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https://www.readbyqxmd.com/read/28231571/luminex-liquichip-system-for-the-evaluation-of-cytokine-levels-in-patients-with-contact-dermatitis-to-potassium-dichromate
#1
Lehavit Akerman, Tirza Klein, Moshe Israeli, Abigail Fraser, Eti Sagy, Igor Snast, Daniel Mimouni, Michael David, Akiva Trattner
BACKGROUND: Allergic contact dermatitis (ACD) is associated with increased production of cytokines. The patch test is the "gold-standard" diagnostic method, but it poses a risk of false results. OBJECTIVE: To evaluate a novel laboratory technique, the Luminex LiquiChip, which simultaneously measures blood levels of multiple cytokines, as a diagnostic tool in patients with chrome-induced ACD. METHODS: The study group included 20 patients with ACD and relevant patch test results for potassium dichromate and 19 patients with ACD for nickel or fragrance as control...
February 24, 2017: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/28228489/age-dependent-shift-in-macrophage-polarisation-causes-inflammation-mediated-degeneration-of-enteric-nervous-system
#2
Laren Becker, Linh Nguyen, Jaspreet Gill, Subhash Kulkarni, Pankaj Jay Pasricha, Aida Habtezion
OBJECTIVE: The enteric nervous system (ENS) undergoes neuronal loss and degenerative changes with age. The cause of this neurodegeneration is poorly understood. Muscularis macrophages residing in close proximity to enteric ganglia maintain neuromuscular function via direct crosstalk with enteric neurons and have been implicated in the pathogenesis of GI motility disorders like gastroparesis and postoperative ileus. The aim of this study was to assess whether ageing causes alterations in macrophage phenotype that contributes to age-related degeneration of the ENS...
February 21, 2017: Gut
https://www.readbyqxmd.com/read/28226272/automated-processing-extraction-and-detection-of-herpes-simplex-virus-types-1-and-2-a-comparative-evaluation-of-three-commercial-platforms-using-clinical-specimens
#3
Matthew J Binnicker, Mark J Espy, Brian Duresko, Cole Irish, Jay Mandrekar
BACKGROUND: Recently, automated platforms have been developed that can perform processing, extraction and testing for herpes simplex virus (HSV) nucleic acid on a single instrument. OBJECTIVES: In this study, we compared three commercially-available systems; Aptima(®)/Panther (Hologic, San Diego, CA), ARIES(®) (Luminex Corporation, Austin, TX), and cobas(®) 4800 (Roche Molecular Systems Inc, Pleasanton, CA) for the qualitative detection of HSV-1/2 in clinical samples...
February 14, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28225833/influence-of-hla-class-i-hla-class-ii-and-kirs-on-vertical-transmission-and-chronicity-of-hepatitis-c-virus-in-children
#4
A Ruiz-Extremera, E J Pavón-Castillero, M Florido, P Muñoz de Rueda, J A Muñoz-Gámez, J Casado, A Carazo, R Quiles, S M Jiménez-Ruiz, A Gila, J D Luna, J León, J Salmerón
BACKGROUND & AIM: There is evidence that maternal viral load of HCV during delivery influences the risk for Mother-to-child transmission (MTCT), but this does not explain all cases. We study the role of the immunogenetic profile (HLA, KIRs and KIR-ligand binding) of mothers and children in HCV-MTCT and in chronicity in the children. METHODOLOGY: 79 HCV-RNA (+) mothers and their 98 children were included. 24 children were infected, becoming chronic in 8 cases and clearing in 16...
2017: PloS One
https://www.readbyqxmd.com/read/28225519/a-cytokine-pattern-that-differentiates-preseroconversion-from-postseroconversion-phases-of-primary-hiv-infection
#5
Lucia Pastor, Erica Parker, Jorge Carrillo, Victor Urrea, Laura Fuente-Soro, Durval Respeito, Chenjerai Jairoce, Inacio Mandomando, Julià Blanco, Denise Naniche
BACKGROUND: During acute HIV infection, HIV actively replicates but seroconversion has not yet occurred. Primary HIV infection (PHI) is characterized by a transient nonspecific febrile illness, a massive inflammatory response, and the progressive appearance of anti-HIV-specific antibodies. In this study, we have identified patterns of inflammatory biomarkers associated with the innate immunological reaction before completion of a full humoral response. METHODS: A symptom-based screening was used to identify acute HIV infection in the Manhiça District Hospital in Mozambique...
April 1, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28224774/comparison-of-luminex-nxtag-respiratory-pathogen-panel-and-xtag-respiratory-viral-panel-fast-version-2-for-the-detection-of-respiratory-viruses
#6
Chun Kiat Lee, Hong Kai Lee, Christopher Wei Siong Ng, Lily Chiu, Julian Wei Tze Tang, Tze Ping Loh, Evelyn Siew Chuan Koay
Owing to advancements in molecular diagnostics, recent years have seen an increasing number of laboratories adopting respiratory viral panels to detect respiratory pathogens. In December 2015, the NxTAG respiratory pathogen panel (NxTAG RPP) was approved by the United States Food and Drug Administration. We compared the clinical performance of this new assay with that of the xTAG respiratory viral panel (xTAG RVP) FAST v2 using 142 clinical samples and 12 external quality assessment samples. Discordant results were resolved by using a laboratory-developed respiratory viral panel...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224132/dataset-of-longitudinal-analysis-of-tear-cytokine-levels-cd4-cd8-counts-and-hiv-viral-load-in-dry-eye-patients-with-hiv-infection
#7
Praveen Kumar Balne, Rupesh Agrawal, Veonice Bijin Au, Bernett Lee, Eileen Loo, Louis Tong, Arkasubhra Ghosh, Stephen C Teoh, John Connolly, Petrina Tan
The data presented in this article shows the longitudinal analysis of tear fluid cytokine profiles, blood CD4 and CD8 counts and HIV viral load in 34 dry eye patients with HIV infection during the HAART therapy. Clinical samples were collected from HIV patients with dry eye disease at the time of presentation to the clinic (visit 1), three months (visit 2) and 6 months (visit 3) after the presentation. At each time point tear samples were evaluated for 41 cytokines using Luminex bead based multiplex assay and blood samples were tested for HIV viral load and CD4 and CD8 counts...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28222797/melanoma-antigen-specific-effector-t-cell-cytokine-secretion-patterns-in-patients-treated-with-ipilimumab
#8
Yana G Najjar, Fei Ding, Yan Lin, Robert VanderWeele, Lisa H Butterfield, Ahmad A Tarhini
BACKGROUND: In a previously reported study, patients with regionally advanced melanoma were treated with neoadjuvant ipilimumab (ipi) (Tarhini in PLoS ONE 9(2): e87705, 3). Significant changes in circulating myeloid derived suppressor cells (MDSC), regulatory T cells (Treg) and peptide specific type I CD4(+) and CD8(+) T cells were noted at week 6 that correlated with clinical outcome. Characterization of antigen-specific effector T cell secreted cytokines may shed insights into ipi associated T cell activation and function...
February 21, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28221928/rapid-identification-of-shiga-toxin-producing-escherichia-coli-o-serogroups-from-fresh-produce-and-raw-milk-enrichment-cultures-by-luminex-bead-based-suspension-array
#9
Julie A Kase, Anna Maounounen-Laasri, Andrew Lin
The U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) Chapter 4a describes a Luminex microbead-based suspension array used to screen colonies for 11 clinically relevant Shiga toxin-producing Escherichia coli (STEC) serogroups: O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157. We evaluated the usefulness of this method to identify STEC-positive enrichment samples before agar plating. Twelve E. coli strains were added to three types of fresh produce (bagged baby spinach, alfalfa sprouts, and cilantro) at levels near the detection limit of the test...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28220766/immunological-biomarkers-and-long-term-graft-survival-prospective-follow-up-of-457-kidney-transplant-recipients
#10
Michał Ciszek, Krzysztof Mucha, Bartosz Foroncewicz, Dorota Żochowska, Maciej Kosieradzki, Tadeusz Grochowiecki, Magdalena Durlik, Andrzej Górski, Leszek Pączek
INTRODUCTION    Antibodies against donor`s human leukocyte antigens (HLA) play a significant role in the pathogenesis of antibody mediated rejection, although their role during the late post-transplant period is unknown. A non-HLA polymorphic antigenic system like major histocompatibility class I chain-related antigen A (MICA) might be another target for antibody responses involved in rejection. OBJECTIVES    We conducted a 7-year prospective study to determine the effect of positivity for anti-HLA and anti-MICA antibodies on kidney graft survival...
February 15, 2017: Polskie Archiwum Medycyny Wewnętrznej
https://www.readbyqxmd.com/read/28220270/multiplex-assessment-of-serum-cytokine-and-chemokine-levels-in-idiopathic-morphea-and-vitamin-k1-induced-morphea
#11
Lori Ann Cox, Guy F Webster, Sonsoles Piera-Velazquez, Sergio A Jimenez
The levels of 63 cytokines, chemokines, and growth factors were measured in the serum of four patients with idiopathic morphea and of one patient with vitamin K1-induced morphea employing a multiplex assay to identify the role of inflammatory/immunologic events in their pathogenesis. Full-thickness skin biopsies of affected skin were analyzed by histopathology. Luminex assays for 63 cytokines, chemokines, and growth factors were performed in the sera from four patients with idiopathic morphea and in two different samples of serum obtained in two separate occasions from one patient with vitamin K1-induced morphea...
February 20, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28216437/extracellular-eosinophilic-traps-in-association-with-staphylococcus-aureus-at-the-site-of-epithelial-barrier-defects-in-severe-airway-inflammation
#12
Elien Gevaert, Nan Zhang, Olga Krysko, Feng Lan, Gabriële Holtappels, Natalie De Ruyck, Hans Nauwynck, Shida Yousefi, Hans-Uwe Simon, Claus Bachert
BACKGROUND: Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a Th2-biased eosinophilic inflammation. Eosinophils have been shown to generate so-called extracellular eosinophilic traps (EETs) under similar pathological conditions. OBJECTIVE: Our aim was to investigate a possible link between EET formation and the presence of Staphylococcus aureus, an organism frequently colonizing the upper airways, at the human mucosal site of the disease. METHODS: Tissue slides were investigated for the presence of EETs and S...
February 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28207826/changes-of-plasma-cytokines-and-chemokines-expression-level-in-nasopharyngeal-carcinoma-patients-after-treatment-with-definitive-intensity-modulated-radiotherapy-imrt
#13
Ya-Bin Jin, Guo-Yi Zhang, Kai-Rong Lin, Xiang-Ping Chen, Jin-Huan Cui, Yue-Jian Wang, Wei Luo
BACKGROUND: Potential clinical application values of certain cytokines and chemokines that participate in the process of tumor growth, invasion, and metastasis have been reported. However, there still lack of biomarkers for a great many of malignancy. This study identified cytokines or chemokines involved in the occurrence and development of nasopharyngeal carcinoma (NPC), which might be a biomarker for noninvasive early diagnosis. METHODS: The plasma levels of 19 cytokines and chemokines were detected by the luminex liquid array-based multiplexed immunoassays in 39 NPC patients before and after treatment by definitive intensity-modulated radiotherapy (IMRT)...
2017: PloS One
https://www.readbyqxmd.com/read/28203105/association-of-fatigue-and-depression-with-circulating-levels-of-proinflammatory-cytokines-and-epidermal-growth-factor-receptor-ligands-a-correlative-study-of-a-placebo-controlled-fatigue-trial
#14
Tyvin Rich, Fengmin Zhao, Ricardo A Cruciani, David Cella, Judith Manola, Michael J Fisch
CONTEXT: The biology of fatigue and depression in cancer patients is poorly understood. Hypotheses regarding cytokines and growth factors related to sickness behavior and disruption of circadian signaling have been proposed. OBJECTIVES: We prospectively examined proinflammatory cytokines (e.g., sickness behavior model) and epidermal growth factor receptor (EGFR) ligands (e.g., circadian disruption model) in the serum of cancer patients enrolled in a clinical trial testing levocarnitine for fatigue...
2017: Cancer Management and Research
https://www.readbyqxmd.com/read/28199413/low-prevalence-of-human-papillomavirus-in-head-and-neck-squamous-cell-carcinoma-in-the-northwest-region-of-the-philippines
#15
Pia Marie Albano, Dana Holzinger, Christianne Salvador, Jose Orosa, Sheryl Racelis, Modesty Leaño, Danilo Sanchez, Lara Mae Angeles, Gordana Halec, Markus Schmitt, John Donnie Ramos, Michael Pawlita
BACKGROUND: Geographic heterogeneity of human papillomavirus (HPV) involvement in head and neck squamous cell carcinoma (HNSCC) has been observed over the last few years. This trend has not been evaluated in the Philippines. Hence, this study aims to provide for the first time a data on the prevalence of HPV in HNSCC in the northwestern region of the Philippines. METHODS: Two hundred one (201) biopsy samples (179 formalin fixed paraffin embedded and 22 fresh frozen) from 163 Filipino HNSCC cases (oral cavity = 88; larynx = 60; oropharynx = 15) diagnosed between 2003 to 2013 were initially included in this study...
2017: PloS One
https://www.readbyqxmd.com/read/28187734/the-potential-neuroprotective-role-of-a-histone-deacetylase-inhibitor-sodium-butyrate-after-neonatal-hypoxia-ischemia
#16
Joanna Jaworska, Malgorzata Ziemka-Nalecz, Joanna Sypecka, Teresa Zalewska
BACKGROUND: Histone deacetylase inhibitor (HDACi), sodium butyrate (SB), has been shown to be neuroprotective in adult brain injury models. Potential explanation for the inhibitor action involves among others reduced inflammation. We therefore anticipated that SB will provide a suitable option for brain injury in immature animals. The aim of our study was to test the hypothesis that one of the mechanisms of protection afforded by SB after neonatal hypoxia-ischemia is associated with anti-inflammatory action...
February 10, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28186591/-application-of-recombinant-gp%C3%A2-a-combined-luminex-beads-for-the-detection-of-hpa-1a-antibody
#17
Sudan Tao, Ying Liu, Yanming He, Yanling Ying, Ji He, Faming Zhu
OBJECTIVE: To generate recombinant GPⅢa as an alternative source for HPA-1a antigen and combine it with Luminex xMAP beads for the detection of HPA-1a-specific alloantibody. METHODS: The full coding region of ITGB3 gene was amplified and ligated with pcDNA3.1. The recombinant plasmid was transfected into CHO cells, and those with stable expression were screened with G418. Expressed protein was identified and coupled with Luminex xMAP beads, which were then reacted with sera samples...
February 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28185837/insulin-promotes-her2-signaling-activation-during-barrett-s-esophagus-carcinogenesis
#18
Diletta Arcidiacono, Alessandro Antonello, Matteo Fassan, Daniele Nucci, Tiziana Morbin, Marco Agostini, Donato Nitti, Massimo Rugge, Alfredo Alberti, Giorgio Battaglia, Stefano Realdon
BACKGROUND: Insulin-resistance and hyperinsulinemia could have a role in the growing incidence of esophageal adenocarcinoma (EAC) and its pre-cancerous lesion Barrett's Esophagus (BE). HER2 activation has also a pivotal role in EAC carcinogenesis but no data correlate these two phenomena in this disease context. AIMS: To investigate the role of hyperinsulinemia in BE-dysplasia-adenocarcinoma sequence and the possible relationship between insulin-mediated and HER2 signaling in EAC development...
January 22, 2017: Digestive and Liver Disease
https://www.readbyqxmd.com/read/28184239/randomized-trial-evaluating-self-sampling-for-hpv-dna-based-tests-for-cervical-cancer-screening-in-nigeria
#19
Fatima Modibbo, K C Iregbu, James Okuma, Annemiek Leeman, Annemieke Kasius, Maurits de Koning, Wim Quint, Clement Adebamowo
BACKGROUND: Cervical cancer incidence and mortality rates in Sub-Saharan Africa (SSA) remain high due to several factors including low levels of uptake of cervical cancer screening. Self-collection of cervicovaginal samples for HPV DNA testing may be an effective modality that can increase uptake of cervical cancer screening in SSA and hard to reach populations in developed countries. We investigated whether self-collection of cervicovaginal samples for HPV DNA tests would be associated with increased uptake of screening compared with clinic based collection of samples...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28177709/effects-of-resistance-training-and-estrogen-replacement-on-adipose-tissue-inflammation-in-ovariectomized-rats
#20
Maria Fernanda Cury Rodrigues, Fabiano Candido Ferreira, Natália Santanielo Silva-Magosso, Marina Rodrigues Barbosa, Markus Vinicius Campos Souza, Mateus Moraes Domingos, Gustavo Henrique Rigo Canevazzi, Uliana Sbeguen Stotzer, Sabrina Messa Peviani, Fábio Santos de Lira, Heloísa Sobreiro Selistre de Araújo, Sérgio Eduardo de Andrade Perez
Estrogen deficiency is directly related to central obesity and low-grade inflammation. Hormonal replacement and exercise training are both able to decrease fat accumulation and inflammation in postmenopausal women. However, the efficiency of resistance training (RT) and estrogen replacement (ER) in minimizing adiposity and inflammation in the visceral adipose tissue (VAT) of ovariectomized (OVX) rats has not yet been elucidated. In this study, Sprague-Dawley rats were divided into the following six groups: the sham-operated sedentary (Sham-Sed), ovariectomized sedentary (OVX-Sed), sham-operated resistance training (Sham-RT), OVX-RT, OVX-Sed-ER and OVX-RT-ER groups...
January 25, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
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