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https://www.readbyqxmd.com/read/29470350/analysis-of-luminex-based-algorithms-to-define-unacceptable-hla-antibodies-in-cdc-crossmatch-negative-kidney-transplant-recipients
#1
Daniel Zecher, Christian Bach, Adrian Preiss, Christoph Staudner, Kirsten Utpatel, Matthias Evert, Bettina Jung, Tobias Bergler, Carsten A Böger, Bernd M Spriewald, Bernhard Banas
BACKGROUND: HLA-specific antibodies detected by solid phase assays are increasingly used to define unacceptable HLA antigen mismatches (UAM) prior to renal transplantation. The accuracy of this approach is unclear. METHODS: Day of transplant sera from 211 CDC-crossmatch-negative patients were retrospectively analyzed for donor-specific anti-HLA antibodies (DSA) using Luminex technology. HLA were defined as UAM if DSA had mean fluorescence intensity above (I) 3000 (patients retransplanted and those with DSA against HLA class I and II) or 5000 (all other patients), (II) 5000 for HLA A, B and DR and 10,000 for HLA DQ or (III) 10,000 (all HLA)...
February 20, 2018: Transplantation
https://www.readbyqxmd.com/read/29468194/treatment-of-hiv-infected-individuals-with-the-histone-deacetylase-inhibitor-panobinostat-results-in-increased-numbers-of-regulatory-t-cells-and-limits-ex-vivo-lipopolysaccharide-induced-inflammatory-responses
#2
Christel Rothe Brinkmann, Jesper Falkesgaard Højen, Thomas Aagaard Rasmussen, Anne Sofie Kjær, Rikke Olesen, Paul W Denton, Lars Østergaard, Zhengyu Ouyang, Mathias Lichterfeld, Xu Yu, Ole Schmeltz Søgaard, Charles Dinarello, Martin Tolstrup
Histone deacetylase inhibitors (HDACi) modulate the transcriptional activity of all cells, including innate and adaptive immune cells. Therefore, we aimed to evaluate immunological effects of treatment with the HDACi panobinostat in HIV-infected patients during a clinical phase IIa latency reversal trial. Using flow cytometry, we investigated changes in T cell activation (CD69, CD38, HLA-DR) and the expression of CD39 and CTLA4 on regulatory T cells (Tregs). Whole-blood stimulations were performed and cytokine responses measured using Luminex...
January 2018: MSphere
https://www.readbyqxmd.com/read/29459262/soluble-endoglin-and-hypercholesterolemia-aggravate-endothelial-and-vessel-wall-dysfunction-in-mouse-aorta
#3
Barbora Vitverova, Katerina Blazickova, Iveta Najmanova, Matej Vicen, Radek Hyšpler, Eva Dolezelova, Ivana Nemeckova, Jurjen Duintjer Tebbens, Carmelo Bernabeu, Miguel Pericacho, Petr Nachtigal
BACKGROUND AND AIMS: Increased plasma levels of soluble endoglin (sEng) were detected in patients with endothelial dysfunction-related disorders and hypercholesterolemia. In this study, we hypothesized that high levels of sEng accompanied by mild hypercholesterolemia could aggravate endothelial and vessel wall dysfunction and affect endoglin/eNOS signaling in mouse aorta. METHODS: Three-month-old female transgenic mice on CBAxC57BL/6J background, with high levels of sEng (Sol-Eng+ high HFD), and their littermates with low levels of sEng (Sol-Eng+ low HFD), were fed a high fat diet for six months...
February 9, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29458388/a-novel-generation-1928zt2-car-t-cells-induce-remission-in-extramedullary-relapse-of-acute-lymphoblastic-leukemia
#4
Jianyu Weng, Peilong Lai, Le Qin, Yunxin Lai, Zhiwu Jiang, Chenwei Luo, Xin Huang, Suijing Wu, Dan Shao, Chengxin Deng, Lisi Huang, Zesheng Lu, Maohua Zhou, Lingji Zeng, Dongmei Chen, Yulian Wang, Xiaomei Chen, Suxia Geng, Weinkove Robert, Zhaoyang Tang, Chang He, Peng Li, Xin Du
BACKGROUND: Anti-CD19 chimeric antigen receptor (CAR) T cells have shown promise in the treatment of B cell acute lymphocytic leukemia (B-ALL). However, its efficacy in B-ALL patients with extramedullary involvement is limited due to poor responses and neurotoxicity. Here, we utilized a third generation of CAR T cell vector, which contains the Toll/interleukin-1 receptor (ITR) domain of Toll-like receptor 2 (TLR2), to generate 1928zT2 T cells targeting CD19, and evaluated the efficacy of 1928zT2 T cells in relapse or refractory B-ALL patients with extramedullary involvement...
February 20, 2018: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/29457879/the-good-the-bad-and-the-ugly-of-luminex-donor-specific-cross-match
#5
M Chowdhry, R N Makroo, Y Thakur, V Sharma, M Singh, M Kumar
The presence of donor specific antibodies directed against human leukocyte antigen significantly influences renal transplant due to antibody mediated rejection (AMR). We performed the screening of pre-renal transplant patients for preformed anti HLA antibodies using Antihuman globulin augmented-CDC crossmatch (AHG-CDCXm), Luminex donor specific crossmatch (LumXm) and HLA antibody screening. Seven hundred and fifty four patients were assessed for LumXm. HLA antibody screening was possible in 325 out of 754 cases...
February 19, 2018: HLA
https://www.readbyqxmd.com/read/29454023/interaction-between-hypothermia-and-delayed-mesenchymal-stem-cell-therapy-in-neonatal-hypoxic-ischemic-brain-injury
#6
Josephine Herz, Christian Köster, Barbara S Reinboth, Mark Dzietko, Wiebke Hansen, Hemmen Sabir, Cindy van Velthoven, Ivo Bendix, Ursula Felderhoff-Müser
Acute hypothermia treatment (HT) is the only clinically established intervention following neonatal hypoxic-ischemic brain injury. However, almost half of all cooled infants still die or suffer from long-lasting neurological impairments. Regenerative therapies, such as mesenchymal stem cells (MSC) appear promising as adjuvant therapy. In the present study, we hypothesized that HT combined with delayed MSC therapy results in augmented protection, improving long-term neurological outcome. Postnatal day 9 (P9) C57BL/6 mice were exposed to hypoxia-ischemia followed by 4 hours HT...
February 14, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29451216/does-combined-therapy-of-curcumin-and-epigallocatechin-gallate-have-a-synergistic-neuroprotective-effect-against-spinal-cord-injury
#7
Jiri Ruzicka, Lucia Machova Urdzikova, Barbora Svobodova, Anubhav G Amin, Kristyna Karova, Jana Dubisova, Kristyna Zaviskova, Sarka Kubinova, Meic Schmidt, Meena Jhanwar-Uniyal, Pavla Jendelova
Systematic inflammatory response after spinal cord injury (SCI) is one of the factors leading to lesion development and a profound degree of functional loss. Anti-inflammatory compounds, such as curcumin and epigallocatechin gallate (EGCG) are known for their neuroprotective effects. In this study, we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression. Immediately after SCI, rats received curcumin, EGCG, curcumin + EGCG or saline [daily intraperitoneal doses (curcumin, 6 mg/kg; EGCG 17 mg/kg)] and weekly intramuscular doses (curcumin, 60 mg/kg; EGCG 17 mg/kg)] for 28 days...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29449599/red-blood-cells-are-dynamic-reservoirs-of-cytokines
#8
Elisabeth Karsten, Edmond Breen, Benjamin R Herbert
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex ® technology...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29445381/proteins-of-tnf-%C3%AE-and-il6-pathways-are-elevated-in-serum-of-type-1-diabetes-patients-with-microalbuminuria
#9
Sharad Purohit, Ashok Sharma, Wenbo Zhi, Shan Bai, Diane Hopkins, Leigh Steed, Bruce Bode, Stephen W Anderson, John Chip Reed, R Dennis Steed, Jin-Xiong She
Soluble cytokine receptors may play an important role in development of microalbuminuria (MA) in type-1 diabetes (T1D). In this study, we measured 12 soluble receptors and ligands from TNF-α/IL6/IL2 pathways in T1D patients with MA ( n = 89) and T1D patients without MA ( n = 483) participating in the PAGODA study. Twelve proteins in the sera from T1D patients with and without MA were measured using multiplex Luminex assays. Ten serum proteins (sTNFR1, sTNFR2, sIL2Rα, MMP2, sgp130, sVCAM1, sIL6R, SAA, CRP, and sICAM1) were significantly elevated in T1D patients with MA...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29439934/suppressed-networks-of-inflammatory-mediators-characterize-chronic-venous-insufficiency
#10
Ulka Sachdev, Lena Vodovotz, Julie Bitner, Derek Barclay, Ruben Zamora, Jinling Yin, Richard L Simmons, Yoram Vodovotz
OBJECTIVE: Chronic venous insufficiency (CVI) affects 25 million adults in the United States. Little emphasis has been placed on inflammatory changes associated with CVI. We hypothesize that in patients with early to mid-stage benign varicose vein disease, differences in circulating inflammatory mediators will be manifested in blood draining the involved area vs circulating blood in control subjects. METHODS: Patients undergoing either endovenous ablation or sclerotherapy for Clinical, Etiology, Anatomy, and Pathophysiology clinical class 3 to 5 disease underwent phlebotomy from regional veins at the time of the procedure...
February 10, 2018: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/29428309/mycoplasma-genitalium-can-modulate-the-local-immune-response-in-patients-with-endometriosis
#11
Guilherme B Campos, Lucas M Marques, Izadora S Rezende, Maysa S Barbosa, Mauricio S Abrão, Jorge Timenetsky
OBJECTIVE: To detect Mollicutes in women with endometriosis and healthy peritoneal tissues and evaluate the participation of these bacteria in the immune response during endometriosis. DESIGN: Cross-sectional study. SETTING: University hospitals. PATIENT (S): Women with endometriosis (n = 73) and without endometriosis (n = 31). INTERVENTION(S): Endocervical swabs, peritoneal fluid, and biopsied lesions of endometriosis of women with endometriosis (study group) and healthy peritoneal tissues (control group) were collected during surgery...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29428178/evaluation-on-the-persistence-of-anti-hpv-immune-responses-to-the-quadrivalent-hpv-vaccine-in-chinese-females-and-males-up-to-3-5%C3%A2-years-of-follow-up
#12
Teng Huang, Youping Liu, Yanping Li, Yuqin Liao, Qiong Shou, Minghuan Zheng, Xueyan Liao, Rongcheng Li
BACKGROUND: This was an extension study of a randomized, double-blind, placebo-controlled immunogenicity and safety study of the quadrivalent human papillomavirus (qHPV) (HPV 6, 11, 16, and 18) vaccine conducted in Chinese female subjects aged 9-45 years and male subjects aged 9-15 years. To investigate the persistence of anti-HPV 6, -11, -16, and -18 responses among Chinese subjects, subjects enrolled in the base study were followed up at around month 42 (approximately 3.5 years after vaccination)...
February 7, 2018: Vaccine
https://www.readbyqxmd.com/read/29427404/interleukin-8-mediates-neutrophil-endothelial-interactions-in-pig-to-human-xenogeneic-models
#13
Beth M French, Selin Sendil, Krishna Mohan Sepuru, Jolene Ranek, Lars Burdorf, Donald Harris, Emily Redding, Xiangfei Cheng, Christopher T Laird, Yuming Zhao, Benjamin Cerel, Krishna Rajarathnam, Richard N Pierson, Agnes M Azimzadeh
BACKGROUND: Human neutrophils are sequestered by pig lung xenografts within minutes during ex vivo perfusion. This phenomenon is not prevented by pig genetic modifications that remove xeno-antigens or added human regulatory molecules intended to down-regulate activation of complement and coagulation pathways. This study investigated whether recipient and donor interleukin-8 (IL-8), a chemokine known to attract and activate neutrophils during inflammation, is elaborated in the context of xenogeneic injury, and whether human or pig IL-8 promote the adhesion of human neutrophils in in vitro xenograft models...
February 9, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29426834/value-of-circulating-cytokine-profiling-during-submaximal-exercise-testing-in-myalgic-encephalomyelitis-chronic-fatigue-syndrome
#14
Kegan J Moneghetti, Mehdi Skhiri, Kévin Contrepois, Yukari Kobayashi, Holden Maecker, Mark Davis, Michael Snyder, Francois Haddad, Jose G Montoya
Myalgic Encephalomyelitis or Chronic Fatigue Syndrome (ME/CFS) is a heterogeneous syndrome in which patients often experience severe fatigue and malaise following exertion. Immune and cardiovascular dysfunction have been postulated to play a role in the pathophysiology. We therefore, examined whether cytokine profiling or cardiovascular testing following exercise would differentiate patients with ME/CFS. Twenty-four ME/CFS patients were matched to 24 sedentary controls and underwent cardiovascular and circulating immune profiling...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29422548/africa-wide-evaluation-of-host-biomarkers-in-quantiferon-supernatants-for-the-diagnosis-of-pulmonary-tuberculosis
#15
Novel N Chegou, Jayne S Sutherland, Anna-Ritah Namuganga, Paul Lam Corstjens, Annemieke Geluk, Gebremedhin Gebremichael, Joseph Mendy, Stephanus Malherbe, Kim Stanley, Gian D van der Spuy, Magdalena Kriel, Andre G Loxton, Belinda Kriel, Felanji Simukonda, Yonas Bekele, Jacob A Sheehama, Josefina Nelongo, Marieta van der Vyver, Atsbeha Gebrexabher, Habteyes Hailu, Maria M Esterhuyse, Ida Rosenkrands, Claus Aagard, Martin Kidd, Desta Kassa, Adane Mihret, Rawleigh Howe, Jacqueline M Cliff, Amelia C Crampin, Harriet Mayanja-Kizza, Stefan H E Kaufmann, Hazel M Dockrell, Tom H M Ottenhoff, Gerhard Walzl
We investigated host-derived biomarkers that were previously identified in QuantiFERON supernatants, in a large pan-African study. We recruited individuals presenting with symptoms of pulmonary TB at seven peripheral healthcare facilities in six African countries, prior to assessment for TB disease. We then evaluated the concentrations of 12 biomarkers in stored QuantiFERON supernatants using the Luminex platform. Based on laboratory, clinical and radiological findings and a pre-established algorithm, participants were classified as TB disease or other respiratory diseases(ORD)...
February 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29420060/platelets-regulate-pulmonary-inflammation-and-tissue-destruction-in-tuberculosis
#16
Katharine A Fox, Daniela E Kirwan, Ashley M Whittington, Nitya Krishnan, Brian D Robertson, Robert H Gilman, José W López, Shivani Singh, Joanna C Porter, Jon S Friedland
RATIONALE: Platelets may interact with the immune system in tuberculosis (TB) to regulate human inflammatory responses that lead to morbidity and spread of infection. OBJECTIVES: To identify a functional role of platelets in the innate inflammatory and matrix degrading response in TB. METHODS: Markers of platelet activation were examined in plasma from 50 TB patients pre-treatment, and 50 controls. 25 patients were followed longitudinally...
February 8, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29417469/association-between-systemic-leptin-and-neurotensin-concentration-in-adult-individuals-with-and-without-type-2-diabetes-mellitus
#17
I Barchetta, G Ciccarelli, F A Cimini, V Ceccarelli, M Orho-Melander, O Melander, M G Cavallo
PURPOSE: Leptin is an adipokine which regulates appetite and energy balance through a mechanism partially mediated by neurotensin (NT) in central nervous system. Besides acting as a neurotransmitter, NT is expressed in human intestine where it promotes fat absorption and its circulating levels associate with obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease. Whether a relation exists between circulating leptin and NT levels has not been investigated yet. The aim of this study was to test the hypothesis of an association between plasma leptin and NT concentration in adults with or without T2DM...
February 7, 2018: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29413515/baseline-serum-cxcl10-and-il-12-levels-may-predict-severe-asthmatics-responsiveness-to-omalizumab
#18
Maho Suzukawa, Hisako Matsumoto, Nobuharu Ohshima, Hiroyuki Tashimo, Isao Asari, Tomoko Tajiri, Akio Niimi, Hiroyuki Nagase, Hirotoshi Matsui, Nobuyuki Kobayashi, Shunsuke Shoji, Ken Ohta
BACKGROUND: Omalizumab, a humanized anti-IgE monoclonal antibody, is the first molecularly targeted drug for severe asthmatics. However, responses to omalizumab vary widely among patients. OBJECTIVES: This study aimed to assess the potential of baseline serum cytokine levels as predictors of responsiveness to omalizumab. METHODS: Thirty-one patients with severe, persistent asthma were enrolled in this study and administered omalizumab for at least 1 year...
January 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29413111/subclinical-mastitis-scm-and-proinflammatory-cytokines-are-associated-with-mineral-and-trace-element-concentrations-in-human-breast-milk
#19
Chen Li, Noel W Solomons, Marilyn E Scott, Kristine G Koski
The possibility that either subclinical mastitis (SCM), an inflammatory condition of the breast, or elevations in breast milk proinflammatory cytokines alter breast milk mineral and trace element composition in humans has not been investigated. In this cross-sectional study, breast milk samples (n=108) were collected from Guatemalan Mam-Mayan mothers at one of three stages of lactation (transitional, early and established), and categorized as SCM (Na:K >0.6) or non-SCM (Na:K ≤0.6). Milk concentrations of 12 minerals (calcium, copper, iron, magnesium, manganese, phosphorus, potassium, rubidium, selenium, sodium, strontium, and zinc) and 4 proinflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α) were measured by inductively coupled plasma mass spectrometry (ICP-MS), Lachat analyzer or Luminex multiplex bead cytokine assay...
March 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29401272/identifying-immune-correlates-of-protection-against-plasmodium-falciparum-through-a-novel-approach-to-account-for-heterogeneity-in-malaria-exposure
#20
Aida Valmaseda, Eusebio Macete, Augusto Nhabomba, Caterina Guinovart, Pedro Aide, Azucena Bardají, Quique Bassat, Tacilta Nhampossa, Sonia Maculuve, Aina Casellas, Llorenç Quintó, Sergi Sanz, Alfons Jiménez, Gaoqian Feng, Christine Langer, Linda Reiling, K Sony Reddy, Alok Pandey, Chetan E Chitnis, Virander S Chauhan, Ruth Aguilar, John J Aponte, Carlota Dobaño, James G Beeson, Deepak Gaur, Clara Menéndez, Pedro L Alonso, Alfredo Mayor
Background: A main criterion to identify malaria vaccine candidates is the proof that acquired immunity against them is associated with protection from disease. The age of the studied individuals, heterogeneous malaria exposure, and assumption of the maintenance of a baseline immune response can confound these associations. Methods: Immunoglobulin G/immunoglobulin M (IgG/ IgM) levels were measured by Luminex® in Mozambican children monitored for clinical malaria from birth until 3 years of age, together with functional antibodies...
February 1, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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