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Sourabh Sulabh, Manjit Panigrahi, Sheikh Firdous Ahmad, Rajat Varshney, Ankita Verma, Naseer Ahmad Baba, Satish Kumar, Soni Kumari, Anuj Chauhan, Pushpendra Kumar, Bharat Bhushan
Subclinical mastitis, generally caused by Staphylococcus aureus, has a global economic impact all over the world. Hence, it needs to be resolved on higher priority which may be attained via. selection of mastitis resistant animals or inclusion of mastitis resistant trait into herd apart from management care. Diverse hosts with various genetic make-ups encounter pathogens in a diverse manner which in turn leads to contradicting outcome of the disease. Identification of species-wise or breed-wise differential expressed genes in response to S...
May 2, 2018: Animal Biotechnology
K K Choudhary, A Bharadwaj, R K Sharma, A Jerome, S Khanna, K M Kavya
This study was designed to investigate the sensitivity and specificity of three methods for detecting the onset of cyclicity in post-partum Murrah buffaloes. The methods investigated were visual signs, transrectal ultrasonography, and serum progesterone (P4 ) assay. For this study, 102 post-partum Murrah buffalo cows were grouped for monitoring their ovarian activity. The first group of buffaloes was between 26 and 35 days post-partum. Thereafter, the buffalo cows that calved were grouped after every 10 days for the study sample...
April 18, 2018: Tropical Animal Health and Production
Deshun Shi, Xiangping Li, Zhaocheng Xiong, Haihang Zhang, Ben Huang, Qingyou Liu, Yingqun Wang
Objective: The aim of the current study is to investigate the relationship between PHB, CAPZB, and TEKT2 and sperm motility in Murrah buffalo. Methods: We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB and TEKT2 in mRNA (message RNA) and protein level. Results: QRT-PCR (Quantitative Reverse Transcription-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB...
April 12, 2018: Asian-Australasian Journal of Animal Sciences
Kavita, Jagat Bir Phogat, Anand Kumar Pandey, Ashok Kumar Balhara, Sarvpreet Singh Ghuman, Pankaj Gunwant
Buffalo heifers have tendency to show anestrus during summer season. Melatonin has been used for correcting summer dependent anestrous via inducing resumption of ovarian activity. Therefore, the investigation was conducted to compare efficacy of melatonin for induction of estrus and conception rate with Ovsynch protocol in summer anestrous Murrah buffalo heifers. Thirty, summer anestrous Murrah buffalo heifers were selected and divided into two groups- treatment (n = 20; 12 melatonin implants) and control (n = 10; no treatment)...
March 27, 2018: Reproductive Biology
André M Santana, Daniela G Silva, Virna Clemente, Lucas J L Pizauro, Priscila A Bernardes, Clarissa H Santana, Peter D Eckersall, José J Fagliari
BACKGROUND: There is a lack of published work on RIs for newborn buffaloes. Establishing blood gas and serum biochemical RIs for newborn buffaloes is important for monitoring health. OBJECTIVES: This study establishes blood gas and serum biochemical RIs of newborn buffaloes. METHODS: Twenty-eight newborn buffaloes, 10-30 days old, were selected. Thirty blood biochemical variables were analyzed. The Anderson-Darling test was used to assess the normality of the distribution...
March 2018: Veterinary Clinical Pathology
Chetna Gangwar, Atul Saxena, Akhil Patel, S P Singh, Sarvajeet Yadav, Ravindra Kumar, Vijay Singh
The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done...
March 9, 2018: Animal Reproduction Science
A N Sharma, S Kumar, A K Tyagi
A study of 120 days was undertaken to ascertain the effect of mannan-oligosaccharides (MOS) and Lactobacillus acidophilus supplementation on growth performance, nutrient utilization and faecal characteristics in Murrah buffalo calves. Twenty Murrah buffalo calves of 5-7 days old and 31 ± 2.0 kg of body weight (BW) were randomly assigned into four groups. Group I served as the control (CON) in which only basal diet (concentrate mixture and green fodder) was provided, without any supplementation. Mannan-oligosaccharides at 4 g/calf/day were supplemented as prebiotic to Group II (PRE), whereas Group III (PRO) received Lactobacillus acidophilus in the form of fermented milk as probiotic at 200 ml/calf/day having 108  CFU/ml and Group IV (SYN) was supplemented with both MOS and Lactobacillus acidophilus as synbiotic at similar dose...
June 2018: Journal of Animal Physiology and Animal Nutrition
Andres Flores-Hidalgo, Si On Lim, Alice E Curran, Ricardo J Padilla, Valerie Murrah
OBJECTIVE: We report here the 10-year experience with oral hairy leukoplakia (OHL) at the Division of Oral and Maxillofacial Pathology at the University of North Carolina at Chapel Hill, NC, USA. STUDY DESIGN: All the associated hematoxylin and eosin and Epstein-Barr virus encoding region in situ hybridization slides of OHL cases between January 1, 2008, and February 1, 2017, were retrieved and reviewed. Collected demographic characteristics, clinical presentation, medical and social histories were reviewed and reported...
March 2018: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Puneeth Kumar, Tushar Kumar Mohanty, Arumugam Kumaresan, Pradeep Nag, Kaustubh Kishor Saraf, Vimlesh Kumar, Sreela Lathika, Samiksha Nayak, Mukesh Bhakat
Anandamide (AEA), an endocannabinoid, has been shown to reduce capacitation and acrosomal exocytosis in human spermatozoa. Because buffalo spermatozoa are highly susceptible to cryopreservation induced damage, AEA was assessed as to whether it could protect spermatozoa from cryo-damage. Six ejaculates from six Murrah buffalo bulls (total 36 ejaculates) were utilized for the study. Each ejaculate was divided into four aliquots; spermatozoa in Aliquot 1 were extended in Tris-Citrate-Egg Yolk and frozen as per the standard protocol...
February 2018: Animal Reproduction Science
B Balamurugan, S K Ghosh, S A Lone, J K Prasad, G K Das, R Katiyar, Abdul Rahman Mustapha, Ajay Kumar, M R Verma
The present study was designed to investigate the effect of partial deoxygenation of extender on sperm quality, lipid peroxidation (LPO) and reactive oxygen species (ROS) in buffalo (Bubalus bubalis) during cryopreservation of semen. Semen extender was prepared freshly and split into three sub-extenders [Extender I: control (non-deoxygenated), Extender II (partially deoxygenated by using LN2 flushing) and Extender III (partially deoxygenated mechanically by vacuum pump)]. Amounts of dissolved oxygen (DO) were determined in all the three extenders and also in post-thaw semen...
February 2018: Animal Reproduction Science
Yalçın Yaman, Cemal Ün
Resistance to bovine spongiform encephalopathy (BSE) that is significantly associated with insertion/deletion (indel) polymorphisms at two loci (putative promoter and intron 1) on the prion protein gene (PRNP) in cattle has been well documented. Studies suggest that the insertion alleles are related to BSE resistance. Until recently, BSE has never been reported in water buffaloes (unlike cattle). Previous studies have demonstrated that the PRNP gene in water buffalo consists mostly of insertion alleles at both loci; nevertheless, whether or not water buffaloes are genetically resistant to BSE and the role of indel polymorphisms in their resistance status is not clear...
March 2018: Tropical Animal Health and Production
A K Pandey, S P S Ghuman, G S Dhaliwal, M Honparkhe, J B Phogat, S Kumar
The present study was undertaken to investigate the effects of preovulatory follicle (POF) size on estradiol concentrations, luteal profile (CL diameter and progesterone concentration) and subsequent pregnancy rate in Murrah buffalo cows. The buffalo cows (n = 49) were synchronized for estrus by two doses of PGF2 α given 11 days apart. The buffalo cows were inseminated during standing estrus and again after 24 h. Ovaries were scanned at estrus and 24 h intervals until ovulation, thereafter on days 5, 12 and 16 post-ovulation to examine the POF and CL diameter...
February 2018: Theriogenology
O Aarif, A Aggarwal, A A Sheikh
Evaporative cooling during late gestation period improves post-partum reproductive performance in Murrah buffaloes. To prove this hypothesis, sixteen pregnant dry Murrah buffaloes at sixty days pre-partum were selected and divided into two groups of eight animals each. Group 1 of buffaloes (Cooled/CL) was managed under fan and mist cooling during dry period, whereas second group of buffaloes (non-cooled/NCL) remained without the provision of cooling. After parturition, all the animals were managed under evaporative cooling till the end of experimental period...
February 2018: Reproduction in Domestic Animals, Zuchthygiene
B C Divyashree, Sudhir C Roy
Binder of sperm-5 (BSP-5) is one of the fertility-associated proteins of cattle seminal plasma. Binding of sperm to the oviductal epithelium is mediated by BSP group of proteins. However, it is not clear, whether this protein is also involved in sperm motility. In the present study, attempts were made to characterize BSP-5 protein in both normozoospermic (NS) and asthenozoospermic (AS) Murrah buffalo (n = 18; Bubalus bubalis), Holstein Friesian (n = 8, Bos taurus) and Jersey cattle (n = 8; Bos taurus) bull seminal plasma and also study its expression pattern in these species...
October 16, 2017: Theriogenology
V K Sharma, S S Kundu, C Datt, S Prusty, M Kumar, U B Sontakke
This study was conducted to evaluate the utilisation of the residual feed intake (RFI) as a feed efficiency selection tool and its relationship with methane emissions. Eighteen Murrah buffalo (Bubalus bubalis) heifers were fed ad libitum with total mixed ration (TMR) for 120 days. Based on linear regression models involving dry matter intake (DMI), average daily gain (ADG) and mid-test metabolic body size (MBW0.75 ), heifers were assigned into low and high RFI groups. The RFI varied from -0.09 to +0.12 kg DM/day with average RFI of -0...
April 2018: Journal of Animal Physiology and Animal Nutrition
Vimlesh Kumar, Arumugam Kumaresan, Puneeth Kumar D S, Sreela Lathika, Samiksha Nayak, Kaustubh Kishor Saraf, Pradeep Nag B S, Shivani Chhillar, Tirtha Kumar Datta, Tushar Kumar Mohanty
An endocannabinoid system comprising of Anandamide (AEA) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. In the present study, we assessed the effect of sperm pre-treatment with AEA or co-incubation of sperm-oviduct explants with AEA in the presence or absence of CB1 receptor antagonist (SR141716A) on sperm-oviduct binding in the water buffalo. Cryopreserved spermatozoa from 3 Murrah buffalo bulls (3 ejaculates from each bull) were utilized for the study...
October 2017: Animal Reproduction Science
Maneesh Kumar Ahirwar, Mukund Amritrao Kataktalware, Kerekoppa Puttaiah Ramesha, Heartwin Amaladhas Pushpadass, Sakthivel Jeyakumar, Deginal Revanasiddu, Reen Jagish Kour, Sapna Nath, Anand Kumar Nagaleekar, Sayyad Nazar
The aim of the present study was to examine the effects of non-genetic factors on scrotal thermographic profile viz., proximal pole temperature (PPT °C), mid pole temperature (MPT °C), distal pole temperature (DPT °C) and ocular temperature (OcT) of Murrah (Bubalus bubalis) breeding bulls. A total of 109 buffalo bulls, maintained at three semen stations (SS), were monitored for scrotal surface and ocular temperatures using infrared thermography twice daily during rainy, winter and summer seasons using an FLIR i5 infrared camera and temperatures were measured...
December 2017: International Journal of Biometeorology
Harikrishna Pillai, Mehtab S Parmar, Ashish M Shende, Jobin Thomas, Harikumar S Kartha, Taru Sharma G, Subrata K Ghosh, Arumugam Kumaresan, Sanjeev K Bhure
Elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. Regucalcin (RGN), also known as Senescence marker protein-30 (SMP-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. In Drosophila, RGN is reportedly a putative cold-tolerance gene and a cytoprotective role for RGN against intracellular calcium elevation and oxidative stress was reported in P19 cell lines...
August 7, 2017: Molecular Reproduction and Development
M Gu, G Cosenza, I Nicolae, A Bota, Y Guo, L Di Stasio, A Pauciullo
Buffalo DGAT1 (diacylglycerol O-acyltransferase 1) was mainly investigated for the characterization of the gene itself and for the identification of the K232A polymorphism, similar to what has been accomplished in cattle, although no information has been reported so far at the mRNA level. The importance of DGAT1 for lipid metabolism led us to investigate the transcript profiles of lactating buffaloes characterized as high (9.13 ± 0.23) and low (7.94 ± 0.29) for milk fat percentage, and to explore the genetic diversity at the RNA and DNA level...
August 2, 2017: Journal of Dairy Science
S Yotov, A Atanasov
This study was designed to evaluate the dominant follicles development and the estradiol-17β concentrations in non-ovulating and ovulating post-partum buffaloes. Sixteen Bulgarian Murrah buffaloes were submitted to transrectal ultrasonographic examination from the 1st post-partum day until day 50, 3 days apart. The follicular diameter of the different categories of follicles and the ovulations was recorded. The animals were allocated into two groups: I (n = 6) non-ovulating and II (n = 10) ovulating buffaloes...
February 2018: Reproduction in Domestic Animals, Zuchthygiene
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