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cancer and mycoplasma

Danyang Liu, Yang Hu, Ying Guo, Zhu Zhu, Bingzheng Lu, Xuelan Wang, Yijun Huang
Mycoplasma infection has been reported to be associated with cancer migration, invasion, epithelial-mesenchymal transition as well as the resistance to nucleoside analogues chemotherapeutic drugs. In this study, we found that the sensitivity of hepatocarcinoma cells to Cisplatin, Gemcitabine and Mitoxantrone was increased by mycoplasma elimination. Similar to the effect of anti-mycoplasma agent, interrupting the interaction between Mycoplasma hyorhinis membrane protein P37 and Annexin A2 of host cells using the N-terminal of ANXA2 polypeptide enhanced the sensitivity of HCC97L cells to Gemcitabine and Mitoxantrone...
2017: PloS One
Hassan Z A Ishag, Qiyan Xiong, Maojun Liu, Zhixin Feng, Guoqing Shao
Mycoplasma hyorhinis (M. hyorhinis) is an opportunistic pig pathogen, belonging to the class Mollicutes. It causes polyserositis, arthritis and cancers in vitro, increasing attention of the researchers. Currently, there is no available genetic tool to manipulate its genome. This study describes a development of oriC-plasmids harboring either large (pGEMT-LoriC) or minimum (pGEMT-MoriC) origin of replication (oriC) of M. hyorhinis along with tetracycline resistance marker.These plasmids were successfully transformed into M...
September 6, 2017: Scientific Reports
Shuyan Cao, Dandan Shen, Yadong Wang, Linxi Li, Liping Zhou, Yuxue Wang
Mycoplasma infection has been reported in immunocompromised cancer patients; nevertheless, it is not clear if persistent Mycoplasma infection could facilitate the proliferation of cancer cells in immunocompromised organisms. The aim of this study was to examine the relationship between persistent Mycoplasma infection and malignant transformation in an immunodeficient host model. Immunodeficient mouse model was established using cyclophosphamide and mice gastric mucosal cells were infected with Mycoplasma penetrans (Mpe)...
2017: PloS One
Zhi-Luo Deng, Szymon P Szafrański, Michael Jarek, Sabin Bhuju, Irene Wagner-Döbler
Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e...
June 16, 2017: Scientific Reports
Kasper Ingerslev, Estrid Hogdall, Tine Henrichsen Schnack, Wojciech Skovrider-Ruminski, Claus Hogdall, Jan Blaakaer
BACKGROUND: The etiological cause of ovarian cancer is poorly understood. It has been theorized that bacterial or viral infection as well as pelvic inflammatory disease could play a role in ovarian carcinogenesis. AIM: To review the literature on studies examining the association between ovarian cancer and bacterial or viral infection or pelvic inflammatory disease. METHODS: Database search through MEDLINE, applying the medical subject headings: "Ovarian neoplasms", AND "Chlamydia infections", "Neisseria gonorrhoeae", "Mycoplasma genitalium", "Papillomaviridae", or "pelvic inflammatory disease"...
2017: Infectious Agents and Cancer
Tatsuya Osaki, Tatsuto Kageyama, Yuka Shimazu, Dina Mysnikova, Shintaro Takahashi, Shinichi Takimoto, Junji Fukuda
Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplasma or other types of cells, have compromised the reproducibility and reliability of cell-based approaches. Here, we propose an epi relief-contrast cellular monitoring system (eRC-CMS) that allows images of cells in a typical culture plate to be acquired, stored, and analysed for daily cell quality control...
May 15, 2017: Scientific Reports
Yingying Zhu, Naouel Ailane, Monica Sala-Valdés, Farhad Haghighi-Rad, Martine Billard, Viet Nguyen, Raphael Saffroy, Antoinette Lemoine, Eric Rubinstein, Claude Boucheix, Céline Greco
Colorectal carcinoma cells Isreco1 display an ability to migrate controlled by a complex set of signals issued from the membrane. By comparing cells infected by mycoplasmas and mycoplasmas free cells, we have established that basal 2D migration is dependent on a double signal mediated by the collagen receptors integrins alpha1/2 and the Toll-Like receptor TLR2. The signal issued from mycoplasmas can be replaced by a TLR2 ligand and the functional effect is neutralized by silencing of MyD88. Following previous observation that downregulation of E-cadherin/p120 catenin increases cell motility, we now report that EGFR or CD44 inhibition have a similar effect on cell motility that is restricted to tetraspanin Co-029/tspan8 transduced IsrecoI cells (Is1-Co029)...
April 18, 2017: Oncotarget
Raina Fichorova, Jorge Fraga, Paola Rappelli, Pier Luigi Fiori
Trichomonas vaginalis is a protozoan with an extracellular obligatory parasitic lifestyle exclusively adapted to the human urogenital tract and responsible for nearly a quarter billion sexually transmitted infections worldwide each year. This review focuses on symbiotic Trichomonasvirus and mycoplasma carried by the protozoan, their molecular features and their role in altering the human vaginal microbiome and the immunopathogenicity of the parasite. Improved diagnostics and larger clinical interventional studies are needed to confirm the causative role of protozoan symbionts in the variable clinical presentation of trichomoniasis and its morbid sequelae, including adverse reproductive outcome, susceptibility to viral infections and cancer...
March 31, 2017: Research in Microbiology
C Corral-Vázquez, R Aguilar-Quesada, P Catalina, G Lucena-Aguilar, G Ligero, B Miranda, J A Carrillo-Ávila
Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process...
June 2017: Cell and Tissue Banking
R Wang, X C Bian, Y Q Liu, J G Fang, H Wang, H G Liu, M Lian, H Z Ma, L Feng, H Z Wang
Objective: To establish a laryngeal squamous cell carcinoma (LSCC) cell line through primary cell culture and observe its biological characteristics. Methods: Tissue block culture method was used for primary cell culture. After LSCC cells passed 25 times in vitro, the morphology of cells was observed, keratin was stained histochemically, cell cycle was tested by PI-FACS, and the specie of cells was detected by PCR and short tandem repeat(STR) typing. Results: This newly established LSCC cell line was named as TR-LCC-1, most of the cancer cells were polygonal shape, like the cobblestone, loss of contact inhibition and with overlapping growth...
January 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
M Lu, Y H Chen, X Han, W Yang, J Zhang, X Zhu, H Zhu, B He, B Z Sun, W Z Yao
Objective: To describe diseases that may present with the reversed halo sign (RHS) on high-resolution CT, and therefore to improve the recognition of this thoracic radiological feature. Methods: We retrospectively reviewed the HRCT scans and medical records of 5 patients who presented with RHS on chest CT from Peking University Third Hospital. With "reversed halo sign, fairy ring, atoll sign" as the Chinese and English keywords, 420 cases from the literatures were also reviewed through PubMed, Wanfang and CNKI databases until August 2015...
October 12, 2016: Chinese Journal of Tuberculosis and Respiratory Diseases
Jun Wu, Lijuan Wu, Cheng Fang, Rong Nie, Jiamou Wang, Xuan Wang, Wenbin Liu
Mycoplasmas are a group of microbes that can cause human diseases. The mycoplasmal lipoprotein p37 promotes cancer metastasis, at least in part, by interacting with EGFR. In this study, we show that the p37 lipoprotein binds another member of the EGFR family, HER2, through the HER2 extracellular domain. The binding of p37-HER2 promotes phosphorylation of HER2 and activates the downstream signaling molecule Erk1/2. Because the HER2 signaling pathway contributes to breast tumor metastasis, our results imply that the mycoplasmal lipoprotein p37 may also be involved in breast cancer metastasis...
November 2016: Microbiological Research
Frances Mercer, Fitz Gerald I Diala, Yi-Pei Chen, Brenda M Molgora, Shek Hang Ng, Patricia J Johnson
Trichomonas vaginalis (Tv) is an extracellular protozoan parasite that causes the most common non-viral sexually transmitted infection: trichomoniasis. While acute symptoms in women may include vaginitis, infections are often asymptomatic, but can persist and are associated with medical complications including increased HIV susceptibility, infertility, pre-term labor, and higher incidence of cervical cancer. Heightened inflammation resulting from Tv infection could account for these complications. Effective cellular immune responses to Tv have not been characterized, and re-infection is common, suggesting a dysfunctional adaptive immune response...
August 2016: PLoS Neglected Tropical Diseases
André Lp de Abreu, Natália Malaguti, Raquel P Souza, Nelson S Uchimura, Érika C Ferreira, Monalisa W Pereira, Maria Db Carvalho, Sandra M Pelloso, Marcelo G Bonini, Fabrícia Gimenes, Marcia El Consolaro
The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods...
2016: American Journal of Cancer Research
Nicole Strittmatter, Anna Lovrics, Judit Sessler, James S McKenzie, Zsolt Bodai, M Luisa Doria, Nora Kucsma, Gergely Szakacs, Zoltan Takats
Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin...
August 2, 2016: Analytical Chemistry
Jieping Chen, Kai Yao, Zaishang Li, Chuangzhong Deng, Liangjiao Wang, Xingsu Yu, Peili Liang, Qiankun Xie, Peng Chen, Zike Qin, Yunlin Ye, Zhuowei Liu, Fangjian Zhou, Zhenfeng Zhang, Hui Han
PURPOSE: To establish penile cancer (PeCa) cell lines for the study of molecular mechanisms of carcinogenesis and testing therapeutic reagents. MATERIALS AND METHODS: We successfully established two PeCa cell lines from fresh tumor tissues from 21 cases. One cell line named Penl1 was isolated from a lymph node metastasis (LNM) of penile squamous cell carcinoma (PeSCC), usual type and comprehensively characterized here. Our in-depth characterization analysis of the Penl1 cell line included morphology, tumorigenicity, genetic characteristics, protein expression, biology, and chemosensitivity...
August 9, 2016: Oncotarget
(no author information available yet)
No abstract text is available yet for this article.
2016: PloS One
Shahanavaj Khan, Mohammed Zakariah, Christian Rolfo, Lembrechts Robrecht, Sellappan Palaniappan
Although the idea of bacteria causing different types of cancer has exploded about century ago, the potential mechanisms of carcinogenesis is still not well established. Many reports showed the involvement of M. hominis in the development of prostate cancer, however, mechanistic approach for growth and development of prostate cancer has been poorly understood. In the current study, we predicted M. hominis proteins targeting in the mitochondria and cytoplasm of host cells and their implication in prostate cancer...
May 9, 2017: Oncotarget
Qiyan Xiong, Jia Wang, Yan Ji, Bo Ni, Bixiong Zhang, Qinghong Ma, Yanna Wei, Shaobo Xiao, Zhixin Feng, Maojun Liu, Guoqing Shao
Mycoplasma hyorhinis (M. hyorhinis) is a swine pathogen that is associated with various human cancers and contamination in cell cultures. However, no studies on the adhesion molecules of this pathogen have yet been reported. The variable lipoprotein (Vlp) family is an important surface component of M. hyorhinis. Herein, we performed several experiments to identify the function of the Vlp family in adherence to host cells. Seven recombinant Vlp (rVlp) proteins were expressed in Escherichia coli and purified by affinity chromatography...
April 15, 2016: Veterinary Microbiology
Daria A Burmistrova, Sergey V Tillib, Dmitry V Shcheblyakov, Inna V Dolzhikova, Dmitry N Shcherbinin, Olga V Zubkova, Tatiana I Ivanova, Amir I Tukhvatulin, Maxim M Shmarov, Denis Y Logunov, Boris S Naroditsky, Aleksandr L Gintsburg
Developing pathogen-specific recombinant antibody fragments (especially nanobodies) is a very promising strategy for the treatment of infectious disease. Nanobodies have great potential for gene therapy application due to their single-gene nature. Historically, Mycoplasma hominis has not been considered pathogenic bacteria due to the lack of acute infection and partially due to multiple studies demonstrating high frequency of isolation of M. hominis samples from asymptomatic patients. However, recent studies on the role of latent M...
2016: PloS One
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