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Escherichia coli nissle 1917

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https://www.readbyqxmd.com/read/28398304/engineered-probiotic-escherichia-coli-can-eliminate-and-prevent-pseudomonas-aeruginosa-gut-infection-in-animal-models
#1
In Young Hwang, Elvin Koh, Adison Wong, John C March, William E Bentley, Yung Seng Lee, Matthew Wook Chang
Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice)...
April 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/28396664/unraveling-the-differences-between-gram-positive-and-gram-negative-probiotics-in-modulating-protective-immunity-to-enteric-infections
#2
REVIEW
Sukumar Kandasamy, Anastasia N Vlasova, David D Fischer, Kuldeep S Chattha, Lulu Shao, Anand Kumar, Stephanie N Langel, Abdul Rauf, Huang-Chi Huang, Gireesh Rajashekara, Linda J Saif
The role of intestinal microbiota and probiotics in prevention and treatment of infectious diseases, including diarrheal diseases in children and animal models, is increasingly recognized. Intestinal commensals play a major role in development of the immune system in neonates and in shaping host immune responses to pathogens. Lactobacilli spp. and Escherichia coli Nissle 1917 are two probiotics that are commonly used in children to treat various medical conditions including human rotavirus diarrhea and inflammatory bowel disease...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28368910/iron-supplements-modulate-colon-microbiota-composition-and-potentiate-the-protective-effects-of-probiotics-in-dextran-sodium-sulfate-induced-colitis
#3
Marco Constante, Gabriela Fragoso, Joseph Lupien-Meilleur, Annie Calvé, Manuela M Santos
BACKGROUND: Iron is an important nutrient for both the host and colonizing bacteria. Oral iron supplementation may impact the composition of the microbiota and can be particularly damaging to patients suffering from inflammatory bowel disease (IBD). However, patients with IBD may require iron supplementation to treat their anemia. METHODS: We fed mice with diets supplemented with ferrous sulfate at different doses (5, 50, and 500 mg of iron/kg chow) and with different iron formulations (ferrous sulfate, ferrous bisglycinate and ferric ethylenediaminetetraacetic acid [FEDTA]), and analyzed the effects on the composition of the gut microbiota by 16S ribosomal RNA gene sequencing...
May 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28337589/colonization-by-non-pathogenic-bacteria-alters-mrna-expression-of-cytochromes-p450-in-originally-germ-free-mice
#4
L Jourová, P Anzenbacher, B Lišková, Z Matušková, P Hermanová, T Hudcovic, H Kozáková, L Hrnčířová, E Anzenbacherová
Gut microbiota provides a wide range of beneficial function for the host and has an immense effect on the host's health state. It has also been shown that gut microbiome is often involved in the biotransformation of xenobiotics; however, the molecular mechanisms of the interaction between the gut bacteria and the metabolism of drugs by the host are still unclear. To investigate the effect of microbial colonization on messenger RNA (mRNA) expression of liver cytochromes P450 (CYPs), the main drug-metabolizing enzymes, we used germ-free (GF) mice, lacking the intestinal flora and mice monocolonized by non-pathogenic bacteria Lactobacillus plantarum (NIZO2877) or probiotic bacteria Escherichia coli Nissle 1917 compared to specific pathogen-free (SPF) mice...
March 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28283693/genetically-engineered-escherichia-coli-nissle-1917-synbiotic-counters-fructose-induced-metabolic-syndrome-and-iron-deficiency
#5
Archana Somabhai Chaudhari, Ruma Raghuvanshi, G Naresh Kumar
Consumption of fructose leads to metabolic syndrome, but it is also known to increase iron absorption. Present study investigates the effect of genetically modified Escherichia coli Nissle 1917 (EcN) synbiotic along with fructose on non-heme iron absorption. Charles foster rats weighing 150-200 g were fed with iron-deficient diet for 2 months. Probiotic treatment of EcN (pqq) and EcN (pqq-glf-mtlK) was given once per week, 10(9) cells after 2 months with fructose in drinking water. Iron levels, blood, and liver parameters for oxidative stress, hyperglycemia, and dyslipidemia were estimated...
March 10, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28172826/p115-escherichia-coli-nissle-1917-modulate-gut-microbiota-composition-in-ulcerative-colitis-patients
#6
C Graziani, V Petito, F Del Chierico, F Mangiola, S Pecere, E Schiavoni, M Pizzoferrato, L R Lopetuso, L Putignani, A Gasbarrini, F Scaldaferri
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28172737/p116-escherichia-coli-nissle-1917-distinctively-regulates-apoptosis-and-cell-cycling-of-caco-2-cells
#7
A Schirbel, A Sturm
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28094590/effects-of-supernatants-from-escherichia-coli-nissle-1917-and-faecalibacterium-prausnitzii-on-intestinal-epithelial-cells-and-a-rat-model-of-5-fluorouracil-induced-mucositis
#8
Hanru Wang, Yoga D Jatmiko, Susan E P Bastian, Suzanne Mashtoub, Gordon S Howarth
Faecalibacterium prausnitzii (Fp) and Escherichia coli Nissle 1917 (EcN) are probiotics, which have been reported to ameliorate certain gastrointestinal disorders. We evaluated the effects of supernatants (SN) derived from Fp and EcN on 5-fluorouracil (5-FU)-treated intestinal cells and in a rat model of mucositis. In vitro: IEC-6, Caco-2, and T-84 cells were analyzed for viability and monolayer permeability. In vivo: Female dark agouti rats were gavaged with Fp or EcN SN and injected intraperitoneally with saline (control) or 5-FU to induce mucositis...
February 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28069496/probiotic-e-coli-nissle-1917-biofilms-on-silicone-substrates-for-bacterial-interference-against-pathogen-colonization
#9
Quan Chen, Zhiling Zhu, Jun Wang, Analette I Lopez, Siheng Li, Amit Kumar, Fei Yu, Haoqing Chen, Chengzhi Cai, Lijuan Zhang
Bacterial interference is an alternative strategy to fight against device-associated bacterial infections. Pursuing this strategy, a non-pathogenic bacterial biofilm is used as a live, protective barrier to fence off pathogen colonization. In this work, biofilms formed by probiotic Escherichia coli strain Nissle 1917 (EcN) are investigated for their potential for long-term bacterial interference against infections associated with silicone-based urinary catheters and indwelling catheters used in the digestive system, such as feeding tubes and voice prostheses...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28018313/outer-membrane-vesicles-and-soluble-factors-released-by-probiotic-escherichia-coli-nissle-1917-and-commensal-ecor63-enhance-barrier-function-by-regulating-expression-of-tight-junction-proteins-in-intestinal-epithelial-cells
#10
Carina-Shianya Alvarez, Josefa Badia, Manel Bosch, Rosa Giménez, Laura Baldomà
The gastrointestinal epithelial layer forms a physical and biochemical barrier that maintains the segregation between host and intestinal microbiota. The integrity of this barrier is critical in maintaining homeostasis in the body and its dysfunction is linked to a variety of illnesses, especially inflammatory bowel disease. Gut microbes, and particularly probiotic bacteria, modulate the barrier integrity by reducing gut permeability and reinforcing tight junctions. Probiotic Escherichia coli Nissle 1917 (EcN) is a good colonizer of the human gut with proven therapeutic efficacy in the remission of ulcerative colitis in humans...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27999620/effects-of-escherichia-coli-nissle-1917-and-ciprofloxacin-on-small-intestinal-epithelial-cell-mrna-expression-in-the-neonatal-piglet-model-of-human-rotavirus-infection
#11
Francine C Paim, Stephanie N Langel, David D Fischer, Sukumar Kandasamy, Lulu Shao, Moyasar A Alhamo, Huang-Chi Huang, Anand Kumar, Gireesh Rajashekara, Linda J Saif, Anastasia N Vlasova
We evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) and the antibiotic Ciprofloxacin (Cipro) on mRNA expression of intestinal epithelial cells (IEC) in gnotobiotic (Gn) piglets colonized with a defined commensal microflora (DMF) and inoculated with human rotavirus (HRV) that infects IECs. We analyzed mRNA levels of IEC genes for enteroendocrine cells [chromogranin A (CgA)], goblet cells [mucin 2 (MUC2)], transient amplifying progenitor cell [proliferating cell nuclear antigen (PCNA)], intestinal epithelial stem cell (SOX9) and enterocytes (villin)...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27853451/high-protective-efficacy-of-probiotics-and-rice-bran-against-human-norovirus-infection-and-diarrhea-in-gnotobiotic-pigs
#12
Shaohua Lei, Ashwin Ramesh, Erica Twitchell, Ke Wen, Tammy Bui, Mariah Weiss, Xingdong Yang, Jacob Kocher, Guohua Li, Ernawati Giri-Rachman, Nguyen Van Trang, Xi Jiang, Elizabeth P Ryan, Lijuan Yuan
Probiotics have been recognized as vaccine adjuvants and therapeutic agents to treat acute gastroenteritis in children. We previously showed that rice bran (RB) reduced human rotavirus diarrhea in gnotobiotic pigs. Human noroviruses (HuNoVs) are the major pathogens causing non-bacterial acute gastroenteritis worldwide. In this study, Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle 1917 (EcN) were first screened for their ability to bind HuNoV P particles and virions derived from clinical samples containing HuNoV genotype GII...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27798599/microcins-mediate-competition-among-enterobacteriaceae-in-the-inflamed-gut
#13
Martina Sassone-Corsi, Sean-Paul Nuccio, Henry Liu, Dulcemaria Hernandez, Christine T Vu, Amy A Takahashi, Robert A Edwards, Manuela Raffatellu
The Enterobacteriaceae are a family of Gram-negative bacteria that include commensal organisms as well as primary and opportunistic pathogens that are among the leading causes of morbidity and mortality worldwide. Although Enterobacteriaceae often comprise less than 1% of a healthy intestine's microbiota, some of these organisms can bloom in the inflamed gut; expansion of enterobacteria is a hallmark of microbial imbalance known as dysbiosis. Microcins are small secreted proteins that possess antimicrobial activity in vitro, but whose role in vivo has been unclear...
December 8, 2016: Nature
https://www.readbyqxmd.com/read/27795372/escherichia-coli-probiotic-strain-ed1a-in-pigs-has-a-limited-impact-on-the-gut-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-e-coli
#14
G Mourand, F Paboeuf, M A Fleury, E Jouy, S Bougeard, E Denamur, I Kempf
Four trials were conducted to evaluate the impact of Escherichia coli probiotic strain ED1a administration to pigs on the gut carriage or survival in manure of extended-spectrum-β-lactamase-producing E. coli Groups of pigs were orally inoculated with strain E. coli M63 carrying the blaCTX-M-1 gene (n = 84) or used as a control (n = 26). In the first two trials, 24 of 40 E. coli M63-inoculated pigs were given E. coli ED1a orally for 6 days starting 8 days after oral inoculation. In the third trial, 10 E. coli M63-inoculated pigs were given either E...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27782051/pmpes-a-modular-peptide-expression-system-for-the-delivery-of-antimicrobial-peptides-to-the-site-of-gastrointestinal-infections-using-probiotics
#15
Kathryn Geldart, Brittany Forkus, Evelyn McChesney, Madeline McCue, Yiannis N Kaznessis
Antimicrobial peptides are a promising alternative to traditional antibiotics, but their utility is limited by high production costs and poor bioavailability profiles. Bacterial production and delivery of antimicrobial peptides (AMPs) directly at the site of infection may offer a path for effective therapeutic application. In this study, we have developed a vector that can be used for the production and secretion of seven antimicrobial peptides from both Escherichia coli MC1061 F' and probiotic E.coli Nissle 1917...
October 5, 2016: Pharmaceuticals
https://www.readbyqxmd.com/read/27766164/insights-from-100-years-of-research-with-probiotic-e-coli
#16
REVIEW
Trudy M Wassenaar
A century ago, Alfred Nissle discovered that intentional intake of particular strains of Escherichia coli could treat patients suffering from infectious diseases. Since then, one of these strains became the most frequently used probiotic E. coli in research and was applied to a variety of human conditions. Here, properties of that E. coli Nissle 1917 strain are compared with other commercially available E. coli probiotic strains, with emphasis on their human applications. A literature search formed the basis of a summary of research findings reported for the probiotics Mutaflor, Symbioflor 2, and Colinfant...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27760187/genetically-engineered-escherichia-coli-nissle-1917-synbiotics-reduce-metabolic-effects-induced-by-chronic-consumption-of-dietary-fructose
#17
Chaudhari Archana Somabhai, Ruma Raghuvanshi, G Nareshkumar
AIMS: To assess protective efficacy of genetically modified Escherichia coli Nissle 1917 (EcN) on metabolic effects induced by chronic consumption of dietary fructose. MATERIALS AND METHODS: EcN was genetically modified with fructose dehydrogenase (fdh) gene for conversion of fructose to 5-keto-D-fructose and mannitol-2-dehydrogenase (mtlK) gene for conversion to mannitol, a prebiotic. Charles foster rats weighing 150-200 g were fed with 20% fructose in drinking water for two months...
2016: PloS One
https://www.readbyqxmd.com/read/27696378/probiotics-fiber-and-herbal-medicinal-products-for-functional-and-inflammatory-bowel-disorders
#18
Diego Currò, Gianluca Ianiro, Silvia Pecere, Stefano Bibbò, Giovanni Cammarota
Functional bowel disorders (FBDs), mainly irritable bowel syndrome (IBS) and functional constipation (FC, also called chronic idiopathic constipation), are highly diffused worldwide. Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, although less common, has a strong impact on patients' quality of life, as well as is highly expensive for our healthcare. A definite cure for those disorders is still yet to come. Over the years, several therapeutic approaches complementary or alternative to traditional pharmacological treatments, including probiotics, prebiotics, synbiotics, fiber and herbal medicinal products, have been investigated for the management of both groups of diseases...
October 3, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27640192/genetic-engineering-of-probiotic-escherichia-coli-nissle-1917-for-clinical-application
#19
REVIEW
Bingming Ou, Ying Yang, Wai Liang Tham, Lin Chen, Jitao Guo, Guoqiang Zhu
Escherichia coli strain Nissle 1917 (EcN) has been used as a probiotic. Genetic engineering has enhanced the utility of EcN in several vaccine and pharmaceutical preparations. We discuss in this mini review the genetics and physical properties of EcN. We also discuss the numerous genetic engineering strategies employed for EcN-based vaccine development, including recombinant plasmid transfer, genetic engineering of cryptic plasmids or the EcN chromosome, EcN bacterial ghosts and its outer membrane vesicles...
October 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27619890/escherichia-coli-strain-nissle-1917-from-bench-to-bedside-and-back-history-of-a-special-escherichia-coli-strain-with-probiotic-properties
#20
Ulrich Sonnenborn
Among the gram-negative microorganisms with probiotic properties, Escherichia coli strain Nissle 1917 (briefly EcN) is probably the most intensively investigated bacterial strain today. Since nearly 100 years, the EcN strain is used as the active pharmaceutical ingredient in a licensed medicinal product that is distributed in Germany and several other countries. Over the last few decades, novel probiotic activities have been detected, which taken together are specific of this versatile E. coli strain. This review gives a short overview on the discovery and history of the EcN strain...
October 2016: FEMS Microbiology Letters
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