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H Trimarchi, R Canzonieri, A Schiel, C Costales-Collaguazo, J Politei, A Stern, M Paulero, T Rengel, J Andrews, M Forrester, M Lombi, V Pomeranz, R Iriarte, A Muryan, E Zotta, M D Sanchez-Niño, A Ortiz
BACKGROUND: Certain glomerulopathies are associated with increased levels of CD80 (B7-1). We measured the urinary excretion of CD80, podocyturia and proteinuria in controls and in subjects with Fabry disease either untreated or on enzyme replacement therapy (ERT). METHODS: Cross-sectional study including 65 individuals: controls (n = 20) and Fabry patients (n = 45, 23 of them not on ERT and 22 on ERT). Variables included age, gender, urinary protein/creatinine ratio (UPCR), estimated glomerular filtration rate (eGFR), urinary uCD80/creatinine ratio (uCD80) and podocyturia...
October 13, 2016: Journal of Translational Medicine
Tianyue Zhai, Itsuko Furuta, Rina Akaishi, Satoshi Ishikawa, Mamoru Morikawa, Takahiro Yamada, Takahiro Koyama, Hisanori Minakami
Podocyte injury has been suggested to induce phenotypic alteration of glomerular podocytes and accelerate the detachment of podocytes from the glomeruli resulting in podocyturia. However, it is not clear whether podocyte phenotypic alteration occurs in the urine of women with preeclampsia (PE). Seventy-seven and 116 pelleted urine samples from 38 and 18 women at various stages of normal and PE pregnancies, respectively underwent quantitative analysis of podocyte-specific or associated protein mRNA expression, including podocin, nephrin, and synaptopodin using RT-PCR...
2016: Scientific Reports
Ester Miranda Pereira, Adalberto Socorro da Silva, Anatália Labilloy, José Tiburcio do Monte Neto, Semiramis Jamil Hadad do Monte
INTRODUCTION: Fabry disease is a lysosomal storage disorder due to abnormalities in the GLA gene (Xq22). Such changes result in the reduction/absence of activity of the lysosome enzyme α-GAL, whose function is to metabolize globotriaosylceramide (Gb3). Renal disease is a major clinical outcome of the accumulation of Gb3. Podocyte injury is thought to be a major contributor to the progressive loss of the renal function and may be found altered even before the onset of microalbuminuria...
March 2016: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
Hernán Trimarchi, Romina Canzonieri, Amalia Schiel, Juan Politei, Aníbal Stern, José Andrews, Matías Paulero, Tatiana Rengel, Alicia Aráoz, Mariano Forrester, Fernando Lombi, Vanesa Pomeranz, Romina Iriarte, Pablo Young, Alexis Muryan, Elsa Zotta
No abstract text is available yet for this article.
March 25, 2016: Journal of Nephrology
H Trimarchi, R Canzonieri, A Muryan, A Schiel, A Araoz, M Paulero, J Andrews, T Rengel, M Forrester, F Lombi, V Pomeranz, R Iriarte, E Zotta
No specific or efficient treatment exists for Alport syndrome, an X-linked hereditary disease caused by mutations in collagen type IV, a crucial component of the glomerular basement membrane. Kidney failure is usually a major complication of the disease, and patients require renal replacement therapy early in life. Microhematuria and subsequently proteinuria are hallmarks of kidney involvement, which are due to primary basement membrane alterations that mainly cause endothelial thrombosis and podocyte contraction and ulterior irreversible detachment...
2016: Case Reports in Nephrology
Juliana B Mansur, Amélia R P Sabino, Sonia K Nishida, Gianna Mastroianni Kirsztajn
OBJECTIVE: To establish the occurrence and intensity of podocyturia and its relation to grade of disease activity, as defined by clinical and laboratory criteria. METHODS: Prospective, cross-sectional study involving 50 patients with lupus nephritis and 29 controls, which had podocyturia levels determined from random urine samples using an immunofluorescence technique. Disease activity was graded by BILAG (renal criteria) and an additional system used in the service (S2)...
2016: Renal Failure
Hernán Trimarchi, Romina Canzonieri, Amalia Schiel, Juan Politei, Aníbal Stern, José Andrews, Matías Paulero, Tatiana Rengel, Alicia Aráoz, Mariano Forrester, Fernando Lombi, Vanesa Pomeranz, Romina Iriarte, Pablo Young, Alexis Muryan, Elsa Zotta
BACKGROUND: Proteinuria suggests kidney involvement in Fabry disease. We assessed podocyturia, an early biomarker, in controls and patients with and without enzyme therapy, correlating podocyturia with proteinuria and renal function. METHODS: Cross-sectional study (n = 67): controls (Group 1, n = 30) vs. Fabry disease (Group 2, n = 37) subdivided into untreated (2A, n = 19) and treated (2B, n = 18). Variables evaluated: age, gender, creatinine, CKD-EPI, proteinuria, podocyte count/10 20× microscopy power fields, podocytes/100 ml urine, podocytes/g creatininuria (results expressed as median and range)...
February 3, 2016: Journal of Nephrology
Elisa Rossi, David M Smadja, Elisa Boscolo, Carmen Langa, Miguel A Arevalo, Miguel Pericacho, Luis Gamella-Pozuelo, Alexandre Kauskot, Luisa M Botella, Pascale Gaussem, Joyce Bischoff, José M Lopez-Novoa, Carmelo Bernabeu
The circulatory system is walled off by different cell types, including vascular mural cells and podocytes. The interaction and interplay between endothelial cells (ECs) and mural cells, such as vascular smooth muscle cells or pericytes, play a pivotal role in vascular biology. Endoglin is an RGD-containing counter-receptor for β1 integrins and is highly expressed by ECs during angiogenesis. We find that the adhesion between vascular ECs and mural cells is enhanced by integrin activators and inhibited upon suppression of membrane endoglin or β1-integrin, as well as by addition of soluble endoglin (SolEng), anti-integrin α5β1 antibody or an RGD peptide...
April 2016: Cellular and Molecular Life Sciences: CMLS
N V Chebotareva, I N Bobkova, N V Neprintseva, L V Kozlovskaia, Z T Malkandueva
AIM: To estimate the degree of podocyte injury in patients with different types of chronic glomerulonephritis (CGN) from the urinary level of podocyte markers and to determine the significance of these indicators as criteria for disease activity and prognosis. SUBJECTS AND METHODS: Seventy-three patients with CGN, including 20 with inactive nephritis (Group 1), 23 with obvious urological syndrome (Group 2), 30 with nephrotic syndrome (NS) (Group 3), among them there were 7 patients with severe NS and 7 with NS concurrent with acute nephritic syndrome, were examined...
2015: Terapevticheskiĭ Arkhiv
Sane Vianna Pereira, Mariane Dos Santos, Patrícia Garcia Rodrigues, Jonathan Fraportti do Nascimento, João Rodolfo Teló Timm, Rafael Zancan, Rogério Friedman, Francisco Veríssimo Veronese
OBJECTIVE: The aim of this study was to correlate different degrees of excess weight with the expression of podocyte-associated messenger RNAs (mRNAs) in urine. METHODS: The sample comprised 83 patients with overweight or obesity class I, II, or III and 18 healthy controls. The expression of nephrin, podocin, podocalyxin, α-actinin-4, α3β1integrin, vascular endothelial growth factor, and transforming growth factor-beta (TGF-β1 ) mRNA in urine was quantified with the real-time polymerase chain reaction...
August 2015: Obesity
H Trimarchi, R Canzonieri, A Muryan, A Schiel, A Araoz, M Forrester, A Karl, F Lombi, J Andrews, V Pomeranz, T Rengel, E Zotta
The time for starting a patient with Fabry disease on enzyme replacement therapy is still a matter of debate, particularly when no overt classical clinical signs or symptoms are present. With respect to Fabry nephropathy, a dual problem coexists: the reluctance of many nephrologists to start enzyme replacement infusion until signs of renal disease appear as the appearance of proteinuria or an elevation in serum creatinine and the lack of validated biomarkers of early renal damage. In this regard, proteinuria is nowadays considered as an early and appropriate marker of kidney disease and of cardiovascular morbidity and mortality...
2015: Case Reports in Nephrology
Fernanda Badiani Roberto, Thaís Alquezar Facca, Jussara Leiko Sato, Amélia Rodrigues Pereira Sabino, Sonia Kiyomi Nishida, Gianna Mastroianni-Kirsztajn, Nelson Sass
PURPOSE: To evaluate the presence of podocyturia in chronic hypertensive pregnant women in the third trimester of pregnancy and its possible association with renal disease. METHODS: This was an observational study of a convenience sample of 38 chronic hypertensive pregnant women. The podocytes were labeled by the indirect immunofluorescence technique with anti-podocin and diamidino-phenylindole (DAPI). The count was made on 30 random fields analyzed and corrected according to urinary creatinine (podocytes/mg creatinine)...
April 2015: Revista Brasileira de Ginecologia e Obstetrícia
Hernan Trimarchi
Glomerulopathy is the third most important cause of kidney disease. Proteinuria is the hallmark of glomerular damage, and a marker of progression of kidney disease, cardiovascular morbidity and mortality. Strategies to reduce proteinuria are partially successful, and despite proteinuria management, renal disease may still progress. Immunosuppression to treat glomerulopathies is nonspecific, partially effective and presents side-effects. It is critical to find safe drugs with specific podocyte molecular targets...
2015: Recent Patents on Endocrine, Metabolic & Immune Drug Discovery
Turgay Saritas, Marcus J Moeller
No abstract text is available yet for this article.
November 2014: Nature Reviews. Nephrology
Ylbe Palacios de Franco, Karina Velazquez, Natalia Segovia, Carolina Acosta, Deborah Yanosky, Ylbe V Franco Palacios, Amanda Ramos, Carlos R Franco Palacios
BACKGROUND: Preeclampsia is associated with significant materno-fetal morbidity and mortality. Podocyturia due to podocyte damage seems to be associated with the disease. The objective of this study was to evaluate the usefulness of urinary podocalyxin as a marker of preeclampsia in a Hispanic population. METHODS: 63 patients were studied. 25 patients had preeclampsia/eclampsia (PE-E). 38 patients had normal pregnancies and served as control group. 24 hour proteinuria, urine protein/creatinine (UPC), urinary podocalyxin and perinatal outcomes were measured...
2014: International Journal of Physiology, Pathophysiology and Pharmacology
Belinda Jim, Swati Mehta, Andi Qipo, Kwanghee Kim, Hillel W Cohen, Robert M Moore, John C He, Shuchita Sharma
Preeclampsia, a hypertensive multisystem disease that complicates 5-8% of all pregnancy, is a major cause for maternal and fetal mortality and morbidity. The disease is associated with increased spontaneous and evoked preterm birth and remote cardio-renal disorders in the mother and offspring. Thus the ability to predict the disease should lead to earlier care and decreased morbidity. This has led to fervent attempts to identify early predictive biomarkers and research endeavors that have expanded as we learn more regarding possible causes of the disease...
2014: PloS One
Patrícia Garcia Rodrigues, Rafael Nazário Bringhenti, Jonathan Frapporti do Nascimento, Gabriel Joelsons, Mariane dos Santos, Sane Pereira, Francisco Veríssimo Veronese
AIM: It is not clear how the podocyte damage manifests in different glomerulopathies. This study evaluated the podocyte-associated mRNA profiles in renal tissue and urine of patients with proliferative (PGs) or non-proliferative (NPGs) glomerulopathies. METHODS: Messenger RNA levels of nephrin, podocin, podocalyxin, synaptopodin, and alpha-actinin-4 were measured in the kidney tissue and urinary cells by real-time polymerase chain reaction. Podocyte-associated mRNAs were correlated with proteinuria and renal function, and the effect of immunosuppressive treatment of PGs and NPGs on urine mRNAs was assessed up to one year of follow up...
2014: International Journal of Clinical and Experimental Pathology
Suzanne R Hayman, Juan C Calle, Aminah Jatoi, Iasmina M Craici, Steven J Wagner, Amy L Weaver, Eddie L Greene, Joseph P Grande, Vesna D Garovic
BACKGROUND: Urinary podocyte excretion (podocyturia) may function as a more specific marker of ongoing glomerular damage. This study sought to analyze the relationship between proteinuria and podocyturia in cancer patients treated with antivascular endothelial growth factor (anti-VEGF) agents. METHODS: Thirty-seven patients treated with anti-VEGF medications were analyzed in a single-institution, cross-sectional study. Podocyte cultures were performed on random urine collections (50-100 ml), and podocytes were identified by positive podocin staining...
2014: Oncology
Wendy M White, Angelica T Garrett, Iasmina M Craici, Steven J Wagner, Patrick D Fitz-Gibbon, Kim A Butters, Brian C Brost, Carl H Rose, Joseph P Grande, Vesna D Garovic
OBJECTIVE: Studies have shown that podocyturia, i.e., urinary loss of viable podocytes (glomerular epithelial cells), is associated with proteinuria in preeclampsia. We postulated that urinary podocyte loss may persist after preeclamptic pregnancies, thus resulting in renal injury. This may lead to future chronic renal injury. In addition, we compared the postpartum levels of the angiogenic factors, which previously have been associated with preeclampsia, between normotensive versus preeclamptic pregnancies...
2014: PloS One
Amelia Rodrigues Pereira Sabino, Vicente de Paulo Castro Teixeira, Sonia Kiyomi Nishida, Nelson Sass, Juliana Busato Mansur, Gianna Mastroianni Kirsztajn
INTRODUCTION: The podocyturia has been detected in glomerular diseases, such as lupus nephritis (LN), in which proteinuria is an important manifestation, and its occurrence seems to be limited to the active phase of the disease. OBJECTIVE: To evaluate podocyturia in LN patients, and the possible association with clinical disease activity. METHODS: We evaluated 56 patients with LN, that were classified in three groups according to the degree of clinical activity: Group B, no activity (n = 17), Group C with mild (n = 29) and Group D, moderate to severe activity (n = 10)...
October 2013: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
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