keyword
MENU ▼
Read by QxMD icon Read
search

bioengineered

keyword
https://www.readbyqxmd.com/read/29351237/bioengineering-of-rfviia-biopharmaceutical-and-structure-characterization-for-biosimilarity-assessment
#1
Othman Montacir, Houda Montacir, Murat Eravci, Andreas Springer, Stephan Hinderlich, Fereidoun Mahboudi, Amirhossein Saadati, Maria Kristina Parr
Eptacog alfa (NovoSeven®) is a vitamin K-dependent recombinant Factor VIIa produced by genetic engineering from baby hamster kidney (BHK) cells as a single peptide chain of 406 residues. After activation, it consists of a light chain (LC) of 152 amino and a heavy chain (HC) of 254 amino acids. Recombinant FVIIa undergoes many post-translational modifications (PTMs). The first ten glutamic acids of the N-terminal moiety are γ-carboxylated, Asn145 and Asn322 are N-glycosylated, and Ser52 and Ser60 are O-glycosylated...
January 19, 2018: Bioengineering
https://www.readbyqxmd.com/read/29348173/methanobactins-maintaining-copper-homeostasis-in-methanotrophs-and-beyond
#2
Grace E Kenney, Amy C Rosenzweig
Methanobactins (Mbns) are ribosomally produced, post-translationally modified natural products that bind copper with high affinity and specificity. Originally identified in methanotrophic bacteria, which have a high need for copper, operons encoding these compounds have also been found in many non-methanotrophic bacteria. The proteins responsible for Mbn biosynthesis include several novel enzymes. Mbn transport involves export through a multidrug efflux pump and re-internalization via a TonB-dependent transporter...
January 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29346308/tissue-engineering-to-improve-immature-testicular-tissue-and-cell-transplantation-outcomes-one-step-closer-to-fertility-restoration-for-prepubertal-boys-exposed-to-gonadotoxic-treatments
#3
REVIEW
Federico Del Vento, Maxime Vermeulen, Francesca de Michele, Maria Grazia Giudice, Jonathan Poels, Anne des Rieux, Christine Wyns
Despite their important contribution to the cure of both oncological and benign diseases, gonadotoxic therapies present the risk of a severe impairment of fertility. Sperm cryopreservation is not an option to preserve prepubertal boys' reproductive potential, as their seminiferous tubules only contain spermatogonial stem cells (as diploid precursors of spermatozoa). Cryobanking of human immature testicular tissue (ITT) prior to gonadotoxic therapies is an accepted practice. Evaluation of cryopreserved ITT using xenotransplantation in nude mice showed the survival of a limited proportion of spermatogonia and their ability to proliferate and initiate differentiation...
January 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29346031/2016-new-horizons-lecture-beyond-imaging-radiology-of-tomorrow
#4
Hedvig Hricak
This article is based on the New Horizons lecture delivered at the 2016 Radiological Society of North America Annual Meeting. It addresses looming changes for radiology, many of which stem from the disruptive effects of the Fourth Industrial Revolution. This is an emerging era of unprecedented rapid innovation marked by the integration of diverse disciplines and technologies, including data science, machine learning, and artificial intelligence-technologies that narrow the gap between man and machine. Technologic advances and the convergence of life sciences, physical sciences, and bioengineering are creating extraordinary opportunities in diagnostic radiology, image-guided therapy, targeted radionuclide therapy, and radiology informatics, including radiologic image analysis...
January 18, 2018: Radiology
https://www.readbyqxmd.com/read/29345568/surface-bioengineered-gold-nanoparticles-for-biomedical-applications
#5
Zhicong Miao, Zilin Gao, Ruoxia Chen, Xiaoqing Yu, Zhiqiang Su, Gang Wei
The conjugation of gold nanoparticles (AuNPs) with biomolecules could create many outstanding biofunctions for the surface-functionalized nanoparticles and extend their biomedical applications. In this review, we summarize the recent advances in the surface bioengineering of AuNPs with biomolecules, such as DNA, proteins, peptides, and biopolymers, in which the details on the structure, functions, and properties of surface-bioengineered AuNPs are discussed. In addition, the surface-biofunctionalization of AuNPs for biomedical applications like biosensing, bioimaging, drug delivery, and tissue engineering are introduced...
January 16, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29344646/engineered-zinc-finger-transcription-factors-inhibit-the-replication-and-transcription-of-hbv-in%C3%A2-vitro-and-in%C3%A2-vivo
#6
Wei Luo, Junxia Wang, Dengfeng Xu, Huili Bai, Yangli Zhang, Yuhong Zhang, Xiaosong Li
In the present study, an artificial zinc-finger transcription factor eukaryotic expression vector specifically recognizing and binding to the hepatitis B virus (HBV) enhancer (Enh) was constructed, which inhibited the replication and expression of HBV DNA. The HBV EnhI‑specific pcDNA3.1‑artificial transcription factor (ATF) vector was successfully constructed, and then transformed or injected into HepG2.2.15 cells and HBV transgenic mice, respectively. The results demonstrated that the HBV EnhI (1,070‑1,234 bp)‑specific ATF significantly inhibited the replication and transcription of HBV DNA in vivo and in vitro...
January 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29341813/regenerative-medicine-a-vehicle-to-infuse-laboratory-bench-modules-into-an-exercise-physiology-curriculum
#7
Jason M Brown, Breonte S Guy, Dawn X Henderson, C Edward Ebert, Jill Harp, Chad D Markert
Regenerative medicine is a novel discipline that both excites undergraduates and may be used as a vehicle to expose students to scientific concepts and opportunities. The goal of this article is to describe the implementation of a National Science Foundation-funded Targeted Infusion Project in which underrepresented minority undergraduates are exposed to laboratory-bench skills and summer research opportunities that they may not have encountered otherwise. A 3-wk infusion of laboratory-bench and data presentation skills, in the context of a regenerative medicine/bioengineering project, aimed to engage students and expose them to opportunities as summer researchers and teaching assistants...
March 1, 2018: Advances in Physiology Education
https://www.readbyqxmd.com/read/29338625/delivery-of-chemotherapeutics-using-spheres-made-of-bioengineered-spider-silks-derived-from-masp1-and-masp2-proteins
#8
Katarzyna Jastrzebska, Anna Florczak, Kamil Kucharczyk, Yinnan Lin, Qin Wang, Andrzej Mackiewicz, David L Kaplan, Hanna Dams-Kozlowska
AIM: Analysis of the properties and chemotherapeutics delivery potential of spheres made of bioengineered spider silks MS1 and MS2. MATERIALS & METHODS: MS1 and MS2 derived from Nephila clavipes dragline silks - MaSp1 and MaSp2, respectively - formed spheres that were compared in terms of physicochemical properties, cytotoxicity and loading/release of chemotherapeutics. RESULTS: MS2 spheres were more dispersed, smaller, of solid core, of higher beta-sheet structure content, and of opposite (negative) charge than MS1 spheres...
January 17, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29336146/coordination-triggered-hierarchical-folate-zinc-supramolecular-hydrogels-leading-to-printable-biomaterials
#9
Kaerdun Liu, Shihao Zang, Rongrong Xue, Jinghui Yang, Lizhi Wang, Jianbin Huang, Yun Yan
Printable hydrogels desired in bioengineering have extremely high demands on biocompatibility and mechanic strength, which can hardly be achieved in conventional hydrogels made with biopolymers. Here we show that on employment of the strategy of coordination triggered hierarchical self-assembly of naturally occurred small molecule folic acid, supramolecular hydrogels with robust mechanical elastic modulus comparable to synthetic double network (DN) polymer gels can be made at concentrations below 1 %. A sequence of hierarchical steps are involved in the formation of this extraordinary hydrogel: petrin rings on folate form tetramers through hydrogen bonding, tetramers stack into nanofibers by π-π stacking, zinc ions cross-link the nanofibers into larger scale fibrils, and further crosslink the fibril network to gel water...
January 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29334399/chondrocyte-burst-promotes-space-for-mineral-expansion
#10
Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto
Analysis of tissue development from multidisciplinary approaches can result in more integrative biological findings, and can eventually allow the development of more effective bioengineering methods. In this study, we analyzed the initial steps of mineral formation during secondary ossification of mouse femur based on biological and bioengineering approaches. We first found that some chondrocytes burst near the mineralized area. External factors that could trigger chondrocyte burst were then investigated. Chondrocyte burst was shown to be modulated by mechanical and osmotic pressure...
January 15, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29334272/extracellular-vesicle-mimetic-nanovesicles-transport-lncrna-h19-as-competing-endogenous-rna-for-the-treatment-of-diabetic-wounds
#11
Shi-Cong Tao, Bi-Yu Rui, Qi-Yang Wang, Ding Zhou, Yang Zhang, Shang-Chun Guo
Diabetic wounds, one of the most enervating complications of diabetes mellitus, affect millions of people worldwide annually. Vascular insufficiency, caused by hyperglycemia, is one of the primary causes and categories of diabetic impaired wound healing. Recently, long noncoding RNA (LncRNA)-H19, which is significantly decreased in diabetes and may be crucial in triggering angiogenesis, has attracted increasing interest. The possible relationship between the decrease of LncRNA-H19 and the impairment of angiogenesis in diabetes could involve impairment of the insulin-phosphatidylinositol 3-kinase (PI3K)-Akt pathway via the interdiction of LncRNA-H19...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29322878/mechanical-cellular-and-proteomic-properties-of-laryngotracheal-cartilage
#12
Christine M Pauken, Richard Heyes, David G Lott
The larynx sometimes requires repair and reconstruction due to cancer resection, trauma, stenosis, or developmental disruptions. Bioengineering has provided some scaffolding materials and initial attempts at tissue engineering, especially of the trachea, have been made. The critical issues of providing protection, maintaining a patent airway, and controlling swallowing and phonation, require that the regenerated laryngotracheal cartilages must have mechanical and material properties that closely mimic native tissue...
January 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29317499/the-oligomerization-state-of-bacterial-enzyme-i-ei-determines-ei-s-allosteric-stimulation-or-competitive-inhibition-by-%C3%AE-ketoglutarate
#13
Trang Truc Nguyen, Rodolfo Ghirlando, Vincenzo Venditti
The bacterial phosphotransferase system (PTS) is a signal transduction pathway that couples phosphoryl transfer to active sugar transport across the cell membrane. The PTS is initiated by phosphorylation of enzyme I (EI) by phosphoenolpyruvate (PEP). The EI phosphorylation state determines the phosphorylation states of all other PTS components and is thought to play a central role in the regulation of several metabolic pathways and to control the biology of bacterial cells at multiple levels, for example, affecting virulence and biofilm formation...
January 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29317081/hyperaccuracy-three-dimensional-reconstruction-is-able-to-maximize-the-efficacy-of-selective-clamping-during-robot-assisted-partial-nephrectomy-for-complex-renal-masses
#14
Francesco Porpiglia, Cristian Fiori, Enrico Checcucci, Daniele Amparore, Riccardo Bertolo
BACKGROUND: Available technologies could avoid global ischemia for the removal of a renal tumor. OBJECTIVE: To present hyperaccuracy three-dimensional (HA3D) reconstruction during robot-assisted partial nephrectomy (RAPN) and compare its efficacy in sponsoring successful selective clamping of renal arterial branches during RAPN. DESIGN, SETTING, AND PARTICIPANTS: Patients undergoing RAPN (January 2016-July 2017) for renal mass PADUA score ≥10 who underwent abdominal computed tomography scan with angiography...
January 6, 2018: European Urology
https://www.readbyqxmd.com/read/29313028/introduction-to-special-issue-2016-translational-medicine-and-bioengineering-conference
#15
EDITORIAL
Kaushal Rege, Samir Mitragotri
No abstract text is available yet for this article.
June 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29313022/introduction-to-special-issue-bioengineered-therapeutics-the-next-generation-of-drug-development
#16
EDITORIAL
Pankaj Karande
No abstract text is available yet for this article.
March 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29295762/from-nature-to-creation-going-around-in-circles-the-art-of-peptide-cyclization
#17
REVIEW
Rui-Yang Zhang, Parashar Thapa, Michael J Espiritu, Vinay Menon, Jon-Paul Bingham
Cyclic peptides and cyclotides are becoming common identities within the present efforts seen in peptide engineering - as we seek approaches to achieve potent biological activity, pharmacological selectivity, structurally stability and oral bioavailability. Yet this unique family of peptides has faced uncommon hurdles in their discovery, synthesis and bioengineering, retaining to characteristics that truly deviate these from their linear counterparts. In this mini-review we take a board spectrum approach to introduce this novel family of biomolecules and the troubles that they face in their sequence and disulfide connectivity assignment, together highlighting the present combined strategies involved in cyclic peptide/cyclotide synthesis and modification...
December 14, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29295559/recent-advances-in-3d-printing-of-aliphatic-polyesters
#18
REVIEW
Ioana Chiulan, Adriana Nicoleta Frone, Călin Brandabur, Denis Mihaela Panaitescu
3D printing represents a valuable alternative to traditional processing methods, clearly demonstrated by the promising results obtained in the manufacture of various products, such as scaffolds for regenerative medicine, artificial tissues and organs, electronics, components for the automotive industry, art objects and so on. This revolutionary technique showed unique capabilities for fabricating complex structures, with precisely controlled physical characteristics, facile tunable mechanical properties, biological functionality and easily customizable architecture...
December 24, 2017: Bioengineering
https://www.readbyqxmd.com/read/29291239/seizure-forecasting-from-idea-to-reality-outcomes-of-the-my-seizure-gauge-epilepsy-innovation-institute-workshop
#19
Sonya B Dumanis, Jaqueline A French, Christophe Bernard, Gregory A Worrell, Brandy E Fureman
The Epilepsy Innovation Institute (Ei2) is a new research program of the Epilepsy Foundation designed to be an innovation incubator for epilepsy. Ei2 research areas are selected based on community surveys that ask people impacted by epilepsy what they would like researchers to focus on. In their 2016 survey, unpredictability was selected as a top issue regardless of seizure frequency or severity. In response to this need, Ei2 launched the My Seizure Gauge challenge, with the end goal of creating a personalized seizure advisory system device...
November 2017: ENeuro
https://www.readbyqxmd.com/read/29290797/engineered-microenvironments-for-maturation-of-stem-cell-derived-cardiac-myocytes
#20
Rachel R Besser, Matthew Ishahak, Vera Mayo, Daniel Carbonero, Isabella Claure, Ashutosh Agarwal
Through the use of stem cell-derived cardiac myocytes, tissue-engineered human myocardial constructs are poised for modeling normal and diseased physiology of the heart, as well as discovery of novel drugs and therapeutic targets in a human relevant manner. This review highlights the recent bioengineering efforts to recapitulate microenvironmental cues to further the maturation state of newly differentiated cardiac myocytes. These techniques include long-term culture, co-culture, exposure to mechanical stimuli, 3D culture, cell-matrix interactions, and electrical stimulation...
2018: Theranostics
keyword
keyword
59458
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"