keyword
MENU ▼
Read by QxMD icon Read
search

Infections rrt

keyword
https://www.readbyqxmd.com/read/28742422/virus-isolation-and-molecular-detection-of-bluetongue-and-epizootic-hemorrhagic-disease-viruses-from-naturally-infected-white-tailed-deer-odocoileus-virginianus
#1
Clara Kienzle, Rebecca L Poulson, Mark G Ruder, David E Stallknecht
Hemorrhagic disease in North America is caused by multiple serotypes of epizootic hemorrhagic disease virus (EHDV) and bluetongue virus (BTV). Diagnostic tests for detection of EHDV and BTV include virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and real-time RT-PCR (rRT-PCR). Our objective was to compare the diagnostic capabilities of three rRT-PCR protocols for detection of EHDV and BTV from naturally infected white-tailed deer (Odocoileus virginianus). We compared the effectiveness of these assays to traditional viral detection methods (e...
July 25, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28738865/avian-influenza-h9n2-virus-isolated-from-air-samples-in-lpms-in-jiangxi-china
#2
Xiaoxu Zeng, Mingbin Liu, Heng Zhang, Jingwen Wu, Xiang Zhao, Wenbing Chen, Lei Yang, Fenglan He, Guoyin Fan, Dayan Wang, Haiying Chen, Yuelong Shu
BACKGROUND: Recently, avian influenza virus has caused repeated worldwide outbreaks in humans. Live Poultry Markets (LPMs) play an important role in the circulation and reassortment of novel Avian Influenza Virus (AIVs). Aerosol transmission is one of the most important pathways for influenza virus to spread among poultry, from poultry to mammals, and among mammals. METHODS: In this study, air samples were collected from LPMs in Nanchang city between April 2014 and March 2015 to investigate possible aerosol transmission of AIVs...
July 24, 2017: Virology Journal
https://www.readbyqxmd.com/read/28719301/development-of-a-real-time-reverse-transcription-polymerase-chain-reaction-for-o-nyong-nyong-virus-and-evaluation-with-clinical-and-mosquito-specimens-from-kenya
#3
Jesse Waggoner, Claire Jane Heath, Bryson Ndenga, Francis Mutuku, Malaya K Sahoo, Alisha Mohamed-Hadley, John Vulule, Dunstan Mukoko, A Desiree LaBeaud, Benjamin A Pinsky
O'nyong-nyong virus (ONNV), an alphavirus closely related to chikungunya virus (CHIKV), has been the documented cause of two large outbreaks in east Africa; however, little is known about the contribution of ONNV to cases of acute febrile illness during interepidemic periods. An ONNV real-time reverse transcription polymerase chain reaction (rRT-PCR) was developed and evaluated using clinical and mosquito pool samples. The ONNV rRT-PCR linear range extended from 8.0 to 2.0 log10 copies/μL, and the lower limit of 95% detection was 22...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28717324/investigation-of-avian-influenza-infection-in-wild-birds-in-ismailia-and-damietta-cities-egypt
#4
Hanaa Mohamed Fadel, Rabab Afifi
AIM: This study was carried out to monitor avian influenza (AI) infection in wild birds in Egypt. MATERIALS AND METHODS: A total of 135 wild birds were examined for the presence of H5, H7, and H9 hemagglutination inhibition antibodies. Organs and swab samples of 75 birds were screened by multiplex real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) to detect AI subtypes H5, H7, and H9 matrix genes. RESULTS: The highest seropositive result was recorded in cattle egrets (90...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28695355/suggested-new-breakpoints-of-anti-mers-cov-antibody-elisa-titers-performance-analysis-of-serologic-tests
#5
J-H Ko, M A Müller, H Seok, G E Park, J Y Lee, S Y Cho, Y E Ha, J Y Baek, S H Kim, J-M Kang, Y-J Kim, I J Jo, C R Chung, M-J Hahn, C Drosten, C-I Kang, D R Chung, J-H Song, E-S Kang, K R Peck
To provide optimal cut-off values of anti-Middle East respiratory syndrome coronavirus (MERS-CoV) serologic tests, we evaluated performance of ELISA IgG, ELISA IgA, IFA IgM, and IFA IgG using 138 serum samples of 49 MERS-CoV-infected patients and 219 serum samples of 219 rRT-PCR-negative MERS-CoV-exposed healthcare personnel and patients. The performance analysis was conducted for two different purposes: (1) prediction of neutralization activity in MERS-CoV-infected patients, and (2) epidemiologic surveillance of MERS-CoV infections among MERS-CoV-exposed individuals...
July 11, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28688206/absence-of-clinical-disease-and-contact-transmission-of-north-american-clade-2-3-4-4-h5nx-hpai-in-experimentally-infected-pigs
#6
Bryan S Kaplan, Mia Kim Torchetti, Kelly M Lager, Richard J Webby, Amy L Vincent
BACKGROUND: In the fall of 2014, clade 2.3.4.4 highly pathogenic avian influenza (HPAI) subtype H5N8 was introduced into North America by migrating waterfowl from Asia where, through reassortment, novel HPAI H5N2 and H5N1 viruses emerged. OBJECTIVES: Assess the susceptibility of pigs to North American HPAI clade 2.3.4.4 H5N1, H5N2, and H5N8. METHODS: Pigs and trachea explants were inoculated with a representative panel of clade 2.3.4.4 HPAI viruses from North America...
July 8, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/28662914/development-of-single-step-multiplex-real-time-rt-pcr-assays-for-rapid-diagnosis-of-enterovirus-71-coxsackievirus-a6-and-a16-in-patients-with-hand-foot-and-mouth-disease
#7
Jiratchaya Puenpa, Kamol Suwannakarn, Jira Chansaenroj, Sompong Vongpunsawad, Yong Poovorawan
Real-time reverse-transcription polymerase chain reaction (rRT-PCR) to detect enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) has facilitated the rapid and accurate identification of the two most common etiological agents underlying hand, foot, and mouth disease (HFMD). However, the worldwide emergence of CV-A6 infection in HFMD necessitates development of an improved multiplex rRT-PCR method. To rapidly determine the etiology of HFMD, two rRT-PCR assays using TaqMan probes were developed to differentiate among three selected common enteroviruses (EV-A71, CV-A16 and CV-A6) and to enable broad detection of enteroviruses (pan-enterovirus assay)...
June 27, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28652020/development-of-a-realtime-rt-pcr-assay-for-the-rapid-detection-of-influenza-a-h2-viruses
#8
Komissarov Andrey, Fadeev Artem, Kosheleva Anna, Grudinin Mikhail
Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 1968, however, influenza A(H2) viruses continue to circulate in wild birds and poultry. The lack of immunity in human population and the continued circulation of influenza A(H2) among animals makes emergence of a new pandemic virus possible...
June 23, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28648543/seasonal-influenza-vaccine-effectiveness-against-laboratory-confirmed-influenza-hospitalizations-latin-america-2013
#9
Nathalie El Omeiri, Eduardo Azziz-Baumgartner, Mark G Thompson, Wilfrido Clará, Mauricio Cerpa, Rakhee Palekar, Sara Mirza, Alba María Ropero-Álvarez
BACKGROUND: Despite widespread utilization of influenza vaccines, effectiveness (VE) has not been routinely measured in Latin America. METHODS: We used a case test-negative control design to estimate trivalent inactivated influenza VE against laboratory-confirmed influenza among hospitalized children aged 6months-5years and adults aged ≥60years which are age-groups targeted for vaccination. We sought persons with severe acute respiratory infections (SARI), hospitalized at 71 sentinel hospitals in Argentina, Brazil, Chile, Colombia, Costa Rica, El Salvador, Honduras, Panama, and Paraguay during January-December 2013...
June 22, 2017: Vaccine
https://www.readbyqxmd.com/read/28575115/multiplex-one-step-real-time-pcr-by-taqman-mgb-method-for-rapid-detection-of-pan-and-h5-subtype-avian-influenza-viruses
#10
Zhujun Zhang, Dong Liu, Wenqiang Sun, Jing Liu, Lihong He, Jiao Hu, Min Gu, Xiaoquan Wang, Xiaowen Liu, Shunlin Hu, Sujuan Chen, Daxin Peng, Xiufan Liu
Avian influenza virus (AIV) can infect a variety of avian species and mammals, leading to severe economic losses in poultry industry and posing a substantial threat to public health. Currently, traditional virus isolation and identification is inadequate for the early diagnosis because of its labor-intensive and time-consuming features. Real-time RT-PCR (RRT-PCR) is an ideal method for the detection of AIV since it is highly specific, sensitive and rapid. In addition, as the new quencher MGB is used in RRT-PCR, it only needs shorter probe and helps the binding of target gene and probe...
2017: PloS One
https://www.readbyqxmd.com/read/28500507/impact-of-severe-hematological-abnormalities-in-the-outcome-of-hospitalized-patients-with-influenza-virus-infection
#11
A Lalueza, H Trujillo, J Laureiro, B Ayuso, P Hernández-Jiménez, C Castillo, M Torres, D Folgueira, O Madrid, C Díaz-Pedroche, E Arrieta, C Arévalo, C Lumbreras
Although hematological abnormalities have been described among patients with influenza virus infection, little is known about their impact on the outcome of the patients. The aim of this study was to assess the frequency and clinical impact of severe hematological abnormalities in patients with confirmed influenza virus infection. This was an observational retrospective study including all adult patients with diagnosis of influenza virus infection hospitalized from January to May 2016 in our institution. Influenza virus infection was diagnosed by means of rRT-PCR assay performed on respiratory samples...
May 13, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28491853/infection-associated-acute-interstitial-nephritis-a-case-report
#12
Rupesh Raina, Shirisha Ale, Tushar Chaturvedi, Luke Fraley, Robert Novak, Natthavat Tanphaichitr
BACKGROUND: Acute interstitial nephritis (AIN) is a clinico-pathological syndrome associated with a variety of infections, drugs, and sometimes with unknown causes. It is a common cause of acute kidney injury (AKI) and subsequent renal impairment, which often times is under-diagnosed. Infection-associated AIN occurs as a consequence of many systemic bacterial, viral, and parasitic infec-tions; however, its incidence has decreased significantly after the advent of antimicrobials. Infection-associated AIN presents with both oliguric or non-oliguric renal insufficiency, without the classical clinical triad of AIN (fever, rash, and arthralgia)...
March 2017: Journal of Nephropathology
https://www.readbyqxmd.com/read/28468854/evaluation-of-aptima-zika-virus-assay
#13
Ping Ren, Daniel A Ortiz, Ana C B Terzian, Tatiana E Colombo, Mauricio L Nogueira, Nikos Vasilakis, Michael J Loeffelholz
The Zika virus (ZIKV) epidemic in the Americas poses a public health emergency that requires a swift response. Accurate and reliable ZIKV diagnostic tests serve as an important tool for limiting the spread of ZIKV infections. The Aptima Zika virus assay (Hologic, Marlborough, MA) performed on the automated Panther system is a rapid and high-throughput method for detecting ZIKV RNA using transcription-mediated amplification (TMA) technology. We evaluated the performance characteristics of the Aptima Zika virus assay on clinical serum and urine specimens (n = 124) from two different patient populations and samples spiked with ZIKV from three different lineages (n = 10)...
July 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28455514/enterovirus-d68-subclade-b3-strain-circulating-and-causing-an-outbreak-in-the-united-states-in-2016
#14
Guiqing Wang, Jian Zhuge, Weihua Huang, Sheila M Nolan, Victoria L Gilrane, Changhong Yin, Nevenka Dimitrova, John T Fallon
In 2014 the United States experienced a nationwide outbreak of Enterovirus D68 (EV-D68) infection. There were no confirmed cases of EV-D68 in 2015 and CDC was only aware of limited sporadic EV-D68 detection in the US in 2016. In this report, we analyzed 749 nasopharyngeal (NP) specimens collected in 2015 and 2016 from patients in the Lower Hudson Valley, New York using a previously validated EV-D68-specific rRT-PCR assay. EV-D68 was detected in none of 199 NP specimens collected in 2015, and in one of 108 (0...
April 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28394987/-acute-glomerulonephritis-with-hemodialysis-requirement-secondary-to-influenza-a-h1n1pdm09-infection-report-of-two-cases
#15
Diana Aceituno, Alberto Fica, Rodrigo Fasce, Winston Andrade, Carolina Díaz
Acute renal failure (ARF) requiring hemodialysis is not common among patients affected by influenza. We report two unvaccinated adult patients with smoking habit, which were admitted with severe influenza A H1N1pdm09 that evolved with shock and required mechanical ventilation. Both patients developed progressive renal failure with oliguria/anuria, associated with urinary of inflammatory sediment with proteinuria, microhematuria and in one case also with hypocomplementemia, suggesting acute glomerulonephritis...
February 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28390319/rapid-detection-of-avian-influenza-a-virus-by-immunochromatographic-test-using-a-novel-fluorescent-dye
#16
Seon-Ju Yeo, Bui Thi Cuc, Soon-Ai Kim, Do Thi Hoang Kim, Duong Tuan Bao, Trinh Thi Thuy Tien, Nguyen Thi Viet Anh, Do-Young Choi, Chom-Kyu Chong, Hak Sung Kim, Hyun Park
Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively...
April 2, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28381304/evaluation-of-biosecurity-measures-to-prevent-indirect-transmission-of-porcine-epidemic-diarrhea-virus
#17
Yonghyan Kim, My Yang, Sagar M Goyal, Maxim C-J Cheeran, Montserrat Torremorell
BACKGROUND: The effectiveness of biosecurity methods to mitigate the transmission of porcine epidemic diarrhea virus (PEDV) via farm personnel or contaminated fomites is poorly understood. This study was undertaken to evaluate the effectiveness of biosecurity procedures directed at minimizing transmission via personnel following different biosecurity protocols using a controlled experimental setting. RESULTS: PEDV RNA was detected from rectal swabs of experimentally infected (INF) and sentinel pigs by real-time reverse transcription polymerase chain reaction (rRT-PCR)...
April 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28355409/life-saving-vascular-access-in-vascular-capital-exhaustion-single-center-experience-in-intra-atrial-catheters-for-hemodialysis
#18
Marta Pereira, Noélia Lopez, Iolanda Godinho, Sofia Jorge, Estela Nogueira, Fernando Neves, Alice Fortes, António G Costa
INTRODUCTION: Intra-atrial catheter (IAC) placement through an open surgical approach has emerged as a life-saving technique in hemodialysis (HD) patients with vascular access exhaustion. OBJECTIVE: To assess the complications of IAC placement, as well as patient and vascular access survival after this procedure. METHODS: The authors retrospectively analyzed all seven patients with vascular capital exhaustion, without immediate alternative renal replacement therapy (RRT), who underwent IAC placement between January 2004 and December 2015 at a single center...
March 2017: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/28348777/fatal-case-of-co-infection-with-dengue-virus-and-neisseria-meningitidis-during-a-dengue-epidemic-in-the-state-of-rio-de-janeiro-brazil
#19
Ivano de Filippis, Priscila Conrado Guerra Nunes, Claudia Ferreira de Andrade, Bianca de Santis Gonçalves, Eliane Saraiva de Araújo, Itacirema de Oliveira Bezerra, Ivan Rocha Ferreira da Silva, Rita Maria Nogueira, Ana Maria Bispo de Filippis
INTRODUCTION: Dengue and meningococcal disease are caused by two different agents: a flavivirus and a Gram-negative bacterium, respectively. The first symptoms of both diseases can be indistinct and a rapid and accurate diagnosis is crucial, considering that both diseases are associated with high morbidity and mortality, representing a major public-health problem in Brazil. CASE PRESENTATION: We report a fatal case of co-infection of dengue virus (DENV) and Neisseria meningitidis in a 54-year-old patient...
August 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28342740/the-development-of-a-real-time-reverse-transcription-polymerase-chain-reaction-rrt-pcr-assay-using-taqman-technology-for-the-pan-detection-of-bluetongue-virus-btv
#20
Catherine Mulholland, Michael J McMenamy, Bernd Hoffmann, Bernadette Earley, Bryan Markey, Joseph Cassidy, Gordon Allan, Michael D Welsh, John McKillen
Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time. BTV-8 in particular, caused a major epidemic in northern Europe. As a result, it is evident that most European countries are at risk of BTV infection. The objective of this study was to develop and validate a real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) assay based on TaqMan technology for the detection of representative strains of all BTV serotypes...
March 23, 2017: Journal of Virological Methods
keyword
keyword
59437
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"