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Extended spectrum betalactamase

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https://www.readbyqxmd.com/read/27902381/detection-of-escherichia-coli-st131-complex-st705-and-klebsiella-pneumoniae-st15-among-fecal-carriage-of-extended-spectrum-betalactamase-and-carbapenemase-producing-enterobacteriaceae
#1
Esther Rios, Maria Carmen López, Iciar Rodríguez-Avial, Esther Culebras, Juan Jose Picazo
The objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and carbapenemases-producing Enterobacteriaceae (CPE) in non-selected hospitalized and non-hospitalized patients from the same geographic area of Madrid. Among the 501 patients enrolled, 43 (8.6%) carried ESBL-E and 8 (1.6%) patients exhibited CPE. The main intestinal colonizer among ESBL-E was CTX-M-producing Escherichia coli isolates in both settings (community and hospital)...
November 28, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27798637/the-technical-and-biological-reproducibility-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-based-typing-employment-of-bioinformatics-in-a-multicenter-study
#2
Michael Oberle, Nadia Wohlwend, Daniel Jonas, Florian P Maurer, Geraldine Jost, Sarah Tschudin-Sutter, Katleen Vranckx, Adrian Egli
BACKGROUND: The technical, biological, and inter-center reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI TOF MS) typing data has not yet been explored. The aim of this study is to compare typing data from multiple centers employing bioinformatics using bacterial strains from two past outbreaks and non-related strains. MATERIAL/METHODS: Participants received twelve extended spectrum betalactamase-producing E. coli isolates and followed the same standard operating procedure (SOP) including a full-protein extraction protocol...
2016: PloS One
https://www.readbyqxmd.com/read/27581926/mossambicus-tilapia-oreochromis-mossambicus-collected-from-water-bodies-impacted-by-urban-waste-carries-extended-spectrum-beta-lactamases-and-integron-bearing-gut-bacteria
#3
Nachiket P Marathe, Swapnil S Gaikwad, Ankita A Vaishampayan, Mandar H Rasane, Yogesh S Shouche, Wasudev N Gade
Oreochromis mossambicus (Peters 1852) (Tilapia) is one of the most consumed fish globally. Tilapia thrives well in environments polluted by urban waste, which invariably contain antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Thus, Tilapia surviving in such polluted environments may serve as a potential source for dissemination of ARGs. To investigate this, we isolated bacterial strains from gut of Tilapia found in polluted rivers and lakes near Pune, India, and studied the prevalence of resistance genes by molecular methods...
September 2016: Journal of Biosciences
https://www.readbyqxmd.com/read/27494134/esbl-detection-comparison-of-a-commercially-available-chromogenic-test-for-third-generation-cephalosporine-resistance-and-automated-susceptibility-testing-in-enterobactericeae
#4
Mohamed Ramadan El-Jade, Marijo Parcina, Ricarda Maria Schmithausen, Christoph Stein, Alina Meilaender, Achim Hoerauf, Ernst Molitor, Isabelle Bekeredjian-Ding
Rapid detection and reporting of third generation cephalosporine resistance (3GC-R) and of extended spectrum betalactamases in Enterobacteriaceae (ESBL-E) is a diagnostic and therapeutic priority to avoid inefficacy of the initial antibiotic regimen. In this study we evaluated a commercially available chromogenic screen for 3GC-R as a predictive and/or confirmatory test for ESBL and AmpC activity in clinical and veterinary Enterobacteriaceae isolates. The test was highly reliable in the prediction of cefotaxime and cefpodoxime resistance, but there was no correlation with ceftazidime and piperacillin/tazobactam minimal inhibitory concentrations...
2016: PloS One
https://www.readbyqxmd.com/read/27373765/decline-of-multidrug-resistant-gram-negative-infections-with-the-routine-use-of-a-multiple-decontamination-regimen-in-icu
#5
Christophe Camus, Elise Sauvadet, Aude Tavenard, Caroline Piau, Fabrice Uhel, Pierre Bouju, Julien Letheulle, Gilles Dollo, Arnaud Gacouin, Sylvain Lavoué, Yves Le Tulzo
OBJECTIVES: We have shown that the routine use of a multiple decontamination regimen with oropharyngeal and digestive polymyxin/tobramycin/amphotericin B plus mupirocin/chlorhexidine in intubated patients reduced all-cause acquired infections (AIs) in the intensive care unit (ICU). We now assessed the long-term impact of this strategy on AIs involving multidrug-resistant aerobic Gram negative bacilli (AGNB) and acquired episodes of extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae rectal carriage...
September 2016: Journal of Infection
https://www.readbyqxmd.com/read/27223840/travel-to-asia-and-traveller-s-diarrhoea-with-antibiotic-treatment-are-independent-risk-factors-for-acquiring-ciprofloxacin-resistant-and-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-a-prospective-cohort-study
#6
E A Reuland, G J B Sonder, I Stolte, N Al Naiemi, A Koek, G B Linde, T J W van de Laar, C M J E Vandenbroucke-Grauls, A P van Dam
Travel to (sub)tropical countries is a well-known risk factor for acquiring resistant bacterial strains, which is especially of significance for travellers from countries with low resistance rates. In this study we investigated the rate of and risk factors for travel-related acquisition of extended spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E), ciprofloxacin-resistant Enterobacteriaceae (CIPR-E) and carbapenem-resistant Enterobacteriaceae. Data before and after travel were collected from 445 participants...
August 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/26815433/epidemiological-and-clinical-features-for-cefepime-heteroresistant-escherichia-coli-infections-in-southwest-china
#7
W Ma, J Sun, S Yang, L Zhang
Phenotypic heteroresistance (PHR) is common in a weight of microbes and plays an important role in the evolution of antibiotic resistance. However, PHR to cefepime (FEP-PHR) among invasive Escherichia coli (E. coli) has not been reported. This study aimed to report the characteristics of invasive E. coli with FEP-PHR traits and further to investigate the predisposing factors for its acquisition. A retrospective case-control study was conducted in a teaching hospital in Southwest China. A total of 319 successive and non-duplicate E...
April 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/26766415/bloodstream-infections-after-solid-organ-transplantation
#8
Antonios Kritikos, Oriol Manuel
Solid-organ transplantation (SOT) has become the preferred strategy to treat a number of end-stage organ disease, because a continuous improvement in survival and quality of life. While preventive strategies has decreased the risk for classical opportunistic infections (such as viral, fungal and parasite infections), bacterial infections, and particularly bloodstream infections (BSIs) remain the most common and life-threatening complications in SOT recipients. The source of BSI after transplant depends on the type of transplantation, being urinary tract infection, pneumonia, and intraabdominal infections the most common infections occurring after kidney, lung and liver transplantation, respectively...
April 2, 2016: Virulence
https://www.readbyqxmd.com/read/26707079/association-of-risk-factors-antimicrobial-resistance-trends-and-occurrence-of-blatem-bla-shv-and-blactx-m-in-escherichia-coli-causing-bacteremia
#9
Richa Sinha, Sphurti Kamath, Suchitra M Shenoy
PURPOSE: Escherichia coli are the most frequent cause of gram negative bloodstream infection. This study was done to evaluate the association of risk factors, antimicrobial susceptibility pattern and detection of TEM, SHV and CTX M genes in the extended spectrum beta lactamase (ESBL) producing E.coli. MATERIALS AND METHODS: 11,133 blood samples were processed in BacT/Alert bottles. Bacteria grown were identified and antibiotic susceptibility patterns were studied using VITEK2 system...
2016: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/26505186/-multidrug-resistant-organisms-mdro-in-rehabilitation-clinics-in-the-rhine-main-district-germany-2014-prevalence-and-risk-factors
#10
MULTICENTER STUDY
U Heudorf, D Färber, D Mischler, M Schade, C Zinn, C Cuny, D Nillius, M Herrmann
BACKGROUND: While a limited number of studies have investigated the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in medical rehabilitation institutions, almost no data on the colonization of rehabilitation patients with multiresistant gram-negative rods is available. Here we report on a large multicenter study on the prevalence of MRSA and multiresistant pathogens in rehabilitation institutions in the Rhine-Main area in 2014. MATERIALS AND METHODS: Altogether, 21 rehabilitation hospitals participated...
October 2015: Die Rehabilitation
https://www.readbyqxmd.com/read/26213583/simultaneous-gut-colonisation-and-infection-by-esbl-producing-escherichia-coli-in-hospitalised-patients
#11
Johny Asir, Shashikala Nair, Sheela Devi, Kenchappa Prashanth, Rajagopalan Saranathan, Reba Kanungo
BACKGROUND: Extended spectrum betalactamase (ESBL)-producing organisms are a major cause of hospital-acquired infections. ESBL-producing Escherichia coli (E. coli) have been recovered from the hospital environment. These drug-resistant organisms have also been found to be present in humans as commensals. The present investigation intended to isolate ESBL-producing E. coli from the gut of already infected patients; to date, only a few studies have shown evidence of the gut microflora as a major source of infection...
2015: Australasian Medical Journal
https://www.readbyqxmd.com/read/26008659/in-vitro-efficacy-of-meropenem-colistin-and-tigecycline-against-the-extended-spectrum-beta-lactamase-producing-gram-negative-bacilli
#12
COMPARATIVE STUDY
Maria Mushtaq Gill, Javaid Usman, Afreenish Hassan, Fatima Kaleem, Rabia Anjum
OBJECTIVE: To compare the in vitro efficacy of meropenem, colistin and tigecycline against extended spectrum Betalactamase producing Gram negative bacilli by minimal inhibitory concentration. STUDY DESIGN: Cross-sectional descriptive study. PLACE AND DURATION OF STUDY: Department of Microbiology, Army Medical College, National University of Sciences and Technology, Rawalpindi, from June to December 2010. METHODOLOGY: Routine clinical specimens were subjected to standard microbiological procedures and the isolates were identified to species level...
May 2015: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/25890291/simple-multiplex-pcr-assays-to-detect-common-pathogens-and-associated-genes-encoding-for-acquired-extended-spectrum-betalactamases-esbl-or-carbapenemases-from-surgical-site-specimens-in-vietnam
#13
Ngo Tat Trung, Tran Thi Thu Hien, Tran Thi Thanh Huyen, Dao Thanh Quyen, Mai Thanh Binh, Phan Quoc Hoan, Christian G Meyer, Thirumalaisamy P Velavan, Le Huu Song
UNLABELLED: Surgical site infection (SSI) is common in Vietnamese post-operative patients. It contributes to increased morbidity, mortality, hospitalization time and health care expenditure. Bacterial culture is considered the gold standard procedure to identify SSI pathogens and antibiotic resistant properties; however, it can detect microbes that can readily grow and is time-consuming. We propose optimized multiplex PCR assays to diagnose the most relevant microbes and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from Vietnamese patients with SSI in a hospital setting in Hanoi...
2015: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/25676738/impact-of-antibiotic-resistance-on-the-development-of-recurrent-and-relapsing-symptomatic-urinary-tract-infection-in-kidney-recipients
#14
M Bodro, G Sanclemente, I Lipperheide, M Allali, F Marco, J Bosch, F Cofan, M J Ricart, N Esforzado, F Oppenheimer, A Moreno, C Cervera
We sought to determine the frequency, risk factors, and clinical impact of recurrent urinary tract infections (UTI) in kidney transplant recipients. Of 867 patients who received a kidney transplant between 2003 and 2010, 174 (20%) presented at least one episode of UTI. Fifty-five patients presented a recurrent UTI (32%) and 78% of them could be also considered relapsing episodes. Recurrent UTI was caused by extended-spectrum betalactamase (ESBL)-producing Klebsiella pneumoniae (31%), followed by non-ESBL producing Escherichia coli (15%), multidrug-resistant (MDR) Pseudomonas aeruginosa (14%), and ESBL-producing E...
April 2015: American Journal of Transplantation
https://www.readbyqxmd.com/read/25613287/antimicrobials-increase-travelers-risk-of-colonization-by-extended-spectrum-betalactamase-producing-enterobacteriaceae
#15
Anu Kantele, Tinja Lääveri, Sointu Mero, Katri Vilkman, Sari H Pakkanen, Jukka Ollgren, Jenni Antikainen, Juha Kirveskari
BACKGROUND: More than 300 million travelers visit regions with poor hygiene annually. A significant percentage of them become colonized by resistant intestinal bacteria such as extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) and may transmit the strains to others and to medical care settings when they return home. Despite the threats to global healthcare caused by an upsurge in antimicrobial resistance, no effort has been centered on prevention of colonization while traveling...
March 15, 2015: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/25310547/-ventilator-associated-pneumonia-confection-of-a-strategy-of-prophylaxis-and-management-based-on-the-analysis-of-epidemiology
#16
Edgard do Carmo Neto, Paulo César de Souza, Frederico Azevedo, Marcelo Elisio Lugarinho
BACKGROUND AND OBJECTIVES: Variable magnitude of impact on the outcomes of the critically ill patients has been credited to ventilation-associated pneumonia, in terms of mortality, length of hospital stay and mechanic ventilation days. Three objectives have been defined in this study: mortality and incidence of ventilation-associated pneumonia before and after the implantation of a prophylaxis protocol (primary objectives); microbiologic mapping (secondary objective) as an instrument to optimize therapy...
December 2006: Revista Brasileira de Terapia Intensiva
https://www.readbyqxmd.com/read/25184764/experimental-model-for-treatment-of-extended-spectrum-betalactamase-producing-klebsiella-pneumoniae
#17
Paula Virginia Michelon Toledo, Felipe Francisco Tuon, Larissa Bail, Francine Manente, Polliane Arruda, Ayrton Alves Aranha-Junior
BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums ranging from 1.5x109 colony-forming units per milliliter (CFU/mL) to 2.0x1010 CFU/mL were administered by peritoneal injection in adults Wistar rats...
July 2014: Arquivos Brasileiros de Cirurgia Digestiva: ABCD, Brazilian Archives of Digestive Surgery
https://www.readbyqxmd.com/read/24968032/-risk-factors-associated-with-the-isolation-of-extended-spectrum-betalactamases-producing-escherichia-coli-or-klebsiella-pneumoniae-in-a-tertiary-care-hospital-in-colombia
#18
COMPARATIVE STUDY
Adriana Jiménez, Alejandra Alvarado, Felipe Gómez, Germán Carrero, Claudia Fajardo
INTRODUCTION: Extended-spectrum beta-lactamases (ESBL) are an emerging resistance phenomenon with particular incidence in Latin America. In Colombia there is very little information regarding the risk factors associated with its acquisition. OBJECTIVE: To determine the risk factors that are associated with infection or colonization by Escherichia coli or Klebsiella pneumoniae producing ESBL in patients older than 18 years. MATERIALS AND METHODS: A case-control study, ratio 1:1, in patients with an isolate from any sample of E...
April 2014: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/24834305/extended-spectrum-betalactamase-producing-enteroaggregative-escherichia-coli-from-young-children-in-iran
#19
Hakimeh Khoshvaght, Fakhri Haghi, Habib Zeighami
AIM: The aim of this study was to investigate the frequency of betalactamase producing EAEC isolates among young children with diarrhea in Zanjan, Iran. BACKGROUND: Entero aggregative Escherichia coli (EAEC) is an emerging enteric pathogen associated with acute and persistent diarrhea and the evolution and spread of acquired extended spectrum betalactamases (ESBLs) among these strains has become a serious problem in the management of infectious diseases in developing countries...
2014: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/24577973/urinary-tract-infections-caused-by-extended-spectrum-betalactamase-producing-bacteria-in-children-a-matched-casecontrol-study
#20
John Dotis, Nikoleta Printza, Alexandra Marneri, Dimos Gidaris, Fotios Papachristou
Extended-spectrum beta-lactamase (ESBL)-producing pathogens are emerging as a cause of urinary tract infections (UTI) worldwide. In this matched-case control study, clinical characteristics and associated risk factors for ESBL UTI were evaluated. In a total of 463 positive urine cultures, 48 (10.4%) (from 39 patients, 23 boys) were phenotypically ESBL-producing bacteria. The most frequently isolated microorganism was , followed by Klebsiella spp. and Enterobacter cloacae. Children with ESBL UTI (n=39) were on prophylaxis more (21% vs...
November 2013: Turkish Journal of Pediatrics
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