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https://www.readbyqxmd.com/read/29243936/detection-of-gallibacterium-anatis-by-taqman-fluorescent-quantitative-pcr
#1
Heping Huangfu, Wenbo Xu, Hongkui Wang, Qing Dong, Hongwei Guo, Yanting Sun, Yunxia Li, Wenwen Gao, Wuqing Wang, Jia Zhang, Jingke Shi, Haochun Pan, Congcong Li, Linkang Wang
To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis...
December 15, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29243404/molecular-viability-testing-of-viable-but-non-culturable-bacteria-induced-by-antibiotic-exposure
#2
Seunguk Lee, Sungwoo Bae
Nucleic acid amplification-based methods are limited by their inability to discriminate between viable and dead cells. To overcome this drawback, propidium monoazide (PMA) combined with qPCR has been used to differentiate viable from nonviable cells in environmental samples. However, assessing bacterial physiology using PMA-qPCR remains a challenge due to its incapability of detecting metabolic activities, leading to overestimation of the viable bacteria population under an inactivation condition (e.g. antibiotic treatments)...
December 15, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29243167/psychological-distress-among-indigenous-australian-cancer-survivors
#3
Gail Garvey, J Cunningham, M Janda, V Yf He, P C Valery
PURPOSE: The purpose of this study is to identify the level of and factors associated with distress in 155 Indigenous Australian cancer survivors approximately 6 months post-diagnosis. METHODS: The distress thermometer (DT) was used to assess clinically significant distress (defined as having a DT score ≥ 4). Logistic regression was used to identify sociodemographic and clinical factors associated with clinically significant distress. RESULTS: The mean distress score was 2...
December 14, 2017: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/29243060/synovial-aspiration-and-serological-testing-in-two-stage-revision-arthroplasty-for-prosthetic-joint-infection-evaluation-before-reconstruction-with-a-mean-follow-up-of-twenty%C3%A2-seven%C3%A2-months
#4
Heinrich M L Mühlhofer, C Knebel, Florian Pohlig, Susanne Feihl, Norbert Harrasser, Johannes Schauwecker, Rüdiger von Eisenhart-Rothe
INTRODUCTION: The two-stage revision protocol is the gold standard for controlling and treating low-grade prosthetic joint infections of total hip and total knee arthroplasty. The antibiotic pause for diagnostic reasons before reconstruction (stage two) is discussed in relation to the persistence of the infection and the development of resistant bacterial strains. Serological markers and a synovial analysis are commonly used to exclude the persistence of infection. Therefore, we asked (1) is the serological testing of C-reactive protein and leucocytes a valuable tool to predict a persistence of infection? and (2) what is the role of synovial aspiration of Plymethylmethacrylat (PMMA) spacers in hip and knee joints? MATERIALS AND METHODS: One hundred twelve patients who were MSIS criteria-positive for a prosthetic joint infection were studied, including 45 total hip arthroplasties (THA) and 67 total knee artrhoplasties (TKA) patients...
December 14, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/29242676/program-active-cognitive-behavioral-therapy-to-treat-depression-in-adults-with-type-2-diabetes-in-rural-appalachia
#5
Mary de Groot, Todd Doyle, Jennifer Averyt
High rates of type 2 diabetes (T2DM) and depression exist in rural Appalachia with limited access to psychotherapeutic treatment. No manualized cognitive behavioral therapy (CBT) treatment materials exist that are culturally tailored for individuals in this region with T2DM. We describe the development of the Program ACTIVE CBT intervention for use with adults with T2DM and depression by mental health providers in rural Appalachia. Qualitative and quantitative methods were used to test the feasibility and acceptability of Program ACTIVE...
August 2017: Journal of Cognitive Psychotherapy
https://www.readbyqxmd.com/read/29242673/mexican-origin-adolescents-educational-expectation-trajectories-intersection-of-nativity-sex-and-socioeconomic-status
#6
Norma J Perez-Brena, Melissa Y Delgado, Sue A Rodríguez De Jesús, Kimberly A Updegraff, Adriana J Umaña-Taylor
Expectancy value theory and a cultural-ecological framework are integrated in this study to examine the trajectories of 246 Mexican-origin adolescents' (Mage = 12.52, SDage = 0.58; 51% girls, 62% U.S.-born) educational expectations across eight years. Findings from a multilevel growth model revealed that early adolescents expected to complete a post-bachelor's degree, but expectations declined in middle adolescence and improved in late adolescence. This pattern was more pronounced for immigrant, compared to U...
January 2017: Journal of Applied Developmental Psychology
https://www.readbyqxmd.com/read/29241966/streptococcus-anginosus-is-associated-with-postoperative-intraabdominal-collections-in-appendicitis
#7
Thejasvi Subramanian, Ellen Jerome, Ian Jones, Ingo Jester
AIM OF STUDY: Streptococcus anginosus group (SA) (formerly Streptococcus milleri) are pathogens recognised to have a high risk of postoperative collection in appendicitis, although little data exist specifically in children. We performed a retrospective review of all microbiological data from appendicectomies to assess whether there was an association in children. METHODS: A retrospective case note review of patients admitted to a paediatric tertiary centre coded for appendicitis from January 2015 to October 2016 was completed...
November 12, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29241222/flavopiridol-enhances-abt-199-sensitivity-in-unfavourable-risk-multiple-myeloma-cells-in-vitro-and-in-vivo
#8
Liang Zhou, Yu Zhang, Deepak Sampath, Joel Leverson, Yun Dai, Maciej Kmieciak, Matthew Nguyen, Robert Z Orlowski, Steven Grant
BACKGROUND: The BCL-2-specific BH3-mimetic ABT-199 (venetoclax) has been reported to be principally active against favourable-risk multiple myeloma (MM) cells, prompting efforts to extend its activity to include more resistant, higher-risk MM subsets. METHODS: Effects of the CDK9 inhibitor flavopiridol (FP; alvocidib) on responses to ABT-199 were examined in MM cells. Cell death and protein expression were evaluated by western blot and immunofluorescence. Xenograft models were used to study combination effects in vivo...
December 14, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29240994/using-mutations-for-pesticide-resistance-to-identify-the-cause-of-toxicity-in-environmental-samples
#9
Donald P Weston, Helen C Poynton, Kaley Major, Gary Wellborn, Michael J Lydy, Christoph Moschet, Richard Edward Connon
Traditional Toxicity Identification Evaluations (TIE) are applied to identify causal agents in complex environmental samples showing toxicity, and rely upon physical or chemical manipulation of samples. However, mutations conferring toxicant resistance provide the opportunity for a novel biologically-based TIE. Populations within the Hyalella azteca complex from pesticide-affected waterways were two and three orders of magnitude more resistant to the pyrethroid cyfluthrin and the organophosphate chlorpyrifos, respectively, than laboratory-cultured H...
December 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29240907/primary-care-for-refugees-and-newly-arrived-migrants-in-europe-a-qualitative-study-on-health-needs-barriers-and-wishes
#10
Tessa van Loenen, Maria van den Muijsenbergh, Marrigje Hofmeester, Christopher Dowrick, Nadja van Ginneken, Enkeleint Aggelos Mechili, Agapi Angelaki, Dean Ajdukovic, Helena Bakic, Danica Rotar Pavlic, Erika Zelko, Kathryn Hoffmann, Elena Jirovsky, Elisabeth Sophie Mayrhuber, Michel Dückers, Trudy Mooren, Juul Gouweloos-Trines, László Kolozsvári, Imre Rurik, Christos Lionis
Background: In order to provide effective primary care for refugees and to develop interventions tailored to them, we must know their needs. Little is known of the health needs and experiences of recently arrived refugees and other migrants throughout their journey through Europe. We aimed to gain insight into their health needs, barriers in access and wishes regarding primary health care. Methods: In the spring of 2016, we conducted a qualitative, comparative case study in seven EU countries in a centre of first arrival, two transit centres, two intermediate-stay centres and two longer-stay centres using a Participatory Learning and Action research methodology...
December 12, 2017: European Journal of Public Health
https://www.readbyqxmd.com/read/29240776/microbiological-profile-and-risk-factors-for-in-hospital-mortality-of-infective-endocarditis-in-tertiary-care-hospitals-of-south-vietnam
#11
Hoang M Tran, Vien T Truong, Tam M N Ngo, Quoc P V Bui, Hoang C Nguyen, Trung T Q Le, Wojciech Mazur, Eugene Chung, John M Cafardi, Khanh P N Pham, Hoang H N Duong, Thach Nguyen, Vu T Nguyen, Vinh N Pham
OBJECTIVES: We aimed to evaluate the microbiological characteristics and risk factors for mortality of infective endocarditis in two tertiary hospitals in Ho Chi Minh City, south Vietnam. MATERIALS AND METHODS: A retrospective study of 189 patients (120 men, 69 women; mean age 38 ± 18 years) with the diagnosis of probable or definite infective endocarditis (IE) according to the modified Duke Criteria admitted to The Heart Institute or Tam Duc Hospital between January 2005 and December 2014...
2017: PloS One
https://www.readbyqxmd.com/read/29240313/deconvolution-of-images-from-3d-printed-cells-in-layers-on-a-chip
#12
Sean Yu, Pranav Joshi, Yi Ju Park, Kyeong-Nam Yu, Moo-Yeal Lee
Layer-by-layer cell printing is useful in mimicking layered tissue structures inside the human body and has great potential for being a promising tool in the field of tissue engineering, regenerative medicine, and drug discovery. However, imaging human cells cultured in multiple hydrogel layers in 3D-printed tissue constructs is challenging as the cells are not in a single focal plane. Although confocal microscopy could be a potential solution for this issue, it compromises the throughput which is a key factor in rapidly screening drug efficacy and toxicity in pharmaceutical industries...
December 14, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29240275/metabolic-stress-induces-modifications-in-the-epigenetic-program-of-preimplantation-bovine-embryos
#13
R Tremblay, I Dufort, M A Sirard
The mammalian embryo is sensitive to and adapts to its metabolic environment. The mother's metabolic health and nutrient availability, for example, can modulate the oviductal fluid composition and thus embryo development. In this project, we induced energetic stress in bovine embryos during early culture to observe the epigenetic responses associated with metabolic stress, using a treatment paradigm known to decrease blastocyst rates. Embryos were generated using oocytes from slaughtered cows, and then exposed to an elevated glucose concentration (5 mM versus 0...
December 14, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29238639/the-use-of-vacuum-dressings-for-dead-space-management-in-deep-surgical-site-infections-allows-implant-and-bone-graft-retention
#14
James P Watt, Robert N Dunn
Study Design: Retrospective, descriptive study. Objectives: Managing early surgical site infection following elective lumbar spine surgery remains a challenge with controversy regarding retention of instrumentation and bone graft. Wound closure may also pose considerable challenges. We aim to report on our method of managing deep surgical site infections complicating elective spine surgery with surgeon assembled deep vacuum dressings. Identification of causative organisms with their sensitivities was a secondary objective...
December 2017: Global Spine Journal
https://www.readbyqxmd.com/read/29238587/bacterial-infections-in-acute-variceal-hemorrhage-despite-antibiotics-a-multicenter-study-of-predictors-and-clinical-impact
#15
Stephen Lee, Lynora Saxinger, Mang Ma, Verónica Prado, Joaquin Fernández, Deepali Kumar, Juan Gonzalez-Abraldes, Adam Keough, Ravin Bastiampillai, Michelle Carbonneau, Javier Fernandez, Puneeta Tandon
Background and aims: Current guidelines recommend antibiotic prophylaxis in all patients presenting with cirrhosis and acute variceal hemorrhage (AVH). We aimed to evaluate the characteristics and clinical impact of "early" infections (developing within 14 days) of AVH in a real-world setting. Methods: We analyzed retrospective data from a cohort of 371 adult patients with cirrhosis and AVH all of whom had received antibiotic prophylaxis (74% men; mean age 56 years), admitted to tertiary care hospitals in Edmonton, Alberta, Canada, and Barcelona, Spain...
December 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/29238331/sensitive-and-rapid-detection-of-the-plasmid-encoded-colistin-resistance-gene-mcr-1-in-enterobacteriaceae-isolates-by-loop-mediated-isothermal-amplification
#16
Dayang Zou, Simo Huang, Hong Lei, Zhan Yang, Yuxin Su, Xiaoming He, Qinghe Zhao, Yong Wang, Wei Liu, Liuyu Huang
The emergence of the plasmid-encoded colistin-resistance gene mcr-1 in Enterobacteriaceae represents a new threat to the treatment of infection in the clinical setting. A sensitive and rapid molecular method for detection of the mcr-1 gene in clinical isolates is needed to control the spread of this gene. In this study, we established a loop-mediated isothermal amplification (LAMP) assay for rapid detection of the mcr-1 gene. This assay was applied to cultured bacteria and spiked human stools. Real-time monitoring of turbidity and chromogenic visualization were used to assess the reaction results...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29237757/urine-lipoarabinomannan-glycan-in-hiv-negative-patients-with-pulmonary-tuberculosis-correlates-with-disease-severity
#17
Luisa Paris, Ruben Magni, Fatima Zaidi, Robyn Araujo, Neal Saini, Michael Harpole, Jorge Coronel, Daniela E Kirwan, Hannah Steinberg, Robert H Gilman, Emanuel F Petricoin, Roberto Nisini, Alessandra Luchini, Lance Liotta
An accurate urine test for pulmonary tuberculosis (TB), affecting 9.6 million patients worldwide, is critically needed for surveillance and treatment management. Past attempts failed to reliably detect the mycobacterial glycan antigen lipoarabinomannan (LAM), a marker of active TB, in HIV-negative, pulmonary TB-infected patients' urine (85% of 9.6 million patients). We apply a copper complex dye within a hydrogel nanocage that captures LAM with very high affinity, displacing interfering urine proteins. The technology was applied to study pretreatment urine from 48 Peruvian patients, all negative for HIV, with microbiologically confirmed active pulmonary TB...
December 13, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/29237446/trichomoniasis-and-associated-co-infections-of-the-genital-tract-among-pregnant-women-presenting-at-two-hospitals-in-ghana
#18
Richard H Asmah, Harriet N A Blankson, Kekeli A Seanefu, Noah Obeng-Nkrumah, Georgina Awuah-Mensah, Momodou Cham, Patrick F Ayeh-Kumi
BACKGROUND: Trichomonas vaginalis (TV) infection is the most prevalent non-viral sexually transmitted pathogen worldwide. Among pregnant women, the infection may cause adverse birth outcomes such as premature rupture of membranes and premature labour. In view of the paucity of information relating to TV among Ghanaian pregnant women, this study investigated its prevalence and associated co-infections among pregnant women. METHODS: High vaginal swabs were obtained from 99 pregnant women using sterile cotton swab sticks...
December 13, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/29237381/targeting-fgfr-with-bgj398-in-breast-cancer-effect-on-tumor-growth-and-metastasis
#19
Ana Sahores, Maria May, Gonzalo Sequeira, Cynthia Fuentes, Britta Jacobsen, Claudia Lanari, Caroline Ana Lamb
BACKGROUND: Endocrine resistance and metastatic dissemination comprise major clinical challenges for breast cancer treatment. The fibroblast growth factor receptor family (FGFR) consists of four tyrosine kinase transmembrane receptors, involved in key biological processes. Genomic alterations in FGFR have been identified in advanced breast cancer and thus, FGFR are an attractive therapeutic target. However, the efficacy of FGFR inhibitors on in vivo tumor growth is still controversial...
December 13, 2017: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/29237190/determination-of-acid-and-neutral-cannabinoids-in-extracts-of-different-strains-of-cannabis-sativa-using-gc-fid
#20
Elsayed A Ibrahim, Waseem Gul, Shahbaz W Gul, Brandon J Stamper, Ghada M Hadad, Randa A Abdel Salam, Amany K Ibrahim, Safwat A Ahmed, Suman Chandra, Hemant Lata, Mohamed M Radwan, Mahmoud A ElSohly
Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C...
December 13, 2017: Planta Medica
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