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https://www.readbyqxmd.com/read/28077518/th17-cytokine-responses-in-lyme-disease-correlate-with-borreliaburgdorferi-antibodies-during-early-infection-in-patients-with-erythema-migrans-and-with-autoantibodies-late-in-the-illness-in-patients-with-antibiotic-refractory-lyme-arthritis
#1
Klemen Strle, Katherine B Sulka, Annalisa Pianta, Jameson T Crowley, Sheila L Arvikar, Anthony Anselmo, Ruslan Sadreyev, Allen C Steere
BACKGROUND: Control of Lyme disease is attributed predominantly to innate and adaptive TH1 immune responses, whereas the role of TH17 responses is less clear. Here we characterized these inflammatory responses in patients with erythema migrans (EM) or Lyme arthritis (LA) to elucidate their role early and late in the infection. METHODS: Levels of 21 cytokines and chemokines, representative of innate, TH1, and TH17 immune responses, were assessed by Luminex in acute and convalescent sera from 91 EM patients, in serum and synovial fluid from 141 LA patients, and in serum from 57 healthy subjects...
January 11, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28076513/serum-concentration-of-vascular-endothelial-growth-factor-and-depth-of-trophoblastic-invasion-in-ampullary-ectopic-pregnancy
#2
Fábio Roberto Cabar, Décio Roberto Kamio Teshima, Pedro Paulo Pereira, Leila Antonangelo, Regina Schultz, Rossana Pulcineli Francisco
OBJECTIVE: To evaluate the association between the depth of trophoblastic infiltration and serum vascular endothelial growth factorconcentration in patients with an ampullary pregnancy. METHODS: This prospective cross-sectionalstudy involved 34 patients with an ampullary ectopic pregnancy who underwent salpingectomy between 2012 and 2013. Maternal serum vascular endothelial growth factor concentrations were measured using Luminex technology. Trophoblastic invasion was classified histologically as follows: stage I, limited to the tubal mucosa; stage II, reaching the muscle layer; and stage III,involving the full thickness...
December 1, 2016: Clinics
https://www.readbyqxmd.com/read/28074537/neuroblastoma-causes-alterations-of-the-intestinal-microbiome-gut-hormones-inflammatory-cytokines-and-bile-acid-composition
#3
Christoph Castellani, Georg Singer, Margarita Kaiser, Thomas Kaiser, Jianfeng Huang, Daniela Sperl, Karl Kashofer, Guenter Fauler, Barbara Guertl-Lackner, Gerald Höfler, Holger Till
OBJECTIVE: To assess the effect of neuroblastoma (NB) on the intestinal microbiome, metabolism, and inflammatory parameters in a murine model. MATERIALS AND METHODS: Athymic Hsd:Fox1nu mice received subperitoneal implantation of human NB cells (MHH-NB11) (tumor group, TG) or culture medium (sham group). Following 10 weeks of tumor growth, all animals were sacrificed to collect total white adipose tissue (WAT). Luminex assays were performed for gut hormone and inflammation marker analysis...
January 11, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28073175/post-reperfusion-microcirculatory-derangements-after-liver-transplantation-relationship-to-hemodynamics-serum-mediators-and-outcome
#4
Carlo Pulitano, David Joseph, Charbel Sandroussi, Deborah Verran, Phong Ho, Ashe Debiasio, Adriano Luongo, Geoffrey W McCaughan, Nicholas Shackel, Michael Crawford
INTRODUCTION: Despite the growing data supporting the role of microcirculation in regulating liver function, little of this knowledge has been translated into clinical practice. The aim of this study is to quantify hepatic microcirculation in-vivo using sidestream dark-field (SDF) imaging and correlate these finding with hepatic blood flow, hemodynamic parameters, and soluble mediators. METHODS: Post-reperfusion hepatic microcirculation was assessed using SDF imaging...
January 10, 2017: Liver Transplantation
https://www.readbyqxmd.com/read/28073031/plasma-concentrations-of-free-amyloid-%C3%AE-cannot-predict-the-development-of-alzheimer-s-disease
#5
Hugo Lövheim, Fredrik Elgh, Anders Johansson, Henrik Zetterberg, Kaj Blennow, Göran Hallmans, Sture Eriksson
INTRODUCTION: Biomarkers that identify individuals at risk of Alzheimer's disease (AD) development would be highly valuable. Plasma concentration of amyloid β (Aβ)-central in the pathogenesis of AD-is a logical candidate, but studies to date have produced conflicting results on its utility. METHODS: Plasma samples from 339 preclinical AD cases (76.4% women, mean age 61.3 years) and 339 age- and sex-matched dementia-free controls, taken an average of 9.4 years before AD diagnosis, were analyzed using Luminex xMAP technology and INNOBIA plasma Aβ form assays to determine concentrations of free plasma Aβ40 and Aβ42...
January 7, 2017: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
https://www.readbyqxmd.com/read/28072560/a-prostacyclin-analog-cicaprost-exhibits-potent-anti-inflammatory-activity-in-human-primary-immune-cells-and-a-uveitis-model
#6
Jenny W Wang, Chau Vu, Neil J Poloso
PURPOSE: To investigate the therapeutic potential of a prostacyclin (IP) receptor agonist for ocular inflammation and the effect on immune cells. METHODS: The anti-inflammatory activities of cicaprost were determined in primary human monocyte-derived macrophages and human monocyte-derived dendritic cells (MoDC), as well as a lipopolysaccharides (LPS)-induced rat uveitis model. Multiple cytokine release was measured by utilizing Luminex Technology. Prostacyclin (IP) Receptor expression was detected by reverse transcription-polymerase chain receptor...
January 10, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28070761/effect-of-long-term-simulated-microgravity-on-immune-system-and-lung-tissues-in-rhesus-macaque
#7
Yang Chen, Chongyu Xu, Ping Wang, Yiling Cai, Huasong Ma
We evaluated different lymphocyte populations and levels of plasma cytokines in peripheral blood as well as inflammatory infiltration and expressions of cytokines in lung tissues derived from macaque under long-term stimulated microgravity through being suspended in an antiorthostatic position so as to identify relevant immune parameters and to understand potential mechanisms of lung injury. Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n = 5), groups 2 (head-down tilting for 6 weeks, n = 5), and groups 3 (head-down tilting for 6 weeks and recovery for 4 weeks, n = 5)...
January 9, 2017: Inflammation
https://www.readbyqxmd.com/read/28068936/retrospective-study-of-ras-pik3ca-braf-tumor-mutations-as-predictors-of-response-to-first-line-chemotherapy-with-bevacizumab-in-metastatic-colorectal-cancer-patients
#8
Izuma Nakayama, Eiji Shinozaki, Tomohiro Matsushima, Takeru Wakatsuki, Mariko Ogura, Takashi Ichimura, Masato Ozaka, Daisuke Takahari, Mitsukuni Suenaga, Keisho Chin, Nobuyuki Mizunuma, Kensei Yamaguchi
BACKGROUND: After analysis of minor RAS mutations (KRAS exon 3, 4/NRAS) in the FIRE-3 and PRIME studies, an expanded range of RAS mutations were established as a negative predictive marker for the efficacy of anti-EGFR antibody treatment. BRAF and PIK3CA mutations may be candidate biomarkers for anti-EGFR targeted therapies. However, it remains unknown whether RAS/PIK3CA/BRAF tumor mutations can predict the efficacy of bevacizumab in metastatic colorectal cancer. We assessed whether selection according to RAS/PIK3CA/BRAF mutational status could be beneficial for patients treated with bevacizumab as first-line treatment for metastatic colorectal cancer...
January 9, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28068934/concordance-between-pik3ca-mutations-in-endoscopic-biopsy-and-surgically-resected-specimens-of-esophageal-squamous-cell-carcinoma
#9
Ken Hatogai, Satoshi Fujii, Takashi Kojima, Hiroyuki Daiko, Toshihiko Doi, Atsushi Ohtsu, Atsushi Ochiai, Yuichi Takiguchi, Takayuki Yoshino
BACKGROUND: PIK3CA mutations are expected to be potential therapeutic targets for esophageal squamous cell carcinoma (ESCC). We aimed to clarify the concordance between PIK3CA mutations detected in endoscopic biopsy specimens and corresponding surgically resected specimens. METHODS: We examined five hotspot mutations in the PIK3CA gene (E542K, E545K, E546K, H1047R, and H1047L) in formalin-fixed and paraffin-embedded tissue sections of paired endoscopic biopsy and surgically resected specimens from 181 patients undergoing curative resection for ESCC between 2000 and 2011 using a Luminex technology-based multiplex gene mutation detection kit...
January 9, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28068297/fatty-acid-derivative-chemokine-and-cytokine-profiles-in-exhaled-breath-condensates-can-differentiate-adult-and-children-paucibacillary-tuberculosis-patients
#10
Sergio Fabián Mosquera-Restrepo, Ana Cecilia Caro, Luis F García, Carlos Alberto Peláez-Jaramillo, Mauricio Rojas
The anti-mycobacterial immune response in adults and children with tuberculosis (TB), as well as the response in bacteriologically positive and negative patients, is different. However, knowledge of the immunological events occurring in the lungs in these clinical situations remains scarce. Exhaled breath condensate (EBC) samples may be useful for studying the inflammatory environment of the lower airways in TB patients. The fatty acid, cytokine, and chemokine profiles in EBC from healthy adults; smear-positive and smear-negative adult patients; and healthy, asthmatic, and TB children were determined using gas chromatography and LUMINEX, respectively...
January 9, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28067251/analgesic-and-anti-inflammatory-effects-of-the-novel-semicarbazide-sensitive-amine-oxidase-inhibitor-szv-1287-in-chronic-arthritis-models-of-the-mouse
#11
Ádám Horváth, Awt Menghis, Bálint Botz, Éva Borbély, Ágnes Kemény, Valéria Tékus, Janka Zsófia Csepregi, Attila Mócsai, Tamás Juhász, Róza Zákány, Dóra Bogdán, Péter Mátyus, Julie Keeble, Erika Pintér, Zsuzsanna Helyes
Semicarbazide-sensitive amine oxidase (SSAO) catalyses oxidative deamination of primary amines. Since there is no data about its function in pain and arthritis mechanisms, we investigated the effects of our novel SSAO inhibitor SzV-1287 in chronic mouse models of joint inflammation. Effects of SzV-1287 (20 mg/kg i.p./day) were investigated in the K/BxN serum-transfer and complete Freund's adjuvant (CFA)-evoked active immunization models compared to the reference SSAO inhibitor LJP-1207. Mechanonociception was assessed by aesthesiometry, oedema by plethysmometry, clinical severity by scoring, joint function by grid test, myeloperoxidase activity by luminescence, vascular leakage by fluorescence in vivo imaging, histopathological changes by semiquantitative evaluation, and cytokines by Luminex assay...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28063043/subcutaneous-and-sublingual-immunotherapy-in-a-mouse-model-of-allergic-asthma
#12
Laura Hesse, Martijn C Nawijn
Allergic asthma, caused by inhaled allergens such as house dust mite or grass pollen, is characterized by reversible airway obstruction, associated with an eosinophilic inflammation of the airways, as well as airway hyper responsiveness and remodeling. The inhaled allergens trigger a type-2 inflammatory response with involvement of innate lymphoid cells (ILC2) and Th2 cells, resulting in high production of immunoglobulin E (IgE) antibodies. Consequently, renewed allergen exposure results in a classic allergic response with a distinct early and late phase, both resulting in bronchoconstriction and shortness of breath...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28060224/granulocytic-myeloid-derived-suppressor-cells-increased-in-early-phases-of-primary-hiv-infection-depending-on-trail-plasma-level
#13
N Tumino, M T Bilotta, C Pinnetti, A Ammassari, A Antinori, F Turchi, C Agrati, R Casetti, V Bordoni, E Cimini, I Abbate, M R Capobianchi, F Martini, A Sacchi
BACKGROUND: It has been demonstrated that Myeloid Derived Suppressor Cells (MDSC) are expanded in HIV-1 infected individuals and correlated with disease progression. The phase of HIV infection during which MDSC expansion occurs, and the mechanisms that regulate this expansion remain to be established. In this study we evaluated the frequency of MDSC in patients during primary HIV infection, and factors involved in MDSC control. METHODS: Patients with primary (PHI) and chronic (CHI) HIV infection were enrolled...
January 2, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28045875/conformational-variants-of-hla-i-antigens-on-luminex-single-antigen-beads-for-monitoring-antibodies
#14
Mepur H Ravindranath, Vadim Jucaud
No abstract text is available yet for this article.
December 31, 2016: Transplantation
https://www.readbyqxmd.com/read/28041935/role-of-interleukin-23-in-monocyte-derived-dendritic-cells-of-hbv-related-acute-on-chronic-liver-failure-and-its-correlation-with-the-severity-of-liver-damage
#15
Suxia Bao, Jianming Zheng, Ning Li, Chong Huang, Mingquan Chen, Qi Cheng, Qian Li, Qing Lu, Mengqi Zhu, Qingxia Ling, Kangkang Yu, Shengshen Chen, Guangfeng Shi
BACKGROUND: The role of interleukin-23 (IL-23) in monocyte-derived dendritic cells (MoDCs) from hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) patients remains unclear. The aim of this study was to observe the correlation between the activation of the IL-23 signaling pathway and the prognosis of HBV-ACLF patients. MATERIALS AND METHODS: The baseline levels of serum IL-6, IL-12, IL-17, IL-23, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) from immune tolerant (IT), chronic hepatitis B (CHB), HBV-ACLF patients and healthy individuals who served as healthy controls (HCs) were analyzed using the Luminex system, whereas serum IL-23 from HBV-ACLF patients was measured by ELISA before and after treatment...
December 29, 2016: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/28032635/dexamethasone-promotes-granulocyte-mobilization-by-prolonging-the-half-life-of-granulocyte-colony-stimulating-factor-in-healthy-donors-for-granulocyte-transfusions
#16
Ida H Hiemstra, John L van Hamme, Machiel H Janssen, Timo K van den Berg, Taco W Kuijpers
BACKGROUND: Granulocyte transfusion (GTX) is a potential approach to correcting neutropenia and relieving the increased risk of infection in patients who are refractory to antibiotics. To mobilize enough granulocytes for transfusion, healthy donors are premedicated with granulocyte-colony-stimulating factor (G-CSF) and dexamethasone. Granulocytes have a short circulatory half-life. Consequently, patients need to receive GTX every other day to keep circulating granulocyte counts at an acceptable level...
December 28, 2016: Transfusion
https://www.readbyqxmd.com/read/28025511/immunological-roles-of-elevated-plasma-levels-of-matricellular-proteins-in-japanese-patients-with-pulmonary-tuberculosis
#17
Beata Shiratori, Jingge Zhao, Masao Okumura, Haorile Chagan-Yasutan, Hideki Yanai, Kazue Mizuno, Takashi Yoshiyama, Tadashi Idei, Yugo Ashino, Chie Nakajima, Yasuhiko Suzuki, Toshio Hattori
Elevated matricellular proteins (MCPs), including osteopontin (OPN) and galectin-9 (Gal-9), were observed in the plasma of patients with Manila-type tuberculosis (TB) previously. Here, we quantified plasma OPN, Gal-9, and soluble CD44 (sCD44) by enzyme-linked immunosorbent assay (ELISA), and another 29 cytokines by Luminex assay in 36 patients with pulmonary TB, six subjects with latent tuberculosis (LTBI), and 19 healthy controls (HCs) from Japan for a better understanding of the roles of MCPs in TB. All TB subjects showed positive results of enzyme-linked immunospot assays (ELISPOTs)...
December 22, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28019029/hla-drb3-01-01-is-a-predictor-of-immunization-against-human-platelet-antigen-1a-but-not-of-the-severity-of-fetal-and-neonatal-alloimmune-thrombocytopenia
#18
Sandra Wienzek-Lischka, Inke R König, Eva-Maria Papenkort, Holger Hackstein, Sentot Santoso, Ulrich J Sachs, Gregor Bein
BACKGROUND: Most cases of fetal and neonatal alloimmune thrombocytopenia (FNAIT) are caused by maternal alloantibodies against human platelet antigen-1a (HPA-1a). Alloimmunization mainly occurs in HPA-1a-negative mothers who are carriers of the HLA-DRB3*01:01 allele. Recently, it has been reported that the combined presence of HLA-DRB3*01:01 and HLA-DRB4*01:01P was associated with severity of FNAIT. We tested this hypothesis by analyzing a large cohort of cases and controls. STUDY DESIGN AND METHODS: In total, 101 mothers with a history of FNAIT caused by anti-HPA-1a were investigated...
December 26, 2016: Transfusion
https://www.readbyqxmd.com/read/28018342/detection-of-hla-antibodies-in-organ-transplant-recipients-triumphs-and-challenges-of-the-solid-phase-bead-assay
#19
REVIEW
Brian D Tait
This review outlines the development of human leukocyte antigen (HLA) antibody detection assays and their use in organ transplantation in both antibody screening and crossmatching. The development of sensitive solid phase assays such as the enzyme-linked immunosorbent assay technique, and in particular the bead-based technology has revolutionized this field over the last 10-15 years. This revolution however has created a new paradigm in clinical decision making with respect to the detection of low level pretransplant HLA sensitization and its clinical relevance...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/28012451/activation-of-the-cholinergic-anti-inflammatory-pathway-by-nicotine-ameliorates-lipopolysaccharide-induced-preeclampsia-like-symptoms-in-pregnant-rats
#20
Yuanyuan Liu, Jinying Yang, Junjie Bao, Xiaolan Li, Aihua Ye, Guozheng Zhang, Huishu Liu
INTRODUCTION: Preeclampsia (PE) exerts a more intense systemic inflammatory response than normal pregnancy. Recently, the role of the cholinergic anti-inflammatory pathway (CAP) in regulating inflammation has been extensively studied. The aim of this study was to investigate the effect of nicotine, a selective cholinergic agonist, on lipopolysaccharide (LPS)-induced preeclampsia-like symptoms in pregnant rats and to determine the molecular mechanism underlying it. METHODS: Rats were administered LPS (1...
January 2017: Placenta
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