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https://www.readbyqxmd.com/read/28924033/rgpf-is-required-for-maintenance-of-stress-tolerance-and-virulence-in-streptococcus-mutans
#1
C J Kovacs, R C Faustoferri, R G Quivey
Bacterial cell wall dynamics have been implicated as important determinants of cellular physiology, stress tolerance and virulence. In Streptococcus mutans, the cell wall is composed primarily of a rhamnose-glucose polysaccharide (RGP) linked to the peptidoglycan. Despite extensive studies describing its formation and composition, the potential roles for RGP in S. mutans biology have not been well investigated. The present study characterizes the impact of RGP disruption as a result of deletion of rgpF, the gene encoding a rhamnosyltransferase involved in construction of the core poly-rhamnose backbone of RGP...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28920922/g%C3%AE-13-ablation-reprograms-myofibers-to-oxidative-phenotype-and-enhances-whole-body-metabolism
#2
Ja Hyun Koo, Tae Hyun Kim, Shi-Young Park, Min Sung Joo, Chang Yeob Han, Cheol Soo Choi, Sang Geon Kim
Skeletal muscle is a key organ in energy homeostasis owing to its high requirement for nutrients. Heterotrimeric G proteins converge signals from cell-surface receptors to potentiate or blunt responses against environmental changes. Here, we show that muscle-specific ablation of Gα13 in mice promotes reprogramming of myofibers to the oxidative type, with resultant increases in mitochondrial biogenesis and cellular respiration. Mechanistically, Gα13 and its downstream effector RhoA suppressed nuclear factor of activated T cells 1 (NFATc1), a chief regulator of myofiber conversion, by increasing Rho-associated kinase 2-mediated (Rock2-mediated) phosphorylation at Ser243...
September 18, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28920187/sub-ablative-er-cr-ysgg-laser-irradiation-under-all-ceramic-restorations-effects-on-demineralization-and-shear-bond-strength
#3
Serdar Bağlar
This study evaluated the caries resistant effects of sub-ablative Er,Cr:YSGG laser irradiation alone and combined with fluoride in comparison with fluoride application alone on enamel prepared for veneer restorations. And also, evaluated these treatments' effects on the shear bond strength of all-ceramic veneer restorations. One hundred and thirty-five human maxillary central teeth were assigned to groups of 1a-control, 1b-laser treated, 1c-fluoride treated, 1d-laser + fluoride treated for shear bond testing and to groups of 2a-positive control(non-demineralised), 2b-laser treated, 2c-fluoride treated, 2d-laser + fluoride treated, 2e-negative control (demineralised) for microhardness testing (n = 15, N = 135)...
September 17, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28918299/the-prosthetic-rehabilitation-of-a-patient-with-a-lateral-postsurgical-defect-using-a-2-piece-magnet-retained-facial-prosthesis-a-clinical-report
#4
Kariem M Elhelow, Yasser E Al-Thobaiti, Abdurabu A Gomawi
Nonsurgical prosthetic interventions are often proposed after repeated grafts have failed to restore irradiated facial defects. This report describes a facial prosthetic reconstruction following ablative surgery for a sizable facial tumor by using a novel custom-made magnet-bearing substructure, which connected with an exposed part of the mandibular reconstruction plate. The defect involved the entire left lower part of the face and angle of the mentum. This reconstructive technique, together with an added surface texture with hair-like silicone strands, effectively addressed the cosmetic needs of the patient...
September 14, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28917809/characterization-of-sodium-tripolyphosphate-and-sodium-citrate-dehydrate-residues-on-surfaces
#5
M Sadi Gurses, Can Erkey, Seda Kizilel, Alper Uzun
Sodium tripolyphosphate (STPP) and sodium citrate dihydrate (sodium citrate) are the most widely used components in detergent formulations. Here, we characterized these two components on glass surfaces to assess their possible exposures from white spots on dishwasher-washed dishes. Ultraviolet/visible near infrared spectroscopy (UV/Vis-NIR), Fourier transform infrared spectroscopy (FTIR) in the attenuated total reflectance mode (ATR-FTIR), Raman spectroscopy and laser ablation-inductively coupled plasma mass spectroscopy (LA-ICP-MS) were utilized to design calibration models for a range of STPP and sodium citrate concentrations 1-8% w/w) precipitated on glass surfaces...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28904836/laser-processing-of-thin-film-multilayer-structures-comparison-between-a-3d-thermal-model-and-experimental-results
#6
Babak B Naghshine, Amirkianoosh Kiani
In this research, a numerical model is introduced for simulation of laser processing of thin film multilayer structures, to predict the temperature and ablated area for a set of laser parameters including average power and repetition rate. Different thin-films on Si substrate were processed by nanosecond Nd:YAG laser pulses and the experimental and numerical results were compared to each other. The results show that applying a thin film on the surface can completely change the temperature field and vary the shape of the heat affected zone...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28903465/role-of-host-cell-integrins-in-the-microsporidium-encephalitozoon-intestinalis-adherence-and-infection-in-vitro
#7
Cory A Leonard, J Russell Hayman
Microsporidia are obligate intracellular, spore-forming, fungal-related pathogens that employ a unique organelle, the polar tube, to transfer infectious spore contents into host cells to initiate infection. Spore adherence to host cells may provide the proximity required for polar tube/host cell interaction during in vivo infection. In previous in vitro studies, host sulfated glycosaminoglycans (GAGs) or recombinant microsporidia endospore protein (EnP1) was implicated in the pathogen adherence and infection process; however, complete ablation of spore adherence and infection could not be achieved, suggesting that additional or alternative spore and host cell determinants of adherence and infection may exist...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28894117/preparation-of-monolayer-mos2-quantum-dots-using-temporally-shaped-femtosecond-laser-ablation-of-bulk-mos2-targets-in-water
#8
Bo Li, Lan Jiang, Xin Li, Peng Ran, Pei Zuo, Andong Wang, Liangti Qu, Yang Zhao, Zhihua Cheng, Yongfeng Lu
Zero-dimensional MoS2 quantum dots (QDs) possess distinct physical and chemical properties, which have garnered them considerable attention and facilitates their use in a broad range of applications. In this study, we prepared monolayer MoS2 QDs using temporally shaped femtosecond laser ablation of bulk MoS2 targets in water. The morphology, crystal structures, chemical, and optical properties of the MoS2 QDs were characterized by transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, UV-vis absorption spectra, and photoluminescence spectra...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28892049/membrane-mucin-muc4-promotes-blood-cell-association-with-tumor-cells-and-mediates-efficient-metastasis-in-a-mouse-model-of-breast-cancer
#9
A R Rowson-Hodel, J H Wald, J Hatakeyama, W K O'Neal, J R Stonebraker, K VanderVorst, M J Saldana, A D Borowsky, C Sweeney, K L Carraway
Mucin-4 (Muc4) is a large cell surface glycoprotein implicated in the protection and lubrication of epithelial structures. Previous studies suggest that aberrantly expressed Muc4 can influence the adhesiveness, proliferation, viability and invasiveness of cultured tumor cells, as well as the growth rate and metastatic efficiency of xenografted tumors. Although it has been suggested that one of the major mechanisms by which Muc4 potentiates tumor progression is via its engagement of the ErbB2/HER2 receptor tyrosine kinase, other mechanisms exist and remain to be delineated...
September 11, 2017: Oncogene
https://www.readbyqxmd.com/read/28887362/resolving-bipolar-electrogram-voltages-during-atrial-fibrillation-using-omnipolar-mapping
#10
Shouvik K Haldar, Karl Magtibay, Andreu Porta-Sanchez, Stéphane Massé, Nicholas Mitsakakis, Patrick F H Lai, Mohammed Ali Azam, John Asta, Marjan Kusha, Paul Dorian, Andrew C T Ha, Vijay Chauhan, D Curtis Deno, Kumaraswamy Nanthakumar
BACKGROUND: Low-voltage-guided substrate modification is an emerging strategy in atrial fibrillation (AF) ablation. A major limitation to contemporary bipolar electrogram (EGM) analysis in AF is the resultant lower peak-to-peak voltage (Vpp) from variations in wavefront direction relative to electrode orientation and from fractionation and collision events. We aim to compare bipole Vpp with novel omnipolar peak-to-peak voltages (Vmax) in sinus rhythm (SR) and AF. METHODS AND RESULTS: A high-density fixed multielectrode plaque was placed on the epicardial surface of the left atrium in dogs...
September 2017: Circulation. Arrhythmia and Electrophysiology
https://www.readbyqxmd.com/read/28887361/technical-considerations-on-phase-mapping-for-identification-of-atrial-reentrant-activity-in-direct-and-inverse-computed-electrograms
#11
Miguel Rodrigo, Andreu M Climent, Alejandro Liberos, Francisco Fernández-Avilés, Omer Berenfeld, Felipe Atienza, Maria S Guillem
BACKGROUND: Phase mapping has become a broadly used technique to identify atrial reentrant circuits for ablative therapy guidance. This work studies the phase mapping process and how the signal nature and its filtering affect the reentrant pattern characterization in electrogram (EGM), body surface potential mapping, and electrocardiographic imaging signals. METHODS AND RESULTS: EGM, body surface potential mapping, and electrocardiographic imaging phase maps were obtained from 17 simulations of atrial fibrillation, atrial flutter, and focal atrial tachycardia...
September 2017: Circulation. Arrhythmia and Electrophysiology
https://www.readbyqxmd.com/read/28884923/low-voltage-areas-detected-by-high-density-electroanatomical-mapping-predict-recurrence-after-ablation-for-paroxysmal-atrial-fibrillation
#12
Konstantinos Vlachos, Michael Efremidis, Konstantinos P Letsas, George Bazoukis, Ruairidh Martin, Maria Kalafateli, Louiza Lioni, Stamatis Georgopoulos, Athanasios Saplaouras, Theodore Efremidis, Tong Liu, Kosmas Valkanas, Nikolaos Karamichalakis, Dimitrios Asvestas, Antonios Sideris
INTRODUCTION: We aimed to evaluate the extent of atrial fibrosis in paroxysmal atrial fibrillation (AF) and the correlation with ablation outcomes after pulmonary vein antral isolation (PVΑI) using a mapping system with high-resolution and high-spatial sampling. METHODS AND RESULTS: We prospectively enrolled 80 consecutive patients (45 males, median age 60.26 years) with symptomatic paroxysmal AF who were scheduled for PVAI. Prior to PVAI, high-density bipolar voltage mapping (median number of 2,485 points) was carried out during sinus rhythm in all patients...
September 8, 2017: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/28884710/atmospheric-pulsed-laser-deposition-and-thermal-annealing-of-plasmonic-silver-nanoparticle-films
#13
Taj M Khan, Mubarak M Mujawar, Katarzyna E Siewerska, Anuj Pokle, Tony Donnelly, Niall McEvoy, Georg S Duesberg, James G Lunney
A new method for pulsed laser deposition of plasmonic silver nanoparticle films in flowing gas at atmospheric pressure is described. The ablation was done using an excimer laser at 248 nm. Fast optical imaging shows that the ablation plume is captured by the flowing gas, and is expected to form a nanoparticle aerosol, which is carried 5 - 20 mm to the substrate. The dependence of the deposition rate on laser fluence, gas flow velocity, and target-substrate distance was investigated using electron microscopy and absorption spectroscopy of the deposited films...
September 8, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28884368/anatomical-and-optical-properties-of-atrial-tissue-search-for-a-suitable-animal-model
#14
Narine Muselimyan, Mohammed Al Jishi, Huda Asfour, Luther Swift, Narine A Sarvazyan
The purpose of this study was to evaluate structural and optical properties of atrial tissue from common animal models and to compare it with human atria. We aimed to do this in a format that will be useful for development of better ablation tools and/or new means for visualizing atrial lesions. Human atrial tissue from clinically relevant age group was compared and contrasted with atrial tissue of large animal models commonly available for research purposes. These included pigs, sheep, dogs and cows. The presented data include area measurements of smooth atrial surface available for ablation and estimates of thickness of collagen and muscle for five different species...
September 7, 2017: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/28884296/an-open-label-prospective-observational-study-of-the-efficacy-of-bisphosphonate-therapy-for-painful-osteoid-osteoma
#15
Valérie Bousson, Tifenn Leturcq, Hang-Korng Ea, Olivier Hauger, Nadia Mehsen-Cetre, Bassam Hamzé, Caroline Parlier-Cuau, Jean-Denis Laredo, Thierry Schaeverbeke, Philippe Orcel
OBJECTIVES: To assess the efficacy of bisphosphonate therapy on bone pain in patients with osteoid osteoma (OO) (main objective), and to describe bisphosphonate-induced changes in nidus mineralisation and regional bone-marrow oedema (BMO). METHODS: A prospective, observational study was conducted from 2011 to 2014. Patients with risk factors for complications of percutaneous or surgical ablation or recurrence after ablation, were offered once monthly intravenous bisphosphonate treatment until significant pain alleviation was achieved...
September 7, 2017: European Radiology
https://www.readbyqxmd.com/read/28884216/standardized-unfold-mapping-a-technique-to-permit-left-atrial-regional-data-display-and-analysis
#16
Steven E Williams, Catalina Tobon-Gomez, Maria A Zuluaga, Henry Chubb, Constantine Butakoff, Rashed Karim, Elena Ahmed, Oscar Camara, Kawal S Rhode
PURPOSE: Left atrial arrhythmia substrate assessment can involve multiple imaging and electrical modalities, but visual analysis of data on 3D surfaces is time-consuming and suffers from limited reproducibility. Unfold maps (e.g., the left ventricular bull's eye plot) allow 2D visualization, facilitate multimodal data representation, and provide a common reference space for inter-subject comparison. The aim of this work is to develop a method for automatic representation of multimodal information on a left atrial standardized unfold map (LA-SUM)...
September 7, 2017: Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing
https://www.readbyqxmd.com/read/28883393/fas-cell-surface-death-receptor-controls-hepatic-lipid-metabolism-by-regulating-mitochondrial-function
#17
Flurin Item, Stephan Wueest, Vera Lemos, Sokrates Stein, Fabrizio C Lucchini, Rémy Denzler, Muriel C Fisser, Tenagne D Challa, Eija Pirinen, Youngsoo Kim, Silvio Hemmi, Erich Gulbins, Atan Gross, Lorraine A O'Reilly, Markus Stoffel, Johan Auwerx, Daniel Konrad
Nonalcoholic fatty liver disease is one of the most prevalent metabolic disorders and it tightly associates with obesity, type 2 diabetes, and cardiovascular disease. Reduced mitochondrial lipid oxidation contributes to hepatic fatty acid accumulation. Here, we show that the Fas cell surface death receptor (Fas/CD95/Apo-1) regulates hepatic mitochondrial metabolism. Hepatic Fas overexpression in chow-fed mice compromises fatty acid oxidation, mitochondrial respiration, and the abundance of mitochondrial respiratory complexes promoting hepatic lipid accumulation and insulin resistance...
September 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28871263/the-activity-of-the-neutral-sphingomyelinase-is-important-in-t-cell-recruitment-and-directional-migration
#18
Lena Collenburg, Niklas Beyersdorf, Teresa Wiese, Christoph Arenz, Essa M Saied, Katrin Anne Becker-Flegler, Sibylle Schneider-Schaulies, Elita Avota
Breakdown of sphingomyelin as catalyzed by the activity of sphingomyelinases profoundly affects biophysical properties of cellular membranes which is particularly important with regard to compartmentalization of surface receptors and their signaling relay. As it is activated both upon TCR ligation and co-stimulation in a spatiotemporally controlled manner, the neutral sphingomyelinase (NSM) has proven to be important in T cell activation, where it appears to play a particularly important role in cytoskeletal reorganization and cell polarization...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28869433/treatment-techniques-on-biocompatible-titanium-to-modify-the-surface-wetting%C3%A2-properties
#19
L Torrisi, C Scolaro
The physical properties of biocompatible titanium surfaces were modified using different techniques of surface treatment. Particularly the measurements of roughness and wetting ability were controlled using six different techniques: polishing, sandblasting, acid attack, laser ablation, ion implantation and nanoparticle deposition. The titanium surface wetting can be modified drastically depending on the used treatment to enhance the hydrophilic or the hydrophobic behaviour of the metallic biocompatible surface...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28867478/hemidesmosomal-linker-proteins-regulate-cell-motility-invasion-and-tumorigenicity-in-oral-squamous-cell-carcinoma-derived-cells
#20
Pratik Rajeev Chaudhari, Silvania Emlit Charles, Zinia Charlotte D'Souza, Milind Murlidhar Vaidya
BPAG1e and Plectin are hemidesmosomal linker proteins which anchor intermediate filament proteins to the cell surface through β4 integrin. Recent reports indicate that these proteins play a role in various cellular processes apart from their known anchoring function. However, the available literature is inconsistent. Further, the previous study from our laboratory suggested that Keratin8/18 pair promotes cell motility and tumor progression by deregulating β4 integrin signaling in oral squamous cell carcinoma (OSCC) derived cells...
August 31, 2017: Experimental Cell Research
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