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Inner membrane protein

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https://www.readbyqxmd.com/read/29151065/the-mrna-expression-levels-of-uncoupling-proteins-1-and-2-in-mononuclear-cells-from-patients-with-metabolic-disorders-obesity-and-type-2-diabetes-mellitus
#1
Sona Margaryan, Agata Witkowicz, Anna Partyka, Levon Yepiskoposyan, Gayane Manukyan, Lidia Karabon
INTRODUCTION: Type 2 diabetes mellitus (T2DM) and obesity are metabolic disorders whose major hallmark is insulin resistance. Impaired mitochondrial activity, such as reduced ratio of energy production to respiration, has been implicated in the development of insulin resistance. Uncoupling proteins (UCPs) are proton carriers, expressed in the mitochondrial inner membrane, that uncouple oxygen consumption by the respiratory chain from ATP synthesis. AIM: The aim of the study was to determine transcriptional levels of UCP1 and UCP2 in peripheral blood mononuclear cells (PBMCs) from patients with metabolic disorders: T2DM, obesity and from healthy individuals...
October 19, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/29145636/localization-and-functional-characterization-of-the-p-asn965ser-n965s-abca4-variant-in-mice-reveal-pathogenic-mechanisms-underlying-stargardt-macular-degeneration
#2
Laurie L Molday, Daniel Wahl, Marinko Sarunic, Robert S Molday
ABCA4 is a member of the superfamily of ATP-binding cassette (ABC) proteins that transports N-retinylidene-phosphatidylethanolamine (N-Ret-PE) across outer segment disc membranes thereby facilitating the removal of potentially toxic retinoid compounds from photoreceptor cells. Mutations in the gene encoding ABCA4 are responsible for Stargardt disease (STGD1), an autosomal recessive retinal degenerative disease that causes severe vision loss. To define the molecular basis for STGD1 associated with the p.Asn965Ser (N965S) mutation in the Walker A motif of nucleotide binding domain 1 (NBD1), we generated a p...
November 14, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29138437/photosensitized-ina-labelled-protein-1-phil1-is-novel-component-of-the-inner-membrane-complex-and-is-required-for-plasmodium-parasite-development
#3
Ekta Saini, Mohammad Zeeshan, Declan Brady, Rajan Pandey, Gesine Kaiser, Ludek Koreny, Pradeep Kumar, Vandana Thakur, Shreyansh Tatiya, Nicholas J Katris, Rebecca Stanway Limenitakis, Inderjeet Kaur, Judith L Green, Andrew R Bottrill, David S Guttery, Ross F Waller, Volker Heussler, Anthony A Holder, Asif Mohmmed, Pawan Malhotra, Rita Tewari
Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29138251/mitochondrial-inner-membrane-protease-yme1-degrades-outer-membrane-proteins-tom22-and-om45
#4
Xi Wu, Lanlan Li, Hui Jiang
Mitochondria are double-membraned organelles playing essential metabolic and signaling functions. The mitochondrial proteome is under surveillance by two proteolysis systems: the ubiquitin-proteasome system degrades mitochondrial outer-membrane (MOM) proteins, and the AAA proteases maintain the proteostasis of intramitochondrial compartments. We previously identified a Doa1-Cdc48(-Ufd1-Npl4) complex that retrogradely translocates ubiquitinated MOM proteins to the cytoplasm for degradation. In this study, we report the unexpected identification of MOM proteins whose degradation requires the Yme1(-Mgr1-Mgr3)i-AAA protease complex in mitochondrial inner membrane...
November 14, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29135688/membrane-microdomains-and-the-regulation-of-hdl-biogenesis
#5
Jacques Genest, Adel Schwertani, Hong Y Choi
PURPOSE OF REVIEW: The major cardio-protective function of HDL is to remove excess cellular cholesterol in the process of HDL particle formation and maturation. The HDL biogenic procedure requiring protein-lipid interactions has been incompletely understood, and here we discuss recent progress and insights into the mechanism of HDL biogenesis. RECENT FINDINGS: The initial and rate-limiting step of HDL biogenesis is the interaction between apoA-I and plasma membrane microdomains created by ATP-binding cassette transporter A1 (ABCA1) transporter...
November 11, 2017: Current Opinion in Lipidology
https://www.readbyqxmd.com/read/29126902/identification-and-characterization-of-the-mitochondrial-membrane-sorting-signals-in-phosphatidylserine-decarboxylase-1-from-saccharomyces-cerevisiae
#6
Ariane Wagner, Francesca Di Bartolomeo, Isabella Klein, Claudia Hrastnik, Kim Nguyen Doan, Thomas Becker, Günther Daum
Phosphatidylserine decarboxylase 1 (Psd1p) catalyzes the formation of the majority of phosphatidylethanolamine (PE) in the yeast Saccharomyces cerevisiae. Psd1p is localized to mitochondria, anchored to the inner mitochondrial membrane (IMM) through membrane spanning domains and oriented towards the mitochondrial intermembrane space. We found that Psd1p harbors at least two inner membrane-associated domains, which we named IM1 and IM2. IM1 is important for proper orientation of Psd1p within the IMM (Horvath et al...
November 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29124837/synthetic-ion-channels-and-dna-logic-gates-as-components-of-molecular-robots
#7
Ryuji Kawano
A molecular robot is a next-generation biological robot consisting of biomaterials such as DNA, proteins, and lipids that imitates the actions of microorganisms. Three prerequisites have been proposed for the construction of such a robot: sensor, intelligence, and actuator. This minireview focuses on recent research on synthetic ion channels and DNA computing technologies, which are viewed as potential candidate components of molecular robots. Synthetic ion channels, which are embedded in an artificial cell membrane (lipid bilayer), sense ambient ions or chemicals and incorporate the molecules...
November 9, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29123413/overexpression-of-mpc1-inhibits-the-proliferation-migration-invasion-and-stem-cell-like-properties-of-gastric-cancer-cells
#8
Xiang Zhou, Zhu-Juan Xiong, Shuo-Meng Xiao, Jin Zhou, Zhi Ding, Ling-Chao Tang, Xiao-Dong Chen, Rui Xu, Ping Zhao
Invasion and metastasis are major malignant characteristics of human gastric cancer (GC), but the molecular mechanisms underlying the invasion and metastasis of GC cells remain elusive. MPC1, a key factor that controls pyruvate transportation through the inner mitochondrial membrane, was reported to be downregulated and correlated with poor prognosis in several cancers. However, the effects of MPC1 on human GC have not been illustrated. In this study, we investigated the potential role of MPC1 in the proliferation, migration, invasion, and stem cell-like properties of human GC cells and evaluated its prognostic significance for patients with GC...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29120602/porphyrin-assisted-docking-of-a-thermophage-portal-protein-into-lipid-bilayers-nanopore-engineering-and-characterization
#9
Benjamin Cressiot, Sandra J Greive, Wei Si, Tomas C Pascoa, Mehrnaz Mojtabavi, Maria Chechik, Huw T Jenkins, Xueguang Lu, Ke Zhang, Aleksei Aksimentiev, Alfred A Antson, Meni Wanunu
Nanopore-based sensors for nucleic acid sequencing and single-molecule detection typically employ pore-forming membrane proteins with hydrophobic external surfaces, suitable for insertion into a lipid bilayer. In contrast, hydrophilic pore-containing molecules, such as DNA origami, have been shown to require chemical modification to favor insertion into a lipid environment. In this work, we describe a strategy for inserting polar proteins with an inner pore into lipid membranes, focusing here on a circular 12-subunit assembly of the thermophage G20c portal protein...
November 15, 2017: ACS Nano
https://www.readbyqxmd.com/read/29117682/constant-ph-molecular-dynamics-reveals-how-proton-release-drives-the-conformational-transition-of-a-transmembrane-efflux-pump
#10
Zhi Yue, Wei Chen, Helen I Zgurskaya, Jana Shen
AcrB is the inner-membrane transporter of E. coli AcrAB-TolC tripartite efflux complex, which plays a major role in the intrinsic resistance to clinically important antibiotics. AcrB pumps a wide range of toxic substrates by utilizing the proton gradient between periplasm and cytoplasm. Crystal structures of AcrB revealed three distinct conformational states of the transport cycle, substrate access, binding and extrusion, or loose (L), tight (T) and open (O) states. However, the specific residue(s) responsible for proton binding/release and the mechanism of proton-coupled conformational cycling remain controversial...
November 9, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/29117524/cholesterol-promotes-protein-binding-by-affecting-membrane-electrostatics-and-solvation-properties
#11
Milka Doktorova, Frederick A Heberle, Richard L Kingston, George Khelashvili, Michel A Cuendet, Yi Wen, John Katsaras, Gerald W Feigenson, Volker M Vogt, Robert A Dick
Binding of the retroviral structural protein Gag to the cellular plasma membrane is mediated by the protein's matrix (MA) domain. Prominent among MA-PM interactions is electrostatic attraction between the positively charged MA domain and the negatively charged plasma membrane inner leaflet. Previously, we reported that membrane association of HIV-1 Gag, as well as purified Rous sarcoma virus (RSV) MA and Gag, depends strongly on the presence of acidic lipids and is enhanced by cholesterol (Chol). The mechanism underlying this enhancement was unclear...
November 7, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29113818/cytochrome-c-assisted-escape-of-cardiolipin-from-a-model-mitochondrial-membrane
#12
Aby Thong, Valeria Tsoukanova
Binding of cytochrome c (Cytc) to cardiolipin (CL) in the inner mitochondrial membrane is involved with the onset of apoptosis. In this study, we used CL-containing phospholipid monolayers to mimic the inner mitochondrial membrane. Constant pressure insertion assay was employed to monitor the Cytc-induced expansion of membrane area. Simultaneous epifluorescence microscopy imaging afforded the in-situ visualization of phospholipid demixing and sorting in the membrane. The formation of a CL-rich Ld phase has been observed to prelude the insertion of Cytc...
November 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29111973/otoferlin-acts-as-a-ca-2-sensor-for-vesicle-fusion-and-vesicle-pool-replenishment-at-auditory-hair-cell-ribbon-synapses
#13
Nicolas Antoine Michalski, Juan D Goutman, Sarah Marie Auclair, Jacques Boutet de Monvel, Margot Tertrais, Alice Emptoz, Alexandre Parrin, Sylvie Nouaille, Marc Guillon, Martin Sachse, Danica Ciric, Amel Bahloul, Jean-Pierre Hardelin, Roger Bryan Sutton, Paul Avan, Shyam S Krishnakumar, James E Rothman, Didier Dulon, Saaid Safieddine, Christine Petit
Hearing relies on rapid, temporally precise, and sustained neurotransmitter release at the ribbon synapses of sensory cells, the inner hair cells (IHCs). This process requires otoferlin, a six C2-domain, Ca(2+)-binding transmembrane protein of synaptic vesicles. To decipher the role of otoferlin in the synaptic vesicle cycle, we produced knock-in mice (Otof(Ala515,Ala517/Ala515,Ala517)) with lower Ca(2+)-binding affinity of the C2C domain. The IHC ribbon synapse structure, synaptic Ca(2+) currents, and otoferlin distribution were unaffected in these mutant mice, but auditory brainstem response wave-I amplitude was reduced...
November 7, 2017: ELife
https://www.readbyqxmd.com/read/29110394/methylisothiazolinone-may-induce-cell-death-and-inflammatory-response-through-dna-damage-in-human-liver-epithelium-cells
#14
Eun-Jung Park, Sanghwa Kim, Jaerak Chang
Methylisothiazolinone (MIT) is a powerful biocide and preservative, which is widely used alone or in a 1:3 ratio with methylchloroisothiazolinone (MCIT) under the trade name of Kathons in the manufacture of numerous personal and household products. Considering that Kathons injected intravenously is distributed in the blood and then in the liver, we explored the toxic mechanism of MIT on human liver epithelium cells. At 24 h after exposure, MIT bound to the plasma membrane and the inner wall of vacuoles in the cells, and rupture of the cell membrane and nuclear envelop, autophagosome-like vacuoles formation and mitochondrial damage were observed...
November 7, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29109183/mutation-and-suppressor-analysis-of-the-essential-lps-transport-protein-lpta-reveals-strategies-to-overcome-severe-outer-membrane-permeability-defects-in-escherichia-coli
#15
Federica A Falchi, Elisa A Maccagni, Simone Puccio, Clelia Peano, Cristina De Castro, Angelo Palmigiano, Domenico Garozzo, Alessandra M Martorana, Alessandra Polissi, Gianni Dehò, Paola Sperandeo
In Gram-negative bacteria, lipopolysaccharide (LPS) contributes to the robust permeability barrier of the outer membrane (OM), preventing the entry of toxic molecules such as detergents and antibiotics. LPS is transported from the inner membrane (IM) to the OM by the Lpt multiprotein machinery. Defects in LPS transport compromise LPS assembly at the OM and result in increased antibiotic sensitivity. LptA is a key component of the Lpt machine that interacts with the IM protein LptC and chaperons LPS through the periplasm...
November 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29109154/hierarchical-protein-targeting-and-secretion-is-controlled-by-an-affinity-switch-in-the-type-iii-secretion-system-of-enteropathogenic-escherichia-coli
#16
Athina G Portaliou, Konstantinos C Tsolis, Maria S Loos, Vassileia Balabanidou, Josep Rayo, Alexandra Tsirigotaki, Valerie F Crepin, Gad Frankel, Charalampos G Kalodimos, Spyridoula Karamanou, Anastassios Economou
Type III secretion (T3S), a protein export pathway common to Gram-negative pathogens, comprises a trans-envelope syringe, the injectisome, with a cytoplasm-facing translocase channel. Exported substrates are chaperone-delivered to the translocase, EscV in enteropathogenic Escherichia coli, and cross it in strict hierarchical manner, for example, first "translocators", then "effectors". We dissected T3S substrate targeting and hierarchical switching by reconstituting them in vitro using inverted inner membrane vesicles...
November 6, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29097667/stochastic-palmitoylation-of-accessible-cysteines-in-membrane-proteins-revealed-by-native-mass-spectrometry
#17
Remco N P Rodenburg, Joost Snijder, Michiel van de Waterbeemd, Arie Schouten, Joke Granneman, Albert J R Heck, Piet Gros
Palmitoylation affects membrane partitioning, trafficking and activities of membrane proteins. However, how specificity of palmitoylation and multiple palmitoylations in membrane proteins are determined is not well understood. Here, we profile palmitoylation states of three human claudins, human CD20 and cysteine-engineered prokaryotic KcsA and bacteriorhodopsin by native mass spectrometry. Cysteine scanning of claudin-3, KcsA, and bacteriorhodopsin shows that palmitoylation is independent of a sequence motif...
November 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/29097338/expansion-of-kpc-producing-klebsiella-pneumoniae-with-various-mgrb-mutations-giving-rise-to-colistin-resistance-the-role-of-isl3-on-plasmids
#18
Cesira Giordano, Simona Barnini, Constantinos Tsioutis, Monika A Chlebowicz, Effie V Scoulica, Achilleas Gikas, John W Rossen, Alexander W Friedrich, Erik Bathoorn
BACKGROUND: mcr-1 has been reported as first plasmid gene to confer colistin-resistance. In KPC-producing Klebsiella pneumoniae (KPC-KP) isolates, however, colistin resistance is rapidly emerging through other mechanisms. This is frequently the result of disruption of mgrB gene by insertion sequences, for instance ISL3. The aim of this study is to investigate the expansion of mgrB-mutated KPC-KP. In addition, we identify localization and targets of ISL3 sequences within the core and accessory genome of common KPC-KP lineages...
October 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29097228/involvement-of-ppid-in-sec-dependent-protein-translocation
#19
Michaela Fürst, Yufan Zhou, Jana Merfort, Matthias Müller
The periplasmic space in between the inner and outer membrane of Gram-negative bacteria contains numerous chaperones that are involved in the biogenesis and rescue of extra-cytosolic proteins. In contrast to most of those periplasmic chaperones, PpiD is anchored by an N-terminal transmembrane domain within the inner membrane of Escherichia coli. There it is located in close proximity to the SecY subunit of the SecYEG translocon, which is the primary transporter for secretory and membrane proteins. By site-specific cross-linking we now found the periplasmic domain of PpiD also in close vicinity to the SecG subunit of the Sec translocon and we provide the first direct evidence for a functional cooperation between PpiD and the Sec translocon...
October 30, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29095853/characterization-of-the-functions-and-proteomes-associated-with-membrane-rafts-in-chicken-sperm
#20
Ai Ushiyama, Atsushi Tajima, Naoto Ishikawa, Atsushi Asano
Cellular membranes are heterogeneous, and this has a great impact on cellular function. Despite the central role of membrane functions in multiple cellular processes in sperm, their molecular mechanisms are poorly understood. Membrane rafts are specific membrane domains enriched in cholesterol, ganglioside GM1, and functional proteins, and they are involved in the regulation of a variety of cellular functions. Studies of the functional characterization of membrane rafts in mammalian sperm have demonstrated roles in sperm-egg binding and the acrosomal reaction...
2017: PloS One
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