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https://www.readbyqxmd.com/read/28830033/quartz-crystal-microbalance-sensing-cell-substrate-adhesion-and-beyond
#1
REVIEW
Jennifer Y Chen, Lynn S Penn, Jun Xi
Cell adhesion is an essential aspect of cellular behavior. Finding innovative methods to probe the adhesion of cells in their native state can greatly advance the understanding of control and regulation of cellular behavior and their impact on human health. The quartz crystal microbalance (QCM) is a label-free, biosensing system that has, in the past fifty years, evolved from a simple acoustic based mass sensor to a powerful bioanalytical tool. Its unique capability of monitoring the cell-substrate interaction non-invasively in real time has led to the emergence of its applications in areas that are relevant to fundamental cell biology and medical research...
August 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28830009/humanization-of-jaa-f11-a-highly-specific-anti-thomsen-friedenreich-pancarcinoma-antibody-and-invitro-efficacy-analysis
#2
Swetha Tati, John C Fisk, Julia Abdullah, Loukia Karacosta, Taylor Chrisikos, Padraic Philbin, Susan Morey, Diala Ghazal, Fatma Zazala, Joseph Jessee, Sally Quataert, Stephen Koury, David Moreno, Jing Ying Eng, Vladislav V Glinsky, Olga V Glinskii, Muctarr Sesay, Anthony W Gebhard, Karamveer Birthare, James R Olson, Kate Rittenhouse-Olson
JAA-F11 is a highly specific mouse monoclonal to the Thomsen-Friedenreich Antigen (TF-Ag) which is an alpha-O-linked disaccharide antigen on the surface of ~80% of human carcinomas, including breast, lung, colon, bladder, ovarian, and prostate cancers, and is cryptic on normal cells. JAA-F11 has potential, when humanized, for cancer immunotherapy for multiple cancer types. Humanization of JAA-F11, was performed utilizing complementarity determining regions grafting on a homology framework. The objective herein is to test the specificity, affinity and biology efficacy of the humanized JAA-F11 (hJAA-F11)...
August 19, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28829967/cancer-biology-apc-delivers-kiss-of-death-to-focal-adhesions
#3
Inke Näthke
New work shows that the actin-nucleating ability of the adenomatous polyposis coli protein is required for disassembly of focal adhesions. Loss of this function in Apc mutant cells reduces directed cell migration, potentially explaining the decreased migration of colon cancer cells.
August 21, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28829939/protein-clustering-shapes-polarity-protein-gradients
#4
Edwin Munro
In this issue of Developmental Cell, Dickinson et al. (2017) and Rodriguez et al. (2017), along with Wang et al. (2017) in Nature Cell Biology, show how PAR protein oligomerization can dynamically couple protein diffusion and transport by cortical flow to control kinase activity gradients and polarity in the C. elegans zygote.
August 21, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28829890/mir-367-promotes-uveal-melanoma-cell-proliferation-and-migration-by-regulating-pten
#5
J W Ling, P R Lu, Y B Zhang, S Jiang, Z C Zhang
We aimed to investigate the biological role of miR-367 in uveal melanoma cell growth and migration, and the underlying mechanism responsible. Quantitative real-time polymerase chain reaction was performed to evaluate miR-367 expression in uveal melanoma tissue samples and cell lines. A miR-367 mimic, miR-367 inhibitor, and negative control oligonucleotide were transfected into these cells to investigate the function of this microRNA. In addition, the role of PTEN in miR-367-mediated uveal melanoma cell growth and migration was evaluated...
August 17, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28829832/allergen-homologs-in-the-euroglyphus-maynei-draft-genome
#6
S Dean Rider, Marjorie S Morgan, Larry G Arlian
Euroglyphus maynei is a house dust mite commonly found in homes worldwide and is the source of allergens that sensitize and induce allergic reactions in humans. It is the source of species-specific allergens as well as allergens that are cross-reactive with the allergens from house dust mites Dermatophagoides farinae and D. pteronyssinus, and the ectoparasitic scabies mite Sarcoptes scabiei. The genomics, proteomics and molecular biology of E. maynei and its allergens have not been as extensively investigated as those of D...
2017: PloS One
https://www.readbyqxmd.com/read/28829774/a-large-scale-plasmodium-vivax-saimiri-boliviensis-trophozoite-schizont-transition-proteome
#7
D C Anderson, Stacey A Lapp, John W Barnwell, Mary R Galinski
Plasmodium vivax is a complex protozoan parasite with over 6,500 genes and stage-specific differential expression. Much of the unique biology of this pathogen remains unknown, including how it modifies and restructures the host reticulocyte. Using a recently published P. vivax reference genome, we report the proteome from two biological replicates of infected Saimiri boliviensis host reticulocytes undergoing transition from the late trophozoite to early schizont stages. Using five database search engines, we identified a total of 2000 P...
2017: PloS One
https://www.readbyqxmd.com/read/28829698/the-pro-%C3%AE-1-v-collagen-gene-col5a1-is-coordinately-regulated-by-mir-29b-with-core-promoter-in-cultured-cells
#8
Juan Juan Zhang, Hiroyuki Yano, Takako Sasaki, Noritaka Matsuo, Hidekatsu Yoshioka
AIMS: Col5a1 encodes the α1 chain of type V collagen, a quantitatively minor fibrillar collagen that is critical for the formation and function of the organs in the body. MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate biological functions by binding to the 3'- untranslated region (3'UTR) of specific target mRNA. In this study, we investigated the post-transcriptional regulation of miRNAs on the Col5a1 gene expression. MATERIALS AND METHODS: We cultured osteoblasts and fibroblasts of cell lines...
August 22, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28829673/oxygen-enhancement-ratio-in-radiation-induced-initial-dsbs-by-an-optimized-flow-cytometry-based-gamma-h2ax-analysis-in-a549-human-cancer-cells
#9
Shigeaki Sunada, Hirokazu Hirakawa, Akira Fujimori, Mitsuru Uesaka, Ryuichi Okayasu
High-linear energy transfer (LET) heavy ions cause higher therapeutic effects than low-LET radiation due to lower dependency on oxygen concentration in tumor cell killing. The lethality after irradiation largely depends on DNA double-strand breaks (DSBs), however the detailed LET dependency for DSB induction under oxic and hypoxic conditions has not been reported. Therefore, we evaluated the oxygen enhancement ratio (OER) of heavy ion-induced DSB induction using a highly-optimized flow cytometry-based method of γ-H2AX detection...
August 22, 2017: Radiation Research
https://www.readbyqxmd.com/read/28829599/nonpyrogenic-molecular-adjuvants-based-on-norabu-muramyldipeptide-and-norabu-glucosaminyl-muramyldipeptide-synthesis-molecular-mechanisms-of-action-biological-activities-in-vitro-and-in-vivo
#10
Roman Effenberg, Pavlína Turánek Knötigová, Daniel Zyka, Hana Čelechovská, Josef Mašek, Eliška Bartheldyová, Frantisek Hubatka, Štěpán Koudelka, Róbert Lukáč, Anna Kovalová, David Saman, Michal Křupka, Lucia Barkocziová, Petr Kosztyu, Marek Šebela, Ladislav Drož, Michal Hučko, Mária Kanásová, Andrew David Miller, Milan Raška, Miroslav Ledvina, Jaroslav Turanek
Apyrogenic fatty acyl analogues of muramyldipeptide (MDP) (abbreviated N-L18norAbuGMDP, N-B30norAbuGMDP, norAbuMDP-Lys(L18), norAbuMDP-Lys(B30), norAbuGMDP-Lys(L18), norAbuGMDP-Lys(B30), (B30)norAbuMDP, (L18)norAbuMDP) were designed and synthesized comprising the normuramyl-L-α-aminobutanoyl (norAbu) structural moiety. All new analogues show depressed pyrogenicity both as free compounds and in liposomal formulations when tested in vivo in rabbits (s.c. and i.v. application). The potency of analogues in selective activation of NOD2 and NLRP3 (inflammasome) receptors in vitro, but not NOD1, is demonstrated using transgenic cell-based detection systems...
August 22, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28829562/facile-fabrication-and-characterization-of-a-pdms-derived-candle-soot-coated-stable-biocompatible-superhydrophobic-and-superhemophobic-surface
#11
Rameez Iqbal, Butunath Majhy, Ashis K Sen
We report a simple, inexpensive, rapid and one-step method for the fabrication of a stable and biocompatible superhydrophobic and superhemophobic surface. The proposed surface comprises candle soot particles embedded in a mixture of PDMS+n-hexane serving as the base material. The mechanism responsible for the superhydrophobic behavior of the surface is explained and the surface is characterized based on its morphology and elemental composition, wetting properties, mechanical and chemical stability and biocompatibility...
August 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28829503/microrna-124-inhibits-cell-invasion-and-epithelial-mesenchymal-transition-by-directly-repressing-snail2-in-gastric-cancer
#12
S-L Li, H-L Gao, X-K Lv, Y-R Hei, P-Z Li, J-X Zhang, N Lu
OBJECTIVE: MicroRNAs (miRNAs) act as critical regulators of genes expression involved in tumor biological processes. The study aimed to investigate the clinical significance and biological role of miR-124 in gastric cancer (GC). PATIENTS AND METHODS: MiR-124 expression was analyzed from 88 GC tissues and adjacent normal tissues by quantitative Real-time PCR (qRT-PCR). Kaplan-Meier curves and log-rank test was used to evaluate the association between miR-124 and the over survival (OS) time of GC patients...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829501/promotion-of-lncrna-hoxa11-as-on-the-proliferation-of-hepatocellular-carcinoma-by-regulating-the-expression-of-lats1
#13
J Yu, J-F Hong, J Kang, L-H Liao, C-D Li
OBJECTIVE: To investigate the expression levels of lncRNA HOXA11-AS in HCC tissues and cells, and to explore its biological role in the development and progression of HCC. PATIENTS AND METHODS: We detected the relative expression level of HOXA11-AS in 72 HCC tissues and cells by the real-time quantitative PCR (qRT-PCR) assay. After interference with HOXA11-AS expression in HCC cells, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), clone formation, flow cytometry and an established nude mice transplanted tumor model were used to detect the biological behavior of HCC cells...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829500/promotion-of-proliferation-and-metastasis-of-hepatocellular-carcinoma-by-lncrna00673-based-on-the-targeted-regulation-of-notch-signaling-pathway
#14
H Chen, J-Z Liu, G-J Hu, L-L Shi, T Lan
OBJECTIVE: To investigate the relative expression of long non-coding RNA 00673 (lncRNA 00673) in hepatocellular carcinoma (HCC) and HCC cells and study its regulation on the malignant phenotype of HCC cells PATIENTS AND METHODS: Samples of HCC and adjacent tissues from January 2013 to December 2015 were collected. The expression level of lncRNA00673 in HCC tissues and cells was detected by quantitative Real-time polymerase chain reaction (qRT-PCR) assays. lncRNA00673 specific interference sequences were transiently transfected into HCC cells and the effect of HCC cells on the biological behavior of HCC cells was examined by in vitro experiments ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay), flow cytometry, transwell, etc...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829444/gene-regulation-in-the-immune-system-by-long-noncoding-rnas
#15
REVIEW
Y Grace Chen, Ansuman T Satpathy, Howard Y Chang
Long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression in the immune system. Studies have shown that lncRNAs are expressed in a highly lineage-specific manner and control the differentiation and function of innate and adaptive cell types. In this Review, we focus on mechanisms used by lncRNAs to regulate genes encoding products involved in the immune response, including direct interactions with chromatin, RNA and proteins. In addition, we address new areas of lncRNA biology, such as the functions of enhancer RNAs, circular RNAs and chemical modifications to RNA in cellular processes...
August 22, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28829423/multi-photon-time-lapse-imaging-to-visualize-development-in-real-time-visualization-of-migrating-neural-crest-cells-in-zebrafish-embryos
#16
Antionette L Williams, Brenda L Bohnsack
Congenital eye and craniofacial anomalies reflect disruptions in the neural crest, a transient population of migratory stem cells that give rise to numerous cell types throughout the body. Understanding the biology of the neural crest has been limited, reflecting a lack of genetically tractable models that can be studied in vivo and in real-time. Zebrafish is a particularly important developmental model for studying migratory cell populations, such as the neural crest. To examine neural crest migration into the developing eye, a combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time videos of the developing eye in transgenic zebrafish embryos, namely Tg(sox10:EGFP) and Tg(foxd3:GFP), as sox10 and foxd3 have been shown in numerous animal models to regulate early neural crest differentiation and likely represent markers for neural crest cells...
August 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28829420/measuring-mrna-levels-over-time-during-the-yeast-s-cerevisiae-hypoxic-response
#17
Stephen D Willis, A K M Nawshad Hossian, Nathan Evans, Mark J Hickman
Complex changes in gene expression typically mediate a large portion of a cellular response. Each gene may change expression with unique kinetics as the gene is regulated by the particular timing of one of many stimuli, signaling pathways or secondary effects. In order to capture the entire gene expression response to hypoxia in the yeast S. cerevisiae, RNA-seq analysis was used to monitor the mRNA levels of all genes at specific times after exposure to hypoxia. Hypoxia was established by growing cells in ~100% N2 gas...
August 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28829416/quantitative-localization-of-a-golgi-protein-by-imaging-its-center-of-fluorescence-mass
#18
Hieng Chiong Tie, Bing Chen, Xiuping Sun, Li Cheng, Lei Lu
The Golgi complex consists of serially stacked membrane cisternae which can be further categorized into sub-Golgi regions, including the cis-Golgi, medial-Golgi, trans-Golgi and trans-Golgi network. Cellular functions of the Golgi are determined by the characteristic distribution of its resident proteins. The spatial resolution of conventional light microscopy is too low to resolve sub-Golgi structure or cisternae. Thus, the immuno-gold electron microscopy is a method of choice to localize a protein at the sub-Golgi level...
August 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28829412/identification-of-plasmodesmal-localization-sequences-in-proteins-in-planta
#19
Cheng Yuan, Sondra G Lazarowitz, Vitaly Citovsky
Plasmodesmata (Pd) are cell-to-cell connections that function as gateways through which small and large molecules are transported between plant cells. Whereas Pd transport of small molecules, such as ions and water, is presumed to occur passively, cell-to-cell transport of biological macromolecules, such proteins, most likely occurs via an active mechanism that involves specific targeting signals on the transported molecule. The scarcity of identified plasmodesmata (Pd) localization signals (PLSs) has severely restricted the understanding of protein-sorting pathways involved in plant cell-to-cell macromolecular transport and communication...
August 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28829369/systemic-chemokine-levels-with-gut-specific-vedolizumab-in-patients-with-inflammatory-bowel-disease-a-pilot-study
#20
Stephanie Zwicker, Ronaldo Lira-Junior, Charlotte Höög, Sven Almer, Elisabeth A Boström
Vedolizumab, a gut-specific biological treatment for inflammatory bowel disease (IBD), is an antibody that binds to the α₄β₇ integrin and blocks T-cell migration into intestinal mucosa. We aimed to investigate chemokine levels in serum of IBD-patients treated with vedolizumab. In this pilot study, we included 11 IBD patients (8 Crohn's disease, 3 ulcerative colitis) previously non-respondent to anti-tumor necrosis factor (TNF)-agents. Patients received vedolizumab at week 0, 2 and 6 and were evaluated for clinical efficacy at week 10...
August 22, 2017: International Journal of Molecular Sciences
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