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Cu Zn superoxide dismutase

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https://www.readbyqxmd.com/read/28819808/mir398-and-mir395-are-involved-in-response-to-so2-stress-in-arabidopsis-thaliana
#1
Lihong Li, Huilan Yi, Meizhao Xue, Min Yi
Sulfur dioxide (SO2) is a common air pollutant that has adverse effects on plants. MicroRNAs (miRNAs) are small noncoding RNA that play critical roles in plant development and stress response. In this study, we found that two miRNAs, miR398 and miR395, were differentially expressed in Arabidopsis shoots under SO2 stress. The expression of miR398 was down-regulated, and the transcript levels of its target genes, Cu/Zn superoxide dismutases (CSD1 and CSD2), were increased during SO2 exposure. The activity of superoxide dismutase (SOD), one of the major antioxidant enzymes, was enhanced with the increase in the CSD transcript level, suggesting an important role of miR398 in response to SO2-induced oxidative stress...
August 17, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28803025/the-effect-of-nano-tio2-photocatalysis-on-the-antioxidant-activities-of-cu-zn-sod-at-physiological-ph
#2
Wen Zheng, Hai-Feng Zou, Shao-Wu Lv, Yan-Hong Lin, Min Wang, Fei Yan, Ye Sheng, Yan-Hua Song, Jie Chen, Ke-Yan Zheng
Security issues of nanoparticles on biological toxicity and potential environmental risk have attracted more and more attention with the rapid development and wide applications of nanotechnology. In this work, we explored the effect and probable mechanism of nano-TiO2 on antioxidant activity of copper, zinc superoxide dismutase (Cu, Zn-SOD) under natural light and mixed light at physiological pH. Nano-TiO2 was prepared by sol-hydrothermal method, and then characterized by X-ray Diffraction (XRD) and Transmission electron micrographs (TEM)...
August 5, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28782426/solvent-sensitivity-of-protein-aggregation-in-cu-zn-superoxide-dismutase-a-molecular-dynamics-simulation-study
#3
Amresh Prakash, Vijay Kumar, Preeti Pandey, Deepak R Bharti, Poonam Vishwakarma, Ruhar Singh, Md Imtaiyaz Hassan, Andrew M Lynn
Misfolding and aggregation of Cu, Zn Superoxide Dismutase (SOD1) is often found in amyotrophic lateral sclerosis (ALS) patients. The central apo SOD1 barrel was involved in protein maturation and pathological aggregation in ALS. In this work, we employed atomistic molecular dynamics (MD) simulations to study the conformational dynamics of SOD1(barrel) monomer in different concentrations of trifluoroethanol (TFE). We find concentration dependence unusual structural and dynamical features, characterized by the local unfolding of SOD1(barrel)...
August 6, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28780408/an-essential-role-of-n-terminal-domain-of-copper-chaperone-in-the-enzymatic-activation-of-cu-zn-superoxide-dismutase
#4
Mami Fukuoka, Eiichi Tokuda, Kenta Nakagome, Zhiliang Wu, Isao Nagano, Yoshiaki Furukawa
Cu/Zn-superoxide dismutase (SOD1) is an enzyme that disproportionates superoxide anion into hydrogen peroxide and molecular oxygen. The enzymatic activity of SOD1 requires the binding of copper and zinc ions and also the formation of a conserved intramolecular disulfide bond. In a eukaryotic cell, a copper chaperone for SOD1 (CCS) has been known to supply a copper ion and also introduce the disulfide bond into SOD1; however, a mechanism controlling the CCS-dependent activation of SOD1 remains obscure. Here, we characterized CCS isolated from a human liver fluke, Clonorchis sinensis, and found that an N-terminal domain of CCS was essential in supplying a copper ion in SOD1...
July 31, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28777962/effect-of-graphene-oxide-on-copper-stress-in-lemna-minor-l-evaluating-growth-biochemical-responses-and-nutrient-uptake
#5
Changwei Hu, Lei Liu, Xiuling Li, Yundi Xu, Zhigang Ge, Yongjun Zhao
The wide application and unique properties of graphene oxide (GO) make it to interact with other pollutants and subsequently alter their behavior and toxicity. We evaluated the influences of GO at different concentrations (1 and 5mg/L) on copper (Cu) stress in duckweed (Lemna minor L.) GO below a concentration of 5mg/L showed no adverse effects on L. minor. The addition of Cu above 10μM represented a stress condition, which was evidenced by various parameters such as frond number, percent inhibition of growth rate (Ir), total chlorophyll content, dry weight, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)...
July 27, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28776286/bypassing-mirna-mediated-gene-regulation-under-drought-stress-alternative-splicing-affects-csd1-gene-expression
#6
So-Yon Park, Elizabeth Grabau
The binding site for miR398 in an isoform of Cu/Zn superoxide dismutase (CSD1) is eliminated by alternative splicing to bypass miR398-mediated gene down-regulation under drought stress. MicroRNA (miRNA) binding sites (MBSs) are frequently interrupted by introns and therefore require proper splicing to generate functional MBSs in target transcripts. MBSs can also be excluded during splicing of pre-messenger RNA, leading to different regulation among isoforms. Previous studies have shown that levels of Cu/Zn superoxide dismutase (CSD) are down-regulated by miR398...
August 3, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28771197/the-susceptibilities-of-respiratory-syncytial-virus-to-nucleolin-receptor-blocking-and-antibody-neutralization-are-dependent-upon-the-method-of-virus-purification
#7
Leanne M Bilawchuk, Cameron D Griffiths, Lionel D Jensen, Farah Elawar, David J Marchant
Respiratory Syncytial Virus (RSV) that is propagated in cell culture is purified from cellular contaminants that can confound experimental results. A number of different purification methods have been described, including methods that utilize fast protein liquid chromatography (FPLC) and gradient ultracentrifugation. Thus, the constituents and experimental responses of RSV stocks purified by ultracentrifugation in sucrose and by FPLC were analyzed and compared by infectivity assay, Coomassie stain, Western blot, mass spectrometry, immuno-transmission electron microscopy (TEM), and ImageStream flow cytometry...
August 3, 2017: Viruses
https://www.readbyqxmd.com/read/28752900/aberrant-astrocytic-expression-of-chondroitin-sulfate-proteoglycan-receptors-in-a-rat-model-of-amyotrophic-lateral-sclerosis
#8
Tomomi Shijo, Hitoshi Warita, Naoki Suzuki, Yasuo Kitajima, Kensuke Ikeda, Tetsuya Akiyama, Hiroya Ono, Shio Mitsuzawa, Ayumi Nishiyama, Rumiko Izumi, Masashi Aoki
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease. Progressive and systemic loss of motor neurons with gliosis in the central nervous system (CNS) is a neuropathological hallmark of ALS. Chondroitin sulfate proteoglycans (CSPGs) are the major components of the extracellular matrix of the mammalian CNS, and they inhibit axonal regeneration physically by participating to form the glial scar. Recently, protein tyrosine phosphatase sigma (PTPσ) and leukocyte common antigen-related protein were discovered as CSPG receptors that play roles in inhibiting regeneration...
July 28, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28747201/intravenous-superoxide-dismutase-as-a-protective-agent-to-prevent-impairment-of-lung-function-induced-by-high-tidal-volume-ventilation
#9
Nan-Chun Wu, Fan-Ting Liao, Hao-Min Cheng, Shih-Hsien Sung, Yu-Chun Yang, Jiun-Jr Wang
BACKGROUND: Positive-pressure mechanical ventilation is essential in assisting patients with respiratory failure in the intensive care unit and facilitating oxygenation in the operating room. However, it was also recognized as a primary factor leading to hospital-acquired pulmonary dysfunction, in which pulmonary oxidative stress and lung inflammation had been known to play important roles. Cu/Zn superoxide dismutase (SOD) is an important antioxidant, and possesses anti-inflammatory capacity...
July 26, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28745365/elemental-bio-imaging-of-pegylated-nayf4-yb-tm-gd-upconversion-nanoparticles-in-mice-by-laser-ablation-inductively-coupled-plasma-mass-spectrometry-to-study-toxic-side-effects-on-the-spleen-liver-and-kidneys
#10
Qing Li, Zheng Wang, Yirui Chen, Guoxia Zhang
Rare-earth upconversion nanoparticles (UCNPs) are considered stable nanoprobes with low toxicity and deep tissue penetration. However, the increasing use of UCNPs has raised concerns about their potential toxicity in living organisms. Very few studies have reported the toxicity of UCNPs; hence, it is not possible to conclude yet that UCNPs are safe. In this study, the distribution of PEGylated NaYF4:Yb/Tm/Gd nanoparticles (PEG-UCNPs) in female Balb/c mice following intravenous administration, and imaging in the spleen, liver and kidney was examined by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)...
August 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28728048/accumulation-and-detoxification-dynamics-of-chromium-and-antioxidant-responses-in-juvenile-rare-minnow-gobiocypris-rarus
#11
Cong Yuan, Meng Li, Yao Zheng, Ying Zhou, Feili Wu, Zaizhao Wang
Hexavalent chromium (Cr(6+)) compounds are hazardous via all exposure routes. To explore the dynamics of Cr accumulation and elimination and to reveal the mechanisms underlying detoxification and antioxidation in juvenile Gobiocypris rarus, one-month old G. rarus larvae were exposed to 0.1mgL(-1) Cr(6+) for four weeks for accumulation and subsequently placed to clean water for another week for depuration. The contents of Cr were measured weekly in the whole body of G. rarus juveniles. The activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione S-transferase (GST) and glutathione reductase (GR), and contents of glutathione (GSH) and malonaldehyde (MDA), and transcripts of cat, Cu/Zn-sod, Mn-sod, gpx1, gstpi, gr, mt1, nrf2 and uba52 were determined...
September 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28727077/excess-of-dietary-montmorillonite-impairs-growth-performance-liver-function-and-antioxidant-capacity-in-starter-pigs
#12
H Y Zhao, X B Mao, B Yu, J He, P Zheng, J Yu, J Q Luo, Q Y Wang, D W Chen
Montmorillonite (MMT) is widely used as a mycotoxin adsorbent in animal feeds, but its safety remains unclear. This study was conducted to investigate the safety of MMT supplementation in diets fed to starter pigs. A total of 120 32-d-old piglets (initial weight, 8.0 ± 0.9 kg) were randomly allotted into dietary treatments with graded MMT levels (0 [FS 0], 0.5% [FS 0.5], 1.0% [FS 1.0], 2.5% [FS 2.5], and 5.0% [FS 5.0]) with 6 replicate pens per treatment and 4 pigs per pen. All diets were fed for 28 d. As the MMT level increased, ADG and G:F changed in a linear and quadratic manner, while ADFI was linearly decreased ( > 0...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28720545/identification-and-kinetic-characterization-of-a-novel-superoxide-dismutase-from-avicennia-marina-an-antioxidant-enzyme-that-functions-under-a-broad-ph-and-temperature-conditions
#13
Farrokhzad Zeinali, Ahmad Homaei, Ehsan Kamrani
A novel Cu/Zn-superoxide dismutase was extracted from Avicennia marina and purified. The sample was collected from Khamir port located in the north shore of Persian Gulf. The purification procedure comprised of (NH4)2SO4 precipitation followed by CM-Sephadex C-50 and DEAE-Sepharose chromatography, and gel filtration chromatography (Sephadex G-75). The enzyme with a characteristic molecular weight of 31kDa, measured by SDS-page, showed its highest catalytic efficiency at pH 8.0 and 50°C. Its activity was greatly inhibited by cyanide and hydrogen peroxide...
July 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28704659/preheating-mitigates-cadmium-toxicity-in-zebrafish-livers-evidence-from-promoter-demethylation-gene-transcription-to-biochemical-levels
#14
Jia-Lang Zheng, Sai-Nan Guo, Shuang-Shuang Yuan, Hu Xia, Qing-Ling Zhu, Zhen-Ming Lv
The working hypothesis for this study was that moderate heat stress would alleviate the deleterious effects of subsequent cadmium (Cd) exposure on fish. Thus, zebrafish (Danio rerio) were subjected to water maintained at 26°C and 34°C for 4days, and then exposed to 0 or 200μg/L Cd for 1 week at 26°C. Multiple indicators were measured from livers of zebrafish at different levels, including DNA, RNA, protein and enzymatic activity associated with oxidative stress, inflammation and metal transport. The ameliorative effect of preheatinging on Cd toxicity was demonstrated...
September 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28686889/serpentine-endophytic-bacterium-pseudomonas-azotoformans-ass1-accelerates-phytoremediation-of-soil-metals-under-drought-stress
#15
Ying Ma, Mani Rajkumar, António Moreno, Chang Zhang, Helena Freitas
This study evaluates the potential of serpentine endophytic bacterium to foster phytoremediation efficiency of Trifolium arvense grown on multi-metal (Cu, Zn and Ni) contaminated soils under drought stress. A drought resistant endophytic bacterial strain ASS1 isolated from the leaves of Alyssum serpyllifolium grown in serpentine soils was identified as Pseudomonas azotoformans based on biochemical tests and partial 16S rRNA gene sequencing. P. azotoformans ASS1 possessed abiotic stress resistance (heavy metals, drought, salinity, antibiotics and extreme temperature) and plant growth promoting (PGP) properties (phosphate solubilization, nitrogen fixation, production of 1-aminocyclopropane-1-carboxylate deaminase, siderophore and ammonia)...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28686842/qm-mm-calculation-of-the-enzyme-catalytic-cycle-mechanism-for-copper-and-zinc-containing-superoxide-dismutase
#16
Masami Lintuluoto, Chiaki Yamada, Juha M Lintuluoto
The entire enzyme catalytic mechanism including the electron and the proton transfers of the copper- and zinc-containing extracellular superoxide dismutase (SOD3) was investigated by using QM/MM method. In the first step, the electron transfer from O2(·-) to SOD3 occurred without the bond formation between the donor and the acceptor and formed the triplet oxygen molecule and reduced SOD3. In the reduced SOD3, the distorted tetrahedral structure of Cu(I) atom was maintained. The reduction of Cu(II) atom induced the protonation of His113, which bridges between the Cu(II) and Zn(II) atoms in the resting state...
July 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28686251/oxygen-dependent-activation-of-cu-zn-superoxide-dismutase-1
#17
REVIEW
Morgan M Fetherolf, Stefanie D Boyd, Duane D Winkler, Dennis R Winge
Copper zinc superoxide dismutase (Sod1) is a critical enzyme in limiting reactive oxygen species in both the cytosol and the mitochondrial intermembrane space. Sod1 dismutes superoxide anions to hydrogen peroxide and oxygen. The catalytic reaction is dependent on an active site copper ion and a disulfide bonded conformation. The activation of Sod1 is mediated by its chaperone Ccs1. The mechanism of Ccs1-mediated Sod1 activation involves both insertion of the catalytic copper ion and mediating disulfide bond formation...
August 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28659953/overexpressing-the-sedum-alfredii-cu-zn-superoxide-dismutase-increased-resistance-to-oxidative-stress-in-transgenic-arabidopsis
#18
Zhen Li, Xiaojiao Han, Xixi Song, Yunxing Zhang, Jing Jiang, Qiang Han, Mingying Liu, Guirong Qiao, Renying Zhuo
Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)-scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28659943/winter-night-warming-improves-post-anthesis-physiological-activities-and-sink-strength-in-relation-to-grain-filling-in-winter-wheat-triticum-aestivum-l
#19
Yonghui Fan, Zhongwei Tian, Yanyan Yan, Chenxi Hu, Muhammad Abid, Dong Jiang, Chuanxi Ma, Zhenglai Huang, Tingbo Dai
The diurnal and seasonal temperature rising patterns "asymmetric warming," plays an important role in crop distribution and productivity. Asymmetric warming during the early growth periods of winter wheat (Triticum aestivum L.) profoundly affects vegetative growth and post-anthesis grain productivity, but the underlying physiological mechanism is still unclear. We conducted field experiments from 2012 to 2014 using two wheat cultivars, namely, Yangmai-13 (vernal type) and Yannong-19 (semi-winter type), to investigate the influences of night-warming during the winter (warming by 1...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28649040/oxidative-potential-of-particulate-matter-2-5-as-predictive-indicator-of-cellular-stress
#20
Bélinda Crobeddu, Leticia Aragao-Santiago, Linh-Chi Bui, Sonja Boland, Armelle Baeza Squiban
Particulate air pollution being recognized to be responsible for short and long term health effects, regulations for particulate matter with an aerodynamic diameter less than 2.5 (PM2.5) are more and more restrictive. PM2.5 regulation is based on mass without taking into account PM2.5 composition that drives toxicity. Measurement of the oxidative potential (OP) of PM could be an additional PM indicator that would encompass the PM components involved in oxidative stress, the main mechanism of PM toxicity. We compared different methods to evaluate the intrinsic oxidative potential of PM2...
June 22, 2017: Environmental Pollution
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