keyword
https://read.qxmd.com/read/38501596/mechanosensitive-channel-mscl-gating-transitions-coupling-with-constriction-point-shift
#1
JOURNAL ARTICLE
Mingfeng Zhang, Siyang Tang, Xiaomin Wang, Sanhua Fang, Yuezhou Li
The mechanosensitive channel of large conductance (MscL) acts as an "emergency release valve" that protects bacterial cells from acute hypoosmotic stress, and it serves as a paradigm for studying the mechanism underlying the transduction of mechanical forces. MscL gating is proposed to initiate with an expansion without opening, followed by subsequent pore opening via a number of intermediate substates, and ends in a full opening. However, the details of gating process are still largely unknown. Using in vivo viability assay, single channel patch clamp recording, cysteine cross-linking, and tryptophan fluorescence quenching approach, we identified and characterized MscL mutants with different occupancies of constriction region in the pore domain...
April 2024: Protein Science
https://read.qxmd.com/read/38040172/identifying-the-mitochondrial-metabolism-network-by-integration-of-machine-learning-and-explainable-artificial-intelligence-in-skeletal-muscle-in-type-2-diabetes
#2
JOURNAL ARTICLE
Kritika Sharma, Neeru Saini, Yasha Hasija
Imbalance in glucose metabolism and insulin resistance are two primary features of type 2 diabetes/diabetes mellitus. Its etiology is linked to mitochondrial dysfunction in skeletal muscle tissue. The mitochondria are vital organelles involved in ATP synthesis and metabolism. The underlying biological pathways leading to mitochondrial dysfunction in type 2 diabetes can help us understand the pathophysiology of the disease. In this study, the mitochondrial gene expression dataset were retrieved from the GSE22309, GSE25462, and GSE18732 using Mitocarta 3...
November 29, 2023: Mitochondrion
https://read.qxmd.com/read/37992574/euphorbia-factor-l1-inhibited-transport-channel-and-energy-metabolism-in-human-colon-adenocarcinoma-cell-line-caco-2
#3
JOURNAL ARTICLE
Xiaoying Chen, Hong Hu, Xiaohuang Lin, Mengting Chen, Wenqiang Bao, Yajiao Wu, Chutao Li, Yadong Gao, Shaozhang Hou, Qiaomei Yang, Li Chen, Jian Zhang, Kunqi Chen, Qi Wang, An Zhu
Euphorbia factor L1 (EFL1) is a kind of lathyrane-type diterpenoid and is isolated from the medical herb Euphorbia lathyris L. (Euphorbiaceae); it has been reported with the toxicity that causes intestinal irritation, but the underlying mechanisms are still obscure. The objective of this study was to assess the EFL1-induced intestinal cytotoxicity in human colon adenocarcinoma Caco-2 cells. The Caco-2 cells were treated with EFL1, and the intracellular calcium ion concentration, mitochondrial membrane potential (MMP), mitochondrial permeability transition pore (mPTP), adenosine 5'-triphosphate (ATP) content, ATPase activities, TGF-β1 concentration, and transepithelial electrical resistance (TEER) were detected...
November 21, 2023: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/37878040/synthesis-structural-and-x-ray-analysis-evaluations-and-computational-studies-of-newly-tetrahydroisoquinoline-derivatives-as-potent-against-microsomal-prostaglandin-e-synthase-1
#4
JOURNAL ARTICLE
Shaaban K Mohamed, Subramani Karthikeyan, Esraa Khamies, Atazaz Ahsin, Etify Bakhite, Islam S Marae, Talaat I El-Emary, Joel T Mague, Awad I Said, Rashad Al-Salahi, Youness El Bakri
Tetrahydroisoquinolines (THIQs) are a significant class within the broad range of natural compounds known as isoquinoline alkaloids. Natural and manmade drugs based on THIQ have a variety of biological effects that protect against different infectious pathogens and neurological diseases. In this study, two new THIQ derivatives were synthesized and characterized using by X-ray crystallographic analysis. The performed Hirshfeld analysis shows the intermolecular interactions and reactive sites of compounds. The 2D fingerprints reveal dominants H···C interactions up to 8...
October 25, 2023: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/37644186/a-sporadic-parkinson-s-disease-model-via-silencing-of-the-ubiquitin-proteasome-e3-ligase-component-%C3%A2-skp1a
#5
REVIEW
Tali Fishman-Jacob, Moussa B H Youdim
Our and other's laboratory microarray-derived transcriptomic studies in human PD substantia nigra pars compacta (SNpc) samples have opened an avenue to concentrate on potential gene intersections or cross-talks along the dopaminergic (DAergic) neurodegenerative cascade in sporadic PD (SPD). One emerging gene candidate identified was SKP1A (p19, S-phase kinase-associated protein 1A), found significantly decreased in the SNpc as confirmed later at the protein level. SKP1 is part of the Skp1, Cullin 1, F-box protein (SCF) complex, the largest known class of sophisticated ubiquitin-proteasome/E3-ligases and was found to directly interact with FBXO7, a gene defective in PARK15-linked PD...
August 29, 2023: Journal of Neural Transmission
https://read.qxmd.com/read/37201586/vbp1-negatively-regulates-chip-and-selectively-inhibits-the-activity-of-hypoxia-inducible-factor-hif-1%C3%AE-but-not-hif-2%C3%AE
#6
JOURNAL ARTICLE
Yiming Yue, Yanfei Tang, Hao Huang, Dongdong Zheng, Cong Liu, Haifeng Zhang, Yunzhang Liu, Yun Li, Xiangrong Sun, Ling Lu
Hypoxia-inducible factor-1 (HIF-1) is a critical transcription factor that regulates expression of genes involved in cellular adaptation to low oxygen levels. Aberrant regulation of the HIF-1 signaling pathway is linked to various human diseases. Previous studies have established that HIF-1α is rapidly degraded in a von Hippel-Lindau protien (pVHL)-dependent manner under normoxic conditions. In this study, we find that pVHL binding protein 1 (VBP1) is a negative regulator of HIF-1α but not HIF-2α using zebrafish as an in vivo model and in vitro cell culture models...
May 16, 2023: Journal of Biological Chemistry
https://read.qxmd.com/read/36920605/the-study-of-the-strength-and-significance-of-four-biological-parameters-on-the-body-weight-of-goose
#7
JOURNAL ARTICLE
Hebatallah Abdel Moniem, Mohamed Sayed Yusuf, Ahmed Fathy, Guo-Hong Chen
Alternative products such as those from high-value protien animals have increased the demand for the production of high-quality chicken meat in past few years. This study examines the impact of two distinct feeding types on goose body-weight, as well as the genetic variation of growth hormone (GH) and pituitary-specific transcription factor (Pit-1) genes in ten goose populations using single nucleotide polymorphism (SNP) markers and PCR-RFLP analysis. Both genes were seen as very important for productivity, especially in light of the COVID-19 and its effect on poultry industry at the time...
March 15, 2023: Environmental Science and Pollution Research International
https://read.qxmd.com/read/36918965/evolution-of-tetraspanin-antigens-in-the-zoonotic-asian-blood-fluke-schistosoma-japonicum
#8
JOURNAL ARTICLE
Daniel A J Parsons, Anthony J Walker, Aidan M Emery, Joanne P Webster, Scott P Lawton
BACKGROUND: Despite successful control efforts in China over the past 60 years, zoonotic schistosomiasis caused by Schistosoma japonicum remains a threat with transmission ongoing and the risk of localised resurgences prompting calls for a novel integrated control strategy, with an anti-schistosome vaccine as a core element. Anti-schistosome vaccine development and immunisation attempts in non-human mammalian host species, intended to interrupt transmission, and utilising various antigen targets, have yielded mixed success, with some studies highlighting variation in schistosome antigen coding genes (ACGs) as possible confounders of vaccine efficacy...
March 14, 2023: Parasites & Vectors
https://read.qxmd.com/read/36534284/protein-protein-and-protein-rna-interaction-assays-to-determine-similarity-of-ini1-smarcb1-and-tar-rna-in-binding-to-hiv-1-integrase
#9
JOURNAL ARTICLE
Ganjam V Kalpana
INI1/SMARCB1 is a host protein that interacts with HIV-1 integrase (IN) and influences multiple stages of viral replication. IN is a viral enzyme responsible for integration, and it also binds to HIV-1 genomic RNA. Recent studies from our laboratory demonstrated that IN-interacting Rpt1 (Repeat 1) domain of INI1 and TAR RNA region of HIV-1 genome both bind to the same residues and surface of IN C-terminal domain (CTD). Based on a series of analyses, we found that INI1-Rpt1 and TAR RNA structurally mimic each other and that IN mutants defective for binding to INI1 are also defective for binding to RNA and produce morphologically defective virions...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/32334051/cullin-ring-ligase-5-functional-characterization-and-its-role-in-human-cancers
#10
REVIEW
Yongchao Zhao, Xiufang Xiong, Yi Sun
Cullin-RING ligase 5 (CRL5) is a multi-protein complex and consists of a scaffold protien cullin 5, a RING protein RBX2 (also known as ROC2 or SAG), adaptor proteins Elongin B/C, and a substrate receptor protein SOCS. Through targeting a variety of substrates for proteasomal degradation or modulating various protein-protein interactions, CRL5 is involved in regulation of many biological processes, such as cytokine signal transduction, inflammation, viral infection, and oncogenesis. As many substrates of CRL5 are well-known oncoproteins or tumor suppressors, abnormal regulation of CRL5 is commonly found in human cancers...
April 22, 2020: Seminars in Cancer Biology
https://read.qxmd.com/read/31701154/can-mirnas-serve-as-potential-markers-in-thermal-burn-injury-an-in-silico-approach
#11
JOURNAL ARTICLE
Sandeep K Shukla, Ajay K Sharma, Rhythm Bharti, Vidit Kulshrestha, Aman Kalonia, Priyanka Shaw
Burn injury has been a major cause of morbidity at global levels. They can occur by multiple agents like thermal, radiation, chemicals etc. Among all, thermal burn is predominant and may require specialized treatment in some patients. Although various biomarkers are reportedly used in thermal burn for understanding the pathophysiology of the injury, however their limitations prompt for search of suitable markers that can address depth and severity of the burn. MicroRNAs are conserved non-coding molecules which seem to be the promising marker due to their role in multiple pathways and participation in different physiological processes of the body...
November 8, 2019: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://read.qxmd.com/read/30900311/pirna-dq722010-contributes-to-prostate-hyperplasia-of-the-male-offspring-mice-after-the-maternal-exposed-to-microcystin-leucine-arginine
#12
JOURNAL ARTICLE
Ruitong Han, Ling Zhang, Weidong Gan, Kai Fu, Ke Jiang, Jie Ding, Jiang Wu, Xiaodong Han, Dongmei Li
BACKGROUND: Microcystin-leucine arginine (MC-LR) could disrupt prostate development and cause prostate hyperplasia. But whether and how maternal and before-weaning MC-LR exposure causes prostate hyperplasia in male offspring by changing expression profile of P-element-induced wimpy (PIWI)-interacting RNAs (piRNAs) have not yet been reported. METHODS: From the 12th day in the embryonic period to the 21st day after offspring birth, three groups of pregnant mice that were randomly assigned were exposed to 0, 10, and 50 μg/L of MC-LR through drinking water followed by the analyses of their male offspring...
March 22, 2019: Prostate
https://read.qxmd.com/read/30637830/immuno-detection-of-mrna-binding-protein-complex-in-human-cells-under-transmission-electron-microscopy
#13
JOURNAL ARTICLE
Qingfeng Ma, Takanori Tatsuno, Yuka Nakamura, Shin-Ichi Izumi, Naohisa Tomosugi, Yasuhito Ishigaki
Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA-protein complex. Visualizing the mRNA is crucial for understanding the mechanisms underlying mRNA processing and elucidating its structure and recent advances in mRNA imaging allow detection of real-time mRNA localization in living cells. However, these techniques revealed only the location of mRNA but cannot visualize the conformation of mRNA-protein complex in cells...
January 14, 2019: Microscopy Research and Technique
https://read.qxmd.com/read/24881784/brain-vascular-lesions-a-clinicopathologic-immunohistochemistry-and-ultrastructural-approach
#14
JOURNAL ARTICLE
Marisol Galván Navarrete, Alma Dalia Hernández, Miguel Angel Collado-Ortiz, Citlaltepetl Salinas-Lara, Martha Lilia Tena-Suck
Brain vascular malformations are relatively common lesions that cause serious neurologic disability or death in a significant proportion of individuals bearing them. The purpose of this study was to analyze the clinicopathologic and immunohistochemistry these lesions, looking for common antibodies expressed such as CD31, CD34, CD15, factor VIII, nestin, vimentin, vascular endothelial grow factor (VEGF), vascular endothelial grow factor receptor-2 (VEGF-R2), glial fibrillar acidic protien (GFAP), and fibroblastic grow factor β (β-FGF) and ultrastructure in endothelial cells as well as in vessel walls...
August 2014: Annals of Diagnostic Pathology
https://read.qxmd.com/read/24435274/phycobilisome-structure-and-function
#15
JOURNAL ARTICLE
B A Zilinskas, L S Greenwald
Phycobilisomes are aggregates of light-harvesting proteins attached to the stroma side of the thylakoid membranes of the cyanobacteria (blue-green algae) and red algae. The water-soluble phycobiliproteins, of which there are three major groups, tetrapyrrole chromophores covalently bound to apoprotein. Several additional protiens are found within the phycobilisome and serve to link the phycobiliproteins to each other in an ordered fashion and also to attach the phycobilisome to the thylakoid membrane. Excitation energy absorbed by phycoerythrin is transferred through phycocyanin to allophycocyanin with an efficiency approximating 100%...
January 1986: Photosynthesis Research
https://read.qxmd.com/read/23097312/growth-factor-transgenes-interactively-regulate-articular-chondrocytes
#16
JOURNAL ARTICLE
Shuiliang Shi, Scott Mercer, George J Eckert, Stephen B Trippel
Adult articular chondrocytes lack an effective repair response to correct damage from injury or osteoarthritis. Polypeptide growth factors that stimulate articular chondrocyte proliferation and cartilage matrix synthesis may augment this response. Gene transfer is a promising approach to delivering such factors. Multiple growth factor genes regulate these cell functions, but multiple growth factor gene transfer remains unexplored. We tested the hypothesis that multiple growth factor gene transfer selectively modulates articular chondrocyte proliferation and matrix synthesis...
April 2013: Journal of Cellular Biochemistry
https://read.qxmd.com/read/23096592/an-effective-system-for-detecting-protein-protein-interaction-based-on-in-vivo-cleavage-by-ppv-nia-protease
#17
JOURNAL ARTICLE
Nuoyan Zheng, Xiahe Huang, Bojiao Yin, Dan Wang, Qi Xie
Detection of protein-protein interaction can provide valuable information for investigating the biological function of proteins. The current methods that applied in protein-protein interaction, such as co-immunoprecipitation and pull down etc., often cause plenty of working time due to the burdensome cloning and purification procedures. Here we established a system that characterization of protein-protein interaction was accomplished by co-expression and simply purification of target proteins from one expression cassette within E...
December 2012: Protein & Cell
https://read.qxmd.com/read/22708623/identification-of-moesin-as-nkcc2-interacting-protein-and-analysis-of-its-functional-role-in-the-nkcc2-apical-trafficking
#18
JOURNAL ARTICLE
Monica Carmosino, Federica Rizzo, Giuseppe Procino, Lello Zolla, Anna Maria Timperio, Davide Basco, Claudia Barbieri, Silvia Torretta, Maria Svelto
BACKGROUND INFORMATION: The renal Na(+) -K(+) -2Cl(-) co-transporter (NKCC2) is expressed in kidney thick ascending limb cells, where it mediates NaCl re-absorption regulating body salt levels and blood pressure. RESULTS: In this study, we used a well-characterised NKCC2 construct (c-NKCC2) to identify NKCC2-interacting proteins by an antibody shift assay coupled with blue native/SDS-PAGE and mass spectrometry. Among the interacting proteins, we identified moesin, a protein belonging to ezrin, eadixin and moesin family...
November 2012: Biology of the Cell
https://read.qxmd.com/read/11576932/advanced-glycation-end-products-and-the-progressive-course-of-renal-disease
#19
REVIEW
A Heidland, K Sebekova, R Schinzel
In experimental and human diabetic nephropathy (DN), it has been shown that advanced glycation end products (AGEs), in particular, carboxymethyl-lysine and pentosidine, accumulate with malondialdehyde in glomerular lesions in relation to disease severity and in the presence of an upregulated receptor for AGE (RAGE) in podocytes. Toxic effects of AGEs result from structural and functional alterations in plasma and extracellular matrix (ECM) proteins, in particular, from cross-linking of proteins and interaction of AGEs with their receptors and/or binding proteins...
October 2001: American Journal of Kidney Diseases
https://read.qxmd.com/read/11279292/do-fertilin-beta-and-cyritestin-play-a-major-role-in-mammalian-sperm-oolemma-interactions-a-critical-re-evaluation-of-the-use-of-peptide-mimics-in-identifying-specific-oocyte-recognition-protiens
#20
REVIEW
E A McLaughlin, J Frayne, G Bloomerg, L Hall
Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin beta and cyritestin, to inhibit sperm--oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin beta and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach...
April 2001: Molecular Human Reproduction
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