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Cultural sensitivity

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https://www.readbyqxmd.com/read/28647805/a-comprehensive-health-profile-of-guyanese-immigrants-aged-18-64-in-schenectady-new-york
#1
Akiko S Hosler, Jamie R Kammer
Guyanese immigrants are the 5th largest foreign-born group in NY State, but sparse literature is available on their health status and health needs. A community-based health interview survey of Schenectady NY residents aged 18-64 (N = 1861) was analyzed. Bivariate comparisons between Guyanese respondents and White, Black, and Hispanic respondents were made for each variable to identify disparities. As predominantly low SES immigrants, Guyanese adults were less likely to be covered by health insurance, have a usual place for care, and receive cancer screenings...
June 24, 2017: Journal of Immigrant and Minority Health
https://www.readbyqxmd.com/read/28647334/test-characteristics-of-milk-amyloid-a-elisa-somatic-cell-count-and-bacteriological-culture-for-detection-of-intramammary-pathogens-that-cause-subclinical-mastitis
#2
S Jaeger, F Virchow, P R Torgerson, M Bischoff, B Biner, S Hartnack, S R Rüegg
Bovine mastitis is an important disease in the dairy industry, causing economic losses as a result of withheld milk and treatment costs. Several studies have suggested milk amyloid A (MAA) as a promising biomarker in the diagnosis of mastitis. In the absence of a gold standard for diagnosis of subclinical mastitis, we estimated the diagnostic test accuracy of a commercial MAA-ELISA, somatic cell count (SCC), and bacteriological culture using Bayesian latent class modeling. We divided intramammary infections into 2 classes: those caused by major pathogens (e...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647256/bioavailability-of-wastewater-derived-dissolved-organic-nitrogen-to-green-microalgae-selenastrum-capricornutum-chlamydomonas-reinhardtii-and-chlorella-vulgaris-with-without-presence-of-bacteria
#3
Jingyi Sun, Halis Simsek
Effluent dissolved organic nitrogen (DON) is problematic in nutrient sensitive surface waters and needs to be reduced to meet demanding total dissolved nitrogen discharge limits. Bioavailable DON (ABDON) is a portion of DON utilized by algae or algae+bacteria, while biodegradable DON (BDON) is a portion of DON decomposable by bacteria. ABDON and BDON in a two-stage trickling filter (TF) wastewater treatment plant was evaluated using three different microalgal species, Selenastrum capricornutum, Chlamydomonas reinhardtii and Chlorella vulgaris and mixed cultured bacteria...
July 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28646949/recognition-and-diagnosis-of-invasive-fungal-infections-in-neonates
#4
Joanne L Calley, Adilia Warris
Fungal infections largely caused by Candida species are responsible for a significant disease burden in neonates and invasive candidiasis in hospitalised neonates has high associated morbidity and mortality. Early initiation of antifungal treatment improves outcome but the recognition and diagnosis of systemic fungal infection in this population is difficult due to the non-specific clinical presentation and the high false negative rate of cultures. There is a need for a practical, sensitive and rapid diagnostic test to enhance identification and early treatment...
June 2017: Journal of Infection
https://www.readbyqxmd.com/read/28646859/comparison-of-different-nat-assays-for-the-detection-of-microorganisms-belonging-to-the-class-mollicutes
#5
O Vega-Orellana, J B Poveda, R S Rosales, J M Bradbury, C G Poveda, L E Mederos-Iriarte, M M Tavío, A S Ramírez
BACKGROUND: Mollicutes detection can be cumbersome due to their slow growth in vitro. For this reason, the use of DNA based on generic molecular tests represents an alternative for rapid, sensitive and specific detection of these microorganism. For this reason, six previously described nucleic acid testing assays were compared to evaluate their ability to detect microorganisms belonging to the class Mollicutes. METHODS: A panel of 61 mollicutes, including representatives from the Mycoplasma, Acholeplasma, Mesoplasma, Spiroplasma and Ureaplasma genus, were selected to evaluate the sensitivity and specificity of these assays...
June 24, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28646666/detection-of-mycobacterium-avium-subspecies-paratuberculosis-in-powdered-infant-formula-using-is900-quantitative-pcr-and-liquid-culture-media
#6
Kamal R Acharya, Navneet K Dhand, Richard J Whittington, Karren M Plain
Mycobacterium avium subspecies paratuberculosis (MAP) has been implicated in Crohn's disease in humans resulting in public concern over the presence of MAP in powdered infant formula, which could contribute towards early human exposure to MAP or MAP components. Testing of representative powdered infant formula samples using effective tests is required to provide information on contamination of infant formula with MAP, so that consumers can make informed decisions. This study aimed to test representative powdered infant formula samples for the presence of MAP using a quantitative PCR and liquid culture method...
June 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28646354/associations-between-race-and-eating-disorder-symptom-trajectories-in-black-and-white-girls
#7
Lindsay P Bodell, Jennifer E Wildes, Yu Cheng, Andrea B Goldschmidt, Kate Keenan, Alison E Hipwell, Stephanie D Stepp
Epidemiological research suggests racial differences in the presentation of eating disorder symptoms. However, no studies have examined associations between race and eating disorder symptom trajectories across youth and adolescence, which is necessary to inform culturally sensitive prevention programs. The purpose of the current study was to examine the trajectories of eating disorder symptoms from childhood to young adulthood and to examine whether race was associated with trajectory group membership. Data were drawn from 2,305 Black and White girls who participated in a community-based longitudinal cohort study (Pittsburgh Girls Study) examining the development of psychopathology...
June 23, 2017: Journal of Abnormal Child Psychology
https://www.readbyqxmd.com/read/28645756/an-aptamer-based-pcr-method-coupled-with-magnetic-immunoseparation-for-sensitive-detection-of-salmonella-typhimurium-in-ground-turkey
#8
Lijun Wang, Ronghui Wang, Hong Wang, Michael Slavik, Hua Wei, Yanbin Li
Aptamers are single-stranded oligonucleotide ligands that can bind to targets with high affinity and specificity. They have been widely studied in the field of diagnostics as alternatives to antibodies due to their favorable features such as easy labeling, temperature tolerance, lower cost and recognition of a wide variety of targets. In this study, an aptamer-based PCR method coupled with magnetic immunoseparation was developed to detect S. Typhimurium from ground turkey. Firstly, biotinylated polyclonal anti-S...
June 20, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28645279/diabetes-care-in-a-complex-humanitarian-emergency-setting-a-qualitative-evaluation
#9
Adrianna Murphy, Michel Biringanine, Bayard Roberts, Beverley Stringer, Pablo Perel, Kiran Jobanputra
BACKGROUND: Evidence is urgently needed from complex emergency settings to support efforts to respond to the increasing burden of diabetes mellitus (DM). We conducted a qualitative study of a new model of DM health care (Integrated Diabetic Clinic within an Outpatient Department [IDC-OPD]) implemented by Médecins Sans Frontières (MSF) in Mweso Hospital in eastern Democratic Republic of Congo (DRC). We aimed to explore patient and provider perspectives on the model in order to identify factors that may support or impede it...
June 23, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28645157/secretory-iga-response-against-pseudomonas-aeruginosa-in-the-upper-airways-and-the-link-with-chronic-lung-infection-in-cystic-fibrosis
#10
Renan Mauch, Claudio Rossi, Talita Aiello, José Ribeiro, Antonio Ribeiro, Niels Høiby, Carlos Levy
We assessed the diagnostic ability of an ELISA test for measurement of specific secretory IgA (sIgA) in saliva to identify Cystic Fibrosis (CF) patients with P. aeruginosa chronic lung infection and intermittent lung colonization. A total of 102 Brazilian CF patients and 53 healthy controls were included. Specific serum IgG response was used as a surrogate to distinguish CF patients according to their P. aeruginosa colonization/infection status. The rate of sIgA positivity was 87.1% in CF chronically infected patients (Median value = 181...
June 22, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28645088/risk-based-screening-combined-with-a-pcr-based-test-for-group-b-streptococci-diminishes-the-use-of-antibiotics-in-laboring-women
#11
Mohammed R Khalil, Niels Uldbjerg, Poul B Thorsen, Birgitte Henriksen, Jens K Møller
OBJECTIVE: To assess the performance of a polymerase chain reaction - group B streptococci test (PCR-GBS test) - in deciding antibiotic prophylaxis in term laboring women. STUDY DESIGN: In this observational study, we enrolled 902 unselected Danish term pregnant women. During labor, midwives obtained vaginal swabs that were used for both GBS cultures (reference standard) and for the PCR-GBS test. Furthermore, we recorded the presence of risk factors for EOGBS (Early Onset Group B Streptococcal disease): (1) Bacteriuria during current pregnancy, (2) Prior infant with EOGBS (3) Temperature above 38...
June 9, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28644407/cotton-ascorbate-oxidase-promotes-cell-growth-in-cultured-tobacco-bright-yellow-2-cells-through-generation-of-apoplast-oxidation
#12
Rong Li, Shan Xin, Chengcheng Tao, Xiang Jin, Hongbin Li
Ascorbate oxidase (AO) plays an important role in cell growth through the modulation of reduction/oxidation (redox) control of the apoplast. Here, a cotton (Gossypium hirsutum) apoplastic ascorbate oxidase gene (GhAO1) was obtained from fast elongating fiber tissues. GhAO1 belongs to the multicopper oxidase (MCO) family and includes a signal peptide and several transmembrane regions. Analyses of quantitative real-time polymerase chain reaction (QRT-PCR) and enzyme activity showed that GhAO1 was expressed abundantly in 15-day post-anthesis (dpa) wild-type (WT) fibers in comparison with fuzzless-lintless (fl) mutant ovules...
June 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28643434/improved-yield-of-minimal-proportional-sample-volume-platelet-bacterial-culture
#13
Hany Kamel, Mary Townsend, Marjorie Bravo, Ralph R Vassallo
BACKGROUND: Reports of septic transfusion reactions (STRs) after transfusion of culture-negative platelets (PLTs) justify more effective prevention strategies. Pathogen reduction technologies or performance of additional point-of-issue testing are proposed strategies to enhance safety through Day 5 of storage. STUDY DESIGN AND METHODS: Trima leukoreduced apheresis PLTs (APs) were collected during two study periods (45 and 31 months) using standard procedures, with target settings adjusted during the second period to maintain split rate after increased culture volume...
June 22, 2017: Transfusion
https://www.readbyqxmd.com/read/28642144/performance-of-aspergilluspcr-in-cerebrospinal-fluid-for-the-diagnosis-of-cerebral-aspergillosis
#14
Sébastien Imbert, Jean-Yves Brossas, Martine Palous, Isabelle Joly, Isabelle Meyer, Arnaud Fekkar
OBJECTIVES: cerebral aspergillosis is a rare but often fatal form of invasive aspergillosis that remains difficult to diagnose. The literature has shown the value of Aspergillus PCR in blood-derived samples for the diagnosis of invasive aspergillosis but provides far less information for cerebrospinal fluid (CSF) in cerebral aspergillosis. Here, we evaluated the usefulness of an Aspergillus PCR assay performed on CSF for the diagnosis of cerebral aspergillosis. METHODS: this retrospective study involved 72 patients with suspected cerebral aspergillosis for a total of 88 CSF samples in whom CSF Aspergillus PCR was performed...
June 19, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28642141/added-diagnostic-value-and-impact-on-antimicrobial-therapy-of-16s-rrna-pcr-and-amplicon-sequencing-on-resected-heart-valves-in-infective-endocarditis-a-prospective-cohort-study
#15
Bart Peeters, Paul Herijgers, Kurt Beuselinck, Jan Verhaegen, Willy E Peetermans, Marie-Christine Herregods, Stefanie Desmet, Katrien Lagrou
OBJECTIVES: For adequate management and therapy of infective endocarditis (IE), identification of the causative pathogen is crucial but currently molecular testing results are not included in diagnostic criteria. The added diagnostic value and impact on antimicrobial therapy of 16S rRNA PCR and Amplicon Sequencing (16S rRNA PCR) performed on excised heart valves from IE patients was evaluated alongside with the effect of pre-operative antibiotics on the performance of blood culture (BC), valve culture (VC) and 16S rRNA PCR...
June 19, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28641870/antibiotic-activity-and-concentrations-in-clinical-samples-from-patients-with-chronic-bacterial-prostatitis
#16
V Heras-Cañas, B Gutiérrez-Soto, H Almonte-Fernández, A Lara-Oya, J M Navarro-Marí, A Garrido-Frenich, F Vázquez-Alonso, J Gutiérrez-Fernández
OBJECTIVES: Chronic bacterial prostatitis (CBP) is the most common urological disease in patients younger than 50 years, whose long-standing symptoms could be related to an inappropriate therapeutic regimen. The objective was to analyse the sensitivity of microorganisms isolated from patients with CBP and measure the weekly antibiotic concentrations in serum, semen and urine. MATERIAL AND METHODS: For the antibiotic sensitivity study, 60 clinical isolates were included between January 2013 and December 2014 from semen samples from patients with microbiologically confirmed CBP...
June 19, 2017: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/28641402/ec-tagging-allows-cell-type-specific-rna-analysis
#17
Naoki Hida, Mohamed Y Aboukilila, Dana A Burow, Rakesh Paul, Marc M Greenberg, Michael Fazio, Samantha Beasley, Robert C Spitale, Michael D Cleary
Purification of cell type-specific RNAs remains a significant challenge. One solution involves biosynthetic tagging of target RNAs. RNA tagging via incorporation of 4-thiouracil (TU) in cells expressing transgenic uracil phosphoribosyltransferase (UPRT), a method known as TU-tagging, has been used in multiple systems but can have limited specificity due to endogenous pathways of TU incorporation. Here, we describe an alternative method that requires the activity of two enzymes: cytosine deaminase (CD) and UPRT...
June 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28641046/exploring-the-health-needs-of-aging-lgbt-adults-in-the-cape-fear-region-of-north-carolina
#18
Noell L Rowan, Kelsey Beyer
This study explored issues of culturally sensitive health care practice and needs among lesbian, gay, bisexual and transgender aging adults in coastal North Carolina. Survey data from 2013 to 2015 were analyzed using frequencies and logistic regression on concerns with culturally sensitive health needs for the LGBT aging adult population. Results indicated that the largest problem was a history of being verbally harassed (n = 115; 51.57%) and a need for more culturally sensitive health care provisions. A large majority of participants requested an improvement in offering of LGBT specific social activities (n = 149), recreational activities (n = 116), and art and cultural activities (n = 112)...
June 22, 2017: Journal of Gerontological Social Work
https://www.readbyqxmd.com/read/28640908/evaluating-diagnostic-tests-for-bovine-tuberculosis-in-the-southern-part-of-germany-a-latent-class-analysis
#19
Valerie-Beau Pucken, Gabriela Knubben-Schweizer, Dörte Döpfer, Andreas Groll, Angela Hafner-Marx, Stefan Hörmansdorfer, Carola Sauter-Louis, Reinhard K Straubinger, Pia Zimmermann, Sonja Hartnack
Germany has been officially free of bovine tuberculosis since 1996. However, in the last years there has been an increase of bovine tuberculosis cases, particularly in the southern part of Germany, in the Allgäu region. As a consequence a one-time tuberculosis surveillance program was revisited with different premortal and postmortal tests. The aim of this paper was to estimate diagnostic sensitivities and specificities of the different tests used within this surveillance program. In the absence of a perfect test with 100% sensitivity and 100% specificity, thus in the absence of a gold standard, a Bayesian latent class approach with two different datasets was performed...
2017: PloS One
https://www.readbyqxmd.com/read/28640863/induced-pluripotent-stem-cell-derived-limbal-epithelial-cells-lipsc-as-a-cellular-alternative-for-in-vitro-ocular-toxicity-testing
#20
Edith Aberdam, Isabelle Petit, Linda Sangari, Daniel Aberdam
Induced pluripotent stem cells hold great potential to produce unlimited amount of differentiated cells as cellular source for regenerative medicine but also for in vitro drug screening and cytotoxicity tests. Ocular toxicity testing is mandatory to evaluate the risks of drugs and cosmetic products before their application to human patients by preventing eye irritation or insult. Since the global ban to use animals, many human-derived alternatives have been proposed, from ex-vivo enucleated postmortem cornea, primary corneal cell culture and immortalized corneal epithelial cell lines...
2017: PloS One
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