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https://www.readbyqxmd.com/read/28631603/a-single-point-mutation-in-tomato-spotted-wilt-virus-nss-protein-is-sufficient-to-overcome-tsw-gene-mediated-resistance-in-pepper
#1
Asztéria Almási, Katalin Nemes, Zsófia Csömör, István Tóbiás, László Palkovics, Katalin Salánki
The nonstructural protein (NSs) of Tomato spotted wilt virus (TSWV) was previously identified as an avirulence determinant for Tsw-based resistance on pepper. The NSs of wild-type (WT) and resistance-breaking (RB) TSWV strains isolated in Hungary had only two amino acid substitutions (104, 461). We have analysed the ability of the NSs and their point mutant variants to trigger Tsw-mediated hypersensitive responses and RNA silencing suppressor (RSS) activity in patch assays. We identified a single amino acid change at position 104 (T-A) that was responsible for the necrosis induction or loss, while a significant difference was not detected in the RSS activity of the two parental strains...
June 20, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28621378/the-hypothiocyanite-radical-oscn-and-its-isomers
#2
Zhuang Wu, Jian Xu, Qifan Liu, Xuelin Dong, Dingqing Li, Nicole Holzmann, Gernot Frenking, Tarek Trabelsi, Joseph S Francisco, Xiaoqing Zeng
A biologically relevant reactive sulfur species (RSS), the hypothiocyanite radical OSCN, is generated in the gas phase through flash vacuum pyrolysis (FVP) of trifluoromethyl sulfinyl cyanide CF3S(O)CN at ca. 1000 K. Upon UV light irradiation (365 nm), OSCN rearranges to novel isomers OSNC and SOCN, and further visible light irradiation (400 ± 20 nm) leads to reverse isomerization. The identification of OSCN, OSNC, and SOCN in cryogenic matrices (Ar and N2, 2.8 K) with IR spectroscopy is supported by quantum chemical calculations up to the CCSD(T)-F12/VTZ-F12 level...
June 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28612383/a-new-player-in-bacterial-sulfide-inducible-transcriptional-regulation
#3
David P Giedroc
Although hydrogen sulfide (H2 S) is perhaps best known as a toxic gas, the electron-rich H2 S functions as an energy source and electron donor for chemolithotrophic and photosynthetic bacteria, via sulfide oxidation, and is a universal substrate for cysteine biosynthesis. These distinct harmful and beneficial roles of H2 S suggest the need to "sense" prevailing concentrations of sulfide and downstream reactive sulfur species (RSS) and regulate the expression of genes mediating sulfide homeostasis. The paper by Li et al...
June 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28605412/accuracy-profiles-for-analyzing-residual-solvents-in-textiles-by-gc-ms
#4
Qibei Bao, Kejie Fu, Qingqing Ren, Yingying Zhong, Dan Qian
Excess residual solvents (RSs) in clothes or other textiles could be toxic and pose risks to both humans and the environment. N,N-Dimethylformamide (DMF), N,N-dimethylacetamide (DMAc) and 1-methyl-2-pyrrolidinone (NMP) are important chemicals frequently used as solvents in the textile industry. Several organizations have proposed limiting DMF, DMAc and NMP in textiles, but an appropriate detection method has not been proposed. A sensitive GC-MS method for the quantification of DMF, DMAc and NMP in textiles was developed...
June 9, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28598358/a-smartphone-indoor-localization-algorithm-based-on-wlan-location-fingerprinting-with-feature-extraction-and-clustering
#5
Junhai Luo, Liang Fu
With the development of communication technology, the demand for location-based services is growing rapidly. This paper presents an algorithm for indoor localization based on Received Signal Strength (RSS), which is collected from Access Points (APs). The proposed localization algorithm contains the offline information acquisition phase and online positioning phase. Firstly, the AP selection algorithm is reviewed and improved based on the stability of signals to remove useless AP; secondly, Kernel Principal Component Analysis (KPCA) is analyzed and used to remove the data redundancy and maintain useful characteristics for nonlinear feature extraction; thirdly, the Affinity Propagation Clustering (APC) algorithm utilizes RSS values to classify data samples and narrow the positioning range...
June 9, 2017: Sensors
https://www.readbyqxmd.com/read/28588521/the-effect-of-extreme-response-and-non-extreme-response-styles-on-testing-measurement-invariance
#6
Min Liu, Allen G Harbaugh, Jeffrey R Harring, Gregory R Hancock
Extreme and non-extreme response styles (RSs) are prevalent in survey research using Likert-type scales. Their effects on measurement invariance (MI) in the context of confirmatory factor analysis are systematically investigated here via a Monte Carlo simulation study. Using the parameter estimates obtained from analyzing a 2007 Trends in International Mathematics and Science Study data set, a population model was constructed. Original and contaminated data with one of two RSs were generated and analyzed via multi-group confirmatory factor analysis with different constraints of MI...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28582558/promed-mail-22-years-of-digital-surveillance-of-emerging-infectious-diseases
#7
Malwina Carrion, Lawrence C Madoff
ProMED-mail (ProMED) was launched in 1994 as an email service to identify unusual health events related to emerging and re-emerging infectious diseases and toxins affecting humans, animals and plants. It is used daily by public health leaders, government officials at all levels, physicians, veterinarians and other healthcare workers, researchers, private companies, journalists and the general public. Reports are produced and commentary provided by a global team of subject matter experts in a variety of fields including virology, parasitology, epidemiology, entomology, veterinary and plant disease specialists...
May 1, 2017: International Health
https://www.readbyqxmd.com/read/28569776/hiv-integrase-inhibitor-elvitegravir-impairs-rag-functions-and-inhibits-v-d-j-recombination
#8
Mayilaadumveettil Nishana, Namrata M Nilavar, Rupa Kumari, Monica Pandey, Sathees C Raghavan
Integrase inhibitors are a class of antiretroviral drugs used for the treatment of AIDS that target HIV integrase, an enzyme responsible for integration of viral cDNA into host genome. RAG1, a critical enzyme involved in V(D)J recombination exhibits structural similarity to HIV integrase. We find that two integrase inhibitors, Raltegravir and Elvitegravir, interfered with the physiological functions of RAGs such as binding, cleavage and hairpin formation at the recombination signal sequence (RSS), though the effect of Raltegravir was limited...
June 1, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28556823/belief-interval-of-dempster-shafer-theory-for-line-of-sight-identification-in-indoor-positioning-applications
#9
Jinwu Wu, Tingyu Zhao, Shang Li, Chung-Ming Own
Location data are among the most widely used contextual data in context-aware and ubiquitous computing applications. Numerous systems with distinct deployment costs and levels of positioning accuracy have been developed over the past decade for indoor positioning purposes. The most useful method focuses on the received signal strength (RSS) and provides a set of signal transmission access points. Furthermore, most positioning systems are based on non-line-of-sight (NLOS) rather than line-of-sight (LOS) conditions, and this cause ranging errors for location predictions...
May 30, 2017: Sensors
https://www.readbyqxmd.com/read/28556788/an-improved-compressive-sensing-and-received-signal-strength-based-target-localization-algorithm-with-unknown-target-population-for-wireless-local-area-networks
#10
Jun Yan, Kegen Yu, Ruizhi Chen, Liang Chen
In this paper a two-phase compressive sensing (CS) and received signal strength (RSS)-based target localization approach is proposed to improve position accuracy by dealing with the unknown target population and the effect of grid dimensions on position error. In the coarse localization phase, by formulating target localization as a sparse signal recovery problem, grids with recovery vector components greater than a threshold are chosen as the candidate target grids. In the fine localization phase, by partitioning each candidate grid, the target position in a grid is iteratively refined by using the minimum residual error rule and the least-squares technique...
May 30, 2017: Sensors
https://www.readbyqxmd.com/read/28545257/the-reactive-sulfur-species-concept-15-years-on
#11
REVIEW
Gregory I Giles, Muhammad Jawad Nasim, Wesam Ali, Claus Jacob
Fifteen years ago, in 2001, the concept of "Reactive Sulfur Species" or RSS was advocated as a working hypothesis. Since then various organic as well as inorganic RSS have attracted considerable interest and stimulated many new and often unexpected avenues in research and product development. During this time, it has become apparent that molecules with sulfur-containing functional groups are not just the passive "victims" of oxidative stress or simple conveyors of signals in cells, but can also be stressors in their own right, with pivotal roles in cellular function and homeostasis...
May 23, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28539199/respiratory-severity-score-and-extubation-readiness-in-very-low-birth-weight-infants
#12
Maroun J Mhanna, Narayan P Iyer, Scott Piraino, Mohit Jain
BACKGROUND: The respiratory severity score (RSS) is a byproduct of mean airway pressure (MAP) and fraction of inspired oxygen (FiO2). We sought to determine whether RSS could be used as a screening tool to predict extubation readiness in very low birth weight (VLBW) infants. METHODS: In a retrospective cohort study, medical records of all VLBW infants admitted to our unit (6/1/09-2/28/12) were reviewed for infants' demographics, prenatal characteristics, and medication use...
May 10, 2017: Pediatrics and Neonatology
https://www.readbyqxmd.com/read/28532984/prognostic-impact-of-residual-syntax-score-in-patients-with-st-elevation-myocardial-infarction-and-multivessel-disease-analysis-of-an-8-year-all-comers-registry
#13
Carlos Galvão Braga, Ana Belén Cid-Alvarez, Alfredo Redondo Diéguez, Belén Alvarez Alvarez, Diego López Otero, Raymundo Ocaranza Sánchez, Xoan Sanmartin Pena, Violeta González Salvado, Ramiro Trillo-Nouche, José R González-Juanatey
BACKGROUND: The residual SYNTAX score (rSS) was designed and validated to quantify the burden of residual coronary artery disease after percutaneous coronary intervention (PCI). The aim of this study was to assess the prognostic impact of rSS in patients with ST-segment elevation myocardial infarction (STEMI) and multivessel disease undergoing primary-PCI. METHODS: This retrospective cohort study included 1499 consecutive patients with STEMI who underwent primary-PCI between January 2008 and December 2015...
April 20, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28529445/regression-models-on-riemannian-symmetric-spaces
#14
Emil Cornea, Hongtu Zhu, Peter Kim, Joseph G Ibrahim
The aim of this paper is to develop a general regression framework for the analysis of manifold-valued response in a Riemannian symmetric space (RSS) and its association with multiple covariates of interest, such as age or gender, in Euclidean space. Such RSS-valued data arises frequently in medical imaging, surface modeling, and computer vision, among many others. We develop an intrinsic regression model solely based on an intrinsic conditional moment assumption, avoiding specifying any parametric distribution in RSS...
March 2017: Journal of the Royal Statistical Society. Series B, Statistical Methodology
https://www.readbyqxmd.com/read/28506936/assessment-of-long-term-mortality-in-patients-with-complex-coronary-artery-disease-undergoing-percutaneous-intervention-comparison-of-multiple-anatomical-and-clinical-prognostic-risk-scores
#15
Bruno L Janella, Carlos M Campos, Adriano Caixeta, Breno O Almeida, Fabio Sandoli Brito, Alexandre Abizaid, Marco A Perin
AIMS: Three-vessel and left main coronary artery disease (CAD) have important prognostic implications. Consequently, numerous risk scores have been developed to stratify patients with complex CAD. The aim of the present study is to compare the predictive performance of six risk scores for occurrence of 4-year all-cause mortality. METHODS AND RESULTS: From March 2008 to December 2012, 348 consecutive patients with complex CAD undergoing percutaneous coronary intervention in a tertiary center in São Paulo, Brazil were analyzed...
May 16, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28505876/intermittency-induced-criticality-in-a-resistor-inductor-diode-circuit
#16
Stelios M Potirakis, Yiannis Contoyiannis, Fotios K Diakonos, Michael P Hanias
The current fluctuations of a driven resistor-inductor-diode circuit are investigated here looking for signatures of critical behavior monitored by the driving frequency. The experimentally obtained time series of the voltage drop across the resistor (as directly proportional to the current flowing through the circuit) were analyzed by means of the method of critical fluctuations in analogy to thermal critical systems. Intermittent criticality was revealed for a critical frequency band signifying the transition between the normal rectifier phase in the low frequencies and a full-wave conducting, capacitorlike phase in the high frequencies...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28502113/epigenetic-modifications-of-the-vh-region-after-djh-recombination-in-pro-b-cells
#17
Yanying Dong, Caijun Wu, Xiaohui Zhao, Ping Zhang, Hua Zhang, Mingzhe Zheng, Shichang Li, Junna Jiao, Xiaozhuo Yu, Zhuangwei Lv, Yanhong Ji
The variable region of murine immunoglobulin heavy chain (Igh) is assembled by sequential DH -JH and VH -DJH recombination. The accessibility of Igh locus determines the order of rearrangement. Because of the large number of VH genes and lack of a suitable model, the epigenetic modifications of VH genes after DJH recombination have not previously been characterized. Here, we employed two v-Abl pro-B cell lines, in which Igh locus is in germline and DJH -recombined configuration, respectively. The DJH junction displays the characteristics of recombination center, such as high levels of activation-associated histone modifications and recombination activating gene protein (RAG) binding in DJH -rearranged pro-B cells, which extend the recombination center model proposed for germline Igh locus...
May 14, 2017: Immunology
https://www.readbyqxmd.com/read/28493977/robust-multiple-frequency-multiple-power-localization-schemes-in-the-presence-of-multiple-jamming-attacks
#18
Ahmed Abdulqader Hussein, Chee Yen Leow, Tharek A Rahman
Localization of the wireless sensor network is a vital area acquiring an impressive research concern and called upon to expand more with the rising of its applications. As localization is gaining prominence in wireless sensor network, it is vulnerable to jamming attacks. Jamming attacks disrupt communication opportunity among the sender and receiver and deeply impact the localization process, leading to a huge error of the estimated sensor node position. Therefore, detection and elimination of jamming influence are absolutely indispensable...
2017: PloS One
https://www.readbyqxmd.com/read/28489057/an-optimized-facile-procedure-to-synthesize-and-purify-allicin
#19
Frank Albrecht, Roman Leontiev, Claus Jacob, Alan J Slusarenko
Allicin is a reactive sulfur species (RSS) and defence substance from garlic (Allium sativum L.). The compound is a broad-spectrum antibiotic that is also effective against multiple drug resistant (MDR) strains. A detailed protocol for allicin synthesis based on diallyl-disulfide (DADS) oxidation by H₂O₂ using acetic acid as a catalyst was published in 2001 by Lawson and Wang. Here we report on improvements to this basic method, clarify the mechanism of the reaction and show that it is zero-order with respect to DADS and first-order with respect to the concentration of H₂O₂...
May 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28472181/hypertrophic-scar-regression-is-linked-to-the-occurrence-of-endothelial-dysfunction
#20
Xi-Qiao Wang, Fei Song, Ying-Kai Liu
Most microvessels have been shown to become stenosed or completely occluded during hypertrophic scar progression. Here, we examined the morphology of capillary endothelial cells (ECs) and fibroblasts using immunofluorescence staining for CD31 and alpha-smooth muscle actin (α-SMA) and electron microscopy. In addition, ECs and fibroblasts were isolated from scar tissues, and the levels of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor (PDGF), endothelin 1 (ET-1), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were assayed using ELISAs...
2017: PloS One
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