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https://www.readbyqxmd.com/read/28340480/using-uhplc-q-trap-ms-as-a-complementary-technique-to-in-depth-mine-uplc-q-tof-ms-data-for-identifying-modified-nucleosides-in-urine
#1
Zhiwei Lu, Qing Wang, Meiling Wang, Shuang Fu, Qingqing Zhang, Zhixin Zhang, Huizhen Zhao, Yuehong Liu, Zhenhai Huang, Ziye Xie, Honghong Yu, Xiaoyan Gao
Modified nucleosides, metabolites of RNA, are potential biomarkers of cancer before the appearance of morphological abnormalities. It is of great significance to comprehensively detect and identify nucleosides in human urine for discovery of cancer biomarkers. However, the lower abundance, the greater polarity and the matrix effects make it difficult to detect urinary nucleosides. In this paper, an integrated method consisted of sample preparation followed by ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS) detection and primary identification, then ultra-high performance liquid chromatography coupled with hybrid triple quadrupole linear ion trap mass spectrometer (UHPLC Q-Trap/MS) further identification and validation were introduced...
March 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28340471/chemotaxonomic-studies-of-nine-paris-species-from-china-based-on-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-and-fourier-transform-infrared-spectroscopy
#2
Yuanzhong Wang, Ehu Liu, Ping Li
Paris species, which contain steroid saponins, have been used as herb folk medicines in Asia. In the present study, a comprehensive strategy based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) and Fourier transform infrared (FT-IR) spectroscopy was firstly proposed to evaluate the chemotaxonomic relationships of nine Paris species sampled from different geographical regions in China. Principle component analysis (PCA) based on FT-IR data revealed chemical similarities in term of the nine species and geographical regions, indicating the accumulation of metabolites affected by the combination of geographical factors and species...
March 18, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28339762/maternal-and-genetic-effects-on-broiler-bone-properties-during-incubation-period
#3
R Yair, A Cahaner, Z Uni, R Shahar
In order to examine the differences in bone properties between fast-growing and slow-growing broiler embryos and to understand the effects of genotype and egg size on these differences, fast- and slow-growing hens and males were reciprocally crossed to create 4 egg groups: FST (laid by fast-growing hens, inseminated by fast-growing males), H-FST (fast-growing hens and slow-growing males), H-SLW (slow-growing hens and fast-growing males), and SLW (slow-growing hens and slow-growing males). Embryos (n = 8) from these 4 groups were sacrificed and weighed, and both tibiae were harvested on embryonic d (E) 17, 19, and 21...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339458/rapid-surveillance-for-vector-presence-rsvp-development-of-a-novel-system-for-detecting-aedes-aegypti-and-aedes-albopictus
#4
Brian L Montgomery, Martin A Shivas, Sonja Hall-Mendelin, Jim Edwards, Nicholas A Hamilton, Cassie C Jansen, Jamie L McMahon, David Warrilow, Andrew F van den Hurk
BACKGROUND: The globally important Zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, Aedes aegypti and Aedes albopictus. In Australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. We describe the development of a Rapid Surveillance for Vector Presence (RSVP) system to expedite presence- absence surveys for both species. METHODOLOGY/PRINCIPAL FINDINGS: We developed a methodology that uses molecular assays to efficiently screen pooled ovitrap (egg trap) samples for traces of target species ribosomal RNA...
March 24, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28339032/clc-7-ostm1-contribute-to-the-ability-of-tea-polyphenols-to-maintain-bone-homeostasis-in-c57bl-6-mice-protecting-against-fluorosis
#5
Bing-Yun Li, Yan-Hui Gao, Jun-Rui Pei, Yan-Mei Yang, Wei Zhang, Dian-Jun Sun
Epidemiological investigations indicate that certain ingredients in tea bricks can antagonize the adverse effects of fluoride. Tea polyphenols (TPs), the most bioactive ingredient in tea bricks, have been demonstrated to be potent bone-supporting agents. ClC‑7 is known to be crucial for osteoclast (OC) bone resorption. Thus, in this study, we investigated the potential protective effects of TPs against fluorosis using a mouse model and explored the underlying mechanisms with particular focus on ClC‑7...
March 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28338605/comparing-species-composition-of-passive-trapping-of-adult-flies-with-larval-collections-from-the-body-during-scene-based-medicolegal-death-investigations
#6
Michelle R Sanford
Collection of insects at the scene is one of the most important aspects of forensic entomology and proper collection is one of the biggest challenges for any investigator. Adult flies are highly mobile and ubiquitous at scenes, yet their link to the body and the time of colonization (TOC) and post-mortem interval (PMI) estimates is not well established. Collection of adults is widely recommended for casework but has yet to be rigorously evaluated during medicolegal death investigations for its value to the investigation...
March 24, 2017: Insects
https://www.readbyqxmd.com/read/28338282/don-t-forget-to-look-down%C3%A2-%C3%A2-collaborative-approaches-to-predator-conservation
#7
Steve M Redpath, John D C Linnell, Marco Festa-Bianchet, Luigi Boitani, Nils Bunnefeld, Amy Dickman, R J Gutiérrez, R J Irvine, Maria Johansson, Aleksandra Majić, Barry J McMahon, Simon Pooley, Camilla Sandström, Annelie Sjölander-Lindqvist, Ketil Skogen, Jon E Swenson, Arie Trouwborst, Juliette Young, E J Milner-Gulland
Finding effective ways of conserving large carnivores is widely recognised as a priority in conservation. However, there is disagreement about the most effective way to do this, with some favouring top-down 'command and control' approaches and others favouring collaboration. Arguments for coercive top-down approaches have been presented elsewhere; here we present arguments for collaboration. In many parts of the developed world, flexibility of approach is built into the legislation, so that conservation objectives are balanced with other legitimate goals...
March 24, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28338010/brilliant-petawatt-gamma-ray-pulse-generation-in-quantum-electrodynamic-laser-plasma-interaction
#8
H X Chang, B Qiao, T W Huang, Z Xu, C T Zhou, Y Q Gu, X Q Yan, M Zepf, X T He
We show a new resonance acceleration scheme for generating ultradense relativistic electron bunches in helical motions and hence emitting brilliant vortical γ-ray pulses in the quantum electrodynamic (QED) regime of circularly-polarized (CP) laser-plasma interactions. Here the combined effects of the radiation reaction recoil force and the self-generated magnetic fields result in not only trapping of a great amount of electrons in laser-produced plasma channel, but also significant broadening of the resonance bandwidth between laser frequency and that of electron betatron oscillation in the channel, which eventually leads to formation of the ultradense electron bunch under resonant helical motion in CP laser fields...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337979/monolayer-optical-memory-cells-based-on-artificial-trap-mediated-charge-storage-and-release
#9
Juwon Lee, Sangyeon Pak, Young-Woo Lee, Yuljae Cho, John Hong, Paul Giraud, Hyeon Suk Shin, Stephen M Morris, Jung Inn Sohn, SeungNam Cha, Jong Min Kim
Monolayer transition metal dichalcogenides are considered to be promising candidates for flexible and transparent optoelectronics applications due to their direct bandgap and strong light-matter interactions. Although several monolayer-based photodetectors have been demonstrated, single-layered optical memory devices suitable for high-quality image sensing have received little attention. Here we report a concept for monolayer MoS2 optoelectronic memory devices using artificially-structured charge trap layers through the functionalization of the monolayer/dielectric interfaces, leading to localized electronic states that serve as a basis for electrically-induced charge trapping and optically-mediated charge release...
March 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28337204/phenol-soluble-modulin-%C3%AE-peptide-toxins-from-aggressive-staphylococcus-aureus-induce-rapid-formation-of-neutrophil-extracellular-traps-through-a-reactive-oxygen-species-independent-pathway
#10
Halla Björnsdottir, Agnes Dahlstrand Rudin, Felix P Klose, Jonas Elmwall, Amanda Welin, Marios Stylianou, Karin Christenson, Constantin F Urban, Huamei Forsman, Claes Dahlgren, Anna Karlsson, Johan Bylund
Neutrophils have the ability to capture and kill microbes extracellularly through the formation of neutrophil extracellular traps (NETs). These are DNA and protein structures that neutrophils release extracellularly and are believed to function as a defense mechanism against microbes. The classic NET formation process, triggered by, e.g., bacteria, fungi, or by direct stimulation of protein kinase C through phorbol myristate acetate, is an active process that takes several hours and relies on the production of reactive oxygen species (ROS) that are further modified by myeloperoxidase (MPO)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28336851/nano-photonic-structures-for-light-trapping-in-ultra-thin-crystalline-silicon-solar-cells
#11
Prathap Pathi, Akshit Peer, Rana Biswas
Thick wafer-silicon is the dominant solar cell technology. It is of great interest to develop ultra-thin solar cells that can reduce materials usage, but still achieve acceptable performance and high solar absorption. Accordingly, we developed a highly absorbing ultra-thin crystalline Si based solar cell architecture using periodically patterned front and rear dielectric nanocone arrays which provide enhanced light trapping. The rear nanocones are embedded in a silver back reflector. In contrast to previous approaches, we utilize dielectric photonic crystals with a completely flat silicon absorber layer, providing expected high electronic quality and low carrier recombination...
January 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28336490/novel-therapeutic-interventions-for-pseudoachondroplasia
#12
Karen L Posey, Jacqueline T Hecht
Pseudoachondroplasia (PSACH), a severe short-limbed dwarfing condition, is associated with life-long joint pain and early onset osteoarthritis. PSACH is caused by mutations in cartilage oligomeric matrix protein (COMP), a pentameric matricellular protein expressed primarily in cartilage and other musculoskeletal tissues. Mutations in COMP diminish calcium binding and as a result perturb protein folding and export to the extracellular matrix. Mutant COMP is retained in the endoplasmic reticulum (ER) of growth plate chondrocytes resulting in massive intracellular COMP retention...
March 21, 2017: Bone
https://www.readbyqxmd.com/read/28336052/does-reef-structure-affect-oyster-food-resources-a-stable-isotope-assessment
#13
Brittany N Blomberg, Benoit Lebreton, Terence A Palmer, Gaël Guillou, Jennifer Beseres Pollack, Paul A Montagna
As ecosystem engineers, oysters create and maintain structured habitat and can influence trophodynamics and benthic-pelagic coupling in the surrounding landscape. The physical reef structure and associated biotic parameters can affect the availability of food resources for oysters. Oysters and potential composite food sources - suspended particulate organic matter (SPOM) and surface sediment organic matter (SSOM) - were assessed using a dual stable isotope (δ(13)C, δ(15)N) approach at three reef types (natural, restored, and unconsolidated) seasonally for two years to determine if changes in physical and/or biotic parameters affected the relative availability and/or use of food resources by oysters...
March 11, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28335757/sophorae-flos-extract-inhibits-rankl-induced-osteoclast-differentiation-by-suppressing-the-nf-%C3%AE%C2%BAb-nfatc1-pathway-in-mouse-bone-marrow-cells
#14
Jeong-Mi Kim, Jung-Han Lee, Guem-San Lee, Eun-Mi Noh, Hyun-Kyung Song, Dong Ryun Gu, Seong-Cheol Kim, Seoung Hoon Lee, Kang-Beom Kwon, Young-Rae Lee
BACKGROUND: Sophorae Flos (SF) is a composite of flowers and buds of Styphnolobium japonicum (L.) Schott and has been used in traditional Korean and Chinese medicine for the treatment of hemostasis and inflammation. Previous studies reported that SF possesses anti-obesity properties, as well as anti-allergic, anti-proliferative, and anti-inflammatory activities. However, the effect of SF in bone resorption has not been studies. In this study, we examined the potential of SF extract (SFE) to inhibit receptor activator of NF-κB ligand (RANKL) -induced osteoclast differentiation in cultured mouse-derived bone marrow macrophages (BMMs)...
March 23, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28335456/mosquito-traps-an-innovative-environmentally-friendly-technique-to-control-mosquitoes
#15
Brigitte Poulin, Gaëtan Lefebvre, Camille Muranyi-Kovacs, Samuel Hilaire
We tested the use of mosquito traps as an alternative to spraying insecticide in Camargue (France) following the significant impacts observed on the non-target fauna through Bti persistence and trophic perturbations. In a village of 600 inhabitants, 16 Techno Bam traps emitting CO₂ and using octenol lures were set from April to November 2016. Trap performance was estimated at 70% overall based on mosquitoes landing on human bait in areas with and without traps. The reduction of Ochlerotatus caspius and Oc...
March 18, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28335368/shape-evolution-of-hierarchical-w18o49-nanostructures-a-systematic-investigation-of-the-growth-mechanism-properties-and-morphology-dependent-photocatalytic-activities
#16
Guojuan Hai, Jianfeng Huang, Liyun Cao, Yanni Jie, Jiayin Li, Xing Wang
Hierarchical tungsten oxide assemblies such as spindle-like structures, flowers with sharp petals, nanowires and regular hexagonal structures are successfully synthesized via a solvothermal reduction method by simply adjusting the reaction conditions. On the basis of the experimental results, it is determined that the reaction time significantly influences the phase transition, microstructure and photocatalytic activity of the prepared samples. The possible mechanisms for the morphology evolution process have been systematically proposed...
December 14, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335362/the-change-of-electronic-transport-behaviors-by-p-and-b-doping-in-nano-crystalline-silicon-films-with-very-high-conductivities
#17
Dan Shan, Mingqing Qian, Yang Ji, Xiaofan Jiang, Jun Xu, Kunji Chen
Nano-crystalline Si films with high conductivities are highly desired in order to develop the new generation of nano-devices. Here, we first demonstrate that the grain boundaries played an important role in the carrier transport process in un-doped nano-crystalline Si films as revealed by the temperature-dependent Hall measurements. The potential barrier height can be well estimated from the experimental results, which is in good agreement with the proposed model. Then, by introducing P and B doping, it is found that the scattering of grain boundaries can be significantly suppressed and the Hall mobility is monotonously decreased with the temperature both in P- and B-doped nano-crystalline Si films, which can be attributed to the trapping of P and B dopants in the grain boundary regions to reduce the barriers...
December 3, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335360/super-hydrophobic-icephobic-coatings-based-on-silica-nanoparticles-modified-by-self-assembled-monolayers
#18
Junpeng Liu, Zaid A Janjua, Martin Roe, Fang Xu, Barbara Turnbull, Kwing-So Choi, Xianghui Hou
A super-hydrophobic surface has been obtained from nanocomposite materials based on silica nanoparticles and self-assembled monolayers of 1H,1H,2H,2H-perfluorooctyltriethoxysilane (POTS) using spin coating and chemical vapor deposition methods. Scanning electron microscope images reveal the porous structure of the silica nanoparticles, which can trap small-scale air pockets. An average water contact angle of 163° and bouncing off of incoming water droplets suggest that a super-hydrophobic surface has been obtained based on the silica nanoparticles and POTS coating...
December 2, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335283/electrically-tunable-absorption-enhancement-with-spectral-and-polarization-selectivity-through-graphene-plasmonic-light-trapping
#19
Wenbin Liu, Jianfa Zhang, Zhihong Zhu, Xiaodong Yuan, Shiqiao Qin
In this paper, anisotropic graphene plasmonic structures are explored for light trapping and absorption enhancement in surrounding media. It is shown that electrically tunable and versatile spectral and polarization selectivity can be realized. Particularly, it is possible to control absorption of the incident light's polarization component at a specific wavelength by varying the Fermi energy with suitable geometric designs. It may find applications for new types of infrared and THz photodetectors and will promote the research of other novel polarization devices...
August 23, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335223/ultraviolet-plasmonic-aluminium-nanoparticles-for-highly-efficient-light-incoupling-on-silicon-solar-cells
#20
Yinan Zhang, Boyuan Cai, Baohua Jia
Plasmonic metal nanoparticles supporting localized surface plasmon resonances have attracted a great deal of interest in boosting the light absorption in solar cells. Among the various plasmonic materials, the aluminium nanoparticles recently have become a rising star due to their unique ultraviolet plasmonic resonances, low cost, earth-abundance and high compatibility with the complementary metal-oxide semiconductor (CMOS) manufacturing process. Here, we report some key factors that determine the light incoupling of aluminium nanoparticles located on the front side of silicon solar cells...
May 24, 2016: Nanomaterials
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