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https://www.readbyqxmd.com/read/28921680/measurement-of-urinary-concentrations-of-the-mycotoxins-zearalenone-and-sterigmatocystin-as-biomarkers-of-exposure-in%C3%A2-mares
#1
M Takagi, S Uno, E Kokushi, F Sato, Mmp Wijayagunawardane, J Fink-Gremmels
Mycotoxins may affect animal health, including reproduction. Little is known about the clinical relevance of exposure of horses to contaminated feed. This study aimed at (i) monitoring the levels of the mycotoxins zearalenone (ZEN), with its metabolites α- and β-zearalenol (α- and β-ZOL), and sterigmatocystin (STC) in urine samples from thoroughbred mares in Japan and (ii) relating these findings to the potential effects on reproductive efficacy of breeding mares. Sixty-three urine samples of breeding mares from 59 breeding farms were used...
September 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28855043/association-of-the-cysteine-rich-secretory-protein-3-crisp-3-and-some-of-its-polymorphisms-with-the-quality-of-cryopreserved-stallion-semen
#2
Alexandra Usuga, Benjamín A Rojano, Giovanni Restrepo
Contribution of seminal plasma proteins to semen freezability has been reported in several species, suggesting these proteins as genetic markers. The aim of this study was to evaluate the relationship between cysteine-rich secretory protein-3 (CRISP-3) and some of its single-nucleotide polymorphisms (SNPs) with post-thawing semen quality in stallions. DNA was obtained from 100 stallions, regions of interest were amplified by polymerase chain reaction and sequenced. Evaluated SNPs within the equine CRISP-3 gene were CRISP3c...
August 31, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28846911/testicular-volumetry-and-prediction-of-daily-sperm-output-in-stallions-by-orchidometry-and-two-and-three-dimensional-sonography
#3
S Pricking, H Bollwein, K Spilker, G Martinsson, A Schweizer, S Thomas, H Oldenhof, H Sieme
Accurate determination of the testes volume and prediction of the daily sperm output (DSO) is valuable information for reproductive management of a stallion. The aim of this study was to compare different methods for measuring the testes volume, including caliper, 2D and 3D ultrasound. Special emphasis was on feasibility of 3D volume analysis. First, 22 castrated testes were measured and derived volumes were compared with volumes determined via volume displacement in a graded cylinder with saline solution. Then, during the breeding season, testes sizes of 52 stallions were measured in vivo and analyzed...
August 19, 2017: Theriogenology
https://www.readbyqxmd.com/read/28833663/redox-cycling-induces-spermptosis-and-necrosis-in-stallion-spermatozoa-while-the-hydroxyl-radical-oh%C3%A2-only-induces-spermptosis
#4
P Martín Muñoz, L Anel-López, J M Ortiz-Rodríguez, M Álvarez, P de Paz, C Balao da Silva, H Rodríguez Martinez, M C Gil, L Anel, F J Peña, C Ortega Ferrusola
Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2-methyl-1,4-naphthoquinone (menadione), or hydroxyl radical formation after FeSO4 exposure...
August 17, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28827080/stallion-sperm-selection-prior-to-freezing-using-a-modified-colloid-swim-up-procedure-without-centrifugation
#5
M Hidalgo, I Ortiz, J Dorado, J M Morrell, J Gosálvez, C Consuegra, M Diaz-Jimenez, B Pereira, F Crespo
The aims of this study were to: 1) develop a new method for stallion sperm selection using a modified swim-up procedure through a colloid and 2) evaluate its impact in good quality ejaculates from bad freezers in comparison to methods involving centrifugation such as single layer centrifugation and sperm washing. Ejaculates were processed before freezing using three different procedures: sperm washing (SW), colloid single layer centrifugation (SLC) and a modified colloid swim-up (SU). After semen processing, sperm recovery rates were measured and sperm were frozen...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28814389/equine-arteritis-virus-elicits-a-mucosal-antibody-response-in-the-reproductive-tract-of-persistently-infected-stallions
#6
Mariano Carossino, Bettina Wagner, Alan T Loynachan, R Frank Cook, Igor F Canisso, Lakshman Chelvarajan, Casey L Edwards, Bora Nam, John F Timoney, Peter J Timoney, Udeni B R Balasuriya
Equine arteritis virus (EAV) has the ability to establish persistent infection in the reproductive tract of the stallion (carrier) and is continuously shed in its semen. We have recently demonstrated that EAV persists within stromal cells and a subset of lymphocytes in the stallion accessory sex glands in the presence of a significant local inflammatory response. In the present study, we demonstrated that EAV elicits a mucosal antibody response in the reproductive tract during persistent infection with homing of plasma cells into accessory sex glands...
August 16, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28805449/stallion-sperm-cryopreservation-using-various-permeating-agents-interplay-between-concentration-and-cooling-rate
#7
Harriëtte Oldenhof, Judith Bigalk, Christiane Hettel, Lawrence de Oliveira Barros, Bulat Sydykov, Árpád Cs Bajcsy, Harald Sieme, Willem F Wolkers
In this study, modeling and experimental approaches were used to investigate the interplay between cooling rate and protectant concentration for cryopreservation of stallion sperm. Glycerol (GLY), ethylene glycol (EG), dimethylformamide (DMF), propylene glycol (PG), and dimethyl sulfoxide (DMSO) were tested as cryoprotective agents (CPAs), using concentrations up to 1500 mM and cooling rates ranging from 5°C to 55°C min(-1). Modeling of the extent of sperm dehydration during freezing was done using previously determined values of the sperm membrane permeability to water to predict optimal cooling rates for cryopreservation...
August 14, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28804963/effect-of-housing-system-on-reproductive-behaviour-and-on-some-endocrinological-and-seminal-parameters-of-donkey-stallions
#8
A Rota, M Sgorbini, D Panzani, F Bonelli, P Baragli, N Ille, D Gatta, C Sighieri, L Casini, M M Maggiorelli, C Aurich, F Camillo
Reproductive management of male donkeys employed for artificial breeding has been poorly studied. The aim of this study was to evaluate the effect of housing system, with the animals grouped together in a paddock or kept in individual boxes, on sexual behaviour, cortisol and testosterone concentration and seminal characteristics of adult male donkeys. The study included four Amiata donkey jacks (stallions) from which ejaculates, saliva and blood were collected during two distinct 3 weeks periods, one in the group and one in the box housing system...
August 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28772242/the-relationship-between-the-welfare-quality-and-stress-index-in-working-and-breeding-horses
#9
Silvana Popescu, Eva A Diugan
The aim of this observational study was to investigate if a relationship exists between the calculated overall individual welfare score (as an indicator of the welfare quality) and the neutrophil:lymphocyte (N:L) ratio (as a stress indicator) in working (draught) and reproduction horses (breeding stallions and broodmares), in two different seasons. The welfare of the horses was assessed by a protocol which included health and behavioural parameters. An individual welfare score was computed, the total and differential WBC count was established and the N:L ratio was calculated for each of the 1482 assessed horses...
July 26, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28762781/application-of-the-hands-on-donkey-tool-for-assessing-the-welfare-of-working-equids-at-tuliman-mexico
#10
F Galindo, A de Aluja, R Cagigas, L A Huerta, T A Tadich
Equids are still used for diverse chores in Mexico and are essential for the livelihoods of numerous families. Appropriate health and behavior are prerequisites for performing work without affecting welfare. This study aimed to assess the welfare of working equids in Tuliman, applying the hands-on donkey tool. This tool evaluates five dimensions (behavior, body condition score [BCS], wounds, lameness, and other health issues) and was applied to 438 working equids (horses, mules, and donkeys). The Kruskall-Wallis test was applied to investigate differences between species and sex...
August 1, 2017: Journal of Applied Animal Welfare Science: JAAWS
https://www.readbyqxmd.com/read/28753433/effect-of-kisspeptin-10-lh-and-hcg-on-serum-testosterone-concentrations-in-stallions-donkeys-and-mules
#11
Rana Waseem Akhtar, Syed Aftab Hussain Shah, Irfan Zia Qureshi
This study was conducted to determine the response of serum testosterone (T) in male equines (stallions, donkeys and mules) after administering intravenous doses of kisspeptin-10 (KP-10), human chorionic gonadotropin (hCG) and luteinizing hormone (LH) and saline as a control. The animals were divided into four groups of three each: Group I, 3 ml of 0.95% saline; Group II, 50 μg KP-10; Group III, 2500 IU hCG and group IV, 400 μg LH. The administration of KP-10 and hCG to stallions resulted in a significant increase in serum T concentration at 240 min; whereas it was significantly higher at 30, 60, 120, and 240 min with LH treatment as compared to pre-dose concentrations...
July 21, 2017: Theriogenology
https://www.readbyqxmd.com/read/28730713/investigation-of-genetic-diversity-and-inbreeding-in-a-japanese-native-horse-breed-for-suggestions-on-its-conservation
#12
Akio Onogi, Kouichi Shirai, Tomoko Amano
Because native breeds can serve as genetic resources for adapting to environment changes, their conservation is important for future agroecosystems. Using pedigree analysis, we investigated genetic diversity and inbreeding in Japanese Hokkaido native horses, which have adapted to a cold climate and roughage diet. Genetic diversity was measured as the number of founders and the effective number of founders, ancestors and genomes. All metrics imply a decrease in genetic diversity. A comparison of these metrics suggested that pedigree bottlenecks contributed more than did random gene losses to the reduction of genetic diversity...
July 21, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28708505/seasonal-changes-of-dna-fragmentation-and-quality-of-raw-and-cold-stored-stallion-spermatozoa
#13
L Wach-Gygax, D Burger, E Malama, H Bollwein, A Fleisch, E Jeannerat, S Thomas, G Schuler, F Janett
In this study annual fluctuations of DNA fragmentation and quality of cold-stored equine sperm were evaluated. Ejaculates were collected weekly during one year from 15 stallions. Ejaculate volume, sperm concentration and total sperm count were determined and semen was then extended and cold-stored for 48 h. Sperm motility was evaluated by CASA before and after 24 as well as 48 h of cold storage. In addition, the percentages of sperm with intact plasma membrane and acrosome (PMAI %) and with low intracellular Ca(2+) level were determined in cold-stored semen (24 h, 48 h)...
May 30, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708494/relationship-between-echotextural-and-histomorphometric-characteristics-of-stallion-testes
#14
Malgorzata Pozor, Heather Morrissey, Valeria Albanese, Natalie Khouzam, Alexis Deriberprey, Margo L Macpherson, Audrey A Kelleman
The goal of this study was to investigate correlations between objective measures of testicular echotexture and histomorphometric attributes related to the histological composition of stallion testes. Fifty-four scrotal testes were obtained from three groups of stallions during routine castrations: colts <1 yr old (n = 18), young stallions 1-5 yrs old (n = 27), mature stallions > 5 yrs old (n = 9). In addition, two scrotal testes with degeneration, 16 retained inguinal and 10 retained abdominal testes were surgically obtained...
June 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28697876/pharmacokinetics-of-the-anti-androgenic-drug-flutamide-in-healthy-stallions
#15
Francisco Javier Mendoza, Juan Manuel Serrano-Rodriguez, Antonio Buzon-Cuevas, Alejandro Perez-Ecija
Alternatives to surgical castration are necessary for controlling the sexual behaviour of stallions with breeding potential in training and competition. Flutamide is a potent selective non-steroidal androgen receptor competitive antagonist that has been used in human beings as an anti-androgenic drug. In this study, the pharmacokinetics and bioavailability of flutamide and its main active metabolite, 2-hydroflutamide, were determined in seven healthy mature stallions. Single doses of flutamide (1mg/kg intravenously, 1mg/kg orally in fasted horses, 5mg/kg orally in fasted horses and 5mg/kg orally in fed horses) were administered randomly at intervals of 2 weeks...
June 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28669755/y-chromosome-uncovers-the-recent-oriental-origin-of-modern-stallions
#16
Barbara Wallner, Nicola Palmieri, Claus Vogl, Doris Rigler, Elif Bozlak, Thomas Druml, Vidhya Jagannathan, Tosso Leeb, Ruedi Fries, Jens Tetens, Georg Thaller, Julia Metzger, Ottmar Distl, Gabriella Lindgren, Carl-Johan Rubin, Leif Andersson, Robert Schaefer, Molly McCue, Markus Neuditschko, Stefan Rieder, Christian Schlötterer, Gottfried Brem
The Y chromosome directly reflects male genealogies, but the extremely low Y chromosome sequence diversity in horses has prevented the reconstruction of stallion genealogies [1, 2]. Here, we resolve the first Y chromosome genealogy of modern horses by screening 1.46 Mb of the male-specific region of the Y chromosome (MSY) in 52 horses from 21 breeds. Based on highly accurate pedigree data, we estimated the de novo mutation rate of the horse MSY and showed that various modern horse Y chromosome lineages split much later than the domestication of the species...
July 10, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28619163/frequent-occurrence-of-nonprimate-hepacivirus-infections-in-thoroughbred-breeding-horses-a-cross-sectional-study-for-the-occurrence-of-infections-and-potential-risk-factors
#17
Claudia Reichert, Amely Campe, Stephanie Walter, Stephanie Pfaender, Kathrin Welsch, Inga Ruddat, Harald Sieme, Karsten Feige, Eike Steinmann, Jessika M V Cavalleri
Recently, several new hepaciviruses have been identified of which the nonprimate hepacivirus (NPHV) - the closest relative to hepatitis C virus (HCV) discovered to date - is highly prevalent in horses. However, potential risk factors for the transmission of NPHV among horses remain still unknown. Therefore, the objective of this study was to investigate the occurrence of NPHV infections in Thoroughbreds in northern and western Germany and to identify potential risk factors associated with NPHV infections. Using a cross-sectional study design, a total of 733 serum samples from Thoroughbred broodmares and stallions from northern and western Germany were analyzed for the presence of anti-NPHV nonstructural protein 3 (NS3) antibodies and NPHV RNA using the luciferase immunoprecipitation system (LIPS) and a quantitative real-time PCR, respectively...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28601147/deep-anestrous-mares-under-natural-photoperiod-treated-with-recombinant-equine-fsh-refsh-and-lh-relh-have-fertile-ovulations-and-become-pregnant
#18
Geraldine A Meyers-Brown, Meaghan C Loud, John C Hyland, Janet F Roser
The most common equine breeding practice to decrease the time to the first ovulation of the year is to use artificial lights starting December 1 in the Northern Hemisphere. It can take 60-90 d for this lighting regimen to induce a fertile ovulation. The success rate for pharmaceutical compounds to carry out the same process has been variable. One compound that did induce an early ovulation was recombinant equine follicle stimulating hormone (reFSH), but neither pregnancy nor cyclicity was established in that study...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28590353/proposed-clinical-pathway-for-non-operative-management-of-high-grade-pediatric-pancreatic-injuries-based-on-a-multicenter-analysis-a-pediatric-trauma-society-collaborative
#19
Bindi J Naik-Mathuria, Eric H Rosenfeld, Adam Vogel, Ankush Gosain, Randall Burd, Richard A Falcone, Rajan Thakkar, Barbara Gaines, David Mooney, Mauricio Escobar, Mubeen Jafri, Anthony Stallion, Denise B Klinkner, Robert Russell, Brendan Campbell, Rita V Burke, Jeffrey Upperman, David Juang, Shawn St Peter, Stephon J Fenton, Marianne Beaudin, Hale Wills, Stephanie Polites, Adam Pattyn, Christine Leeper, Laura V Veras, Ilan Maizlin, Shefali Thaker, Alexis Smith, Megan Waddell, Joseph Drews, James Gilmore, Lindsey Armstrong, Alexis Alex Ler, Suzanne Moody, Brandon Behrens, Laurence Carmant
BACKGROUND: Guidelines for non-operative management (NOM) of high-grade pancreatic injuries in children have not been established, and wide practice variability exists. The purpose of this study was to evaluate common clinical strategies across multiple pediatric trauma centers in order to develop a consensus-based standard clinical pathway. METHODS: A multicenter, retrospective review was conducted of children with high-grade (AAST grade III-V) pancreatic injuries treated with NOM between 2010-15...
June 6, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28583604/prediction-of-the-fertility-of-stallion-frozen-thawed-semen-using-a-combination-of-computer-assisted-motility-analysis-microscopical-observation-and-flow-cytometry
#20
I Barrier Battut, A Kempfer, N Lemasson, L Chevrier, S Camugli
Spermatozoa from some stallions do not maintain an acceptable fertility after freezing and thawing. The selection of frozen ejaculates that would be suitable for insemination is mainly based on post-thaw motility, but the prediction of fertility remains limited. A recent study in our laboratory has enabled the determination of a new protocol for the evaluation of fresh stallion semen, combining microscopical observation, computer-assisted motility analysis and flow cytometry, and providing a high level of fertility prediction...
July 15, 2017: Theriogenology
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