keyword
MENU ▼
Read by QxMD icon Read
search

Flow

keyword
https://www.readbyqxmd.com/read/29228469/simultaneous-determination-of-etonogestrel-and-ethinyl-estradiol-in-human-plasma-by-uplc-ms-ms-and-its-pharmacokinetic-study
#1
Sneha G Nair, Daxesh P Patel, Frank J Gonzalez, Bhargav M Patel, Puran Singhal, Darshan V Chaudhary
A selective, sensitive and rapid ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of etonogestrel (ENG) and ethinyl estradiol (EE) in human plasma. The analytes and their deuterated internal standard, ENG-d7 and EE-d4 were extracted from plasma samples by solid phase extraction on HyperSep™ Retain PEP Cartridges. The chromatographic analysis was performed on ACQUITY UPLC HSS Cyano column, 100 A° (50 mm × 2.1 mm, 1.8 μm) column using gradient mobile phase, acetonitrile and 2...
December 11, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29228451/rg108-induces-the-apoptosis-of-endometrial-cancer-ishikawa-cell-lines-by-inhibiting-the-expression-of-dnmt3b-and-demethylation-of-hmlh1
#2
L Yang, J Hou, X-H Cui, L-N Suo, Y-W Lv
OBJECTIVE: The effects of DNA methyltransferase (DNMT) inhibitor RG108 on the proliferation and apoptosis of endometrial cancer was investigated, and whether its mechanism was related to the inhibition of DNMT3B, thereby affecting the human mutL homolog 1 (hMLH1) methylation status and its expression, was further studied. MATERIALS AND METHODS: Culture of human endometrial cancer Ishikawa cell lines: cells grew adhering to the wall in Roswell Park Memorial Institute-1640 (RPMI-1640) medium (supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamic acid)...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228436/the-effect-of-ventilator-mask-atomization-inhalation-of-ipratropium-bromide-and-budesonide-suspension-liquid-in-the-treatment-of-copd-in-acute-exacerbation-period-on-circulating-levels-of-inflammation-and-prognosis
#3
D-H Jiang, X Wang, L-S Liu, D-D Ji, N Zhang
OBJECTIVE: We investigated the effects of ventilator mask atomization inhalation of ipratropium bromide and budesonide suspension liquid in the treatment of acute exacerbation COPD (AECOPD) on circulating levels of inflammatory factors and prognosis. PATIENTS AND METHODS: A total of 86 cases of patients on ventilator support were randomly divided into control group and observation group with 43 cases each. The control group was administered routine treatment including basic disease treatment, anti-infection, maintenance of a stable internal environment, nutritional support, oxygen inhalation and so on...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228429/peripheral-blood-cd8-t-cell-profiles-in-patients-with-psoriatic-arthritis-a-cross-sectional-case-control-study
#4
E Colombo, G Galleri, G L Erre, C Piras, G Biondi, L Taras, A Zinellu, A A Mangoni, R Manetti, M Montesu, G Passiu
OBJECTIVE: While CD4+ T-cells are traditionally regarded as the main pathogenic T-cell subpopulation in psoriatic arthritis (PsA), the role of circulating CD8+ T-cells remains poorly characterized. We evaluated the differential representation of CD8+ T-cell subpopulations in peripheral blood (PB) of PsA patients. PATIENTS AND METHODS: CD8+IL-17+, CD8+IFNγ+ and CD8+IL-17-IL-22+ T-cells were evaluated by flow-cytometry in 25 consecutive PsA patients, 7 rheumatoid arthritis (RA) patients, 16 patients with psoriasis, and 26 healthy controls (HC)...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228427/mir-155-regulates-lymphoma-cell-proliferation-and-apoptosis-through-targeting-socs3-jak-stat3-signaling-pathway
#5
X-D Li, X-M Li, J-W Gu, X-C Sun
OBJECTIVE: Janus kinase (JAK)- signal transducer and activator of transcription (STAT) signaling pathway participates in regulating cell proliferation, differentiation, and apoptosis, and related to lymphoma. Suppressors of cytokine signaling 3 (SOCS3) is a negative regulator of the JAK-STAT signaling pathway. SOCS3 reduction and miR-155 up-regulation are associated with lymphoma pathogenesis. Bioinformatics analysis showed the complementary binding site between miR-155 and SOCS3. This study aimed to investigate the role of miR-155 in regulating SOCS3/JAK-STAT signaling pathway and affecting diffuse large B cell lymphoma (DLBCL) cell proliferation and apoptosis...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228426/lncrna-hotair-contributes-to-the-tumorigenesis-of-nasopharyngeal-carcinoma-via-up-regulating-fasn
#6
D-D Ma, L-L Yuan, L-Q Lin
OBJECTIVE: The therapeutic options for nasopharyngeal carcinoma (NPC) treatment have been restricted mainly to radiotherapy or chemotherapy, and the clinical treatment effect remains unsatisfactory. The primary purpose of this study was to investigate the molecular mechanisms of NPC and to find effective novel therapeutic targets for NPC. PATIENTS AND METHODS: In order to analyze the expression level of lncRNA HOTAIR and FASN in human NPC clinical tissues or NPC cells, total RNA was extracted with TRIzol and the relative mRNA expression levels were detected by quantitative Real-time PCR...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228425/up-regulation-of-linc00346-inhibits-proliferation-of-non-small-cell-lung-cancer-cells-through-mediating-jak-stat3-signaling-pathway
#7
F Wang, J-G Chen, L-L Wang, Z-Z Yan, S-P Chen, X-G Wang
OBJECTIVE: To investigate the expression levels of LINCRNA00346 in tissues and cells in patients with non-small cell lung cancer (NSCLC) and its biological function. PATIENTS AND METHODS: The relative expression levels of LINC 00346 in 70 cases of tissues and cells in NSCLC patients were detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR). The specific interference sequences of LINC 00346 were designed and the transfection efficiency after 48 h was detected...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228422/microrna-582-5p-suppressed-gastric-cancer-cell-proliferation-via-targeting-akt3
#8
Y Jin, L-P Tao, S-C Yao, Q-K Huang, Z-F Chen, Y-J Sun, S-Q Jin
OBJECTIVE: To dissect the functioning mode of miR-582-5p on gastric cancer cell growth and provide therapeutic targets for gastric cancer. PATIENTS AND METHODS: Relative expression levels of miR-582-5p in human gastric cancer tissue samples and gastric cancer-derived cell lines were measured by using quantitative Real-time PCR. Cell proliferation and viability capacities were assessed by cell counting kit-8 (CCK8) assay and colony formation assay. Cell apoptosis and cell cycle distribution were identified by flow cytometry...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228419/expression-and-clinical-significance-of-long-non-coding-rna-ghet1-in-pancreatic-cancer
#9
H-Y Zhou, H Zhu, X-Y Wu, X-D Chen, Z-G Qiao, X Ling, X-M Yao, J-H Tang
OBJECTIVE: To investigate the expression level and biological function of long non-coding RNA gastric carcinoma high expressed transcript 1 (lncRNA-GHET1) in pancreatic ductal adenocarcinoma (pancreatic cancer for short), to analyze the correlation between the expression of GHET1 and clinicopathological features and to explore the role and clinical significance of GHET1 in the development and progression of pancreatic cancer. PATIENTS AND METHODS: The relative expression of GHET1 in 5 human pancreatic cancer cell lines was detected by quantitative Real-time polymerase chain reaction (qRT-PCR)...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228417/mir-155-affects-renal-carcinoma-cell-proliferation-invasion-and-apoptosis-through-regulating-gsk-3%C3%AE-%C3%AE-catenin-signaling-pathway
#10
R-J Wei, C-H Zhang, W-Z Yang
OBJECTIVE: Glycogen Synthase Kinase-3β (GSK-3β) negatively regulates Wnt/β-catenin signaling pathway through degrading β-catenin protein. It plays an inhibitory role in various tumors, while the influence in the pathogenesis of renal carcinoma has not been elucidated. MicroRNA-155 (MiR-155) was found to be upregulated in renal carcinoma tissue. Bioinformatics analysis revealed the complementary binding site between miR-155 and 3'-UTR of GSK-3β. This study investigated the influence of miR-155 in regulating GSK-3β expression, Wnt/β-catenin signaling pathway activity, and renal carcinoma cell proliferation, invasion, and apoptosis...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228367/how-social-structure-drives-the-population-dynamics-of-the-common-vampire-bat-desmodus-rotundus-phyllostomidae
#11
Maïlis Huguin, Nidia Arechiga-Ceballos, Marguerite Delaval, Amandine Guidez, Isaï Jorge de Castro, Vincent Lacoste, Arielle Salmier, Alvaro Aguilar Setien, Claudia Regina Silva, Anne Lavergne, Benoit de Thoisy
Social systems are major drivers of population structure and gene flow, with important effects on dynamics and dispersal of associated populations of parasites. Among bats, the common vampire bat (Desmodus rotundus) has likely one of the most complex social structure. Using autosomal and mitochondrial markers on vampires from Mexico, French Guiana and North Brazil, from both roosting and foraging areas, we observed an isolation by distance at the wider scale and lower but significant differentiation between closer populations (<50 km)...
December 8, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/29228328/hypervascular-vestibular-schwannomas-clinical-characteristics-angiographical-classification-and-surgical-considerations
#12
Yu Teranishi, Michihiro Kohno, Shigeo Sora, Hiroaki Sato, Osamu Nagata
BACKGROUND: There is a rare type of vestibular schwannoma, scarcely discussed in the literature, known as a hypervascular vestibular schwannoma (HVS). OBJECTIVE: To evaluate its biological characteristics, angiographical classification, surgical outcomes, and the significance for surgical consideration, using a large series of this clinical entity. METHODS: The definition for HVS in this study was the tumor stain from the vertebrobasilar system (VBS) in angiography...
December 7, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29228231/novel-echocardiographic-method-to-assess-left-ventricular-chamber-stiffness-and-elevated-end-diastolic-pressure-based-on-time-velocity-integral-measurements-of-pulmonary-venous-and-transmitral-flows
#13
Kazunori Okada, Sanae Kaga, Rika Abiko, Michito Murayama, Takuma Hioka, Masahiro Nakabachi, Shinobu Yokoyama, Hisao Nishino, Ayako Ichikawa, Ayumu Abe, Mutsumi Nishida, Naoya Asakawa, Shingo Tsujinaga, Taichi Hayashi, Hiroyuki Iwano, Satoshi Yamada, Nobuo Masauzi, Taisei Mikami
Aims: The detection of increased left ventricular (LV) chamber stiffness may play an important role in assessing cardiac patients with potential but not overt heart failure. A non-invasive method to estimate it is not established. We investigated whether the echocardiographic backward/forward flow volume ratio from the left atrium (LA) during atrial contraction reflects the LV chamber stiffness. Methods and results: We studied 62 patients who underwent cardiac catheterization and measured their left ventricular end-diastolic pressure (LVEDP) and pressure increase during atrial contraction (ΔPa) from the LV pressure waveform...
December 8, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/29228136/cd177-neutrophils-suppress-epithelial-cell-tumourigenesis-in-colitis-associated-cancer-and-predict-good-prognosis-in-colorectal-cancer
#14
Guangxi Zhou, Kangsheng Peng, Yang Song, Wenjing Yang, Weigang Shu, Tianming Yu, Lin Yu, Moubin Lin, Qing Wei, Chunqiu Chen, Lu Yin, Yingzi Cong, Zhanju Liu
Neutrophils are found to be infiltrated in tumour tissues of patients with colitis-associated cancer (CAC) and colorectal cancer (CRC), and CD177 is mainly expressed in neutrophils. In our study, expression of CD177 in tumour tissues from patients with CAC or CRC was analysed by qRT-PCR, flow cytometry, and immunohistochemistry. We recruited 378 patients with CRC, determined CD177 expression in tumours, and examined its correlation with clinicopathological features. Moreover, CAC model was induced in wild type (WT) and CD177-/- mice by azoxymethane (AOM)/dextran sodium sulfate (DSS)...
December 8, 2017: Carcinogenesis
https://www.readbyqxmd.com/read/29228043/novel-systems-for-the-application-of-isolated-tensile-compressive-and-shearing-stimulation-of-distraction-callus-tissue
#15
Nicholaus Meyers, Julian Schülke, Anita Ignatius, Lutz Claes
BACKGROUND: Distraction osteogenesis is a procedure widely used for the correction of large bone defects. However, a high complication rate persists, likely due to insufficient stability during maturation. Numerical fracture healing models predict bone regeneration under different mechanical conditions allowing fixation stiffness optimization. However, most models apply a linear elastic material law inappropriate for the transient stresses/strains present during limb lengthening or segment transport...
2017: PloS One
https://www.readbyqxmd.com/read/29228031/transcriptional-profiling-of-pbmcs-unravels-b-cell-mediated-immunopathogenic-imprints-of-hcv-vasculitis
#16
Emily Comstock, Cheol-Woo Kim, Alison Murphy, Benjamin Emmanuel, Xi Zhang, Michael Sneller, Bhawna Poonia, Shyamasundaran Kottilil
B cell depletion therapy using rituximab has been shown to be effective in achieving remission in patients with HCV-mixed cryoglobulinemic (MC) vasculitis. Previously, we have demonstrated abnormalities in peripheral immune cells involving neutrophils, chemotaxis, and innate immune activation among patients with HCV-MC vasculitis when compared to HCV patients without vasculitis. In this study, we evaluated the effect of B cell depletion therapy on transcriptional profiles of peripheral blood mononuclear cells before and after riruximab therapy, in order to unravel the pathogenic mechanism involved in HCV-MC vasculitis induced by abnormal B cell proliferation...
2017: PloS One
https://www.readbyqxmd.com/read/29227980/spectral-domain-optical-coherence-tomography-angiography-for-the-diagnosis-and-evaluation-of-polypoidal-choroidal-vasculopathy
#17
Talisa E de Carlo, Gregg T Kokame, Jessica G Shantha, James C Lai, Raymond Wee
PURPOSE: To compare the diagnostic ability of optical coherence tomography angiography (OCTA) with indocyanine green angiography (ICGA) in polypoidal choroidal vasculopathy (PCV). METHODS: Retrospective review of 47 eyes with PCV imaged with ICGA and OCTA. For each eye, it was determined which imaging modality better delineated the PCV complex. The presence of a branching vascular network (BVN) and polyp(s) were noted. RESULTS: PCV was better visualized with ICGA in 21 eyes (44...
December 11, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/29227904/manipulating-the-structure-and-mechanical-properties-of-thermoplastic-polyurethane-polycaprolactone-hybrid-small-diameter-vascular-scaffolds-fabricated-via-electrospinning-using-an-assembled-rotating-collector
#18
Hao-Yang Mi, Xin Jing, Emily Yu, Xiaofeng Wang, Qian Li, Lih-Sheng Turng
The success of blood vessel transplants with vascular scaffolds (VSs) highly depends on their structure and mechanical properties. The fabrication of small diameter vascular scaffolds (SDVSs) mimicking the properties of native blood vessels has been a challenge. Herein, we propose a facile method to fabricate thermoplastic polyurethane (TPU)/polycaprolactone (PCL) hybrid SDVSs via electrospinning using a modified rotating collector. By varying the ratio between the TPU and the PCL, and changing the electrospinning volume, SDVSs with a wavy configuration and different properties could be obtained...
December 1, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29227791/dynamic-content-exchange-between-liprotides
#19
Henriette S Frislev, Stine C L Jakobsen, Signe A Frank, Daniel E Otzen
Liprotides are complexes composed of partially denatured proteins and fatty acids in which the fatty acids form a micelle-like core surrounded by a shell of proteins. Liprotides, composed of α-lactalbumin (aLA) and oleic acid (OA), are similar in components and cytotoxicity to the original HAMLET protein-fatty acid complex. Liprotides composed of aLA and OA kill tumor cells by transferring the OA component to, and thus destabilizing, the cell membrane. Here we investigate liprotides' dynamics of transfer of contents between themselves and membranes using the hydrophobic fluorescent probe pyrene...
December 5, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/29227732/%C3%AE-8-oxoguanine-dna-glycosylase-overexpression-reduces-oxidative-stress-induced-mitochondrial-dysfunction-and-apoptosis-through-the-jnk-signaling-pathway-in-human-bronchial-epithelial-cells
#20
Ziying Lin, Wenya Xu, Chunyan Li, Yahong Wang, Lawei Yang, Bao'an Zou, Shenglan Gao, Weimin Yao, Zeqing Song, Gang Liu
8-Oxoguanine DNA glycosylase (OGG1) is responsible for repairing 8-oxo-7,8-dihydroguanine (8-oxoG). Our previous study demonstrated that α-OGG1 protects cells from oxidative damage-induced apoptosis and mitochondrial dysfunction in human lung cancer cells. However, the function of β-OGG1 remains to be elucidated. In this study, we demonstrated that overexpressed β-OGG1 has the same role as α-OGG1 in protecting human bronchial epithelial cells from apoptosis and mitochondrial dysfunction. Furthermore, flow cytometry, confocal microscopy, and western blotting showed that the overexpression of β-OGG1 could block oxidant-induced apoptosis in human bronchial epithelial cells...
December 2017: DNA and Cell Biology
keyword
keyword
59471
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"