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https://www.readbyqxmd.com/read/28582601/hematologic-ris-for-healthy-water-buffaloes-bubalus-bubalis-in-southern-mexico
#1
Oswaldo M Torres-Chable, Nadia F Ojeda-Robertos, Alfonso J Chay-Canul, Jorge A Peralta-Torres, Carlos Luna-Palomera, Nahum Brindis-Vazquez, Bradley J Blitvich, Carlos Machain-Williams, Julian E Garcia-Rejon, Carlos M Baak-Baak, Karin S Dorman, Miguel A Alegria-Lopez
BACKGROUND: Baseline hematologic data are used by veterinarians and wildlife biologists to perform health assessments on target animal species. Hematologic measurements are influenced by various factors including geography. Baseline hematologic RIs have been established for domestic water buffaloes (Bubalus bubalis) from various countries in the Eastern Hemisphere, but these data are not readily available for the Western Hemisphere. OBJECTIVE: The aim of this study was to determine hematologic values for domestic water buffaloes from several commercial farms in southern Mexico...
June 5, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28543808/comparison-of-two-methods-of-seminal-plasma-removal-on-buffalo-bubalus-bubalis-sperm-cryopreservation
#2
R S Albuquerque, R Morais, A N Reis, M S Miranda, Ear Dias, F M Monteiro, Ccp Paz, M Nichi, Gkv Kawai, Cpf Della'Aqua, F O Papa, R B Viana, L U Gimenes
Cryopreservation causes damage to spermatozoa, and methods minimizing this damage are therefore needed. Although much discussed, seminal plasma removal has become an alternative to improve sperm quality and viability after freezing and has been applied to different species in attempt to obtain good results. The objective of this study was to evaluate semen quality in buffaloes submitted to two methods for seminal plasma removal (filtration and centrifugation). Semen samples were collected from seven Murrah buffalo bulls (Bubalus bubalis) once a week for 8 weeks...
May 19, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28488738/comparison-of-three-different-extenders-on-murrah-buffaloes-bubalus-bubalis-semen-freezability
#3
M F Zorzetto, I Martin, Y F R Sancler-Silva, S Zoca, C P Freitas-Dell'Aqua, F O Papa, A A Ramos, J F Nunes, C C M Salgueiro, E Oba
The use of frozen semen for artificial insemination is the main approach utilised for the genetic improvement of most domesticated species. The advantages include lower transportation costs, continuous availability of semen, fewer occurrences of sexually transmitted diseases and the incorporation of desirable genes in a relatively short amount of time. Nevertheless, the use of frozen semen in buffalo herds remains limited due to the loss of sperm quality when buffalo semen is frozen. So, the goal of this study was to evaluate the pre- and post-cryopreservation quality of buffalo semen diluted in three distinct freezing media: Tris-egg yolk, Botu-bov® (BB) and ACP-111®...
May 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28460685/ovulatory-and-fertility-response-using-modified-heatsynch-and-ovsynch-protocols-in-the-anovular-murrah-buffalo-bubalus-bubalis
#4
K Karuppanasamy, R K Sharma, S K Phulia, A Jerome, K M Kavya, S P S Ghuman, H Kumar, I Singh, N Krishnaswamy
We studied the effect of modified Heatsynch and Ovsynch protocols on the ovulatory response (OR), estrus induction rate (EIR) and conception rate (CR) in the anovular postpartum Murrah buffalo (n = 35). In the modified Heatsynch protocol (Group I; n = 12), buffaloes were given two GnRH at 2 h interval on treatment day 0, PGF (PGF2α) on day 7 and estradiol (E2) 1 mg on day 8. Two FTAI were done at 20 h intervals after E2 administration. In the modified Ovsynch protocol (Group II; n = 15), GnRH was given on day 0, 7 and 16 with a PGF on day 14...
June 2017: Theriogenology
https://www.readbyqxmd.com/read/28452126/plasma-proteome-of-buffaloes
#5
Leticia Gomes de Pontes, Nayara Rodrigues Vieira Cavassan, Luciana Curtolo de Barros, Rui Seabra Ferreira Junior, Benedito Barraviera, Lucilene Delazari Dos Santos
The proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. Water buffaloes have gained prominence in the world economy due to the quality of their milk, meat and leather, in addition to being an important donor of blood components. This work aimed to identify and characterize the proteins present in the blood plasma of Murrah buffaloes (Bubalus bubalis) through 2D-electrophoresis, in gel protein digestion followed by mass spectrometry technique and for albumin depletion, in solution protein digestion followed by shotgun analysis...
April 28, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28412260/is-oral-biopsy-associated-with-change-in-tobacco-or-alcohol-use
#6
Tiffany M Peters, Ceib Phillips, Valerie A Murrah
PURPOSE: Tobacco and alcohol remain the predominant risk factors for oral cancer, but the relation between having an oral biopsy and cessation of these risk factors is unknown. Therefore, this investigation examined whether there might be an association between oral biopsy and change in risk factor use. MATERIALS AND METHODS: A survey was sent to a cohort consisting of a consecutive sample of subjects identified in the University of North Carolina Oral Pathology database...
March 22, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28344410/identification-of-single-nucleotide-polymorphism-in-protein-phosphatase-1-regulatory-subunit-11-gene-in-murrah-bulls
#7
Varsha Jain, Brijesh Patel, Farhat Paul Umar, H M Ajithakumar, Suraj K Gurjar, I D Gupta, Archana Verma
AIM: This study was conducted with the objective to identify single nucleotide polymorphism (SNP) in protein phosphatase 1 regulatory subunit 11 (PPP1R11) gene in Murrah bulls. MATERIALS AND METHODS: Genomic DNA was isolated by phenol-chloroform extraction method from the frozen semen samples of 65 Murrah bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal. The quality and concentration of DNA was checked by spectrophotometer reading and agarose gel electrophoresis...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28344404/a-combinatorial-model-for-effective-estrus-detection-in-murrah-buffalo
#8
Ramu Muthu Selvam, Govindaraju Archunan
BACKGROUND: Buffaloes are silent heat animals and lacunae in their estrus detection results a substantial economic loss in developing countries. Many advanced tools to aid heat detection have been developed but are neither affordable nor easily interpretable by marginal farmers. AIM: The present investigation was made to develop a cost-effective estrus detection model by combining several known estrus predicting parameters. MATERIALS AND METHODS: Various signs of estrus were classified under major parameters such as visual, cow behavioral, bull behavioral, biochemical, and gyneco-clinical...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28294426/polymorphism-in-5-untranslated-region-of-heat-shock-protein-70-gene-as-marker-of-post-partum-anoestrus-in-murrah-buffaloes
#9
R Kumar, M Ghosh, N Kumar, A K Balhara, M Gupta, R K Sharma, I Singh
The enormous production potential of buffaloes has never been accomplished due to various reproductive insufficiencies. Among them, post-partum anoestrus, a multifactorial disorder, is predominant but any genetic association is yet to be established. This study focused to identify novel polymorphisms in heat-shock protein 70 (HSP70) gene and its possible association with post-partum anoestrus in Murrah buffaloes. A 579-bp fragment from 5' untranslated region of HSP70 gene was amplified by polymerase chain reaction from blood genomic DNA of 614 animals maintained under similar management conditions...
June 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28262463/spermatozoa-with-high-mitochondrial-membrane-potential-and-low-tyrosine-phosphorylation-preferentially-bind-to-oviduct-explants-in-the-water-buffalo-bubalus-bubalis
#10
Kaustubh Kishor Saraf, Arumugam Kumaresan, Shivani Chhillar, Samiksha Nayak, Sreela Lathika, Tirtha Kumar Datta, Subhash Chand Gahlot, Prabha Karan, Kiran Verma, Tushar Kumar Mohanty
Although it is understood that spermatozoa are subjected to selection processes to form a functional sperm reservoir in the oviduct, the mechanism remains obscure. With the aim to understand the sperm selection process in the oviduct, in the present in vitro study, we analyzed mitochondrial membrane potential and tyrosine phosphorylation status in oviduct-explants bound and unbound spermatozoa. Frozen semen from Murrah buffalo bulls (n=10) used under progeny testing programme were utilized for the study. Oviduct explants were prepared by overnight culture of epithelial cells in TCM- 199 and washed spermatozoa were added to the oviduct explants and incubated for 4h...
February 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28246443/study-of-the-dairy-characters-of-lactating-murrah-buffaloes-on-the-basis-of-body-parts-measurements
#11
Sandeep Dhillod, Dipankar Kar, C S Patil, Subhasish Sahu, Narender Singh
AIM: The aim of the study was to correlate the milk yield of Murrah buffaloes with certain body parts measurements. MATERIALS AND METHODS: A total of 70 lactating Murrah buffaloes were selected from Buffalo Farm, Lala Lajpat Rai University of Veterinary and Animal Science, Hisar and were randomly selected in a range from first to fifth parity. Traits studied were 305 days milk yield (MY), body weight (BW), body length (BL), muzzle width (MW), height at wither (HW), abdominal girth (AG), chest girth (CG), body depth fore, body depth rear, hip bone distance (HBD), pin bone distance (PBD), skin thickness (STK), and tail length (TL)...
January 2017: Veterinary World
https://www.readbyqxmd.com/read/28242954/breed-traceability-of-buffalo-meat-using-microsatellite-genotyping-technique
#12
Bheemashankar H Kannur, Md Nadeem Fairoze, P S Girish, Nagappa Karabasanavar, B H Rudresh
Although buffalo has emerged as a major meat producing animal in Asia, major research on breed traceability has so far been focused on cattle (beef). This research gap on buffalo breed traceability has impelled development and validation of buffalo breed traceability using a set of eight microsatellite (STR) markers in seven Indian buffalo breeds (Bhadawari, Jaffaarabadi, Murrah, Mehsana, Nagpuri, Pandharpuri and Surti). Probability of sharing same profile by two individuals at a specific locus was computed considering different STR numbers, allele pooling in breed and population...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28207851/impact-of-levels-of-total-digestible-nutrients-on-microbiome-enzyme-profile-and-degradation-of-feeds-in-buffalo-rumen
#13
Anju Kala, D N Kamra, Avinash Kumar, Neeta Agarwal, L C Chaudhary, C G Joshi
The present study was aimed at understanding a shift in rumen microbiome of buffaloes fed various levels of total digestible nutrients. To understand the process, the metagenomics of rumen microbes, in vivo and in vitro rumen fermentation studies were carried out. Three rumen fistulated adult male Murrah buffaloes were fed three isonitrogenous diets varying in total digestible nutrients (70, 85 and 100% of TDN requirement) in 3X3 switch over design. On dry matter basis, wheat straw/ roughage content were 81, 63 and 51% and that of maize grain was 8, 16 and 21% in three diets respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28176851/comparative-study-of-the-gut-microbiome-potentially-related-to-milk-protein-in-murrah-buffaloes-bubalus-bubalis-and-chinese-holstein-cattle
#14
Jiachao Zhang, Chuanbiao Xu, Dongxue Huo, Qisong Hu, Qiannan Peng
Previous studies suggested a close relationship between ruminant gut microbes and the mammary gland. In this study, shotgun metagenomic sequencing was used to reveal the differences in the intestinal microbiome potentially related to milk components in Murrah buffaloes and Chinese Holstein cattle. A PCoA based on the weighted Unifrac distances showed an apparent clustering pattern in the structure of intestinal microbiota between buffalo and cattle. We could attribute the structural difference to the genera of Sutterella, Coprococcus and Dorea...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28176388/urine-levels-of-luteinizing-hormone-as-predictor-of-the-period-of-ovulation-for-advantage-of-timed-artificial-insemination-in-murrah-buffalo-bubalus-bubalis
#15
R M Selvam, D Singh, M A Akbarsha, G Archunan
Assessment of urine levels of luteinizing hormone (LH) for predicting the reproductive status of animals is in practice. The aim of this study was to predict the period of ovulation based on the urine levels of LH for timed-artificial insemination to increase the conception rate in buffaloes, which are naturally silent-oestrous animals. Level of LH in urine was assessed using ELISA, and a cut-off LH concentration for prediction of ovulation period was obtained using receiver operating characteristic analysis...
June 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28096623/estrus-induction-and-fertility-response-following-different-treatment-protocols-in-murrah-buffaloes-under-field-conditions
#16
L Kumar, J B Phogat, A K Pandey, S K Phulia, S Kumar, J Dalal
AIM: The aim of this study was to evaluate the efficacy of three different treatment protocols for estrus induction and conception rate in postpartum anestrus buffaloes during breeding season under field conditions. MATERIALS AND METHODS: The 47 postpartum anestrus buffaloes of the 2(nd) to 6(th) parity were divided into three groups. Group 1 (n=16): Buffaloes received cosynch treatment, that is, buserelin acetate 10 µg on day 0 and 9, cloprostenol 500 µg on day 7 followed by fixed-time artificial insemination (FTAI) at the time of second buserelin acetate and 24 h later...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28026086/identification-of-serum-protein-markers-for-early-diagnosis-of-pregnancy-in-buffalo
#17
Lukumoni Buragohain, Trilok Nanda, Arnab Ghosh, Mayukh Ghosh, Rajesh Kumar, Sunil Kumar, Sambhu Sharan Gupta, Arpita Bharali, Ashok K Mohanty, Inderjeet Singh, Ashok Kumar Balhara
Improper or delayed pregnancy diagnosis has significant impact over animal production, particularly in buffaloes which inherently suffer from several reproductive inefficiencies. Thus the present study has undertaken to identify serum protein markers pertaining to early pregnancy diagnosis in buffaloes. Serum samples were collected from 10 pregnant Murrah Buffalo heifers at weekly intervals from days 0-35 post-artificial insemination and from 12 inseminated non-pregnant cyclic buffalo heifers on days 0, 7, 14 and 21...
December 27, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28011259/exploration-of-luteinizing-hormone-in-murrah-buffalo-bubalus-bubalis-urine-extended-surge-window-opens-door-for-estrus-prediction
#18
Ramu Muthu Selvam, Suneel Kumar Onteru, Varij Nayan, Muthusamy Sivakumar, Dheer Singh, Govindaraju Archunan
Estrus detection in buffaloes has been a major concern for decades, and lack of reliable methods affects their effective reproductive management. Luteinizing hormone (LH) detection in urine is in practice for several mammals for timed insemination, whereas very few reports are available on buffalo urinary LH. The focus of this study is to detect the presence of LH in buffalo urine, quantitate variation in urinary LH during different estrous cycle phases and examine the duration of mid-cycle LH window. Nearly hundred buffaloes were examined, longitudinal urine samples (n=42) were collected from seventeen animals and classified into respective phases based on several estrus detection parameters...
December 21, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28004872/random-regression-models-to-estimate-genetic-parameters-for-weights-in-murrah-buffaloes
#19
Flavia Rita Ferreira, Francisco Ribeiro de Araujo Neto, Henrique Barbosa Borges, Rusbel Raul Aspilcueta-Borquis, Naudim Alejandro Hurtado-Lugo, Henrique Nunes de Oliveira, Lucia Galvão de Albuquerque, Humberto Tonhati
This article reports genetic analysis of the weight at different ages of Murrah water buffaloes, using random regression models (RRM). Models ranging from third to sixth order polynomial were used to describe direct genetic and animal permanent environmental effects. Contemporary group was included as a fixed effect, and a cubic polynomial was used to model the mean curve of the population. The residual was modeled considering a log-linear function. Two models were selected for study of genetic parameters. The first model included third and sixth order polynomials for direct genetic and animal permanent environmental effects (M36)...
December 22, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27847429/evaluation-of-salivary-electrolytes-during-estrous-cycle-in-murrah-buffaloes-with-reference-to-estrus-detection
#20
Indu Devi, Pawan Singh, Surerder Singh Lathwal, A Kumaresan, Kuldeep Dudi
AIM: Timely estrus detection is one of the critical factors for increasing reproductive efficiency in buffaloes. In recent decades, saliva has become a more popular as a noninvasive source for determining physiological status of animals by various biochemical electrolytes. This study was designed to assess and correlate changes in different salivary minerals concentration (calcium, inorganic phosphorus, magnesium, sodium, potassium, and chloride) during different stages of the estrous cycle in Murrah buffaloes...
October 2016: Veterinary World
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