whose structure at the nanoscopic level is not very well explored...
January 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28278615/in-vitro-cholinesterase-inhibitory-activity-of-some-plants-used-in-iranian-traditional-medicine
#11
Mina Saeedi, Khatereh Babaie, Elahe Karimpour-Razkenari, Mahdi Vazirian, Tahmineh Akbarzadeh, Mahnaz Khanavi, Mannan Hajimahmoodi, Mohammad Reza Shams Ardekani
In this study, in vitro evaluation of cholinesterase inhibitory (ChEI) activity of various plants including betel nuts (Areca catechu L.), clove buds (Syzygium aromaticum L.), aerial parts of dodder (Cuscuta chinensis Lam.), common polypody rhizomes (Polypodium vulgare L.) and turpeth roots (Ipomoea turpethum R. Br.) which were recommended for the treatment of AD symptoms in Iranian Traditional Medicine (ITM) is reported. Among them, aqueous extract of A. catechu L. was found as the most potent anti-AChE (IC50 = 32...
March 6, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28272652/bioprotective-efficacy-of-erucin-against-7-12-dimethylbenz-%C3%AE-anthracene-induced-microstructural-changes-in-male-wistar-rats
#12
Rahul Mannan, Rohit Arora, Sakshi Bhushan, Sonam Sharma, Tejinder Bhasin, Saroj Arora
OBJECTIVE: Environmental pollutants are responsible for inducing a number of biochemical and physical changes in the various organs of an organism. The most potent pollutants is7,12-dimethylbenz(α)anthracene (DMBA) (a genotoxic carcinogen), a polyaromatic hydrocarbon. It is known to induce a number of genetic changes including the formation of DNA adducts. These genotypic changes in turn are responsible for inducing microstructural changes in the vital organs viz. kidneys, stomach and lungs of an organism...
February 4, 2017: Türk Patoloji Dergisi
https://www.readbyqxmd.com/read/28262014/high-efficiency-secretion-of-%C3%AE-mannanase-in-bacillus-subtilis-through-protein-synthesis-and-secretion-optimization
#13
Yafeng Song, Gang Fu, Huina Dong, Jianjun Li, Yuguang Du, Dawei Zhang
The manno endo-1, 4-mannosidase (β-mannanase, EC. 3.2.1.78) catalyzes the random hydrolysis of internal (1→4)-β-mannosidic linkages in the mannan polymers. A codon optimized β-mannanase gene from Bacillus licheniformis DSM13 was expressed in Bacillus subtilis. When four Sec-dependent and two Tat-dependent signal peptide sequences cloned from B. subtilis were placed upstream of the target gene, the highest activity of β-mannanase was observed using SPlipA as a signal peptide. Then, a 1.25-fold activity of β-mannanase was obtained when another copy of groESL operon was inserted into the genome of host strain...
March 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28261235/cytochemical-localization-of-polysaccharides-in-dendrobium-officinale-and-the-involvement-of-docsla6-in-the-synthesis-of-mannan-polysaccharides
#14
Chunmei He, Kunlin Wu, Jianxia Zhang, Xuncheng Liu, Songjun Zeng, Zhenming Yu, Xinghua Zhang, Jaime A Teixeira da Silva, Rufang Deng, Jianwen Tan, Jianping Luo, Jun Duan
Dendrobium officinale is a precious traditional Chinese medicinal plant because of its abundant polysaccharides found in stems. We determined the composition of water-soluble polysaccharides and starch content in D. officinale stems. The extracted water-soluble polysaccharide content was as high as 35% (w/w). Analysis of the composition of monosaccharides showed that the water-soluble polysaccharides were dominated by mannose, to a lesser extent glucose, and a small amount of galactose, in a molar ratio of 223:48:1...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28260778/granulomatosis-with-polyangiitis-wegener-granulomatosis-with-unusual-presentation
#15
M A Mannan, K Pasha, S Nahar, K Begum
We came across a 32 years old male admitted in our hospital with prolonged low-grade fever, haemoptysis, leg swelling, weight loss, purpuric rashes and malaena. He received anti-TB treatment at another hospital without any improvement. He was pale with bullous and purpuric lesions over legs and feet. He also had features of consolidation over both lung fields. His CRP was 312mg/L, Urine R/M/E showed 40-50 RBC/HPF, Chest X-ray showed features of bilateral consolidation, c-ANCA-10U/L (positive); Tracheal aspirate for AFB was found to be negative...
January 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28260772/neonatal-transport-experience-of-a-tertiary-care-hospital-of-bangladesh
#16
S K Dey, S Sharker, I Jahan, S C Moni, K H Shabuj, M J Chisti, M A Mannan, M Shahidullah
Safe transportation is mostly an unnoticed neonatal health issue in Bangladesh and no documentation is available regarding the existing practices. So this study was intended to document transport status of the referred newborn to a tertiary care hospital. This observational study included 150 out born neonates over 12 months period transported from various places to NICU, Bangabandhu Sheikh Mujib Medical University (BSMMU) from May 2015 to April 2016. A structured data collection form was used to record information categorized into pre-transport, during transport and at admission...
January 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28260755/an-anthropometric-study-of-bimalleolar-breadth-and-its-relationship-with-stature-on-bangladeshi-garo-people
#17
Z Ahmed, S Z Sultana, S Mannan, S Choudhury, S Farjan, A Kabir, N Nawshin, E Z Epsi, R S Zisa, M Ismatsara
A cross sectional descriptive study was conducted at different areas of Mymensingh (Haluaghat, Vatikashor and Kachijhuly) from July 2015 to June 2016 on 101 Bangladeshi Garo people. Among them 60 were male and 41 were female. Sample collection was done by nonrandom purposive sampling technique. Mixed ethnicity and any kind of foot deformity resulting either from congenital or physical injury were excluded to construct standard measurement for Garo ethnicity. Bimalleolar breadth was measured using slide calipers...
January 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28258200/endogenous-natural-complement-inhibitor-regulates-cardiac-development
#18
Simon A Mortensen, Louise L Skov, Kasper Kjaer-Sorensen, Annette G Hansen, Søren Hansen, Frederik Dagnæs-Hansen, Jens C Jensenius, Claus Oxvig, Steffen Thiel, Søren E Degn
Congenital heart defects are a major cause of perinatal mortality and morbidity, affecting >1% of all live births in the Western world, yet a large fraction of such defects have an unknown etiology. Recent studies demonstrated surprising dual roles for immune-related molecules and their effector mechanisms during fetal development and adult homeostasis. In this article, we describe the function of an endogenous complement inhibitor, mannan-binding lectin (MBL)-associated protein (MAp)44, in regulating the composition of a serine protease-pattern recognition receptor complex, MBL-associated serine protease (MASP)-3/collectin-L1/K1 hetero-oligomer, which impacts cardiac neural crest cell migration...
March 3, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28256031/microbiome-dynamic-modulation-through-functional-diets-based-on-pre-and-probiotics-mannan-oligosaccharides-and-saccharomyces-cerevisiae-in-juvenile-rainbow-trout-oncorhynchus-mykiss
#19
Ana Teresa Gonçalves, Cristian Gallardo-Escárate
AIMS: This study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0.6% mannan-oligosaccharides and 0.5% Saccharomyces cerevisiae respectively) or the mixture of both. METHODS AND RESULTS: A total of 57 fish whole intestinal mucosa and contents bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene, as well as the relationship between plasma biochemical health indicators and microbiome diversity...
March 3, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28248105/manufacture-and-properties-of-glucomannans-and-glucomannooligosaccharides-derived-from-konjac-and-other-sources
#20
Belén Gómez, Beatriz Míguez, Remedios Yáñez, José L Alonso
Glucomannans (GM) are polymers that can be found in natural resources, such as tubers, bulbs, roots, and both hard- and softwoods. In fact, mannan-based polysaccharides represent the largest hemicellulose fraction in softwoods. In addition to their structural functions and their role as energy reserve, they have been assessed for their healthy applications, including their role as new source of prebiotics. This paper summarizes the scientific literature regarding the manufacture and functional properties of GM and their hydrolysis products with a special focus on their prebiotic activity...
March 1, 2017: Journal of Agricultural and Food Chemistry
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