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https://www.readbyqxmd.com/read/29150368/the-utility-of-ercp-in-pediatric-pancreatic-trauma
#1
Eric H Rosenfeld, Adam M Vogel, Denise B Klinkner, Mauricio Escobar, Barbara Gaines, Robert Russell, Brendan Campbell, Hale Wills, Anthony Stallion, David Juang, Rajan K Thakkar, Jeffrey Upperman, Mubeen Jafri, Randall Burd, Bindi J Naik-Mathuria
BACKGROUND/PURPOSE: Endoscopic retrograde cholangiopancreatography (ERCP) is an adjunct for pediatric pancreatic injury management, but its use and utility in pediatric patients are unclear. We set out to evaluate the use of ERCP and its effects on outcomes. METHODS: A retrospective review was performed for children who had pancreatic injuries at 22 pediatric trauma centers between 2010 and 2015. ERCP details and outcomes were collected. Analysis was performed using descriptive statistics and Wilcoxon rank-sum tests...
October 13, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29146097/descriptive-study-of-current-therapeutic-practices-clinical-reproductive-findings-and-incidence-of-pregnancy-loss-in-intensively-managed-thoroughbred-mares
#2
B V Rose, M Firth, B Morris, J M Roach, D C Wathes, K L P Verheyen, A M de Mestre
Therapeutic practices in equine reproductive medicine have dramatically evolved over the last 20 years but current usage is not described. The aims of this study were to provide a description of medication use and clinical findings of reproductive examinations alongside measures of reproductive efficiency in thoroughbreds. A prospective cohort study was conducted in the 2013 and 2014 breeding seasons. Mare and stallion details, information on veterinary interventions and findings of reproductive ultrasound scans were collected using questionnaires and entered into a custom-designed Microsoft Access database...
November 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29141579/a-missense-variant-in-the-coil1a-domain-of-the-keratin-25-gene-is-associated-with-the-dominant-curly-hair-coat-trait-crd-in-horse
#3
Caroline Morgenthaler, Mathieu Diribarne, Aurélien Capitan, Rachel Legendre, Romain Saintilan, Maïlys Gilles, Diane Esquerré, Rytis Juras, Anas Khanshour, Laurent Schibler, Gus Cothran
BACKGROUND: Curly horses present a variety of curl phenotypes that are associated with various degrees of curliness of coat, mane, tail and ear hairs. Their origin is still a matter of debate and several genetic hypotheses have been formulated to explain the diversity in phenotype, including the combination of autosomal dominant and recessive alleles. Our purpose was to map the autosomal dominant curly hair locus and identify the causal variant using genome-wide association study (GWAS) and whole-genome sequencing approaches...
November 15, 2017: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/29129877/evaluation-of-the-chemiluminescent-enzyme-immunoassay-system-for-the-measurement-of-testosterone-in-the-serum-and-whole-blood-of-stallions
#4
Yuko Toishi, Nobuo Tsunoda, Shun-Ichi Nagata, Rikio Kirisawa, Kentaro Nagaoka, Gen Watanabe, Yojiro Yanagawa, Seiji Katagiri, Kazuyoshi Taya
Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions...
November 11, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29126628/effect-of-transportation-on-the-sympatho-adrenal-system-responses-in-horses
#5
Pietro Medica, Giuseppe Bruschetta, Cristina Cravana, Adriana Ferlazzo, Esterina Fazio
The objective of current study was to evaluate the effect of transportation stress on the circulating adrenaline (A), noradrenaline (NA) and dopamine (DA) responses of stallions, according to the different distances. Forty-two stallions were studied before and after road transportation of 100, 200 and 300km, for a period of 1-3h. An increase in plasma A after 100km (P<0.001) was observed. A similar result was seen in plasma NA after 100km (P<0.001), and 300km (P<0.001). Increases in plasma DA after 100 and 200km (P<0...
October 25, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29121172/freezing-induced-uptake-of-disaccharides-for-preservation-of-chromatin-in-freeze-dried-stallion-sperm-during-accelerated-aging
#6
Harriëtte Oldenhof, Miao Zhang, Katharina Narten, Judith Bigalk, Bulat Sydykov, Willem F Wolkers, Harald Sieme
Non-viable freeze-dried sperm have intact chromatin and can be used for fertilization via intracytoplasmic sperm injection. Freeze-dried sperm preferably should be stored at 4°C or lower, because DNA damage accumulates during storage at room temperature. Disaccharides are known to protect biomolecules both during freezing and drying, by forming a highly viscous glassy state. Their use for intracellular protection is challenging because cellular membranes are normally impermeable for disaccharides. In the current study, we demonstrate that membrane impermeable compounds, including lucifer yellow and trehalose, are taken up by stallion sperm when exposed to freezing...
November 7, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29115996/serological-evidence-of-equine-arteritis-virus-infection-and-phylogenetic-analysis-of-viral-isolates-in-semen-of-stallions-from-serbia
#7
Sava Lazić, Diana Lupulović, Delphine Gaudaire, Tamas Petrovic, Gospava Lazić, Aymeric Hans
BACKGROUND: Equine arteritis virus (EAV) is responsible for infections in equids. It can spread easily within the horse population and has a major impact on the horse breeding industry. No EAV outbreak has ever been reported in Serbia. To determine whether EAV is nonetheless circulating there, especially in the Vojvodina region, 340 horse serum samples were subjected to serology testing to detect EAV antibodies. In parallel, semen samples from three seropositive stallions were collected to evaluate their EAV status, using RT-qPCR and virus isolation on cell culture...
November 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29112778/clinical-application-of-hysteroscopic-hydrotubation-for-unexplained-infertility-in-the-mare
#8
Y Inoue, M Sekiguchi
BACKGROUND: Therapeutic techniques for oviductal obstruction in the mare are limited. Non-surgical and retrograde flushing may be an attractive alternative to current treatment methods for oviductal blockage. OBJECTIVES: To evaluate hysteroscopic selective hydrotubation as a treatment option for presumptive equine oviductal blockage. STUDY DESIGN: Retrospective case series. METHODS: Ten ml of saline was flushed through the oviducts in 28 standing sedated mares, which had reproductive histories of unexplained subfertility, by inserting a catheter into the uterotubal junction under endoscopic guidance...
November 7, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29052325/first-evidence-for-the-presence-of-aquaporins-in-stallion-sperm
#9
S Bonilla-Correal, F Noto, E Garcia-Bonavila, J E Rodríguez-Gil, M Yeste, J Miro
Aquaporins (AQPs) are transmembrane proteins found in all cells and are responsible for the transport of water and small solutes. While these proteins have been found in the spermatozoa of humans, rodents, pigs and cattle, where not only do they play a role for the regulation of sperm volume but are also related with the sperm resilience to withstand freeze-thawing procedures, their presence in stallion sperm is yet to be reported. Therefore, the objectives of this work were as follows: (i) to determine whether AQP3, AQP7 and AQP11 are present in stallion sperm and (ii) to investigate whether the relative amounts of these three AQPs play any role in the cryopreservation success...
October 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29047357/effects-of-inbreeding-and-other-systematic-effects-on-fertility-of-black-forest-draught-horses-in-germany
#10
Maarit Müller-Unterberg, Sandra Wallmann, Ottmar Distl
BACKGROUND: The Black Forest Draught horse (BFDH) is an endangered German coldblood breed with its origin in the area of the Black Forest in South Germany. In this retrospective study, the influence of the inbreeding coefficient on foaling rates was investigated using records from ten breeding seasons. Due to the small population size of BFDH, the level of inbreeding is increasing and may have an effect on foaling rates.The data of the present study included all coverings reported for 1024 BFDH mares in the years 2001-2009...
October 18, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29035837/testicular-cytology-by-fine-needle-aspiration-in-domestic-cats
#11
D P Leme, E Visacre, V B Castro, M D Lopes
In cases where semen collection in tom-cats is not possible, FNA of testes is the alternative to evaluate sperm production. Although this technique for the diagnosis of fertility problems has been well discussed in other mammals (men, dogs, stallions), data for domestic cats are limited. Therefore, the aim of this study was to verify the reliability of FNA using needles of small diameters (22G and 29G) in testes of domestic cats of different ages to assess the spermatogenesis status and to present description of germ cells and Sertoli cells for cytological examinations...
October 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/28987795/morphological-evaluation-of-day-8-embryos-developed-during-induced-aluteal-cycles-in-the-mare
#12
C A Leisinger, V Medina, M L Markle, D L Paccamonti, C R F Pinto
A novel in vivo model utilizing serial administrations of PGF2α to induce aluteal cycles in the mare was used to evaluate the effects of progesterone-deprivation on the morphology of in vivo preimplantation embryos. We hypothesized that equine embryos produced during induced aluteal cycles (AL) would be developmentally affected, characterized by earlier embryo stage at collection, smaller embryo diameter, and lower quality grade, compared with those collected on the same day post-ovulation from control cycles during diestrus (high progesterone; > 4 ng/mL)...
January 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/28986135/impact-of-supplementation-of-semen-extender-with-antioxidants-on-the-quality-of-chilled-or-cryopreserved-arabian-stallion-spermatozoa
#13
sAbdelRaouf M Ghallab, Abdallah M Shahat, Aya M Fadl, Mohamed M Ayoub, Adel R Moawad
The aim of the present study was to evaluate the effects of supplementation of semen extender with various non-enzymatic antioxidants on the quality of cooled or cryopreserved Arabian stallion spermatozoa. Semen collected from four pure Arabian stallions was centrifuged at 600g for 15 min. Spermatozoa were then diluted in INRA-82 extender supplemented with bovine serum albumin (BSA; 0, 10, 15 and 20 mg/mL) or trehalose (0, 75, 100 and 150 mM) or zinc sulphate (0, 100, 150 and 200 μM). The diluted semen was then either cooled at 5 °C or cryopreserved in 0...
October 4, 2017: Cryobiology
https://www.readbyqxmd.com/read/28986134/consequences-of-adding-gum-arabic-as-a-cryoprotectant-on-motility-and-viability-of-frozen-stallion-semen
#14
Mohamed Ali, Musa M Musa, Sulaiman Alfadul, K Al-Sobayel
A trial was conducted to check effect of adding gum Arabic (GA) instead of egg yolk (EY) as a cryoprotectant for stallion sperm. Two experiments were designed; experiment I tested adding 3 levels of nonheated GA (i.e., 3, 6 and 9 g/100 mL diluents) in HF-20 extender. However, in experiment II the same levels were tested except that GA was heated at 80 °C for 60 min. HF-20 containing 10% of EY was used as control. In experiment I, sperm frozen in HF-20 containing nonheated GA exhibited lower percentages of motile sperm, progressively motile sperm and sperm with intact plasma membranes, vitality rate, and acrosome integrity after cooling or after deep freezing...
October 3, 2017: Cryobiology
https://www.readbyqxmd.com/read/28973824/stallion-spermatozoa-surviving-freezing-and-thawing-experience-membrane-depolarization-and-increased-intracellular-na
#15
C Ortega Ferrusola, L Anel-López, J M Ortiz-Rodriguez, P Martin Muñoz, M Alvarez, P de Paz, J Masot, E Redondo, C Balao da Silva, J M Morrell, H Rodriguez Martinez, J A Tapia, M C Gil, L Anel, F J Peña
In order to gain insight of the modifications that freezing and thawing cause to the surviving population of spermatozoa, changes in the potential of the plasma membrane (Em) and intracellular Na(+) content of stallion spermatozoa were investigated using flow cytometry. Moreover, caspase 3 activity was also investigated and the functionality of the Na(+) -K(+) ATPase pump was investigated before and after freezing and thawing. Cryopreservation caused a significant (p < 0.001) increase in the subpopulation of spermatozoa with depolarized sperm membranes, concomitantly with an increase (p < 0...
October 3, 2017: Andrology
https://www.readbyqxmd.com/read/28971522/half-a-century-of-equine-reproduction-research-and-application-a-veterinary-tour-de-force
#16
REVIEW
Twink W R Allen, S Wilsher
Over the past 50 years, pregnancy rates in Thoroughbred mares have risen from 70 to >90% and foaling rates from 55 to >80%. Some of the significant pharmacological treatments and diagnostic methods which have driven this marked improvement in breeding efficiency are reviewed. They include the application of artificial lighting to hasten the onset of ovulatory oestrous cyclicity in early Spring, rapid steroid hormone assays to aid in determining the stage and normality of the mare's cycle, prostaglandin analogues, synthetic progestagens and Gonadotrophin-releasing Hormone (GnRH) analogues to better control and manipulate her cycle to good effect, transrectal ultrasound scanning to monitor follicle growth, endometrial architecture and ovulation and to allow accurate, early pregnancy diagnosis thereby enabling successful ablation of one of twin conceptuses...
October 3, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28969775/factors-affecting-prevalence-and-abundance-of-a-perfoliata-infections-in-horses-from-south-eastern-poland
#17
Krzysztof Tomczuk, Maciej Grzybek, Klaudiusz Szczepaniak, Maria Studzińska, Marta Demkowska-Kutrzepa, Monika Roczeń-Karczmarz, Zahrai Abdulhammza Abbass, Krzysztof Kostro, Andrzej Junkuszew
Equine Anoplocephalosis constitute a significant problem in horses worldwide. The aim of this study was to analyse intrinsic (host age and sex) and extrinsic (management type, pasture type and moisture) factors that influence the prevalence and FEC of A. perfoliata infections. Faecal samples were collected from 994 horses managed in studs or individually between 2012 and 2014. The Sedimentation-flotation method was applied for coproscopic analysis, and faecal egg counts were calculated. The overall prevalence was 25...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28962880/do-olfactory-behaviour-and-marking-responses-of-konik-polski-stallions-to-faeces-from-conspecifics-of-either-sex-differ
#18
Tadeusz Jezierski, Zbigniew Jaworski, Magdalena Sobczyńska, John Ensminger, Aleksandra Górecka-Bruzda
The aim of the study was to assess whether adult stallions differentiate their olfactory and marking behaviour towards the excreta of their potential male rivals and mares. Four Konik polski stallions were individually exposed simultaneously to their own and each others' faeces as well as faeces of mares in oestrus vs. dioestrus. Five series of 30min observation sessions were conducted in a round pen each on two consecutive days from March to July, totaling in 5h observation per stallion. Stallions sniffed and displayed flehmen reaction towards mares' faeces significantly longer (P<0...
September 27, 2017: Behavioural Processes
https://www.readbyqxmd.com/read/28930953/proposed-clinical-pathway-for-nonoperative-management-of-high-grade-pediatric-pancreatic-injuries-based-on-a-multicenter-analysis-a-pediatric-trauma-society-collaborative
#19
MULTICENTER STUDY
Bindi J Naik-Mathuria, Eric H Rosenfeld, Ankush Gosain, Randall Burd, Richard A Falcone, Rajan Thakkar, Barbara Gaines, David Mooney, Mauricio Escobar, Mubeen Jafri, Anthony Stallion, Denise B Klinkner, Robert Russell, Brendan Campbell, Rita V Burke, Jeffrey Upperman, David Juang, Shawn St Peter, Stephon J Fenton, Marianne Beaudin, Hale Wills, Adam Vogel, Stephanie Polites, Adam Pattyn, Christine Leeper, Laura V Veras, Ilan Maizlin, Shefali Thaker, Alexis Smith, Megan Waddell, Joseph Drews, James Gilmore, Lindsey Armstrong, Alexis Sandler, Suzanne Moody, Brandon Behrens, Laurence Carmant
BACKGROUND: Guidelines for nonoperative management (NOM) of high-grade pancreatic injuries in children have not been established, and wide practice variability exists. The purpose of this study was to evaluate common clinical strategies across multiple pediatric trauma centers to develop a consensus-based standard clinical pathway. METHODS: A multicenter, retrospective review was conducted of children with high-grade (American Association of Surgeons for Trauma grade III-V) pancreatic injuries treated with NOM between 2010 and 2015...
October 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28921680/measurement-of-urinary-concentrations-of-the-mycotoxins-zearalenone-and-sterigmatocystin-as-biomarkers-of-exposure-in%C3%A2-mares
#20
M Takagi, S Uno, E Kokushi, F Sato, Mmp Wijayagunawardane, J Fink-Gremmels
Mycotoxins may affect animal health, including reproduction. Little is known about the clinical relevance of exposure of horses to contaminated feed. This study aimed at (i) monitoring the levels of the mycotoxins zearalenone (ZEN), with its metabolites α- and β-zearalenol (α- and β-ZOL), and sterigmatocystin (STC) in urine samples from thoroughbred mares in Japan and (ii) relating these findings to the potential effects on reproductive efficacy of breeding mares. Sixty-three urine samples of breeding mares from 59 breeding farms were used...
September 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
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