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Endometrial scratching

Vijay Kumar Sirohi, Pooja Popli, Pushplata Sankhwar, Jyoti Bala Kaushal, Kanchan Gupta, Murli Manohar, Anila Dwivedi
Although curcumin shows anti-proliferative and anti-inflammatory activities in various cancers, the effect of curcumin on cellular migration in endometrial adenocarcinoma cells remains to be understood. The current investigation was aimed to explore the anti-proliferative and anti-migratory effects of curcumin and its mechanism of action in endometrial cancer cells. Our in-vitro and in-vivo experimental studies showed that curcumin inhibited the proliferation of endometrial cancer cells and suppressed the tumor growth in Ishikawa xenograft mouse model...
March 16, 2017: Journal of Nutritional Biochemistry
Jacqueline A Maybin, Lyndsey Boswell, Vicky J Young, William C Duncan, Hilary O D Critchley
Context: Heavy menstrual bleeding (HMB) is common and incapacitating. Aberrant menstrual endometrial repair may result in HMB. The transforming growth factor (TGF)-β superfamily contributes to tissue repair, but its role in HMB is unknown. Objective: We hypothesized that TGF-β1 is important for endometrial repair, and women with HMB have aberrant TGF-β1 activity at menses. Participants/Setting: Endometrial biopsies were collected from women, and menstrual blood loss objectively measured [HMB >80 mL/cycle; normal menstrual bleeding (NMB) <80 mL]...
April 1, 2017: Journal of Clinical Endocrinology and Metabolism
Saghar Salehpour, Marzieh Zamaniyan, Nasrin Saharkhiz, Shahrzad Zadeh Modares, Sedighe Hosieni, Samira Seif, Narges Malih, Parinaz Rezapoor, Mohammad-Reza Sohrabi
BACKGROUND: Recurrent implantation failure is one of the most issues in IVF cycles. Some researchers found that beneficial effects of endometrial Scratching in women with recurrent implantation failure, while some authors demonstrated contrary results. OBJECTIVE: The present study aimed to investigate the effect of intrauterine. Saline infusion as a form of endometrial injury, during fresh in vitro fertilization-embryo transfer cycle, among patients with recurrent implantation failure...
September 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
Z Z Lu, L Yan, H Zhang, M J Li, X H Zhang, X X Zhao
OBJECTIVE: To investigate the effect and mechanism of mifepristone on the migration of human endometrial carcinoma cells. METHODS: A human endometrial carcinoma cell line, Ishikawa cells, was cultured in vitro and treated with mifepristone at different concentrations. Wound healing assay was applied to detect the migration of Ishikawa cells. RT-PCR and methylation-specific PCR (MSP) were used to detect the levels of H19 mRNA and its DNA methylation. Western-blot was used to detect the expressions of HMGA2 and epithelial to mesenchymal transition (EMT) related proteins...
June 23, 2016: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
Mehmet Murat Seval, Yavuz Emre Şükür, Batuhan Özmen, Özgür Kan, Murat Sönmezer, Bülent Berker, Cem Atabekoğlu
OBJECTIVE: To investigate the effect of additional endometrial scratching procedure during hysteroscopy on assisted reproductive technology (ART) cycle outcomes in repeated implantation failure (RIF) patients without endometrial or uterine abnormalities on hysteroscopic evaluation. MATERIALS AND METHODS: Three hundred and forty-five RIF patients who underwent ART at a university-based infertility clinic between January 2011 and June 2015 were recruited in this retrospective cohort study...
December 2016: Gynecological Endocrinology
Xavier Santamaria, Nora Katzorke, Carlos Simón
PURPOSE OF REVIEW: Since its first description in 2003, the endometrial scratching procedure has been the topic of over 1000 studies. This procedure, used to improve endometrial receptivity for assisted reproduction, is accessible - any gynecologist can easily perform it - and has been adapted into clinical routine by some reproductive units. However, the available data are controversial, and no biological plausibility exists to support the use of this intervention. This study aims to critically review the existing data, focusing on the last 2 years, regarding the efficiency of endometrial scratching...
August 2016: Current Opinion in Obstetrics & Gynecology
Shifan Ren, Yefang Zhou, Xiaoling Fang, Xiaoling She, Yilin Wu, Xianqing Wu
OBJECTIVE: To investigate the role of phosphatase of regenerating liver-3 (PRL-3) in the 17β-estradiol (E2)- and interleukin 6 (IL-6)-induced migration of endometrial stromal cells (ESCs) from ectopic endometrium. METHODS: Ectopic endometrial tissues were collected from patients with endometriosis, and PRL-3 expression in ectopic and eutopic endometrium was examined by immunohistochemistry. Endometrial stromal cells isolated from ectopic endometrium were treated with E2, progesterone (P), IL-6, or sodium orthovanadate (Sov) to inhibit PRL-3...
May 24, 2016: Reproductive Sciences
Tsutomu Miyamoto, Hiroyasu Kashima, Yasushi Yamada, Hisanori Kobara, Ryoichi Asaka, Hirofumi Ando, Shotaro Higuchi, Koichi Ida, David Hamisi Mvunta, Tanri Shiozawa
PURPOSE: Lipocalin 2 (LCN2) is a secretory protein that is involved in various physiological processes including iron transport. We previously identified LCN2 as an up-regulated gene in endometrial carcinoma, and found that the overexpression of LCN2 and its receptor, SLC22A17, was associated with a poor prognosis. However, the functions and mechanism of action of LCN2 currently remain unclear. METHODS: The LCN2-overexpressing endometrial carcinoma cell lines, HHUA and RL95-2, and LCN2-low-expressing one, HEC1B, were used...
2016: PloS One
Sarah Lensen, Wellington Martins, Carolina Nastri, Lynn Sadler, Cindy Farquhar
BACKGROUND: The success rate of infertility treatments remains modest. Endometrial injury has been suggested as an intervention to increase the probability of pregnancy in women undergoing assisted reproductive technologies such as in vitro fertilisation (IVF). The majority of studies and systematic reviews have reported that endometrial injury improves the outcomes of IVF, intrauterine insemination and natural conception; however, the size and quality of the studies are poor. The low quality of the available evidence questions the presence of any real beneficial effect, and the applicability of the intervention in different populations remains unclear...
April 27, 2016: Trials
S Lensen, L Sadler, C Farquhar
STUDY QUESTION: What proportion of clinicians across Australia, New Zealand and the UK are currently offering or recommending endometrial scratching for subfertility? SUMMARY ANSWER: Eighty-three percent of clinicians responding to this survey are recommending endometrial scratching to women undergoing IVF. WHAT IS KNOWN ALREADY: Endometrial scratching is currently being proposed as a technique to increase the probability of implantation in women undergoing IVF...
June 2016: Human Reproduction
Jennifer Ka Yee Ko, Ernest Hung Yu Ng
PURPOSE OF REVIEW: To review updated information on the influence of endometrial scratching on IVF. RECENT FINDINGS: Endometrial receptivity remains an important rate-limiting step affecting the success of IVF. The current evidence on the effect of endometrial scratching on IVF ranges from marked improvement, no difference to a potentially negative impact. The heterogeneity of studies presents a challenge in interpretation of data for routine clinical practice. SUMMARY: Endometrial scratching performed in the preceding cycle is associated with improved clinical pregnancy and live birth rates in women with recurrent implantation failure, but not in unselected subfertile women undergoing IVF...
June 2016: Current Opinion in Obstetrics & Gynecology
Arsène Mekinian, Jonathan Cohen, Jaume Alijotas-Reig, Lionel Carbillon, Pascale Nicaise-Roland, Gilles Kayem, Emile Daraï, Olivier Fain, Marie Bornes
To describe and analyze the benefit of immunomodulatory drugs for recurrent miscarriages and implantation failures. The literature research was conducted in Medline, Embase and Cochrane Library concerning recurrent miscarriages and implantation failures and steroids, progesterone, intralipids, TNF-α antagonists, G-CSF, hydroxychloroquine, intravenous immunoglobulins, endometrial scratching. Using meta-analysis, modest benefit was found for progesterone to obtain a live birth, with odds ratio at 1.38 (95% CI: 1...
July 2016: American Journal of Reproductive Immunology: AJRI
Ilia Bord, Belle Tamir, Avraham Harlev, Iris Har-Vardi, Eitan Lunenfeld, Michael Friger, Eliahu Levitas
PURPOSE: To evaluate the characteristics of patients and IVF cycles with recurrent implantation failure who eventually succeeded to conceive compared to those who failed to do so. METHODS: In a retrospective study, we explored our database for patients younger than 35 years old who underwent at least three unsuccessful fresh IVF cycles. The following parameters were analyzed: cause of infertility, FSH level, stimulation cycle characteristics, fertilization rate, the type of luteal support, and cycle outcome...
April 2016: Archives of Gynecology and Obstetrics
Leena Wadhwa, Amrita Pritam, Taru Gupta, Sangeeta Gupta, Sarika Arora, Rajkumar Chandoke
OBJECTIVE: The objective was to evaluate the effect of endometrial biopsy (EB) on intrauterine insemination (IUI) outcome in controlled ovarian stimulation (COS) cycle. DESIGN: Prospective randomized control study. SETTING: Tertiary care center. MATERIALS AND METHODS: A total of 251 subjects were enrolled in the study. Subjects undergoing COS with IUI were randomly allocated into three groups. Group A: EB was taken between D19 and 24 of the spontaneous menstrual cycles that precedes the fertility treatment and IUI, which was done in next cycle (n = 86)...
July 2015: Journal of Human Reproductive Sciences
Yinghua Li, Yunpeng Xie, Dan Cui, Yanni Ma, Linlin Sui, Chenyang Zhu, Hui Kong, Ying Kong
BACKGROUND/AIMS: Osteopontin (OPN) is an Extracellular Matrix (ECM) molecule and is involved in many physiologic and pathologic processes, including cell adhesion, angiogenesis and tumor metastasis. OPN is a well-known multifunctional factor involved in various aspects of cancer progression, including endometrial cancer. In this study, we examined the significance of OPN in endometrial cancer. METHODS: The proliferation, migration and invasion ability of HEC-1A cells were detected by Cell Counting Kit-8 (CCK-8), Wound scratch assay and transwell...
2015: Cellular Physiology and Biochemistry
Lijun Wang, Ying Wang, Huaqing Du, Yao Jiang, Zhichao Tang, Hongyi Liu, Hua Xiang, Hong Xiao
PURPOSES: ER520, a derivative of indenoisoquinoline, is a patented compound. This study was designed to screen its biological properties and to evaluate its antineoplastic and antiangiogenic effect. METHODS: Western blot was employed to monitor the ERα and ERβ protein expression in human breast cancer MCF-7 cells and endometrial carcinoma Ishikawa cells. MTT assay was employed to determine cell proliferation. Cell adhesion, scratch and Transwell assay were utilized to estimate the ability of cellular adhesion, migration and invasion...
December 2015: Cancer Chemotherapy and Pharmacology
Waleed El-Khayat, Mostafa Elsadek, Waleed Saber
OBJECTIVE: To evaluate the role of endometrial injury in the cycle preceding ovarian stimulation for intrauterine insemination (IUI) cycle on the clinical pregnancy rate. STUDY DESIGN: This was a prospective randomized controlled trial which included three hundred and thirty two infertile women with an indication for IUI. The subjects were randomly divided into two groups. The intervention group (group A) (n=166) subjects underwent office hysteroscopy with endometrial injury using grasping forceps with teeth, while the control group (group B) (n=166) subjects underwent office hysteroscopy alone without endometrial injury...
November 2015: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Ahmed M Maged, Hesham Al-Inany, Khaled M Salama, Ibrahim I Souidan, Hesham M Abo Ragab, Noura Elnassery
OBJECTIVE: To explore the impact of endometrial scratch injury (ESI) on intrauterine insemination (IUI) success. METHODS: One hundred and fifty four infertile women received 100 mg of oral clomiphene citrate for 5 days starting on day 3 of the menstrual cycle. Patients were randomized to 2 equal groups: Group C received IUI without ESI and group S had ESI. Successful pregnancy was confirmed by ultrasound. RESULTS: 13, 21, and 10 women got pregnant after the first, second, and third IUI trials, respectively, with 28...
February 2016: Reproductive Sciences
Mei Yang, Chunfan Jiang, Hua Chen, Yan Nian, Zhimiao Bai, Chunfang Ha
BACKGROUND: Endometriosis, which shares certain characteristics with cancers, may cause abnormal expression of proteins involved in cell migration. Endometrial epithelial cells (EECs) are believed to play an important role in endometriotic migration. The aim of this study was to investigate the relationship between the expression of osteopontin (OPN) and matrix metalloproteinase-9 (MMP-9) in endometriotic migration. METHODS: We performed primary culture of EECs and investigated the expression of OPN and MMP-9 in EECs regulated by 17beta-estradiol (E2)...
August 20, 2015: Reproductive Biology and Endocrinology: RB&E
Xiao-Hui Zhang, Zhao-Zhao Liu, Mao-Xing Tang, Yong-Hong Zhang, Lian Hu, Ai-Hua Liao
OBJECTIVE: To establish a mouse model for endometrial injury and determine the underlying mechanism regarding its favorable effect on embryo implantation. STUDY DESIGN: Female Kunming mice were randomly allocated into 4 groups: group I, normal control; group II, injury procedure control; and group III and group IV, the mice being scratched with a blunt syringe on the right uterine horn or both, respectively. All the mice were mated with the males during the next estrus phase...
November 2015: Reproductive Sciences
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