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Cu Zn superoxide dismutase

Gangduo Wang, Silvia S Pierangeli, Rohan Willis, Emilio B Gonzalez, Michelle Petri, M Firoze Khan
Even though systemic lupus erythematosus (SLE) is associated with high morbidity and mortality rates among young and middle-aged women, the molecular mechanisms of disease pathogenesis are not fully understood. Previous studies from our laboratory suggested an association between oxidative stress and SLE disease activity (SLEDAI). To further assess the role of reactive oxygen species (ROS) in SLE, we examined the contribution of lipid-derived reactive aldehydes (LDRAs)-specific immune complexes in SLE. Sera from 60 SLE patients with varying SLEDAI and 32 age- and gender- matched healthy controls were analyzed for oxidative stress and related markers...
2016: PloS One
Soheila Asadi, Mohammad Nabi Moradi, Nejat Khyripour, Mohammad Taghi Goodarzi, Marzieh Mahmoodi
Diabetes is a common metabolic disorder characterized by elevated blood glucose level. Trace element homeostasis causes disturbances in diabetes due to hyperglycemia. Superoxide dismutase (SOD), an antioxidant enzyme, contains zinc and copper ions as its cofactors. Defects in SOD level and activity have been observed in diabetes. Resveratrol (RSV) has displayed hypoglycemic effects and is proven to improve oxidative stress. The aim of the present study was to examine the possible effects of RSV on blood glucose level, serum copper and zinc levels, SOD, and a number of other oxidative markers in type 2 diabetic rats...
October 15, 2016: Biological Trace Element Research
Huan Zhao, Wanjuan Li, Xinda Zhao, Xu Li, Dazuo Yang, Hongwei Ren, Yibing Zhou
Cu/Zn superoxide dismutase (SOD) and catalase (CAT) cDNAs from the polychaete Perinereis aibuhitensis were cloned and characterized in order to investigate the relationship between crude oil exposure and stress response in this worm. The full length of PaSOD was 870 bp and PaCAT was 1967 bp encoding 150 and 506 amino acids, respectively. Gene expression and enzyme activity of Cu/Zn SOD and CAT in response to crude oil contaminated soil (500, 1500, and 3000 mg/kg) were measured. The results showed that expression of the CAT gene and enzyme activity in P...
October 14, 2016: Environmental Science and Pollution Research International
Dae Young Yoo, Dae Won Kim, Jin Young Chung, Hyo Young Jung, Jong Whi Kim, Yeo Sung Yoon, In Koo Hwang, Jung Hoon Choi, Goang-Min Choi, Soo Young Choi, Seung Myung Moon
In the present study, we investigated the ability of Cu, Zn-superoxide dismutase (SOD1) to improve the therapeutic potential of adipose tissue-derived mesenchymal stem cells (Ad-MSCs) against ischemic damage in the spinal cord. Animals were divided into four groups: the control group, vehicle (PEP-1 peptide and artificial cerebrospinal fluid)-treated group, Ad-MSC alone group, and Ad-MSC-treated group with PEP-1-SOD1. The abdominal aorta of the rabbit was occluded for 30 min in the subrenal region to induce ischemic damage, and immediately after reperfusion, artificial cerebrospinal fluid or Ad-MSCs (2 × 10(5)) were administered intrathecally...
October 14, 2016: Neurochemical Research
Jacob I Ayers, Benjamin McMahon, Sabrina Gill, Herman L Lelie, Susan Fromholt, Hilda Brown, Joan Selverstone Valentine, Julian P Whitelegge, David R Borchelt
A common property of Cu/Zn superoxide dismutase 1 (SOD1), harboring mutations associated with amyotrophic lateral sclerosis (ALS), is a high propensity to misfold and form abnormal aggregates. The aggregation of mutant SOD1 has been demonstrated in vitro, with purified proteins, in mouse models, in human tissues, and in cultured cell models. In vitro translation studies have determined that SOD1 with ALS mutations is slower to mature, and thus perhaps vulnerable to off-pathway folding that could generate aggregates...
October 11, 2016: Journal of Neurochemistry
Yingli Yang, Ting Ma, Fan Ding, Haizhen Ma, Xiaohui Duan, Tianpeng Gao, Jian Yao
Growth inhibition and antioxidative response were investigated in wheat roots cultured in 1/4 Hoagland solution containing zinc (Zn, 500 μM), iron (Fe, 300 μM), and copper (Cu, 300 μM) in combination. Different Zn, Fe, and Cu interactions inhibited seedling growth and increased Zn, Fe, and Cu contents in roots and shoots, with the most significant inhibition due to Zn + Fe + Cu treatment. The elevation of malondialdehyde content and the loss of cell viability resulted from the increases of total and apoplastic hydrogen peroxide (H2O2) and hydroxyl radical (·OH) contents in all treated roots...
October 10, 2016: Environmental Science and Pollution Research International
Veeran Srinivasan, Periyakali Saravana Bhavan, Gopalan Rajkumar, Thangavelu Satgurunathan, Thirunavukkarasu Muralisankar
This study was performed to assess the growth-promoting potential of dietary magnesium oxide nanoparticles (MgO NPs) in Macrobrachium rosenbergii post-larvae (PL). MgO NPs were supplemented at 0, 100, 200, 300, 400 and 500 mg kg(-1) with the basal diet (containing 0.95 g Mg kg(-1)); the concentrations of Mg in MgO NP-supplemented diets were increased correspondingly (1.07, 1.15, 1.24, 1.37 and 1.46 g Mg kg(-1) respectively). MgO NP-supplemented diets were fed to M. rosenbergii PL (initial weight 0.11 ± 0...
October 5, 2016: Biological Trace Element Research
Alejandro Pezza, Lucas B Pontel, Carolina López, Fernando C Soncini
Copper homeostasis is essential for bacterial pathogen fitness and infection, and has been the focus of a number of recent studies. In Salmonella, envelope protection against copper overload and macrophage survival depends on CueP, a major copper-binding protein in the periplasm. This protein is also required to deliver the metal ion to the Cu/Zn superoxide dismutase SodCII. The Salmonella-specific CueP-coding gene was originally identified as part of the Cue regulon under the transcriptional control of the cytoplasmic copper sensor CueR, but its expression differs from the rest of CueR-regulated genes...
October 11, 2016: Proceedings of the National Academy of Sciences of the United States of America
Mashidur Rana, Sudhir C Roy, Bannur C Divyashree
The status of antioxidant defences of both spermatozoa and their associated fluids during epididymal transit from the caput to cauda have not been studied so far in any species. Herein we report for the first time that sperm antioxidant defences, namely Cu,Zn-superoxide dismutase (Cu,Zn-SOD) and catalase activity, decrease significantly (P<0.05) from the caput to cauda during epididymal transit in parallel with increases in Cu,Zn-SOD, total SOD and total glutathione peroxidase (GPx) activity in the luminal fluid of the respective segments...
September 28, 2016: Reproduction, Fertility, and Development
James M Fay, Cheng Zhu, Elizabeth A Proctor, Yazhong Tao, Wenjun Cui, Hengming Ke, Nikolay V Dokholyan
The majority of amyotrophic lateral sclerosis (ALS)-related mutations in the enzyme Cu,Zn superoxide dismutase (SOD1), as well as a post-translational modification, glutathionylation, destabilize the protein and lead to a misfolded oligomer that is toxic to motor neurons. The biophysical role of another physiological SOD1 modification, T2-phosphorylation, has remained a mystery. Here, we find that a phosphomimetic mutation, T2D, thermodynamically stabilizes SOD1 even in the context of a strongly SOD1-destabilizing mutation, A4V, one of the most prevalent and aggressive ALS-associated mutations in North America...
September 17, 2016: Structure
Jingbo Zhang, Bo Li, Yang Yang, Wenran Hu, Fangyuan Chen, Lixia Xie, Ling Fan
Superoxide dismutase (SOD) as a group of significant and ubiquitous enzymes plays a critical function in plant growth and development. Previously this gene family has been investigated in Arabidopsis and rice; it has not yet been characterized in cotton. In our study, it was the first time for us to perform a genome-wide analysis of SOD gene family in cotton. Our results showed that 10 genes of SOD gene family were identified in Gossypium arboreum and Gossypium raimondii, including 6 Cu-Zn-SODs, 2 Fe-SODs, and 2 Mn-SODs...
2016: International Journal of Genomics
Katarzyna Kowalska, Halina Milnerowicz
The aim of the present study was to examine the influence of age and gender on the pro/antioxidant balance in young healthy subject aged 20-40 years. The individuals were divided into women: aged 20-25 years (group A), aged 32-39 years (group B), and men aged 20-25 years (group C). In blood, the metals, markers of oxidative stress and antioxidants were estimated. The Cu level and Cu/Zn ratio in group B was higher than in group A. There were no differences in metals concentrations between groups A and C. The similar levels of malondialdehyde concentrations and gamma-glutamyl transpeptidase activities were observed in all groups...
September 2016: Annals of Clinical and Laboratory Science
Jia-Lang Zheng, Shuang-Shuang Yuan, Chang-Wen Wu, Zhen-Ming Lv
Cadmium (Cd) is an environmental contaminant that poses serious risks to aquatic organisms and their associated ecosystem. The mechanisms underlying Cd-induced oxidative stress and immunotoxicity in fish remain largely unknown. In this study, adult female zebrafish were exposed to 0 (control), 1mgL(-1) Cd for 24h and 96h, and the oxidative stress and inflammatory responses induced by Cd were evaluated in the brain, liver and ovary. Reactive oxygen species (ROS), nitric oxide (NO), and malondialdehyde (MDA) increased in a time-dependent manner after treatment with Cd in the brain and liver...
September 14, 2016: Aquatic Toxicology
Qian Wen, Yuzhi Wang, Kaijia Xu, Na Li, Hongmei Zhang, Qin Yang
A novel magnetic adsorbent, benzyl groups functionalized imidazolium-based polymeric ionic liquid (PIL)-coated magnetic multiwalled carbon nanotubes (MWCNTs) (m-MWCNTs@PIL), has been successfully synthesized and applied for the extraction of Cu, Zn-superoxide dismutase (Cu, Zn-SOD). The m-MWCNTs@PIL were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), vibrating sample magnetometer (VSM) and zeta-potential nanoparticles...
October 5, 2016: Analytica Chimica Acta
Israel Pérez-Torres, Verónica Guarner-Lans, Alejandra Zúñiga-Muñoz, Rodrigo Velázquez Espejel, Alfredo Cabrera-Orefice, Salvador Uribe-Carvajal, Natalia Pavón
We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR)...
2016: Oxidative Medicine and Cellular Longevity
Takamasa Ishii, Yumi Takanashi, Koichi Sugita, Masaki Miyazawa, Rintaro Yanagihara, Kayo Yasuda, Hiromi Onouchi, Noboru Kawabe, Munehiro Nakata, Yorihiro Yamamoto, Phil S Hartman, Naoaki Ishii
The etiology of astrocyte dysfunction is not well understood even though neuronal defects have been extensively studied in a variety of neuronal degenerative diseases. Astrocyte defects could be triggered by the oxidative stress that occurs during physiological aging. Here, we provide evidence that intracellular or mitochondrial reactive oxygen species (ROS) at physiological levels can cause hippocampal (neuronal) dysfunctions. Specifically, we demonstrate that astrocyte defects occur in the hippocampal area of middle-aged Tet-mev-1 mice with the SDHC(V69E) mutation...
September 13, 2016: Aging Cell
Gungor Sitar, Mehmet Kucuk, Mustafa Erinc Sitar, Ozgur Yasar, Seval Aydin, Karolin Yanar, Ufuk Cakatay, Nur Buyukpınarbasili
BACKGROUND: One of the most important side effects of contrast pharmaceutical agents, which are used very common in routine radiology practice, is contrast induced nephropathy. Even ischemia, oxidative stress and osmolality related cytotoxic effects are considered, the molecular mechanisms underlying this pathology have not been identified completely yet. OBJECTIVES: The aim of the current study was to reveal the role of oxidative stress and antioxidant enzymatic defence mechanisms in the aetiopathogenesis of contrast-induced nephropathy...
June 2016: Iranian Red Crescent Medical Journal
Raşit Kιlιç, Aslhan Cavunt Bayraktar, Serdar Bayraktar, Ali Kurt, Mustafa Kavutçu
PURPOSE: The aim of this study was to evaluate the relationship between antioxidant superoxide dismutase (SOD) enzyme activity, malondialdehyde (MDA) as a lipid peroxidation marker, and some trace elements such as zinc (Zn) and copper (Cu) levels in patients with keratoconus. METHODS: A total of 58 patients with keratoconus and 53 control subjects with similar age and sex were evaluated in this study. The modified Krumeich keratoconus classification was used to divide the patients into 4 stages...
September 8, 2016: Cornea
N C N Perera, G I Godahewa, Jehee Lee
Copper-zinc-superoxide dismutase (CuZnSOD) from Hippocampus abdominalis (HaCuZnSOD) is a metalloenzyme which belongs to the ubiquitous family of SODs. Here, we determined the characteristic structural features of HaCuZnSOD, analyzed its evolutionary relationships, and identified its potential immune responses and biological functions in relation to antioxidant defense mechanisms in the seahorse. The gene had a 5' untranslated region (UTR) of 67 bp, a coding sequence of 465 bp and a 3' UTR of 313 bp. The putative peptide consists of 154 amino acids...
October 2016: Fish & Shellfish Immunology
Matthew J Jenny, William C Walton, Samantha L Payton, John M Powers, Robert H Findlay, Britton O'Shields, Kirsten Diggins, Mark Pinkerton, Danielle Porter, Daniel M Crane, Jeffrey Tapley, Charles Cunningham
Estuarine organisms were impacted by the Deepwater Horizon oil spill which released ∼5 million barrels of crude oil into the Gulf of Mexico in the spring and summer of 2010. Crassostrea virginica, the American oyster, is a keystone species in these coastal estuaries and is routinely used for environmental monitoring purposes. However, very little is known about their cellular and molecular responses to hydrocarbon exposure. In response to the spill, a monitoring program was initiated by deploying hatchery-reared oysters at three sites along the Alabama and Mississippi coast (Grand Bay, MS, Fort Morgan, AL, and Orange Beach, AL)...
September 2016: Marine Environmental Research
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