keyword
MENU ▼
Read by QxMD icon Read
search

cyanobacterium

keyword
https://www.readbyqxmd.com/read/28734838/expression-purification-and-characterization-of-sll1981-protein-from-cyanobacterium-synechocystis-sp-pcc6803
#1
Xiaoqin Wang, Guofeng Lei, Xiaoyu Wu, Fei Wang, Chongde Lai, Zhimin Li
The sll1981 protein from cyanobacterium Synechocystis sp. PCC6803 had been reported to exhibit acetolactate synthase (ALS) and L-myo-inositol-1-phosphate synthase (MIPS) activities previously. Based on amino acids sequences alignment, sll1981 protein was postulated to function as α-ketoglutarate decarboxylase (α-KGD), which played important role in completing cyanobacterial tricarboxylic acid (TCA) cycle. However the detailed enzymatic kinetics of sll1981 as ALS, MIPS and α-KGD were not determined yet. In this study, the recombinant sll1981 protein was purified from supernatant of E...
July 19, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28730710/the-molecular-ecology-of-microcystis-sp-blooms-in-the-san-francisco-estuary-delta
#2
Timothy G Otten, Hans W Paerl, Theo W Dreher, Wim J Kimmerer, Alex E Parker
Harmful blooms of the cyanobacterium Microcystis sp. have become increasingly pervasive in the San Francisco Estuary Delta (USA) since the early 2000's and their rise has coincided with substantial decreases in several important fish species. We sought to investigate direct and indirect effects Microcystis blooms may have on the Delta food web. The Microcystis population was tracked for two years at six sites throughout the Delta using quantitative PCR. High-throughput amplicon sequencing and colony PCR sequencing revealed the presence of 10 different strains of Microcystis, including six different microcystin-producing strains...
July 20, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28730560/impairment-of-ntca-gene-revealed-its-role-in-regulating-iron-homeostasis-ros-production-and-cellular-phenotype-under-iron-deficiency-in-cyanobacterium-anabaena-sp-pcc-7120
#3
Manish Singh Kaushik, Meenakshi Srivastava, Anumeha Singh, Arun Kumar Mishra
Iron deficiency ends up into several unavoidable consequences including damaging oxidative stress in cyanobacteria. NtcA is a global nitrogen regulator controls wide range of metabolisms in addition to regulation of nitrogen metabolism. In present communication, NtcA based regulation of iron homeostasis, ROS production and cellular phenotype under iron deficiency in Anabaena 7120 has been investigated. NtcA regulates the concentration dependent iron uptake by controlling the expression of furA gene. NtcA also regulated pigment synthesis and phenotypic alterations in Anabaena 7120...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28729657/spatial-and-temporal-changes-of-parasitic-chytrids-of-cyanobacteria
#4
Mélanie Gerphagnon, Jonathan Colombet, Delphine Latour, Télesphore Sime-Ngando
Parasitism is certainly one of the most important driving biotic factors of cyanobacterial blooms which remains largely understudied. Among these parasites, fungi from the phylum Chytridiomycota (i.e. chytrids) are the only eukaryotic microorganisms infecting cyanobacteria. Here, we address spatiotemporal dynamics of the cyanobacterial host Dolichospermum macrosporum (syn. Anabaena macrospora) and its associated chytrid parasites, Rhizosiphon spp., in an eutrophic lake by studying spatial (vertical, horizontal) and temporal (annual and inter-annual) variations...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28721457/carbon-use-efficiencies-and-allocation-strategies-in-prochlorococcus-marinus-strain-pcc-9511-during-nitrogen-limited-growth
#5
Kristina Felcmanová, Martin Lukeš, Eva Kotabová, Evelyn Lawrenz, Kimberly H Halsey, Ondřej Prášil
We studied cell properties including carbon allocation dynamics in the globally abundant and important cyanobacterium Prochlorococcus marinus strain PCC 9511 grown at three different growth rates in nitrogen-limited continuous cultures. With increasing nitrogen limitation, cellular divinyl chlorophyll a and the functional absorption cross section of Photosystem II decreased, although maximal photosynthetic efficiency of PSII remained unaltered across all N-limited growth rates. Chl-specific gross and net carbon primary production were also invariant with nutrient-limited growth rate, but only 20% of Chl-specific gross carbon primary production was retained in the biomass across all growth rates...
July 18, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28720699/cellular-trade-offs-and-optimal-resource-allocation-during-cyanobacterial-diurnal-growth
#6
Alexandra-M Reimers, Henning Knoop, Alexander Bockmayr, Ralf Steuer
Cyanobacteria are an integral part of Earth's biogeochemical cycles and a promising resource for the synthesis of renewable bioproducts from atmospheric CO2 Growth and metabolism of cyanobacteria are inherently tied to the diurnal rhythm of light availability. As yet, however, insight into the stoichiometric and energetic constraints of cyanobacterial diurnal growth is limited. Here, we develop a computational framework to investigate the optimal allocation of cellular resources during diurnal phototrophic growth using a genome-scale metabolic reconstruction of the cyanobacterium Synechococcus elongatus PCC 7942...
July 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28718632/systematic-approaches-to-efficiently-produce-2-3-butanediol-in-a-marine-cyanobacterium
#7
Nicole E Nozzi, Anna E Case, Austin L Carroll, Shota Atsumi
Cyanobacteria have attracted significant interest as a platform for renewable production of fuel and feedstock chemicals from abundant atmospheric carbon dioxide by way of photosynthesis. While great strides have been made in developing this technology in freshwater cyanobacteria, logistical issues remain in scale-up. Use of the cyanobacterium Synechococcus sp. PCC 7002 (7002) as a chemical production chassis could address a number of these issues given the higher tolerance to salt, light, and heat as well as the fast growth rate of 7002 in comparison to traditional model cyanobacteria such as Synechococcus elongatus PCC 7942 and Synechocystis sp...
July 18, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28712781/pressurized-microcystis-can-help-to-remove-nitrate-from-eutrophic-water
#8
Yingying Huang, Xuechu Chen, Panpan Li, Guiqin Chen, Lin Peng, Liping Pan
The aim of this study was to investigate the feasibility of using harmful cyanobacterium Microcystis to help remove nitrate from eutrophic water. The results showed that after treatment by pressurization at 0.4MPa, Microcystis quickly sank to the bottom. Pressurization did not significantly affect the viability of Microcystis and this cyanobacterium maintained high viability over three days under dark/anoxic conditions. Meanwhile, the amount of dissolved organic carbon (DOC) secreted from living Microcystis cells reached 2...
July 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28710405/the-effect-of-oxygen-concentration-and-temperature-on-nitrogenase-activity-in-the-heterocystous-cyanobacterium-fischerella-sp
#9
Lucas J Stal
Heterocysts are differentiated cells formed by some filamentous, diazotrophic (dinitrogen-fixing) cyanobacteria. The heterocyst is the site of dinitrogen fixation providing the oxygen-sensitive nitrogenase with a low-oxygen environment. The diffusion of air into the heterocyst is a compromise between the maximum influx of dinitrogen gas while oxygen is kept sufficiently low to allow nitrogenase activity. This investigation tested the hypothesis that the heterocyst is capable of controlling the influx of air...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28708310/omics-prediction-of-bioactive-peptides-from-the-edible-cyanobacterium-arthrospira-platensis-proteome
#10
Chaofan Ji, Jing Han, Jingbo Zhang, Jing Hu, Yinghuan Fu, Hang Qi, Yue Sun, Chenxu Yu
BACKGROUND: Bioinformatics approaches are widely used to evaluate the prospects of the novel protein sources in bioactive peptide research. The edible cyanobacteria are considered as the potential protein precursors. However, the abundance of unicellular cyanobacterial proteins is largely unknown and highly dynamic according to the cultivation conditions, which need to be considered in this research field. The objective of this work was to evaluate the protein abundance of Arthrospira platensis, as well as to map the bioactive peptide sequences from the high abundance proteins of the A...
July 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28705982/draft-genome-sequence-of-alkalinema-sp-strain-caciam-70d-a-cyanobacterium-isolated-from-an-amazonian-freshwater-environment
#11
Alex Ranieri Jerônimo Lima, Wendel de Oliveira Castro, Pablo Henrique Gonçalves Moraes, Andrei Santos Siqueira, Délia Cristina Figueira Aguiar, Clayton Pereira Silva de Lima, João Lídio Silva Gonçalves Vianez-Júnior, Márcio Roberto Teixeira Nunes, Leonardo Teixeira Dall'Agnol, Evonnildo Costa Gonçalves
In order to increase the genomic data of cyanobacterial strains isolated in Brazil, we hereby present the draft genome sequence of the Alkalinema sp. strain CACIAM 70d, isolated from an Amazonian freshwater environment. This report describes the first genome available for this genus.
July 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28698278/on-the-origin-of-reverse-transcriptase-using-crispr-cas-systems-and-their-hyperdiverse-enigmatic-spacer-repertoires
#12
Sukrit Silas, Kira S Makarova, Sergey Shmakov, David Páez-Espino, Georg Mohr, Yi Liu, Michelle Davison, Simon Roux, Siddharth R Krishnamurthy, Becky Xu Hua Fu, Loren L Hansen, David Wang, Matthew B Sullivan, Andrew Millard, Martha R Clokie, Devaki Bhaya, Alan M Lambowitz, Nikos C Kyrpides, Eugene V Koonin, Andrew Z Fire
Cas1 integrase is the key enzyme of the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas adaptation module that mediates acquisition of spacers derived from foreign DNA by CRISPR arrays. In diverse bacteria, the cas1 gene is fused (or adjacent) to a gene encoding a reverse transcriptase (RT) related to group II intron RTs. An RT-Cas1 fusion protein has been recently shown to enable acquisition of CRISPR spacers from RNA. Phylogenetic analysis of the CRISPR-associated RTs demonstrates monophyly of the RT-Cas1 fusion, and coevolution of the RT and Cas1 domains...
July 11, 2017: MBio
https://www.readbyqxmd.com/read/28696278/outer-membrane-permeability-of-cyanobacterium-synechocystis-sp-pcc-6803-studies-of-passive-diffusion-of-small-organic-nutrients-reveal-the-absence-of-classical-porins-and-intrinsically-low-permeability
#13
Hikaru Kowata, Saeko Tochigi, Hideyuki Takahashi, Seiji Kojima
The outer membrane of heterotrophic Gram-negative bacteria plays a role of selective permeability barrier that prevents the influx of toxic compounds, while allowing the non-specific passage of small, hydrophilic nutrients through porin channels. Compared with the heterotrophic Gram-negative bacteria, the outer membrane properties of cyanobacteria, the Gram-negative photoautotrophs, are not clearly understood. In this study, using small carbohydrates, amino acids, and inorganic ions as permeation probes, we determined the outer membrane permeability of Synechocystis sp...
July 10, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28692021/additional-families-of-orange-carotenoid-proteins-in-the-photoprotective-system-of-cyanobacteria
#14
Han Bao, Matthew R Melnicki, Emily G Pawlowski, Markus Sutter, Marco Agostoni, Sigal Lechno-Yossef, Fei Cai, Beronda L Montgomery, Cheryl A Kerfeld
The orange carotenoid protein (OCP) is a structurally and functionally modular photoactive protein involved in cyanobacterial photoprotection. Using phylogenomic analysis, we have revealed two new paralogous OCP families, each distributed among taxonomically diverse cyanobacterial genomes. Based on bioinformatic properties and phylogenetic relationships, we named the new families OCP2 and OCPx to distinguish them from the canonical OCP that has been well characterized in Synechocystis, denoted hereafter as OCP1...
July 10, 2017: Nature Plants
https://www.readbyqxmd.com/read/28687036/photosynthetic-poly-%C3%AE-hydroxybutyrate-accumulation-in-unicellular-cyanobacterium-synechocystis-sp-pcc-6714
#15
Donya Kamravamanesh, Stefan Pflügl, Winfried Nischkauer, Andreas Limbeck, Maximilian Lackner, Christoph Herwig
Poly-β-hydroxybutyrate (PHB) production from CO2 has the potential to reduce the production cost of this biodegradable polyesters, and also to make the material more sustainable compared to utilization of sugar feedstocks. In this study the unicellular cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential organism for production of PHB. Synechocystis sp. PCC 6714 was studied under various cultivation conditions and nutritional limitations. Combined effects of nitrogen and phosphorus deficiency led to highest PHB accumulation under photoautotrophic conditions...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28681162/copper-based-aquatic-algaecide-adsorption-and-accumulation-kinetics-influence-of-exposure-concentration-and-duration-for-controlling-the-cyanobacterium-lyngbya-wollei
#16
West M Bishop, Clayton L Lynch, Ben E Willis, W Gregory Cope
Filamentous mat-forming cyanobacteria are increasingly impairing uses of freshwater resources. To effectively manage, a better understanding of control measures is needed. Copper (Cu)-based algaecide formulations are often applied to reactively control nuisance cyanobacterial blooms. This laboratory research assessed typical field exposure scenarios for the ability of Cu to partition to, and accumulate in Lyngbya wollei. Exposure factors (Cu concentration × duration) of 4, 8, 16, 24, 32 h were tested across three aqueous Cu concentrations (1, 2, 4 ppm)...
July 5, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28675820/grazing-of-nuclearia-thermophila-and-nuclearia-delicatula-nucleariidae-opisthokonta-on-the-toxic-cyanobacterium-planktothrix-rubescens
#17
Sebastian Dirren, Gianna Pitsch, Marisa O D Silva, Thomas Posch
During the last decades, the planktonic cyanobacterium Planktothrix rubescens became a dominant primary producer in many deep pre-alpine lakes. While altered physiochemical conditions due to lake warming seem to favour this cyanobacterial species, its dominance is partly attributed to factors conferring grazing resistance. The rigid structure of the cyanobacterial filaments and toxic secondary metabolites (e.g. microcystins) protect against diverse grazers. Nonetheless, species of the protistan genus Nuclearia (Nucleariidae, Opisthokonta) are able to overcome this grazing protection...
June 8, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/28673519/efficient-assimilation-of-cyanobacterial-nitrogen-by-water-hyacinth
#18
Hongjie Qin, Zhiyong Zhang, Minhui Liu, Yan Wang, Xuezheng Wen, Shaohua Yan, Yingying Zhang, Haiqin Liu
A (15)N labeling technique was used to study nitrogen transfer from cyanobacterium Microcystis aeruginosa to water hyacinth. (15)N atom abundance in M. aeruginosa peaked (15.52%) after cultivation in (15)N-labeled medium for 3weeks. Over 87% of algal nitrogen was transferred into water hyacinth after the 4-week co-cultivation period. The nitrogen quickly super-accumulated in the water hyacinth roots, and the labeled nitrogen was re-distributed to different organs (i.e., roots, stalks, and leaves). This study provides a new strategy for further research on cyanobacterial bloom control, nitrogen migration, and nitrogen cycle in eutrophic waters...
October 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28670308/growth-characteristics-of-an-estuarine-heterocystous-cyanobacterium
#19
Pablo Guimarães, João S Yunes, Mariana Silvia Cretoiu, Lucas J Stal
A new estuarine filamentous heterocystous cyanobacterium was isolated from intertidal sediment of the Lagoa dos Patos estuary (Brazil). The isolate may represent a new genus related to Cylindrospermopsis. While the latter is planktonic, contains gas vesicles, and is toxic, the newly isolated strain is benthic and does not contain gas vesicles. It is not known whether the new strain is toxic. It grows equally well in freshwater, brackish and full salinity growth media, in the absence of inorganic or organic combined nitrogen, with a growth rate 0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28668777/translating-divergent-environmental-stresses-into-a-common-proteome-response-through-the-histidine-kinase-33-hik33-in-a-model-cyanobacterium
#20
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, Yingchun Wang
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly like that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival...
July 2017: Molecular & Cellular Proteomics: MCP
keyword
keyword
59427
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"