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https://www.readbyqxmd.com/read/29784463/anti-m%C3%A3-llerian-hormone-profiling-in-prepubertal-horses-and-its-relationship-with-gonadal-function
#1
Dragos Scarlet, Manuela Wulf, Juliane Kuhl, Martin Köhne, Natascha Ille, Alan J Conley, Christine Aurich
Anti-Müllerian hormone (AMH) has gained increasing interest as a biomarker for assessment of gonadal activity. The ability to predict the ovarian follicular reserve of prepubertal female horses (fillies) or to identify stallions with testicular pathologies already during their prepubertal life has not been analyzed so far. Both would help to select fertile horses and reduce costs associated with keeping animals. The objectives of the present study were to (1) assess AMH, LH, FSH, progesterone (females) and testosterone (males) dynamics in prepubertal horses from birth onwards and (2) determine whether AMH concentrations detected in plasma of prepubertal female and male horses are correlated with postpubertal gonadal development...
May 15, 2018: Theriogenology
https://www.readbyqxmd.com/read/29779880/in-vitro-comparison-of-acyclovir-ganciclovir-and-cidofovir-against-equid-alphaherpesvirus-3-and-evaluation-of-their-efficacy-against-six-field-isolates
#2
María A Vissani, Osvaldo Zabal, María S Tordoya, Viviana Parreño, Etienne Thiry, María Barrandeguy
Equid alphaherpesvirus 3 (EHV3) is the etiological agent of equine coital exanthema (ECE), which is a venereal, highly contagious disease, characterized by the formation of papules, vesicles, pustules and ulcers on the external genitalia of mares and stallions. EHV3 remains in a latent state after a successful infection and there are latently infected animals in which the virus is reactivated and generally re-excreted subclinically. There are no available vaccines for this condition and prevention is based on the clinical examination of mares prior to mating, which allows to segregate those showing clinical signs...
May 17, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29766270/parasitological-and-molecular-diagnostic-of-a-clinical-babesia-caballi-outbreak-in-southern-romania
#3
Mariana Ionita, Isabela Madalina Nicorescu, Kurt Pfister, Ioan Liviu Mitrea
Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Babesia caballi and/or Theileria equi, which is endemic in many tropical and temperate areas of the world. However, clinical outbreaks of EP in Romania during the last decades have not been reported Therefore, the aim of this paper is (i) to describe a clinical B. caballi outbreak in horses on several farms in Southern Romania using a diagnostic and therapeutic approach and (ii) the molecular diagnostic of EP in an endemic area of Romania...
May 15, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29763439/marginal-ancestral-contributions-to-atrial-fibrillation-in-the-standardbred-racehorse-comparison-of-cases-and-controls
#4
Megan Kraus, Peter Physick-Sheard, Luiz F Brito, Mehdi Sargolzaei, Flávio S Schenkel
Admissions of Standardbred racehorses (Std) to the Ontario Veterinary College Teaching Hospital (OVCTH) for treatment of atrial fibrillation (AF) began to increase in the early 1990s. The arrhythmia has been shown to have a modest heritability (h2 ≃ 0.15), with some stallions appearing as sires or sires of mares used in breeding (broodmares) of affected horses more frequently than others. The objective of this study was to determine the marginal genetic contributions of ancestors to cohorts of Std affected with AF and their contemporary control groups, and whether these ancestors contribute significantly more to the affected cohorts than to controls...
2018: PloS One
https://www.readbyqxmd.com/read/29761547/a-novel-combination-of-silane-coated-silica-colloid-with-hybrid-rna-extraction-protocol-and-rna-enrichment-for-downstream-applications-of-spermatozoal-rna
#5
K Vijayalakshmy, P Kumar, M Virmani, S Pawaria, N S Lalaji, P Sharma, R Rajendran, P S Yadav, D Kumar
Spermatozoa are specialised cells with low RNA content as compared to somatic cells. The suitable sperm RNA extraction and enrichment protocols for downstream applications are available for human, cattle, stallion and mouse but not for buffalo spermatozoa. Therefore, the present work was conducted to find out suitable colloidal solution for sperm purification and appropriate protocol for sperm RNA extraction and enrichment/amplification of RNA. For purification, we used PVP-coated silica colloidal solution (PVP-Si), silane-coated silica colloidal solution (Silane-Si) and iodixanol...
May 14, 2018: Andrologia
https://www.readbyqxmd.com/read/29747159/seminal-plasma-influences-the-fertilizing-potential-of-cryopreserved-stallion-sperm
#6
Essraa M Al-Essawe, Margareta Wallgren, Manuela Wulf, Christine Aurich, Beatriz Macías-García, Ylva Sjunnesson, Jane M Morrell
Seminal plasma (SP) contains proteins that may influence cryosurvival and prevent capacitation-like changes due to freezing and thawing. The objective of this study was to investigate the effect of adding pooled SP from "good" (GF) or "bad" (BF) freezer stallions on sperm cells' fertilizing ability. "Good freezers" refers to stallions that usually produce ejaculates which can withstand cryopreservation, whilst "bad freezer" stallions produce ejaculates which cannot tolerate the freezing process...
April 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29734417/variable-inter-assay-estimation-of-sperm-dna-fragmentation-in-stallions-classified-as-good-and-bad-semen-freezers
#7
H N Ferreira, J C Ferreira-Silva, J M Rocha, M C Farras, M Calixto, M T Moura, M A Alvarenga, A L Oliveira
BACKGROUND: Semen cryopreservation causes DNA damage, thus requiring continuous monitoring. OBJECTIVE: To compare two assays for sperm DNA fragmentation (SDF) from stallions with contrasting semen freezability. MATERIALS AND METHODS: Thirteen stallions were classified as good semen freezers (GSF) or bad semen freezers (BSF). Ejaculates were cryopreserved with three diluents. Semen was subject to SDF evaluation using the sperm chromatin structure assay (SCSA) and Halomax after thawing (0 h) and after a 4 h thermoresistance test...
January 2018: Cryo Letters
https://www.readbyqxmd.com/read/29716644/equine-strongyle-communities-are-constrained-by-horse-sex-and-species-dipersal-fecundity-trade-off
#8
Guillaume Sallé, Sławomir Kornaś, Marta Basiaga
BACKGROUND: Equine strongyles are a major health issue. Large strongyles can cause death of horses while cyathostomins (small strongyles) have shown increased resistance to anthelmintics worldwide. Description of strongyle communities have accumulated but little is known about the diversity of these communities and underpinning environmental factors. METHODS: Strongyles were recovered after ivermectin treatment from 48 horses located in six premises in Poland. Correlation between previously published species fecundity and the observed relative abundance and prevalence were estimated...
May 2, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29675468/decline-of-genetic-diversity-in-ancient-domestic-stallions-in-europe
#9
Saskia Wutke, Edson Sandoval-Castellanos, Norbert Benecke, Hans-Jürgen Döhle, Susanne Friederich, Javier Gonzalez, Michael Hofreiter, Lembi Lõugas, Ola Magnell, Anna-Sapfo Malaspinas, Arturo Morales-Muñiz, Ludovic Orlando, Monika Reissmann, Alexandra Trinks, Arne Ludwig
Present-day domestic horses are immensely diverse in their maternally inherited mitochondrial DNA, yet they show very little variation on their paternally inherited Y chromosome. Although it has recently been shown that Y chromosomal diversity in domestic horses was higher at least until the Iron Age, when and why this diversity disappeared remain controversial questions. We genotyped 16 recently discovered Y chromosomal single-nucleotide polymorphisms in 96 ancient Eurasian stallions spanning the early domestication stages (Copper and Bronze Age) to the Middle Ages...
April 2018: Science Advances
https://www.readbyqxmd.com/read/29660154/local-mepivacaine-before-castration-of-horses-under-medetomidine-isoflurane-balanced-anaesthesia-is-effective-to-reduce-perioperative-nociception-and-cytokine-release
#10
M Abass, S Picek, J F Glaus Carzòn, C Kühnle, A Zaghlou, R Bettschart-Wolfensberger
BACKGROUND: In horses castration with primary intention healing is usually performed under balanced inhalation anaesthesia. To optimise analgesia, the use of local anaesthesia was tested. OBJECTIVES: To investigate the effect of local mepivacaine before castration with first intention healing under balanced medetomidine-isoflurane anaesthesia and flunixin meglumine, morphine analgesia on perioperative cytokine levels and pain in horses. STUDY DESIGN: Prospective blinded clinical study...
April 16, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29658099/identification-of-serotoninergic-system-components-in-stallion-sperm
#11
F Jiménez-Trejo, I Coronado-Mares, M Boeta, I González-Santoyo, R Vigueras-Villaseñor, C Arriaga-Canon, L Herrera-Montalvo, M Tapia-Rodríguez
Assisted reproductive techniques have been used on several domestic animals to preserve desirable traits in strains of high genetic and commercial value; however in equines its efficiency rate is relatively low. To increase the conception ratio in stallions, some research groups have used pharmacological treatments which promote sperm hyperactivation in order to increase male's fertility rates. In this way, our previous work suggests that serotonin (5-HT) could be a good pharmacological candidate that facilitates conception rate in domestic horses...
April 16, 2018: Histology and Histopathology
https://www.readbyqxmd.com/read/29653389/how-does-the-microbial-load-affect-the-quality-of-equine-cool-stored-semen
#12
E Varela, J Rey, E Plaza, P Muñoz de Propios, J M Ortiz-Rodríguez, M Álvarez, L Anel-López, L Anel, P De Paz, M C Gil, J M Morrell, C Ortega-Ferrusola
Contaminating bacteria present in stallion ejaculates may compromise sperm quality during storage. Different procedures have been used to reduce the load of microorganisms in semen and avoid bacterial growth during storage. The aims of this study were: 1) to evaluate different techniques to eliminate bacteria in semen 2) to study the relationship between total microflora load (TML) and ROS production; and 3) to determine if TML affects the functionality of cool-stored sperm. Ejaculates from 11 stallions were split and processed in 3 ways: A...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29627335/on-site-detection-of-equid-alphaherpesvirus-3-in-perineal-and-genital-swabs-of-mares-and-stallions
#13
M A Vissani, M S Tordoya, Y-L Tsai, P-Y A Lee, Y-H Shen, F-C Lee, H-T T Wang, V Parreño, M Barrandeguy
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species...
April 5, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29622352/simultaneous-evaluation-of-superoxide-content-and-mitochondrial-membrane-potential-in-stallion-semen-samples-provides-additional-information-about-sperm-quality
#14
A Johannisson, M I Figueiredo, Z Al-Kass, J M Morrell
An improved fertility prediction for stallions is of importance for equine breeding. Here, we investigate the potential of a combined staining of stallion spermatozoa for superoxide and mitochondrial membrane potential (MMP) for this purpose. Semen samples were analysed immediately after arrival at the laboratory, as well as after 24 h. Superoxide was measured by MitoSOXRed, while MMP was measured with JC-1. Menadione was used to stimulate superoxide production. In addition, other parameters of sperm quality, namely motility, membrane integrity, chromatin integrity, sperm kinematics and Hoechst 33258 exclusion were measured and correlated to superoxide production and MMP...
March 29, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29597125/the-anti-inflammatory-effect-of-exogenous-lactoferrin-on-breeding-induced-endometritis-in-susceptible-mares-when-administered-post-breeding-in-susceptible-mares
#15
C E Fedorka, K E Scoggin, Y L Boakari, N E Hoppe, E L Squires, B A Ball, M H T Troedsson
The deposition of semen into the uterus of the horse induces a transient innate immune response that lasts 24-36 h in the normal mare. There exists a subset of mares that are unable to resolve this inflammation in a timely manner, and are classified as susceptible to the disease of persistent breeding-induced endometritis (PBIE). Lactoferrin is a protein of interest as a potential therapeutic for this persistent inflammation due to its anti-inflammatory and bactericidal properties. The addition of human recombinant lactoferrin (hrLF) to the insemination dose was previously shown to suppress mRNA expression of the pro-inflammatory cytokine tumor necrosis factor (TNF)-α at 6 h after insemination, but no studies have shown the effect of lactoferrin when infused post-breeding...
March 22, 2018: Theriogenology
https://www.readbyqxmd.com/read/29572335/effect-of-horse-gender-on-british-eventing-competition-performance-an-observational-study-between-1998-and-2016
#16
Katherine Hanousek, Mazdak Salavati, Ali Fouladi-Nashta
To test the hypothesis that gender affects horse scores in eventing competition, data on the scores and points awarded to 681 horses was collected from the British Eventing website. Equal numbers of mares, geldings and stallions were used, all foaled during or after 1994 and aged 4-10 years. The study included five levels of competition (BE90, BE100, Novice, Intermediate and Advanced) and investigated differences in mean phased scores, total scores and rank in competition. Additionally, the mean and median 'BE points per competition' of each gender were compared...
March 23, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29534827/effects-of-immunization-against-bone-morphogenetic-protein-15-and-growth-differentiation-factor-9-on-ovarian-function-in-mares
#17
Kelli A Davis, Kristin M Klohonatz, Darcy S O Mora, Hannah M Twenter, Peter E Graham, Pablo Pinedo, Douglas C Eckery, Jason E Bruemmer
Currently there is no contraceptive vaccine that can cause permanent sterility in mares. This study investigates the effect of vaccination against oocyte-specific growth factors, Bone Morphogenetic Protein 15 (BMP-15) and Growth Differentiation Factor 9 (GDF-9), on ovarian function of mares. It was hypothesized that immunization against these growth factors would prevent ovulation and/or accelerate depletion of the oocyte reserve. For this study, 30 mares were randomly assigned to three groups (n = 10/group) and vaccinated with BMP-15 or GDF-9 peptides conjugated to KLH and adjuvant, or a control of phosphate buffered saline and adjuvant...
May 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29534788/implication-of-transcriptome-profiling-of-spermatozoa-for-stallion-fertility
#18
Yara Suliman, Frank Becker, Klaus Wimmers
Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P ≤0.05, fold change ≥1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P ≤ 0...
March 14, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29530806/the-association-between-gut-microbiome-sex-age-and-body-condition-scores-of-horses-in-maiduguri-and-its-environs
#19
Esther Solomon Mshelia, Lawan Adamu, Yakaka Wakil, Usman Aliyu Turaki, Isa Adamu Gulani, Jasini Musa
The equine gut harbours complex microbial populations which influence physiology, metabolism, nutrition and immune functions, while disruption to the gut microbiota has been linked with conditions such as lameness, diabetes and obesity. Therefore, the present study was conducted to determine the association between microbial dysbiosis, sex, age and body condition scores (BCS) of horses (Equus equus caballus) in Maiduguri and its environs. Forty horses were assessed by convenient sampling, while faecal samples were collected and analyzed to determine the microbiomes in the various age groups with variable BSC in stallions and mares...
March 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29524049/differences-between-horse-selection-based-on-two-forms-of-osteochondrosis-in-fetlock
#20
D Lewczuk, A Bereznowski, M Hecold, M Frąszczak, A Ruść, A Korwin-Kossakowska, J Szyda, S Kamiński
Horses lose potential opportunities because of health problems. Available breeding strategies are not effective enough, probably also because of the different definition used and its genetic usefulness. The aim of the study was to compare the genetic background estimated by the genome-wide association study (GWAS) for osteochondrosis using two different scaling osteochondrosis (OC)/healthy and osteochondrosis dissecans (OCD)/healthy systems for evaluating the disease status of investigated fetlock joints. Two hundred one Warmblood horses trained for performance tests (87 stallions and 114 mares) were phenotyped and genotyped...
May 2018: Journal of Applied Genetics
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