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Metarhizium anisopliae knockout

Bruno Giuliano Garisto Donzelli, Stuart B Krasnoff, Alice C L Churchill, John D Vandenberg, Donna M Gibson
The fungal entomopathogen Metarhizium robertsii (formerly known as M. anisopliae var. anisopliae) is a prolific producer of secondary metabolites of which very little is known at the genetic level. To establish the genetic bases for the biosynthesis of the mutagenic compound NG- 391, we identified a 19,818 kb genomic region harboring the predicted hybrid polyketide synthase-nonribosomal peptide synthetase NGS1, plus five additional ORFs. NGS1 knockouts generated by Agrobacterium-mediated transformation failed to produce detectable levels of NG-391, indicating the involvement of this locus in its biosynthesis...
April 2010: Current Genetics
Weiguo Fang, Monica Pava-ripoll, Sibao Wang, Raymond St Leger
Metarhizium anisopliae is a model system for studying insect fungal pathogenesis. The role of cAMP signal transduction in virulence was studied by disrupting the class I PKA catalytic subunit gene (MaPKA1). The PKA mutant (DeltaMaPKA1) showed reduced growth and greatly reduced virulence. PKA was dispensable for differentiation of infection structures (appressoria), but differentiation was delayed and the appressoria were defective because of reduced turgor pressure. DeltaMaPKA1 germinated at similar rates as the wild type in glucose and glycerol, but germination was delayed on alanine...
March 2009: Fungal Genetics and Biology: FG & B
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