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https://www.readbyqxmd.com/read/24881784/brain-vascular-lesions-a-clinicopathologic-immunohistochemistry-and-ultrastructural-approach
#1
Marisol Galván Navarrete, Alma Dalia Hernández, Miguel Angel Collado-Ortiz, Citlaltepetl Salinas-Lara, Martha Lilia Tena-Suck
Brain vascular malformations are relatively common lesions that cause serious neurologic disability or death in a significant proportion of individuals bearing them. The purpose of this study was to analyze the clinicopathologic and immunohistochemistry these lesions, looking for common antibodies expressed such as CD31, CD34, CD15, factor VIII, nestin, vimentin, vascular endothelial grow factor (VEGF), vascular endothelial grow factor receptor-2 (VEGF-R2), glial fibrillar acidic protien (GFAP), and fibroblastic grow factor β (β-FGF) and ultrastructure in endothelial cells as well as in vessel walls...
August 2014: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/24435274/phycobilisome-structure-and-function
#2
B A Zilinskas, L S Greenwald
Phycobilisomes are aggregates of light-harvesting proteins attached to the stroma side of the thylakoid membranes of the cyanobacteria (blue-green algae) and red algae. The water-soluble phycobiliproteins, of which there are three major groups, tetrapyrrole chromophores covalently bound to apoprotein. Several additional protiens are found within the phycobilisome and serve to link the phycobiliproteins to each other in an ordered fashion and also to attach the phycobilisome to the thylakoid membrane. Excitation energy absorbed by phycoerythrin is transferred through phycocyanin to allophycocyanin with an efficiency approximating 100%...
January 1986: Photosynthesis Research
https://www.readbyqxmd.com/read/23097312/growth-factor-transgenes-interactively-regulate-articular-chondrocytes
#3
Shuiliang Shi, Scott Mercer, George J Eckert, Stephen B Trippel
Adult articular chondrocytes lack an effective repair response to correct damage from injury or osteoarthritis. Polypeptide growth factors that stimulate articular chondrocyte proliferation and cartilage matrix synthesis may augment this response. Gene transfer is a promising approach to delivering such factors. Multiple growth factor genes regulate these cell functions, but multiple growth factor gene transfer remains unexplored. We tested the hypothesis that multiple growth factor gene transfer selectively modulates articular chondrocyte proliferation and matrix synthesis...
April 2013: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/23096592/an-effective-system-for-detecting-protein-protein-interaction-based-on-in-vivo-cleavage-by-ppv-nia-protease
#4
Nuoyan Zheng, Xiahe Huang, Bojiao Yin, Dan Wang, Qi Xie
Detection of protein-protein interaction can provide valuable information for investigating the biological function of proteins. The current methods that applied in protein-protein interaction, such as co-immunoprecipitation and pull down etc., often cause plenty of working time due to the burdensome cloning and purification procedures. Here we established a system that characterization of protein-protein interaction was accomplished by co-expression and simply purification of target proteins from one expression cassette within E...
December 2012: Protein & Cell
https://www.readbyqxmd.com/read/22708623/identification-of-moesin-as-nkcc2-interacting-protein-and-analysis-of-its-functional-role-in-the-nkcc2-apical-trafficking
#5
Monica Carmosino, Federica Rizzo, Giuseppe Procino, Lello Zolla, Anna Maria Timperio, Davide Basco, Claudia Barbieri, Silvia Torretta, Maria Svelto
BACKGROUND INFORMATION: The renal Na(+) -K(+) -2Cl(-) co-transporter (NKCC2) is expressed in kidney thick ascending limb cells, where it mediates NaCl re-absorption regulating body salt levels and blood pressure. RESULTS: In this study, we used a well-characterised NKCC2 construct (c-NKCC2) to identify NKCC2-interacting proteins by an antibody shift assay coupled with blue native/SDS-PAGE and mass spectrometry. Among the interacting proteins, we identified moesin, a protein belonging to ezrin, eadixin and moesin family...
November 2012: Biology of the Cell
https://www.readbyqxmd.com/read/11576932/advanced-glycation-end-products-and-the-progressive-course-of-renal-disease
#6
REVIEW
A Heidland, K Sebekova, R Schinzel
In experimental and human diabetic nephropathy (DN), it has been shown that advanced glycation end products (AGEs), in particular, carboxymethyl-lysine and pentosidine, accumulate with malondialdehyde in glomerular lesions in relation to disease severity and in the presence of an upregulated receptor for AGE (RAGE) in podocytes. Toxic effects of AGEs result from structural and functional alterations in plasma and extracellular matrix (ECM) proteins, in particular, from cross-linking of proteins and interaction of AGEs with their receptors and/or binding proteins...
October 2001: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
https://www.readbyqxmd.com/read/11279292/do-fertilin-beta-and-cyritestin-play-a-major-role-in-mammalian-sperm-oolemma-interactions-a-critical-re-evaluation-of-the-use-of-peptide-mimics-in-identifying-specific-oocyte-recognition-protiens
#7
REVIEW
E A McLaughlin, J Frayne, G Bloomerg, L Hall
Integrins have been proposed to play a role in mammalian sperm-oocyte interactions for many years. To a large extent this hypothesis stems from the ability of short synthetic peptides, based on the disintegrin-like domains of two sperm surface integral membrane proteins, fertilin beta and cyritestin, to inhibit sperm--oocyte binding and fusion in vitro. Here we argue that such peptide mimics lack specificity in these simple IVF assay systems. Hence, whilst not precluding a role for fertilin beta and cyritestin in sperm-oolemma interactions, this lack of specificity indicates the need for considerable caution when interpreting results obtained using this approach...
April 2001: Molecular Human Reproduction
https://www.readbyqxmd.com/read/10857599/biomolecular-interaction-analysis-mass-spectometry-bia-ms-can-detect-and-characterize-protiens-in-complex-biological-fluids-at-the-low-to-subfemtomole-level
#8
R W Nelson, D Nedelkov, K A Tubbs
No abstract text is available yet for this article.
June 1, 2000: Analytical Chemistry
https://www.readbyqxmd.com/read/1120610/alterations-in-the-metabolism-of-hamster-tracheas-in-organ-culture-after-infection-by-virulent-mycoplasma-pneumoniae
#9
P C Hu, A M Collier, J B Baseman
Exposure of hamster tracheal rings in organ culture to virulent Mycoplasma pneumoniae organisms leads to alterations in macromolecular biosynthesis and metabolic activity of the respiratory epithelial cells. Avirulent organisms derived from the same parent strain do not produce these effects. During the course of infection by virulent mycoplasmas, tracheal rings show an initial increase in [14C]galactose uptake followed by a significant decline as infection progresses which is also accompanied by abnormal processing of galactose as evidenced by amounts of 14CO2 released...
April 1975: Infection and Immunity
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