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https://www.readbyqxmd.com/read/28096623/estrus-induction-and-fertility-response-following-different-treatment-protocols-in-murrah-buffaloes-under-field-conditions
#1
L Kumar, J B Phogat, A K Pandey, S K Phulia, S Kumar, J Dalal
AIM: The aim of this study was to evaluate the efficacy of three different treatment protocols for estrus induction and conception rate in postpartum anestrus buffaloes during breeding season under field conditions. MATERIALS AND METHODS: The 47 postpartum anestrus buffaloes of the 2(nd) to 6(th) parity were divided into three groups. Group 1 (n=16): Buffaloes received cosynch treatment, that is, buserelin acetate 10 µg on day 0 and 9, cloprostenol 500 µg on day 7 followed by fixed-time artificial insemination (FTAI) at the time of second buserelin acetate and 24 h later...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28026086/identification-of-serum-protein-markers-for-early-diagnosis-of-pregnancy-in-buffalo
#2
Lukumoni Buragohain, Trilok Nanda, Arnab Ghosh, Mayukh Ghosh, Rajesh Kumar, Sunil Kumar, Sambhu Sharan Gupta, Arpita Bharali, Ashok K Mohanty, Inderjeet Singh, Ashok Kumar Balhara
Improper or delayed pregnancy diagnosis has significant impact over animal production, particularly in buffaloes which inherently suffer from several reproductive inefficiencies. Thus the present study has undertaken to identify serum protein markers pertaining to early pregnancy diagnosis in buffaloes. Serum samples were collected from 10 pregnant Murrah Buffalo heifers at weekly intervals from days 0-35 post-artificial insemination and from 12 inseminated non-pregnant cyclic buffalo heifers on days 0, 7, 14 and 21...
December 27, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28011259/exploration-of-luteinizing-hormone-in-murrah-buffalo-bubalus-bubalis-urine-extended-surge-window-opens-door-for-estrus-prediction
#3
Ramu Muthu Selvam, Suneel Kumar Onteru, Varij Nayan, Muthusamy Sivakumar, Dheer Singh, Govindaraju Archunan
Estrus detection in buffaloes has been a major concern for decades, and lack of reliable methods affects their effective reproductive management. Luteinizing hormone (LH) detection in urine is in practice for several mammals for timed insemination, whereas very few reports are available on buffalo urinary LH. The focus of this study is to detect the presence of LH in buffalo urine, quantitate variation in urinary LH during different estrous cycle phases and examine the duration of mid-cycle LH window. Nearly hundred buffaloes were examined, longitudinal urine samples (n=42) were collected from seventeen animals and classified into respective phases based on several estrus detection parameters...
December 21, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28004872/random-regression-models-to-estimate-genetic-parameters-for-weights-in-murrah-buffaloes
#4
Flavia Rita Ferreira, Francisco Ribeiro de Araujo Neto, Henrique Barbosa Borges, Rusbel Raul Aspilcueta-Borquis, Naudim Alejandro Hurtado-Lugo, Henrique Nunes de Oliveira, Lucia Galvão de Albuquerque, Humberto Tonhati
This article reports genetic analysis of the weight at different ages of Murrah water buffaloes, using random regression models (RRM). Models ranging from third to sixth order polynomial were used to describe direct genetic and animal permanent environmental effects. Contemporary group was included as a fixed effect, and a cubic polynomial was used to model the mean curve of the population. The residual was modeled considering a log-linear function. Two models were selected for study of genetic parameters. The first model included third and sixth order polynomials for direct genetic and animal permanent environmental effects (M36)...
December 22, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27847429/evaluation-of-salivary-electrolytes-during-estrous-cycle-in-murrah-buffaloes-with-reference-to-estrus-detection
#5
Indu Devi, Pawan Singh, Surerder Singh Lathwal, A Kumaresan, Kuldeep Dudi
AIM: Timely estrus detection is one of the critical factors for increasing reproductive efficiency in buffaloes. In recent decades, saliva has become a more popular as a noninvasive source for determining physiological status of animals by various biochemical electrolytes. This study was designed to assess and correlate changes in different salivary minerals concentration (calcium, inorganic phosphorus, magnesium, sodium, potassium, and chloride) during different stages of the estrous cycle in Murrah buffaloes...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27822596/investigation-of-the-prion-protein-gene-prnp-polymorphisms-in-anatolian-murrah-and-crossbred-water-buffaloes-bubalus-bubalis
#6
Yalçın Yaman, Orhan Karadağ, Cemal Ün
Bovine spongiform encephalopathy (BSE) of the cattle is the outstanding disease among other transmissible spongiform encephalopathy (TSEs). It can be transmitted from the cattle to a human and causes a new variant of the Creutzfeldt-Jakob disease (CJD). It is known that prion protein coding gene (PRNP) plays a major role in the TSE susceptibility or resistance in some species. Recent researches demonstrated that the insertion (in) and deletion (del) polymorphisms within promoter and intron 1 region of the PRNP related to BSE susceptibility in cattle...
November 8, 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27706739/polymorphisms-in-the-dgat1-gene-in-buffaloes-bubalus-bubalis-in-the-amazon
#7
C S Silva, E Silva Filho, A S Matos, A S Schierholt, M R Costa, L C Marques, J S Costa, R L Sales, M R Figueiró, J R F Marques
Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production...
September 16, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27687066/four-novel-polymorphisms-of-buffalo-insig2-gene-are-associated-with-milk-production-traits-in-chinese-buffaloes
#8
Tingxian Deng, Chunying Pang, Xiaoya Ma, Xingrong Lu, Anqin Duan, Peng Zhu, Xianwei Liang
Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals...
September 27, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27666680/nutrient-utilisation-growth-performance-and-blood-metabolites-in-murrah-buffalo-calves-bubalus-bubalis-divergently-selected-for-residual-feed-intake
#9
Vijay K Sharma, Shivlal S Kundu, Sonali Prusty, Chander Datt, Muneendra Kumar
The aim of this study was to evaluate differences in efficiency of feed utilisation between buffalo calves with low and high residual feed intake (RFI) by comparing feed intake, nutrient digestibility, growth traits and blood metabolites. Eighteen male Murrah buffalo calves (aged 4-6 months; 70 ± 1.0 kg body weight) were fed ad libitum with a total mixed ration for 120 d. Based on linear regression models involving dry matter intake (DMI), average daily gain (ADG) and mid-test metabolic body size, calves were assigned into low and high RFI groups...
December 2016: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/27631328/estimation-of-mineral-and-trace-element-profile-in-bubaline-milk-affected-with-subclinical-mastitis
#10
Mahavir Singh, Poonam Yadav, Anshu Sharma, V K Garg, Dinesh Mittal
The milk samples from buffaloes of Murrah breed at mid lactation stage, reared at an organised dairy farm, were screened for subclinical mastitis based on bacteriological examination and somatic cell count following International Dairy Federation criteria. Milk samples from subclinical mastitis infected and healthy buffaloes were analysed to evaluate physicochemical alterations in terms of protein, fat, pH, electrical conductivity, chloride, minerals (sodium, potassium and calcium) and trace elements (iron, zinc, copper and selenium)...
September 9, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27620867/variability-in-residual-feed-intake-and-nutrient-utilization-in-murrah-buffalo-heifers
#11
Tegene Negesse, Chander Datt, S S Kundu
A feeding trial with 18 Murrah buffalo heifers (238 ± 63 kg; 1.7 ± 0.4 years old) was conducted for 57 days, and feed intake and body weight were recorded followed by 7 days of urine and feces collection. Heifers were grouped into low (RFI-I) and high (RFI-II) feed efficiency groups by regressing DMI (g head(-1) day(-1)) on BWG (g head(-1) day(-1)) and mid-test MBW (kg). RFI-II heifers had slightly higher DMI (g head(-1) day(-1) and expressed per unit of BW, %BW, MBW, mid-test BW, and mid-test MBW) and all associated nutrient intakes, BWG, feed efficiency traits (FCR, CPCR), digestibility of nutrients, and retentions of crude protein and fat over RFI-I heifers...
December 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27605773/effect-of-induced-fasciola-gigantica-infection-during-pre-patency-on-the-performance-of-buffalo-calves-fed-on-different-percentage-of-protein
#12
P Singh, A K Verma, S C Gupta, U R Mehra
Thirty growing Murrah buffalo calves (8-12 months of age, 109.85 ± 2.43) were reared in parasite free conditions and randomly divided into three equal groups as per CRD. They were fed on iso-caloric (2.01 ME Mcal/Kg diet) diets containing standard protein (SP) diet at 100 %, 90 % of SP (medium protein, MP) and 80 % of SP (low protein, LP) of the protein requirements (Kearl 1982). After 21 days of feeding, each group was further subdivided into two sub-groups (A & B). Animals in sub-groups 'A' served as non-infected control, while in sub group 'B' were orally infected with Fasciola gigantica metacercarie (mc; 1,000 each)...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27600966/separation-and-quantification-of-milk-casein-from-different-buffalo-breeds
#13
Shanshan Li, Ling Li, Qingkun Zeng, Jianxin Liu, Daxi Ren
Understanding the milk protein expression profile in different buffalo breeds plays an important role in improving hybrid selection and determining the effects on milk protein synthesis. The aim of this research is to compare the differences in milk protein content, composition and distribution between River buffalo and their crossbreeds for hybrid screening. Four groups of milk samples that included Nili-Ravi (N), Murrah (M), a Nili-Ravi-Murrah crossbreed (M-N), and a crossbreed of river buffalo with local swamp buffalo (C) were collected...
August 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27555524/identification-of-suitable-combinations-of-in%C3%A2-vitro-sperm-function-test-for-the-prediction-of-fertility-in-buffalo-bull
#14
Raushan K Singh, A Kumaresan, Shivani Chhillar, Shailendra K Rajak, Utkarsh K Tripathi, Samiksha Nayak, T K Datta, T K Mohanty, R Malhotra
The present study assessed sperm functional characteristics in the frozen-thawed semen of buffalo bulls and estimated their relationship with field fertility. Frozen semen samples from three different freezing operations each from nine Murrah buffalo bulls were used for the assessment of different sperm functions related to fertilizing potential. Bulls were classified into high (n = 2), medium (n = 5), and low (n = 2) fertile based on adjusted field fertility. The sperm functions estimated included membrane integrity using carboxyfluorescein diacetate-propidium iodide, acrosome reaction status using fluorescein isothiocyanate peanut agglutinine, status of apoptosis using Annexin-V, protamine deficiency using Chromomycin A3, membrane stability using Merocyanine 540 and lipid peroxidation status using 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene...
December 2016: Theriogenology
https://www.readbyqxmd.com/read/27491308/ameloblastoma-phenotypes-reflected-in-distinct-transcriptome-profiles
#15
Shijia Hu, Joel Parker, Kimon Divaris, Ricardo Padilla, Valerie Murrah, John Timothy Wright
Ameloblastoma is a locally invasive benign neoplasm derived from odontogenic epithelium and presents with diverse phenotypes yet to be characterized molecularly. High recurrence rates of 50-80% with conservative treatment in some sub-types warrants radical surgical resections resulting in high morbidity. The objective of the study was to characterize the transcriptome of ameloblastoma and identify relevant genes and molecular pathways using normal odontogenic tissue (human "dentome") for comparison. Laser capture microdissection was used to obtain neoplastic epithelial tissue from 17 tumors which were examined using the Agilent 44 k whole genome microarray...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27469895/genetic-association-between-snps-in-the-dgat1-gene-and-milk-production-traits-in-murrah-buffaloes
#16
Ana Cláudia de Freitas, Gregório Miguel Ferreira de Camargo, Nedenia Bonvino Stafuzza, Rusbel Raul Aspilcueta-Borquis, Guilherme Costa Venturini, Marina Mortati Dias, Diercles Francisco Cardoso, Humberto Tonhati
This study identified polymorphisms in the DGAT1 gene in Murrah buffaloes and investigated the associations to milk production and quality traits (milk, fat and protein yields and percentages, somatic cell count). Genomic DNA was extracted from hair follicles collected from the tail of 196 females. Three SNPs were identified in DGAT1 gene by sequencing. Statistical analyses were performed to verify the linkage and the association between polymorphisms and traits. The estimated value of r (2) between two SNPs in exon 17 (g...
October 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27448191/meat-quality-of-buffaloes-finished-in-traditional-or-silvopastoral-system-in-the-brazilian-eastern-amazon
#17
Maria Regina Sarkis Peixoto Joele, Lúcia de Fátima Henriques Lourenço, José de Brito Lourenço Júnior, Geisielly Sousa Araujo, Juliana Cristina de Castro Budel, Alexandre Rossetto Garcia
BACKGROUND: This study aimed to assess the physical, chemical, and sensory characteristics of meat from buffaloes finished in a Traditional System (TS) or Silvopastoral System (TSPS) with dietary supplementation. Crossbred Murrah x Mediterranean buffaloes were raised from weaning to slaughter in the TS (n = 15) or were raised in the traditional system and finished in the TSPS (n = 15). After finishing, animals were slaughtered and their carcasses refrigerated for 24 h. The right side of each half-carcass was cut between the 12(th) and 13(th) ribs and the Longissimus thoracis muscle was removed...
July 22, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27413298/epidemiological-and-diagnostic-studies-of-microfilariasis-in-buffaloes
#18
V Samatha, Ch Jyothisree, K Ramesh Babu
A study was conducted to determine the prevalence of microfilariasis and analysis of diagnostic methods in buffaloes at Veterinary Poly Clinic, Gudiwada with relation to season, age and breed for a period of 1 year (March 2011-February 2012).Out of 1,222 blood smears examination, only 123 samples were found positive for microfilariasis with a prevalence rate of 10.06 %. Highest prevalence was observed in monsoon (12.54 %) followed by summer (9.18 %) and least in winter (7.05 %). Buffaloes above 9 years age were at high risk with a prevalence rate of 12...
June 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27397983/effect-of-egg-yolk-powder-on-freezability-of-murrah-buffalo-bubalus-bubalis-semen
#19
N Kumar, S A Lone, J K Prasad, M H Jan, S K Ghosh
AIM: The aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of Murrah buffalo semen. MATERIALS AND METHODS: Semen samples (12) from 3 Murrah buffaloes (4 from each bull) with mass motility (≥3+) and total motility (70% and above) were utilized in this study. Immediately after collection, each sample was divided into four groups. Groups I was diluted up to 60×10(6) sperm/ml with tris extender containing 10% fresh egg yolk and Groups II, III, and IV were diluted up to 60×10(6) sperm/ml with tris extender containing 2%, 4%, and 6% egg yolk powder, respectively...
June 2016: Veterinary World
https://www.readbyqxmd.com/read/27357943/evaluation-of-fertility-in-relation-to-milk-production-and-productivity-of-murrah-buffaloes
#20
V Jamuna, A K Chakravarty
Intense selection of buffaloes for milk production at organized herds of the country without giving due attention to fertility traits has lead to deterioration in their performances. The study was initiated to assess the relationship between milk production and productivity with fertility in Murrah buffaloes. In the present study, fertility was defined in terms of pregnancy rate of Murrah buffaloes. Pregnancy rate measures the percentage of non-pregnant animals that become pregnant during each oestrous cycle...
August 2016: Animal Reproduction Science
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