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https://www.readbyqxmd.com/read/28107361/dectin-3-is-not-required-for-protection-against-cryptococcus-neoformans-infection
#1
Althea Campuzano, Natalia Castro-Lopez, Karen L Wozniak, Chrissy M Leopold Wager, Floyd L Wormley
C-type lectin receptors (CLRs) are diverse, trans-membrane proteins that function as pattern recognition receptors (PRRs) which are necessary for orchestrating immune responses against pathogens. CLRs have been shown to play a major role in recognition and protection against fungal pathogens. Dectin-3 (also known as MCL, Clecsf8, or Clec4d) is a myeloid cell-specific CLR that recognizes mycobacterial trehalose 6,6'-dimycolate (TDM) as well as α-mannans present in the cell wall of fungal pathogens. To date, a potential role for Dectin-3 in the mediation of protective immune responses against C...
2017: PloS One
https://www.readbyqxmd.com/read/28105485/biochemical-characterization-of-thermostable-%C3%AE-1-4-mannanase-belonging-to-the-glycoside-hydrolase-family-134-from-aspergillus-oryzae
#2
Kiyota Sakai, Mai Mochizuki, Miyuki Yamada, Yuta Shinzawa, Miho Minezawa, Saran Kimoto, Shunsuke Murata, Yuhei Kaneko, Saaya Ishihara, Sadanari Jindou, Tetsuo Kobayashi, Masashi Kato, Motoyuki Shimizu
A β-1,4-mannanase, termed AoMan134A, that belongs to the GH 134 family was identified in the filamentous fungus Aspergillus oryzae. Recombinant AoMan134A was expressed in Pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. The catalytic efficiency (k cat/K m ) of AoMan134A was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactomannan from guar gum, but similar toward galactomannan from locust bean gum and glucomannan from konjac flour...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28101787/a-recombinant-highly-thermostable-%C3%AE-mannanase-retman26-from-thermophilic-bacillus-subtilis-tbs2-expressed-in-pichia-pastoris-and-its-ph-and-temperature-stability
#3
Zhangcai Luo, Jing Miao, Guoying Li, Yao Du, Xiaobin Yu
A gene encoding a highly thermostable β-mannanase from a thermophilic Bacillus subtilis (TBS2) was successfully expressed in Pichia pastoris. The maximum activity of the recombinant thermostable β-mannanase (ReTMan26) was 5435 U/mL, which was obtained by high-density, fed-batch cultivation after 168-h induction with methanol in a 50-L bioreactor. The protein yield reached 3.29 mg/mL, and the protein had a molecular weight of ~42 kDa. After fermentation, ReTMan26 was purified using a 10-kDa cut-off membrane and Sephadex G-75 column...
January 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28088277/immunoassays-of-chemically-modified-polysaccharides-glycans-in-glycoproteins-and-ribose-in-nucleic-acids
#4
Slađana Strmečki, Mojmír Trefulka, Pavlína Zatloukalová, Michal Durech, Borivoj Vojtesek, Emil Paleček
Glycosylation of proteins plays an important role in health and diseases. At present new simple and inexpensive methods of glycoprotein analysis are sought. We developed a monoclonal antibody Manost 2.1 in mice after immunization with the adduct of mannan with Os(VI)temed complex (temed is N,N,N',N'-tetramethylethylenediamine). The specificity of this antibody to different biomolecules treated with Os(VI)temed was tested using dot blot immunoassay. Manost 2.1 showed specificity toward Os(VI)temed-modified polysaccharides, glycoproteins and ribonucleotide at the 3'-end in DNA...
February 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28069559/proteomic-insights-into-mannan-degradation-and-protein-secretion-by-the-forest-floor-bacterium-chitinophaga-pinensis
#5
Johan Larsbrink, Tina R Tuveng, Phillip B Pope, Vincent Bulone, Vincent G H Eijsink, Harry Brumer, Lauren S McKee
: Together with fungi, saprophytic bacteria are central to the decomposition and recycling of biomass in forest environments. The Bacteroidetes phylum is abundant in diverse habitats, and several species have been shown to be able to deconstruct a wide variety of complex carbohydrates. The genus Chitinophaga is often enriched in hotspots of plant and microbial biomass degradation. We present a proteomic assessment of the ability of Chitinophaga pinensis to grow on and degrade mannan polysaccharides, using an agarose plate-based method of protein collection to minimise contamination with exopolysaccharides and proteins from lysed cells, and to reflect the realistic setting of growth on a solid surface...
January 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28063981/macrophage-activation-status-determines-the-internalization-of-mesoporous-silica-particles-of-different-sizes-exploring-the-role-of-different-pattern-recognition-receptors
#6
Audrey Gallud, Olesja Bondarenko, Neus Feliu, Natalia Kupferschmidt, Rambabu Atluri, Alfonso Garcia-Bennett, Bengt Fadeel
Mesoporous silica-based particles are promising candidates for biomedical applications. Here, we address the importance of macrophage activation status for internalization of AMS6 (approx. 200 nm in diameter) versus AMS8 (approx. 2 μm) mesoporous silica particles and the role of different phagocytosis receptors for particle uptake. To this end, FITC-conjugated silica particles were used. AMS8 were found to be non-cytotoxic both for M-CSF-stimulated (anti-inflammatory) and GM-CSF-stimulated (pro-inflammatory) macrophages, whereas AMS6 exhibited cytotoxicity towards M-CSF-stimulated, but not GM-CSF-stimulated macrophages; this toxicity was, however, mitigated in the presence of serum...
December 27, 2016: Biomaterials
https://www.readbyqxmd.com/read/28058278/a-%C3%AE-mannanase-with-a-lysozyme-like-fold-and-a-novel-molecular-catalytic-mechanism
#7
Yi Jin, Marija Petricevic, Alan John, Lluís Raich, Huw Jenkins, Leticia Portela De Souza, Fiona Cuskin, Harry J Gilbert, Carme Rovira, Ethan D Goddard-Borger, Spencer J Williams, Gideon J Davies
The enzymatic cleavage of β-1,4-mannans is achieved by endo-β-1,4-mannanases, enzymes involved in germination of seeds and microbial hemicellulose degradation, and which have increasing industrial and consumer product applications. β-Mannanases occur in a range of families of the CAZy sequence-based glycoside hydrolase (GH) classification scheme including families 5, 26, and 113. In this work we reveal that β-mannanases of the newly described GH family 134 differ from other mannanase families in both their mechanism and tertiary structure...
December 28, 2016: ACS Central Science
https://www.readbyqxmd.com/read/28042081/two-short-peptidoglycan-recognition-proteins-from-crassostrea-gigas-with-similar-structure-exhibited-different-pamp-binding-activity
#8
Chuanyan Yang, Lingling Wang, Zhihao Jia, Qilin Yi, Qingsong Xu, Weilin Wang, Changhao Gong, Conghui Liu, Linsheng Song
Peptidoglycan recognition protein (PGRP) is an essential molecule in innate immunity for both invertebrates and vertebrates, owing to its prominent ability in specifically recognizing bacterial peptidoglycan (PGN) and eliminating the invading bacteria. In the present study, the full length cDNA of two PGRP genes, CgPGRPS2 and CgPGRPS4, were cloned from oyster Crassostrea gigas. Their amino acid sequences both contained one signal peptide, one typical PGRP/amidase domain with conserved catalytic residues responsible for amidase activity (55H, 90Y, 164H, 172C in CgPGRPS2, and 98H, 133Y, 207H, 215C in CgPGRPS4), and specific PGN recognition (84R, 85W, 104R, 109V in CgPGRPS2, and 127G, 128W, 147R, 152V in CgPGRPS4), and they shared 55...
December 30, 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28032975/thermosensitive-zrp-pnipam-pickering-emulsifier-and-the-controlled-release-behavior
#9
Xuezhen Wang, Minxiang Zeng, Yi-Hsien Yu, Huiliang Wang, M Sam Mannan, Zhengdong Cheng
Asymmetric Janus and Gemini ZrP-PNIPAM monolayer nanoplatelets were obtained by exfoliation of two-dimensional layered ZrP disks whose surface was covalently modified with thermosensitive polymer PNIPAM. The nanoplatelets largely reduced interfacial tension (IFT) of the oil/water interface so that they were able to produce stable oil/water emulsions, and the PNIPAM grafting either on the surface or the edge endowed the nanoplatelets rapid temperature response. The ZrP-PNIPAM nanoplatelets proved to be thermosensitive Pickering emulsifiers for controlled-release applications...
December 29, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28027502/bioactive-polysaccharide-based-ph-sensitive-polymers-for-cytoplasmic-delivery-of-antigen-and-activation-of-antigen-specific-immunity
#10
Eiji Yuba, Ayaka Yamaguchi, Yuta Yoshizaki, Atsushi Harada, Kenji Kono
For establishment of cancer immunotherapy, antigen carriers are needed which have functions not only to deliver antigen into cytosol of dendritic cells (DCs), which induces antigen-specific cellular immune responses, but also to activate DCs. We previously reported cytoplasmic delivery of antigen using liposomes modified with pH-sensitive polymers such as carboxylated poly(glycidol)s or dextran. Modification using these polymers provides stable liposomes with pH-sensitive fusogenic/membrane-disruptive ability...
December 22, 2016: Biomaterials
https://www.readbyqxmd.com/read/28017156/sugar-based-biopolymers-in-nanomedicine-new-emerging-era-for-cancer-imaging-and-therapy
#11
Mehmet S Eroglu, Ebru Toksoy Oner, Esra Cansever Mutlu, Muge Sennaroglu Bostan
Since last decade, sugar based biopolymers are recognized in nanomedicine as promising materials for cancer imaging and therapy. Their durable, biocompatible and adhesive properties enable the fine tuning of their molecular weights (MW) and their miscellaneous nature makes the molecules acquire various conformations. These in turn provide effective endocytosis by cancer cell membranes that have already been programmed for internalization of different kinds of sugars. Therefore, biocompatible sugar based nanoparticles (SBNPs) are suitable for both cell-selective delivery of drugs and imaging through the human body...
December 21, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28011351/intracranial-hypertension-secondary-to-high-dose-cytosine-arabinoside-a-case-study
#12
Z A Nurgat, H Alzahrani, M Lawrence, A Mannan, M Ashour, W Rasheed, M Aljurf
We report a rare case of intracranial hypertension following high dose cytosine arabinoside (HiDAC) in a 20-year-old man, with precursor B-cell acute lymphoblastic leukemia (ALL). A five drug induction protocol for ALL was initiated; post induction consolidation was with HiDAC (3 g/m(2) IV every 12 h on days 1, 3 and 5). Post consolidation, cytogenetic remission was attained and he received an intensification and maintenance regimen for ALL, for a period of approximately 24 months. Four months following the completion of his treatment, the patient relapsed within the central nervous system (CNS)...
December 20, 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28008939/csmap34-a-teleost-map-with-dual-role-a-promoter-of-masp-assisted-complement-activation-and-a-regulator-of-immune-cell-activity
#13
Mo-Fei Li, Jun Li, Li Sun
In teleost fish, the immune functions of mannan-binding lectin (MBL) associated protein (MAP) and MBL associated serine protease (MASP) are scarcely investigated. In the present study, we examined the biological properties both MAP (CsMAP34) and MASP (CsMASP1) molecules from tongue sole (Cynoglossus semilaevis). We found that CsMAP34 and CsMASP1 expressions occurred in nine different tissues and were upregulated by bacterial challenge. CsMAP34 protein was detected in blood, especially during bacterial infection...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28000530/welcome-to-the-9th-volume-of-immunotherapy
#14
Sonia Mannan
No abstract text is available yet for this article.
January 2017: Immunotherapy
https://www.readbyqxmd.com/read/28000419/genomic-and-physiological-analyses-of-reinekea-forsetii-reveal-a-versatile-opportunistic-lifestyle-during-spring-algae-blooms
#15
Burak Avcı, Richard L Hahnke, Meghan Chafee, Tanja Fischer, Harald Gruber-Vodicka, Halina E Tegetmeyer, Jens Harder, Bernhard M Fuchs, Rudolf I Amann, Hanno Teeling
Gammaproteobacterial Reinekea spp. were detected during North Sea spring algae blooms in the years 2009-2012, with relative abundances of up to 16% in the bacterioplankton. Here we explore the ecophysiology of 'R. forsetii' strain Hel1_31_D35 that was isolated during the 2010 spring bloom using (i) its manually annotated, high-quality closed genome, (ii) re-analysis of in situ data from the 2009-2012 blooms and (iii) physiological tests. High resolution analysis of 16S rRNA gene sequences suggested that 'R...
December 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27994582/disruption-of-protein-mannosylation-affects-candida-guilliermondii-cell-wall-immune-sensing-and-virulence
#16
María J Navarro-Arias, Tatiana A Defosse, Karine Dementhon, Katalin Csonka, Erika Mellado-Mojica, Aline Dias Valério, Roberto J González-Hernández, Vincent Courdavault, Marc Clastre, Nahúm V Hernández, Luis A Pérez-García, Dhirendra K Singh, Csaba Vizler, Attila Gácser, Ricardo S Almeida, Thierry Noël, Mercedes G López, Nicolas Papon, Héctor M Mora-Montes
The fungal cell wall contains glycoproteins that interact with the host immune system. In the prominent pathogenic yeast Candida albicans, Pmr1 acts as a Golgi-resident ion pump that provides cofactors to mannosyltransferases, regulating the synthesis of mannans attached to glycoproteins. To gain insight into a putative conservation of such a crucial process within opportunistic yeasts, we were particularly interested in studying the role of the PMR1 homolog in a low-virulent species that rarely causes candidiasis, Candida guilliermondii...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27994172/microsatellite-markers-suggest-high-genetic-diversity-in-an-urban-population-of-cooper-s-hawks-accipiter-cooperii
#17
Francisco Morinha, Pedro Silveira Ramos, Sónia Gomes, Robert William Mannan, Henrique Guedes-Pinto, Estela Bastos
No abstract text is available yet for this article.
November 11, 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27993086/cancer-vaccine-clinical-trials
#18
Sonia Mannan
No abstract text is available yet for this article.
November 2016: Immunotherapy
https://www.readbyqxmd.com/read/27992199/contribution-of-shape-and-charge-to-the-inhibition-of-a-family-gh99-endo-%C3%AE-1-2-mannanase
#19
Marija Petricevic, Lukasz F Sobala, Pearl Z Fernandes, Lluís Raich, Andrew J Thompson, Ganeko Bernardo-Seisdedos, Oscar Millet, Sha Zhu, Matthieu Sollogoub, Jesús Jiménez-Barbero, Carme Rovira, Gideon J Davies, Spencer J Williams
Inhibitor design incorporating features of the reaction coordinate and transition-state structure has emerged as a powerful approach for the development of enzyme inhibitors. Such inhibitors find use as mechanistic probes, chemical biology tools, and therapeutics. Endo-α-1,2-mannosidases and endo-α-1,2-mannanases, members of glycoside hydrolase family 99 (GH99), are interesting targets for inhibitor development as they play key roles in N-glycan maturation and microbiotal yeast mannan degradation, respectively...
January 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27989252/dietary-live-yeast-and-mannan-oligosaccharide-supplementation-attenuate-intestinal-inflammation-and-barrier-dysfunction-induced-by-escherichia-coli-in-broilers
#20
Weiwei Wang, Zhui Li, Qiqi Han, Yuming Guo, Bo Zhang, Romain D'inca
The effects of live yeast (LY) and mannan-oligosaccharide (MOS) supplementation on intestinal disruption induced by Escherichia coli in broilers were investigated. The experimental design was a 3×2 factorial arrangement with three dietary treatments (control, 0·5 g/kg LY (Saccharomyces cerevisiae, 1·0×1010 colony-forming units/g), 0·5 g/kg MOS) and two immune treatments (with or without E. coli challenge from 7 to 11 d of age). Samples were collected at 14 d of age. The results showed that E. coli challenge impaired (P<0·05) growth performance during the grower period (1-21 d) and the overall period (1-35 d) of broilers, increased (P<0·05) serum endotoxin and diamine oxidase levels coupled with ileal myeloperoxidase and lysozyme activities, whereas reduced (P<0·05) maltase activity, and compromised the morphological structure of the ileum...
December 2016: British Journal of Nutrition
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