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https://www.readbyqxmd.com/read/28088667/cell-laden-hydrogels-for-osteochondral-and-cartilage-tissue-engineering
#1
REVIEW
Jingzhou Yang, Yu Shrike Zhang, Kan Yue, Ali Khademhosseini
: Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue engineered artificial matrices that can replace the damaged regions and promote tissue regeneration. Hydrogels are emerging as a promising class of biomaterials for both soft and hard tissue regeneration. Many critical properties of hydrogels, such as mechanical stiffness, elasticity, water content, bioactivity, and degradation, can be rationally designed and conveniently tuned by proper selection of the material and chemistry...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28081294/xenogeneic-bio-root-prompts-the-constructive-process-characterized-by-macrophage-phenotype-polarization-in-rodents-and-nonhuman-primates
#2
Hui Li, Jingjing Sun, Jie Li, Hefeng Yang, Xiangyou Luo, Jinlong Chen, Li Xie, Fangjun Huo, Tian Zhu, Weihua Guo, Weidong Tian
Tissue or organ regeneration using xenogeneic matrices is a promising approach to address the shortage of donor matrices for allotransplantation. Success of such approach has been demonstrated to correlate with macrophage-mediated fibrotic homeostasis and tissue remodeling. The previous studies have demonstrated that treated dentin matrix (TDM) could be a suitable bioactive substrate for allogeneic tooth root regeneration. This study constructed xenogeneic bioengineered tooth root (bio-root) via a combination of porcine TDM (pTDM) with allogeneic dental follicle cells (DFCs)...
January 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28079296/elasticity-in-macrophage-synthesized-biocrystals
#3
Elizabeth M Horstman, Rahul K Keswani, Benjamin A Frey, Phillip M Rzeczycki, Vernon LaLone, Jeffery A Bertke, Paul J A Kenis, Gus R Rosania
Supramolecular crystalline assembly constitutes a rational approach to bioengineer intracellular structures. Here, biocrystals of clofazimine (CFZ) that form in vivo within macrophages were measured to have marked curvature. Isolated crystals, however, showed reduced curvature suggesting that intracellular forces bend these drug crystals. Consistent with the ability of biocrystals to elastically deform, the inherent crystal structure of the principal molecular component of the biocrystals-the hydrochloride salt of CFZ (CFZ-HCl)-has a corrugated packing along the (001) face and weak dispersive bonding in multiple directions...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28078820/improvement-of-the-in-vivo-cellular-repopulation-of-decellularized-cardiovascular-tissues-by-a-detergent-free-non-proteolytic-actin-disassembling-regimen
#4
Alexander Assmann, Marc Struß, Franziska Schiffer, Friederike Heidelberg, Hiroshi Munakata, Elena V Timchenko, Pavel E Timchenko, Tim Kaufmann, Khon Huynh, Yukiharu Sugimura, Quentin Leidl, Antonio Pinto, Volker R Stoldt, Artur Lichtenberg, Payam Akhyari
Low immunogenicity and high repopulation capacity are crucial determinants for the functional and structural performance of acellular cardiovascular implants. The present study evaluates a detergent-free, non-proteolytic, actin-disassembling regimen (BIO) for decellularization of heart valve and vessel grafts, particularly focusing on their bio-functionality. Rat aortic conduits (rAoC; n = 89) and porcine aortic valve samples (n = 106) are decellularized using detergents (group DET) or the BIO regimen...
January 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28077675/post-hoc-assessment-of-the-immunogenicity-of-bioengineered-factor-viia-demonstrates-the-use-of-preclinical-tools
#5
Kasper Lamberth, Stine Louise Reedtz-Runge, Jonathan Simon, Ksenia Klementyeva, Gouri Shankar Pandey, Søren Berg Padkjær, Véronique Pascal, Ileana R León, Charlotte Nini Gudme, Søren Buus, Zuben E Sauna
Immunogenicity is an important consideration in the licensure of a therapeutic protein because the development of neutralizing anti-drug antibodies (ADAs) can affect both safety and efficacy. Neoantigens introduced by bioengineering of a protein drug are a particular cause for concern. The development of a bioengineered recombinant factor VIIa (rFVIIa) analog was discontinued after phase 3 trials because of the development of ADAs. The unmodified parent molecule (rFVIIa), on the other hand, has been successfully used as a drug for more than two decades with no reports of immunogenicity in congenital hemophilia patients with inhibitors...
January 11, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28077065/structure-alignment-based-classification-of-rna-binding-pockets-reveals-regional-rna-recognition-motifs-on-protein-surfaces
#6
Zhi-Ping Liu, Shutang Liu, Ruitang Chen, Xiaopeng Huang, Ling-Yun Wu
BACKGROUND: Many critical biological processes are strongly related to protein-RNA interactions. Revealing the protein structure motifs for RNA-binding will provide valuable information for deciphering protein-RNA recognition mechanisms and benefit complementary structural design in bioengineering. RNA-binding events often take place at pockets on protein surfaces. The structural classification of local binding pockets determines the major patterns of RNA recognition. RESULTS: In this work, we provide a novel framework for systematically identifying the structure motifs of protein-RNA binding sites in the form of pockets on regional protein surfaces via a structure alignment-based method...
January 11, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28074545/development-and-testing-of-a-database-of-nih-research-funding-of-aapm-members-a-report-from-aapm-working-group-for-the-development-of-a-research-database-wgdrd
#7
Brendan Whelan, Eduardo Moros, Rebecca Fahrig, James Deye, Thomas Yi, Michael Woodward, Paul Keall, Jeff Siewerdsen
PURPOSE: To produce and maintain a database of National Institutes of Health (NIH) funding of American Association of Physicists in Medicine (AAPM) members, to perform a top level analysis of this data, and to make this data (hereafter referred to as the AAPM research database) available for the use of the AAPM and its members. METHODS: NIH funded research dating back to 1985 is available for public download through the NIH exporter website, and AAPM membership information dating back to 2002 was supplied by the AAPM...
January 11, 2017: Medical Physics
https://www.readbyqxmd.com/read/28070774/restoring-fertility-with-cryopreserved-prepubertal-testicular-tissue-perspectives-with-hydrogel-encapsulation-nanotechnology-and-bioengineered-scaffolds
#8
Maxime Vermeulen, Jonathan Poels, Francesca de Michele, Anne des Rieux, Christine Wyns
New and improved oncological therapies are now able to cure more than 80% of cancer-affected children in Europe. However, such treatments are gonadotoxic and result in fertility issues, especially in boys who are not able to provide a sperm sample before starting chemo/radiotherapy because of their prepubertal state. For these boys, cryopreservation of immature testicular tissue (ITT) is the only available option, aiming to preserve spermatogonial stem cells (SSCs). Both slow-freezing and vitrification have been investigated to this end and are now applied in a clinical setting for SSC cryopreservation...
January 9, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28068876/tissue-engineered-model-of-human-osteolytic-bone-tumor
#9
Aranzazu Villasante, Alessandro Marturano, Samuel T Robinson, Zen Liu, X Edward Guo, Gordana Vunjak-Novakovic
Ewing's sarcoma (ES) is a poorly differentiated pediatric tumor of aggressive behavior characterized by propensity to metastasize to bone. Interactions between the tumor and bone cells orchestrate a vicious cycle in which tumor cells induce osteoclast differentiation and activation to cause osteolytic lesions, broken bones, pain and hypercalcemia. The lack of controllable models that can recapitulate osteolysis in ES impedes the development of new therapies and limits our understanding of how tumor cells invade bone...
January 9, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28067628/3d-bioprinting-improving-in-vitro-models-of-metastasis-with-heterogeneous-tumor-microenvironments
#10
REVIEW
Jacob L Albritton, Jordan S Miller
Even with many advances in treatment over the past decades, cancer still remains a leading cause of death worldwide. Despite the recognized relationship between metastasis and increased mortality rate, surprisingly little is known about the exact mechanism of metastatic progression. Currently available in vitro models cannot replicate the three-dimensionality and heterogeneity of the tumor microenvironment sufficiently to recapitulate many of the known characteristics of tumors in vivo Our understanding of metastatic progression would thus be boosted by the development of in vitro models that could more completely capture the salient features of cancer biology...
January 1, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28067095/progress-in-bio-manufacture-of-platelets-for-transfusion
#11
Shen Y Heazlewood, Susan K Nilsson, Kellie Cartledge, Cheang Ly Be, Andrew Vinson, Murat Gel, David N Haylock
Blood transfusion services face an ever-increasing demand for donor platelets to meet clinical needs. Whilst strategies for increasing platelet storage life and improving the efficiency of donor platelet collection are important, in the longer term, platelets generated by bio-manufacturing processes will be required to meet demands. Production of sufficient numbers of in vitro-derived platelets for transfusion represents a significant bioengineering challenge. In this review, we highlight recent progress in this area of research and outline the main technical and biological obstacles that need to be met before this becomes feasible and economic...
January 9, 2017: Platelets
https://www.readbyqxmd.com/read/28066993/developing-a-biomimetic-tooth-bud-model
#12
Elizabeth E Smith, Weibo Zhang, Nathan R Schiele, Ali Khademhosseini, Catherine K Kuo, Pamela C Yelick
A long-term goal is to bioengineer, fully functional, living teeth for regenerative medicine and dentistry applications. Biologically based replacement teeth would avoid insufficiencies of the currently used dental implants. Using natural tooth development as a guide, a model was fabricated using post-natal porcine dental epithelial (pDE), porcine dental mesenchymal (pDM) progenitor cells, and human umbilical vein endothelial cells (HUVEC) encapsulated within gelatin methacrylate (GelMA) hydrogels. Previous publications have shown that post-natal DE and DM cells seeded onto synthetic scaffolds exhibited mineralized tooth crowns composed of dentin and enamel...
January 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28066508/a-molecular-genetic-toolbox-for-yarrowia-lipolytica
#13
Erin L Bredeweg, Kyle R Pomraning, Ziyu Dai, Jens Nielsen, Eduard J Kerkhoven, Scott E Baker
BACKGROUND: Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been made in a variety of backgrounds with varying similarity to a comprehensively sequenced strain. RESULTS: We have developed a set of genetic and molecular tools in order to expand capabilities of Y. lipolytica for both biological research and industrial bioengineering applications...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28063256/dissection-of-the-host-pathogen-interaction-in-human-tuberculosis-using-a-bioengineered-3-dimensional-model
#14
Liku B Tezera, Magdalena K Bielecka, Andrew Chancellor, Michaela T Reichmann, Basim Al Shammari, Patience Brace, Alex Batty, Annie Tocheva, Sanjay Jogai, Ben G Marshall, Marc Tebruegge, Suwan N Jayasinghe, Salah Mansour, Paul T Elkington
Cell biology differs between traditional cell culture and 3-dimensional (3-D) systems, and is modulated by the extracellular matrix. Experimentation in 3-D presents challenges, especially with virulent pathogens. Mycobacterium tuberculosis (Mtb) kills more humans than any other infection and is characterised by a spatially organised immune response and extracellular matrix remodelling. We developed a 3-D system incorporating virulent mycobacteria, primary human blood mononuclear cells and collagen-alginate matrix to dissect the host-pathogen interaction...
January 7, 2017: ELife
https://www.readbyqxmd.com/read/28057509/promoting-bioengineered-tooth-innervation-using-nanostructured-and-hybrid-scaffolds
#15
S Kuchler-Bopp, A Larrea, L Petry, Y Idoux-Gillet, V Sebastian, A Ferrandon, P Schwinté, M Arruebo, N Benkirane-Jessel
: The innervation of teeth mediated by axons originating from the trigeminal ganglia is essential for their function and protection. Immunosuppressive therapy using Cyclosporine A (CsA) was found to accelerate the innervation of transplanted tissues and particularly that of bioengineered teeth. To avoid the CsA side effects, we report in this study the preparation of CsA loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles, their embedding on polycaprolactone (PCL)-based scaffolds and their possible use as templates for the innervation of bioengineered teeth...
January 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28055309/current-strategies-to-generate-mature-human-induced-pluripotent-stem-cells-derived-cholangiocytes-and-future-applications
#16
Eduardo Cervantes-Alvarez, Yang Wang, Alexandra Collin de l'Hortet, Jorge Guzman-Lepe, Jiye Zhu, Kazuki Takeishi
Stem cell research has significantly evolved over the last few years, allowing the differentiation of pluripotent cells into almost any kind of lineage possible. Studies that focus on the liver have considerably taken a leap into this novel technology, and hepatocyte-like cells are being generated that are close to resembling actual hepatocytes both genotypically and phenotypically. The potential of this extends from disease models to bioengineering, and even also innovative therapies for end-stage liver disease...
January 5, 2017: Organogenesis
https://www.readbyqxmd.com/read/28054462/optimization-of-biopharmaceutical-downstream-processes-supported-by-mechanistic-models-and-artificial-neural-networks
#17
Silvia M Pirrung, Luuk A M van der Wielen, Ruud F W C Van Beckhoven, Emile J A X van de Sandt, Michel H M Eppink, Marcel Ottens
Downstream process development is a major area of importance within the field of bioengineering. During the design of such a downstream process, important decisions have to be made regarding the type of unit operations as well as their sequence and their operating conditions. Current computational approaches addressing these issues either show a high level of simplification or struggle with computational speed. Therefore, this article presents a new approach that combines detailed mechanistic models and speed-enhancing artificial neural networks...
January 5, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28054378/microrna-annotation-of-plant-genomes-do-it-right-or-not-at-all
#18
Richard S Taylor, James E Tarver, Alireza Foroozani, Philip C J Donoghue
MicroRNAs are non-coding regulators of gene expression and key factors in development, disease, and targets for bioengineering. Consequently, microRNAs have become essential elements of already burgeoning draft plant genome descriptions where their annotation is often particularly poor, contributing unduly to the corruption of public databases. Using the Citrus sinensis as an example, we highlight and review common failings of miRNAome annotations. Understanding and exploiting the role of miRNAs in plant biology will be stymied unless the research community acts decisively to improve the accuracy of miRNAome annotations...
January 5, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28053158/a-bioengineered-living-cell-construct-activates-an-acute-wound-healing-response-in-venous-leg-ulcers
#19
Rivka C Stone, Olivera Stojadinovic, Ashley M Rosa, Horacio A Ramirez, Evangelos Badiavas, Miroslav Blumenberg, Marjana Tomic-Canic
Chronic nonhealing venous leg ulcers (VLUs) are widespread and debilitating, with high morbidity and associated costs; about $15 billion is spent annually on the care of VLUs in the United States. Despite this, there is a paucity of treatments for VLUs because of the lack of pathophysiologic insight into ulcer development as well as the lack of knowledge regarding biologic actions of existing VLU-targeted therapies. The bioengineered bilayered living cellular construct (BLCC) skin substitute is a U.S. Food and Drug Administration-approved biologic treatment for healing VLUs...
January 4, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28052996/a-novel-bioengineered-3d-human-intestinal-model-for-long-term-infection-of-cryptosporidium-parvum
#20
Maria A DeCicco RePass, Ying Chen, Yinan Lin, Wenda Zhou, David L Kaplan, Honorine D Ward
Cryptosporidium spp. are apicomplexan parasites of global importance that cause human diarrheal disease. In vitro culture models to study this parasite with physiological relevance to in vivo infection remain sub-optimal. Thus, the pathogenesis of cryptosporidiosis remains poorly characterized, and interventions for the disease are limited. In this study, we evaluated the potential of a novel bioengineered 3D human intestinal tissue model (which we developed previously) to support long term infection by C. parvum...
January 4, 2017: Infection and Immunity
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