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Coiled-coil

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https://www.readbyqxmd.com/read/29137403/chicken-ccdc152-shares-an-nfyb-regulated-bidirectional-promoter-with-a-growth-hormone-receptor-antisense-transcript-and-inhibits-cells-proliferation-and-migration
#1
Shudai Lin, Wei Luo, Mingya Jiang, Wen Luo, Bahareldin Ali Abdalla, Qinghua Nie, Li Zhang, Xiquan Zhang
The chicken coiled-coil domain-containing protein 152 (CCDC152) recently has been identified as a novel one implicated in cell cycle regulation, cellular proliferation and migration by us. Here we demonstrate that CCDC152 is oriented in a head-to-head configuration with the antisense transcript of growth hormone receptor (GHR) gene. Through serial luciferase reporter assays, we firstly identified a minimal 102 bp intergenic region as a core bidirectional promoter to drive basal transcription in divergent orientations...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29135996/clinicopathological-and-prognostic-value-of-transforming-acidic-coiled-coil-containing-protein-3-tacc3-expression-in-soft-tissue-sarcomas
#2
Kotaro Matsuda, Hiroaki Miyoshi, Koji Hiraoka, Shintaro Yokoyama, Toshiaki Haraguchi, Toshihiro Hashiguchi, Tetsuya Hamada, Naoto Shiba, Koichi Ohshima
Transforming acidic coiled-coil-containing protein 3 (TACC3), a microtubule regulator, is associated with various cancers. However, the relationship between TACC3 and soft tissue sarcomas (STS) remains unclear. We investigated the expression of TACC3 in 136 STS patient samples using immunohistochemical (IHC) staining, and the statistical associations between TACC3 expression and clinicopathological characteristics were evaluated. Additionally, the expression levels of the tumor suppressor p53 and of the cell proliferation marker Ki-67 were also assessed by IHC...
2017: PloS One
https://www.readbyqxmd.com/read/29133376/distinct-functions-of-starch-synthase-4-domains-in-starch-granule-formation
#3
Kuan-Jen Lu, Barbara Pfister, Camilla Jenny, Simona Eicke, Samuel C Zeeman
The formation of normal starch granules in Arabidopsis leaf chloroplasts requires STARCH SYNTHASE 4 (SS4). In plants lacking SS4, chloroplasts typically produce only one round granule rather than multiple lenticular granules. The mechanisms by which SS4 determines granule number and morphology are not understood. The N-terminal region of SS4 is unique among SS isoforms and contains several long coiled-coil motifs, typically implicated in protein-protein interactions. The C-terminal region contains the catalytic glucosyltransferase domains, which are widely conserved in plant SS and bacterial glycogen synthase (GS) isoforms...
November 13, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29125880/mavs-activates-tbk1-and-ikk%C3%AE%C2%B5-through-trafs-in-nemo-dependent-and-independent-manner
#4
Run Fang, Qifei Jiang, Xiang Zhou, Chenguang Wang, Yukun Guan, Jianli Tao, Jianzhong Xi, Ji-Ming Feng, Zhengfan Jiang
Mitochondrial antiviral-signaling protein (MAVS) transmits signals from RIG-I-like receptors after RNA virus infections. However, the mechanism by which MAVS activates downstream components, such as TBK1 and IKKα/β, is unclear, although previous work suggests the involvement of NEMO or TBK1-binding proteins TANK, NAP1, and SINTBAD. Here, we report that MAVS-mediated innate immune activation is dependent on TRAFs, partially on NEMO, but not on TBK1-binding proteins. MAVS recruited TBK1/IKKε by TRAFs that were pre-associated with TBK1/IKKε via direct interaction between the coiled-coil domain of TRAFs and the SDD domain of TBK1/IKKε...
November 10, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29122888/tnf-stimulated-gene-6-promotes-formation-of-hyaluronan-inter-%C3%AE-inhibitor-heavy-chain-complexes-necessary-for-ozone-induced-airway-hyperresponsiveness
#5
Vandy P Stober, Collin G Johnson, Alana Majors, Mark E Lauer, Valbona Cali, Ronald J Midura, Hans-Georg Wisniewski, Mark A Aronica, Stavros Garantziotis
Exposure to pollutants, such as ozone, exacerbates airway inflammation and hyperresponsiveness (AHR). TNF-stimulated gene 6 (TSG-6) is required to transfer inter-α-inhibitor heavy chains (HC) to hyaluronan (HA), facilitating HA receptors binding. TSG-6 is necessary for AHR in allergic asthma, because it facilitates the development of a pathological HA-HC matrix. However, the role of TSG-6 in acute airway inflammation is not well understood. Here, we hypothesized that TSG-6 is essential for the development of HA- and ozone-induced AHR...
November 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29121029/coiled-coils-unspring-protein-origami
#6
Greg L Hura, John A Tainer
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29118188/structure-and-monomer-dimer-equilibrium-for-the-guanylyl-cyclase-domain-of-the-optogenetics-protein-rhogc
#7
Ramasamy P Kumar, Benjamin R Morehouse, Josiane Fofana, Melissa M Trieu, Daniel H Zhou, Molly O Lorenz, Daniel D Oprian
RhoGC is a fusion protein from the aquatic fungus Blastocladiella emersonii, combining a type I rhodopsin domain with a guanylyl cyclase domain. It has generated excitement as an optogenetics tool for the manipulation of cyclic nucleotide signaling pathways. To investigate the regulation of the cyclase activity, we isolated the guanylyl cyclase domain from Escherichia coli with (GCwCCRho) and without (GCRho) the coiled-coil (CC) linker. Both constructs were constitutively active but were monomeric as determined by size-exclusion chromatography and analytical ultracentrifugation, whereas other class III nucleotidyl cyclases are functional dimers...
November 8, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29115617/three-dimensional-hydrogel-is-suitable-for-targeted-investigation-of-amoeboid-migration-of-glioma-cells
#8
Yubao Huang, Luqing Tong, Li Yi, Chen Zhang, Long Hai, Tao Li, Shengping Yu, Wei Wang, Zhennan Tao, Haiwen Ma, Peidong Liu, Yang Xie, Xuejun Yang
Glioblastoma (GBM) invasion and migration are key biological behaviors leading to refractoriness to current therapies and infiltration into the non‑tumor brain parenchyma. GBM cell migration is strongly dependent on tumor architecture in vivo, which is absent in traditional two‑dimensional (2D) monolayer culture. The present study applied a three‑dimensional (3D) hydrogel model to rebuild the tumor architecture in vitro. Treatment with NSC23766, a specific inhibitor of Ras‑related C3 botulinum toxin substrate 1 (Rac1), inhibited the mesenchymal invasiveness however triggered the amoeboid motility called mesenchymal‑amoeboid transition (MAT)...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115580/overexpression-of-ccdc34-in-colorectal-cancer-and-its-involvement-in-tumor-growth-apoptosis-and-invasion
#9
Wei Geng, Wei Liang, Yanan Fan, Zhibin Ye, Lixiao Zhang
It has been previously reported that increased expression of coiled‑coil domain containing 34 (CCDC34), a member of the CCDCs family, may promote the proliferation and invasion of bladder cancer cells. However, its role in colorectal cancer (CRC) remains unclear. The present study investigated CCDC34 expression in CRC tissues and determined the association between CCDC34 expression and biological characteristics in patients with CRC. Additionally, the variation of cell activity, apoptosis, invasion and associated mechanisms were evaluated following CCDC34 inhibition in SW620 cells with small interfering RNA (siRNA)...
October 24, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115376/ccdc134-serves-a-crucial-role-in-embryonic-development
#10
Biaoyi Yu, Tianzhuo Zhang, Peng Xia, Xiaoting Gong, Xiaoyan Qiu, Jing Huang
Coiled-coil domain containing 134 (CCDC134), a characterized secreted protein, may serve as an immune cytokine and illustrates its potent antitumor effects by augmenting CD8+ T-cell-mediated immunity. Additionally, CCDC134 may also act as a novel regulator of human alteration/deficiency in activation 2a, and be involved in the p300-CBP-associated factor complex and affect its acetyltransferase activity. To clarify the biological and pathological function of CCDC134, the present study generated a viable and fertile Ccdc134fl/fl mouse strain that allowed temporal and spatial control of gene ablation...
October 19, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29112723/in-vitro-and-in-vivo-studies-of-the-als-ftld-protein-chchd10-reveal-novel-mitochondrial-topology-and-protein-interactions
#11
S R Burstein, F Valsecchi, H Kawamata, M Bourens, R Zeng, A Zuberi, T A Milner, S M Cloonan, C Lutz, A Barrientos, G Manfredi
Mutations in coiled-coil-helix-coiled-coil-helix-domain containing 10 (CHCHD10), a mitochondrial twin CX9C protein whose function is still unknown, cause myopathy, motor neuron disease, frontotemporal dementia, and Parkinson's disease. Here, we investigate CHCHD10 topology and its protein interactome, as well as the effects of CHCHD10 depletion or expression of disease-associated mutations in wild-type cells. We find that CHCHD10 associates with membranes in the mitochondrial intermembrane space, where it interacts with a closely related protein, CHCHD2...
November 3, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29111346/evidence-for-m1-linked-polyubiquitin-mediated-conformational-change-in-nemo
#12
Arthur V Hauenstein, Guozhou Xu, Venkataraman Kabaleeswaran, Hao Wu
The NF-κB essential modulator (NEMO) is the scaffolding subunit of the inhibitor of κB kinase (IKK) holocomplex and is required for the activation of the catalytic IKK subunits, IKKα and IKKβ, during the canonical inflammatory response. Although structures of shorter constructs of NEMO have been solved, efforts to elucidate the full-length structure of NEMO have proved difficult due to its apparent high conformational plasticity. To better characterize the gross dimensions of full-length NEMO, we employed in-line size exclusion chromatography-small-angle X-ray scattering...
October 27, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29108261/the-coiled-coil-domain-of-oncogene-rassf-7-inhibits-hippo-signaling-and-promotes-non-small-cell-lung-cancer
#13
Xiaoying Zheng, Qianze Dong, Xiupeng Zhang, Qiang Han, Xu Han, Yong Han, Jingjing Wu, Xuezhu Rong, Enhua Wang
Lung cancer is the leading cause of cancer-related deaths worldwide, and despite recent improvements in treatment patient prognosis remains dismal. In this study, we examined the role of N-terminal Ras-association domain family 7 (RASSF7) in human non-small cell lung cancer (NSCLC). We found that RASSF7 was overexpressed NSCLC tissues, which correlated with advanced TNM stage, positive lymph node metastasis, and poor prognosis. This RASSF7 overexpression promoted lung cancer cell proliferation, migration, and invasion...
October 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29108134/production-of-recombinant-human-tektin-1-2-and-4-and-in-vitro-assembly-of-human-tektin-1
#14
M S Budamagunta, F Guo, N Sun, B Shibata, P G FitzGerald, J V Voss, J F Hess
Proteins predicted to be composed of large stretches of coiled-coil structure have often proven difficult to crystallize for structural determination. We have successfully applied EPR spectroscopic techniques to the study of the structure and assembly of full-length human vimentin assembled into native 11 nm filaments, in physiologic solution, circumventing the limitations of crystallizing shorter peptide sequences. Tektins are a small family of highly alpha helical filamentous proteins found in the doublet microtubules of cilia and related structures...
November 6, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/29107936/a-harpin-elicitor-induces-the-expression-of-a-coiled-coil-nucleotide-binding-leucine-rich-repeat-cc-nb-lrr-defense-signaling-gene-and-others-functioning-during-defense-to-parasitic-nematodes
#15
Weasam A R Aljaafri, Brant T McNeece, Bisho R Lawaju, Keshav Sharma, Prakash M Niruala, Shankar R Pant, David H Long, Kathy S Lawrence, Gary W Lawrence, Vincent P Klink
The bacterial effector harpin induces the transcription of the Arabidopsis thaliana NON-RACE SPECIFIC DISEASE RESISTANCE 1/HARPIN INDUCED1 (NDR1/HIN1) coiled-coil nucleotide binding leucine rich repeat (CC-NB-LRR) defense signaling gene. In Glycine max, Gm-NDR1-1 transcripts have been detected within root cells undergoing a natural resistant reaction to parasitism by the syncytium-forming nematode Heterodera glycines, functioning in the defense response. Expressing Gm-NDR1-1 in Gossypium hirsutum leads to resistance to Meloidogyne incognita parasitism...
October 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29107755/interaction-between-shock-coils-increased-the-incidence-of-inappropriate-therapies-and-lead-failure-in-implantable-cardioverter-defibrillator
#16
Mohammad El Garhy, Marc-Alexander Ohlow, Bernward Lauer
AIMS: Shock coil interaction in patients with multiple implantable cardioverter defibrillator (ICD) leads is occasionally observed. We aimed to evaluate the incidence of shock coil interaction and its clinical relevance. METHODS AND RESULTS: All ICD patients (646 patients) who came to follow up control in our ICD ambulance between January 1, 2011, and December 31, 2011 in the department of cardiology in Bad Berka hospital were retrospectively evaluated in this study...
October 28, 2017: Indian Pacing and Electrophysiology Journal
https://www.readbyqxmd.com/read/29107100/expression-purification-and-characterization-of-the-trim49-protein
#17
Dimitrius Santiago Guimarães, Marcelo Damário Gomes
Autophagy is the process of degradation of intracellular proteins through the lysosome. Members of the tripartite motif (TRIM) proteins have shown to directly recognize autophagic cargo and also to act as a hub for the phagophore nucleation complex. The TRIM proteins are classically characterized by the presence of an amino-terminal RING domain and a B-box domain followed by a coiled coil domain. Although regarded as ubiquitin E3 ligases, this activity has been shown only for a minor set of the 79 human TRIM proteins...
November 4, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29105867/structural-destabilization-of-tropomyosin-induced-by-the-cardiomyopathy-linked-mutation-r21h
#18
Thu Ly, Inna Krieger, Dmitri Tolkatchev, Cheyenna Krone, Timothy Moural, Fadel A Samatey, ChulHee Kang, Alla S Kostyukova
The missense mutation R21H in striated muscle tropomyosin is associated with hypertrophic cardiomyopathy, a genetic cardiac disease and a leading cause of sudden cardiac death in young people. Tropomyosin adopts conformation of a coiled-coil that is critical for regulation of muscle contraction. In this study, we investigated the effects of the R21H mutation on the coiled-coil structure of tropomyosin and its interactions with its binding partners, tropomodulin and leiomodin. Using circular dichroism and isothermal titration calorimetry, we found that the mutation profoundly destabilized the structural integrity of αTM1a1-28 Zip, a chimeric peptide containing the first 28 residues of tropomyosin...
November 6, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29101319/folding-of-a-bacterial-integral-outer-membrane-protein-is-initiated-in-the-periplasm
#19
Rakesh Sikdar, Janine H Peterson, D Eric Anderson, Harris D Bernstein
The Bam complex promotes the insertion of β-barrel proteins into the bacterial outer membrane, but it is unclear whether it threads β-strands into the lipid bilayer in a stepwise fashion or catalyzes the insertion of pre-folded substrates. Here, to distinguish between these two possibilities, we analyze the biogenesis of UpaG, a trimeric autotransporter adhesin (TAA). TAAs consist of three identical subunits that together form a single β-barrel domain and an extracellular coiled-coil ("passenger") domain...
November 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/29099309/the-autophagy-inducing-kinases-ulk1-and-ulk2-regulate-axon-guidance-in-the-developing-mouse-forebrain-via-a-noncanonical-pathway
#20
Bo Wang, Rekha Iyengar, Xiujie Li-Harms, Joung Hyuck Joo, Christopher Wright, Alfonso Lavado, Linda Horner, Mao Yang, Jun-Lin Guan, Sharon Frase, Douglas R Green, Xinwei Cao, Mondira Kundu
Mammalian ULK1 (unc-51 like kinase 1) and ULK2, Caenorhabditis elegans UNC-51, and Drosophila melanogaster Atg1 are serine/threonine kinases that regulate flux through the autophagy pathway in response to various types of cellular stress. C. elegans UNC-51 and D. melanogaster Atg1 also promote axonal growth and defasciculation; disruption of these genes results in defective axon guidance in invertebrates. Although disrupting ULK1/2 function impairs normal neurite outgrowth in vitro, the role of ULK1 and ULK2 in the developing brain remains poorly characterized...
November 3, 2017: Autophagy
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