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https://www.readbyqxmd.com/read/29676812/impact-of-pretransplant-donor-specific-antibodies-on-kidney-allograft-recipients-with-negative-flow-cytometry-crossmatches
#1
Hyunwook Kwon, Young Hoon Kim, Ji Yoon Choi, Sung Shin, Joo Hee Jung, Su-Kil Park, Duck Jong Han
The Luminex test can detect low levels of donor-specific antibody (DSA) that cannot be detected by flow-cytometric crossmatching (FCXM) in kidney transplantation (KT). This study evaluated the impact of DSA on clinical outcomes in KT recipients negative on FCXM. Of 575 consecutive patients who underwent living donor KT between January 2013 and July 2016, 494 (85.9%) were DSA-negative and 81 (14.1%) were DSA-positive. Although rates of acute cellular rejection (ACR) at 1 year were similar in the two groups (P = 0...
April 20, 2018: Clinical Transplantation
https://www.readbyqxmd.com/read/29673332/expression-of-cytokines-in-aqueous-humor-from-fungal-keratitis-patients
#2
Yingnan Zhang, Qingfeng Liang, Yang Liu, Zhiqiang Pan, Christophe Baudouin, Antoine Labbé, Qingxian Lu
BACKGROUND: Although a series of reports on corneal fungal infection have been published, studies on pathogenic mechanisms and inflammation-associated cytokines remain limited. In this study, aqueous humor samples from fungal keratitis patients were collected to examine cytokine patterns and cellular profile for the pathogenesis of fungal keratitis. METHODS: The aqueous humor samples were collected from ten patients with advanced stage fungal keratitis. Eight aqueous humor samples from patients with keratoconus or corneal dystrophy were taken as control...
April 19, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/29669260/limited-impact-of-pre-existing-donor-specific-hla-antibodies-dsa-on-long-term-allograft-survival-after-first-adult-liver-transplantation
#3
Martina Koch, Matthias Marget, Martina Sterneck, Lutz Fischer, Hansjörg Thude, Björn Nashan
The relevance of pre-existing HLA-antibodies (HLA-Ab) in liver transplantation (LTx) is controversial. While livers are allocated without HLA match or preoperative crossmatch testing, several studies point to an impact of donor specific antibodies (DSA) for acute rejection, bile duct complications or even graft loss. To investigate the role of DSA in long term liver allograft survival we analysed 177 pre transplant sera of first LTx patients with luminex single antigen technology defining a MFI of >1500 as positive...
April 15, 2018: Human Immunology
https://www.readbyqxmd.com/read/29662241/low-mother-to-child-ccl22-chemokine-levels-are-inversely-related-to-mite-sensitization-and-asthma-in-early-childhood
#4
Chih-Yung Chiu, Kuan-Wen Su, Ming-Han Tsai, Man-Chin Hua, Sui-Ling Liao, Shen-Hao Lai, Li-Chen Chen, Tsung-Chieh Yao, Kuo-Wei Yeh, Jing-Long Huang
Few studies have addressed the mother-to-child transmission of Th2 immunity and the impact on the development of atopic diseases in early childhood. We investigated 186 children who were followed-up regularly for 4 years in a birth cohort study. The levels of Th2 related chemokine (C-C motif) ligand 17 (CCL17) and CCL22 were quantified in cord blood and at 1.5 years-of-age using multiplex Luminex kits. The levels of 125 pairs of CCL17 and CCL22 chemokines from birth to 1.5 years were recorded in this study...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29660489/prevalence-of-mucosal-and-cutaneous-human-papillomavirus-in-moroccan-breast-cancer
#5
Amal ElAmrani, Tarik Gheit, Mustapha Benhessou, Sandrine McKay-Chopin, Mohammed Attaleb, Souha Sahraoui, Mohammed El Mzibri, Marilys Corbex, Massimo Tommasino, Meriem Khyatti
BACKGROUND: Due to recent technical improvements and some encouraging new results, there has been a resurgence of interest in the possibility that a substantial proportion of breast cancers (BCs) may be caused by viral infections, including Human papillomavirus (HPV). The aim of this study was to determine the prevalence of mucosal and cutaneous HPV in tumours from Moroccan BC patients. MATERIALS AND METHODS: Frozen tumours from 76 BC cases and 12 controls were evaluated for the presence of 62 HPV-types using highly sensitive assays that combine multiplex polymerase chain reaction and bead-based Luminex technology...
April 13, 2018: Papillomavirus Research
https://www.readbyqxmd.com/read/29659574/the-impact-of-school-water-sanitation-and-hygiene-improvements-on-infectious-disease-using-serum-antibody-detection
#6
Anna N Chard, Victoria Trinies, Delynn M Moss, Howard H Chang, Seydou Doumbia, Patrick J Lammie, Matthew C Freeman
BACKGROUND: Evidence from recent studies assessing the impact of school water, sanitation and hygiene (WASH) interventions on child health has been mixed. Self-reports of disease are subject to bias, and few WASH impact evaluations employ objective health measures to assess reductions in disease and exposure to pathogens. We utilized antibody responses from dried blood spots (DBS) to measure the impact of a school WASH intervention on infectious disease among pupils in Mali. METHODOLOGY/PRINCIPAL FINDINGS: We randomly selected 21 beneficiary primary schools and their 21 matched comparison schools participating in a matched-control trial of a comprehensive school-based WASH intervention in Mali...
April 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29656317/inflammatory-response-of-pulmonary-artery-smooth-muscle-cells-exposed-to-oxidative-and-biophysical-stress
#7
Joanna Costa, Yan Zhu, Timothy Cox, Paul Fawcett, Thomas Shaffer, Deepthi Alapati
Pulmonary hypertension in the neonate requires treatment with oxygen and positive pressure ventilation, both known to induce lung injury. The direct response of pulmonary artery smooth muscle cells, the most abundant cells in the artery wall, to the stress of positive pressure and hyperoxia has not been previously studied. Pulmonary artery smooth muscle cells were cultured in temperature- and pressure-controlled air-tight chambers under conditions of positive pressure or hyperoxia for 24 h. Control cells were cultured in room air under atmospheric pressure...
April 14, 2018: Inflammation
https://www.readbyqxmd.com/read/29643194/quantitative-thresholds-enable-accurate-identification-of-clostridium-difficile-infection-by-the-luminex-xtag-gi-pathogen-panel
#8
Sixto M Leal, Elena B Popowitch, Kara J Levinson, Teny M John, Bethany Lehman, Maria Bueno Rios, Peter H Gilligan, Melissa B Miller
Clostridium difficile colonizes the gastrointestinal tract resulting in either asymptomatic carriage or a spectrum of diarrheal illness. If clinical suspicion for C. difficile is low, stool samples are often submitted for analysis by multiplex molecular assays capable of detecting multiple GI pathogens and some institutions do not report this organism due to concerns for high false positive rates. Since clinical disease correlates with organism burden and molecular assays yield quantitative data, we hypothesized that numerical cutoffs could be utilized to improve the specificity of the Luminex xTAG GI pathogen panel (GPP) for C...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29625588/rapid-detection-of-three-rabbit-pathogens-by-use-of-the-luminex-x-tag-assay
#9
Miaoli Wu, Yujun Zhu, Feng Cong, Dan Rao, Wen Yuan, Jing Wang, Bihong Huang, Yuexiao Lian, Yu Zhang, Ren Huang, Pengju Guo
BACKGROUND: Domestic rabbits especially New Zealand white rabbits play an important role in biological research. The disease surveillance and quality control are essential to guarantee the results of animal experiments performed on rabbits. Rabbit hemorrhagic disease virus, rabbit rotavirus and Sendai virus are the important pathogens that needed to be eliminated. Rapid and sensitive method focus on these three viruses should be established for routine monitoring. The Luminex x-TAG assay based on multiplex PCR and fluorescent microsphere is a fast developing technology applied in high throughput detection...
April 7, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29620542/donor-specific-hla-dq-antibodies-may-contribute-to-poor-graft-outcome-after-heart-transplantation
#10
Osama Omrani, Moheeb Alawwami, Jehad Buraiki, Nedim Selimovic
BACKGROUND: HLA-DQ donor-specific antibodies (DSA) are implicated in allograft dysfunction after renal and lung transplantation. Limited data exists on the impact of HLA-DQ antibodies on heart transplant patients. OBJECTIVE: To investigate the impact of DSA formation on allograft function and outcomes in heart transplant patients. DESIGN: Retrospective cohort study. SETTING: Collating post-transplantation patient data from computerized database in a tertiary hospital in Riyadh, Saudi Arabia from January 2006 to October 2014...
March 2018: Annals of Saudi Medicine
https://www.readbyqxmd.com/read/29617399/activation-of-human-macrophages-by-human-corneal-allogen-in-vitro
#11
Paola Kammrath Betancor, Antonia Hildebrand, Daniel Böhringer, Florian Emmerich, Günther Schlunck, Thomas Reinhard, Thabo Lapp
PURPOSE: To study distinct aspects of human monocyte-derived macrophage (MDM) activation by human corneal tissue as a possible initial stage in human corneal allograft rejection. METHODS: Human monocytes were isolated from peripheral blood mononuclear cells (PBMC) and differentiated into MDM. Human corneas with or without endothelium were fragmented using a standardized protocol. MDM were stimulated with human corneal fragments, corneal fragment supernatant, lipopolysaccharide (LPS) or interferon-gamma (IFNγ), and expression profiles for 34 cytokines were determined in MDM-conditioned media using a Luminex bead-based multiplex assay...
2018: PloS One
https://www.readbyqxmd.com/read/29616012/establishment-of-a-molecular-serotyping-scheme-and-a-multiplexed-luminex-based-array-for-enterobacter-aerogenes
#12
Xi Guo, Min Wang, Lu Wang, Yao Wang, Tingting Chen, Pan Wu, Min Chen, Bin Liu, Lu Feng
Serotyping based on surface polysaccharide antigens is important for the clinical detection and epidemiological surveillance of pathogens. Polysaccharide gene clusters (PSgcs) are typically responsible for the diversity of bacterial surface polysaccharides. Through whole-genome sequencing and analysis, eight putative PSgc types were identified in 23 Enterobacter aerogenes strains from several geographic areas, allowing us to present the first molecular serotyping system for E. aerogenes . A conventional antigenic scheme was also established and correlated well with the molecular serotyping system that was based on PSgc genetic variation, indicating that PSgc-based molecular typing and immunological serology provide equally valid results...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29615087/development-of-a-sensitive-luminex-xmap-based-microsphere-immunoassay-for-specific-detection-of-iris-yellow-spot-virus
#13
Cui Yu, Cuiyun Yang, Shaoyi Song, Zixiang Yu, Xueping Zhou, Jianxiang Wu
BACKGROUND: Iris yellow spot virus (IYSV) is an Orthotospovirus that infects most Allium species. Very few approaches for specific detection of IYSV from infected plants are available to date. We report the development of a high-sensitive Luminex xMAP-based microsphere immunoassay (MIA) for specific detection of IYSV. RESULTS: The nucleocapsid (N) gene of IYSV was cloned and expressed in Escherichia coli to produce the His-tagged recombinant N protein. A panel of monoclonal antibodies (MAbs) against IYSV was generated by immunizing the mice with recombinant N protein...
April 4, 2018: Virology Journal
https://www.readbyqxmd.com/read/29614665/blood-inflammatory-mediators-and-cognitive-decline-in-alzheimer-s-disease-a-two-years-longitudinal-study
#14
Adrien Julian, Agnès Rioux-Bilan, Stéphanie Ragot, Pierre Krolak-Salmon, Gilles Berrut, Thierry Dantoine, Caroline Hommet, Olivier Hanon, Guylène Page, Marc Paccalin
Peripheral inflammatory processes are involved in Alzheimer's disease (AD). We aimed to determine whether plasma inflammatory mediator levels at diagnosis are associated with cognitive decline through a 2-year follow-up in AD patients. Patients (n = 109, mean age 79.44 (6.82) years) were included at diagnosis with MMSE scores between 16 and 25, with C-reactive protein <10 mg/L, and without any acute or chronic inflammation status. Plasma IL-1β, IL-6, TNF-α, and CCL5 were measured using Luminex X-MAP technology at baseline, and after one year and two years of follow-up...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29614603/-serotype-distribution-and-antimicrobial-resistance-of-salmonella-isolates-from-retail-chicken-carcasses-in-six-provinces-of-china
#15
Y J Hu, Y Y He, Y R Wang, C Liu, M M Wang, X Gan, W Wang, S F Yan, Y Bai, Z X Peng, F Q Li, J Xu
Objective: To obtain the serotype diversity and antimicrobial resistance of Salmonella isolates recovered from retail chicken carcasses for sale in six regions of China. Methods: From August 2010 to March 2012, each month 20 retail chicken carcasses including freshly slaughtered, chilled and frozen samples were collected from supermarkets and farmer's markets in 7 monitoring sites in Beijing, Jilin province, Inner Mongolia Autonomous, Shanxi province, Jiangsu province and Guangdong province, respectively. Samples were routinely collected for 12 months for each site...
April 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29604263/cerebrospinal-fluid-%C3%AE-synuclein-contributes-to-the-differential-diagnosis-of-alzheimer-s-disease
#16
Min Shi, Lu Tang, Jon B Toledo, Carmen Ginghina, Hua Wang, Patrick Aro, Poul H Jensen, Daniel Weintraub, Alice S Chen-Plotkin, David J Irwin, Murray Grossman, Leo McCluskey, Lauren B Elman, David A Wolk, Edward B Lee, Leslie M Shaw, John Q Trojanowski, Jing Zhang
INTRODUCTION: The ability of Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers (amyloid β peptide 1-42, total tau, and phosphorylated tau) to discriminate AD from related disorders is limited. Biomarkers for other concomitant pathologies (e.g., CSF α-synuclein [α-syn] for Lewy body pathology) may be needed to further improve the differential diagnosis. METHODS: CSF total α-syn, phosphorylated α-syn Ser129, and AD CSF biomarkers were evaluated with Luminex immunoassays in 367 participants, followed by validation in 74 different neuropathologically confirmed cases...
March 28, 2018: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
https://www.readbyqxmd.com/read/29601268/influence-of-a-concurrent-exercise-training-program-during-pregnancy-on-colostrum-and-mature-human-milk-inflammatory-markers-findings-from-the-gestafit-project
#17
Virginia A Aparicio, Olga Ocón, Javier Diaz-Castro, Pedro Acosta-Manzano, Irene Coll-Risco, Milkana Borges-Cósic, Lidia Romero-Gallardo, Jorge Moreno-Fernández, Julio J Ochoa-Herrera
BACKGROUND: Although exercise reduces systemic inflammation, information regarding its influence on human milk is scarce or inexistent. Research Aim: The aim of this study was to investigate the influence of an exercise intervention during pregnancy on colostrum and mature human milk inflammatory markers. METHODS: The authors conducted a pseudorandomized controlled trial. The exercise group followed a concurrent aerobic and strength training, three 60-minutes sessions per week, from the 17th gestational week until delivery...
March 1, 2018: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/29600444/serum-ykl-40-as-a-biomarker-for-liver-fibrosis-in-chronic-hepatitis-b-patients-with-normal-and-mildly-elevated-alt
#18
Linlin Yan, Yongqiong Deng, Jiyuan Zhou, Hong Zhao, Guiqiang Wang
PURPOSE: YKL-40 is a chitinase-like protein expressed in multiple tissues including liver and is reported as a fibrosis marker. This study aimed to determine whether YKL-40 could serve as a diagnostic marker for the assessment of liver fibrosis in chronic hepatitis B patients with normal and mildly elevated ALT. METHODS: Six hundred and eighty-five patients with chronic hepatitis B infection were enrolled in this study from October 2013 to March 2016. All patients underwent liver biopsy and then staged based on Ishak histological system...
March 29, 2018: Infection
https://www.readbyqxmd.com/read/29599686/alignments-of-endocrine-anthropometric-and-metabolic-parameters-in-type-2-diabetes-after-intervention-with-an-okinawa-based-nordic-diet
#19
Bodil Ohlsson, Gassan Darwiche, Bodil Roth, Peter Höglund
Background: An Okinawa-based Nordic diet with moderately low carbohydrate content and high fat and protein content has been shown to improve anthropometry and metabolism in type 2 diabetes. Objective: The objectives of this study were to measure plasma or serum levels of hormones regulating energy metabolism and metabolic control, that is, cholecystokinin (CCK), Cortisol, C-peptide, ghrelin, glucagon, glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP), insulin, leptin, plasminogen activator inhibitor-1 (PAI-1), polypeptide YY (PYY), resistin, and visfatin after this diet intervention, and to determine partial correlations between hormonal levels and anthropometric and metabolic responses...
2018: Food & Nutrition Research
https://www.readbyqxmd.com/read/29580160/significant-transcriptome-and-cytokine-changes-in-hepatitis-b-vaccine-non-responders-revealed-by-genome-wide-comparative-analysis
#20
Shaohui Qiu, Peng He, Xin Fang, Haiqing Tong, Jingjing Lv, Jiaye Liu, Li Zhang, Xiangjun Zhai, Liying Wang, Zhongyu Hu, Yongli Yu
Individuals fail to elicit protective antibody after hepatitis B vaccination remain at risk for hepatitis B virus infection. Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) is essential to elucidate the characteristics of gene expression in non-responders. In this study, we enrolled seven responders who had received three injections and seven non-responders who had six injections of hepatitis B vaccine before. All the participants were then vaccinated with a three-dose boost regimen...
March 26, 2018: Human Vaccines & Immunotherapeutics
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