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https://www.readbyqxmd.com/read/28231171/application-of-hybrid-genetic-algorithm-routine-in-optimizing-food-and-bioengineering-processes
#1
Jaya Shankar Tumuluru, Richard McCulloch
Optimization is a crucial step in the analysis of experimental results. Deterministic methods only converge on local optimums and require exponentially more time as dimensionality increases. Stochastic algorithms are capable of efficiently searching the domain space; however convergence is not guaranteed. This article demonstrates the novelty of the hybrid genetic algorithm (HGA), which combines both stochastic and deterministic routines for improved optimization results. The new hybrid genetic algorithm developed is applied to the Ackley benchmark function as well as case studies in food, biofuel, and biotechnology processes...
November 9, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28227472/social-norms-of-good-design-interdisciplinary-perspectives-from-a-survey-of-engineers-and-clinicians-in-bioengineering
#2
Angela N Johnson, Angela N Johnson, Angela N Johnson
In bioengineering training for new researchers and engineers, a great deal of time is spent discussing what constitutes "good" design. Conceptualization of good design, however, varies widely across interdisciplinary team members, with potential to both foster innovation or lead to unproductive conflict. To explore how groups central to bioengineering teams (physicians/clinicians and engineers/physicists) conceptualize good design, we asked 176 professionals in bioengineering to complete a comprehensive online survey including items designed to assess cognitive and moral foundations (validated MFQ30 tool) and custom items assessing perceptions on good design in three areas (good design characteristics, reputation of design approvers, and perceived design patient/consumer suitability)...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28222326/crossing-kingdoms-using-decellularized-plants-as-perfusable-tissue-engineering-scaffolds
#3
Joshua R Gershlak, Sarah Hernandez, Gianluca Fontana, Luke R Perreault, Katrina J Hansen, Sara A Larson, Bernard Y K Binder, David M Dolivo, Tianhong Yang, Tanja Dominko, Marsha W Rolle, Pamela J Weathers, Fabricio Medina-Bolivar, Carole L Cramer, William L Murphy, Glenn R Gaudette
Despite significant advances in the fabrication of bioengineered scaffolds for tissue engineering, delivery of nutrients in complex engineered human tissues remains a challenge. By taking advantage of the similarities in the vascular structure of plant and animal tissues, we developed decellularized plant tissue as a prevascularized scaffold for tissue engineering applications. Perfusion-based decellularization was modified for different plant species, providing different geometries of scaffolding. After decellularization, plant scaffolds remained patent and able to transport microparticles...
February 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/28221682/biotechnology-and-bioengineering-volume-114-number-4-april-2017
#4
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28220863/engineering-botulinum-neurotoxin-c1-as-a-molecular-vehicle-for-intra-neuronal-drug-delivery
#5
Edwin J Vazquez-Cintron, Phillip H Beske, Luis Tenezaca, Bao Q Tran, Jonathan M Oyler, Elliot J Glotfelty, Christopher A Angeles, Aurelia Syngkon, Jean Mukherjee, Suzanne R Kalb, Philip A Band, Patrick M McNutt, Charles B Shoemaker, Konstantin Ichtchenko
Botulinum neurotoxin (BoNT) binds to and internalizes its light chain into presynaptic compartments with exquisite specificity. While the native toxin is extremely lethal, bioengineering of BoNT has the potential to eliminate toxicity without disrupting neuron-specific targeting, thereby creating a molecular vehicle capable of delivering therapeutic cargo into the neuronal cytosol. Building upon previous work, we have developed an atoxic derivative (ad) of BoNT/C1 through rationally designed amino acid substitutions in the metalloprotease domain of wild type (wt) BoNT/C1...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220862/induction-of-hair-follicle-dermal-papilla-cell-properties-in-human-induced-pluripotent-stem-cell-derived-multipotent-lngfr-thy-1-mesenchymal-cells
#6
Ophelia Veraitch, Yo Mabuchi, Yumi Matsuzaki, Takashi Sasaki, Hironobu Okuno, Aki Tsukashima, Masayuki Amagai, Hideyuki Okano, Manabu Ohyama
The dermal papilla (DP) is a specialised mesenchymal component of the hair follicle (HF) that plays key roles in HF morphogenesis and regeneration. Current technical difficulties in preparing trichogenic human DP cells could be overcome by the use of highly proliferative and plastic human induced pluripotent stem cells (hiPSCs). In this study, hiPSCs were differentiated into induced mesenchymal cells (iMCs) with a bone marrow stromal cell phenotype. A highly proliferative and plastic LNGFR(+)THY-1(+) subset of iMCs was subsequently programmed using retinoic acid and DP cell activating culture medium to acquire DP properties...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28214590/green-engineered-biomolecule-capped-silver-and-copper-nanohybrids-using-prosopis-cineraria-leaf-extract-enhanced-antibacterial-activity-against-microbial-pathogens-of-public-health-relevance-and-cytotoxicity-on-human-breast-cancer-cells-mcf-7
#7
U Jinu, M Gomathi, I Saiqa, N Geetha, G Benelli, P Venkatachalam
This research focused on green engineering and characterization of silver (PcAgNPs) and copper nanoparticles (PcCuNPs) using Prosopis cineraria (Pc) leaf extract prepared by using microwave irradiation. We studied their enhanced antimicrobial activity on human pathogens as well as cytotoxicity on breast cancer cells (MCF-7). Biofabricated silver and copper nanoparticles exhibited UV-Visible absorbance peaks at 420 nm and 575 nm, confirming the bioreduction and stabilization of nanoparticles. Nanoparticles were characterized by FTIR, XRD, FESEM, and EDX analysis...
February 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28198902/microvalve-based-bioprinting-process-bio-inks-and-applications
#8
REVIEW
Wei Long Ng, Jia Min Lee, Wai Yee Yeong, May Win Naing
Bioprinting is an emerging research field that has attracted tremendous attention for various applications; it offers a highly automated, advanced manufacturing platform for the fabrication of complex bioengineered constructs. Different bio-inks comprising multiple types of printable biomaterials and cells are utilized during the bioprinting process to improve the homology to native tissues and/or organs in a highly reproducible manner. This paper, presenting a first-time comprehensive yet succinct review of microvalve-based bioprinting, provides an in-depth analysis and comparison of different drop-on-demand bioprinting systems and highlights the important considerations for microvalve-based bioprinting systems...
February 15, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28192413/evolution-of-a-split-rna-polymerase-as-a-versatile-biosensor-platform
#9
Jinyue Pu, Julia Zinkus-Boltz, Bryan C Dickinson
Biosensors that transduce target chemical and biochemical inputs into genetic outputs are essential for bioengineering and synthetic biology. Current biosensor design strategies are often limited by a low signal-to-noise ratio, the extensive optimization required for each new input, and poor performance in mammalian cells. Here we report the development of a proximity-dependent split RNA polymerase (RNAP) as a general platform for biosensor engineering. After discovering that interactions between fused proteins modulate the assembly of a split T7 RNAP, we optimized the split RNAP components for protein-protein interaction detection by phage-assisted continuous evolution (PACE)...
February 13, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28191781/direct-isolation-and-characterization-of-human-nephron-progenitors
#10
Stefano Da Sacco, Matthew E Thornton, Astgik Petrosyan, Maria Lavarreda-Pearce, Sargis Sedrakyan, Brendan H Grubbs, Roger E De Filippo, Laura Perin
Mature nephrons originate from a small population of uninduced nephrogenic progenitor cells (NPs) within the cap mesenchyme. These cells are characterized by the coexpression of SIX2 and CITED1. Many studies on mouse models as well as on human pluripotent stem cells have advanced our knowledge of NPs, but very little is known about this population in humans, since it is exhausted before birth and strategies for its direct isolation are still limited. Here we report an efficient protocol for direct isolation of human NPs without genetic manipulation or stepwise induction procedures...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191779/development-of-a-three-dimensional-bioengineering-technology-to-generate-lung-tissue-for-personalized-disease-modeling
#11
Dan C Wilkinson, Jackelyn A Alva-Ornelas, Jennifer M S Sucre, Preethi Vijayaraj, Abdo Durra, Wade Richardson, Steven J Jonas, Manash K Paul, Saravanan Karumbayaram, Bruce Dunn, Brigitte N Gomperts
Stem cell technologies, especially patient-specific, induced stem cell pluripotency and directed differentiation, hold great promise for changing the landscape of medical therapies. Proper exploitation of these methods may lead to personalized organ transplants, but to regenerate organs, it is necessary to develop methods for assembling differentiated cells into functional, organ-level tissues. The generation of three-dimensional human tissue models also holds potential for medical advances in disease modeling, as full organ functionality may not be necessary to recapitulate disease pathophysiology...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28186467/effect-of-the-microenvironment-on-mesenchymal-stem-cells-paracrine-signalling-opportunities-to-engineer-the-therapeutic-effect
#12
Gina Diamanta Kusuma, James Carthew, Rebecca Lim, Jessica Ellen Frith
Cues from the extracellular environment, including physical stimuli, are well known to affect mesenchymal stem cells (MSCs) properties in terms of proliferation and differentiation. Many therapeutic strategies are now targeting this knowledge to increase the efficacy of cell therapies, typically employed to repair tissue functions in the event of injury, either by direct engraftment into the target tissue or differentiation into mature tissues. However, it is now envisioned that harnessing the repertoire of factors secreted by MSCs (termed the secretome) may provide an alternate to these cell therapies...
February 10, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28186214/monodisperse-phase-transfer-and-surface-bioengineering-of-metal-nanoparticles-via-a-silk-fibroin-protein-corona
#13
Xiao-Wei Huang, Hong Liang, Zhi Li, Jie Zhou, Xian Chen, Shu-Meng Bai, Huang-Hao Yang
Uniform hydrophobic nanoparticles synthesized in nonpolar solvents possess excellent physio-chemical properties, showing great potential in biomedical applications. However, the presence of hydrophobic ligands on their surfaces limits their use under physiological conditions. Inspired by protein coronas present at the nano-bio interface, here we report a facile and universal method for phase transfer and surface bioengineering of hydrophobic nanoparticles using β-sheet-rich silk fibroin, a FDA-approved natural protein...
February 10, 2017: Nanoscale
https://www.readbyqxmd.com/read/28175497/216%C3%A2-human-fat-derived-mesenchymal-stem-cells-bioengineered-to-secrete-bmp4-are-nononcogenic-suppress-glioma-and-prolong-survival
#14
Sara Ganaha, Rawan Al-Kharboosh, Alejandro Ruiz-Valls, Hugo Guerrero-Cazares, Alfredo Quinones-Hinojosa
No abstract text is available yet for this article.
August 1, 2016: Neurosurgery
https://www.readbyqxmd.com/read/28174307/a-bioengineered-three-dimensional-cell-culture-platform-integrated-with-microfluidics-to-address-antimicrobial-resistance-in-tuberculosis
#15
Magdalena K Bielecka, Liku B Tezera, Robert Zmijan, Francis Drobniewski, Xunli Zhang, Suwan Jayasinghe, Paul Elkington
: Antimicrobial resistance presents one of the most significant threats to human health, with the emergence of totally drug-resistant organisms. We have combined bioengineering, genetically modified bacteria, longitudinal readouts, and fluidics to develop a transformative platform to address the drug development bottleneck, utilizing Mycobacterium tuberculosis as the model organism. We generated microspheres incorporating virulent reporter bacilli, primary human cells, and an extracellular matrix by using bioelectrospray methodology...
February 7, 2017: MBio
https://www.readbyqxmd.com/read/28170182/primary-salivary-human-stem-progenitor-cells-undergo-microenvironment-driven-acinar-like-differentiation-in-hyaluronate-hydrogel-culture
#16
Padma Pradeepa Srinivasan, Vaishali N Patel, Shuang Liu, Daniel A Harrington, Matthew P Hoffman, Xinqiao Jia, Robert L Witt, Mary C Farach-Carson, Swati Pradhan-Bhatt
Radiotherapy for head and neck cancer often has undesirable effects on salivary glands that lead to xerostomia or severe dry mouth, which can increase oral infections. Our goal is to engineer functional, three-dimensional (3D) salivary gland neotissue for autologous implantation to provide permanent relief. An immediate need exists to obtain autologous adult progenitor cells as the use of embryonic and induced pluripotent stem cells potentially pose serious risks such as teratogenicity and immunogenic rejection...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28168380/erratum-to-restoring-fertility-with-cryopreserved-prepubertal-testicular-tissue-perspectives-with-hydrogel-encapsulation-nanotechnology-and-bioengineered-scaffolds
#17
Maxime Vermeulen, Jonathan Poels, Francesca de Michele, Anne des Rieux, Christine Wyns
No abstract text is available yet for this article.
February 6, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28167391/bioengineered-three-dimensional-diseased-intervertebral-disc-model-revealed-inflammatory-crosstalk
#18
Akshay Srivastava, Isma Liza Mohd Isa, Peadar Rooney, Abhay Pandit
Without an appropriate disease model, the understanding of the pathophysiology of intervertebral disc degeneration and inflammation is limited. The lack of understanding limits the potential discovery of therapeutic targets as viable treatment options. Here, we report a versatile method to develop a three-dimensional intervertebral disc (IVD) model to study the response of nucleus pulposus (NP) and annulus fibrosus (AF) cells to inflammatory (IL-1β-induced) stimulation. The cell shape regulated IVD model was engineered by modulating the crosslinking of a self-assembled collagen hydrogel...
January 31, 2017: Biomaterials
https://www.readbyqxmd.com/read/28163179/biomimetic-heparan-sulfate-like-coated-e-ptfe-grafts-reduce-in-graft-neointimal-hyperplasia-in-ovine-carotids
#19
Birgit Wulff, Stefan Stahlhoff, Reinhard Vonthein, Andreas Schmidt, Matthias Sigler, Giovanni B Torsello, Monika Herten
BACKGROUND: Thrombogenicity and neointimal hyperplasia are major causes for synthetic vascular graft failure. Bioactive coatings like heparin have improved patency by reducing thrombogenicity, but neointimal hyperplasia still remains an unsolved problem. Surface coatings with heparan sulfate (HS), the major component of the glycocalix of endothelial cells, have shown reduced platelet and cell adhesion in vitro. The aim of the study was to evaluate the in vivo surface properties of expanded ePTFE vascular grafts with a semisynthetic HS-like coating (SSHS)...
February 2, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28158895/mimicking-exercise-in-three-dimensional-bioengineered-skeletal-muscle-to-investigate-cellular-and-molecular-mechanisms-of-physiological-adaptation
#20
REVIEW
Andreas M Kasper, Daniel C Turner, Neil R W Martin, Adam P Sharples
Bioengineering of skeletal muscle in-vitro in order to produce highly aligned myofibres in relevant three dimensional (3D) matrices have allowed scientists to model the in-vivo skeletal muscle niche. This review discusses essential experimental considerations for developing bioengineered muscle in order to investigate exercise mimicking stimuli. We identify current knowledge in the use of electrical stimulation and co-culture with motor neurons to enhance skeletal muscle maturation and contractile function in bioengineered systems in-vitro...
February 3, 2017: Journal of Cellular Physiology
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