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https://www.readbyqxmd.com/read/29220697/metabolism-of-hydrogen-sulfide-h2s-and-production-of-reactive-sulfur-species-rss-by-superoxide-dismutase
#1
Kenneth R Olson, Yan Gao, Faihaan Arif, Kanika Arora, Shivali Patel, Eric R DeLeon, Thomas R Sutton, Martin Feelisch, Miriam M Cortese-Krott, Karl D Straub
Reactive sulfur species (RSS) such as H2S, HS•, H2Sn, (n = 2-7) and HS2•- are chemically similar to H2O and the reactive oxygen species (ROS) HO•, H2O2, O2•- and act on common biological effectors. RSS were present in evolution long before ROS, and because both are metabolized by catalase it has been suggested that "antioxidant" enzymes originally evolved to regulate RSS and may continue to do so today. Here we examined RSS metabolism by Cu/Zn superoxide dismutase (SOD) using amperometric electrodes for dissolved H2S, a polysulfide-specific fluorescent probe (SSP4), and mass spectrometry to identify specific polysulfides (H2S2-H2S5)...
November 20, 2017: Redox Biology
https://www.readbyqxmd.com/read/29206188/lightweight-biometric-sensing-for-walker-classification-using-narrowband-rf-links
#2
Tong Liu, Zhuo-Qian Liang
This article proposes a lightweight biometric sensing system using ubiquitous narrowband radio frequency (RF) links for path-dependent walker classification. The fluctuated received signal strength (RSS) sequence generated by human motion is used for feature representation. To capture the most discriminative characteristics of individuals, a three-layer RF sensing network is organized for building multiple sampling links at the most common heights of upper limbs, thighs, and lower legs. The optimal parameters of sensing configuration, such as the height of link location and number of fused links, are investigated to improve sensory data distinctions among subjects, and the experimental results suggest that the synergistic sensing by using multiple links can contribute a better performance...
December 5, 2017: Sensors
https://www.readbyqxmd.com/read/29204143/local-chromatin-features-including-pu-1-and-ikaros-binding-and-h3k4-methylation-shape-the-repertoire-of-immunoglobulin-kappa-genes-chosen-for-v-d-j-recombination
#3
Louise S Matheson, Daniel J Bolland, Peter Chovanec, Felix Krueger, Simon Andrews, Hashem Koohy, Anne E Corcoran
V(D)J recombination is essential for the generation of diverse antigen receptor (AgR) repertoires. In B cells, immunoglobulin kappa (Igκ) light chain recombination follows immunoglobulin heavy chain (Igh) recombination. We recently developed the DNA-based VDJ-seq assay for the unbiased quantitation of Igh VH and DH repertoires. Integration of VDJ-seq data with genome-wide datasets revealed that two chromatin states at the recombination signal sequence (RSS) of VH genes are highly predictive of recombination in mouse pro-B cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29194185/does-maturity-status-affect-the-relationship-between-anaerobic-speed-reserve-and-multiple-sprints-sets-performance-in-young-soccer-players
#4
Mohamed Amin Selmi, Badriya Al-Haddabi, Mohamed Haj Yahmed, Radhouane Haj Sassi
The purpose of this study is to examine the relationship between repeated-sprint sets (RSS) performance indices and anaerobic speed reserve (ASR) in young soccer players of different maturity status. One hundred and seventy nine young male soccer players (11.1 to 17.8 years) classified as pre- (n=50), circum- (n=60), or post- (n=69) peak height velocity (PHV) performed multi-stage shuttle run test (MRST) to measure maximal aerobic speed (MAS), 30-m sprint with 10-m splits to estimate maximal anaerobic speed (MAnS), and RSS test...
November 29, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29181778/retzius-space-reconstruction-following-transperitoneal-laparoscopic-robot-assisted-radical-prostatectomy-does-it-have-any-added-value
#5
Yasmin Abu-Ghanem, Zohar Dotan, Jacob Ramon, Dorit E Zilberman
Retzius space sparing (RSS) during laparoscopic robot-assisted radical prostatectomy (RALP) has been offered as an approach that reduces perioperative complications and enables faster gaining of full urinary continence due to bladder anatomy preservation. Retro and transperitoneal techniques have been proposed, whereby RSS has been implemented. We sought to explore whether Retzius space reconstruction (RSR) following transperitoneal RALP will be an advantageous step as well. A prospective registry database of 102 consecutive transperitoneal RALP cases performed by a single surgeon was reviewed...
November 27, 2017: Journal of Robotic Surgery
https://www.readbyqxmd.com/read/29176980/iragu-a-novel-inducible-and-reversible-mouse-model-for-ubiquitous-recombinase-activity
#6
Marie Bonnet, Leonor Morais Sarmento, Ana C Martins, Daniel Sobral, Joana Silva, Jocelyne Demengeot
Developing lymphocytes express the recombination activating genes (RAGs) 1 and 2 products that form a site specific recombinase complex (RAG), introducing double strand DNA breaks (DSBs) at recombination signal sequences (RSSs) flanking the V, D, and J gene segments in the antigen receptor loci. The subsequent steps in the reaction consist in the ligation of DSBs by ubiquitous enzymes of the non-homologous end joining DNA repair pathway. This mutagenesis process is responsible for the generation of the very large clonal diversity of T and B lymphocytes, itself allowing the recognition of a virtually open-ended antigenic universe...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29160797/target-tracking-with-sensor-navigation-using-coupled-rss-and-aoa-measurements
#7
Slavisa Tomic, Marko Beko, Rui Dinis, João Pedro Gomes
This work addresses the problem of tracking a signal-emitting mobile target in wireless sensor networks (WSNs) with navigated mobile sensors. The sensors are properly equipped to acquire received signal strength (RSS) and angle of arrival (AoA) measurements from the received signal, while the target transmit power is assumed not known. We start by showing how to linearize the highly non-linear measurement model. Then, by employing a Bayesian approach, we combine the linearized observation model with prior knowledge extracted from the state transition model...
November 21, 2017: Sensors
https://www.readbyqxmd.com/read/29159061/eeg-coherence-related-to-fmri-resting-state-synchrony-in-long-term-abstinent-alcoholics
#8
Valerie A Cardenas, Mathew Price, George Fein
Recent work suggests that faulty co-activation or synchrony of multiple brain regions comprising "networks," or an imbalance between opposing brain networks, is important in alcoholism. Previous studies showed higher fMRI resting state synchrony (RSS) within the executive control (inhibitory control and emotion regulation) networks and lower RSS within the appetitive drive network in long-term (multi-year) abstinent alcoholics (LTAA) vs. non substance abusing controls (NSAC). Our goal was to identify EEG networks that are correlated with the appetitive drive and executive function networks identified with fMRI in our previous alcohol studies...
2018: NeuroImage: Clinical
https://www.readbyqxmd.com/read/29156565/survey-on-the-performance-of-source-localization-algorithms
#9
José Manuel Fresno, Guillermo Robles, Juan Manuel Martínez-Tarifa, Brian G Stewart
The localization of emitters using an array of sensors or antennas is a prevalent issue approached in several applications. There exist different techniques for source localization, which can be classified into multilateration, received signal strength (RSS) and proximity methods. The performance of multilateration techniques relies on measured time variables: the time of flight (ToF) of the emission from the emitter to the sensor, the time differences of arrival (TDoA) of the emission between sensors and the pseudo-time of flight (pToF) of the emission to the sensors...
November 18, 2017: Sensors
https://www.readbyqxmd.com/read/29131804/assessing-the-performance-of-the-precise-dapt-and-paris-risk-scores-for-predicting-1-year-out-of-hospital-bleeding-in-acute-coronary-syndrome-patients
#10
Emad Abu-Assi, Sergio Raposeiras-Roubin, Rafael Cobas-Paz, Berenice Caneiro-Queija, Cristina Martínez-Reglero, José Manuel Rodríguez-Rodríguez, Antonio Baz, Andrés Íñiguez-Romo
AIMS: PRECISE-DAPT and PARIS risk scores (RSs) were recently developed to bleeding risk assessment in percutaneous coronary intervention (PCI) patients treated with dual antiplatelet therapy (DAPT). We aimed to assess the performance of these RSs at predicting out-of-hospital bleeding in patients with acute coronary syndrome (ACS). METHODS AND RESULTS:  Retrospectively, we studied 1926 consecutive ACS patients treated with PCI and DAPT. The performance of RSs for predicting one-year BARC type 2, 3 or 5 bleeding and BARC type 3 or 5 bleeding was assessed and compared...
November 14, 2017: EuroIntervention
https://www.readbyqxmd.com/read/29102273/hemodynamic-assessment-of-extra-cardiac-tricuspid-valves-using-particle-image-velocimetry
#11
Munirah Ismail, Foad Kabinejadian, Yen Ngoc Nguyen, Edgar Tay Lik Wui, Sangho Kim, Hwa Liang Leo
There has not been much progress in the development of transcatheter tricuspid valves to treat tricuspid regurgitation because of the difficulty in anchoring a stented valve onto the complex tricuspid annulus. Hence, the concept of heterotopic implantation of the transcatheter tricuspid valve onto the cavo-atrial junction was proposed. However, to date there has been no detailed in vitro investigation of the hemodynamic performance of this new device. The study utilises both 2-D and 3-D particle image velocimetry (PIV) to interrogate the flow patterns in the vicinity of the extra-cardiac tricuspid valves in an in vitro physiological flow loop, specifically at four measurement locations in the cavo-atrial anatomy...
December 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/29091197/rna-and-dna-sanger-sequencing-versus-next-generation-sequencing-for-hiv-1-drug-resistance-testing-in-treatment-naive-patients
#12
E K Alidjinou, J Deldalle, C Hallaert, O Robineau, F Ajana, P Choisy, D Hober, L Bocket
Background: Sanger sequencing of plasma RNA is the standard method for HIV-1 drug resistance testing in treatment-naive patients, but is limited by the non-detection of resistance-associated mutations (RAMs) with prevalence below approximately 20%. Objectives: We compared RNA and DNA Sanger sequencing (RSS and DSS) with RNA next-generation sequencing (NGS) for RAM detection in HIV-1 reverse transcriptase (RT), protease (PR) and integrase (IN) genes. Methods: Sanger sequencing was performed on RNA and DNA, following the recommendations of the French Agency for AIDS Research (ANRS)...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29090655/potyviral-gene-silencing-suppressor-hcpro-interacts-with-salicylic-acid-sa-binding-protein-3-to-weaken-sa-mediated-defense-responses
#13
Sylvain Poque, Hui-Wen Wu, Chung-Hao Huang, Hao-Wen Cheng, Wen-Chi Hu, Jun-Yi Yang, David Wang, Shyi-Dong Yeh
The viral infection process is a battle between host defense response and pathogen antagonizing action. Several studies have established a tight link between the viral RNA silencing suppressor (RSS) and the repression of salicylic acid (SA)-mediated defense responses, nonetheless host factors directly linking an RSS and the SA pathway remains unidentified. From yeast two-hybrid analysis, we identified an interaction between the potyviral RSS helper-component proteinase (HCPro) and SA-binding protein SABP3. Co-localization and bimolecular fluorescence complementation analyses validated the direct in vivo interaction between Turnip mosaic virus (TuMV) HCPro and the Arabidopsis homologue of SABP3, AtCA1...
November 1, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29079250/an-assessment-of-the-quality-of-ct-radiation-dose-information-on-the-internet
#14
Siobhán O'Neill, David Glynn, Kevin P Murphy, Karl James, Maria Twomey, Richard Kavanagh, Owen J O'Connor, Michael M Maher
OBJECTIVES: Our goal was to ascertain the quality of health information related to CT radiation exposure using a simulated Internet search and from incident information that may be available during day-to-day Internet usage. METHODS: We entered 14 terms relating to information on CT dose into the most commonly used Internet search engine and analyzed the first 100 web page results for each. A Rich Search Site (RSS) feed search was also used to evaluate incidental information over a 12-week period in 2014...
October 24, 2017: Journal of the American College of Radiology: JACR
https://www.readbyqxmd.com/read/29072677/the-impact-of-neurally-adjusted-ventilatory-assist-mode-on-respiratory-severity-score-and-energy-expenditure-in-infants-a-randomized-crossover-trial
#15
J L Rosterman, E K Pallotto, W E Truog, H Escobar, K A Meinert, A Holmes, H Dai, W M Manimtim
OBJECTIVE: Examine respiratory severity scores (RSS) (mean airway pressure × fraction of inspired oxygen) and resting energy expenditure (REE) on neurally adjusted ventilatory assist (NAVA) compared with synchronized intermittent mandatory ventilation with pressure controlled and supported breath (SIMV (PC)PS). STUDY DESIGN: A randomized, crossover trial in a level IV neonatal intensive care unit. Twenty-four patients were ventilated with NAVA or SIMV (PC) PS for 12 h and then crossed over to the alternative mode for 12 h...
October 26, 2017: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/29064408/avian-group-d-rotaviruses-structure-epidemiology-diagnosis-and-perspectives-on-future-research-challenges
#16
Pallavi Deol, Jobin Jose Kattoor, Shubhankar Sircar, Souvik Ghosh, Krisztián Bányai, Kuldeep Dhama, Yashpal Singh Malik
In 1981, a new virus (virus 132) was described for the first time with morphological and biochemical similarities to rotaviruses (RVs), but without antigenic similarity to any of the previously known rotavirus groups. Subsequently, it was re-designated as D/132, and formed a new serogroup among rotaviruses, the group D rotavirus (RVD). Since their identification, RVs are the leading cause of enteritis and diarrhea in humans and various animal species, and are also associated with abridged growth, particularly in avian species...
October 24, 2017: Pathogens
https://www.readbyqxmd.com/read/29056044/small-molecule-fluorescent-probes-for-imaging-and-detection-of-reactive-oxygen-nitrogen-and-sulfur-species-in-biological-systems
#17
Xiaoyun Jiao, Yong Li, Jinye Niu, Xilei Xie, Xu Wang, Bo Tang
No abstract text is available yet for this article.
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29052787/pentapeptide-insertion-scanning-mutational-analysis-of-turkey-herpesvirus-hvt063-reveals-residues-important-for-its-rna-silencing-suppression-activity
#18
Gang Jia, Wenjing Li, Meina Fan, Ru Yu, Yang Xu, Liwei Geng, Jiedao Zhang, Changxiang Zhu, Hongmei Liu
HVT063, an RNA-binding protein encoded by turkey herpesvirus, has been shown previously to suppress RNA silencing. Here, a scanning library produced by pentapeptide-insertion scanning mutagenesis was used to identify key residues associated with its RNA silencing suppressor (RSS) activity. Forty-two in-frame insertion mutants of HVT063 protein were evaluated for their RSS activity using the dual-luciferase transient expressing assay system. Sixteen mutations resulted in a loss of RSS activity, 20 mutations resulted in decreased RSS activity, and six mutations exhibited high RSS activity similar to wild-type HVT063...
October 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29040960/a-novel-s-sulfhydrated-human-serum-albumin-preparation-suppresses-melanin-synthesis
#19
Mayumi Ikeda, Yu Ishima, Ryo Kinoshita, Victor T G Chuang, Nanami Tasaka, Nana Matsuo, Hiroshi Watanabe, Taro Shimizu, Tatsuhiro Ishida, Masaki Otagiri, Toru Maruyama
Products of ultraviolet (UV) irradiation such as reactive oxygen species (ROS) and nitric oxide (NO) stimulate melanin synthesis. Reactive sulfur species (RSS) have been shown to have strong ROS and NO scavenging effects. However, the instability and low retention of RSS limit their use as inhibitors of melanin synthesis. The free thiol at Cys34 on human serum albumin (HSA) is highly stable, has a long retention and possess a high reactivity for RSS. We report herein on the development of an HSA based RSS delivery system...
October 11, 2017: Redox Biology
https://www.readbyqxmd.com/read/29033919/adaptation-of-methanogenic-inocula-to-anaerobic-digestion-of-maize-silage
#20
Martyna Wojcieszak, Adam Pyzik, Krzysztof Poszytek, Pawel S Krawczyk, Adam Sobczak, Leszek Lipinski, Otton Roubinek, Jacek Palige, Aleksandra Sklodowska, Lukasz Drewniak
A well-balanced microbial consortium is crucial for efficient biogas production. In turn, one of a major factor that influence on the structure of anaerobic digestion (AD) consortium is a source of microorganisms which are used as an inoculum. This study evaluated the influence of inoculum sources (with various origin) on adaptation of a biogas community and the efficiency of the biomethanization of maize silage. As initial inocula for AD of maize silage the samples from: (i) an agricultural biogas plant (ABP) which utilizes maize silage as a main substrate, (ii) cattle slurry (CS), which contain elevated levels of lignocelluloses materials, and (iii) raw sewage sludge (RSS) with low content of plant origin materials were used...
2017: Frontiers in Microbiology
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