Xiaoying Chen, Hong Hu, Xiaohuang Lin, Mengting Chen, Wenqiang Bao, Yajiao Wu, Chutao Li, Yadong Gao, Shaozhang Hou, Qiaomei Yang, Li Chen, Jian Zhang, Kunqi Chen, Qi Wang, An Zhu
Euphorbia factor L1 (EFL1) is a kind of lathyrane-type diterpenoid and is isolated from the medical herb Euphorbia lathyris L. (Euphorbiaceae); it has been reported with the toxicity that causes intestinal irritation, but the underlying mechanisms are still obscure. The objective of this study was to assess the EFL1-induced intestinal cytotoxicity in human colon adenocarcinoma Caco-2 cells. The Caco-2 cells were treated with EFL1, and the intracellular calcium ion concentration, mitochondrial membrane potential (MMP), mitochondrial permeability transition pore (mPTP), adenosine 5'-triphosphate (ATP) content, ATPase activities, TGF-β1 concentration, and transepithelial electrical resistance (TEER) were detected...
November 21, 2023: Biomedicine & Pharmacotherapy