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https://www.readbyqxmd.com/read/28106508/gelma-encapsulated-hdpscs-and-huvecs-for-dental-pulp-regeneration
#1
A Khayat, N Monteiro, E E Smith, S Pagni, W Zhang, A Khademhosseini, P C Yelick
Pulpal revascularization is commonly used in the dental clinic to obtain apical closure of immature permanent teeth with thin dentinal walls. Although sometimes successful, stimulating bleeding from the periapical area of the tooth can be challenging and in turn may deleteriously affect tooth root maturation. Our objective here was to define reliable methods to regenerate pulp-like tissues in tooth root segments (RSs). G1 RSs were injected with human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) encapsulated in 5% gelatin methacrylate (GelMA) hydrogel...
February 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28098239/immunoglobulin-light-chain-igl-genes-in-torafugu-genomic-organization-and-identification-of-a-third-teleost-igl-isotype
#2
Xi Fu, Fengjun Zhang, Shugo Watabe, Shuichi Asakawa
Here, we report a genome-wide survey of immunoglobulin light chain (IGL) genes of torafugu (Takifugu rubripes) revealing multi-clusters spanning three separate chromosomes (v5 assembly) and 45 scaffolds (v4 assembly). Conventional sequence similarity searches and motif scanning approaches based on recombination signal sequence (RSS) motifs were used. We found that three IGL isotypes (L1, L2, and L3) exist in torafugu and that several loci for each isotype are present. The transcriptional orientations of the variable IGL (VL) segments were found to be either the same (in the L2 isotype) or opposite (in the L1 and L3 isotypes) to the IGL joining (JL) and constant (CL) segments, suggesting they can undergo rearrangement by deletion or inversion when expressed...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28087825/incidence-and-predictors-of-epilepsy-after-pediatric-arterial-ischemic-stroke
#3
Lori L Billinghurst, Lauren A Beslow, Nicholas S Abend, Michael Uohara, Laura Jastrzab, Daniel J Licht, Rebecca N Ichord
OBJECTIVE: To determine the cumulative incidence and clinical predictors of remote symptomatic seizures and epilepsy after pediatric arterial ischemic stroke (AIS). METHODS: We performed a retrospective analysis of 218 participants with neonatal AIS (NAIS), presumed perinatal AIS (PPAIS), and childhood AIS (CAIS) from a single-center prospective consecutive cohort enrolled from 2006 to 2014. Medical records were reviewed for timing, semiology, and treatment of acute symptomatic seizures, remote symptomatic seizures (RSS), and epilepsy...
January 13, 2017: Neurology
https://www.readbyqxmd.com/read/28068571/photocatalytic-degradation-of-clofibric-acid-by-g-c3n4-p25-composites-under-simulated-sunlight-irradiation-the-significant-effects-of-reactive-species
#4
Ping Chen, Fengliang Wang, Qianxin Zhang, Yuehan Su, Lingzhi Shen, Kun Yao, Zhi-Feng Chen, Yang Liu, Zongwei Cai, Wenying Lv, Guoguang Liu
Pharmaceutically emerging micropollutants have become an environmental concern in recent years. In the present paper, the reactive species (RSs)-induced degradation mechanism of clofibric acid (CA) was investigated using a newly sunlight-driven g-C3N4/P25 photocatalyst. A very low g-C3N4 content of 8.0 weight percent resulted in a 3.36 and a 2.29 times faster reaction rate for CA photodegradation than for pristine g-C3N4 and P25, respectively. Electron spin resonance and quenching experiments demonstrated the participation of HO, h(+), e(-), (1)O2 and O2(·-) in the photocatalytic system, and the contribution rates were calculated to 73...
January 4, 2017: Chemosphere
https://www.readbyqxmd.com/read/28065637/the-steroid-metabolite-16-%C3%AE-oh-androstenedione-generated-by-cyp21a2-serves-as-a-substrate-for-cyp19a1
#5
J Neunzig, M Milhim, L Schiffer, Y Khatri, J Zapp, A Sánchez-Guijo, M F Hartmann, S A Wudy, R Bernhardt
The 21-hydroxylase (CYP21A2) is a steroidogenic enzyme crucial for the synthesis of mineralo- and glucocorticoids. It is described to convert progesterone as well as 17-OH-progesterone, through a hydroxylation at position C21, into 11-deoxycorticosterone (DOC) and 11-deoxycortisol (RSS), respectively. In this study we unraveled CYP21A2 to have a broader steroid substrate spectrum than assumed. Utilizing a reconstituted in vitro system, consisting of purified human CYP21A2 and human cytochrome P450 reductase (CPR) we demonstrated that CYP21A2 is capable to metabolize DOC, RSS, androstenedione (A4) and testosterone (T)...
January 5, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28051989/minute-ventilation-limitations-of-two-field-transport-ventilators
#6
Dale F Szpisjak, Gregory Horn, Samuel Shalov, Alvin Angelo Abes, Lauren Van Decar
Knowledge of transport ventilator performance impacts patient safety. This study compared minute ventilation (VE) of the MOVES and Uni-Vent 731 when ventilating the VentAid Training Test Lung with compliance (C) ranging from 0.02 to 0.10 L/cm H2O and three different airway resistances (R) (none, Rp5, or Rp20). Tidal volume (VT) was 800 ± 25 mL. Respiratory rate was increased to ventilator's maximum or until auto-PEEP > 5 cm H2O. Respiratory parameters were recorded with the RSS 100HR Research Pneumotach...
January 2017: Military Medicine
https://www.readbyqxmd.com/read/28051338/activity-dependent-sulfhydration-signal-controls-nmdar-dependent-synaptic-plasticity-via-increasing-d-serine-availability
#7
Yuan-Long Li, Pei-Fei Wu, Jian-Guo Chen, Sheng Wang, Qian-Qian Han, Dan Li, Wen Wang, Xin-Lei Guan, Di Li, Li-Hong Long, Jian-Geng Huang, Fang Wang
AIMS: Reactive sulfur species (RSS) including hydrogen sulfide (H2S) and its oxydates have been raised as novel redox signaling molecules. The present study aimed at examining whether endogenous sulfhydration signal are required for long-term potentiation (LTP), a cellular model for memory. RESULTS: Here, we found that increased synaptic activity triggered sulfide generationand protein sulfhydration. Activity-triggered sulfide production was essential for N-methyl-D-aspartate subtype glutamate receptors (NMDARs)-dependent LTP via maintaining the availability of D-serine, a primary co-agonist for synaptic NMDARs...
January 4, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28048462/su-d-brb-01-a-comparison-of-learning-methods-for-knowledge-based-dose-prediction-for-coplanar-and-non-coplanar-liver-radiotherapy
#8
A Tran, D Ruan, K Woods, V Yu, D Nguyen, K Sheng
PURPOSE: The predictive power of knowledge based planning (KBP) has considerable potential in the development of automated treatment planning. Here, we examine the predictive capabilities and accuracy of previously reported KBP methods, as well as an artificial neural networks (ANN) method. Furthermore, we compare the predictive accuracy of these methods on coplanar volumetric-modulated arc therapy (VMAT) and non-coplanar 4π radiotherapy. METHODS: 30 liver SBRT patients previously treated using coplanar VMAT were selected for this study...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28044933/should-arterial-embolization-in-recurrent-spontaneous-hemophilic-hemarthroses-refractory-to-intensive-prophylaxis-be-the-first-invasive-resort
#9
E Carlos Rodriguez-Merchan
This paper reviews the role of arterial embolization (AE) in recurrent spontaneous hemarthroses (RSH) that are refractory to intensive prophylaxis (RIP) in people with hemophilia (PWH). There are six articles in the literature on the topic. The total number of AEs performed so far is 78 in 69 patients. Four second AEs were required (4/78), and a third AE in one (1/78). No serious adverse effects have been reported except for a pseudoaneurym (1/78) of the femoral artery at the puncture site (that eventually required surgical repair) and for one patient (1/78) that had recurrence of bleeding for whom surgical exploration was required...
December 16, 2016: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/28039168/correlation-of-the-rutgeerts-score-and-recurrence-of-crohn-s-disease-in-patients-with-end-ileostomy
#10
Vasutakarn Chongthammakun, Andre Fialho, Andrea Fialho, Rocio Lopez, Bo Shen
BACKGROUND: Recurrence of Crohn's disease (CD) can occur after surgery, including end ileostomy (EI). The Rutgeerts score (RS) was developed to predict postsurgical CD recurrence via ileocolonoscopy in patients having ileocolonic resection. The role of ileoscopic evaluation via stoma for assessing recurrence of CD has not been investigated. The aim of this study was to evaluate the role of ileoscopy for predicting disease recurrence in CD patients after EI with the use of RS. METHODS: A total of 73 eligible CD patients with at least two ileoscopies in our institution following EI were included...
December 30, 2016: Gastroenterology Report
https://www.readbyqxmd.com/read/28027852/analyses-of-differentially-expressed-genes-after-exposure-to-acute-stress-acute-ethanol-or-a-combination-of-both-in-mice
#11
Jessica A Baker, Jingxin Li, Diana Zhou, Ming Yang, Melloni N Cook, Byron C Jones, Megan K Mulligan, Kristin M Hamre, Lu Lu
Alcohol abuse is a complex disorder, which is confounded by other factors, including stress. In the present study, we examined gene expression in the hippocampus of BXD recombinant inbred mice after exposure to ethanol (NOE), stress (RSS), and the combination of both (RSE). Mice were given an intraperitoneal (i.p.) injection of 1.8 g/kg ethanol or saline, and subsets of both groups were exposed to acute restraint stress for 15 min or controls. Gene expression in the hippocampus was examined using microarray analysis...
December 16, 2016: Alcohol
https://www.readbyqxmd.com/read/28025530/high-performance-cmos-light-detector-with-dark-current-suppression-in-variable-temperature-systems
#12
Wen-Sheng Lin, Guo-Ming Sung, Jyun-Long Lin
This paper presents a dark current suppression technique for a light detector in a variable-temperature system. The light detector architecture comprises a photodiode for sensing the ambient light, a dark current diode for conducting dark current suppression, and a current subtractor that is embedded in the current amplifier with enhanced dark current cancellation. The measured dark current of the proposed light detector is lower than that of the epichlorohydrin photoresistor or cadmium sulphide photoresistor...
December 23, 2016: Sensors
https://www.readbyqxmd.com/read/28024270/the-stress-hormone-cortisol-blocks-perceptual-learning-in-humans
#13
Hubert R Dinse, J C Kattenstroth, M Lenz, M Tegenthoff, O T Wolf
Cortisol, the primary glucocorticoid (GC) in humans, influences neuronal excitability and plasticity by acting on mineralocorticoid and glucocorticoid receptors. Cellular studies demonstrated that elevated GC levels affect neuronal plasticity, for example through a reduction of hippocampal long-term potentiation (LTP). At the behavioural level, after treatment with GCs, numerous studies have reported impaired hippocampal function, such as impaired memory retrieval. In contrast, relatively little is known about the impact of GCs on cortical plasticity and perceptual learning in adult humans...
December 9, 2016: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28024119/accuracy-of-international-ultrasound-risk-stratification-systems-in-thyroid-lesions-cytologically-classified-as-indeterminate
#14
Pierpaolo Trimboli, Franco Fulciniti, Valentina Zilioli, Luca Ceriani, Luca Giovanella
BACKGROUND: Eminent international societies have published their thyroid ultrasound risk stratification system (US-RSS) to give a friendly tool for thyroidologists. In particular, American Thyroid Association (ATA), American Association Clinical Endocrinologists/American College of Endocrinologists and Associazione Medici Endocrinologi (AACE/ACE/AME), British Thyroid Association (BTA), and Thyroid Imaging Reporting and Data System (TIRADS) are often adopted for clinical practice. The reliability of these US-RSS has not been evaluated in discriminating cancers from benignancy in nodules with indeterminate FNA...
December 26, 2016: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28007880/residual-syntax-score-following-coronary-artery-bypass-grafting%C3%A2
#15
Giovanni Melina, Emiliano Angeloni, Simone Refice, Cristian Benegiamo, Andrea Lechiancole, Maria Matteucci, Antonino Roscitano, Roberto Bianchini, Fabio Capuano, Cosimo Comito, Pietro Spitaleri, Euclide Tonelli, Giulio Speciale, Christian Pristipino, Francesco Monti, Roberto Serdoz, Francesco Paneni, Riccardo Sinatra
OBJECTIVES: To quantify residual coronary artery disease measured using the SYNTAX score (SS) and its relation to outcomes after coronary artery bypass grafting (CABG). METHODS: We conducted a retrospective analysis on a consecutive series of 1608 patients [mean age 68 years, standard deviation (SD): 7, F:M, 242:1366] undergoing first-time isolated CABG from 2004 to 2015. The baseline SS was retrospectively determined from preoperative angiograms, and the residual SS (rSS) was measured during assessment of the actual operative report for each patient after CABG...
December 22, 2016: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/27999392/smart-pipe-system-for-a-shipyard-4-0
#16
Paula Fraga-Lamas, Diego Noceda-Davila, Tiago M Fernández-Caramés, Manuel A Díaz-Bouza, Miguel Vilar-Montesinos
As a result of the progressive implantation of the Industry 4.0 paradigm, many industries are experimenting a revolution that shipyards cannot ignore. Therefore, the application of the principles of Industry 4.0 to shipyards are leading to the creation of Shipyards 4.0. Due to this, Navantia, one of the 10 largest shipbuilders in the world, is updating its whole inner workings to keep up with the near-future challenges that a Shipyard 4.0 will have to face. Such challenges can be divided into three groups: the vertical integration of production systems, the horizontal integration of a new generation of value creation networks, and the re-engineering of the entire production chain, making changes that affect the entire life cycle of each piece of a ship...
December 20, 2016: Sensors
https://www.readbyqxmd.com/read/27998937/improving-flux-predictions-by-integrating-data-from-multiple-strains
#17
Matthew R Long, Jennifer L Reed
MOTIVATION: Incorporating experimental data into constraint-based models can improve the quality and accuracy of their metabolic flux predictions. Unfortunately, routinely and easily measured experimental data such as growth rates, extracellular fluxes, transcriptomics, and even proteomics are not always sufficient to significantly improve metabolic flux predictions. RESULTS: We developed a new method (called REPPS) for incorporating experimental measurements of growth rates and extracellular fluxes from a set of perturbed reference strains (RSs) and a parental strain (PS) to substantially improve the predicted flux distribution of the parental strain...
December 20, 2016: Bioinformatics
https://www.readbyqxmd.com/read/27997767/characterization-of-silencing-suppressor-p24-of-grapevine-leafroll-associated-virus-2
#18
Mingjun Li, Jiao Zhang, Ming Feng, Xianyou Wang, Chen Luo, Qi Wang, Yuqin Cheng
Grapevine leafroll-associated virus 2 (GLRaV-2) p24 has been reported as an RNA-silencing suppressor (RSS). However, the mechanisms underlying p24's suppression of RNA silencing are unknown. Using Agrobacterium infiltration-mediated RNA-silencing assays, we showed that GLRaV-2 p24 is a strong RSS triggered by positive-sense GFP RNA, and that silencing suppression by p24 effectively blocks accumulation of small interfering RNAs. Deletion analyses showed that the region of amino acids 1-188, which contains all predicted α-helixes and β-strands, is required for p24's RSS activity...
December 20, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27989906/advanced-diffusion-models-in-head-and-neck-squamous-cell-carcinoma-patients-goodness-of-fit-relationships-among-diffusion-parameters-and-comparison-with-dynamic-contrast-enhanced-perfusion
#19
Noriyuki Fujima, Tomohiro Sakashita, Akihiro Homma, Yukie Shimizu, Atsushi Yoshida, Taisuke Harada, Khin Khin Tha, Kohsuke Kudo, Hiroki Shirato
PURPOSE: We assessed advanced fitting models of diffusion weighted imaging (DWI) in head/neck squamous cell carcinoma (HNSCC) patients to determine the best goodness of fit and correlations among diffusion parameters. We compared these results with those of dynamic contrast-enhanced (DCE) perfusion parameters. MATERIALS AND METHODS: We retrospectively evaluated 32 HNSCC patients (12 sinonasal, 20 pharynx/oral cavity). The DWI acquisition used single-shot spin-echo echo-planar imaging (EPI) with 12 b-values (0-2000)...
October 27, 2016: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/27983670/an-indoor-positioning-method-for-smartphones-using-landmarks-and-pdr
#20
Xi Wang, Mingxing Jiang, Zhongwen Guo, Naijun Hu, Zhongwei Sun, Jing Liu
Recently location based services (LBS) have become increasingly popular in indoor environments. Among these indoor positioning techniques providing LBS, a fusion approach combining WiFi-based and pedestrian dead reckoning (PDR) techniques is drawing more and more attention of researchers. Although this fusion method performs well in some cases, it still has some limitations, such as heavy computation and inconvenience for real-time use. In this work, we study map information of a given indoor environment, analyze variations of WiFi received signal strength (RSS), define several kinds of indoor landmarks, and then utilize these landmarks to correct accumulated errors derived from PDR...
December 15, 2016: Sensors
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