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https://www.readbyqxmd.com/read/28822610/evaluation-of-fever-in-the-emergency-department
#1
REVIEW
Sarah DeWitt, Summer A Chavez, Jack Perkins, Brit Long, Alex Koyfman
BACKGROUND: Fever is one of the most common complaints in the emergency department (ED) and is more complex than generally appreciated. The broad differential diagnosis of fever includes numerous infectious and non-infectious etiologies. An essential skill in emergency medicine is recognizing the pitfalls in fever evaluation. OBJECTIVE OF REVIEW: This review provides an overview of the complaint of fever in the ED to assist the emergency physician with a structured approach to evaluation...
August 14, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28822061/co-ultramicronized-palmitoylethanolamide-luteolin-facilitates-the-development-of-differentiating-and-undifferentiated-rat-oligodendrocyte-progenitor-cells
#2
Stephen D Skaper, Massimo Barbierato, Laura Facci, Mila Borri, Gabriella Contarini, Morena Zusso, Pietro Giusti
Oligodendrocytes, the myelin-producing cells of the central nervous system (CNS), have limited capability to bring about repair in chronic CNS neuroinflammatory demyelinating disorders such as multiple sclerosis (MS). MS lesions are characterized by a compromised pool of undifferentiated oligodendrocyte progenitor cells (OPCs) unable to mature into myelin-producing oligodendrocytes. An attractive strategy may be to replace lost OLs and/or promote their maturation. N-palmitoylethanolamine (PEA) is an endogenous fatty acid amide signaling molecule with anti-inflammatory and neuroprotective actions...
August 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28821529/antibiotic-susceptibility-profiles-of-ocular-and-nasal-flora-in-patients-undergoing-cataract-surgery-in-taiwan-an-observational-and-cross-sectional-study
#3
Yun-Hsuan Lin, Yu-Chuan Kang, Chiun-Ho Hou, Yhu-Chering Huang, Chih-Jung Chen, Jwu-Ching Shu, Pang-Hsin Hsieh, Ching-Hsi Hsiao
OBJECTIVE: To investigate the conjunctival and nasal flora and the antibiotic susceptibility profiles of isolates from patients undergoing cataract surgery. DESIGN: Observational and cross-sectional study. SETTING: A single-centre study in Taiwan. PARTICIPANTS: 128 consecutive patients precataract surgery. PRIMARY AND SECONDARY OUTCOME MEASURES METHODS: Conjunctival and nasal cultures were prospectively obtained from 128 patients on the day of cataract surgery before instillation of ophthalmic solutions in our hospital...
August 18, 2017: BMJ Open
https://www.readbyqxmd.com/read/28821461/cutaneous-vaccination-with-coated-microneedles-prevents-development-of-airway-allergy
#4
Akhilesh Kumar Shakya, Chang Hyun Lee, Harvinder Singh Gill
Allergy cases are increasing worldwide. Currently allergies are treated after their appearance in patients. However, now there is effort to make a preventive vaccine against allergies. The rationale is to target patient populations that are already sensitized to allergens but have yet to develop severe forms of the allergic disease, or who are susceptible to allergy development but have not yet developed them. Subcutaneous injections and the sublingual route have been used as the primary mode of preventive vaccine delivery...
August 15, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28821352/towards-optimisation-of-induced-pluripotent-cell-culture-extracellular-acidification-results-in-growth-arrest-of-ipsc-prior-to-nutrient-exhaustion
#5
Anja Wilmes, Caroline Rauch, Giada Carta, Georg Kern, Florian Meier, Wilfried Posch, Doris Wilflingseder, Lyle Armstrong, Majlinda Lako, Mario Beilmann, Gerhard Gstraunthaler, Paul Jennings
Human induced pluripotent stem cells (iPSC) have the potential to radically reduce the number of animals used in both toxicological science and disease elucidation. One initial obstacle culturing iPSC is that they require daily medium exchange. This study attempts to clarify why and propose some practical solutions. Two iPSC lineages were fed at different intervals in a full growth area (FGA) or a restricted growth area (RGA). The FGA consisted of a well coated with Matrigel™ and the RGA consisted of a coated coverslip placed in a well...
August 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28821251/evaluation-of-different-diagnostic-methods-for-the-detection-of-mycobacterium-avium-subsp-paratuberculosis-in-boot-swabs-and-liquid-manure-samples
#6
Nathalie Hahn, Klaus Failing, Tobias Eisenberg, Karen Schlez, Peter-Michael Zschöck, Karsten Donat, Esra Einax, Heike Köhler
BACKGROUND: Environmental sampling based on boot swabs and/or liquid manure samples is an upcoming strategy for the identification of paratuberculosis (paraTB) positive herds, but only limited data are available regarding the diagnostic performance of molecular detection methods (qPCR) versus faecal culture (FC) for this purpose. In the present study, the test characteristics of two different qPCR protocols (A and B) and a standardized FC protocol, for the detection of Mycobacterium avium subsp...
August 18, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28820784/impact-of-targeted-education-on-colorectal-cancer-screening-knowledge-and-psychosocial-attitudes-in-a-predominantly-hispanic-population
#7
Jennifer C Molokwu, Navkiran Shokar, Alok Dwivedi
Colorectal cancer remains a leading cause of cancer-related morbidity and mortality, with screening behavior found to be influenced by knowledge and other psychosocial attitudes. We recruited 784 participants 50 to 70 years of age. The intervention arm received a culturally sensitive, literacy-appropriate educational intervention by a promotora. Surveys were completed at baseline and 6 months post. Our intervention significantly increased knowledge at 6 months when compared with control (0.74 vs 0.18, P < ...
October 2017: Family & Community Health
https://www.readbyqxmd.com/read/28820621/-doctors-killed-my-baby-argumentative-patterns-in-medical-disputes-in-china
#8
Dawei Pan
The recent surge in medical disputes requires that more attention be paid to the voices of laypeople. This article explores the argumentative nature of the public discourse of the reported "malpractice mobs" that strive to convince members of the public (in the place of health or legal experts) that they have been victims of medical errors and injustice. This case-based qualitative empirical study uses a pragma-dialectical approach to suggest a prototypical pattern of such public appeals that contains a basic argumentative pattern and various types of extensions...
August 18, 2017: Health Communication
https://www.readbyqxmd.com/read/28820458/electrochemical-biosensors-for-rapid-detection-of-foodborne-salmonella-a-critical-overview
#9
REVIEW
Stefano Cinti, Giulia Volpe, Silvia Piermarini, Elisabetta Delibato, Giuseppe Palleschi
Abstract:Salmonella has represented the most common and primary cause of food poisoning in many countries for at least over 100 years. Its detection is still primarily based on traditional microbiological culture methods which are labor-intensive, extremely time consuming, and not suitable for testing a large number of samples. Accordingly, great efforts to develop rapid, sensitive and specific methods, easy to use, and suitable for multi-sample analysis, have been made and continue. Biosensor-based technology has all the potentialities to meet these requirements...
August 18, 2017: Sensors
https://www.readbyqxmd.com/read/28820395/chemokine-c-x-c-motif-ligand-1-cxcl1-and-chemokine-c-x-c-motif-ligand-2-cxcl2-modulate-the-activity-of-trpv1-ib4-cultured-rat-dorsal-root-ganglia-neurons-upon-short-term-and-acute-application
#10
A F Deftu, A Filippi, K Shibsaki, R O Gheorghe, M Chiritoiu, V Ristoiu
CXCL1 and CXCL2 are two chemokines with 78% homology of their sequence. CXCL1 was associated with atopic dermatitis, a highly pruritic skin disease, but it is not clear what is its mechanism of action, while for CXCL2 there are no data about an association with itch sensitivity. CXCL1 and CXCL2 can modulate TRPV1 receptors, which are one of the most important downstream effectors for itch sensitivity, upon short-term (4 h) or long-term (24 h) incubation, but the data are incomplete. Therefore, the aims of this study were to better characterize the short-term effects of CXCL1 and CXCL2 on TRPV1(+)/IB4(+) dorsal root ganglia neurons known to include nociceptor and itch-sensitive neurons, and to obtain new data about the acute application (12 min) of the two chemokines on the same population of neurons...
June 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28820354/validity-of-screening-for-psychiatric-disorders-in-unaccompanied-minor-asylum-seekers-use-of-computer-based-assessment
#11
Marianne Jakobsen, Melinda A Meyer DeMott, Trond Heir
Self-report screening is an important element of transcultural research. Problems concerning illiteracy, cultural sensitivity, and possible misunderstandings have been handled differently in different settings. The aim of this study was to evaluate the validity of two well-known instruments: the Hopkins Symptoms Check List (HSCL-25), and the Harvard Trauma Questionnaire (HTQ, Part IV), with a sample of 160 unaccompanied asylum-seeking adolescents from Afghanistan and Somalia. Assessments were performed 4 months after arrival in Norway, and the screening instruments were presented to the informants on computers with touch-screen function, using the program MultiCASI...
January 1, 2017: Transcultural Psychiatry
https://www.readbyqxmd.com/read/28819600/extensively-drug-resistant-tuberculosis-of-the-lumbar-spine-in-a-six-year-old-child-a-case-report
#12
Siddharth Sanjay Shah, Aakanksha Arvind Goregaonkar, Arvind Balkrishna Goregaonkar
INTRODUCTION: The emergence of extensively drug-resistant tuberculosis (XDR-TB) is a challenging paradigm shift faced by the TB control programs worldwide today. The treatment is further compounded with unique management difficulties faced in pediatric patients. Treatment of XDR-TB requires prolonged chemotherapy with second-line drugs which offer lesser potency and increased risk of drug-related side effects. We present a case of spinal XDR-TB in a child, managed with extended second-line antitubercular chemotherapy (ATT)...
March 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28819490/maternofetal-outcome-of-asymptomatic-bacteriuria-among-pregnant-women-in-a-nigerian-teaching-hospital
#13
Kenneth Ebele Izuchukwu, Emmanuel Okwudili Oranu, Goddy Bassey, Ngozi Clare Orazulike
INTRODUCTION: Asymptomatic bacteriuria has been reported to be associated with adverse pregnancy outcome. This study sought to determine the prevalence and complications of asymptomatic bacteriuria amongst parturient in the University of Port Harcourt Teaching Hospital (UPTH). METHODS: The study was a prospective cohort study involving 220 eligible antenatal attendees. Urine culture and sensitivity was conducted for each participant and the fetomaternal outcome between affected and unaffected women were compared and p value <0...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28818633/patient-and-caregiver-reported-assessment-tools-for-palliative-care-summary-of-the-2017-ahrq-technical-brief
#14
Rebecca Aslakson, Sydney M Dy, Renee F Wilson, Julie Waldfogel, Allen Zhang, Sarina R Isenberg, Alex Blair, Joshua Sixon, Karen A Robinson
CONTEXT: Assessment tools are data collection instruments that are completed by or with patients or caregivers and which collect data at the individual patient or caregiver level. OBJECTIVES: To (1) summarize palliative care assessment tools completed by or with patients or caregivers, and (2) identify needs for future tool development and evaluation. METHODS: We completed: (1) a systematic review of systematic reviews; (2) a supplemental search of previous reviews and websites, and/or (3) a targeted search for primary articles when no tools existed in a domain...
August 14, 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28817723/sequence-analysis-of-the-rifampicin-resistance-determining-region-rrdr-of-rpob-gene-in-multidrug-resistance-confirmed-and-newly-diagnosed-tuberculosis-patients-of-punjab-pakistan
#15
Salma Hameed, Kartyk Moganeradj, Nasir Mahmood, Timothy D McHugh, Muhammad Nawaz Chaudhry, Catherine Arnold
Molecular screening of new patients suspected for TB could help in the effective control of TB in Pakistan as it is a high TB burden country. It will be informative to understand the prevalence of multi drug resistance for a better drug regimen management in this geographical area. The Rifampicin resistance determining region (RRDR) sequencing was used to identify mutations associated with drug resistance in DNA extracts from 130 known multidrug resistant (MDR) cultured strains and compared with mutations observed in DNA extracts directly from 86 sputum samples from consecutive newly diagnosed cases in Lahore, Pakistan...
2017: PloS One
https://www.readbyqxmd.com/read/28816710/establishment-of-primary-cell-culture-from-ascitic-fluid-and-solid-tumor-obtained-from-epithelial-ovarian-carcinoma-patients
#16
Rajarshi Kar, Diwesh Chawla, Bindiya Gupta, Mohit Mehndiratta, Neelam Wadhwa, Rachna Agarwal
OBJECTIVE: Ovarian cancer is the seventh leading cause of cancer death worldwide. This is mainly due to late diagnosis and high rate of relapse and resistance following chemotherapy. In the present study, we describe simple and cost-effective method to establish primary culture from ascitic fluid and solid tumor obtained from epithelial ovarian carcinoma patient, which may provide a better tool for in vitro testing of drug sensitivity and designing individualized treatment protocol. METHODS: Complete Dulbecco modified Eagle medium (DMEM) was prepared by supplementing DMEM with 10% fetal bovine serum and antibiotics (ciprofloxacin and amphotericin B)...
August 16, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28816395/cultural-adaptation-and-validation-of-the-caring-behaviors-assessment-tool-in-chile
#17
Ricardo A Ayala, María Julia Calvo
This study reports the process of cultural adaptation of the Caring Behaviors Assessment tool for the Spanish language, and determine its validity and reliability. We used a mixed-methods approach with a sample of adult participants after translation and adjustment, correlations and multiple regressions were used to explain differences in perception. Internal consistency and reliability were determined by using Cronbach's alpha. While slight modifications to syntax, language, and the interval scale were necessary to enable better comprehension, all items had high average scores as did the seven subscales...
August 17, 2017: Nursing & Health Sciences
https://www.readbyqxmd.com/read/28815948/flow-cytometric-analysis-of-viable-bacteria-in-urine-samples-of-febrile-patients-at-the-emergency-department
#18
Anne H Tavenier, Foppie J de Boer, Bijan Moshaver, Sjef J C M van der Leur, Coen A Stegeman, Paul H P Groeneveld
BACKGROUND: Fast and reliable diagnostics are important in febrile patients admitted to the emergency department. Current urine diagnostics are fast but moderately reliable or reliable but time consuming. Flow cytometry (FC) is a new promising technique in the diagnostics of complicated urinary tract infections by counting bacteria in urine samples. The aim of this study is to improve the FC method by counting only viable bacteria. METHODS: Urine was obtained from 135 consecutive febrile patients at the emergency department...
August 16, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28815884/periodontal-ligament-like-tissue-regeneration-with-drilled-porous-decalcified-dentin%C3%A2-matrix-sheet-composite
#19
Ge Feng, Yan Wu, Yu Yong, Lan Huang, Shu An, Bo Hu, Jun Luo, Jinlin Song
OBJECTIVE: In this study, we constructed a composite by combining the human dental follicle cell sheet and a manual drilled porous decalcified dentin matrix that was used to construct ectopic tissue-engineered periodontal ligament-like tissues in renal capsules of nude mice. MATERIALS AND METHODS: Human dental follicle cells were harvested from human lower third molars and then embedded into a temperature-sensitive culture dish. These cells were then placed into frozen porous decalcified dentin matrix sheets and induced by 50 g/ml ascorbic acid...
August 16, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28814382/predictive-utility-of-swab-screening-for-vancomycin-resistant-enterococcus-in-selection-of-empiric-antibiotics-for-enterococcus-sterile-site-infections-a-retrospective-cohort-study
#20
Ian Brasg, Marion Elligsen, Derek MacFadden, Nick Daneman
BACKGROUND: Swab screening for vancomycin-resistant Enterococcus (VRE) is used to identify VRE carriers and thus prevent transmission of this organism. We tested whether screening results could have unintended benefits in predicting the vancomycin susceptibility of subsequent Enterococcus infections experienced by these patients. METHODS: We conducted a retrospective cohort study of all patients admitted to a large tertiary care hospital between 2010 and 2015 who underwent swab screening for VRE before experiencing an Enterococcus sterile-site infection...
August 15, 2017: CMAJ Open
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