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https://www.readbyqxmd.com/read/28806058/metalloproteases-of-the-inner-mitochondrial-membrane
#1
Roman Levytskyy, Iryna Bohovych, Oleh Khalimonchuk
The inner mitochondrial membrane (IM) is among most protein-rich cellular compartments. The metastable IM sub-proteome where the concentration of proteins is approaching oversaturation creates a challenging protein folding environment with high probability for protein malfunction or aggregation. Failure to maintain protein homeostasis in such a setting can impair functional integrity of the mitochondria and drive clinical manifestations. The IM is equipped with a series of highly conserved, proteolytic complexes dedicated to the maintenance of normal protein homeostasis within this mitochondrial sub-compartment...
August 14, 2017: Biochemistry
https://www.readbyqxmd.com/read/28803731/purification-and-characterization-of-the-colicin-a-immunity-protein-in-detergent-micelles
#2
Ane Metola, Ana M Bouchet, Marian Alonso-Mariño, Tammo Diercks, Lena Mäler, Félix M Goñi, Ana R Viguera
The immunity proteins against pore-forming colicins represent a family of integral membrane proteins that reside in the inner membrane of producing cells. Cai, the colicin A immunity protein, was characterized here in detergent micelles by circular dichroism (CD), size exclusion chromatography, chemical cross-linking, nuclear magnetic resonance (NMR) spectroscopy, cysteine accessibility, and colicin A binding in detergent micelles. Bile-salt derivatives induced extensive protein polymerization that precluded further investigation...
August 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28803692/a-janus-faced-im30-ring-involved-in-thylakoid-membrane-fusion-is-assembled-from-im30-tetramers
#3
Michael Saur, Raoul Hennig, Phoebe Young, Kristiane Rusitzka, Nadja Hellmann, Jennifer Heidrich, Nina Morgner, Jürgen Markl, Dirk Schneider
Biogenesis and dynamics of thylakoid membranes likely involves membrane fusion events. Membrane attachment of the inner membrane-associated protein of 30 kDa (IM30) affects the structure of the lipid bilayer, finally resulting in membrane fusion. Yet, how IM30 triggers membrane fusion is largely unclear. IM30 monomers pre-assemble into stable tetrameric building blocks, which further align to form oligomeric ring structures, and differently sized IM30 rings bind to membranes. Based on a 3D reconstruction of IM30 rings, we locate the IM30 loop 2 region at the bottom of the ring and show intact membrane binding but missing fusogenic activity of loop 2 mutants...
August 2, 2017: Structure
https://www.readbyqxmd.com/read/28803609/comic-the-hidden-dynamics-of-mitochondrial-inner-compartments
#4
Bongki Cho, Woong Sun
Mitochondria have evolutionarily, functionally and structurally distinct outer- (OMM) and inner-membranes (IMM). Thus, mitochondrial morphology is controlled by independent but coordinated activity of fission and fusion of the OMM and IMM. Constriction and division of the OMM are mediated by endocytosis-like machineries, which include dynamin-related protein 1 with additional cytosolic vesicle scissoring machineries such as actin filament and Dynamin 2. However, structural alteration of the IMM during mitochondrial division has been poorly understood...
August 14, 2017: BMB Reports
https://www.readbyqxmd.com/read/28794267/coi1-is-a-novel-assembly-factor-of-the-yeast-complex-iii-complex-iv-supercomplex
#5
Ravi K Singhal, Christine Kruse, Juliana Heidler, Valentina Strecker, Klaus Zwicker, Lea Düsterwald, Benedikt Westermann, Johannes M Herrmann, Ilka Wittig, Doron Rapaport
The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build in the mitochondrial inner membrane various supramolecular assemblies. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes...
August 9, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28790314/structural-and-functional-insights-into-the-lipopolysaccharide-abc-transporter-lptb2fg
#6
Haohao Dong, Zhengyu Zhang, Xiaodi Tang, Neil G Paterson, Changjiang Dong
The cell surface of most Gram-negative bacteria contains lipopolysaccharide that is essential for their viability and drug resistance. A 134-kDa protein complex LptB2FG is unique among ATP-binding cassette transporters because it extracts lipopolysaccharide from the external leaflet of the inner membrane and propels it along a filament that extends across the periplasm to directly deliver lipopolysaccharide into the external leaflet of the outer membrane. Here we report the crystal structure of the lipopolysaccharide transporter LptB2FG from Klebsiella pneumoniae, in which both LptF and LptG are composed of a β-jellyroll-like periplasmic domain and six α-helical segments in the transmembrane domain...
August 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28789875/in%C3%A2-vitro-expression-of-sec-dependent-pathway-and-type-4b-secretion-system-in-piscirickettsia-salmonis
#7
Marcos Cortés, Patricio Sánchez, Pamela Ruiz, Ronie Haro, Jerson Sáez, Fabián Sánchez, Mauricio Hernández, Cristian Oliver, Alejandro J Yáñez
Piscirickettsia salmonis is an intracellular bacterium and the causative agent of Piscirickettsiosis, a disease responsible for considerable mortalities in the Chilean salmon farming industry. Currently, P. salmonis protein translocation across the membrane and the mechanisms by which virulence factors are delivered to host cells are poorly understood. However, it is known that Gram-negative bacteria possess several mechanisms that transport proteins to the periplasmic and extracellular compartments. The aim of this study was to evaluate the expressional changes of several genes in the P...
August 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28781081/specific-interaction-of-the-human-mitochondrial-uncoupling-protein-1-with-free-long-chain-fatty-acid
#8
Linlin Zhao, Shuqing Wang, Qianli Zhu, Bin Wu, Zhijun Liu, Bo OuYang, James J Chou
The mitochondrial uncoupling protein 1 (UCP1) generates heat by causing proton leak across the mitochondrial inner membrane that requires fatty acid (FA). The mechanism by which UCP1 uses FA to conduct proton remains unsolved, and it is also unclear whether a direct physical interaction between UCP1 and FA exists. Here, we have shown using nuclear magnetic resonance that FA can directly bind UCP1 at a helix-helix interface site composed of residues from the transmembrane helices H1 and H6. According to the paramagnetic relaxation enhancement data and molecular dynamics simulation, the FA acyl chain appears to fit into the groove between H1 and H6 while the FA carboxylate group interacts with the basic residues near the matrix side of UCP1...
July 19, 2017: Structure
https://www.readbyqxmd.com/read/28777375/inhibition-of-bcl-xl-prevents-pro-death-actions-of-%C3%AE-n-bcl-xl-at-the-mitochondrial-inner-membrane-during-glutamate-excitotoxicity
#9
Han-A Park, Pawel Licznerski, Nelli Mnatsakanyan, Yulong Niu, Silvio Sacchetti, Jing Wu, Brian M Polster, Kambiz N Alavian, Elizabeth A Jonas
ABT-737 is a pharmacological inhibitor of the anti-apoptotic activity of B-cell lymphoma-extra large (Bcl-xL) protein; it promotes apoptosis of cancer cells by occupying the BH3-binding pocket. We have shown previously that ABT-737 lowers cell metabolic efficiency by inhibiting ATP synthase activity. However, we also found that ABT-737 protects rodent brain from ischemic injury in vivo by inhibiting formation of the pro-apoptotic, cleaved form of Bcl-xL, ΔN-Bcl-xL. We now report that a high concentration of ABT-737 (1 μM), or a more selective Bcl-xL inhibitor WEHI-539 (5 μM) enhances glutamate-induced neurotoxicity while a low concentration of ABT-737 (10 nM) or WEHI-539 (10 nM) is neuroprotective...
August 4, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28776612/overcoming-t-gondii-infection-and-intracellular-protein-nanocapsules-as-biomaterials-for-ultrasonically-controlled-drug-release
#10
REVIEW
M S Aw, L Paniwnyk
One of the pivotal matters of concern in intracellular drug delivery is the preparation of biomaterials containing drugs that are compatible with the host target. Nanocapsules for oral delivery are found to be suitable candidates for targeting Toxoplasma gondii (T. gondii), a maneuvering and smart protozoic parasite found across Europe and America that causes a subtle but deadly infection. To overcome this disease, there is much potential of integrating protein-based cells into bioinspired nanocompartments such as via biodegradable cross-linked disulfide polyelectrolyte nanoparticles...
August 4, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28771515/the-role-of-core-and-accessory-type-iv-pilus-genes-in-natural-transformation-and-twitching-motility-in-the-bacterium-acinetobacter-baylyi
#11
Colleen G Leong, Rebecca A Bloomfield, Caroline A Boyd, Amber J Dornbusch, Leah Lieber, Flora Liu, Amie Owen, Erin Slay, Kristine M Lang, C Phoebe Lostroh
Here we present an examination of type IV pilus genes associated with competence and twitching in the bacterium Acinetobacter baylyi (strain ADP1, BD413). We used bioinformatics to identify potential competence and twitching genes and their operons. We measured the competence and twitching phenotypes of the bioinformatically-identified genes. These results demonstrate that competence and twitching in A. baylyi both rely upon a core of the same type IV pilus proteins. The core includes the inner membrane assembly platform (PilC), a periplasmic assemblage connecting the inner membrane assembly platform to the secretin (ComM), a secretin (ComQ) and its associated pilotin (PilF) that assists with secretin assembly and localization, both cytoplasmic pilus retraction ATPases (PilU, PilT), and pilins (ComP, ComB, PilX)...
2017: PloS One
https://www.readbyqxmd.com/read/28771474/a-flagellum-specific-chaperone-facilitates-assembly-of-the-core-type-iii-export-apparatus-of-the-bacterial-flagellum
#12
Florian D Fabiani, Thibaud T Renault, Britta Peters, Tobias Dietsche, Eric J C Gálvez, Alina Guse, Karen Freier, Emmanuelle Charpentier, Till Strowig, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner, Michael Hensel, Marc Erhardt
Many bacteria move using a complex, self-assembling nanomachine, the bacterial flagellum. Biosynthesis of the flagellum depends on a flagellar-specific type III secretion system (T3SS), a protein export machine homologous to the export machinery of the virulence-associated injectisome. Six cytoplasmic (FliH/I/J/G/M/N) and seven integral-membrane proteins (FlhA/B FliF/O/P/Q/R) form the flagellar basal body and are involved in the transport of flagellar building blocks across the inner membrane in a proton motive force-dependent manner...
August 2017: PLoS Biology
https://www.readbyqxmd.com/read/28765894/follistatin-like-protein-1-increases-transepithelial-resistance-in-kidney-epithelial-cells-through-akt-signaling
#13
Fei Chen, Qiang Hu, Huihui Huang, Binbin Chen, Yin Xia, Wenjing Liu
Tight junctions are intercellular junctional structures that control paracellular permeability across epithelial cell sheets, and serve as a barrier to the intramembranic diffusion of components between apical and basolateral cell membrane domains. Follistatin‑like protein 1 (FSTL1) has been reported to promote cellular metabolism and survival. FSTL1 has been revealed to be highly expressed in adult kidney tissues, and high FSTL1 levels have been reported in mouse and human serum samples; however, the roles of FSTL1 in the regulation of kidney function remain to be elucidated...
July 31, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28765554/non-peptide-guided-auto-secretion-of-recombinant-proteins-by-super-folder-green-fluorescent-protein-in-escherichia-coli
#14
Zhen Zhang, Rongxing Tang, Dewu Zhu, Wenfeng Wang, Li Yi, Lixin Ma
Protein secretion in Escherichia coli is usually led by a signal peptide that targets the protein to specific secretory pathways. In this study, we demonstrated that the superfolder green fluorescent protein (sfGFP) could be served as a non-signal peptide to guide protein auto-secretion in E. coli. This auto-secretion was characterized as a three-step process through the sub-cellular localization analysis: inner membrane trans-location followed by anchoring at outer membrane, and then being released into culture media...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28765224/genome-wide-sensitivity-analysis-of-the-microsymbiont-sinorhizobium-meliloti-to-symbiotically-important-defensin-like-host-peptides
#15
Markus F F Arnold, Mohammed Shabab, Jon Penterman, Kevin L Boehme, Joel S Griffitts, Graham C Walker
The model legume species Medicago truncatula expresses more than 700 nodule-specific cysteine-rich (NCR) signaling peptides that mediate the differentiation of Sinorhizobium meliloti bacteria into nitrogen-fixing bacteroids. NCR peptides are essential for a successful symbiosis in legume plants of the inverted-repeat-lacking clade (IRLC) and show similarity to mammalian defensins. In addition to signaling functions, many NCR peptides exhibit antimicrobial activity in vitro and in vivo Bacterial resistance to these antimicrobial activities is likely to be important for symbiosis...
August 1, 2017: MBio
https://www.readbyqxmd.com/read/28760865/dystroglycan-maintains-inner-limiting-membrane-integrity-to-coordinate-retinal-development
#16
Reena Clements, Rolf Turk, Kevin P Campbell, Kevin M Wright
Proper neural circuit formation requires the precise regulation of neuronal migration, axon guidance and dendritic arborization. Mutations affecting the function of the transmembrane glycoprotein dystroglycan cause a form of congenital muscular dystrophy that is frequently associated with neurodevelopmental abnormalities. Despite its importance in brain development, the role for dystroglycan in regulating retinal development remains poorly understood. Using a mouse model of dystroglycanopathy (ISPD(L79*) ) and conditional dystroglycan mutants of both sexes, we show that dystroglycan is critical for the proper migration, axon guidance and dendritic stratification of neurons in the inner retina...
July 31, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28760851/a-bacterial-stress-response-regulates-expression-of-respiratory-protein-complexes-to-control-envelope-stress-adaptation
#17
Randi L Guest, Junshu Wang, Julia L Wong, Tracy L Raivio
The Cpx envelope stress response mediates adaptation to stresses that affect protein folding within the envelope of gram-negative bacteria. Recent transcriptome analyses revealed that the Cpx response impacts genes that affect multiple cellular functions predominantly associated with the cytoplasmic membrane. In this study, we examined the connection between the Cpx response and the respiratory complexes NADH dehydrogenase I and cytochrome bo3 in enteropathogenic Escherichia coli We found that the Cpx response directly represses transcription of the nuo and cyo operons and that Cpx-mediated repression of these complexes confers adaptation to stresses that compromise envelope integrity...
July 31, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28760346/a-defect-in-the-mitochondrial-protein-mpv17-underlies-the-transparent-casper-zebrafish
#18
Gianluca D'Agati, Rosanna Beltre, Anna Sessa, Alexa Burger, Yi Zhou, Christian Mosimann, Richard M White
The casper strain of zebrafish is widely used in studies ranging from cancer to neuroscience. casper offers the advantage of relative transparency throughout adulthood, making it particularly useful for in vivo imaging by epifluorescence, confocal, and light sheet microscopy. casper was developed by selective breeding of two previously described recessive pigment mutants: 1) nacre, which harbors an inactivating mutation of the mitfa gene, rendering the fish devoid of pigmented melanocytes; and 2) roy orbison, a mutant with so-far unidentified genetic cause that lacks reflective iridophores...
July 28, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28755372/mass-spectrometry-of-mitochondrial-membrane-protein-complexes
#19
Luc Negroni, Michel Zivy, Claire Lemaire
The ATP production (oxidative phosphorylation) involves five complexes embedded in the inner membrane of mitochondria. The yeast Saccharomyces cerevisiae is mainly used as a model for the study of oxidative phosphorylation; mutants are easy to produce and are still viable due to their ability to grow using the fermentation pathway. Here, we present a process for analyzing mitochondrial respiratory complexes using native electrophoresis (BN-PAGE) coupled to LC-MS/MS. BN-PAGE (1) permits the separation of functional respiratory complexes, thus allowing in-gel activity detection of most of the respiratory complexes and (2) provides convenient samples for bottom-up proteomics...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28753226/golgi-%C3%AE-1-4-fucosyltransferase-of-arabidopsis-thaliana-partially-localizes-at-the-nuclear-envelope
#20
Stephan Rips, Manuel Frank, Annegret Elting, Jan Niklas Offenborn, Antje von Schaewen
We analyzed plant-derived α1,4-Fucosyltransferase (FucTc) homologs by reporter fusions and focused on representatives of the Brassicaceae and Solanaceae. Arabidopsis thaliana AtFucTc-GFP or tomato LeFucTc-GFP restored Lewis-a formation in a fuctc mutant, confirming functionality in the trans-Golgi. AtFucTc-GFP partly accumulated at the nuclear envelope (NE) not observed for other homologs or truncated AtFucTc lacking the N-terminus or catalytic domain. Analysis of At/LeFucTc-GFP swap constructs with exchanged cytosolic, transmembrane and stalk (CTS), or only the CT regions, revealed that sorting information resides in the membrane anchor...
July 28, 2017: Traffic
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