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https://www.readbyqxmd.com/read/27926916/elevated-temperature-and-allelopathy-impact-coral-recruitment
#1
Raphael Ritson-Williams, Cliff Ross, Valerie J Paul
As climate change continues to alter seawater temperature and chemistry on a global scale, coral reefs show multiple signs of degradation. One natural process that could facilitate the recovery of reef ecosystems is coral recruitment, which can be influenced by the benthic organisms in a local habitat. We experimentally tested both a global stressor (increased seawater temperature) and a local stressor (exposure to microcolin A, a natural product from a common marine benthic cyanobacterium) to determine how these stressors impacted coral larval sublethal stress, survival and settlement...
2016: PloS One
https://www.readbyqxmd.com/read/27924128/exopolysaccharide-production-in-anabaena-sp-pcc-7120-under-different-cacl2-regimes
#2
Savita Singh, Ekta Verma, Niveshika, Balkrishna Tiwari, Arun Kumar Mishra
Influence of various levels of CaCl2 (0, 1, 10 and 100 mM) on exopolysaccharide production has been investigated in the cyanobacterium Anabaena 7120. At the concentration of 1 mM CaCl2, growth was found to be stimulatory while 100 mM was sub lethal for the cyanobacterial cells. Estimation of EPS content revealed that EPS production depends on the concentration of calcium ions in the immediate environment with maximum being at10 mM CaCl2. A possible involvement of alr2882 gene in the process of EPS production was also revealed through qRT-PCR...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27920300/the-role-of-the-nifb1-and-nifb2-promoters-in-cell-type-specific-expression-of-two-mo-nitrogenases-in-the-cyanobacterium-anabaena-variabilis-atcc-29413
#3
Susan Vernon, Brenda S Pratte, Teresa Thiel
: Anabaena variabilis ATCC 29413 has one Mo-nitrogenase that is made under oxic growth conditions in specialized cells called heterocysts and a second Mo-nitrogenase that is made only under anoxic conditions in vegetative cells. The two large nif gene clusters responsible for these two nitrogenases are under the control of the promoter of the first gene, nifB1 or nifB2, in the operon. Despite differences in expression patterns of nifB1 and nifB2 related to oxygen and cell type, the regions upstream of their transcription start sites (tss) show striking homology, including three highly conserved sequences (CS)...
December 5, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27914646/in-the-eye-of-the-beholder-assessing-the-water-quality-of-shoreline-parks-around-the-island-of-montreal-through-citizen-science
#4
D Lévesque, A Cattaneo, G Deschamps, C Hudon
As a part of the FreshWater Watch project aiming to promote volunteers' water monitoring in 25 cities around the world, St. Lawrence River water quality was characterized at 28 public shoreline parks around Montreal Island, Quebec, Canada. This involved training of 69 citizen scientists by researchers of the Université de Montréal in five one-day sessions. Shoreline sampling yielded 174 data points over three summers (May 2013 to November 2015). Water turbidity, nitrate and phosphate concentrations were measured in situ, together with the thickness and type of beach-cast vegetation, and the relative abundance of different types of beach litter...
November 30, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27911809/unique-attributes-of-cyanobacterial-metabolism-revealed-by-improved-genome-scale-metabolic-modeling-and-essential-gene-analysis
#5
Jared T Broddrick, Benjamin E Rubin, David G Welkie, Niu Du, Nathan Mih, Spencer Diamond, Jenny J Lee, Susan S Golden, Bernhard O Palsson
The model cyanobacterium, Synechococcus elongatus PCC 7942, is a genetically tractable obligate phototroph that is being developed for the bioproduction of high-value chemicals. Genome-scale models (GEMs) have been successfully used to assess and engineer cellular metabolism; however, GEMs of phototrophic metabolism have been limited by the lack of experimental datasets for model validation and the challenges of incorporating photon uptake. Here, we develop a GEM of metabolism in S. elongatus using random barcode transposon site sequencing (RB-TnSeq) essential gene and physiological data specific to photoautotrophic metabolism...
December 1, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27911807/enhanced-limonene-production-in-cyanobacteria-reveals-photosynthesis-limitations
#6
Xin Wang, Wei Liu, Changpeng Xin, Yi Zheng, Yanbing Cheng, Su Sun, Runze Li, Xin-Guang Zhu, Susie Y Dai, Peter M Rentzepis, Joshua S Yuan
Terpenes are the major secondary metabolites produced by plants, and have diverse industrial applications as pharmaceuticals, fragrance, solvents, and biofuels. Cyanobacteria are equipped with efficient carbon fixation mechanism, and are ideal cell factories to produce various fuel and chemical products. Past efforts to produce terpenes in photosynthetic organisms have gained only limited success. Here we engineered the cyanobacterium Synechococcus elongatus PCC 7942 to efficiently produce limonene through modeling guided study...
November 23, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27907245/biodegradation-of-the-aminopolyphosphonate-dtpmp-by-the-cyanobacterium-anabaena-variabilis-proceeds-via-a-c-p-lyase-independent-pathway
#7
Damian Drzyzga, Giuseppe Forlani, Jochen Vermander, Paweł Kafarski, Jacek Lipok
Cyanobacteria, the only prokaryotes capable of oxygenic photosynthesis, play a major role in carbon, nitrogen and phosphorus global cycling. Under conditions of increased P availability and nutrient loading, some cyanobacteria are capable of blooming, rapidly multiplying and possibly altering the ecological structure of the ecosystem. Because of their ability of using non-conventional P sources, these microalgae can be used for bioremediation purposes. Under this perspective, the metabolization of the polyphosphonate diethylenetriaminepenta(methylenephosphonic) acid (DTPMP) by the strain CCALA 007 of Anabaena variabilis was investigated using (31) P NMR analysis...
December 1, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27904937/separation-of-cyclic-lipopeptide-puwainaphycins-from-cyanobacteria-by-countercurrent-chromatography-combined-with-polymeric-resins-and-hplc
#8
José Cheel, Petra Urajová, Jan Hájek, Pavel Hrouzek, Marek Kuzma, Elodie Bouju, Karine Faure, Jiří Kopecký
Puwainaphycins are a recently described group of β-amino fatty acid cyclic lipopeptides of cyanobacterial origin that possess interesting biological activities. Therefore, the development of an efficient method for their isolation from natural sources is necessary. Following the consecutive adsorption of the crude extract on Amberlite XAD-16 and XAD-7 resins, countercurrent chromatography (CCC) was applied to separate seven puwainaphycin variants from a soil cyanobacterium (Cylindrospermum alatosporum CCALA 988)...
November 30, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27902845/cloning-and-heterologous-expression-of-chlorophyll-a-synthase-in-rhodobacter-sphaeroides
#9
Emre M Ipekoğlu, Koray Göçmen, Mehmet T Öz, Muazzez Gürgan, Meral Yücel
Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption spectrum within 400-700 nm...
November 30, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27902432/self-replicating-shuttle-vectors-based-on-pans-a-small-endogenous-plasmid-of-the-unicellular-cyanobacterium-synechococcus-elongatus-pcc-7942
#10
You Chen, Arnaud Taton, Michaela Go, Ross E London, Lindsey M Pieper, Susan S Golden, James W Golden
To facilitate development of synthetic biology tools for genetic engineering of cyanobacterial strains, we constructed pANS-derived self-replicating shuttle vectors that are based on the minimal replication element of the Synechococcus elongatus strain PCC 7942 plasmid pANS. To remove the possibility of homologous recombination events between the shuttle plasmids and the native pANS plasmid, the endogenous pANS was cured through plasmid incompatibility-mediated spontaneous loss. A heterologous toxin-antitoxin cassette was incorporated into the shuttle vectors for stable plasmid maintenance in the absence of antibiotic selection...
October 14, 2016: Microbiology
https://www.readbyqxmd.com/read/27902306/description-of-gloeomargarita-lithophora-gen-nov-sp-nov-a-thylakoid-bearing-basal-branching-cyanobacterium-with-intracellular-carbonates-and-proposal-for-gloeomargaritales-ord-nov
#11
David Moreira, Rosaluz Tavera, Karim Benzerara, Fériel Skouri-Panet, Estelle Couradeau, Emmanuelle Gérard, Céline Loussert Fonta, Eberto Novelo, Yvan Zivanovic, Purificación López-García
A unicellular cyanobacterium, strain Alchichica-D10, was isolated from microbialites of the alkaline Lake Alchichica, Mexico. The cells were short rods (3.9 ± 0.6 μm in length and 1.1 ± 0.1 μm in width) forming biofilms of intense emerald green color. They exhibited red autofluorescence under UV light excitation. UV-visible absorption spectra revealed that they contain chlorophyll a and phycocyanin, and electron microscopy showed the presence of thylakoids. The strain grew within a temperature range of 15-30 °C...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27899536/a-comprehensively-curated-genome-scale-two-cell-model-for-the-cyanobacterium-anabaena-sp-pcc-7120
#12
David Malatinszky, Ralf Steuer, Patrik R Jones
Anabaena sp. PCC 7120 is a nitrogen-fixing filamentous cyanobacterium. Under nitrogen limiting conditions, a fraction of the vegetative cells in each filament terminally differentiate to non-growing heterocysts. Heterocysts are metabolically and structurally specialized to enable O2-sensitive nitrogen fixation. The functionality of the filament, as an association of vegetative cells and heterocysts, is postulated to depend on metabolic exchange of electrons, carbon and fixed nitrogen. In the present work, we compile and evaluate a comprehensive curated stoichiometric model of this two-cell system, with the objective function based on the growth of the filament under diazotrophic conditions...
November 29, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27895626/a-proposal-for-formation-of-archaean-stromatolites-before-the-advent-of-oxygenic-photosynthesis
#13
John F Allen
Stromatolites are solid, laminar structures of biological origin. Living examples are sparsely distributed and formed by cyanobacteria, which are oxygenic phototrophs. However, stromatolites were abundant between 3.4 and 2.4 Gyr, prior to the advent of cyanobacteria and oxygenic photosynthesis. Here I propose that many Archaean stromatolites were seeded at points of efflux of hydrogen sulfide from hydrothermal fields into shallow water, while their laminar composition arose from alternating modes of strictly anoxygenic photosynthetic metabolism...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27891682/robust-min-system-oscillation-in-the-presence-of-internal-photosynthetic-membranes-in-cyanobacteria
#14
Joshua S MacCready, Jory Schossau, Katherine W Osteryoung, Daniel C Ducat
The oscillatory Min system of Escherichia coli defines the cell division plane by regulating the site of FtsZ-ring formation and represents one of the best-understood examples of emergent protein self-organization in nature. The oscillatory patterns of the Min-system proteins MinC, MinD and MinE (MinCDE) are strongly dependent on the geometry of membranes they bind. Complex internal membranes within cyanobacteria could disrupt this self-organization by sterically occluding or sequestering MinCDE from the plasma membrane...
November 5, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27888364/in-vitro-reconstitution-of-the-cyclosporine-specific-p450-hydroxylases-using-heterologous-redox-partner-proteins
#15
Yue Sun, Li Ma, Dongfei Han, Lei Du, Fengxia Qi, Wei Zhang, Jingran Sun, Shan Huang, Eung-Soo Kim, Shengying Li
The cytochrome P450 enzymes (CYPs) CYP-sb21 from Sebekia benihana and CYP-pa1 from Pseudonocardia autotrophica are able to hydroxylate the immunosuppressant cyclosporin A (CsA) in a regioselective manner, giving rise to the production of two hair-stimulating agents (with dramatically attenuated immunosuppressant activity), γ-hydroxy-N-methyl-L-Leu4-CsA (CsA-4-OH) and γ-hydroxy-N-methyl-L-Leu9-CsA (CsA-9-OH). Recently, the in vitro activity of CYP-sb21 was identified using several surrogate redox partner proteins...
November 25, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27886582/crude-extract-of-cyanobacteria-radiocystis-fernandoi-strain-r28-induces-liver-impairments-in-fish
#16
M G Paulino, D Tavares, F Bieczynski, P G Pedrão, N E S Souza, M M Sakuragui, C M Luquet, A P Terezan, J B Fernandes, A Giani, M N Fernandes
Radiocystis fernandoi R28 strain is a cyanobacterium which produces mostly the RR and YR microcystin variants (MC-RR and MC-YR, respectively). The effects of crude extract of the R. fernandoi strain R28 were evaluated on the protein phosphatases and on the structure and ultrastructure of the liver of the Neotropical fish, Hoplias malabaricus, after acute and subchronic exposure. Concomitantly, the accumulation of the majority of MCs was determined in the liver and muscle. The fish were exposed to 120.60 MC-RR+MC-LR kg-fish(-1) (=100μg MC-LReq kg-fish(-1)) for 12 and 96h (one single dose, acute exposure) and 30days (one similar dose every 72h, subchronic exposure)...
November 17, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27883080/construction-of-a-miniaturized-chromatic-acclimation-sensor-from-cyanobacteria-with-reversed-response-to-a-light-signal
#17
Mitsuharu Nakajima, Stefano Ferri, Matthias Rögner, Koji Sode
Cyanobacteria harbor unique photoreceptors, designated as cyanobacteriochromes (CBCRs). In this study, we attempted to engineer the chromatic acclimation sensor CcaS, a CBCR derived from the cyanobacterium Synechocystis sp. PCC 6803. The wild-type CcaS induces gene expression under green light illumination and represses it under red light illumination. We focused on the domain structure of CcaS, which consists of an N-terminal transmembrane helix; a GAF domain, which serves as the sensor domain; a linker region (L1); two PAS domains; a second linker region (L2); and a C-terminal histidine kinase (HK) domain...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27878952/newly-isolated-nodularia-phage-influences-cyanobacterial-community-dynamics
#18
S E Coloma, A Dienstbier, D H Bamford, K Sivonen, E Roine, T Hiltunen
Cyanophages, i.e. viruses infecting cyanobacteria, are a key component driving cyanobacterial community dynamics both ecologically and evolutionarily. In addition to reducing biomass and influencing the genetic diversity of their host populations, they can also have a wider community-level impact due to the release of nutrients by phage-induced cell lysis. In this study, we isolated and characterized a new cyanophage, a siphophage designated as vB_NpeS-2AV2, capable of infecting the filamentous nitrogen fixing cyanobacterium Nodularia sp...
November 23, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27869542/treatment-challenge-of-a-non-toxic-cyanobacterium-romeria-elegans-bloom-event-in-a-south-australian-wastewater-treatment-plant-a-case-study
#19
Radisti A Praptiwi, Carlos J Pestana, Emma T Sawade, Nick Swain, Gretchen Schroeder, Gayle Newcombe
A bloom of the non-toxic cyanobacterium Romeria elegans in waste stabilisation ponds within Angaston waste water treatment plant (WWTP) has posed an unprecedented treatment challenge for the local water utility. The water from the stabilisation ponds is chlorinated for safety prior to reuse on nearby farmland. Cyanobacteria concentrations of approximately 1.2×10(6) cells mL(-1) increased the chlorine demand dramatically. Operators continuously increased the disinfectant dose up to 50 mg L(-1) to achieve operational guideline values for combined chlorine (0...
November 21, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27868089/adaptive-evolution-of-phosphorus-metabolism-in-prochlorococcus
#20
John R Casey, Adil Mardinoglu, Jens Nielsen, David M Karl
Inorganic phosphorus is scarce in the eastern Mediterranean Sea, where the high-light-adapted ecotype HLI of the marine picocyanobacterium Prochlorococcus marinus thrives. Physiological and regulatory control of phosphorus acquisition and partitioning has been observed in HLI both in culture and in the field; however, the optimization of phosphorus metabolism and associated gains for its phosphorus-limited-growth (PLG) phenotype have not been studied. Here, we reconstructed a genome-scale metabolic network of the HLI axenic strain MED4 (iJC568), consisting of 568 metabolic genes in relation to 794 reactions involving 680 metabolites distributed in 6 subcellular locations...
November 2016: MSystems
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