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https://www.readbyqxmd.com/read/29458007/a-genomically-characterized-collection-of-high-grade-serous-ovarian-cancer-xenografts-for-preclinical-testing
#1
Paulina Cybulska, Jocelyn M Stewart, Azin Sayad, Carl Virtanen, Patricia A Shaw, Blaise Clarke, Natalie Stickle, Marcus Q Bernardini, Benjamin G Neel
High-grade serous ovarian cancer (HGSC) is the leading cause of morbidity and mortality from gynecologic malignancy. Overall survival remains low, due to the nearly ubiquitous emergence of platinum-resistance and the paucity of effective next-line treatments. Current cell culture-based models show limited similarity to HGSC and are therefore unreliable predictive models for pre-clinical evaluation of investigational drugs. This deficiency could help explain the low overall rate of successful drug development and the decades of largely unchanged approaches to HGSC treatment...
February 16, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29457460/stressful-surfaces-cell-metabolism-on-a-poorly-adhesive-substrate
#2
Richard L Surmaitis, Carlos J Arias, Joseph B Schlenoff
The adhesion and proliferation of cells are exquisitely sensitive to the nature of the surface to which they attach. Aside from cell counting, cell "health" on surfaces is typically established by measuring the metabolic rate with dyes that participate in the metabolic pathway or using "live/dead" assays with combinations of membrane permeable/impermeable dyes. The binary information gleaned from these tests-whether cells are attached or not, and whether they are living or dead-provides an incomplete picture of cell health...
February 19, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29457303/long-term-effects-of-art-what-do-animals-tell-us
#3
REVIEW
V Duranthon, P Chavatte-Palmer
Early stages of mammalian embryonic development are now known to be very sensitive to their microenvironment, with long term effects on fetal, postnatal and adult health, thus extending to these early stages the concept of Developmental Origin of Health and Disease (DoHaD). In this scientific context, and with 3% of births in developed countries, safety of Assisted Reproductive Techniques procedures becomes a matter of concern. Besides, embryo technologies in domestic mammals, using huge number of embryos, do not seem to evidence heavy impacts on adult phenotypes...
February 19, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29457285/vaginal-isolation-of-beta-haemolytic-streptococcus-from-bitches-with-and-without-neonatal-deaths-in-the-litters
#4
A E Guerrero, M C Stornelli, S B Jurado, G Giacoboni, G H Sguazza, R L de la Sota, M A Stornelli
The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2...
February 18, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29456735/metadherin-contributes-to-epithelial-mesenchymal-transition-and-paclitaxel-resistance-induced-by-acidic-extracellular-ph-in-nasopharyngeal-carcinoma
#5
Changyun Yu, Yong Liu, Zhaobing Qin
Paclitaxel resistance is a challenge to the treatment of nasopharyngeal carcinoma (NPC). An acidic extracellular pH (pHe ), a hallmark of solid tumors, is demonstrated to decrease the efficacy of chemotherapy. However, the precise function of acidic pHe in mediating chemotherapy in NPC remains unknown. In the present study, acidic pHe significantly decreased the cytotoxicity of paclitaxel in NPC cells. In addition, epithelial-mesenchymal transition (EMT)-like changes were observed in NPC cells cultured at acidic pHe ...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456226/three-cases-of-prune-belly-syndrome-at-the-lagos-state-university-teaching-hospital-ikeja
#6
Adaobi U Solarin, Elizabeth A Disu, Henry O Gbelee, Adeola B Animasahaun, Oluwatosin E Aremu, Eucharia Ogbuokiri, Gbemisola O Ogunnaike, Alaba Oladimeji
Prune belly syndrome (PBS) is a rare congenital disorder affecting 2.5 to 3.8/100,000 live births worldwide. Our objective of this report is to describe clinical manifestation, laboratory, and radiological characteristics of PBS in our patients, to highlight the limitations to offering appropriate patient care due to parents demanding discharge against medical advice and the need to increase the awareness regarding this rare disease. We report three cases; all referred after birth with lax abdominal wall, congenital anomalies of kidney, and urinary tract...
January 2018: Saudi Journal of Kidney Diseases and Transplantation
https://www.readbyqxmd.com/read/29456197/clinical-usefulness-and-accuracy-of-polymerase-chain-reaction-in-the-detection-of-bacterial-meningitis-agents-in-pediatric-cerebrospinal-fluid
#7
M Nour, A Alaidarous
Bacterial meningitis poses enormous healthcare challenges due to a high mortality, morbidity and sequelae. Neisseria (N.) meningitidis, Haemophilus (H.) influenzae, Streptococcus (S.) pneumoniae and S. agalactiae remain among the most prevalent infectious agents that cause bacterial meningitis in children. The objective of this study was the simultaneous detection of these pathogens in suspected cerebrospinal fluid (CSF) by using multiplex polymerase chain reaction (mPCR) and compare PCR results with standard diagnostics currently used in clinical practice...
February 15, 2018: Current Research in Translational Medicine
https://www.readbyqxmd.com/read/29456120/bladder-debris-on-ultrasound-as-a-predictor-for-positive-urine-culture-in-a-pediatric-population
#8
Scott M Stevenson, Glen A Lau, William C Andolsek, Angela P Presson, Patrick C Cartwright
INTRODUCTION: Debris within the bladder is commonly seen on ultrasound. The etiology of bladder debris is varied and the likelihood that urinary debris represents positive urine culture is under investigation. We hypothesize that bladder debris will increase the likelihood that a urine culture is positive compared to those without bladder debris. METHODS: We performed a retrospective review on children aged 0-17 years who had a voiding cystourethrogram with a catheterized urinalysis or urine culture, and who also had a renal and bladder ultrasound up to 1 week prior, the day of, or up to 2 days after their urine studies...
February 3, 2018: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/29455477/-application-value-of-xpert-mtb-rif-in-diagnosis-of-spinal-tuberculosis-and-detection-of-rifampin-resistance
#9
Yang-Hui Jin, Shi-Yuan Shi, Qi Zheng, Jian Shen, Xiao-Zhang Ying, Yi-Fan Wang
OBJECTIVE: To investigate the application value of Xpert MTB/RIF in diagnosis of spinal tuberculosis and detection of rifampin resistance. METHODS: The 109 pus specimens were obtained from patients who were primaryly diagnosed as spinal tuberculosis. All of the pus specimens were detected by acid-fast stain, liquid fast culturing by BACTEC MGIT 960 and Xpert MTB/RIF assay to definite the differences in sensitivity and specificity of mycobacterium tuberculosis among detecting methods...
September 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29455095/establishment-of-pancreatic-microenvironment-model-of-er-stress-quercetin-attenuates-%C3%AE-cell-apoptosis-by-invoking-nitric-oxide-cgmp-signaling-in-endothelial-cells
#10
Natarajan Suganya, Krishna Priya Mani, Dornadula Sireesh, Palanisamy Rajaguru, Mariappanadar Vairamani, Thiruppathi Suresh, Takayoshi Suzuki, Suvro Chatterjee, Kunka Mohanram Ramkumar
The involvement of endoplasmic reticulum (ER) stress in endothelial dysfunction and diabetes-associated complications has been well documented. Inhibition of ER stress represents a promising therapeutic strategy to attenuate endothelial dysfunction in diabetes. Recent attention has focused on the development of small molecule inhibitors of ER stress to maintain endothelial homeostasis in diabetes. Here we have developed a reliable, robust co-culture system that allows a study on the endothelial cells and pancreatic β-cells crosstalk under ER stress and validated using a known ER stress modulator, quercetin...
January 3, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29455093/quantitative-determination-of-a-potent-geranylgeranyl-diphosphate-synthase-inhibitor-using-lc-ms-ms-derivatization-and-application
#11
Yashpal S Chhonker, Staci L Haney, Robert A Matthiesen, David F Wiemer, Sarah A Holstein, Daryl J Murry
An isomeric mixture of homogeranyl/homoneryl triazole bisphosphonates (VSW1198) has previously been shown to be a potent inhibitor of geranylgeranyl diphosphate (GGDP) synthase (GGDPS) and of therapeutic interest for the treatment of multiple myeloma. We have developed and validated a selective and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantitation of both the E- and Z- isomers of VSW1198 in cell culture media, mouse plasma and tissues. VSW1198 and internal standard are extracted from the bio-matrices by solid-phase extraction, followed by derivatization using trimethylsilyldiazomethane...
February 6, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29454794/hcv-genotype-6a-escape-from-and-resistance-to-velpatasvir-pibrentasvir-and-sofosbuvir-in-robust-infectious-cell-culture-models
#12
Long V Pham, Santseharay Ramirez, Judith M Gottwein, Ulrik Fahnøe, Yi-Ping Li, Jannie Pedersen, Jens Bukh
BACKGROUND & AIMS: Chronic liver diseases caused by hepatitis C virus (HCV) genotype 6 are prevalent in Asia, and millions of people require treatment with direct-acting antiviral regimens, such as NS5A inhibitor velpatasvir combined with the NS5B polymerase inhibitor sofosbuvir. We developed infectious cell culture models of HCV genotype 6a infection to study the effects of these inhibitors and the development of resistance. METHODS: The consensus sequences of prototype strains HK2 (MG717925) and HK6a (MG717928), originating from serum of patients with chronic HCV infection, were determined by Sanger sequencing of genomes amplified by reverse transcription-PCR...
February 15, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29454739/spanish-and-catalan-translation-cultural-adaptation-and-validation-of-the-picker-patient-experience-questionnaire-15
#13
M J Bertran, M Viñarás, M Salamero, F Garcia, C Graham, A McCulloch, J Escarrabill
OBJECTIVE: To develop and test a culturally adapted core set of questions to measure patients' experience after in-patient care. MATERIAL AND METHODS: Following the methodology recommended by international guides, a basic set of patient experience questions, selected from Picker Institute Europe questionnaires (originally in English), was translated to Spanish and Catalan. Acceptability, construct validity and reliability of the adapted questionnaire were assessed via a cross-sectional validation study...
February 14, 2018: Revista de Calidad Asistencial: Organo de la Sociedad Española de Calidad Asistencial
https://www.readbyqxmd.com/read/29454653/diagnostic-yield-of-real-time-polymerase-chain-reaction-in-the-diagnosis-of-intrapartum-maternal-rectovaginal-colonization-by-group-b-streptococcus-a-systematic-review-with-meta-analysis
#14
Otto Henrique May Feuerschuette, Sheila Koettker Silveira, Ana Carolina Labor Cancelier, Rosemeri Maurici da Silva, Daisson José Trevisol, Jefferson Ricardo Pereira
Group B Streptococcus is the leading etiologic factor of neonatal sepsis. Intrapartum real-time polymerase chain reaction (RT-PCR) may allow faster and more accurate detection of maternal colonization. The aim of this study was to determine the sensitivity and specificity of RT-PCR when compared to the reference standard culture in selective broth media collected from rectovaginal tract from laboring women. We selected 15 studies that included 6368 women. Papers selection, data extraction, and quality assessment were carried out by two independent researchers...
February 2, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29454268/occurrence-and-distribution-of-antibiotics-in-mariculture-farms-estuaries-and-the-coast-of-the-beibu-gulf-china-bioconcentration-and-diet-safety-of-seafood
#15
Ruiling Zhang, Jiying Pei, Ruijie Zhang, Shaopeng Wang, Weibin Zeng, Dali Huang, Yi Wang, Yuanyuan Zhang, Yinghui Wang, Kefu Yu
The occurrence, distribution, bioconcentration and diet safety via seafood consumption of 19 antibiotics were investigated in eight closed mariculture ponds, four estuaries, two nearshore areas and one offshore area from the Beibu Gulf. Seventeen, 16, 15 and 7 antibiotics were detected at total concentrations of 43.2 - 885 ng L -1 , 22.4 - 118 ng L -1 , 22.7 - 24.5 ng L -1 , and 1.81-3.23 ng L -1 in the water of the above different areas, respectively. This indicates that the mariculture ponds are important sources of antibiotic pollution on the coast of the Beibu Gulf...
February 14, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29453793/cross-cultural-comparison-of-predictors-for-self-care-behaviors-in-patients-with-type-2-diabetes
#16
Kaori Ikeda, Shimpei Fujimoto, Beth Morling, Shiho Ayano-Takahara, Shin-Ichi Harashima, Yukiko Uchida, Nobuya Inagaki
The aim of this study is to evaluate how culture moderates the behavioral and psychosocial predictors of diabetes self-care activities. Patients with type 2 diabetes were recruited in the outpatient department at two sites: Kyoto University hospital in Japan and the Christiana Care Health System in the United States. The data was collected by survey using questionnaires including questions on the frequency of self-care activities, behavioral and psychosocial predictors, and other background information from 149 Japanese patients and 48 American patients...
February 17, 2018: Journal of Diabetes Investigation
https://www.readbyqxmd.com/read/29453257/a-facile-arsenazo-iii-based-assay-for-monitoring-rare-earth-element-depletion-from-cultivation-media-of-methanotrophic-and-methylotrophic-bacteria
#17
Carmen Hogendoorn, Paula Roszczenko-Jasińska, N Cecilia Martinez-Gomez, Johann de Graaff, Patrick Grassl, Arjan Pol, Huub J M Op den Camp, Lena J Daumann
Recently, methanotrophic and methylotrophic bacteria were found to utilize rare earth elements (REE). To monitor the REE-content in culture media of these bacteria we have developed a rapid screening method using the Arsenazo III (AS III) dye for spectrophotometric REE-detection in the low μM (0.1-10 μM) range. We designed this assay to follow La III and Eu III depletion from the culture medium by the acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV. The assay can also be modified to screen the uptake of other REE such as Pr III or to monitor the depletion of La III from growth media in neutrophilic methylotrophs such as Methylobacterium extorquens AM1...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29453194/comparison-of-generic-fluorescent-markers-for-detection-of-extracellular-vesicles-by-flow-cytometry
#18
Leonie de Rond, Edwin van der Pol, Chi M Hau, Zoltan Varga, Auguste Sturk, Ton G van Leeuwen, Rienk Nieuwland, Frank A W Coumans
BACKGROUND: Extracellular vesicles (EVs) in biofluids are potential biomarkers of disease. To explore the clinical relevance of EVs, a specific generic EV marker would be useful, one that does not require antibodies and binds to all EVs. Here we evaluated 5 commonly used generic markers for flow cytometry. METHODS: Flow cytometry (A60-Micro, Apogee) was used to evaluate the ability of the generic EV markers calcein acetoxymethyl ester, calcein acetoxymethyl ester violet, carboxyfluorescein succinimidyl ester (CFSE), 4-(2-[6-(dioctylamino)-2-naphthalenyl]ethenyl)-1-(3-sulfopropyl)pyridinium (di-8-ANEPPS), and lactadherin to stain EVs from MCF7 human breast adenocarcinoma cell line-conditioned culture medium [epithelial cell adhesion molecule positive (EpCAM + )] or platelet EVs from human plasma [integrin β3 positive (CD61 + )]...
February 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29452993/an-in-vitro-evaluation-of-the-efficacy-of-tedizolid-implications-for-the-treatment-of-skin-and-soft-tissue-infections
#19
Pierre Delpech, Muna ALeryan, Brian Jones, Curtis Gemmell, Sue Lang
Skin and soft tissue infections (SSTI) are among the most commonly occurring infections and evidence suggests that these are increasing world-wide. The aetiology is diverse, but Staphylococcus aureus predominate and these are often resistant to antimicrobials that were previously effective. Tedizolid is a new oxazolidinone-class antibacterial indicated for the treatment of adults with SSTI caused by Gram-positive pathogens, including S. aureus. The aim of this study was to evaluate the in vitro efficacy of tedizolid in comparison to other clinically used antibacterials against antibiotic sensitive- and resistant-staphylococci, grown in planktonic cultures and as biofilms reflecting the growth of the microorganism during episodes of SSTI...
January 10, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29451598/performance-of-cryptococcal-antigen-lateral-flow-assay-in-serum-cerebrospinal-fluid-whole-blood-and-urine-in-hiv-infected-patients-with-culture-proven-cryptococcal-meningitis-admitted-at-a-brazilian-referral-center
#20
José E Vidal, Carolina Toniolo, Adriana Paulino, Arnaldo L Colombo, Marilena Dos Anjos Martins, Cristina da Silva Meira, Renata Guise Soares Azevedo, Vera Lucia Pereira-Chioccola, Hélio Rodrigues Gomes, Marcia Dos Santos Lazera, Augusto C Penalva de Oliveira, David R Boulware
Cryptococcal meningitis is the most common cause of opportunistic meningitis in HIV-infected patients in Brazil and causes unacceptable high mortality rates. In this study, HIV-infected patients with a first episode of culture-proven cryptococcal meningitis in cerebrospinal fluid (CSF) were prospectively included in order to evaluate sensitivity of cryptococcal antigen (CrAg) lateral flow assay (LFA) in serum, CSF, whole blood (fingerstick), and fresh urine. In addition, HIV-infected patients with other neurological confirmed diseases were included in order to evaluate the specificity of CrAg LFA in serum...
2018: Revista do Instituto de Medicina Tropical de São Paulo
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