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https://www.readbyqxmd.com/read/28521418/overexpression-of-e3-ubiquitin-ligase-tripartite-motif-32-correlates-with-a-poor-prognosis-in-patients-with-gastric-cancer
#1
Masahiro Ito, Kazuhiro Migita, Sohei Matsumoto, Kohei Wakatsuki, Tetsuya Tanaka, Tomohiro Kunishige, Hiroshi Nakade, Mitsuhiro Nakatani, Yoshiyuki Nakajima
Tripartite motif protein (TRIM) 32 belongs to the TRIM family, which is composed of RING finger, B-box and coiled-coil domains. TRIM32 has been reported to function as an enzyme 3 ubiquitin ligase and is overexpressed in numerous types of cancer. The present study evaluated the clinical significance of TRIM32 expression levels in gastric cancer. The current study also investigated the TRIM32 expression levels in 142 patients with gastric cancer using immunohistochemistry and examined its clinical importance and potential as a prognostic marker...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28514735/kif7-attenuates-prostate-tumor-growth-through-lkb1-mediated-akt-inhibition
#2
Kai Yau Wong, Jing Liu, Kwok Wah Chan
This study investigated kinesin family member 7 (KIF7) expression and function in prostate cancer (PCa). Our results showed that KIF7 was significantly downregulated in PCa, compared with normal, benign prostatic hyperplasia and prostate intraepithelial neoplasia tissues, partially through promoter hypermethylation. We further investigated the effects of KIF7 coiled coil (CC) domain and motor domain (MD) on PCa development in vitro and in vivo. Our results showed that KIF7-CC but not KIF7-MD significantly attenuated proliferation and colony formation, impeded migration and invasion, induced apoptosis and sensitized PCa cells to paclitaxel...
April 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28513838/the-emerging-role-of-npnt-in-tissue-injury-repair-and-bone-homeostasis
#3
REVIEW
Youqiang Sun, Vincent Kuek, Heng Qiu, Jennifer Tickner, Leilei Chen, Haibin Wang, Wei He, Jiake Xu
Nephronectin (NPNT), a highly conserved extracellular matrix protein, plays an important role in regulating cell adhesion, differentiation, spreading and survival. NPNT protein belongs to epidermal growth factor (EGF)-like superfamily and exhibits several common structural determinants; including EGF-like repeat domains, MAM domain (Meprin, A5 Protein, and Receptor Protein-Tyrosine Phosphatase µ), RGD motif (Arg-Gly-Asp) and a coiled-coil domain. It regulates integrins-mediated signaling pathways via the interaction of its RGD motif with integrin α8β1...
May 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28512095/structure-based-mutations-in-the-herpes-simplex-virus-1-glycoprotein-b-ectodomain-arm-impart-a-slow-entry-phenotype
#4
Qing Fan, Sarah J Kopp, Sarah A Connolly, Richard Longnecker
Glycoprotein B (gB) is the conserved herpesvirus fusion protein, and it is required for the entry of herpesviruses. The structure of the postfusion conformation of gB has been solved for several herpesviruses; however, the gB prefusion crystal structure and the details of how the protein refolds from a prefusion to a postfusion form to mediate fusion have not been determined. Using structure-based mutagenesis, we previously reported that three mutations (I671A, H681A, and F683A) in the C-terminal arm of the gB ectodomain greatly reduced cell-cell fusion...
May 16, 2017: MBio
https://www.readbyqxmd.com/read/28510759/an-intact-mcm10-coiled-coil-interaction-surface-is-important-for-origin-melting-helicase-assembly-and-the-recruitment-of-pol-%C3%AE-to-mcm2-7
#5
Patricia Perez-Arnaiz, Irina Bruck, Max K Colbert, Daniel L Kaplan
Mcm10 is an essential eukaryotic factor required for DNA replication. The replication fork helicase is composed of Cdc45, Mcm2-7 and GINS (CMG). DDK is an S-phase-specific kinase required for replication initiation, and the DNA primase-polymerase in eukaryotes is pol α. Mcm10 forms oligomers in vitro, mediated by the coiled-coil domain at the N-terminal region of the protein. We characterized an Mcm10 mutant at the N-terminal Domain (NTD), Mcm10-4A, defective for self-interaction. We found that the Mcm10-4A mutant was defective for stimulating DDK phosphorylation of Mcm2, binding to eighty-nucleotide ssDNA, and recruiting pol α to Mcm2-7 in vitro...
May 16, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28508067/cryo-em-structure-of-human-adenovirus-d26-reveals-the-conservation-of-structural-organization-among-human-adenoviruses
#6
Xiaodi Yu, David Veesler, Melody G Campbell, Mary E Barry, Francisco J Asturias, Michael A Barry, Vijay S Reddy
Human adenoviruses (HAdVs) cause acute respiratory, ocular, and gastroenteric diseases and are also frequently used as gene and vaccine delivery vectors. Unlike the archetype human adenovirus C5 (HAdV-C5), human adenovirus D26 (HAdV-D26) belongs to species-D HAdVs, which target different cellular receptors, and is differentially recognized by immune surveillance mechanisms. HAdV-D26 is being championed as a lower seroprevalent vaccine and oncolytic vector in preclinical and human clinical studies. To understand the molecular basis for their distinct biological properties and independently validate the structures of minor proteins, we determined the first structure of species-D HAdV at 3...
May 2017: Science Advances
https://www.readbyqxmd.com/read/28507245/conformational-changes-of-inter-domain-linker-mediate-mechanical-signal-transmission-in-sensor-kinase-bvgs
#7
Elodie Lesne, Elian Dupré, Camille Locht, Rudy Antoine, Françoise Jacob-Dubuisson
The whooping cough agent, Bordetella pertussis, controls the expression of its large virulence regulon in a coordinated manner through the two-component system BvgAS. BvgS is a dimeric, multi-domain sensor-kinase. Each monomer comprises in succession tandem periplasmic Venus flytrap (VFT) domains, a transmembrane segment, a cytoplasmic Per-ArnT-Sim (PAS) domain, a kinase module and additional phosphorelay domains. BvgS shifts between kinase and phosphatase modes of activity in response to chemical modulators that modify the clamshell motions of the VFT domains...
May 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28507241/an-amino-acid-substitution-in-rna-polymerase-that-inhibits-the-utilization-of-an-alternative-sigma-factor
#8
Anna F Wang Erickson, Padraig Deighan, Cinthia P Garcia, Robert O J Weinzierl, Ann Hochschild, Richard Losick
Sigma (σ) factors direct gene transcription by binding to and determining the promoter recognition specificity of RNA polymerase (RNAP) in bacteria. Genes transcribed under the control of alternative sigma factors allow cells to respond to stress and undergo developmental processes such as sporulation in Bacillus subtilis, in which gene expression is controlled by a cascade of alternative sigma factors. Binding of sigma factors to RNA polymerase depends on the coiled-coil (or clamp helices) motif of the β' subunit...
May 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28500526/intermediate-filaments-supporting-cell-shape-and-growth-in-bacteria
#9
Gabriella H Kelemen
For years intermediate filaments (IF), belonging to the third class of filamentous cytoskeletal proteins alongside microtubules and actin filaments, were thought to be exclusive to metazoan cells. Structurally these eukaryote IFs are very well defined, consisting of globular head and tail domains, which flank the central rod-domain. This central domain is dominated by an α-helical secondary structure predisposed to form the characteristic coiled-coil, parallel homo-dimer. These elementary dimers can further associate, both laterally and longitudinally, generating a variety of filament-networks built from filaments in the range of 10 nm in diameter...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28489284/mapping-genotype-phenotype-associations-of-nssnps-in-coiled-coil-oligomerization-domains-of-the-human-proteome
#10
Kaavya A Mohanasundaram, Mani P Grover, Tamsyn M Crowley, Andrzej Goscinski, Merridee A Wouters
We assessed the impact of disease mutations versus polymorphisms in coiled-coil domains in Uniprot by modelling the structural and functional impact of variants in silico with the coiled-coil prediction program Multicoil. The structural impact of variants was evaluated with respect to three main metrics: the oligomerization score-to determine if the variant is stabilising or destabilising, the oligomerization state, and the register-specific score. The functional impact was queried indirectly in several ways...
May 10, 2017: Human Mutation
https://www.readbyqxmd.com/read/28489083/exploring-biomolecular-energy-landscapes
#11
Jerelle A Joseph, Konstantin Röder, Debayan Chakraborty, Rosemary G Mantell, David J Wales
The potential energy landscape perspective provides both a conceptual and a computational framework for predicting, understanding and designing molecular properties. In this Feature Article, we highlight some recent advances that greatly facilitate structure prediction and analysis of global thermodynamics and kinetics in proteins and nucleic acids. The geometry optimisation procedures, on which these calculations are based, can be accelerated significantly using local rigidification of selected degrees of freedom, and through implementations on graphics processing units...
May 10, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28484626/classification-of-self-assembling-protein-nanoparticle-architectures-for-applications-in-vaccine-design
#12
G Indelicato, P Burkhard, R Twarock
We introduce here a mathematical procedure for the structural classification of a specific class of self-assembling protein nanoparticles (SAPNs) that are used as a platform for repetitive antigen display systems. These SAPNs have distinctive geometries as a consequence of the fact that their peptide building blocks are formed from two linked coiled coils that are designed to assemble into trimeric and pentameric clusters. This allows a mathematical description of particle architectures in terms of bipartite (3,5)-regular graphs...
April 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28476675/bioinformatics-and-expression-analysis-of-fintrim-genes-in-grass-carp-ctenopharyngodon-idella
#13
Kai Luo, Youshen Li, Kete Ai, Lihai Xia, Jinxiong Zhang, Wei Hu, Weihua Gao, Liwei Guo, Zhitao Qi, Hanwen Yuan, Qiaoqing Xu
The tripartite motifs (TRIMs) constitute a large family of proteins containing a Really Interesting New Gene (RING) domain, a B-box domain and coiled-coil region followed by different C-terminal domains. TRIM proteins play multiple roles in various cellular processes, including cell growth, differentiation, apoptosis and antiviral immunity. Fish novel large multigene TRIM genes (finTRIM/ftr) appear only in teleosts and play a vital role in antiviral responses. Phylogenetic analysis revealed the existence of different subsets of novel fish TRIM 14 genes (finTRIM14/ftr14), ftr51, ftr67, ftr72, ftr82, ftr83, and ftr99 in grass carp (Ctenopharyngodon idella), suggesting lineage-specific diversification events...
May 2, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28475647/regulation-of-ste20-like-kinase-slk-activity-dimerization-and-activation-segment-phosphorylation
#14
Andrey V Cybulsky, Julie Guillemette, Joan Papillon, Nihad T Abouelazm
The Ste20-like kinase, SLK, has diverse cellular functions. SLK mediates organ development, cell cycle progression, cytoskeletal remodeling, cytokinesis, and cell survival. Expression and activity of SLK are enhanced in renal ischemia-reperfusion injury, and overexpression of SLK was shown to induce apoptosis in cultured glomerular epithelial cells (GECs) and renal tubular cells, as well as GEC/podocyte injury in vivo. The SLK protein consists of a N-terminal catalytic domain and an extensive C-terminal domain, which contains coiled-coils...
2017: PloS One
https://www.readbyqxmd.com/read/28475313/signal-sensing-and-transduction-by-histidine-kinases-as-unveiled-through-studies-on-a-temperature-sensor
#15
Luciano A Abriata, Daniela Albanesi, Matteo Dal Peraro, Diego de Mendoza
Histidine kinases (HK) are the sensory proteins of two-component systems, responsible for a large fraction of bacterial responses to stimuli and environmental changes. Prototypical HKs are membrane-bound proteins that phosphorylate cognate response regulator proteins in the cytoplasm upon signal detection in the membrane or periplasm. HKs stand as potential drug targets but also constitute fascinating systems for studying proteins at work, specifically regarding the chemistry and mechanics of signal detection, transduction through the membrane, and regulation of catalytic outputs...
May 5, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28469162/blue-light-reception-through-quaternary-transitions
#16
Christopher Engelhard, Ralph P Diensthuber, Andreas Möglich, Robert Bittl
Sensory photoreceptors absorb light via their photosensor modules and trigger downstream physiological adaptations via their effector modules. Light reception accordingly depends on precisely orchestrated interactions between these modules, the molecular details of which often remain elusive. Using electron-electron double resonance (ELDOR) spectroscopy and site-directed spin labelling, we chart the structural transitions facilitating blue-light reception in the engineered light-oxygen-voltage (LOV) histidine kinase YF1 which represents a paradigm for numerous natural signal receptors...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28468876/suppressor-analysis-of-the-fusogenic-lambda-spanins
#17
Jesse Cahill, Manoj Rajaure, Ashley Holt, Russell Moreland, Chandler O'Leary, Aneesha Kulkarni, Jordan Sloan, Ry Young
The final step of lysis in λ infections of E. coli is mediated by the spanins Rz and Rz1. These proteins form a complex that bridges the cell envelope and has been proposed to cause fusion of the inner and outer membrane. Accordingly, mutations that block spanin function are found within coiled-coil domains and the proline-rich region, motifs essential in other fusion systems. To gain insight into spanin function, pseudorevertant alleles that restored plaque-formation for lysis-defective mutants of Rz and Rz1 were selected...
May 3, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28467007/fsr1-a-striatin-homolog-forms-an-endomembrane-associated-complex-that-regulates-virulence-in-the-maize-pathogen-fusarium-verticillioides
#18
Huan Zhang, Mala Mukherjee, Jung-Eun Kim, Wenying Yu, Won-Bo Shim
Fsr1, a homolog of mammalian striatin, containing multiple protein-binding domains and a coiled-coil (CC) domain is critical for F. verticillioides virulence. In mammals, striatin interacts with multiple proteins to form a STRIPAK (striatin-interacting phosphatase and kinase) complex that regulates a variety of developmental processes and cellular mechanisms. In this study, we identified the homolog of a key mammalian STRIPAK component STRIP1/2 in F. verticillioides, FvStp1, that interacts with Fsr1 in vivo...
May 3, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28464379/activation-of-transforming-growth-factor-%C3%AE-1-by-thrombin-via-integrins-%C3%AE-v%C3%AE-1-%C3%AE-v%C3%AE-3-and-%C3%AE-v%C3%AE-5-in-buccal-fibroblasts-suppression-by-epigallocatechin-3-gallate
#19
Jenny Zwei-Chieng Chang, Yu-Ping Hsieh, Wen-Hsin Lin, Hsin-Ming Chen, Mark Yen-Ping Kuo
BACKGROUND: Transforming growth factor-beta (TGF-β) plays a central role in the pathogenesis of oral submucous fibrosis (OSF). Thrombin is a key player in tissue repair, inflammation, and fibrosis after injury. METHODS: Effects of thrombin on activated-TGF-β1 levels, Smad3 phosphorylation, and connective tissue growth factor (CTGF/CCN2) synthesis in primary human buccal mucosal fibroblasts (BMFs) were assessed by enzyme-linked immunosorbent assay or Western blot analysis...
May 2, 2017: Head & Neck
https://www.readbyqxmd.com/read/28460455/ccdc106-promotes-non-small-cell-lung-cancer-cell-proliferation
#20
Xiupeng Zhang, Qin Zheng, Chen Wang, Haijing Zhou, Guiyang Jiang, Yuan Miao, Yong Zhang, Yang Liu, Qingchang Li, Xueshan Qiu, Enhua Wang
Coiled-coil domain containing (CCDC) family members enhance tumor cell proliferation, and high CCDC protein levels correlate with unfavorable prognoses. Limited research demonstrated that CCDC106 may promote the degradation of p53/TP53 protein and inhibit its transactivity. The present study demonstrated that CCDC106 expression correlates with advanced TNM stage (P = 0.008), positive regional lymph node metastasis (P < 0.001), and poor overall survival (P < 0.001) in 183 non-small cell lung cancer cases...
April 18, 2017: Oncotarget
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