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https://www.readbyqxmd.com/read/29914680/survey-of-cytokines-in-normal-canine-tears-by-multiplex-analysis-a-pilot-study
#1
Paoul S Martinez, Eric S Storey, Cherie M Pucheu-Haston
OBJECTIVES: The purpose of this pilot study was to use a multiplexed assay to measure cytokines in normal stimulated canine tears. METHODS: 25 healthy dogs were included in the study. Stimulated tears were collected in capillary tubes from the right (OD) and left (OS) eyes and stored at -80 °C until batch sample analysis was performed. The samples were analyzed utilizing Luminex® canine-validated multiplex beads on a Bio-Rad multiplex analyzer for IL-2, IL-6, IL-7, IL-8, IL-10, TNF-α, and IFN-γ...
July 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29908520/detection-of-norovirus-by-bd-max%C3%A2-xpert-%C3%A2-norovirus-and-xtag-%C3%A2-gastrointestinal-pathogen-panel-in-stool-and-vomit-samples
#2
Martin P McHugh, Daniel Guerendiain, Alison Hardie, Juliet Kenicer, Laura MacKenzie, Kate E Templeton
BACKGROUND: Norovirus is a leading cause of infectious gastroenteritis, characterized by outbreaks of diarrhoea and vomiting in closed settings. Nucleic acid amplification tests allow rapid and sensitive laboratory diagnosis of norovirus, with a number of commercial platforms now available. OBJECTIVES: Evaluate the performance of the Becton Dickinson BD-MAX™System, Cepheid Xpert® Norovirus Assay, and Luminex xTAG® Gastrointestinal Pathogen Panel (GPP) for norovirus detection in stool...
June 8, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29904439/multiplexed-microsphere-suspension-array-assay-for-urine-mitochondrial-dna-typing-by-c-stretch-length-in-hypervariable-regions
#3
Kimiko Aoki, Hiroyuki Tanaka, Takashi Kawahara
Background: The standard method for personal identification and verification of urine samples in doping control is short tandem repeat (STR) analysis using nuclear DNA (nDNA). The DNA concentration of urine is very low and decreases under most conditions used for sample storage; therefore, the amount of DNA from cryopreserved urine samples may be insufficient for STR analysis. We aimed to establish a multiplexed assay for urine mitochondrial DNA typing containing only trace amounts of DNA, particularly for Japanese populations...
July 2018: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29903545/evaluation-of-t-cells-in-blood-after-a-short-gluten-challenge-for-coeliac-disease-diagnosis
#4
Natalia López-Palacios, Virginia Pascual, Mercedes Castaño, Andrés Bodas, Marta Fernández-Prieto, Laura Espino-Paisán, Eva Martínez-Ojinaga, Isabel Salazar, Raquel Martínez-Curiel, Enrique Rey, Lourdes Estrada, Magdalena Molero-Abraham, Pedro A Reche, Romina Dieli-Crimi, Concepción Núñez
BACKGROUND AND AIM: To diagnose coeliac disease (CD) in individuals on a gluten free diet (GFD), we aimed to assess the utility of detecting activated γδ and CD8 T cells expressing gut-homing receptors after a short gluten challenge. METHODS: We studied 15 CD patients and 35 non-CD controls, all exposed to three days of gluten when following a GFD. Peripheral blood was collected before and six days after starting gluten consumption, and the expression of CD103, β7 and CD38 in γδ and CD8 T cells was assessed by flow cytometry...
April 27, 2018: Digestive and Liver Disease
https://www.readbyqxmd.com/read/29899000/clinical-evaluation-of-the-icubate-ic-gpc-assay%C3%A2-for-detection-of-gram-positive-bacteria-and-resistance-markers-from-positive-blood-cultures
#5
Paul A Granato, Melissa M Unz, Raymond H Widen, Suzane Silbert, Stephen Young, Kathryn L Heflin, Matt S Conover, Blake W Buchan, Nathan A Ledeboer
The iC-GPC Assay™ (iCubate®, Huntsville, AL) is a qualitative, multiplex test for the detection of five of the most common gram-positive bacteria ( S. aureus, S. epidermidis, S. pneumoniae, E. faecalis, and E. faecium ) responsible for bacterial bloodstream infections directly from positive blood cultures. The assay also detects the presence of the mec A, van A, and van B resistance determinants. This study comparatively evaluated the performance of the iC-GPC Assay against the Verigene® gram-positive blood culture (BC-GP) assay (Luminex Corp®, Austin, TX) for 1,134 patient blood culture specimens positive for gram-positive cocci...
June 13, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29898739/longitudinal-chemokine-profile-expression-in-a-blood-brain-barrier-model-from-alzheimer-transgenic-versus-wild-type-mice
#6
J Vérité, T Janet, D Chassaing, B Fauconneau, H Rabeony, G Page
BACKGROUND: Alzheimer's disease is widely described since the discovery of histopathological lesions in Mrs. Auguste Deter in 1906. However to date, there is no effective treatment to deal with the many cellular and molecular alterations. The complexity is even higher with the growing evidence of involvement of the peripheral blood mononuclear cells (PBMCs). Indeed, monocytes and T cells are shown in the cerebral parenchyma of AD patients, and these cells grafted to the periphery are able to go through the blood-brain barrier (BBB) in transgenic mouse models...
June 13, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29896216/mir-155-and-mir-122-expression-of-spermatozoa-in-obese-subjects
#7
Paulina López, Andrea Castro, Martha Flórez, Karen Miranda, Pilar Aranda, Cristina Sánchez-González, Juan Llopis, Miguel Arredondo
Obesity is characterized by mild chronic inflammation that is linked with impaired iron homeostasis. Studies in human and murine show that there is a transgenerational epigenetic inheritance via the gametes in obesity; however, there is little information on changes in the expression of microRNAs related to inflammation and iron homeostasis in spermatozoa from obese subjects. The present study investigated the expression of microRNAs related to inflammation (miR-21 y miR-155) and iron nutrition (miR-122 and miR-200b) in plasma, peripheral blood mononuclear cells (PBMC) and spermatozoa from normozoospermic controls (Cn; n = 17; BMI: 24...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29896203/efficacy-and-safety-of-mepolizumab-anti-interleukin-5-treatment-in-gleich-s-syndrome
#8
Andrea Matucci, Francesco Liotta, Emanuele Vivarelli, Laura Dies, Francesco Annunziato, Marie Pierre Piccinni, Francesca Nencini, Sara Pratesi, Enrico Maggi, Alessandra Vultaggio
Gleich's syndrome (GS) is characterized by recurrent episodes of angioedema, increase in body weight, fever, hypereosinophilia, and elevated serum IgM. The exact etiology remains unclear. Currently, the only treatment strategy is the administration of high dose of steroids during the acute phases. We report the case of a 37-year-old man suffering from GS with recurrent episodes of angioedema, fever, hypereosinophilia [6,000/mm3 (45%)], and high eosinophil cationic protein (ECP) (>200 μg/l), treated with oral steroids during the acute phase (prednisone 50-75 mg/day), the dose of maintenance being 25 mg/day...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29895402/burn-wounds-in-the-young-versus-the-aged-patient-display-differential-immunological-responses
#9
Angel F Farinas, Ravinder Bamba, Alonda C Pollins, Nancy L Cardwell, Lillian B Nanney, Wesley P Thayer
BACKGROUND: Individuals in the geriatric age range are more prone than younger individuals to convert their partial thickness thermal burns into full thickness injuries. We hypothesized that this often observed clinical phenomenon is strongly related to differential local injury responses mediated by the immune system. MATERIALS & METHODS: Skin samples from areas with partial thickness thermal burns were obtained during routine excision and grafting procedures between post burn days 2-6...
June 9, 2018: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/29895317/hypoxia-with-wharton-s-jelly-mesenchymal-stem-cell-coculture-maintains-stemness-of-umbilical-cord-blood-derived-cd34-cells
#10
Dewan Zhao, Lingjia Liu, Qiang Chen, Fangfang Wang, Qiuyang Li, Qiang Zeng, Jingcao Huang, Maowen Luo, Wenxian Li, Yuhuan Zheng, Ting Liu
BACKGROUND: The physiological approach suggests that an environment associating mesenchymal stromal cells with low O2 concentration would be most favorable for the maintenance of hematopoietic stem/progenitor cells (HSPCs). To test this hypothesis, we performed a coculture of cord blood CD34+ cells with Wharton's jelly mesenchymal stem cells (WJ-MSCs) under different O2 concentration to simulate the growth of HSPCs in vivo, and assessed the impacts on stemness maintenance and proliferation of cord blood HSPCs in vitro...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29891204/stereotactic-ablative-radiation-therapy-induces-systemic-differences-in-peripheral-blood-immunophenotype-dependent-on-irradiated-site
#11
Heather M McGee, Megan E Daly, Sohelia Azghadi, Susan L Stewart, Leslie Oesterich, Jeffrey Schlom, Renee Donahue, Jonathan D Schoenfeld, Qian Chen, Shyam Rao, Ruben C Fragoso, Richard K Valicenti, Robert J Canter, Emmanual M Maverakis, William J Murphy, Karen Kelly, Arta M Monjazeb
PURPOSE: Despite the strong interest in combining stereotactic ablative radiation therapy (SAR) with immunotherapy, limited data characterizing the systemic immune response after SAR are available. We hypothesized that the systemic immune response to SAR would differ by irradiated site owing to inherent differences in the microenvironment of various organs. METHODS AND MATERIALS: Patients receiving SAR to any organ underwent prospective blood banking before and 1 to 2 weeks after SAR...
April 22, 2018: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/29888837/viral-dominance-patterns-in-chronic-hepatitis-delta-determine-early-response-to-interferon-alpha-therapy
#12
Gunnar Lewon Lutterkort, Anika Wranke, Julia Hengst, Cihan Yurdaydin, Judith Stift, Birgit Bremer, Svenja Hardtke, Onur Keskin, Ramazan Idilman, Michael P Manns, Hans Peter Dienes, Christine Falk, Heiner Wedemeyer, Benjamin Heidrich
Chronic hepatitis D is caused by coinfection of hepatitis B and hepatitis D virus. While HDV is the dominant virus over HBV in the majority of cases, mechanisms and consequences of viral dominances are largely unknown. We aimed to investigate associations between viral dominance patterns and patients' characteristics and inflammatory features. 109 HDV patients treated with PEG-IFNa-2a within the international multicentre, prospective HIDIT-2 trial were studied. Patients were classified as D- or B-dominant if the viral load of one virus exceeded that of the other virus by more than 1log10 ...
June 11, 2018: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29885443/value-of-c3d-assay-and-igg-subclass-in-the-prediction-of-the-flow-cytometry-cross-match-result-for-renal-transplantation
#13
Alan G Contreras, Aurora Casillas-Abundis, Josefina Alberú, Luis Llorente, Guadalupe Lima, Adriana Arvizu, Adrián de Santiago, Mario Vilatobá, Julio Granados, Luis E Morales-Buenrostro, Rodrigo Cruz, José M Arreola-Guerra
The aim of this study is to compare the association and the predictive capacity of DSA MFI, complement fixing capacity (C3d assay) and IgG subclasses determination in the prediction of FCxM result. METHODS: We used cryopreserved (70C) sera from potential renal transplant recipients, containing DSA against their respective potential donors. All patients showed negative AHG-CDC CxM and either positive or negative FCxM. Class I and Class II HLA-DSA were determined by Luminex SAB. C3d were detected by luminex (Lifecodes®Immucor), DSA IgG 1-4 subclasses were evaluated using monoclonal antibodies specific for IgG subclasses (Luminex)...
June 6, 2018: Transplant Immunology
https://www.readbyqxmd.com/read/29879060/detection-of-common-diarrhea-causing-pathogens-in-northern-taiwan-by-multiplex-polymerase-chain-reaction
#14
Shu-Huan Huang, Yi-Fang Lin, Ming-Han Tsai, Shuan Yang, Mei-Ling Liao, Shao-Wen Chao, Cheng-Cheng Hwang
Conventional methods for identifying gastroenteritis pathogens are time consuming, more likely to result in a false-negative, rely on personnel with diagnostic expertise, and are dependent on the specimen status. Alternatively, molecular diagnostic methods permit the rapid, simultaneous detection of multiple pathogens with high sensitivity and specificity. The present study compared conventional methods with the Luminex xTAG Gastrointestinal Pathogen Panel (xTAG GPP) for the diagnosis of infectious gastroenteritis in northern Taiwan...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29877923/therapeutic-human-igg-preparations-contain-mixture-of-hla-antibodies-to-native-hla-antigens-and-cryptic-epitopes-with-little-clinical-significance
#15
Massimo Mangiola, Marilyn Marrari, Christopher Ensor, Martin O Spycher, Mel Berger, Adriana Zeevi
BACKGROUND: Human immunoglobulins (H-Ig) are widely used in solid organ transplantation for IgG replacement and for desensitization and treatment of antibody mediated rejection. They are obtained from plasma pools and may contain HLA antibodies that can be detrimental to transplant recipients. The goal of this study was to evaluate HLA antibodies in multiple lots of 2 commercial H-Ig preparations by. Luminex single antigen bead (SAB) and cell-based crossmatch assays. METHODS: Thirty lots of 2 commercial H-Ig products (CSL Behring, King of Prussia, PA...
June 6, 2018: Transplantation
https://www.readbyqxmd.com/read/29875399/networks-of-blood-proteins-in-the-neuroimmunology-of-schizophrenia
#16
Clark D Jeffries, Diana O Perkins, Margot Fournier, Kim Q Do, Michel Cuenod, Ines Khadimallah, Enrico Domenici, Jean Addington, Carrie E Bearden, Kristin S Cadenhead, Tyrone D Cannon, Barbara A Cornblatt, Daniel H Mathalon, Thomas H McGlashan, Larry J Seidman, Ming Tsuang, Elaine F Walker, Scott W Woods
Levels of certain circulating cytokines and related immune system molecules are consistently altered in schizophrenia and related disorders. In addition to absolute analyte levels, we sought analytes in correlation networks that could be prognostic. We analyzed baseline blood plasma samples with a Luminex platform from 72 subjects meeting criteria for a psychosis clinical high-risk syndrome; 32 subjects converted to a diagnosis of psychotic disorder within two years while 40 other subjects did not. Another comparison group included 35 unaffected subjects...
June 6, 2018: Translational Psychiatry
https://www.readbyqxmd.com/read/29872127/are-systemic-manifestations-ascribable-to-copd-in-smokers-a-structural-equation-modeling-approach
#17
Laurent Boyer, Sylvie Bastuji-Garin, Christos Chouaid, Bruno Housset, Philippe Le Corvoisier, Geneviève Derumeaux, Jorge Boczkowski, Bernard Maitre, Serge Adnot, Etienne Audureau
Whether the systemic manifestations observed in Chronic Obstructive Pulmonary Disease (COPD) are ascribable to lung dysfunction or direct effects of smoking is in debate. Structural Equations Modeling (SEM), a causal-oriented statistical approach, could help unraveling the pathways involved, by enabling estimation of direct and indirect associations between variables. The objectives of the study was to investigate the relative impact of smoking and COPD on systemic manifestations, inflammation and telomere length...
June 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29869602/serological-evidence-of-ebola-virus-infection-in-rural-guinea-before-the-2014-west-african-epidemic-outbreak
#18
Alpha K Keita, Christelle Butel, Guillaume Thaurignac, Aminata Diallo, Talla Nioke, Falaye Traoré, Lamine Koivogui, Martine Peeters, Eric Delaporte, Ahidjo Ayouba
Questions remain as whether an unnoticed Ebola outbreak has occurred in Guinea before the 2014-2016 epidemic. To address this, we used a highly sensitive and specific Luminex-based assay for Ebola virus (EBOV) antibody detection to screen blood samples collected in the framework of the Demographic Health Survey performed in 2012 in Guinea. One sample (GF069) of 1,483 tested was positive at very high immunoglobulin G titer to Zaire EBOV in Guinée Forestière. Thus, at least 2 years before the 2014 EVD outbreak in Guinea, Zaire EBOV was circulating in rural areas of this country...
June 4, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29852336/cytokine-profiles-of-women-with-vulvodynia-identification-of-a-panel-of-pro-inflammatory-molecular-targets
#19
Nunzia Zanotta, Giuseppina Campisciano, Federica Scrimin, Ura Blendi, Annalisa Marcuzzi, Ezio Vincenti, Sergio Crovella, Manola Comar
OBJECTIVE: The vulvar pain syndrome (VPS) is a multifactorial disease severely influencing the lifestyle of affected women. Among possible etiological factors, local injury, peripheral and/or central sensitization of the nervous system, and a chronic inflammatory status have been positively associated with the development of VPS. The identification of a constitutive altered local inflammatory profile in VPS women may represent an important point in the characterization of patients' phenotype as a useful marker influencing the vulvar micro-environment...
July 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29849593/evaluating-il-21-as-a-potential-therapeutic-target-in-crohn-s-disease
#20
Thomas Lindebo Holm, Ditte Tornehave, Henrik Søndergaard, Peter Helding Kvist, Bodil-Cecilie Sondergaard, Lene Hansen, Mette Brunsgaard Hermit, Kristine Holgersen, Sandra Vergo, Klaus Stensgaard Frederiksen, Claus Haase, Dorthe Lundsgaard
Background and Aim: Interleukin-21 (IL-21) is primarily a T cell-derived cytokine; it is upregulated in patients with Crohn's Disease (CD) and could be a potential new therapeutic target in CD. Methods: In human material, IL-21 and IL-21R expression was investigated by in situ hybridization (ISH) and immunohistochemistry (IHC) in noninflammatory bowel disease (non-IBD) controls and patients with CD. The pathologic role of IL-21 was examined in murine models of T cell-dependent and T cell-independent colitis, either with a neutralizing monoclonal antibody against IL-21 or with the transfer of CD4+ CD45RBhigh IL-21R-/- T cells...
2018: Gastroenterology Research and Practice
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