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https://www.readbyqxmd.com/read/29444517/acetyl-coa-synthetase-2-promotes-cell-migration-and-invasion-of-renal-cell-carcinoma-by-upregulating-lysosomal-associated-membrane-protein-1-expression
#1
Lv Yao, Xiaoqiang Guo, Yaoting Gui
BACKGROUND/AIMS: Reprogramming energy metabolism is an emerging hallmark of many cancers, and this alteration is especially evident in renal cell carcinomas (RCCs). However, few studies have been conducted on lipid metabolism. This study investigated the function and mechanism of lipid metabolism-related acetyl-CoA synthetase 2 (ACSS2) in RCC development, cell migration and invasion. METHODS: Quantitative real-time PCR (qRT-PCR) was used to determine the expression of ACSS2 in cancer tissue and adjacent tissue...
February 8, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29443770/whether-innate-immune-together-with-genetic-factor-are-involved-in-leukemic-arthritis-a-case-report-and-review-of-the-literature
#2
Danyi Xu, Guanhua Xu, Jin Lin
We report a case of leukemic arthritis (LA) of monocytic differentiation, which presented with spondyloarthritis-like symptoms and a positive human leukocyte antigen-B27, and discuss its potential mechanisms.The patient was admitted because of pain in her right knee and lower back for 18 months. Magnetic resonance imaging showed diffuse hyperintense signal in the bilateral liac bones and bone marrow edema and synovitis in the right knee.The diagnosis of acute monocytic leukemia and LA were concluded by bone marrow aspiration and flow cytometry of the synovial fluid...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29443732/curcumin-induces-apoptosis-and-inhibits-proliferation-in-infantile-hemangioma-endothelial-cells-via-downregulation-of-mcl-1-and-hif-1%C3%AE
#3
Suhui Lou, Yanfang Wang, Zujiang Yu, Kelei Guan, Quancheng Kan
BACKGROUND: Curcumin has been used as an alternative medicine for the treatment of infantile hemangiomas (IHs); however, the mechanism underlying the effectiveness of curcumin in IHs remains largely unclear. METHODS: In this study, we isolated primary human hemangioma endothelial cells (HemECs) from fresh surgical specimens of 3 patients. We treated HemECs by curcumin and investigated the alterations in proliferative and apoptotic signaling pathways with cell counting kit-8, flow cytometry, western blotting, immunofluorescence, and real-time polymerase chain reaction...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29442453/transcriptomic-analysis-of-the-pi3k-akt-signaling-pathway-reveals-the-dual-role-of-the-c-jun-oncogene-in-cytotoxicity-and-the-development-of-resistance-in-hl-60-leukemia-cells-in-response-to-arsenic-trioxide
#4
Joanna Roszak, Anna Smok-Pieniążek, Maciej Stępnik
BACKGROUND: Arsenic trioxide (ATO) is a well-recognized antileukemic drug used for the treatment of newly diagnosed and relapsed acute promyelocytic leukemia (APL). A major drawback of therapy with ATO is the development of APL cell resistance, the mechanisms of which are still not clear. OBJECTIVES: The aim of this study was to investigate the role of the PI3K/Akt signaling pathway in ATOtreated human acute myeloid leukemia (HL-60) cells and in ATO-resistant clones...
December 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29442376/multiple-system-atrophy-and-apolipoprotein-e
#5
Kotaro Ogaki, Yuka A Martens, Michael G Heckman, Shunsuke Koga, Catherine Labbé, Oswaldo Lorenzo-Betancor, Anna I Wernick, Ronald L Walton, Alexandra I Soto, Emily R Vargas, Henrietta M Nielsen, Shinsuke Fujioka, Takahisa Kanekiyo, Ryan J Uitti, Jay A van Gerpen, William P Cheshire, Zbigniew K Wszolek, Phillip A Low, Wolfgang Singer, Dennis W Dickson, Guojun Bu, Owen A Ross
BACKGROUND: Dysregulation of the specialized lipid metabolism involved in myelin synthesis and maintenance by oligodendrocytes has been associated with the unique neuropathology of MSA. We hypothesized that apolipoprotein E, which is associated with neurodegeneration, may also play a role in the pathogenesis of MSA. OBJECTIVE: This study evaluated genetic associations of Apolipoprotein E alleles with risk of MSA and α-synuclein pathology, and also examined whether apolipoprotein E isoforms differentially affect α-synuclein uptake in a oligodendrocyte cell...
February 14, 2018: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29442262/nutrients-antioxidant-capacity-and-safety-of-hot-water-extract-from-sugar-maple-acer-saccharum-m-and-red-maple-acer-rubrum-l-bark
#6
Sagar Bhatta, Cristina Ratti, Patrice E Poubelle, Tatjana Stevanovic
Sugar maple (Acer saccharum M.) and red maple (Acer rubrum L.) barks were treated with hot water to extract nutrients in order to explore, for the first time, its potential as safe dietary antioxidants. The organic and inorganic nutrients of these extracts, as well as their safety on human PLB-985 cells differentiated into neutrophils-like cells, were determined. Proximate analysis showed that both bark extracts were low in moisture and fat. Sugar maple bark extract (SM-BX) showed crude protein and ash content higher than those found in red maple bark extract (RM-BX)...
February 13, 2018: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/29442010/ganoderic-acid-a-potentiates-the-antioxidant-effect-and-protection-of-mitochondrial-membranes-and-reduces-the-apoptosis-rate-in-primary-hippocampal-neurons-in-magnesium-free-medium
#7
Z M Jiang, H B Qiu, S Q Wang, J Guo, Z W Yang, S B Zhou
Ganoderma lucidum extracts have shown antiepileptic effects in in vivo and in vitro studies. In this work, primary hippocampal neurons cultured in magnesium-free medium were used to study the neuroprotective effects of ganoderic acid A and B (GA-A and GA-B) on superoxide dismutase (SOD) activity and mitochondrial membrane potential, to improve our understanding of their antiepileptic effect. The activity of SOD was determined by the xanthine oxidase assay, the variations of mitochondrial membrane potential and cell apoptosis were measured by JC-1 fluorescent staining and flow cytometry...
February 1, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29441982/microrna-17-3p-promotes-keratinocyte-cells-growth-and-metastasis-via-targeting-myot-and-regulating-notch1-nf-%C3%AE%C2%BAb-pathways
#8
Hongshan Yan, Kunxiu Song, Guohui Zhang
Wound healing is a fundamental biological process to restore skin integrity. The role of microRNAs (miRNAs) during this process remains elusive. Thus, our study aimed to investigate the biological functions and its molecular mechanisms of miR-17-3p in cutaneous wound healing. Human keratinocyte cell line HaCaT was transfected with miR-17-3p mimic, antisense oligonucleotides (ASO)-miR-17-3p and corresponding controls respectively. After transfection, MTT, flow cytometry, qRT-PCR and western blot were performed to analyze cell viability, colony-formation and cell cycle...
September 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441938/ubr5-promotes-cell-proliferation-and-inhibits-apoptosis-in-colon-cancer-by-destablizing-p21
#9
S Q Ji, Y X Zhang, B H Yang
UBR5 is recently recognized as a key player in a large number of prevalent cancers. In this study, we sought to explore the connection of UBR5 expression with cell proliferation, apoptosis, as well as the regulation mechanism in colon cancer cell line. SiUBR5 or oeUBR5 were separately applied to interfere the expression of UBR5. Western blot, DNA gel electrophoresis and qPCR were performed to detect the expression of UBR5 at mRNA and protein level. Then MTT and flow cytometry were used to explore the proliferation and apoptosis in a colon cancer cell line in vitro...
July 3, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441908/microrna-340-induces-apoptosis-by-downregulation-of-bag3-in-ovarian-cancer-skov3-cells
#10
Fei Qu, Xiufen Wang
Aberrant expression of miR-340 has been found in several kinds of cancers including ovarian cancer. Pro-apoptotic and anti-metastasis roles of miR-340 in ovarian cancer have also been reported; however, the underling molecular mechanisms by which miR-340 suppresses ovarian cancer are still unclear. This study focused on the role and molecular mechanism of miR-340 in ovarian cancer. Human ovarian carcinoma SKOV3 cells were used and transfected with miR-340 mimic, miR-340 inhibitor and their correspondingly negative controls (mimic control and inhibitor control)...
August 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441907/microrna-335-inhibits-colorectal-cancer-hct116-cells-growth-and-epithelial-mesenchymal-transition-emt-process-by-targeting-twist1
#11
Jingyuan Wang, Xuemei Wang, Fengjun Liu, Yuguang Fu
Colorectal cancer is one of the most commonly diagnosed cancers. Recently, several microRNAs (miRNAs) have been characterized as oncogenes or tumor suppressors in colorectal cancer. This study was aimed to explore the tumor suppressive effects and its underling mechanism of miR-335 on colorectal cancer cells. Human colorectal cancer HCT116 cells were employed and the expression of miR-335 in cells was altered by transfection with miR-335 mimic and miR-335 inhibitor. Thereafter, CCK-8 assay, flow cytometry, Transwell assay and Western blotting were used to detect cell viability, apoptosis, migration, invasion, and the expression of epithelial-mesenchymal transition (EMT)-related proteins...
August 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441904/microrna-33a-deficiency-inhibits-proliferation-and-fibrosis-through-inactivation-of-tgf-%C3%AE-smad-pathway-in-human-cardiac-fibroblasts
#12
Bo Yu, Wei Li, Fen Al, Zhen Chen
Cardiac fibroblasts (CFBs) play pivotal roles in myocardial fibrosis, which is the leading cause of arrhythmia. This study was aimed to investigate the modulation of microRNA (miR)-33a on proliferation, apoptosis and fibrosis of human CFBs. CFBs were respectively transfected with miR-control, miR-33a mimic or miR-33a inhibitor, followed by induction of transforming growth factor-β (TGF-β). Non-treated CFBs acted as control. Cell viability, apoptosis, and fibrosis which reflected by expressions of Col-I, Col-III and α-smooth muscle actin (α-SMA) were evaluated by CCK-8 assay, flow cytometry, qRT-PCR and Western blot analysis...
August 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441874/serum-il-6-il-23-profile-and-treg-th17-peripheral-cell-populations-in-pediatric-patients-with-inflammatory-bowel-disease
#13
Xiao-Min Zhu, Ying-Zuo Shi, Ming Cheng, Da-Feng Wang, Jian-Feng Fan
IL-6 and IL-23 are both pleiotropic cytokines involved in the regulation of the immune response, inflammation, and hematopoeisis. They also could mediate effector cells and tolerance mediated by cells with regulatory function. Inflammatory bowel disease (IBD) is associated with a reduced ratio of Treg cells ato Th17 effector cells in peripheral blood and is characterised by a pro-inflammatory cytokine microenvironment which supports the continued generation of Th17 cells. It is well described in adults but little is known in a pediatric population...
May 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441873/microrna-190b-inhibits-tumor-cell-proliferation-and-induces-apoptosis-by-regulating-bcl-2-in-u2os-osteosarcoma-cells
#14
Mingyang Kang, Peng Xia, Tingting Hou, Zhiping Qi, Shaojun Liao, Xiaoyu Yang
Osteosarcoma (OS) is one of the most prevalent malignancies in bone with no established therapy so far. This study was aimed to clarify the role of miR-190b in tumor cell growth of OS. The miR-190b mimic, inhibitor and miR-control were transfected into human OS U2OS cells. Then U2OS cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and bromodeoxyuridine (BrdU) incorporation assay. The apoptotic U2OS cells were detected by flow cytometry. Additionally, cell-cycle regulators p27, p21 and apoptosis factors B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X (Bax), caspase-3 were examined by western blotting...
May 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441717/s100a9-gene-silencing-inhibits-the-release-of-pro-inflammatory-cytokines-by-blocking-the-il-17-signalling-pathway-in-mice-with-acute-pancreatitis
#15
Dong-Mei Wu, Shan Wang, Min Shen, Yong-Jian Wang, Bo Zhang, Zi-Qi Wu, Jun Lu, Yuan-Lin Zheng
The study aimed to investigate whether S100A9 gene silencing mediating the IL-17 pathway affected the release of pro-inflammatory cytokines in acute pancreatitis (AP). Kunming mice were assigned to the normal, AP, AP + negative control (NC), AP + shRNA, AP + IgG and AP + anti IL-17 groups. ELISA was applied to measure expressions of AMY, LDH, CRP, TNF-α, IL-6 and IL-8. The cells were distributed into the control, blank, NC, shRNA1 and shRNA2 groups. MTT assay, flow cytometry, RT-qPCR and Western blotting were used to evaluate cell proliferation, cell cycle and apoptosis, and expressions of S100A9, TLR4, RAGE, IL-17, HMGB1 and S100A12 in tissues and cells...
February 14, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29441681/sv40-intron-a-potent-strong-intron-element-that-effectively-increases-transgene-expression-in-transfected-chinese-hamster-ovary-cells
#16
Dan-Hua Xu, Xiao-Yin Wang, Yan-Long Jia, Tian-Yun Wang, Zheng-Wei Tian, Xin Feng, Yin-Na Zhang
Chinese hamster ovary (CHO) cells have become the most widely utilized mammalian cell line for the production of recombinant proteins. However, the product yield and transgene instability need to be further increased and solved. In this study, we investigated the effect of five different introns on transgene expression in CHO cells. hCMV intron A, adenovirus tripartite leader sequence intron, SV40 intron, Chinese hamster EF-1alpha gene intron 1 and intervening sequence intron were cloned downstream of the eGFP expression cassette in a eukaryotic vector, which was then transfected into CHO cells...
February 14, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29440916/overexpression-of-zwint-predicts-poor-prognosis-and-promotes-the-proliferation-of-hepatocellular-carcinoma-by-regulating-cell-cycle-related-proteins
#17
Hanning Ying, Zhiyao Xu, Mingming Chen, Senjun Zhou, Xiao Liang, Xiujun Cai
Introduction: Zwint, a centromere-complex component required for the mitotic spindle checkpoint, has been reported to be overexpressed in different human cancers, but it has not been studied in human hepatocellular carcinoma (HCC). Materials and methods: The role of Zwint in hepatocellular carcinoma cell proliferation capacities was evaluated by using cell counting kit-8 (CCK8), flow cytometry, clone formation and tumor formation assay in nude mice. Western blot analysis and qPCR assay were performed to assess Zwint interacting with cell-cycle-related proteins...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29440896/intranasal-delivery-of-huperzine-a-to-the-brain-using-lactoferrin-conjugated-n-trimethylated-chitosan-surface-modified-plga-nanoparticles-for-treatment-of-alzheimer-s-disease
#18
Qingqing Meng, Aiping Wang, Hongchen Hua, Ying Jiang, Yiyun Wang, Hongjie Mu, Zimei Wu, Kaoxiang Sun
Background: Safe and effective delivery of therapeutic drugs to the brain is important for successful therapy of Alzheimer's disease (AD). Purpose: To develop Huperzine A (HupA)-loaded, mucoadhesive and targeted polylactide-co-glycoside (PLGA) nanoparticles (NPs) with surface modification by lactoferrin (Lf)-conjugated N-trimethylated chitosan (TMC) (HupA Lf-TMC NPs) for efficient intranasal delivery of HupA to the brain for AD treatment. Methods: HupA Lf-TMC NPs were prepared using the emulsion-solvent evaporation method and optimized using the Box-Behnken design...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29440696/the-fate-of-a-hapten-from-the-skin-to-modification-of-macrophage-migration-inhibitory-factor-mif-in-lymph-nodes
#19
Isabella Karlsson, Kristin Samuelsson, Carl Simonsson, Anna-Lena Stenfeldt, Ulrika Nilsson, Leopold L Ilag, Charlotte Jonsson, Ann-Therese Karlberg
Skin (contact) allergy, the most prevalent form of immunotoxicity in humans, is caused by low molecular weight chemicals (haptens) that penetrate stratum corneum and modify endogenous proteins. The fate of haptens after cutaneous absorption, especially what protein(s) they react with, is largely unknown. In this study the fluorescent hapten tetramethylrhodamine isothiocyanate (TRITC) was used to identify hapten-protein conjugates in the local lymph nodes after topical application, as they play a key role in activation of the adaptive immune system...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440294/in-vitro-and-in-vivo-activity-of-imgn853-an-antibody-drug-conjugate-targeting-folate-receptor-alpha-linked-to-dm4-in-biologically-aggressive-endometrial-cancers
#20
Gary Altwerger, Elena Bonazzoli, Stefania Bellone, Tomomi Egawa-Takata, Gulden Menderes, Francesca Pettinella, Anna Bianchi, Francesco Riccio, Jacqueline Feinberg, Luca Zammataro, Chanhee Han, Ghanshyam Yadav, Katherine Dugan, Ashley Morneault, Jose F Ponte, Natalia Buza, Pei Hui, Serena Wong, Babak Litkouhi, Elena Ratner, Dan-Arin Silasi, Gloria S Huang, Masoud Azodi, Peter E Schwartz, Alessandro D Santin
Grade 3 endometrioid and uterine serous carcinomas (USC) account for the vast majority of endometrial cancer deaths. The purpose of this study was to determine folic acid receptor alpha (FR⍺) expression in these biologically aggressive (Type II) endometrial cancers,and evaluate FR⍺ as a targetable receptor for IMGN853 (Mirvetuximab soravtansine). The expression of FR⍺ was evaluated by immunohistochemistry (IHC) and flow cytometry in 90 endometrioid and USC samples. The in vitro cytotoxic activity and bystander effect were studied in primary uterine cancer cell lines expressing differential levels of FR⍺...
February 13, 2018: Molecular Cancer Therapeutics
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