keyword
MENU ▼
Read by QxMD icon Read
search

Luminex

keyword
https://www.readbyqxmd.com/read/29228581/a-multiplex-liquid-chip-assay-based-on-luminex-xmap-technology-for-simultaneous-detection-of-six-common-respiratory-viruses
#1
Yong Yan, Jian-Yong Luo, Yin Chen, Heng-Hui Wang, Guo-Ying Zhu, Pei-Yan He, Jin-Lei Guo, Yong-Liang Lei, Zhong-Wen Chen
We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus to China, Middle East Respiratory Syndrome coronavirus (MERS-CoV). Performance of rMLA was evaluated by comparing with real-time RT-PCR...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29227832/functional-characterization-and-phenotypic-monitoring-of-human-hematopoietic-stem-cell-expansion-and-differentiation-of-monocytes-and-macrophages-by-whole-cell-mass-spectrometry
#2
Guido Vogel, Aline Cuénod, Roxanne Mouchet, André Strauss, Claudia Daubenberger, Valentin Pflüger, Damien Portevin
The different facets of macrophages allow them to play distinct roles in tissue homeostasis, tissue repair and in response to infections. Individuals displaying dysregulated macrophage functions are proposed to be prone to inflammatory disorders or infections. However, this being a cause or a consequence of the pathology remains often unclear. In this context, we isolated and expanded CD34+ HSCs from healthy blood donors and derived them into CD14+ myeloid progenitors which were further enriched and differentiated into macrophages...
November 22, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29225816/complement-dependent-cytotoxicity-and-luminex-technology-for-human-leucocyte-antigen-antibody-detection-in-kidney-transplant-candidates-exposed-to-different-sensitizing-events
#3
Nataša Katalinić, Alma Starčević, Martina Mavrinac, Sanja Balen
Background: The aim of this study was to determine the frequency of exposure to different sensitizing events (SEs) and to assess their effects on human leucocyte antigen (HLA) alloimmunization in transplant candidates using two different HLA antibody screening techniques: complement-dependent cytotoxicity (CDC) and Luminex. Methods: This retrospective study included HLA antibody screening results for 163 patients on the kidney transplant waiting list (WL) tested from March 2012 until the end of December 2015 at the Tissue Typing Laboratory, Rijeka, Croatia...
December 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/29217423/pre-transplant-at1r-antibodies-correlate-with-early-allograft-rejection
#4
Alison J Gareau, Chris Wiebe, Denise Pochinco, Ian W Gibson, Julie Ho, David N Rush, Peter W Nickerson
Studies investigating the potential pathogenic effects of non-HLA antibodies (Ab) have identified Ab against the angiotensin II type 1 receptor (AT1R-Ab) as a risk factor for rejection and kidney graft loss. This study sought to validate the risk of AT1R-Ab for acute rejection and to explore the role of other non-HLA Abs in this capacity. Pre- and post-transplant sera from a cohort of 101 patients (n=453 samples total) were tested for AT1R-Ab and other non-HLA Ab using a commercially available ELISA kit and the Luminex platform, respectively...
December 4, 2017: Transplant Immunology
https://www.readbyqxmd.com/read/29217402/inflammatory-profile-analysis-reveals-differences-in-cytokine-expression-between-smokers-moist-snuff-users-and-dual-users-compared-to-non-tobacco-consumers
#5
Judi Azevedo Sgambato, Bobbette A Jones, John W Caraway, G L Prasad
OBJECTIVE AND DESIGN: The aim of this study is to investigate the inflammatory alterations due to the use of smokeless tobacco and dual use of smokeless tobacco and cigarettes, relative to smoking. SUBJECTS: Plasma and saliva samples were collected from healthy smokers (SMK-100 subjects), moist snuff users (MSC-89 subjects), the dual users (DUSMK-49 subjects), and non-tobacco consumers (NTC-99 subjects) from two cross-sectional studies. METHODS: Luminex Human InflammationMAP® 1...
December 4, 2017: Cytokine
https://www.readbyqxmd.com/read/29210073/interleukin-17-regulates-matrix-metalloproteinase-activity-in-human-pulmonary-tuberculosis
#6
Shivani Singh, George Maniakis-Grivas, Utpal K Singh, Radha M Asher, Francesco Mauri, Paul T Elkington, Jon S Friedland
Tuberculosis (TB) is characterised by extensive pulmonary matrix breakdown. Interleukin-17 (IL-17) is key in host defence in TB but its role in TB-driven tissue damage is unknown. We investigated the hypothesis that respiratory stromal cell matrix metalloproteinase (MMP) production in TB is regulated by T helper-17 (TH -17) cytokines. Biopsies of patients with pulmonary TB were analysed by immunohistochemistry (IHC) and patient bronchoalveolar lavage fluid (BALF) MMP and cytokine concentrations measured by Luminex assays...
December 6, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/29203367/cross-talk-between-hydrogen-sulfide-and-carbon-monoxide-in-the-mechanism-of-experimental-gastric-ulcers-healing-regulation-of-gastric-blood-flow-and-accompanying-inflammation
#7
Marcin Magierowski, Katarzyna Magierowska, Magdalena Hubalewska-Mazgaj, Marcin Surmiak, Zbigniew Sliwowski, Mateusz Wierdak, Slawomir Kwiecien, Anna Chmura, Tomasz Brzozowski
Hydrogen sulfide (H2S) and carbon monoxide (CO) exert gastroprotection against acute gastric lesions. We determined the cross-talk between H2S and CO in gastric ulcer healing process and regulation of gastric blood flow (GBF) at ulcer margin. Male Wistar rats with acetic acid-induced gastric ulcers were treated i.g. throughout 9 days with vehicle (control), NaHS (0.1-10 mg/kg) +/- zinc protoporphyrin (ZnPP, 10 mg/kg), D,L-propargylglycine (PAG, 30 mg/kg), CO-releasing CORM-2 (2.5 mg/kg) +/- PAG. GBF was assessed by laser flowmetry, ulcer area was determined by planimetry/histology...
December 1, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29194572/the-igg-response-against-staphylococcus-aureus-is-associated-with-severe-atopic-dermatitis-in-children
#8
J E E Totté, L M Pardo, K B Fieten, J de Wit, D V de Boer, W J van Wamel, S G M A Pasmans
BACKGROUND: An altered immune response against Staphylococcus (S.) aureus might contribute to inflammation and barrier damage in atopic dermatitis (AD). OBJECTIVES: We profiled IgG antibodies against 55 S. aureus antigens in sera of children with mild to severe AD using a Luminex assay. Additionally, we evaluated the association between IgG levels and disease severity. METHODS: In this cross-sectional study, we included children with AD of two interventional study cohorts, namely SMA (n= 131) and the older DAVOS cohort (n= 76)...
November 30, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/29193362/donor-specific-anti-hla-antibodies-are-not-associated-with-non-anastomotic-biliary-strictures-but-both-are-independent-risk-factors-for-graft-loss-after-liver-transplantation
#9
Anne Claire den Dulk, Xiaolei Shi, Cornelia J Verhoeven, Jeroen Dubbeld, Frans H J Claas, Ron Wolterbeek, Simone H Brand-Schaaf, Hein W Verspaget, Arantza Fariña Sarasqueta, Luc J W van de Laan, Herold J Metselaar, Bart van Hoek, Jaap Kwekkeboom, Dave L Roelen
Donor-specific alloantibodies (DSA) have been associated with rejection and shorter graft survival after orthotopic liver transplantation (OLT). We examined the role of DSA in non-anastomotic biliary strictures (NAS) after OLT. Patients receiving first OLT who developed NAS (n=68) and a control group without NAS (n=83), with pre-OLT and 12 months post-OLT serum samples were included. DSA were specified using the Luminex single antigen test. Risk factors for NAS and graft survival were analysed. The presence of preformed DSA was not significantly different between patients with NAS and controls (p=0...
November 29, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/29188062/human-aqueous-humor-levels-of-transforming-growth-factor-%C3%AE-2-association-with-matrix-metalloproteinases-tissue-inhibitors-of-matrix-metalloproteinases
#10
Yan Jia, Yu Yue, Dan-Ning Hu, Ji-Li Chen, Ji-Bo Zhou
The present study aims to investigate the association of transforming growth factor-β2 (TGF-β2) and matrix metalloproteinases (MMPs), MMP-2 and MMP-3, and tissue inhibitors of matrix metalloproteinases (TIMPs), TIMP-1, TIMP-2 and TIMP-3 in the aqueous humor of patients with high myopia or cataracts. The levels of TGF-β2 and MMPs/TIMPs were measured with the Luminex xMAP Technology using commercially available Milliplex xMAP kits. The association between TGF-β2 and MMPs/TIMPs levels was analyzed using the Spearmans correlation test...
December 2017: Biomedical Reports
https://www.readbyqxmd.com/read/29184907/comparative-assessment-of-anti-hla-antibodies-using-two-commercially-available-luminex-based-assays
#11
Kevin J Clerkin, Sarah B See, Maryjane A Farr, Susan W Restaino, Geo Serban, Farhana Latif, Lingzhi Li, Paolo C Colombo, George Vlad, Bryan Ray, Elena R Vasilescu, Emmanuel Zorn
Background: Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2 main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms. Methods: Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients...
November 2017: Transplantation Direct
https://www.readbyqxmd.com/read/29183303/link-between-neurodegeneration-and-trabecular-meshwork-injury-in-glaucomatous-patients
#12
Yong Zhang, Qinmei Yang, Feng Guo, Xia Chen, Lin Xie
BACKGROUND: Glaucoma is classified as a neurodegenerative disease. However, the biomarkers of neurodegeneration in the aqueous humour of primary open angle glaucoma (POAG) eyes have not been quantitatively examined yet. In this study, levels of neurodegeneration-related cytokines in the aqueous humour of POAG eyes were measured and compared with those of non-glaucoma (senile cataract) control eyes. METHODS: This cross-sectional study included 24 patients (24 eyes) with POAG and 22 patients (22 eyes) with cataract...
November 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29182448/cerebrospinal-fluid-cytokine-expression-profile-in-multiple-sclerosis-and-chronic-inflammatory-demyelinating-polyneuropathy
#13
Serena Bonin, Nunzia Zanotta, Arianna Sartori, Alessio Bratina, Paolo Manganotti, Giusto Trevisan, Manola Comar
BACKGROUND: Cerebrospinal fluid (CSF) analysis in patients with particular neurologic disorders is a powerful tool to evaluate specific central nervous system inflammatory markers for diagnostic needs, because CSF represents the specific immune micro-environment to the central nervous system. METHODS: CSF samples from 49 patients with multiple sclerosis (MS), chronic inflammatory demyelinating polyneuropathy (CIDP), and non-inflammatory neurologic disorders (NIND) as controls were submitted to protein expression profiles of 47 inflammatory biomarkers by multiplex Luminex bead assay to investigate possible differences in the inflammatory process for MS and CIDP...
November 28, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/29181273/optimizing-peptide-epitope-based-autoantibody-detection-in-cancer-patients
#14
Maize Wang, Shirley H Lomeli, Wilbur A Franklin, Sarah Lee, Allan J Pantuck, Gang Zeng
Autoantibody (autoAb) response is an important arm of endogenously arising anti-tumor immune responses, and has received new attention as a cancer biomarker with the recent success of immune check-point inhibitor therapy. Our laboratory has been focusing on measuring autoAb against B-cell epitopes in order to bypass the necessity to purify a panel of recombinant proteins. In order to optimize peptide-based autoAb measurement and to increase sensitivities to cover more patients, we developed a new approach of using mixed peptides to conjugate on the same microsphere and compared its results with the use of a dominant peptide epitope using Luminex microbead-based multiplex assays...
2017: American Journal of Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29180164/th-17-cytokines-are-associated-with-severity-of-trypanosoma-cruzi-chronic-infection-in-pediatric-patients-from-endemic-areas-of-mexico
#15
Mariana De Alba-Alvarado, Paz María Salazar-Schettino, Luis Jiménez-Álvarez, Margarita Cabrera-Bravo, Cecilia García-Sancho, Edgar Zenteno, Clara Vazquez-Antona, Alfredo Cruz-Lagunas, Joaquín Zúñiga, Martha Irene Bucio-Torres
In Chagas disease the clinical, acute and chronic manifestations are the result of the interaction between the parasite and the host factors. The balance between inflammatory and anti-inflammatory immune responses is essential for the increase or resolution of the manifestations in individuals infected with T. cruzi. To identify if children with chronic Chagas disease and heart injury is related with non-regulated Th1, Th2 and Th17 responses. We included 31 children with T. cruzi confirmed chronic infection from endemic areas of Mexico...
November 24, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29175261/th17-cytokine-profiling-of-colorectal-cancer-patients-with-or-without-enterovirus-71-antigen-expression
#16
Yazhen Li, Qiancheng Qiu, Zhiqiang Fan, Ping He, Huanzhu Chen, Xiaoyang Jiao
OBJECT: Th17 cytokines have been identified in several types of human cancers. In this pilot study, the expression of Th17 cytokines profiling in enteroviruses 71 (EV71) associated colorectal cancer (CRC) were explored. METHODS: 66 patients with CRC were enrolled in this study; immune- histochemical analyses were performed on cancerous tissues and adjacent non- cancerous tissues of the patients. Serum Th17 cytokines of CRC patients and healthy controls were measured using a Luminex 200 analyzer...
November 23, 2017: Cytokine
https://www.readbyqxmd.com/read/29174861/evaluation-of-saliva-and-plasma-cytokine-biomarkers-in-patients-with-oral-squamous-cell-carcinoma
#17
L T Lee, Y K Wong, H Y Hsiao, Y W Wang, M Y Chan, K W Chang
The aim of this study was to investigate potential biomarkers in human saliva and plasma to aid in the early diagnosis of oral squamous cell carcinoma (OSCC). Saliva and plasma samples obtained from OSCC patients (n=41) and non-oral cancer patients (n=24) were analyzed by Luminex Bead-based Multiplex Assay. Data were analyzed using the non-parametric Mann-Whitney U-test, Kruskal-Wallis test, and receiver operating characteristics curve (ROC) to evaluate the predictive power of 14 biomarkers individually for OSCC diagnosis...
November 23, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29173617/identification-of-brucella-genus-and-eight-brucella-species-by-luminex-bead-based-suspension-array
#18
Tina S Lusk Pfefer, Ruth Timme, Julie A Kase
Globally, unpasteurized milk products are vehicles for the transmission of brucellosis, a zoonosis responsible for cases of foodborne illness in the United States and elsewhere. Existing PCR assays to detect Brucella species are restricted by the resolution of band sizes on a gel or the number of fluorescent channels in a single real-time system. The Luminex bead-based suspension array is performed in a 96-well plate allowing for high throughput screening of up to 100 targets in one sample with easily discernible results...
April 2018: Food Microbiology
https://www.readbyqxmd.com/read/29171655/oral-sampling-methods-are-associated-with-differences-in-immune-marker-concentrations
#19
Carole Fakhry, Fares Qeadan, Robert H Gilman, Pablo Yori, Margaret Kosek, Nicole Patterson, David W Eisele, Christine G Gourin, Chandala Chitguppi, Morgan Marks, Patti Gravitt
OBJECTIVE: To determine whether the concentration and distribution of immune markers in paired oral samples were similar. STUDY TYPE: Clinical research. STUDY DESIGN: Cross-sectional study. METHODS: Paired saliva and oral secretions (OS) samples were collected. The concentration of immune markers was estimated using Luminex multiplex assay (Thermo Fisher Scientific, Waltham, MA). For each sample, the concentration of respective immune markers was normalized to total protein present and log-transformed...
November 24, 2017: Laryngoscope
https://www.readbyqxmd.com/read/29168240/citrus-essential-oils-inhalation-by-mice-behavioral-testing-gcms-plasma-analysis-corticosterone-and-melatonin-levels-evaluation
#20
Adriana Nunes Wolffenbüttel, Amanda Zamboni, Gabriela Becker, Maíra Kerpel Dos Santos, Bruna Tassi Borille, Kristiane de Cássia Mariotti, Ana Claudia Fagundes, Janaína Lucas de Oliveira Salomón, Vanessa Rodrigues Coelho, Leire Virto Ruiz, Viviane de Moura Linck, Eliane Dallegrave, Pilar Cano, Ana Isabel Esquifino, Mirna Bainy Leal, Renata Pereira Limberger
The use of orange essential oils (EOs) as a complementary treatment is very common in Brazilian popular culture. The levels of melatonin (MEL) and corticosterone (CORT) hormones were investigated simultaneously, by the Luminex™ immunoassay system in mice plasma, after Citrus aurantium and Citrus sinensis EOs inhalation for 30 min. The plasma was analyzed by headspace through gas chromatography coupled to mass spectrometry for investigation of the EO components. Mice were submitted to behavioral testing to research anxiolytic-like, sedative, and antidepressant-like effects...
November 23, 2017: Phytotherapy Research: PTR
keyword
keyword
5937
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"