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https://www.readbyqxmd.com/read/29775750/the-effects-of-capn1-gene-inactivation-on-the-differential-expression-of-genes-in-skeletal-muscle
#1
William T Olive, Brittney N Keel, Amanda K Lindholm-Perry, Justyna Horodyska, Andrew P Foote
Protein turnover is required for muscle growth and regeneration and several proteolytic enzymes, including the calpains, degrade myofibrillar proteins during this process. In a previous experiment, phenotypic differences were observed between μ-calpain knockout (KO) and wild type (WT) mice, including nutrient accretion and fiber type differences. These changes were particularly evident as the animals aged. Thus, we utilized 18 mice (9 KO and 9 WT) to compare transcript abundance to identify differentially expressed genes (DEGs) at 52 wk of age...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29772099/where-is-the-residual-terrestrial-carbon-sink
#2
R A Houghton, A Baccini, W S Walker
The apparent accumulation of carbon on land necessary to balance the global carbon budget (previously referred to as the missing carbon sink and more recently labeled the residual terrestrial sink) has perplexed scientists since the first carbon budgets were constructed (Keeling 1973). The magnitude of the sink over the decade 2006-2015 averaged 3.1 ± 0.9 PgC yr-1 , larger than either the net emissions of carbon from land-use change or the net uptake by the world's oceans (Le Quéré et al., 2016). This article is protected by copyright...
May 17, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29765018/tracking-hiv-1-recombination-to-resolve-its-contribution-to-hiv-1-evolution-in-natural-infection
#3
Hongshuo Song, Elena E Giorgi, Vitaly V Ganusov, Fangping Cai, Gayathri Athreya, Hyejin Yoon, Oana Carja, Bhavna Hora, Peter Hraber, Ethan Romero-Severson, Chunlai Jiang, Xiaojun Li, Shuyi Wang, Hui Li, Jesus F Salazar-Gonzalez, Maria G Salazar, Nilu Goonetilleke, Brandon F Keele, David C Montefiori, Myron S Cohen, George M Shaw, Beatrice H Hahn, Andrew J McMichael, Barton F Haynes, Bette Korber, Tanmoy Bhattacharya, Feng Gao
Recombination in HIV-1 is well documented, but its importance in the low-diversity setting of within-host diversification is less understood. Here we develop a novel computational tool (RAPR (Recombination Analysis PRogram)) to enable a detailed view of in vivo viral recombination during early infection, and we apply it to near-full-length HIV-1 genome sequences from longitudinal samples. Recombinant genomes rapidly replace transmitted/founder (T/F) lineages, with a median half-time of 27 days, increasing the genetic complexity of the viral population...
May 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29763484/perianal-condyloma-acuminatum-like-plaque
#4
Ying Luo, Brett Keeling, Jessica A Forcucci, Dirk M Elston
No abstract text is available yet for this article.
April 2018: Cutis; Cutaneous Medicine for the Practitioner
https://www.readbyqxmd.com/read/29758060/genetic-variation-in-humoral-response-to-an-escherichia-coli-o157-h7-vaccine-in-beef-cattle
#5
Kara B Marley, Larry A Kuehn, John W Keele, Benjamin W Wileman, Michael G Gonda
Individuals often respond differently to the same vaccine; some of this variation may be caused by genetic differences among animals. Our objective was to estimate heritability and identify genomic regions associated with humoral response to an Escherichia coli O157:H7 vaccine in beef cattle. Crossbred beef cattle (n = 651) were vaccinated with a commercially available E. coli O157:H7 vaccine. Serum was collected at time of initial vaccination (d 0), booster (d 21), and d 56 after initial vaccination. Total antibodies specific to siderophore receptor and porin proteins in the vaccine were quantified by enzyme-linked immunosorbent assay...
2018: PloS One
https://www.readbyqxmd.com/read/29749920/evolution-of-the-graded-repair-of-csf-leaks-and-skull-base-defects-in-endonasal-endoscopic-tumor-surgery-trends-in-repair-failure-and-meningitis-rates-in-509-patients
#6
Andrew Conger, Fan Zhao, Xiaowen Wang, Amalia Eisenberg, Chester Griffiths, Felice Esposito, Ricardo L Carrau, Garni Barkhoudarian, Daniel F Kelly
OBJECTIVE The authors previously described a graded approach to skull base repair following endonasal microscopic or endoscope-assisted tumor surgery. In this paper they review their experience with skull base reconstruction in the endoscopic era. METHODS A retrospective review of a single-institution endonasal endoscopic patient database (April 2010-April 2017) was undertaken. Intraoperative CSF leaks were graded based on size (grade 0 [no leak], 1, 2, or 3), and repair technique was documented across grades...
May 11, 2018: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29742164/radiographic-examination-of-keel-bone-damage-in-living-laying-hens-of-different-strains-kept-in-two-housing-systems
#7
Beryl Katharina Eusemann, Ulrich Baulain, Lars Schrader, Christa Thöne-Reineke, Antonia Patt, Stefanie Petow
A high prevalence of deviations and fractures of the keel bone is a widespread welfare problem in laying hens. The aim of this study was to experimentally investigate this multifactorial problem throughout the laying period and to compare the prevalence and severity in different layer lines and different housing systems. High performing white (WLA) and brown (BLA) pure bred layer lines and low performing white (R11, G11) and brown layer lines (L68) were kept in both single cages and a floor housing system. A total of 97 hens (19 or 20 from each line, respectively) were repeatedly radiographed in the 35th, 51st and 72nd week of age...
2018: PloS One
https://www.readbyqxmd.com/read/29727060/eukref-ciliophora-a-manually-curated-phylogeny-based-database-of-small-subunit-rrna-gene-sequences-of-ciliates
#8
Vittorio Boscaro, Luciana F Santoferrara, Qianqian Zhang, Eleni Gentekaki, Mitchell J Syberg-Olsen, Javier Del Campo, Patrick J Keeling
High-throughput sequencing (HTS) surveys, among the most common approaches currently used in environmental microbiology, require reliable reference databases to be correctly interpreted. The EukRef Initiative (eukref.org) is a community effort to manually screen available small subunit (SSU) rRNA gene sequences and produce a public, high-quality, and informative framework of phylogeny-based taxonomic annotations. In the context of EukRef, we present a database for the monophyletic phylum Ciliophora, one of the most complex, diverse, and ubiquitous protist groups...
May 4, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29724382/chronic-airway-obstruction-in-a-population-based-adult-asthma-cohort-prevalence-incidence-and-prognostic-factors
#9
Helena Backman, Sven-Arne Jansson, Caroline Stridsman, Hana Muellerova, Keele Wurst, Linnea Hedman, Anne Lindberg, Eva Rönmark
BACKGROUND: Asthma and COPD may overlap (ACO) but information about incidence and risk factors are lacking. This study aimed to estimate prevalence, incidence and risk factors of chronic airway obstruction (CAO) in a population-based adult asthma cohort. METHODS: During 1986-2001 a large population-based asthma cohort was identified (n = 2055, 19-72y). Subsamples have participated in clinical follow-ups during the subsequent years. The entire cohort was invited to a clinical follow-up including interview, spirometry, and blood sampling in 2012-2014 when n = 983 subjects performed adequate spirometry...
May 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29722144/is-the-number-and-size-of-scales-in-liolaemus-lizards-driven-by-climate
#10
María José Tulli, Félix B Cruz
Ectothermic vertebrates are sensitive to thermal fluctuations in the environments where they occur. To buffer these fluctuations, ectotherms use different strategies, including the integument, which is a barrier that minimizes temperature exchange between the inner body and the surrounding air. In lizards, this barrier is constituted by keratinized scales of variable size, shape and texture, and its main function is protection, water loss avoidance and thermoregulation. The size of scales in lizards has been proposed to vary in relation to climatic gradients; however, it has also been observed that in some groups of Iguanian lizards could be related to phylogeny...
May 3, 2018: Integrative Zoology
https://www.readbyqxmd.com/read/29720516/seminal-siv-in-chronically-infected-cynomolgus-macaques-is-dominated-by-virus-originating-from-multiple-genital-organs
#11
Laurent Houzet, Marcos Pérez-Losada, Giulia Matusali, Claire Deleage, Nathalie Dereuddre-Bosquet, Anne-Pascale Satie, Florence Aubry, Emmanuelle Becker, Bernard Jégou, Roger Le Grand, Brandon F Keele, Keith A Crandall, Nathalie Dejucq-Rainsford
The sexual transmission of viruses is responsible for the spread of multiple infectious diseases. Although the HIV/AIDS pandemic remains fueled by sexual contacts with infected semen, the origin of virus in semen is still unknown. In a substantial number of HIV- infected men, viral strains present in semen differ from the ones in blood, suggesting that HIV is locally produced within the genital tract. Such local production may be responsible for the persistence of HIV in semen despite effective antiretroviral therapy...
May 2, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29718937/routine-activities-and-emotion-in-the-life-of-dairy-cows-integrating-body-language-into-an-affective-state-framework
#12
Daiana de Oliveira, Linda J Keeling
We assessed dairy cows' body postures while they were performing different stationary activities in a loose housing system and then used the variation within and between individuals to identify potential connections between specific postures and the valence and arousal dimensions of emotion. We observed 72 individuals within a single milking herd focusing on their ear, neck and tail positions while they were: feeding from individual roughage bins, being brushed by a mechanical rotating brush and queuing to enter a single automatic milking system...
2018: PloS One
https://www.readbyqxmd.com/read/29716244/a-frame-selective-dynamic-programming-approach-for-noise-robust-pitch-estimation
#13
Chiranjeevi Yarra, Om D Deshmukh, Prasanta Kumar Ghosh
The principles of the existing pitch estimation techniques are often different and complementary in nature. In this work, a frame selective dynamic programming (FSDP) method is proposed which exploits the complementary characteristics of two existing methods, namely, sub-harmonic to harmonic ratio (SHR) and sawtooth-wave inspired pitch estimator (SWIPE). Using variants of SHR and SWIPE, the proposed FSDP method classifies all the voiced frames into two classes-the first class consists of the frames where a confidence score maximization criterion is used for pitch estimation, while for the second class, a dynamic programming (DP) based approach is proposed...
April 2018: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/29707861/perioperative-replacement-therapy-in-haemophilia-b-an-appeal-to-b-more-precise
#14
H C A M Hazendonk, T Preijers, R Liesner, P Chowdary, D Hart, D Keeling, M H E Driessens, B A P Laros-van Gorkom, F J M van der Meer, K Meijer, K Fijnvandraat, F W G Leebeek, R A A Mathôt, P W Collins, M H Cnossen
INTRODUCTION: Haemophilia B is caused by a deficiency of coagulation factor IX (FIX) and characterized by bleeding in muscles and joints. In the perioperative setting, patients are treated with FIX replacement therapy to secure haemostasis. Targeting of specified FIX levels is challenging and requires frequent monitoring and adjustment of therapy. AIM: To evaluate perioperative management in haemophilia B, including monitoring of FIX infusions and observed FIX levels, whereby predictors of low and high FIX levels were assessed...
April 29, 2018: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/29701793/conservation-tillage-and-organic-farming-induce-minor-variations-in-pseudomonas-abundance-their-antimicrobial-function-and-soil-disease-resistance
#15
Francesca Dennert, Nicola Imperiali, Cornelia Staub, Jana Schneider, Titouan Laessle, Tao Zhang, Raphaël Wittwer, Marcel G A van der Heijden, Theo H M Smits, Klaus Schlaeppi, Christoph Keel, Monika Maurhofer
Conservation tillage and organic farming are strategies used worldwide to preserve the stability and fertility of soils. While positive effects on soil structure have been extensively reported, the effects on specific root- and soil-associated microorganisms are less known. The aim of this study was to investigate how conservation tillage and organic farming influence the frequency and activity of plant-beneficial pseudomonads. Amplicon sequencing using the 16S rRNA gene revealed that Pseudomonas is among the most abundant bacterial taxa in the root microbiome of field-grown wheat, independent of agronomical practices...
April 25, 2018: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29699984/prevalence-of-glaucoma-in-the-australian-national-eye-health-survey
#16
Stuart Keel, Jing Xie, Joshua Foreman, Pei Ying Lee, Mostafa Alwan, Eamonn T Fahy, Peter van Wijngaarden, Jennifer C Fan Gaskin, Ghee Soon Ang, Jonathan G Crowston, Hugh R Taylor, Mohamed Dirani
AIM: To estimate the prevalence of glaucoma in Australia. METHODS: This was a population-based study of 3098 non-Indigenous Australians (50-98 years) and 1738 Indigenous Australians (40-92 years) stratified by remoteness. Each participant underwent a standard examination that included visual field assessment, tonometry and non-mydriatic fundus photography. Two fellowship-trained glaucoma specialists independently assessed relevant case notes (past ocular history, best-corrected visual acuity, frequency doubling technology visual fields, Van Herick grade, intraocular pressure and optic disc-centred photographs) and assigned a diagnosis ranked on a scale of certainty: none, possible, probable or definite glaucoma...
April 26, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29696313/using-proprioception-to-control-ongoing-actions-dominance-of-vision-or-altered-proprioceptive-weighing
#17
Rachel Goodman, Luc Tremblay
Visual feedback is crucial for movement accuracy (e.g., Keele and Posner, J Exp Psychol 77:155-158, 1968). As well, proprioception has been argued to be important for the control of voluntary movements (e.g., Bagesteiro et al., Exp Brain Res 171:358-370, 2006; Rossetti et al., J Neurophysiol 74:457-463, 1995). While tendon vibration (TVib) has been used to perturb proprioceptive information during limb matching tasks (Goodwin et al., Science 175:1382-1384, 1972), the current study employed between-trial dual-muscle TVib, coupled with vision occlusion, to assess the use of vision and proprioception for the online control of voluntary movements...
April 25, 2018: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/29690459/taxonomic-status-of-tropiocolotes-cf-steudneri-with-a-description-of-a-new-species-of-tropiocolotes-reptilia-squamata-gekkonidae-in-southern-iran
#18
Mahdi Rajabizadeh, Hiva Faizi, Steven C Anderson, Mohammad Zarrintab, Roman Nazarov
We review the status of an Iranian gecko population previously referred to Tropiocolotes cf. steudneri and describe it as a new species, Tropiocolotes hormozganensis sp. nov. We discuss the taxonomic history of this population and compare its characters with those of the other species in the genus. The new species is distinguished from other species of Tropiocolotes by possessing weakly keeled dorsal scales and smooth ventral scales, having imbricate scales on dorsal and ventral tail, possessing clearly tricarinatesubdigital scales, 48-55 dorsal scales, two pairs of postmental shields, of which the second pair is about half of the size of the first, 100-107 scales longitudinally along underside of body, 15-19 scales across head and 16-19 subdigital lamellae...
March 1, 2018: Zootaxa
https://www.readbyqxmd.com/read/29690159/generic-assignment-of-leuciscus-kurui-bogutskaya-from-the-upper-tigris-drainage-and-a-replacement-name-for-alburnus-kurui-mangit-yerli-teleostei-leuciscidae
#19
JÖrg Freyhof, CÜneyt Kaya, Esra BayÇelebİ, Matthias Geiger, Davut Turan
The generic position of Leuciscus kurui Bogutskaya, 1995 is reviewed through a comparison of morphological and molecular characters (COI). The molecular data place L. kurui in Alburnus, close to Alburnus timarensis from the Lake Van basin. Alburnus kurui (Bogutskaya) is distinguished from this species by lacking a ventral keel and possessing both a very low number of gill rakers and midlateral scales. Alburnus selcuklui, from the upper Tigris drainage, cannot be distinguished from the widespread A. sellal and is therefore treated as a synonym of this species...
April 16, 2018: Zootaxa
https://www.readbyqxmd.com/read/29690116/a-new-species-of-the-genus-bryodemella-s-str-yin-1982-from-china-orthoptera-acridoidea-oedipodidae
#20
Yu-Long Zhang, Yong-Chao Zhi, Dao-Chuan Zhang
A new species (i.e. Bryodemella (s. str.) rufifemura sp. nov. from China is described in this paper. It is similar to Bryodemella (s. str.) diamesum (Bei-Bienko, 1930), but differs from the latter by red inner side of hind femur, median keel of pronotum indistinct in metazoan, and vertical diameter of eye shorter than subocular groove in female.  The type specimens are deposited in the Museum of Hebei University (MHU), China.
April 23, 2018: Zootaxa
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