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https://www.readbyqxmd.com/read/28412566/removal-mechanisms-of-benzotriazoles-in-duckweed-lemna-minor-wastewater-treatment-systems
#1
Georgia Gatidou, Maria Oursouzidou, Aimilia Stefanatou, Athanasios S Stasinakis
The fate of five benzotriazoles (1H-benzotriazole, BTR; 4-methyl-1H-benzotriazole, 4TTR; 5-methyl-1H-benzotriazole, 5TTR; xylytriazole, XTR and 5-chlorobenzotriazole, CBTR) was studied in batch and continuous-flow Lemna minor systems and the role of different mechanisms on their removal was evaluated. Single and joint toxicity experiments were initially conducted using the Organization for Economic Co-operation and Development (OECD) protocol 221 and no inhibition on specific growth rate of Lemna minor was observed for concentrations up to 200μgL(-1)...
April 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28404796/tuning-cancer-fate-the-unremitting-role-of-host-immunity
#2
REVIEW
B Calì, B Molon, A Viola
Host immunity plays a central and complex role in dictating tumour progression. Solid tumours are commonly infiltrated by a large number of immune cells that dynamically interact with the surrounding microenvironment. At first, innate and adaptive immune cells successfully cooperate to eradicate microcolonies of transformed cells. Concomitantly, surviving tumour clones start to proliferate and harness immune responses by specifically hijacking anti-tumour effector mechanisms and fostering the accumulation of immunosuppressive immune cell subsets at the tumour site...
April 2017: Open Biology
https://www.readbyqxmd.com/read/28374539/comparative-assessment-of-the-thermal-tolerance-of-spotted-stemborer-chilo-partellus-lepidoptera-crambidae-and-its-larval-parasitoid-cotesia-sesamiae-hymenoptera-braconidae
#3
Reyard Mutamiswa, Frank Chidawanyika, Casper Nyamukondiwa
Under stressful thermal environments, insects adjust their behaviour and physiology to maintain key life-history activities and improve survival. For interacting species, mutual or antagonistic, thermal stress may affect the partcipants in differing ways, which may then affect the outcome of the ecological relationship. In agroecosystems, this may be the fate of relationships between insect pests and their antagonistic parasitoids under acute and chronic thermal variability. Against this background, we investigated the thermal tolerance of different developmental stages of Chilo partellus Swinhoe (Lepidoptera: Crambidae) and its larval parasitoid, Cotesia sesamiae Cameron (Hymenoptera: Braconidae) using both dynamic and static protocols...
April 4, 2017: Insect Science
https://www.readbyqxmd.com/read/28368778/monitoring-the-fate-and-behavior-of-tio2-nanoparticles-simulated-in-a-wwtp-with-industrial-dye-stuff-effluent-according-to-oecd-303a
#4
Lwazi C Mahlalela, Jane C Ngila, Langelihle N Dlamini
The use of nanoparticles (NPs) in several consumer products has led to them finding their way into wastewater treatment plants (WWTPs). Some of these NPs have photocatalytic properties, thus providing a possible solution to textile industries to photodegrade dyes from their wastewater. Thus, the interaction of NPs with industrial dye effluents is inevitable. The Organization for Economic Co-operation and development (OECD) guideline for testing of chemical 303A was employed to study the fate and behaviour of TiO2 NPs in industrial dye-stuff effluent...
April 3, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28362422/in-vivo-imaging-of-transgenic-gene-expression-in-individual-retinal-progenitors-in-chimeric-zebrafish-embryos-to-study-cell-nonautonomous-influences
#5
Stefanie Dudczig, Peter D Currie, Lucia Poggi, Patricia R Jusuf
The genetic and technical strengths have made the zebrafish vertebrate a key model organism in which the consequences of gene manipulations can be traced in vivo throughout the rapid developmental period. Multiple processes can be studied including cell proliferation, gene expression, cell migration and morphogenesis. Importantly, the generation of chimeras through transplantations can be easily performed, allowing mosaic labeling and tracking of individual cells under the influence of the host environment...
March 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28349454/mapping-m-6-a-at-individual-nucleotide-resolution-using-crosslinking-and-immunoprecipitation-miclip
#6
Anya V Grozhik, Bastian Linder, Anthony O Olarerin-George, Samie R Jaffrey
N (6) -methyladenosine (m6A) is the most abundant modified base in eukaryotic mRNA and has been linked to diverse effects on mRNA fate. Current m6A mapping approaches localize m6A residues to 100-200 nt-long regions of transcripts. The precise position of m6A in mRNAs cannot be identified on a transcriptome-wide level because there are no chemical methods to distinguish between m6A and adenosine. Here, we describe a method for using anti-m6A antibodies to induce specific mutational signatures at m6A residues after ultraviolet light-induced antibody-RNA crosslinking and reverse transcription...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28340415/uncertainty-analysis-for-effluent-trading-planning-using-a-bayesian-estimation-based-simulation-optimization-modeling-approach
#7
J L Zhang, Y P Li, G H Huang, B W Baetz, J Liu
In this study, a Bayesian estimation-based simulation-optimization modeling approach (BESMA) is developed for identifying effluent trading strategies. BESMA incorporates nutrient fate modeling with soil and water assessment tool (SWAT), Bayesian estimation, and probabilistic-possibilistic interval programming with fuzzy random coefficients (PPI-FRC) within a general framework. Based on the water quality protocols provided by SWAT, posterior distributions of parameters can be analyzed through Bayesian estimation; stochastic characteristic of nutrient loading can be investigated which provides the inputs for the decision making...
March 6, 2017: Water Research
https://www.readbyqxmd.com/read/28329331/natural-history-of-moderately-dilated-tubular-ascending-aorta-implications-for-determining-the-optimal-imaging-interval
#8
Kay-Hyun Park, Suryeun Chung, Dong Jung Kim, Jun Sung Kim, Cheong Lim
OBJECTIVES: For a moderately dilated ascending aorta (diameter 35-54 mm), current guidelines recommend continuous annual or semi-annual examinations with computed tomography or magnetic resonance imaging. However, few data have shown the yield and benefit of such a protocol. This study aimed to investigate the fate of a moderately dilated ascending aorta and thereby determine the adequate imaging interval. METHODS: In our institutional database, we identified adult patients having an ascending aortic diameter ≥40 mm in contrast-enhanced computed tomography and follow-up imaging(s) after ≥1 year...
May 1, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28325665/volatile-methylsiloxanes-through-wastewater-treatment-plants-a-review-of-levels-and-implications
#9
REVIEW
Daniela Capela, Nuno Ratola, Arminda Alves, Vera Homem
The use of siloxanes has been increasing due to physicochemical properties that are appropriate to be included in the formulations of a large variety of consumer products. This implies a considerable release of these chemicals into the environment, which caught the attention of the scientific community in view of some reports of potential hazardous effects. However, the studies related to the presence and partition of volatile methylsiloxanes (VMSs) in wastewater treatment plants (WWTPs) are still scarce. The aim of this review is to provide a comprehensive assessment of the current state of the knowledge concerning the presence of VMSs in WWTPs and also a discussion of the efficiency of the removal technologies available...
March 15, 2017: Environment International
https://www.readbyqxmd.com/read/28284131/decision-support-for-environmental-management-of-industrial-non-hazardous-secondary-materials-new-analytical-methods-combined-with-simulation-and-optimization-modeling
#10
Keith W Little, Nadeesha H Koralegedara, Coleen M Northeim, Souhail R Al-Abed
Non-hazardous solid materials from industrial processes, once regarded as waste and disposed in landfills, offer numerous environmental and economic advantages when put to beneficial uses (BUs). Proper management of these industrial non-hazardous secondary materials (INSM) requires estimates of their probable environmental impacts among disposal as well as BU options. The U.S. Environmental Protection Agency (EPA) has recently approved new analytical methods (EPA Methods 1313-1316) to assess leachability of constituents of potential concern in these materials...
March 8, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28229420/noninvasive-assessment-of-cell-fate-and-biology-in-transplanted-mesenchymal-stem-cells
#11
Federico Franchi, Martin Rodriguez-Porcel
Recently, molecular imaging has become a conditio sine qua non for cell-based regenerative medicine. Developments in molecular imaging techniques, such as reporter gene technology, have increasingly enabled the noninvasive assessment of the fate and biology of cells after cardiovascular applications. In this context, bioluminescence imaging is the most commonly used imaging modality in small animal models of preclinical studies. Here, we present a detailed protocol of a reporter gene imaging approach for monitoring the viability and biology of Mesenchymal Stem Cells transplanted in a mouse model of myocardial ischemia reperfusion injury...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28207000/spatial-transcriptomic-analysis-of-cryosectioned-tissue-samples-with-geo-seq
#12
Jun Chen, Shengbao Suo, Patrick Pl Tam, Jing-Dong J Han, Guangdun Peng, Naihe Jing
Conventional gene expression studies analyze multiple cells simultaneously or single cells, for which the exact in vivo or in situ position is unknown. Although cellular heterogeneity can be discerned when analyzing single cells, any spatially defined attributes that underpin the heterogeneous nature of the cells cannot be identified. Here, we describe how to use Geo-seq, a method that combines laser capture microdissection (LCM) and single-cell RNA-seq technology. The combination of these two methods enables the elucidation of cellular heterogeneity and spatial variance simultaneously...
March 2017: Nature Protocols
https://www.readbyqxmd.com/read/28191772/human-induced-pluripotent-cell-derived-sensory-neurons-for-fate-commitment-of-bone-marrow-derived-schwann-cells-implications-for-remyelination-therapy
#13
Sa Cai, Lei Han, Qiang Ao, Ying-Shing Chan, Daisy Kwok-Yan Shum
Strategies that exploit induced pluripotent stem cells (iPSCs) to derive neurons have relied on cocktails of cytokines and growth factors to bias cell-signaling events in the course of fate choice. These are often costly and inefficient, involving multiple steps. In this study, we took an alternative approach and selected 5 small-molecule inhibitors of key signaling pathways in an 8-day program to induce differentiation of human iPSCs into sensory neurons, reaching ≥80% yield in terms of marker proteins. Continuing culture in maintenance medium resulted in neuronal networks immunopositive for synaptic vesicle markers and vesicular glutamate transporters suggestive of excitatory neurotransmission...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191760/directing-differentiation-of-pluripotent-stem-cells-toward-retinal-pigment-epithelium-lineage
#14
Parul Choudhary, Heather Booth, Alex Gutteridge, Beata Surmacz, Irene Louca, Juliette Steer, Julie Kerby, Paul John Whiting
Development of efficient and reproducible conditions for directed differentiation of pluripotent stem cells into specific cell types is important not only to understand early human development but also to enable more practical applications, such as in vitro disease modeling, drug discovery, and cell therapies. The differentiation of stem cells to retinal pigment epithelium (RPE) in particular holds promise as a source of cells for therapeutic replacement in age-related macular degeneration. Here we show development of an efficient method for deriving homogeneous RPE populations in a period of 45 days using an adherent, monolayer system and defined xeno-free media and matrices...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191758/preclinical-analysis-of-fetal-human-mesencephalic-neural-progenitor-cell-lines-characterization-and-safety-in-vitro-and-in-vivo
#15
Jisook Moon, Sigrid C Schwarz, Hyun-Seob Lee, Jun Mo Kang, Young-Eun Lee, Bona Kim, Mi-Young Sung, Günter Höglinger, Florian Wegner, Jin Su Kim, Hyung-Min Chung, Sung Woon Chang, Kwang Yul Cha, Kwang-Soo Kim, Johannes Schwarz
We have developed a good manufacturing practice for long-term cultivation of fetal human midbrain-derived neural progenitor cells. The generation of human dopaminergic neurons may serve as a tool of either restorative cell therapies or cellular models, particularly as a reference for phenotyping region-specific human neural stem cell lines such as human embryonic stem cells and human inducible pluripotent stem cells. We cultivated 3 different midbrain neural progenitor lines at 10, 12, and 14 weeks of gestation for more than a year and characterized them in great detail, as well as in comparison with Lund mesencephalic cells...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28167799/cell-population-structure-prior-to-bifurcation-predicts-efficiency-of-directed-differentiation-in-human-induced-pluripotent-cells
#16
Rhishikesh Bargaje, Kalliopi Trachana, Martin N Shelton, Christopher S McGinnis, Joseph X Zhou, Cora Chadick, Savannah Cook, Christopher Cavanaugh, Sui Huang, Leroy Hood
Steering the differentiation of induced pluripotent stem cells (iPSCs) toward specific cell types is crucial for patient-specific disease modeling and drug testing. This effort requires the capacity to predict and control when and how multipotent progenitor cells commit to the desired cell fate. Cell fate commitment represents a critical state transition or "tipping point" at which complex systems undergo a sudden qualitative shift. To characterize such transitions during iPSC to cardiomyocyte differentiation, we analyzed the gene expression patterns of 96 developmental genes at single-cell resolution...
February 28, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28165731/novel-experimental-modeling-approach-for-characterizing-perfluorinated-surfactants-in-soils
#17
Denis Courtier-Murias, Eric Michel, Stéphane Rodts, François Lafolie
Soil contamination is still poorly understood and modeled in part because of the difficulties of looking inside the "black box" constituted by soils. Here, we investigated the application of a recently developed (1)H NMR technique to (19)F NMR relaxometry experiments and utilized the results as inputs for an existing model. This novel approach yields (19)F T2 NMR relaxation values of any fluorinated contaminant, which are among the most dangerous contaminants, allowing us to noninvasively and directly monitor their fate in soils...
February 16, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28163723/activin-a-modulates-cripto-1-hnf4%C3%AE-cells-to-guide-cardiac-differentiation-from-human-embryonic-stem-cells
#18
Robin Duelen, Guillaume Gilbert, Abdulsamie Patel, Nathalie de Schaetzen, Liesbeth De Waele, Llewelyn Roderick, Karin R Sipido, Catherine M Verfaillie, Gunnar M Buyse, Lieven Thorrez, Maurilio Sampaolesi
The use of human pluripotent stem cells in basic and translational cardiac research requires efficient differentiation protocols towards cardiomyocytes. In vitro differentiation yields heterogeneous populations of ventricular-, atrial-, and nodal-like cells hindering their potential applications in regenerative therapies. We described the effect of the growth factor Activin A during early human embryonic stem cell fate determination in cardiac differentiation. Addition of high levels of Activin A during embryoid body cardiac differentiation augmented the generation of endoderm derivatives, which in turn promoted cardiomyocyte differentiation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28154867/single-cell-laden-protease-sensitive-microniches-for-long-term-culture-in-3d
#19
Philipp S Lienemann, Torsten Rossow, Angelo S Mao, Queralt Vallmajo-Martin, Martin Ehrbar, David J Mooney
Single cell-laden three-dimensional (3D) microgels that can serve to mimic stem cell niches in vitro, and are therefore termed microniches, can be efficiently fabricated by droplet-based microfluidics. In this technique an aqueous polymer solution along with a highly diluted cell solution is injected into a microfluidic device to create monodisperse pre-microgel droplets that are then solidified by a polymer crosslinking reaction to obtain monodisperse single cell-laden microniches. However, problems limiting this approach studying the fate of single cells include Poisson encapsulation statistics that result in mostly empty microniches, and cells egressing from the microniches during subsequent cell culture...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28143895/raman-spectroscopic-imaging-of-human-macrophages-incubated-with-triglyceride-enriched-oxldl-visualizes-translocation-of-lipids-between-endocytic-vesicles-and-lipid-droplets
#20
Clara Stiebing, Lisa Schmoelz, Maria Wallert, Christian Matthaeus, Stefan Lorkowski, Juergen Popp
Raman spectroscopic imaging was used to investigate the uptake of oxidized low-density lipoproteins (oxLDL) by human macrophages. To better understand the receptor-mediated pathway and the intracellular fate of modified lipoproteins is of foremost interest with regard to the development of atherosclerotic plaques. To obtain information on the storage process of lipids caused by oxLDL uptake, Raman spectroscopic imaging was used because of its unique chemical specificity, especially for lipids. For the present study, a protocol was established to incorporate deuterated tripalmitate into oxLDL...
January 31, 2017: Journal of Lipid Research
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