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miRNA expression profiling

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https://www.readbyqxmd.com/read/29456662/microrna-profiles-of-bmscs-induced-into-osteoblasts-with-osteoinductive-medium
#1
Zhixiong Yan, Yong Guo, Yang Wang, Yanan Li, Jiahui Wang
MicroRNA (miRNA) plays an important role in cell differentiation and functions as a regulator. Therefore, miRNA is important in the process of bone marrow mesenchymal stem cells (BMSCs) being induced into osteoblasts. In this study, mouse BMSCs were induced with osteoinductive medium, the indices related to osteoblastic differentiation were assayed, including alkaline phosphatase, the deposit of calcium and protein levels of osteocalcin. Using miRNA microarray and reverse transcription-quantitative polymerase chain reaction analyses, differentially expressed miRNAs in the cells, which were induced with osteoinductive medium, were selected and identified...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29455432/serum-mirna-27a-and-mirna-18b-as-potential-predictive-biomarkers-of-hepatitis-c-virus-associated-hepatocellular-carcinoma
#2
Nearmeen M Rashad, Amal S El-Shal, Sally M Shalaby, Salem Y Mohamed
Hepatitis C virus (HCV) infection remains the main risk factor for chronic hepatitis (CHC), liver cirrhosis, and hepatocellular carcinoma (HCC). Changes in microRNA (miRNA) profiles can be associated with HCV infection and may either favor or inhibit the virus and/or its complication. Moreover, miRNAs have emerged as key regulators of various cancers including HCC. The aim of this work was to investigate the potentail role of miRNA-27a and miRNA-18b expression levels as non-invasive predictive biomarkers of hepatitis C virus-associated HCC...
February 17, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29453091/identification-of-mirnas-and-their-targets-in-maize-in-response-to-sugarcane-mosaic-virus-infection
#3
Zihao Xia, Zhenxing Zhao, Mingjun Li, Ling Chen, Zhiyuan Jiao, Yuanhua Wu, Tao Zhou, Weichang Yu, Zaifeng Fan
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses. Maize (Zea mays L.) is a global economically important food and forage crop. To date, a number of maize miRNAs have been identified as being involved in plant development and stress responses. However, the miRNA-mediated gene regulatory networks responsive to virus infections in maize remain largely unknown. In this study, the profiles of small RNAs in buffer- and Sugarcane mosaic virus (SCMV)-inoculated maize plants were obtained by high-throughput sequencing, respectively...
January 31, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29451334/microrna-profiling-between-bacillus-thuringiensis-cry1ab-susceptible-and-resistant-european-corn-borer-ostrinia-nubilalis-h%C3%A3-bner
#4
T Yu, X Li, B-S Coates, Q Zhang, B-D Siegfried, X Zhou
Transgenic maize hybrids that express insecticidal Bacillus thuringiensis (Bt) crystalline (Cry) protein toxins effectively protect against the European corn borer, Ostrinia nubilalis, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels of O. nubilalis resistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post-transcriptional gene regulation. Their potential roles in the evolution of Bt resistance, however, remain largely unknown...
February 16, 2018: Insect Molecular Biology
https://www.readbyqxmd.com/read/29451210/intravenous-morphine-self-administration-alters-accumbal-microrna-profiles-in-the-mouse-brain
#5
Juhwan Kim, Heh-In Im, Changjong Moon
A significant amount of evidence indicates that microRNAs (miRNAs) play an important role in drug addiction. The nucleus accumbens (NAc) is a critical part of the brain's reward circuit and is involved in a variety of psychiatric disorders, including depression, anxiety, and drug addiction. However, few studies have examined the expression of miRNAs and their functional roles in the NAc under conditions of morphine addiction. In this study, mice were intravenously infused with morphine (0.01, 0.03, 0.3, 1 and 3 mg/kg/infusion) and showed inverted U-shaped response...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29448111/in-silico-screening-of-proteins-targeting-circulating-mirnas-for-improved-diagnosis-of-multiple-myeloma
#6
Shradha Suyal, Manish Pratap Singh, Himanshu Shekhar, Sameer Srivastava
Multiple Myeloma (MM) is a B-cell malignancy, which is characterized by the expansion of clonal plasma cells in the bone marrow, leading to abnormal accumulation of monoclonal antibodies in circulation. Certain circulating miRNAs are deregulated in MM and their differential expression profiles in body fluids can be quantified and used discriminate between the premalignant and malignant stages of MM. Our study identifies protein which would show affinity for a selected panel of circulating miRNAs deregulated in MM...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29447975/profiling-of-novel-micrornas-elicited-by-ev71-and-ca16-infection-in-human-bronchial-epithelial-cells-using-high-throughput-sequencing
#7
Jie Song, Yajie Hu, Xi Jiang, Wenbing Zhu, Zhongxiang Wu, Shaozhong Dong
Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are two major etiologic agents associated with hand, foot, and mouth disease (HFMD) worldwide. Despite that they both belong to the Enterovirus genus of the Picornaviridae family, there are many differences in the infection process of these viruses. However, the underlying mechanisms have not been elucidated. Multiple studies indicated that microRNAs (miRNAs) can play critical roles in the host-pathogen interaction. Our previous study reported that EV71 and CA16 infection leads to differential expression of miRNAs in human bronchial epithelial (16HBE) cells...
February 12, 2018: Virus Research
https://www.readbyqxmd.com/read/29447339/integrated-microrna-and-mrna-network-analysis-of-the-human-myometrial-transcriptome-in-the-transition-from-quiescence-to-labor
#8
William E Ackerman, Irina A Buhimschi, Douglas Brubaker, Sean Maxwell, Kara M Rood, Mark R Chance, Hongwu Jing, Sam Mesiano, Catalin S Buhimschi
We conducted integrated transcriptomics network analyses of miRNA and mRNA interactions in human myometrium to identify novel molecular candidates potentially involved in human parturition. Myometrial biopsies were collected from women undergoing primary Cesarean deliveries in well-characterized clinical scenarios: 1) spontaneous term labor (TL, n = 5); 2) term non-labor (TNL, n = 5); 3) spontaneous preterm birth (PTB) with histologic chorioamnionitis (PTB-HCA, n = 5); and 4) indicated PTB non-labor (PTB-NL, n = 5)...
February 13, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29447268/circulating-small-non-coding-rnas-reflect-ifn-status-and-b-cell-hyperactivity-in-patients-with-primary-sj%C3%A3-gren-s-syndrome
#9
Ana P Lopes, Maarten R Hillen, Eleni Chouri, Sofie L M Blokland, Cornelis P J Bekker, Aike A Kruize, Marzia Rossato, Joel A G van Roon, Timothy R D J Radstake
BACKGROUND: Considering the important role of miRNAs in the regulation of post-transcriptional expression of target genes, we investigated circulating small non-coding RNAs (snc)RNA levels in patients with primary Sjögren's syndrome (pSS). In addition we assessed if serum sncRNA levels can be used to differentiate patients with specific disease features. METHODS: Serum RNA was isolated from 37 pSS patients as well as 21 patients with incomplete Sjögren's Syndrome (iSS) and 17 healthy controls (HC) allocated to two independent cohorts: discovery and validation...
2018: PloS One
https://www.readbyqxmd.com/read/29445089/mir-27a-5p-increases-steer-fat-deposition-partly-by-targeting-calcium-sensing-receptor-casr
#10
Wucai Yang, Keqiong Tang, Yaning Wang, Linsen Zan
Castration increases fat deposition, improving beef quality in cattle. Here, the steer group exhibited a significantly higher intramuscular fat (IMF) content than the bull group. To determine the potential roles of microRNAs (miRNAs) in castration-induced fat deposition, differential expression patterns of miRNA in liver tissue were investigated in bulls and steers. A total of 7,827,294 clean reads were obtained from the bull liver library, and 8,312,483 were obtained from the steer liver library; 452 conserved bovine miRNAs and 20 novel miRNAs were identified...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29444641/oasis-2-improved-online-analysis-of-small-rna-seq-data
#11
Raza-Ur Rahman, Abhivyakti Gautam, Jörn Bethune, Abdul Sattar, Maksims Fiosins, Daniel Sumner Magruder, Vincenzo Capece, Orr Shomroni, Stefan Bonn
BACKGROUND: Small RNA molecules play important roles in many biological processes and their dysregulation or dysfunction can cause disease. The current method of choice for genome-wide sRNA expression profiling is deep sequencing. RESULTS: Here we present Oasis 2, which is a new main release of the Oasis web application for the detection, differential expression, and classification of small RNAs in deep sequencing data. Compared to its predecessor Oasis, Oasis 2 features a novel and speed-optimized sRNA detection module that supports the identification of small RNAs in any organism with higher accuracy...
February 14, 2018: BMC Bioinformatics
https://www.readbyqxmd.com/read/29444515/comprehensive-mirna-analysis-of-human-umbilical-cord-derived-mesenchymal-stromal-cells-and-extracellular-vesicles
#12
Xiang-Yu Zou, Yongjiang Yu, Sihao Lin, Liang Zhong, Jie Sun, Guangyuan Zhang, Yingjian Zhu
BACKGROUND/AIMS: Mesenchymal stromal cells (MSCs) participate in the tissue-specific repair of many different organs, especially the kidney. Their effects are primarily mediated by the paracrine release of factors including extracellular vesicles (EVs), which are composed of micro-vesicles and exosomes. The corresponding microRNAs (miRNAs) of EVs are considered important for their biological functions. METHODS: MSCs were cultured from the human umbilical cord, and EVs were isolated from the medium...
February 8, 2018: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29442265/integrated-mrna-and-mirna-profiling-revealed-deregulation-of-cellular-stress-response-in-bone-marrow-mesenchymal-stem-cells-derived-from-patients-with-immune-thrombocytopenia
#13
Jia-Min Zhang, Xiao-Lu Zhu, Jing Xue, Xiao Liu, X Long Zheng, Ying-Jun Chang, Kai-Yan Liu, Xiao-Jun Huang, Xiao-Hui Zhang
Our understanding of the pathogenesis of immune thrombocytopenia (ITP) remains limited due to the complexity and heterogeneity of the disease. Recently, we observed that bone marrow mesenchymal stem cells (MSCs) derived from ITP patients exhibited growth defects and functional abnormalities that might be involved in the breakdown of self-tolerance. However, the underlying mechanism remains unclear. In this study, we profiled the expression of both mRNAs and miRNAs by utilizing the microarray technique and deciphered the mechanism underlying the impairment of MSCs derived from ITP patients (MSC-ITP)...
February 13, 2018: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/29440731/microarray-profile-of-circular-rnas-identifies-hsa_circ_0014130-as-a-new-circular-rna-biomarker-in-non-small-cell-lung-cancer
#14
Shaoyan Zhang, Xiaoli Zeng, Ting Ding, Lin Guo, Yulong Li, Songlei Ou, Hui Yuan
Accumulating evidence has revealed that aberrant Circular RNAs (circRNAs) expression plays important roles in carcinogenesis and tumor progression. However, their role in non-small cell lung cancer (NSCLC) remains unclear. In this study, we first used circRNA microarrays to screen for tumour-specific circRNA candidates in between NSCLC (n = 3) and adjacent lung (n = 3) tissue. Among the circRNA expression profile, two circRNAs (hsa_circ_0014130 and hsa_circ_0016760) were selected for validation in ten pairs of NSCLC and adjacent non-cancerous tissues by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR)...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440068/molecular-characterization-of-renal-cell-carcinoma-a-potential-three-microrna-prognostic-signature
#15
Soum D Lokeshwar, Asif Talukder, Travis J Yates, Martin J P Hennig, Michael Garcia-Roig, Sarrah S Lahorewala, Naureen N Mullani, Zachary Klaassen, Bruce R Kava, Murugesan Manoharan, Mark S Soloway, Vinata Lokeshwar
BACKGROUND: Aberrantly expressed microRNAs (miR) promote renal cell carcinoma (RCC) growth and metastasis and are potentially useful biomarkers for metastatic disease. However, a consensus clinically significant miRNA signature has not been identified. To identify a miRNA signature for predicting clinical outcome in RCC patients we used a four-pronged interconnected approach. METHODS: Differentially expressed miRs were identified and analyzed in 113 specimens (Normal kidney: 59; tumor: 54)...
February 12, 2018: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/29439878/thickness-and-an-altered-mirna-expression-in-the-epicardial-adipose-tissue-is-associated-with-coronary-heart-disease-in-sudden-death-victims
#16
Josep Marí-Alexandre, Moises Barceló-Molina, Jorge Sanz-Sánchez, Pilar Molina, Jennifer Sancho, Yolanda Abellán, María Luisa Santaolaria-Ayora, Juan Giner, Luis Martínez-Dolz, Amparo Estelles, Aitana Braza-Boïls, Esther Zorio
INTRODUCTION AND OBJECTIVES: An increased epicardial adipose tissue (EAT) thickness has become a new risk factor for coronary heart disease (CHD). We aimed to study the role of EAT dysfunction as a CHD marker by focusing on its thickness and microRNA (miRNA) expression profile, and the potential factors possibly influencing them. METHODS: One hundred and fifty-five CHD sudden cardiac death victims and 84 non-CHD-sudden death controls were prospectively enrolled at autopsy...
February 10, 2018: Revista Española de Cardiología
https://www.readbyqxmd.com/read/29437039/microrna-1246-is-an-exosomal-biomarker-for-aggressive-prostate-cancer
#17
Divya Bhagirath, Thao Ly Yang, Nathan Bucay, Kirandeep Sekhon, Shahana Majid, Varahram Shahryari, Rajvir Dahiya, Yuichiro Tanaka, Sharanjot Saini
Due to high heterogeneity, molecular characterization of prostate cancer (PCa) based on biopsy sampling is often challenging. Hence, a minimally invasive method to determine the molecular imprints of a patient's tumor for risk stratification would be advantageous. In this study, we employ a novel, digital amplification-free quantification method using the nCounter technology (Nanostring Technologies) to profile exosomal serum miRNAs (ex-miRNA) from aggressive PCa cases, benign prostatic hyperplasia (BPH), and disease-free controls...
February 1, 2018: Cancer Research
https://www.readbyqxmd.com/read/29436608/microrna-expression-profiles-of-scar-and-normal-tissue-from-patients-with-posterior-urethral-stricture-caused-by-pelvic-fracture-urethral-distraction-defects
#18
Kaile Zhang, Jun Chen, Dongliang Zhang, Lin Wang, Weixin Zhao, David Yun-Te Lin, Rong Chen, Hong Xie, Xiaoyong Hu, Xiaolan Fang, Qiang Fu
Pelvic fracture urethral distraction defect (PFUDD) seriously affects the quality of life of patients. At present, there are few effective drug treatments available for PFUDD‑induced urethral stricture, which is associated with fibrosis and scar formation in urethra lumen. Emerging evidence suggests that microRNAs (miRNAs/miRs) may be involved in the regulation of fibrosis, and analysis of miRNA expression profiles in urethral scar and normal urethra tissues may therefore benefit the discovery of novel treatments for urethral stricture with micro invasive procedures...
February 12, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29435933/comprehensive-measurement-of-gene-silencing-involving-endogenous-micrornas-in-mammalian-cells
#19
Masashi Fukuoka, Hirohiko Hohjoh
MicroRNAs (miRNAs) are functional small noncoding RNAs that work as mediators in gene silencing and that play important roles in gene regulation. A number of miRNAs have been found and their expression profiles have been examined by means of various microarray systems and real-time polymerase chain reaction (PCR) systems. Conventional microarrays as well as real-time PCR are able to detect existing miRNAs, in which inactive miRNAs that hardly contribute to gene silencing may be also contained. Here, we describe a comprehensive miRNA bioassay system with reporter genes for the detection of active miRNAs that are present in the RNA-induced silencing complexes, and actually working as mediators in gene silencing...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435930/quantum-language-of-microrna-application-for-new-cancer-therapeutic-targets
#20
Yoichi Robertus Fujii
MicroRNA (miRNA) is the noncoding gene: therefore, the miRNA gene inheritably controls protein gene expression through transcriptional and post-transcriptional levels. Aberrant expression of miRNA genes causes various human diseases, especially cancers. Although cancer is a complex disease, cancer/miRNA implication has yet been grasped from the perspective of miRNA profile in bed side. Since miRNA is the mobile genetic element, the clinical verification of miRNA in microvesicle of blood is too much straggle to predict potential cancer/miRNA associations without bioinformatical computing...
2018: Methods in Molecular Biology
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