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https://www.readbyqxmd.com/read/28728386/the-relationship-between-odd-and-branched-chain-fatty-acids-and-microbial-nucleic-acid-bases-in-rumen
#1
Keyuan Liu, Xiaoyan Hao, Yang Li, Guobin Luo, Yonggen Zhang, Hangshu Xin
Objective: This study aims to identify the relationship between odd- and branched-chain fatty acids (OBCFAs) and microbial nucleic acid base in the rumen, and to establish a model to accurately predict microbial protein flow by using OBCFA. Methods: To develop the regression equations, data on the rumen contents of individual cows was obtained from 2 feeding experiments. In the first experiment, 3 rumen-fistulated dry dairy cows arranged in a 3×3 Latin square were fed diets of differing forage to concentration ratios (F:C)...
June 26, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728325/the-usefulness-of-surgical-treatment-in-slow-flow-vascular-malformation-patients
#2
Gyu Bin Kang, Yong Chan Bae, Su Bong Nam, Seong Hwan Bae, Ji Yoon Sung
BACKGROUND: Many difficulties exist in establishing a treatment plan for slow-flow vascular malformation (SFVM). In particular, little research has been conducted on the surgical treatment of SFVMs. Thus, we investigated what proportion of SFVM patients were candidates for surgical treatment in clinical practice and how useful surgical treatment was in those patients. METHODS: This study included 109 SFVM patients who received care at the authors' clinic from 2007 to 2015...
July 2017: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/28728294/-depletion-of-gp73-inhibits-invasion-and-metastasis-of-hepatocellular-carcinoma-cells
#3
L P Liu, J K Chen, Y M Liu, D H Zhang, J Zhang, X L Yang
Objective: To explore the in vitro and in vivo effect of GP73 on the proliferation, invasion and metastasis in hepatocellular carcinoma. Methods: GP73 gene was knocked out using CRISPR/Cas9 gene editing system in H22 and HepG2 cells, and stable knock out strains were constructed. The knockout efficiency was measured by western blot. Colony formation assay was used to detect the effect of GP73 on long-term survival ability. Cells were then highly synchronized in G(1) phase upon treatment with cell synchronization reagents (mimosine), and the percentage of cells in G(2)/M phase at different time points was detected by flow cytometry...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728293/-curcumin-induces-apoptosis-and-protective-autophagy-in-human-gastric-cancer-cells-with-different-degree-of-differentiation
#4
W Li, Y Zhou, J Yang, H H Zhang, S L Zhao, T Zhang, J Huo, P Zheng
Objective: To investigate the effect of curcumin on the apoptosis and autophagy of human gastric cancer cells with different degree of differentiation. Methods: Gastric cancer cell lines BGC-823 and MKN-28 were treated with curcumin at different concentrations. The effect of curcumin on cell proliferation was measured by MTT assay. Apoptosis was assessed by flow cytometry. Autophagy status was analyzed by acridine orange staining. The expression levels of apoptotic and autophagy-related proteins were detected by Western blot...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728292/-detection-and-clinical-significance-of-circulating-tumor-cells-in-osteosarcoma-using-immunofluorescence-combined-with-in-situ-hybridization
#5
H Q Zhang, M H Li, Z Wang, P H Lan, Y J Lu, G J Chen, L Wang
Objective: To investigate the clinical significance of detection of circulating tumor cells (CTCs) in peripheral blood from patients with osteosarcoma (OS) using the iFISH (immunofluorescence and fluorescence in situ hybridization) method. Methods: The live cells recovery rate of immune-magnetic beads was evaluated by live-cell fluorescent tracer technology. The expression of CD45 and CK18 on the cell surface of HOS and HepG2 cells was measured by flow cytometry. And the chromosome aneuploidy was detected by centromeric FISH probe CEP8...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728277/-effects-of-pi3k%C3%AE-rhoa-pathway-on-phagocytosis-defect-of-alveolar-macrophages-in-a-mouse-model-of-chronic-obstructive-pulmonary-disease
#6
S T Hu, Q Xia, X L Zeng, H R Bao, X J Liu
Objective: To investigate effects of Phosphoinositide3-Kinases (PI3Kδ)-Ras homolog gene family member A(RhoA) pathway on phagocytosis deficiency of alveolar macrophages (AMs) in a mouse model of chronic obstructive pulmonary disease (COPD). Methods: Twenty mice were exposed to cigarette smoking to establish the COPD model, with 20 mice as the control group. AMs were isolated from lung tissue by discontinuous density gradient centrifugation and then divided into a healthy control group, a COPD group, a healthy IC87114 group and a COPD IC87114 group...
July 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28728239/-effect-of-hydrogen-rich-saline-on-the-cd4-cd25-foxp3-treg-cells-of-allergic-rhinitis-guinea-pigs-model
#7
F F Xu, S Q Yu, C L Zhao, N Che, L Jin, Q L Wang, R M Ge
Objective: To explore the effect of hydrogen-rich saline on the CD4(+) CD25(+) Foxp3(+) Treg cells in a guinea pig model of allergic rhinitis (AR) and investigate the underling anti-inflammatory mechanism. Methods: Using random number table, eighteen guinea pigs were divided into three groups (control group/AR group/HRS group, n=6 of each group). AR guinea pig model was built with ovalbumin and aluminum. The guinea pigs were injected with hydrogen-rich saline (HRS group) for ten days after sensitation. And control group was injected with equal normal saline at the same time...
July 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28728187/in-vivo-microvascular-pattern-of-solitary-juxtapapillary-capillary-hemangioma-on-oct-angiography
#8
M B Thirumalesh, Astha Jain, Sumeet Agrawal, K Bhujang Shetty
Juxtapapillary capillary hemangioma (JCH) is a vascular hamartoma. Fluorescein fundus angiography aids in diagnosis. The vascular morphology of this tumor has not been documented with optical coherence tomography angiography (OCTA), a new, noninvasive diagnostic modality that provides high-resolution images of vascular pattern based on blood flow in the retina and choroid. Although mainly a clinical diagnosis, OCTA helps in studying the intrinsic vascularity of the tumor and helps in differentiating it from other vascular tumors...
July 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28728177/porcine-collagen-transconjunctival-wound-closure-system-for-microincisional-vitrectomy-surgery
#9
Tiffany A Chen, Peter K Kaiser, Rainer Fasching, Darius M Moshfeghi
BACKGROUND AND OBJECTIVE: Microincisional vitrectomy surgery has improved outcomes for vitrectomies; however, wound closure complications remain an important concern. The authors introduce a technique to improve transconjunctival wound closure in vitrectomy surgery. PATIENTS AND METHODS: The authors evaluated the efficacy of porcine collagen plugs on stopping leakage from sclerotomy test sites in vitro and in vivo and assessed wound site healing with histology samples...
July 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28728084/purification-of-monoclonal-antibodies-entirely-in-flow-through-mode
#10
Tsuyoshi Yamada, Koichi Yamamoto, Takashi Ishihara, Shigeru Ohta
A depth filter is widely used in biopharmaceutical manufacturing process. In this study, we found that buffer exchange to reduce conductivity dramatically improved the removal of impurities in depth filtration. The host cell protein clearance was comparable to that of protein A affinity chromatography, which is generally used as a first capture step in monoclonal antibody purification. In addition, a combination of different depth filters showed enhanced purification. Additional flow-through purification is possible without any sample preparation, and a biopharmaceutical-quality purity level (<100ppm for host cell protein and <5% for high molecular weight species) can be attained...
July 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28728033/deposition-pattern-and-tracer-particle-motion-of-evaporating-multi-component-sessile-droplets
#11
Muhammad Amjad, Yang Yang, Ghulam Raza, Hui Gao, Jun Zhang, Leping Zhou, Xiaoze Du, Dongsheng Wen
The understanding of near-wall motion, evaporation behavior and dry pattern of sessile nanofluid droplets is fundamental to a wide range of applications such as painting, spray drying, thin film coating, fuel injection and inkjet printing. However, a deep insight into the heat transfer, fluid flow, near-wall particle velocity and their effects on the resulting dry patterns is still much needed to take the full advantage of these nano-sized particles in the droplet. This work investigates the effect of direct absorptive silicon/silver (Si/Ag) hybrid nanofluids via two experiments...
July 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28728001/modeling-the-effect-of-temperature-control-curtain-on-the-thermal-structure-in-a-deep-stratified-reservoir
#12
Wei He, Jijian Lian, Ye Yao, Mudan Wu, Chao Ma
Temperature-control curtain (TCC) is an effective facility of selective withdrawal. Previous research has estimated the influence of TCC on the outflow temperature, but its effect on the thermal structure of a reservoir area is unknown, which is crucial to the reservoir ecology. For this purpose, taking the Sanbanxi Reservoir as a case study, a 2-D hydrodynamic and temperature model covering the whole reservoir was built and calibrated to simulate the flow and temperature fields under different TCC scenarios, and the change rules of thermal stability and outflow temperature are obtained...
July 17, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28727888/association-of-mental-health-status-with-perception-of-nasal-function
#13
Erika Strazdins, Yu Feng Nie, Raziqah Ramli, Tom Palesy, Jenna M Christensen, George Nicholas Marcells, Richard John Harvey
Importance: Mental health issues are thought to be overrepresented among patients undergoing rhinoplasty and may be associated with patient presentation prior to surgery. Objective: To assess the association of poor mental health with perception of nasal function. Design, Setting, and Participants: A cross-sectional study of patients presenting for airway assessment was performed from December 1, 2011, to October 31, 2015, at 2 tertiary rhinoplasty centers in Sydney, Australia...
July 20, 2017: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/28727738/propagation-characteristics-of-pulverized-coal-and-gas-two-phase-flow-during-an-outburst
#14
Aitao Zhou, Kai Wang, Lingpeng Fan, Bo Tao
Coal and gas outbursts are dynamic failures that can involve the ejection of thousands tons of pulverized coal, as well as considerable volumes of gas, into a limited working space within a short period. The two-phase flow of gas and pulverized coal that occurs during an outburst can lead to fatalities and destroy underground equipment. This article examines the interaction mechanism between pulverized coal and gas flow. Based on the role of gas expansion energy in the development stage of outbursts, a numerical simulation method is proposed for investigating the propagation characteristics of the two-phase flow...
2017: PloS One
https://www.readbyqxmd.com/read/28727580/increase-in-intracranial-pressure-by-application-of-a-rigid-cervical-collar-a-pilot-study-in-healthy-volunteers
#15
Iscander M Maissan, Rein Ketelaars, Boris Vlottes, Sanne E Hoeks, Dennis den Hartog, Robert J Stolker
OBJECTIVES: Rigid cervical collars are known to increase intracranial pressure (ICP) in severe traumatic brain injury (TBI). Cerebral blood flow might decrease according to the Kellie Monroe doctrine. For this reason, the use of the collar in patients with severe TBI has been abandoned from several trauma protocols in the Netherlands. There is no evidence on the effect of a rigid collar on ICP in patients with mild or moderate TBI or indeed patients with no TBI. As a first step we tested the effect in healthy volunteers with normal ICPs and intact autoregulation of the brain...
July 19, 2017: European Journal of Emergency Medicine: Official Journal of the European Society for Emergency Medicine
https://www.readbyqxmd.com/read/28727488/microsatellites-mapping-for-non-model-species-with-chromosomal-rearrangement-a-case-study-in-the-frog-quasipaa-boulengeri-anura-dicroglossidae
#16
Xiuyun Yuan, Siqi Yuan, Ya Liu, Yun Xia, Xiaomao Zeng
Gene mapping is an important resource for understanding the evolution of genes and cytogenetics. Model species with a known genetic map or genome sequence allow for the selection of genetic markers on a desired chromosome, while it is hard to locate these markers on chromosomes of non-model species without such references. A frog species, Quasipaa boulengeri, shows chromosomal rearrangement polymorphisms, making itself a fascinating model for chromosomal speciation mediated by suppressed recombination. However, no markers have been located on its rearranged chromosomes...
July 20, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28727479/extracellular-chloride-is-required-for-efficient-platelet-aggregation
#17
Kirk A Taylor, Darren G S Wilson, Matthew T Harper, Nicholas Pugh
Anion channels perform a diverse range of functions and have been implicated in ATP release, volume regulation, and phosphatidylserine exposure. Platelets have been shown to express several anion channels but their function is incompletely understood. Due to a paucity of specific pharmacological blockers, we investigated the effect of extracellular chloride substitution on platelet activation using aggregometry and flow cytometry. In the absence of extracellular chloride, we observed a modest reduction of the maximum aggregation response to thrombin or collagen-related peptide...
July 20, 2017: Platelets
https://www.readbyqxmd.com/read/28727438/a-wettability-metric-for-characterization-of-capillary-flow-on-textured-superhydrophilic-surfaces
#18
Taylor P Allred, Justin A Weibel, Suresh V Garimella
Surface wettability is typically characterized by measuring the static contact angle of a sessile droplet placed on the surface. For extremely wetting surfaces on which liquid spontaneously spreads into a thin liquid film, the near-zero static contact angle is not amenable to measurement and does not fully describe the wetting behavior. There are unmet needs in microfluidics, boiling heat transfer enhancement, and anti-fogging applications for a metric to characterize highly wetting (i.e., superhydrophilic) textured surfaces based on their capillary-driven liquid pumping performance, as a supplement to the contact angle for this highly wetting regime...
July 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28727323/indocyanine-green-near-infrared-lymphangiography-for-evaluation-of-effectiveness-of-edema-fluid-flow-under-therapeutic-compression
#19
Marzanna T Zaleska, Waldemar L Olszewski
The commonly used modalities for therapy of limb lymphedema are manual lymphatic drainage, manual devices moving edema fluid and intermittent pneumatic compression (IPC). What seems to be necessary for validation of the effect of the compression procedure is imaging of the mobilized moving edema fluid. Picture of edema fluid flow would allow the therapist to use force adjusted to the tissue volume and stiffness differing in various limb regions as well as identify sites of abundant accumulation of fluid requiring more compression...
July 20, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28727201/gene-expression-stasis-and-plasticity-following-migration-into-a-foreign-environment
#20
Brian K Lohman, William E Stutz, Daniel I Bolnick
Selection against migrants is key to maintaining genetic differences between populations linked by dispersal. However, migrants may mitigate fitness costs by proactively choosing among available habitats, or by phenotypic plasticity. We previously reported that a reciprocal transplant of lake and stream stickleback (Gasterosteus aculeatus) found little support for divergent selection. Here, we revisit that experiment to test whether phenotypic plasticity in gene expression may have helped migrants adjust to unfamiliar habitats...
July 20, 2017: Molecular Ecology
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