keyword
MENU ▼
Read by QxMD icon Read
search

BMP-7

keyword
https://www.readbyqxmd.com/read/29789443/low-intensity-pulsed-ultrasound-stimulation-facilitates-in-vitro-osteogenic-differentiation-of-human-adipose-derived-stem-cells-via-up-regulation-of-heat-shock-protein-hsp-70-hsp90-and-bone-morphogenetic-protein-bmp-signaling-pathway
#1
Zhonglei Zhang, Yalin Ma, Shaowen Guo, Yi He, Gang Bai, Wenjun Zhang
Low-intensity pulsed ultrasound (LIPUS) has positive effects on osteogenic differentiation. However, the effect of LIPUS on osteogenic differentiation of human adipose-derived stem cells (hASCs) is unclear. In the present study, we investigated whether LIPUS could promote the proliferation and osteogenic differentiation of hASCs. hASCs were isolated and osteogenically induced with LIPUS stimulation at 20 and 30 mW cm-2 for 30 min day-1 Cell proliferation and osteogenic differentiation potential of hASCs were respectively analyzed by cell counting kit-8 assay, Alizarin Red S staining, real-time polymerase chain reaction, and Western blotting...
May 29, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29782946/delivery-of-the-improved-bmp-2-advanced-plasmid-dna-within-a-gene-activated-scaffold-accelerates-mesenchymal-stem-cell-osteogenesis-and-critical-size-defect-repair
#2
Rosanne M Raftery, Irene Mencía-Castaño, Simon Sperger, Gang Chen, Brenton Cavanagh, Georg Feichtinger, Heinz Redl, Ara Hacobian, Fergal J O'Brien
Gene-activated scaffolds have been shown to induce controlled, sustained release of functional transgene both in vitro and in vivo. Bone morphogenetic proteins (BMPs) are potent mediators of osteogenesis however we found that the delivery of plasmid BMP-2 (pBMP-2) alone was not sufficient to enhance bone formation. Therefore, the aim of this study was to assess if the use of a series of modified BMP-2 plasmids could enhance the functionality of a pBMP-2 gene-activated scaffold and ultimately improve bone regeneration when implanted into a critical sized bone defect in vivo...
May 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29779014/current-insights-on-use-of-growth-factors-as-therapy-for-intervertebral-disc-degeneration
#3
Justin C Kennon, Mohamed E Awad, Norman Chutkan, John DeVine, Sadanand Fulzele
Chronic low back pain is a critical health problem and a leading cause of disability in aging populations. A major cause of low back pain is considered to be the degeneration of the intervertebral disc (IVD). Recent advances in therapeutics, particularly cell and tissue engineering, offer potential methods for inhibiting or reversing IVD degeneration, which have previously been impossible. The use of growth factors is under serious consideration as a potential therapy to enhance IVD tissue regeneration. We reviewed the role of chosen prototypical growth factors and growth factor combinations that have the capacity to improve IVD restoration...
May 19, 2018: Biomolecular Concepts
https://www.readbyqxmd.com/read/29771832/growth-factors-release-from-concentrated-growth-factors-effect-of-%C3%AE-tricalcium-phosphate-addition
#4
Veronica Bonazza, Christopher Hajistilly, Dinker Patel, Jatan Patel, Rita Woo, Marco Angelo Cocchi, Barbara Buffoli, Davide Lancini, Ezio Gheno, Rita Rezzani, Sadia Jahanzeb, Adrian Hunnisett, Maher Almasri, Alberta Greco Lucchina, Matteo Brucoli, Carmen Mortellaro, Luigi Fabrizio Rodella
BACKGROUND: Platelet concentrates represent a new approach to improve tissue regeneration and can be used alone or together with autogenous bone, recombinant human growth factors, and/or other biomaterials, to enhance tissue regeneration. Among platelet concentrates, concentrated growth factors (CGFs) exhibit an interesting clinical and biotechnological application potential. OBJECTIVE: The aim of this study was to evaluate the in vitro release of 4 growth factors (bone morphogenetic proteins [BMP] -2, BMP-7, transforming growth factor [TGF] -β1, and insulin-like growth factor [IGF] -1) by the enzyme-linked immunosorbent assay (ELISA) technique, in CGFs mixed or not with β-tricalcium phosphate (β-TCP), using or not the Round-up device, at different times...
May 15, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29733462/current-and-potential-therapeutic-strategies-for-the-management-of-vascular-calcification-in-patients-with-chronic-kidney-disease-including-those-on-dialysis
#5
REVIEW
Irene Ruderman, Stephen G Holt, Tim D Hewitson, Edward R Smith, Nigel D Toussaint
Patients with CKD have accelerated vascular stiffening contributing significantly to excess cardiovascular morbidity and mortality. Much of the arterial stiffening is thought to involve vascular calcification (VC), but the pathogenesis of this phenomenon is complex, resulting from a disruption of the balance between promoters and inhibitors of calcification in a uremic milieu, along with derangements in calcium and phosphate metabolic pathways. Management of traditional cardiovascular risk factors to reduce VC may be influential but has not been shown to significantly improve mortality...
May 7, 2018: Seminars in Dialysis
https://www.readbyqxmd.com/read/29732529/osteostimulation-scaffolds-of-stem-cells-bmp-7-derived-peptide-decorated-alginate-porous-scaffolds-promote-the-aggregation-and-osteo-differentiation-of-human-mesenchymal-stem-cells
#6
Yue Yang, Zuyuan Luo, Ying Zhao
The scaffolds for stem cell-based bone tissue engineering should hold the ability to guide stem cells osteo-differentiating. Otherwise, stem cells will differentiate into unwanted cell types or will form tumors in vivo. Alginate, a natural polysaccharide with great biocompatibility, was widely used in biomedical applications. However, the limited bioactivity and poor osteogenesis capability of pristine alginate hampered its further application in tissue engineering. In this work, a bone forming peptide-1 (BFP-1), derived from bone morphogenetic protein-7, was grafted to alginate polymer chains to prepare peptide-decorated alginate porous scaffolds (pep-APS) for promoting osteo-differentiation of human mesenchymal stem cells (hMSCs)...
May 6, 2018: Biopolymers
https://www.readbyqxmd.com/read/29716947/embryonic-bone-morphogenetic-protein-and-nodal-induce-invasion-in-melanocytes-and-melanoma-cells
#7
Tobias Sinnberg, Heike Niessner, Mitch P Levesque, Christoph Dettweiler, Claus Garbe, Christian Busch
Despite recent progress in melanoma therapy via inhibition of activated oncogenes or immune stimulation, most stage IV melanoma patients still have limited survival times. Both therapeutic approaches eventually fail to prevent further invasion and metastasis, which is driven by a morphological process termed epithelial-mesenchymal transition (EMT). We previously demonstrated that inhibition of EMT in melanoma cells via antagonizing the bone morphogenetic protein (BMP)-pathway abrogated EMT and neural crest migration of melanoma cells in the chick embryo...
May 1, 2018: Biology Open
https://www.readbyqxmd.com/read/29713178/safety-study-is-there-a-pathologic-igf-1-pdgf-and-tgf-%C3%AE-cytokine-expression-caused-by-adjunct-bmp-7-in-tibial-and-femoral-non-union-therapy
#8
Christian Fischer, Christian Reiner, Gerhard Schmidmaier, Julian Doll, Christopher Child, Paul Alfred Grützner, Bahram Biglari, Sonja Boxriker, Arash Moghaddam
Background: In this prospective safety study, we investigated if the characteristic cytokine expression during bone regeneration is manipulated by the local application of bone morphogenetic protein-7 (BMP-7) in non-union surgery. Therefore, the levels of insulin like growth factor 1 (IGF-1), platelet-derived growth factor AB (PDGF-AB) and transforming growth factor beta (TGF-β) were compared between patients with the gold standard use of autologous bone graft (ABG) and those with additional application of BMP-7 as part of the diamond concept...
2018: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/29573911/limonene-promotes-osteoblast-differentiation-and-2-deoxy-d-glucose-uptake-through-p38mapk-and-akt-signaling-pathways-in-c2c12-skeletal-muscle-cells
#9
Ilavenil Soundharrajan, Da Hye Kim, Srigopalram Srisesharam, Palaniselvam Kuppusamy, Ravikumar Sivanesan, Ki Choon Choi
BACKGROUND: Limonene is a cyclic monoterpene (CTL) found in citrus fruits and many plant kingdoms. It has attracted attention as potential molecule due to its diverse biological activities. However, molecular mechanism involved in the osteogenic induction of CTL in C2C12 skeletal muscle cells remain unclear. PURPOSE: Skeletal development maintains the bone homeostasis through bone remodeling process. It coordinated between the osteoblast and osteoblast process. Osteoporosis is one of the most common bone diseases caused by a systemic reduction in bone mass...
March 12, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29565479/morphologic-and-molecular-alteration-during-tibia-fracture-healing-in-rat
#10
M-D Yu, B-H Su, X-X Zhang
OBJECTIVE: To monitor morphological feature and related osteogenic and bone metabolic change during healing of tibia fracture in a rat model. MATERIALS AND METHODS: Tibia density and trabecular thickness were evaluated. Histopathology was examined by HE staining. Serous inflammatory factors IL-4, IL-6, TNF-α and metabolic biomarkers ALP, β-CTX, P1NP, were determined by ELISA. The expression of RUNX2, TGF-β1, VEGF-α, BMP-2, BMP-4, and BMP-7 in callus tissue were qualified by RT-PCR...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29555587/bone-morphogenetic-protein-7-promotes-odontogenic-differentiation-of-dental-pulp-stem-cells-in-vitro
#11
Lei Zhu, Juan Ma, Rui Mu, Ruiqiao Zhu, Feng Chen, Xiaocui Wei, Xiaolei Shi, Shengqi Zang, Lei Jin
AIMS: in vitro effects of bone morphogenetic protein 7 (BMP-7) on proliferation and differentiation of dental pulp stem cells (DPSCs) have not been investigated, nor has an appropriate dose been established. MAIN METHODS: Human DPSCs obtained from healthy volunteers were cultured with BMP-7 at 25, 50, and 100 ng/ml. Cell viability was measured by Cell Counting Kit-8 assay. Expression profiles of selected odontogenic differentiation-related markers in DPSCs were evaluated using quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunocytochemistry, and western blot analysis...
June 1, 2018: Life Sciences
https://www.readbyqxmd.com/read/29545164/interaction-between-orexin-a-and-bone-morphogenetic-protein-system-on-progesterone-biosynthesis-by-rat-granulosa-cells
#12
Shiho Fujita, Toru Hasegawa, Yuki Nishiyama, Satoshi Fujisawa, Yasuhiro Nakano, Takahiro Nada, Nahoko Iwata, Yasuhiko Kamada, Hisashi Masuyama, Fumio Otsuka
The involvement of orexins in reproductive function has been gradually uncovered. However, the functional role of orexins in ovarian steroidogenesis remains unclear. In the present study, we investigated the effects of orexin A on ovarian steroidogenesis by using rat primary granulosa cells that express both OX1 and OX2 receptors for orexins. Treatment with orexin A enhanced progesterone, but not estradiol, biosynthesis induced by FSH, whereas it did not affect basal levels of progesterone or estradiol. In accordance with the effects on steroidogenesis, orexin A increased the mRNA levels of progesterogenic enzymes, including StAR, P450scc and 3βHSD, but not P450arom, and cellular cAMP synthesis induced by FSH...
March 12, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29512787/bmp7-regulates-lung-fibroblast-proliferation-in-newborn-rats-with-bronchopulmonary-dysplasia
#13
Yanli Sun, Jianhua Fu, Xindong Xue, Haiping Yang, Linlin Wu
The present study investigated the expression of bone morphogenetic protein (BMP) 7 in a newborn rat model of bronchopulmonary dysplasia (BPD) and the biological effects of BMP7 on newborn rat lung fibroblast (LF) cells. For this purpose, a total of 196 newborn rats were randomly and equally assigned to a model group and a control group. Lung tissue was collected at days 3, 7, 14 and 21 for histological analysis. The location and expression of BMP7 was examined by immunohistochemical staining and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis...
May 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29511371/bisphosphonate-modulation-of-the-gene-expression-of-different-markers-involved-in-osteoblast-physiology-possible-implications-in-bisphosphonate-related-osteonecrosis-of-the-jaw
#14
Francisco Javier Manzano-Moreno, Javier Ramos-Torrecillas, Lucia Melguizo-Rodríguez, Rebeca Illescas-Montes, Concepción Ruiz, Olga García-Martínez
The aim of the present study was to elucidate the role of osteoblasts in bisphosphonates-related osteonecrosis of the jaw (BRONJ). The specific objective was to evaluate the effect on osteoblasts of two nitrogen-containing BPs (zoledronate and alendronate) and one non-nitrogen-containing BP (clodronate) by analyzing modulations in their expression of genes essential for osteoblast physiology. Real-time polymerase chain reaction (RT-PCR) was used to study the effects of zoledronate, alendronate, and clodronate at doses of 10-5 , 10-7 , or 10-9 M on the expression of Runx-2, OSX, ALP, OSC, OPG, RANKL, Col-I, BMP-2, BMP-7, TGF-β1, VEGF, TGF-βR1, TGF-βR2, and TGF-βR3 by primary human osteoblasts (HOBs) and MG-63 osteosarcoma cells...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29509237/do-the-trace-elements-play-a-role-in-the-etiopathogenesis-of-developmental-dysplasia-of-hip
#15
S Guner, S I Guner, M A Gokalp, M F Ceylan, S S Unsal, H Demir
OBJECTIVE: Alterations in the connective tissue of the hip joint capsule and ligaments might account for the increased laxity seen in patients with developmental dysplasia of the hip. The tensile features of the connective tissue depend on collagen. A number of prior studies have noted the association between the trace elements and collagen biosynthesis. The aim of this research is to determine whether there exists an association between the trace elements and developmental dysplasia of the hip...
February 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29509236/the-application-of-prp-combined-with-tcp-in-repairing-avascular-necrosis-of-the-femoral-head-after-femoral-neck-fracture-in-rabbit
#16
X-L Zhang, Y-M Wang, K Chu, Z-H Wang, Y-H Liu, L-H Jiang, X Chen, Z-Y Zhou, G Yin
OBJECTIVE: In view of the high occurrence of avascular necrosis of the femoral head (ANFH) after femoral neck fracture and the difficulties in the treatment, our work aimed to explore the effects of platelet-rich plasma (PRP) combined with tri-calcium phosphate (TCP) on the repair of ANFH after femoral neck fracture and to provide reference for clinical treatment. MATERIALS AND METHODS: Thirty New Zealand white rabbits were randomly divided into control group, TCP group, and PRP+TCP group...
February 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29490276/p2ry1-alk3-expressing-cells-within-the-adult-human-exocrine-pancreas-are-bmp-7-expandable-and-exhibit-progenitor-like-characteristics
#17
Mirza Muhammad Fahd Qadir, Silvia Álvarez-Cubela, Dagmar Klein, Giacomo Lanzoni, Carlos García-Santana, Abelardo Montalvo, Fabiola Pláceres-Uray, Emilia Maria Cristina Mazza, Camillo Ricordi, Luca Alessandro Inverardi, Ricardo Luis Pastori, Juan Domínguez-Bendala
Treatment of human pancreatic non-endocrine tissue with Bone Morphogenetic Protein 7 (BMP-7) leads to the formation of glucose-responsive β-like cells. Here, we show that BMP-7 acts on extrainsular cells expressing PDX1 and the BMP receptor activin-like kinase 3 (ALK3/BMPR1A). In vitro lineage tracing indicates that ALK3+ cell populations are multipotent. PDX1+ /ALK3+ cells are absent from islets but prominently represented in the major pancreatic ducts and pancreatic duct glands. We identified the purinergic receptor P2Y1 (P2RY1) as a surrogate surface marker for PDX1...
February 27, 2018: Cell Reports
https://www.readbyqxmd.com/read/29445961/application-of-combined-porous-tantalum-scaffolds-loaded-with-bone-morphogenetic-protein-7-to-repair-of-osteochondral-defect-in-rabbits
#18
Qian Wang, Hui Zhang, Hongquan Gan, Hui Wang, Qijia Li, Zhiqiang Wang
PURPOSE: Porous tantalum (PT) has been widely used in orthopaedic applications for low modulus of elasticity, excellent biocompatibility, and the microstructures similar to cancellous bone. In order to improve the biological activity of PT, biologically active factors can be combined with the material. The purpose of this study was to investigate if bone morphogenetic protein 7 (BMP-7) modifications could enhance the repairing of cartilage of PT in osteochondral defect in medial femoral condyle of rabbits...
February 14, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29435112/lncrna-malat1-regulates-smooth-muscle-cell-phenotype-switch-via-activation-of-autophagy
#19
Tie-Feng Song, Li-Wen Huang, Ying Yuan, Hui-Qin Wang, Hong-Peng He, Wen-Jian Ma, Li-Hong Huo, Hao Zhou, Nan Wang, Tong-Cun Zhang
Vascular smooth muscle cells (VSMCs), switching from a differentiated to a proliferative phenotype, contribute to various vascular diseases. However, the role of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 MALAT1 in the phenotype switching of VSMCs remains unclear. Here, we report that the knockdown of MALAT1 promotes the transformation of smooth muscle cells from a proliferative phenotype to a differentiated phenotype. MALAT1 knockdown inhibited cellular proliferation and migration, leading to significant cell cycle arrest in the G2 phase...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29405371/disclosing-the-physiology-of-pulp-tissue-for-vital-pulp-therapy
#20
REVIEW
W L O da Rosa, E Piva, A F da Silva
The discovery that dentine is a reservoir of bioactive molecules that can be recruited on demand has attracted efforts to develop new protocols and materials for vital pulp therapy (VPT). The noncollagenous proteins (NCPs) present in the dentine extracellular matrix (ECM) include growth factors (TGF-β1, BMP-7, FGF-2, IGF-1 and IGF-2, NGF and GDNF), extracellular matrix molecules (DSP, DPP, BSP, DMP-1 and DSPP) and both anti-inflammatory and pro-inflammatory chemokines and cytokines (TNF-α, IL-1, IL-6 and IL-10)...
February 6, 2018: International Endodontic Journal
keyword
keyword
59318
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"