Michael A Paley, Brandi J Baker, S Richard Dunham, Nicole Linskey, Claudia Cantoni, Kenneth Lee, Lynn M Hassman, Jennifer Laurent, Elisha D O Roberson, David B Clifford, Wayne M Yokoyama
The tissue-specific drivers of neurosarcoidosis remain poorly defined. To identify cerebrospinal fluid (CSF) specific, antigen-driven T and B cell responses, we performed single-cell RNA sequencing of CSF and blood cells from neurosarcoid participants coupled to T and B cell receptor sequencing. In contrast to pulmonary sarcoidosis, which is driven by CD4 T cells, we found CD8 T cell clonal expansion enriched in the neurosarcoid CSF. These CSF-enriched CD8 T cells were composed of two subsets with differential expression of EBI2, CXCR3, and CXCR4...
June 15, 2022: Journal of Neuroimmunology