Vladimir N Malashkevich, Natalya G Dulyaninova, Udupi A Ramagopal, Melissa A Liriano, Kristen M Varney, David Knight, Michael Brenowitz, David J Weber, Steven C Almo, Anne R Bresnick
S100A4, a member of the S100 family of Ca(2+)-binding proteins, regulates carcinoma cell motility via interactions with myosin-IIA. Numerous studies indicate that S100A4 is not simply a marker for metastatic disease, but rather has a direct role in metastatic progression. These observations suggest that S100A4 is an excellent target for therapeutic intervention. Using a unique biosensor-based assay, trifluoperazine (TFP) was identified as an inhibitor that disrupts the S100A4/myosin-IIA interaction. To examine the interaction of S100A4 with TFP, we determined the 2...
May 11, 2010: Proceedings of the National Academy of Sciences of the United States of America