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miRNA expression profiling

Zahra Hatefi, Goljahan Soltani, Sharifeh Khosravi, Mohammad Kazemi, Ahmad Reza Salehi, Rasoul Salehi
Background: The relationship between dyslipidemia and type 2 diabetes mellitus (T2DM) has been frequently reported. Lipoprotein lipase (LPL) is considered to be an effective gene in regulating lipid profile. MicroRNAs (miRNAs) are small noncoding RNAs involved in posttranscriptional regulation of gene expression. In the present study, we have evaluated rs13702 (C/T) polymorphism located in miRNA-410 binding site of LPL gene in subset of Iranian T2DM patients and their normal counterparts...
2018: Advanced Biomedical Research
Chia-Wei Lin, Ming-Shiou Jan, Jung-Hua Steven Kuo
Intercellular communication is known to be involved in various stages of tumor development and metastasis through the secretion of extracellular vesicles (EVs) containing messengers such as microRNAs (miRNAs). Therefore, this study explored miRNA profiles in cancer cell-derived EVs after non-viral gene delivery in order to better understand the molecular information of intercellular communication in cancer cells after gene delivery. Two commonly used non-viral vectors (Lipofectamine 2000 and jet polyethylenimine) were used for the delivery of gene fluorescent protein plasmid in HeLa cancer cells...
June 22, 2018: Journal of Drug Targeting
Ling Qian, Shulin Yu, Zhen Chen, Zhiqiang Meng, Shenglin Huang, Peng Wang
Circular RNA (circRNA), a recently discovered subclass of non-coding RNAs (ncRNAs), forms a covalently closed loop with neither a 5' cap structure nor a 3' polyadenylated tail. Generated from precursor mRNA (pre-mRNA) through "backsplicing" (a type of alternative RNA splicing), the majority of circRNAs are located in the cytoplasm and are widespread among living organisms. They are stable and conserved and exhibit spatiotemporal-specific expression. circRNAs are known to be involved in the development and progression of multiple diseases, including cancer, by acting as microRNA (miRNA) sponges and by regulating processes such as transcription and translation...
June 18, 2018: Biochimica et Biophysica Acta
Lu Zhang, Cuihua Feng, Yamin Zhou, Qiong Zhou
The present study aimed to identify bladder cancer-associated microRNAs (miRNAs) and target genes, and further analyze the potential molecular mechanisms involved in bladder cancer. The mRNA and miRNA expression profiling dataset GSE40355 was downloaded from the Gene Expression Omnibus database. The Limma package in R was used to identify differential expression levels. The Human microRNA Disease Database was used to identify bladder cancer-associated miRNAs and Target prediction programs were used to screen for miRNA target genes...
June 2018: Oncology Letters
Antti P Aalto, Ian A Nicastro, James P Broughton, Laura B Chipman, William P Schreiner, Jerry S Chen, Amy E Pasquinelli
Argonaute (AGO) proteins partner with microRNAs (miRNAs) to target specific genes for post-transcriptional regulation. During larval development in Caenorhabditis elegans, Argonaute-Like Gene 1 (ALG-1) is the primary mediator of the miRNA pathway, while the related ALG-2 protein is largely dispensable. Here we show that in adult C. elegans these AGOs are differentially expressed and, surprisingly, work in opposition to each other; alg-1 promotes longevity, whereas alg-2 restricts lifespan. Transcriptional profiling of adult animals revealed that distinct miRNAs and largely non-overlapping sets of protein-coding genes are misregulated in alg-1 and alg-2 mutants...
June 2018: PLoS Genetics
Tania Islam, Rezanur Rahman, Esra Gov, Beste Turanli, Gizem Gulfidan, Anwarul Haque, Kazım Yalçın Arga, Nurul Haque Mollah
The head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world, but robust biomarkers and diagnostics are still not available. This study provides in-depth insights from systems biology analyses to identify molecular biomarker signatures to inform systematic drug targeting in HNSCC. Gene expression profiles from tumors and normal tissues of 22 patients with histological confirmation of nonmetastatic HNSCC were subjected to integrative analyses with genome-scale biomolecular networks (i...
June 2018: Omics: a Journal of Integrative Biology
Hai-Ping Zhao, Ping Liu, Chang-Min Xu, Guang-Wen Li, Li Gao, Yu-Min Luo
OBJECTIVE: To identify the differentially expressed microRNAs (miRNAs) profiles of yang and yin syndromes in patients with acute ischemic stroke, and to provide the molecular basis of the classififi cation of these two syndrome types in acute ischemic stroke patients. METHODS: A microarray assay was performed to assess the expression pattern of miRNAs in the lymphocyte of acute ischemic stroke patients. Target genes for the deregulated miRNAs were predicated using the online bioinformatic algorithms and functional annotation via Kyoto encyclopedia of genes and genomes pathway analysis for miRNAs predicted targets was carried out...
June 20, 2018: Chinese Journal of Integrative Medicine
Zhen Peng, Shoupu He, Wenfang Gong, Feifei Xu, Zhaoe Pan, Yinhua Jia, Xiaoli Geng, Xiongming Du
BACKGROUND: Salinity is a major abiotic stress that limits upland cotton growth and reduces fibre production worldwide. To reveal genetic regulation via transcript and protein levels after salt stress, we comprehensively analysed the global changes in mRNA, miRNA, and protein profiles in response to salt stress in two contrasting salt-tolerant cotton genotypes. RESULTS: In the current study, proteomic and mRNA-seq data were combined to reveal that some genes are differentially expressed at both the proteomic and mRNA levels...
June 20, 2018: BMC Plant Biology
Ni Wu, Jun Yan, Tao Han, Jijun Zou, Weimin Shen
BACKGROUND: Orofacial clefts include cleft lip only (CLO), cleft palate only (CPO), and cleft lip with palate (CLP). Previously, we reported the expression profile of plasma microRNAs in CLO, CPO, and CLP, respectively. However, the interaction of each subtype remains poorly investigated. METHODS: In this study, we integrated the expression profiles of plasma miRNAs in these 3 subtypes, and assessed the distinct and overlapping dysregulated miRNAs using Venn diagrams...
June 2018: Medicine (Baltimore)
Jongmin Sim, Yeseul Kim, Hyunsung Kim, Su-Jin Shin, Dong-Hoon Kim, Seung Sam Paik, Kiseok Jang
Lung cancer is the most common cause of cancer-associated death worldwide. Postoperative relapse and subsequent metastasis result in a high mortality rate, even in early stage lung cancer. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level and are frequently dysregulated in various cancers. The aim of this study was to identify recurrence-associated miRNAs in early stage lung cancer. To screen for differentially expressed miRNAs related to postoperative recurrence, miRNA microarray data derived from stage I lung adenocarcinoma formalin-fixed paraffin-embedded (FFPE) tissue samples (n = 6) and publically available the Cancer Genome Atlas (TCGA) data were analyzed...
June 2018: Medicine (Baltimore)
Tao Jiang, Junjie Guo, Zhongchun Hu, Ming Zhao, Zhenggang Gu, Shu Miao
BACKGROUND Long noncoding RNAs (lncRNAs) have been revealed to function as competing endogenous RNAs (ceRNAs), which can seclude the common microRNAs (miRNAs) and hence prevent the miRNAs from binding to their ancestral gene. Nonetheless, the role of lncRNA-mediated ceRNAs in prostate cancer has not yet been elucidated. MATERIAL AND METHODS Using The Cancer Genome Atlas (TCGA) database, lncRNA, miRNA, and mRNA profiles from 499 prostate cancer tissues and 52 normal prostate tissues were analyzed with the R package "DESeq" to identify the differentially expressed RNAs...
June 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Leila Jamali, Roghayeh Tofigh, Sara Tutunchi, Ghodratollah Panahi, Fatemeh Borhani, Saeedeh Akhavan, Parisa Nourmohammadi, Sayyed M H Ghaderian, Milad Rasouli, Hamed Mirzaei
Gastric and esophageal cancers are as main cancers of the gastrointestinal (GI) tract, which are associated with poor diagnosis and survival. Several efforts were made in the past few decades to finding effective therapeutic approaches, but these approaches had several problems. Finding new biomarkers is a critical step in finding new approaches for the treatment of these cancers. Finding new biomarkers that cover various aspects of the diseases could provide a choice of suitable therapies and better monitoring of patients with these cancers...
June 19, 2018: Journal of Cellular Physiology
Duo Yu, Yunfeng Li, Zhihui Ming, Hongyong Wang, Zhuo Dong, Ling Qiu, Tiejun Wang
Background: Cervical cancer is one of the most common cancers in women worldwide. Malignant tumors develop resistance mechanisms and are less sensitive to or do not respond to irradiation. With the development of high-throughput sequencing technologies, circular RNA (circRNA) has been identified in an increasing number of diseases, especially cancers. It has been reported that circRNA can compete with microRNAs (miRNAs) to change the stability or translation of target RNAs, thus regulating gene expression at the transcriptional level...
2018: PeerJ
Xiaodong Li, Pujun Gao, Yang Wang, Xiaocong Wang
microRNAs (miRNAs) have been reported to be aberrantly expressed in patients with pancreatic cancer. In present review we explored the biological roles of miRNAs in pancreatic cancer and their clinical value in diagnosis. In the systematic review, the potential value of miRNAs as biomarkers was investigated by reviewing the altered miRNA profiles reported in pancreatic cancer patients in 356 included studies. In the subsequent meta-analysis, we included 17 studies in early diagnosis of pancreatic cancer with a panel of altered miRNAs...
2018: Frontiers in Physiology
Feng Shao, Mei Huang, Futao Meng, Qiang Huang
Gemcitabine resistance is currently the main problem of chemotherapy for advanced pancreatic cancer patients. The resistance is thought to be caused by altered drug metabolism or reduced apoptosis of cancer cells. However, the underlying mechanism of Gemcitabine resistance in pancreatic cancer remains unclear. In this study, we established Gemcitabine resistant PANC-1 (PANC-1-GR) cell lines and compared the circular RNAs (circRNAs) profiles between PANC-1 cells and PANC-1-GR cells by RNA sequencing. Differentially expressed circRNAs were demonstrated using scatter plot and cluster heatmap analysis...
2018: Frontiers in Pharmacology
Na Li, Xiaohan Zhan, Xianquan Zhan
BACKGROUND: Malignant tumors are heterogeneous diseases characterized by different metabolic phenotypes. These were revealed by Warburg effect and reverse Warburg effect phenotypes. However, the molecular mechanism remains largely unknown. METHODS: Isobaric tag for relative and absolute quantification (iTRAQ) proteomics was used to identify mitochondrial differentially expressed proteins (DEPs) of ovarian cancers relative to controls, followed by bioinformatic analysis...
June 16, 2018: Gynecologic Oncology
Sarah Emmett, David C Whiteman, Benedict J Panizza, Annika Antonsson
Squamous cell carcinoma of mucosal sites in the head and neck (HNSCC) is the sixth most common cause of cancer worldwide, and despite advances in conventional management, it still has significant morbidity and mortality associated with both diagnosis and treatment. Advances in our understanding of the biological mechanisms underlying this disease have demonstrated a significant difference between human papillomavirus (HPV)-associated, HPV and tobacco associated, and HPV-negative disease. It remains important to further elucidate the biologic and genetic differences between HPV-associated and tobacco-associated disease, with the aim of earlier diagnosis through screening, and advances in management including the development of novel therapeutic agents...
June 19, 2018: Oncology
Hong-Xiang Wang, Qi-Lin Huang, Jun-Yan Shen, Tao Xu, Fan Hong, Zhen-Yu Gong, Feng Li, Yong Yan, Ju-Xiang Chen
OBJECTIVE: The aim of the present study is to investigate the expression profiles of circular RNAs (circRNAs) in IDH-wild type (IDH-wt) glioblastoma and explore the differences in circRNAs expression between IDH-wt glioblastoma and adjacent normal brain. PATIENTS AND METHODS: circRNA expression profiles were detected by circRNA microarray in three matched pairs of IDH-wt glioblastoma and adjacent normal brain. qRT-PCR was used to verify the differential expression of circRNAs from microarray analysis...
June 12, 2018: Clinical Neurology and Neurosurgery
Ivonne Koeck, Ali Hashemi Gheinani, Ulrich Baumgartner, Erik Vassella, Rémy Bruggmann, Fiona C Burkhard, Katia Monastyrskaya
Bladder outlet obstruction (BOO) and the ensuing clinical lower urinary tract dysfunction are common in elderly patients. BOO is accompanied by urodynamic changes in bladder function and leads to organ fibrosis and ultimately loss of contractility. Comprehensive transcriptome analysis of bladder samples from human patients with different urodynamically-defined phenotypes of BOO revealed tumor necrosis factor TNF-α as the top upstream signaling pathway regulator. Here we validated next-generation sequencing and pathway analysis in cell-based models using bladder smooth muscle and urothelial cells exposed to tumor necrosis factor (TNF)-α...
June 16, 2018: American Journal of Pathology
Zhihua Chen, Mengzhen Zhang, Yuhuan Qiao, Junjuan Yang, Qinan Yin
BACKGROUND: The human cervical carcinoma oncogenic mechanisms still remain elusive. Thus, we proposed to understand the biological role of a newly discovered therapeutic miRNA. METHODS: MiR-1297 related to human cervical carcinoma was selected for this study. TaqMan qRT- PCR assay was used to profile miRNA, phosphatase and tensin homolog (PTEN) expression in randomly chosen tumour with non-tumour tissues, and the apoptosis factors expression. Cell proliferation was monitored by CCK-8 assay and colony formation assay...
June 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
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