keyword
MENU ▼
Read by QxMD icon Read
search

fate protocol

keyword
https://www.readbyqxmd.com/read/29335546/transcription-factors-orchestrate-dynamic-interplay-between-genome-topology-and-gene-regulation-during-cell-reprogramming
#1
Ralph Stadhouders, Enrique Vidal, François Serra, Bruno Di Stefano, François Le Dily, Javier Quilez, Antonio Gomez, Samuel Collombet, Clara Berenguer, Yasmina Cuartero, Jochen Hecht, Guillaume J Filion, Miguel Beato, Marc A Marti-Renom, Thomas Graf
Chromosomal architecture is known to influence gene expression, yet its role in controlling cell fate remains poorly understood. Reprogramming of somatic cells into pluripotent stem cells (PSCs) by the transcription factors (TFs) OCT4, SOX2, KLF4 and MYC offers an opportunity to address this question but is severely limited by the low proportion of responding cells. We have recently developed a highly efficient reprogramming protocol that synchronously converts somatic into pluripotent stem cells. Here, we used this system to integrate time-resolved changes in genome topology with gene expression, TF binding and chromatin-state dynamics...
January 15, 2018: Nature Genetics
https://www.readbyqxmd.com/read/29329313/development-of-a-field-testing-protocol-for-identifying-deepwater-horizon-oil-spill-residues-trapped-near-gulf-of-mexico-beaches
#2
Yuling Han, T Prabhakar Clement
The Deepwater Horizon (DWH) accident, one of the largest oil spills in U.S. history, contaminated several beaches located along the Gulf of Mexico (GOM) shoreline. The residues from the spill still continue to be deposited on some of these beaches. Methods to track and monitor the fate of these residues require approaches that can differentiate the DWH residues from other types of petroleum residues. This is because, historically, the crude oil released from sources such as natural seeps and anthropogenic discharges have also deposited other types of petroleum residues on GOM beaches...
2018: PloS One
https://www.readbyqxmd.com/read/29321278/spemann-mangold-grafts
#3
Hélène Cousin
In 1924, Hans Spemann and Hilde Mangold (née Pröscholdt) published their famous work describing the transplantation of dorsal blastopore lip of one newt gastrula embryo onto the ventral side of a host embryo at the same stage. They performed these grafts using two newt species with different pigmentation (Triturus taeniatus and Triturus cristatus) to follow the fate of the grafted tissue. These experiments resulted in the development of conjoined twins attached through their belly. Because of the difference in embryo pigmentation between the two Triturus species, they determined that the bulk of the secondary embryo arose from the host embryo while the grafted tissue per se gave increase to the notochord and a few somitic cells...
January 10, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29315416/human-induced-pluripotent-stem-cells-and-male-infertility-an-overview-of-current-progress-and-perspectives
#4
Fang Fang, Zili Li, Qian Zhao, Honggang Li, Chengliang Xiong
Recently, significant progress has been made in ART for the treatment of male infertility. However, current ART has failed to help infertile patients with non-obstructive azoospermia, unless donor sperm is used. In fact, most couples wish to have their own genetically related child. Human induced pluripotent stem cells (hiPSCs) can be generated from patients' somatic cells and in vitro derivation of functional germ cells from patient-specific iPSCs may provide new therapeutic strategies for infertile couples...
January 5, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29286427/live-imaging-followed-by-single-cell-tracking-to-monitor-cell-biology-and-the-lineage-progression-of-multiple-neural-populations
#5
Rosa Gómez-Villafuertes, Lucía Paniagua-Herranz, Sergio Gascon, David de Agustín-Durán, María de la O Ferreras, Juan Carlos Gil-Redondo, María José Queipo, Aida Menendez-Mendez, Ráquel Pérez-Sen, Esmerilda G Delicado, Javier Gualix, Marcos R Costa, Timm Schroeder, María Teresa Miras-Portugal, Felipe Ortega
Understanding the mechanisms that control critical biological events of neural cell populations, such as proliferation, differentiation, or cell fate decisions, will be crucial to design therapeutic strategies for many diseases affecting the nervous system. Current methods to track cell populations rely on their final outcomes in still images and they generally fail to provide sufficient temporal resolution to identify behavioral features in single cells. Moreover, variations in cell death, behavioral heterogeneity within a cell population, dilution, spreading, or the low efficiency of the markers used to analyze cells are all important handicaps that will lead to incomplete or incorrect read-outs of the results...
December 16, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29286400/experimental-column-setup-for-studying-anaerobic-biogeochemical-interactions-between-iron-oxy-hydroxides-trace-elements-and-bacteria
#6
Jennifer Hellal, André Burnol, Aude Locatelli, Fabienne Battaglia-Brunet
Fate and speciation of trace elements (TEs), such as arsenic (As) and mercury (Hg), in aquifers are closely related to physio-chemical conditions, such as redox potential (Eh) and pH, but also to microbial activities that can play a direct or indirect role on speciation and/or mobility. Indeed, some bacteria can directly oxidize As(III) to As(V) or reduce As(V) to As(III). Likewise, bacteria are strongly involved in Hg cycling, either through its methylation, forming the neurotoxin monomethyl mercury, or through its reduction to elemental Hg°...
December 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29272992/from-otic-induction-to-hair-cell-production-pax2-sup-egfp-sup-cell-line-illuminates-key-stages-of-development-in-mouse-inner-ear-organoid-model
#7
Stacy A Schaefer, Atsuko Y Higashi, Benjamin Loomis, Thomas Schrepfer, Guoqiang Wan, Gabriel Corfas, Gregory R Dressler, Robert Keith Duncan
Producing hair cells of the inner ear is the major goal of ongoing research that combines advances in developmental and stem cell biology. The recent advent of an inner ear organoid protocol-resulting in 3-dimensional stem cell-derived tissues resembling vestibular sensory epithelia-has sparked interest in applications such as regeneration, drug discovery, and disease modeling. In this study, we adapted this protocol for a novel mouse embryonic stem cell line with a fluorescent reporter for Pax2 expression...
December 22, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29230151/integrating-a-19f-mri-tracer-agent-into-the-clinical-scale-manufacturing-of-a-t-cell-immunotherapy
#8
Charles F O'Hanlon, Tamara Fedczyna, Shannon Eaker, William D Shingleton, Brooke M Helfer
Leukocyte immunotherapies have made great progress in the treatment of cancer. Recent reports on the treatment of B-cell malignancies using Chimeric Antigen Receptor and affinity enhanced T-Cell Receptor therapies have demonstrated encouraging clinical results. As investigators begin to explore the treatment of solid tumors with these cells, the hurdle of evaluating T-cell homing to and persistence at the site of disease remain. Significant challenges regarding the GMP manufacture and administration of a therapeutic dose of millions to billions of transduced T-cells remain...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29224144/low-input-whole-genome-bisulfite-sequencing-using-a-post-bisulfite-adapter-tagging-approach
#9
Julian R Peat, Sébastien A Smallwood
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA methylation landscape, but the application of these techniques to limited material remains challenging. Here, we present an optimized protocol to perform whole-genome bisulfite sequencing on low inputs (100-5000 somatic cells) using a post-bisulfite adapter tagging approach...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29217753/wnt-inhibition-promotes-vascular-specification-of-embryonic-cardiac-progenitors
#10
David Reichman, Laura Park, Limor Man, David Redmond, Kenny Chao, Richard P Harvey, Makoto M Taketo, Zev Rosenwaks, Daylon James
Several studies have demonstrated a multiphasic role for Wnt signaling during embryonic cardiogenesis (Naito et al., 2006; Qyang et al., 2007) and developed protocols that enrich for cardiac derivatives during in vitro differentiation of human pluripotent stem cells (hPSC) (Elliott et al., 2011; Iyer et al., 2016; Lian et al., 2012; Paige et al., 2010; Willems et al., 2011; Witty et al., 2014), however, few studies have investigated the role of Wnt signaling in specification of cardiac progenitor cells (CPC) toward downstream fates...
December 7, 2017: Development
https://www.readbyqxmd.com/read/29211687/cellular-reprogramming-dynamics-follow-a-simple-1d-reaction-coordinate
#11
Sai Teja Pusuluri, Alex H Lang, Pankaj Mehta, Horacio E Castillo
Cellular reprogramming, the conversion of one cell type to another, induces global changes in gene expression involving thousands of genes, and understanding how cells globally alter their gene expression profile during reprogramming is an ongoing problem. Here we reanalyze time-course data on cellular reprogramming from differentiated cell types to induced pluripotent stem cells (iPSCs) and show that gene expression dynamics during reprogramming follow a simple 1D reaction coordinate. This reaction coordinate is independent of both the time it takes to reach the iPSC state as well as the details of the experimental protocol used...
December 6, 2017: Physical Biology
https://www.readbyqxmd.com/read/29210198/concise-review-quantitative-detection-and-modeling-the-in-vivo-kinetics-of-therapeutic-mesenchymal-stem-stromal-cells
#12
REVIEW
Anastasia Brooks, Kathryn Futrega, Xiaowen Liang, Xiaoling Hu, Xin Liu, Darrell H G Crawford, Michael R Doran, Michael S Roberts, Haolu Wang
Mesenchymal stem/stromal cells (MSCs) present a promising tool in cell-based therapy for treatment of various diseases. Currently, optimization of treatment protocols in clinical studies is complicated by the variations in cell dosing, diverse methods used to deliver MSCs, and the variety of methods used for tracking MSCs in vivo. Most studies use a dose escalation approach, and attempt to correlate efficacy with total cell dose. Optimization could be accelerated through specific understanding of MSC distribution in vivo, long-term viability, as well as their biological fate...
December 6, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29209975/commercial-silicate-phosphate-sequestration-and-desorption-leads-to-a-gradual-decline-of-aquatic-systems
#13
Karl B W Svatos
Laboratory desorption behaviour, function and elemental composition of commercially marketed silicate minerals used to sequester phosphorus pollution as well as Zeolite, Smectite, and Kaolinite were determined to see whether their use by environmental scientists and water managers in eutrophic waterways has the potential to contribute to longer-term environmental impacts. As expected, lower phosphorus concentrations were observed, following treatment. However, data relating to desorption, environmental fate and bioavailability of phospho-silicate complexes (especially those containing rare earth elements) appear to be underrepresented in product testing and trial publications...
December 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29198814/a-high-content-imaging-flow-cytometry-approach-to-study-mitochondria-in-t-cells-mitotracker-green-fm-dye-concentration-optimization
#14
Namrata Gautam, Shvetha Sankaran, John A Yason, Kevin S W Tan, Nicholas R J Gascoigne
Mitochondria, the powerhouse of the cell, are known to remodel their membrane structures through the process of fusion or fission. Studies have indicated that T cells adopt different energy metabolic phenotypes, namely oxidative phosphorylation and glycolysis depending on whether they are naïve, effector and memory T cells. It has recently been shown that changes in mitochondrial morphology dictate T cell fate via regulation of their metabolism. Our keen interest in T cell function and metabolism led us to explore and establish a method to study mitochondria in live T cells through a novel high content approach called Imaging Flow Cytometry (IFC)...
November 30, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29195838/a-phenomenological-analysis-of-eco-evolutionary-coupling-under-dilution
#15
Vitor H Sanches, Dhyan V H Kuraoka, Pedro R Almeida, Carla Goldman
Evolutionary dynamics experienced by mixed microbial populations of cooperators and cheaters has been examined in experiments in the literature using a protocol of periodic dilution to investigate the properties of resilience and adaptability to environmental changes. Data depicted on an appropriate phase diagram indicate, among other features, a stable equilibrium point at which cooperators and cheaters coexist (Sanchez A, Gore J. Feedback between population and evolutionary dynamics determines the fate of social microbial populations...
November 28, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29191922/adventitial-sca1-stem-cell-antigen-1-cells-transduced-with-etv2-ets-variant-2-are-committed-to-the-endothelial-fate-and-improve-vascular-remodeling-after-injury
#16
Alexandra Le Bras, Baoqi Yu, Shirin Issa Bhaloo, Xuechong Hong, Zhongyi Zhang, Yanhua Hu, Qingbo Xu
OBJECTIVE: Vascular adventitial Sca1+ (stem cell antigen-1) progenitor cells preferentially differentiate into smooth muscle cells, which contribute to vascular remodeling and neointima formation in vessel grafts. Therefore, directing the differentiation of Sca1+ cells toward the endothelial lineage could represent a new therapeutic strategy against vascular disease. APPROACH AND RESULTS: We thus developed a fast, reproducible protocol based on the single-gene transfer of ETV2 (ETS variant 2) to differentiate Sca1+ cells toward the endothelial fate and studied the effect of cell conversion on vascular hyperplasia in a model of endothelial injury...
November 30, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29175012/osteogenic-differentiation-of-equine-adipose-tissue-derived-mesenchymal-stem-cells-using-cacl2
#17
Mohamed I Elashry, Nadine Baulig, Manuela Heimann, Caroline Bernhardt, Sabine Wenisch, Stefan Arnhold
Adipose tissue derived mesenchymal stem cells (ASCs) may be used to cure bone defects after osteogenic differentiation. In this study we tried to optimize osteogenic differentiation for equine ASCs using various concentrations of CaCl2 in comparison to the standard osteogenic protocol. ASCs were isolated from subcutaneous adipose tissue from mixed breed horses. The osteogenic induction protocols were (1) the standard osteogenic medium (OM) composed of dexamethasone, ascorbic acid and β-glycerol phosphate; (2) CaCl2 based protocol composed of 3, 5 and 7...
November 21, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29167563/specification-of-murine-ground-state-pluripotent-stem-cells-to-regional-neuronal-populations
#18
Walaa F Alsanie, Jonathan C Niclis, Cameron P Hunt, Isabelle R De Luzy, Vanessa Penna, Christopher R Bye, Colin W Pouton, John Haynes, Jaber Firas, Lachlan H Thompson, Clare L Parish
Pluripotent stem cells (PSCs) are a valuable tool for interrogating development, disease modelling, drug discovery and transplantation. Despite the burgeoned capability to fate restrict human PSCs to specific neural lineages, comparative protocols for mouse PSCs have not similarly advanced. Mouse protocols fail to recapitulate neural development, consequently yielding highly heterogeneous populations, yet mouse PSCs remain a valuable scientific tool as differentiation is rapid, cost effective and an extensive repertoire of transgenic lines provides an invaluable resource for understanding biology...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29155748/using-in-vitro-live-cell-imaging-to-explore-chemotherapeutics-delivered-by-lipid-based-nanoparticles
#19
Ann L B Seynhaeve, Timo L M Ten Hagen
Conventional imaging techniques can provide detailed information about cellular processes. However, this information is based on static images in an otherwise dynamic system, and successive phases are easily overlooked or misinterpreted. Live-cell imaging and time-lapse microscopy, in which living cells can be followed for hours or even days in a more or less continuous fashion, are therefore very informative. The protocol described here allows for the investigation of the fate of chemotherapeutic nanoparticles after the delivery of doxorubicin (dox) in living cells...
November 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155562/quantification-of-nanomaterial-nanomedicine-trafficking-in-vivo
#20
Liming Wang, Liang Yan, Jing Liu, Chunying Chen, Yuliang Zhao
Absorption, distribution, metabolism and excretion (ADME) of nanomaterials (NMs) or nanomedicines (NMs), are fundamental biological processes that dominate their nanomedical functions or nanotoxicities. Mostly, NMs exist in biological systems as a form of nanoparticles (NPs). However, to determine the composition, spatial distribution, and states of existence of NMs/NPs in vivo, upon entry into the body, as well as to monitor the dynamic changes of these information over time remain a challenge for the current analytical methodology due to their limited efficiently in characterizing interactions between these complex(nano)-complex(bio) systems...
November 20, 2017: Analytical Chemistry
keyword
keyword
59623
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"