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https://www.readbyqxmd.com/read/28932858/self-assembled-clusters-of-patchy-rod-like-molecules
#1
Miroslav Jurásek, Robert Vácha
The design of complex self-assembled structures remains a challenging task due to the intricate relationship between the properties of the building blocks and the final morphology of the aggregates. Here, we report such a relationship for rod-like particles with one or two attractive patches based on a combination of computer simulations and analytical theory. We investigated the formation of finite aggregates under various conditions and constructed structure diagrams, which can be used to determine and extrapolate the system composition...
September 21, 2017: Soft Matter
https://www.readbyqxmd.com/read/28932249/the-novel-protein-c3orf43-accelerates-hepatocyte-proliferation
#2
Chunyan Zhang, Cuifang Chang, Deming Li, Fuchun Zhang, Cunshuan Xu
BACKGROUND: Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its physiological functions remains unclear. METHODS: The expressions of C3orf43 in BRL-3A cells transfected with C3orf43-siRNA (C3-siRNA) or overexpressing the vector plasmid pCDH-C3orf43 (pCDH-C3) were measured via RT-qPCR and western blot...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28931265/diagnostic-and-interventional-optical-angioscopy-in-ex-vivo-carotid-arteries
#3
Luis E Savastano, Neeraj Chaudhary, Carlos Murga-Zamalloa, Michael Wang, Thomas Wang, B Gregory Thompson
BACKGROUND: Angioscopy - or endovascular endoscopy - is a catheter-based technique employing a flexible fiberoptic angioscope to directly visualize arterial lumen. Poor resolution and excessive stiffness of pre-existent angioscopes limited their use clinically. Recent advances resulted in novel fused optical fiber bundle angioscopes with improved flexibility and imaging resolution. Use of these devices in endovascular neurosurgery is still largely unexplored. OBJECTIVE: To evaluate image quality and feasibility of optical angioscopes for diagnostic and interventional neuro-angioscopy in carotid arteries of human cadavers...
February 1, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/28931061/binding-of-a-c-type-lectin-s-coiled-coil-domain-to-the-domeless-receptor-directly-activates-the-jak-stat-pathway-in-the-shrimp-immune-response-to-bacterial-infection
#4
Jie-Jie Sun, Jiang-Feng Lan, Xiao-Fan Zhao, Gerardo R Vasta, Jin-Xing Wang
C-type lectins (CTLs) are characterized by the presence of a C-type carbohydrate recognition domain (CTLD) that by recognizing microbial glycans, is responsible for their roles as pattern recognition receptors in the immune response to bacterial infection. In addition to the CTLD, however, some CTLs display additional domains that can carry out effector functions, such as the collagenous domain of the mannose-binding lectin. While in vertebrates, the mechanisms involved in these effector functions have been characterized in considerable detail, in invertebrates they remain poorly understood...
September 20, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28927691/comparative-genomics-of-four-mycoplasma-species-of-the-human-urogenital-tract-analysis-of-their-core-genomes-and-virulence-genes
#5
Orville St E Roachford, Karen E Nelson, Bidyut R Mohapatra
The variation in Mycoplasma lipoproteins attributed to genome rearrangements and genetic insertions leads to phenotypic plasticity that allows for the evasion of the host's defence system and pathogenesis. This paper compared for the first time the genomes of four human urogenital Mycoplasma species (M. penetrans HF-2, M. fermentans JER, M. genitalium G37 and M. hominis PG21) to categorise the metabolic functions of the core genes and to assess the effects of tandem repeats, phage-like genetic elements and prophages on the virulence genes...
September 5, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28925256/catalytic-activity-of-peptide-nanoparticle-conjugates-regulated-by-a-conformational-change
#6
Dorian Jamal Mikolajczak, Jason Lee Heier, Boris Schade, Beate Koksch
Herein, we present the design and synthesis of a catalytically active peptide-nanoparticle conjugate whose activity is regulated by a defined conformational change in the self-assembled peptide monolayer. A catalytically active peptide, designed after the heterodimeric α-helical coiled-coil principle was immobilized onto gold nanoparticles and kinetic studies were performed according to the Michaelis-Menten model. The formed peptide monolayer at the gold nanoparticle surface accelerated p nitrophenylacetate (pNPA) hydrolysis by one order of magnitude compared to the soluble peptide while exhibiting no defined secondary structure as determined by infrared (IR) and circular dichroism (CD) spectroscopy...
September 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28922746/ectopic-expression-of-winding-1-leads-to-asymmetrical-distribution-of-auxin-and-a-spiral-phenotype-in-rice
#7
Ming-Lung Cheng, Shuen-Fang Lo, An-Shan Hsiao, Ya-Fang Hong, Su-May Yu, Tuan-Hua David Ho
Ectopic expression of the rice WINDING 1 (WIN1) gene leads to a spiral phenotype only in shoots but not in roots. Rice WIN1 belongs to a specific class of proteins in cereal plants containing a Bric-a-Brac/Tramtrack/Broad (BTB) complex, a non-phototropic hypocotyl 3 (NPH3) domain and a coiled-coil motif. The WIN1 protein is predominantly localized to the plasma membrane, but is also co-localized to plasmodesmata, where it exhibits a punctate pattern. It is observed that WIN1 is normally expressed in roots and the shoot-root junction, but not in the rest of shoots...
September 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28922450/structural-insight-into-the-xtacc3-xmap215-interaction-from-cd-and-nmr-studies-on-model-peptides
#8
Angélica Partida-Hanon, Miguel A Treviño, Miguel Mompeán, Ma Ángeles Jiménez, Marta Bruix
TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled-coil model for this interaction is available but the structural details are not well described. To characterize this interaction at atomic resolution, we have designed a simplified version of the system based on small peptides. Four different peptides have been studied by circular dichroism and nuclear magnetic resonance both singly and in all possible combinations; namely, five peptide pairs and two trios...
September 18, 2017: Biopolymers
https://www.readbyqxmd.com/read/28921867/polymer-cancerostatics-targeted-with-an-antibody-fragment-bound-via-a-coiled-coil-motif-in-vivo-therapeutic-efficacy-against-murine-bcl1-leukemia
#9
Michal Pechar, Robert Pola, Olga Janoušková, Irena Sieglová, Vlastimil Král, Milan Fábry, Barbora Tomalová, Marek Kovář
A BCL1 leukemia-cell-targeted polymer-drug conjugate with a narrow molecular weight distribution consisting of an N-(2-hydroxypropyl)methacrylamide copolymer carrier and the anticancer drug pirarubicin is prepared by controlled radical copolymerization followed by metal-free click chemistry. A targeting recombinant single chain antibody fragment (scFv) derived from a B1 monoclonal antibody is attached noncovalently to the polymer carrier via a coiled coil interaction between two complementary peptides. Two pairs of coiled coil forming peptides (abbreviated KEK/EKE and KSK/ESE) are used as linkers between the polymer-pirarubicin conjugate and the targeting protein...
September 15, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28919443/fv-clasp-an-artificially-designed-small-antibody-fragment-with-improved-production-compatibility-stability-and-crystallizability
#10
Takao Arimori, Yu Kitago, Masataka Umitsu, Yuki Fujii, Ryoko Asaki, Keiko Tamura-Kawakami, Junichi Takagi
Antibody fragments are frequently used as a "crystallization chaperone" to aid structural analysis of complex macromolecules that are otherwise crystallization resistant, but conventional fragment formats have not been designed for this particular application. By fusing an anti-parallel coiled-coil structure derived from the SARAH domain of human Mst1 kinase to the variable region of an antibody, we succeeded in creating a novel chimeric antibody fragment of ∼37 kDa, termed "Fv-clasp," which exhibits excellent crystallization compatibility while maintaining the binding ability of the original IgG molecule...
September 6, 2017: Structure
https://www.readbyqxmd.com/read/28916358/the-functional-characterization-and-comparison-of-two-single-crd-containing-c-type-lectins-with-novel-and-typical-key-motifs-from-portunus-trituberculatus
#11
Mengmeng Huang, Changkao Mu, Yuehong Wu, Fei Ye, Dan Wang, Cong Sun, Zhengbing Lv, Bingnan Han, Chunlin Wang, Xue-Wei Xu
C-type lectins are a superfamily of Ca(2+)-dependent carbohydrate-recognition proteins, which play crucial roles in innate immunity including nonself-recognition and pathogen elimination. In the present study, two single-CRD containing C-type lectins were identified from swimming crab Portunus trituberculatus (designated as PtCTL-2 and PtCTL-3). The open reading frame (ORF) of PtCTL-2 encoded polypeptides of 485 amino acids with a signal peptide and a single carbohydrate-recognition domain (CRD), while PtCTL-3's ORF encoded polypeptides of 241 amino acids with a coiled-coil region and a single-CRD...
September 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28913864/strand-displacement-in-coiled-coil-structures-controlled-induction-and-reversal-of-proximity
#12
Katharina Gröger, Georgina Gavins, Oliver Seitz
Coiled-coil peptides are frequently used to create new function upon self-assembly of supramolecular complexes. A multitude of coil peptide sequences provides control over specificity and stability of coiled-coil complexes. However, comparably little attention has been paid to the development of methods that allow for the reversal of complex formation under non-denaturing conditions. Herein, we present a reversible 2-state switching system. The process involves two peptide molecules for formation of a size-mismatched coiled-coil duplex and a third, disruptor peptide which targets an overhanging end...
September 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28912033/dissecting-the-cd93-multimerin-2-interaction-involved-in-cell-adhesion-and-migration-of-the-activated-endothelium
#13
Federico Galvagni, Federica Nardi, Ottavia Spiga, Alfonso Trezza, Giulia Tarticchio, Rosanna Pellicani, Eva Andreuzzi, Elena Caldi, Paolo Toti, Gian Marco Tosi, Annalisa Santucci, Renato V Iozzo, Maurizio Mongiat, Maurizio Orlandini
The glycoprotein CD93 has recently been recognized to play an important role in the regulation of the angiogenic process. Moreover, CD93 is highly expressed in the endothelial cells of tumor blood vessel and faintly expressed in the non-proliferating endothelium. Much evidence suggests that CD93 mediates adhesion in the endothelium. Here we identify Multimerin 2 (MMRN2), a pan-endothelial extracellular matrix protein, as a specific ligand for CD93. We found that CD93 and MMRN2 are co-expressed in the blood vessels of various human tumors...
September 11, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28903364/kif7-attenuates-prostate-tumor-growth-through-lkb1-mediated-akt-inhibition
#14
Kai Yau Wong, Jing Liu, Kwok Wah Chan
This study investigated kinesin family member 7 (KIF7) expression and function in prostate cancer (PCa). Our results showed that KIF7 was significantly downregulated in PCa, compared with normal, benign prostatic hyperplasia and prostate intraepithelial neoplasia tissues, partially through promoter hypermethylation. We further investigated the effects of KIF7 coiled coil (CC) domain and motor domain (MD) on PCa development in vitro and in vivo. Our results showed that KIF7-CC but not KIF7-MD significantly attenuated proliferation and colony formation, impeded migration and invasion, induced apoptosis and sensitized PCa cells to paclitaxel...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28900907/the-stim-orai-pathway-stim-orai-structures-isolated-and-in-complex
#15
Jinhui Zhu, Qingping Feng, Peter B Stathopulos
Considerable progress has been made elucidating the molecular mechanisms of calcium (Ca(2+)) sensing by stromal interaction molecules (STIMs) and the basis for Orai channel activity. This chapter focuses on the available high-resolution structural details of STIM and Orai proteins with respect to the regulation of store-operated Ca(2+) entry (SOCE). Solution structures of the Ca(2+)-sensing domains of STIM1 and STIM2 are reviewed in detail, crystal structures of cytosolic coiled-coil STIM fragments are discussed, and an overview of the closed Drosophila melanogaster Orai hexameric structure is provided...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28898068/protein-mediated-colloidal-assembly
#16
Maiko Obana, Bradley Ross Silverman, David A Tirrell
Programmable colloidal assembly enables the creation of mesoscale materials in a bottom-up manner. Although DNA oligonucleotides have been used extensively as the programmable units in this paradigm, proteins, which exhibit more diverse modes of association and function, have not been widely used to direct colloidal assembly. Here we use protein-protein interactions to drive controlled aggregation of polystyrene microparticles, either through reversible coiled-coil interactions or through intermolecular isopeptide linkages...
September 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28895065/structural-characterization-of-the-trimerization-of-traf6-protein-through-molecular-dynamics-simulations
#17
Ria Biswas, Angshuman Bagchi
The tumour necrosis factor (TNF) receptor-associated factor (TRAF) family of proteins having E3 ligase activity are the key molecules involved in cellular immune response pathways. TRAF6 is a unique member of the TRAF superfamily differing from other members of the family, owing to its specific interactions with molecules outside the TNF receptor superfamily. The C-terminal domain of TRAF proteins contains the catalytic residues and are known to be involved in self-oligomerization forming a mushroom-shaped trimeric structure, which is the functional form of the protein...
September 11, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/28893857/changes-in-dynamic-lung-mechanics-after-lung-volume-reduction-coil-treatment-of-severe-emphysema
#18
Demosthenes Makris, Sylvie Leroy, Johana Pradelli, Jonathan Benzaquen, Hervé Guenard, Jeanne-Marie Perotin, Spyros Zakynthinos, Epaminondas Zakynthinos, Gaëtan Deslee, Charles Hugo Marquette
We assessed the relationships between changes in lung compliance, lung volumes and dynamic hyperinflation in patients with emphysema who underwent bronchoscopic treatment with nitinol coils (coil treatment) (n=11) or received usual care (UC) (n=11). Compared with UC, coil treatment resulted in decreased dynamic lung compliance (CLdyn) (p=0.03) and increased endurance time (p=0.010). The change in CLdyn was associated with significant improvement in FEV1 and FVC, with reduction in residual volume and intrinsic positive end-expiratory pressure, and with increased inspiratory capacity at rest/and at exercise...
September 11, 2017: Thorax
https://www.readbyqxmd.com/read/28893745/a-new-risk-locus-in-chchd5-for-hypertension-and-obesity-in-a-chinese-child-population-a-cohort-study
#19
Lijun Wu, Liwang Gao, Xiaoyuan Zhao, Meixian Zhang, Jianxin Wu, Jie Mi
OBJECTIVE: Coiled-coil-helix-coiled-coil-helix domain containing 5 (CHCHD5), a mitochondrial protein, is involved in the oxidative folding process in the mitochondrial intermembrane space. A previous study identified a hypertension-related single nucleotide polymorphism (SNP), rs3748024, in CHCHD5 in adults, but there are no reports regarding the association between CHCHD5 and obesity, which is a known risk factor for hypertension. The aim of the present study is to investigate the associations of the SNP rs3748024 with hypertension and obesity...
September 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28887874/small-molecule-inhibition-of-murf1-attenuates-skeletal-muscle-atrophy-and-dysfunction-in-cardiac-cachexia
#20
Thomas Scott Bowen, Volker Adams, Sarah Werner, Tina Fischer, Paulien Vinke, Maria Noel Brogger, Norman Mangner, Axel Linke, Peter Sehr, Joe Lewis, Dittmar Labeit, Alexander Gasch, Siegfried Labeit
BACKGROUND: Muscle ring finger 1 (MuRF1) is a muscle-specific ubiquitin E3 ligase activated during clinical conditions associated with skeletal muscle wasting. Yet, there remains a paucity of therapeutic interventions that directly inhibit MuRF1 function, particularly in vivo. The current study, therefore, developed a novel compound targeting the central coiled coil domain of MuRF1 to inhibit muscle wasting in cardiac cachexia. METHODS: We identified small molecules that interfere with the MuRF1-titin interaction from a 130 000 compound screen based on Alpha Technology...
September 8, 2017: Journal of Cachexia, Sarcopenia and Muscle
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