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https://www.readbyqxmd.com/read/28743271/development-of-inducer-free-expression-plasmids-based-on-iptg-inducible-promoters-for-bacillus-subtilis
#1
Dinh Thi Minh Tran, Trang Thi Phuong Phan, Thanh Kieu Huynh, Ngan Thi Kim Dang, Phuong Thi Kim Huynh, Tri Minh Nguyen, Tuom Thi Tinh Truong, Thuoc Linh Tran, Wolfgang Schumann, Hoang Duc Nguyen
BACKGROUND: Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and in B. subtilis. The ligation products are first transformed into E. coli cells, analyzed for correct insertions, and the correct recombinant plasmids are then transformed into B. subtilis. A major problem using E. coli cells can be the strong basal level of expression of the recombinant protein which may interfere with the stability of the cells...
July 25, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28730156/white-blood-cell-counts-alcoholism-and-cirrhosis-in-pneumococcal-pneumonia
#2
Julianna G Gardner, Divya R Bhamidipati, Adriana M Rueda, Duc T M Nguyen, Edward A Graviss, Daniel M Musher
BACKGROUND: An elevated white blood cell (WBC) count is a characteristic finding in pneumococcal pneumonia. Very low WBC counts, occurring in some cases, are often associated with overwhelming pneumonia and have been attributed to alcohol-induced suppression of bone marrow. However, a systematic study of neutropenia, leukocytosis, alcohol ingestion, and cirrhosis in pneumococcal pneumonia has not been previously reported. METHODS: Using a database of patients with pneumococcal pneumonia at our medical center, we extracted data on WBC counts at admission, differential counts, alcohol ingestion, and cirrhosis, and we related these to 7-day and 30-day mortality...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28726708/potential-of-integrated-vertical-and-horizontal-flow-constructed-wetland-with-native-plants-for-sewage-treatment-under-different-hydraulic-loading-rates
#3
Xuan Cuong Nguyen, Dinh Duc Nguyen, Nguyen Thi Loan, Soon Woong Chang
In this study, a pilot-scale integrated constructed wetland with vertical flow (VF) and horizontal flow (HF) in series was designed and investigated to evaluate sewage wastewater treatment capacity. The VF unit was planted with Canna indica and was 1.2 m long, 1.2 m wide, and 1.2 m high; whereas the HF unit contained Colocasia esculenta and was 3.0 m long, 1.0 m wide, and 1.0 m high. The system was operated under different hydraulic loading rates (HLRs) of 0.1, 0.2, and 0.15 m/d. The effluent concentrations differed as HLR changed, and the means were total suspended solids (TSS): 87 mg/L; biological oxygen demand (BOD5): 31 mg/L; chemical oxygen demand (CODCr): 59 mg/L; ammonium nitrogen (NH4-N): 5...
July 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28723909/reliability-convergent-validity-and-factor-structure-of-the-dass-21-in-a-sample-of-vietnamese-adolescents
#4
Minh Thi Hong Le, Thach Duc Tran, Sara Holton, Huong Thanh Nguyen, Rory Wolfe, Jane Fisher
OBJECTIVES: To assess the internal consistency, latent structure and convergent validity of the Depression, Anxiety and Stress Scale-21 (DASS-21) among adolescents in Vietnam. METHOD: An anonymous, self-completed questionnaire was conducted among 1,745 high school students in Hanoi, Vietnam between October, 2013 and January, 2014. Confirmatory factor analyses were performed to assess the latent structure of the DASS-21. Factorial invariance between girls and boys was examined...
2017: PloS One
https://www.readbyqxmd.com/read/28719777/sex-ratio-at-birth-in-vietnam-results-from-data-in-chililab-hdss-2004-to-2013
#5
Vui Thi Le, Duc Minh Duong, Anh Duy Nguyen, Chuong Canh Nguyen, Ha Thi Thu Bui, Cuong Viet Pham, Thi Minh Le, Bich Huu Tran
This study aimed to explore the association of demographic and socioeconomic characteristics and imbalanced sex ratio at birth (SRB) in Chi Linh district, Hai Duong. The data were collected from a longitudinal study using a community-based periodic, referred as Chi Linh Health and Demographic Surveillance System (CHILILAB HDSS) during 2004 to 2013. A total of 7568 children were analyzed. Results showed that SRB in Chi Linh dramatically increased to the imbalanced sex ratio (114.6 boys to 100 girls) by 2013...
July 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28719774/a-secular-trend-in-birth-weight-and-delivery-practices-in-periurban-vietnam-during-2005-2012
#6
Duc Minh Duong, Anh Duy Nguyen, Chuong Canh Nguyen, Vui Thi Le, Son Ngoc Hoang, Ha Thi Thu Bui
The remarkable increase in Vietnamese economic conditions can increase the birth weight in neonates and better delivery practices among women. The Chi Linh Health and Demographic Surveillance System started in 2004. An open cohort of data consisting of about 57 561 people from 17 993 households has been followed primarily with respect to demography, economy, and education. The aim of this research is to study secular trends in delivery practice and birth weight in the past decade (2005-2012) in Chi Linh...
July 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28719773/methods-for-the-2016-socioeconomic-and-health-survey-in-chi-linh-health-and-demographic-surveillance-system-chililab-hdss
#7
Hoang Van Minh, Tran Thu Ngan, Nguyen Bao Ngoc, Vu Duy Kien, Vu Quynh Mai, Tran Bich Phuong, Tran Tuan Anh, Kim Bao Giang, Phan Van Tuong, Ha Anh Duc, Do Van Dung
The Chi Linh Health and Demographic Surveillance System (CHILILAB HDSS) was established in 2004 in Chi Linh District, Hai Duong Province (Northern Vietnam). Up to 2013, 22 rounds of data collection at CHILILAB HDSS had been completed. This article reports the methods and key sociodemographic characteristics of households and individuals captured by the survey conducted among the subsamples of CHILILAB HDSS in 2016. We observed and compared them to the previous HDSS survey rounds and found no significant differences for household size and gender compositions in CHILILAB HDSS...
July 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28719770/household-exposure-to-livestock-and-health-in-the-chililab-hdss-cohort-vietnam
#8
Sinh Dang-Xuan, Lauren E MacDonald, Janna M Schurer, Hung Nguyen-Viet, Phuc Pham-Duc
In Vietnam, pigs and poultry are predominantly produced by small-scale farmers, creating challenges for zoonotic disease management. The objective of this study was to characterize practices related to livestock and manure management and to measure association with 3 self-reported health symptoms (coughing, fever, and diarrhea/nausea/vomiting) in a region currently undergoing health transitions. We analyzed cross-sectional survey data collected from a subset (N = 5520) of the Chi Linh Health and Demographic Surveillance System cohort in Chi Linh district, Vietnam...
July 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28714327/elevated-levels-of-circulating-cell-free-dna-and-neutrophil-proteins-are-associated-with-neonatal-sepsis-and-necrotizing-enterocolitis-in-immature-mice-pigs-and-infants
#9
Duc Ninh Nguyen, Allan Stensballe, Jacqueline Cy Lai, Pingping Jiang, Anders Brunse, Yanqi Li, Jing Sun, Carina Mallard, Tom Skeath, Nicholas D Embleton, Janet E Berrington, Per T Sangild
Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis and specific diagnostic markers are lacking. Circulating cell-free DNA (cfDNA) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. We explored the relation of circulating neutrophil-associated proteins and cfDNA to LOS and/or NEC. Using a clinically relevant preterm pig model of spontaneous LOS and NEC development, we investigated neutrophil-associated proteins and cfDNA in plasma, together with cytokines in gut tissues...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/28710935/measurement-of-isomeric-yield-ratios-of-99m-g-101m-g-102m-g-rh-in-the-nat-pd-%C3%AE-pxn-reactions-with-the-bremsstrahlung-end-point-energies-of-50-70mev
#10
Nguyen Van Do, Pham Duc Khue, Kim Tien Thanh, Nguyen Thi Hien, Guinyun Kim, Kwangsoo Kim, Sung-Gyun Shin, Yong-Uk Kye, Moo-Hyun Cho
The isomeric yield ratios of (99m,g)Rh, (101m,gg)Rh, and (102m,g)Rh isomeric pairs produced from the (nat)Pd(γ,pxn) reactions were measured with the bremsstrahlung end-point energies of 50-, 55-, 60-, 65-, and 70-MeV at the 100MeV electron linac of the Pohang Accelerator Laboratory, Korea. The measurements were carried out with the activation method in combination with off-line γ-ray spectrometry. In order to improve the accuracy of the activity measurements, the separation of the overlapping γ-rays and the necessary corrections for the counting losses were made...
June 20, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28702217/erratum-to-culturally-adaptive-storytelling-intervention-versus-didactic-intervention-to-improve-hypertension-control-in-vietnam-a-cluster-randomized-controlled-feasibility-trial
#11
Hoa L Nguyen, Jeroan J Allison, Duc A Ha, Germán Chiriboga, Ha N Ly, Hanh T Tran, Cuong K Nguyen, Diem M Dang, Ngoc T Phan, Nguyen C Vu, Quang P Nguyen, Robert J Goldberg
[This corrects the article DOI: 10.1186/s40814-017-0136-9.].
2018: Pilot and Feasibility Studies
https://www.readbyqxmd.com/read/28691856/chemical-constituents-from-the-leaves-of-pinus-dalatensis-ferr%C3%A3
#12
Nguyen Hoang Sa, Nguyen Thanh Tam, Nguyen Thi Hoang Anh, Tran Duc Quan, Dao Duc Thien, Dinh Thi Phong, Tran Van Sung, Trinh Thi Thuy
A phytochemical study of n-hexane and ethyl acetate extracts of Pinus dalatensis Ferré leaves led to the isolation of 11 compounds, including one caryolane sesquiterpenoid (1), five labdane diterpenoids (2, 3, 4, 5, 6), one serratane triterpenoid (7), one diacylated flavonoid glucoside (8), one stilbenoid (9) and two sterols (10, 11). The structural characterisation of the isolated compounds was elucidated by spectroscopic data and comparison with the literature report on the chemical constituents from Pinus dalatensis Ferré...
July 10, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28690831/patient-waiting-time-in-the-outpatient-clinic-at-a-central-surgical-hospital-of-vietnam-implications-for-resource-allocation
#13
Tho Dinh Tran, Uy Van Nguyen, Vuong Minh Nong, Bach Xuan Tran
Background: Patient waiting time is considered as a crucial parameter in the assessment of healthcare quality and patients' satisfaction towards healthcare services. Data concerning this has remained limited in Vietnam. Thus, this study aims to assess patient waiting time in the outpatient clinic in Viet Duc Hospital (Hanoi, Vietnam) in order to enable stakeholders to inform evidence-based interventions to improve the quality of healthcare services. Methods: A cross-sectional study was conducted from June 2014 to June 2015 in the outpatient clinic at Viet Duc Hospital...
2017: F1000Research
https://www.readbyqxmd.com/read/28686893/a-relationship-in-adrenal-androgen-levels-between-mothers-and-their-children-from-a-dioxin-exposed-region-in-vietnam
#14
Le Thai Anh, Teruhiko Kido, Seijiro Honma, Ho Dung Manh, Ikue Koike, Yuko Oyama, Hoang Duc Phuc, Rie Okamoto, Hideaki Nakagawa, Shoji F Nakayama, Dang Duc Nhu, Nguyen Hung Minh, Ngo Van Toan, Le Ke Son
Over the past decades, southern Vietnam has been burdened by dioxins from contaminated herbicides sprayed during the Vietnam War. In a previous study, we found that dioxin exposure decreased levels of salivary dehydroepiandrosterone (DHEA), an adrenal androgen, in 3-year-old children. In present study, to assess the relationship between adrenal hormones disruption in lactating mothers and in children, we compared mother-child pairs from dioxin- and nondioxin-contaminated regions. In 2010 and 2011, mother-child pairs from a dioxin hotspot region (n=37) and a non-contaminated region (n=47) were recruited and donated breast milk and serum samples for dioxin and steroid hormones determination...
July 4, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28677027/finding-sex-partners-through-social-media-among-men-who-have-sex-with-men-in-hanoi-vietnam
#15
Aparna Krishnan, Minh Nguyen, Le Minh Giang, Tran Viet Ha, Madhura Bhadra, Sang Minh Nguyen, Viet Duc Vu, Quynh T Nguyen, William C Miller, Vivian F Go
Many men who have sex with men (MSM) in low and middle income countries search for male sexual partners via social media in part due to societal stigma and discrimination, yet little is known about the sexual risk profiles of MSM social media users. This cross-sectional study investigates the prevalence of social media use to find male sex partners in Hanoi, Vietnam and examines associations between social media use and sociodemographic and behavioral characteristics, including levels of internalized, perceived and enacted stigma, high-risk sexual behaviors, and HIV testing...
July 4, 2017: Journal of Community Health
https://www.readbyqxmd.com/read/28670888/molecular-screening-for-epstein-barr-virus-ebv-detection-of-genomic-ebna-1-ebna-2-lmp-1-lmp-2-among-vietnamese-patients-with-nasopharyngeal-brush-samples
#16
Thuan Duc Lao, Dung Huu Nguyen, Trong Minh Nguyen, Thuy Ai Huyen Le
Epstein-Bar virus (EBV) is considered to be intimately associated with development of nasopharyngeal carcinoma (NPC), the most common and high incidence cancer of the head and neck in Asian countries, especially in Vietnam. In this study, we validated associations between highly conserved segments of the EBV genome, including EBNA-1, EBNA-2, LMP-1, LMP-2 and nasopharyngeal cancer, with the aim of applications with non-invasive brush samples for early diagnosis and as a prognostic biomarker. The polymerase chain reaction (PCR) was performed to assess the presence of EBNA-1, EBNA-2, LMP-1, LMP-2 in nasopharyngeal brush samples from Vietnamese NPC patients and non-cancer controls...
June 25, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28669842/human-brucella-melitensis-infections-in-southern-vietnam
#17
EDITORIAL
James I Campbell, Nguyen Phu Huong Lan, Phan Minh Phuong, Le Buu Chau, Trung Pham Duc, Caterina Guzmán-Verri, Nazareth Ruiz-Villalobos, Tam Pham Thi Minh, Pilar M Muñoz Álvaro, Edgardo Moreno, Guy E Thwaites, Maia A Rabaa, Nguyen Van Vinh Chau, Stephen Baker
No abstract text is available yet for this article.
June 29, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28668867/radiosurgery-with-a-rotating-gamma-system-a-very-effective-treatment-for-symptomatic-cerebral-cavernomas
#18
Pham Cam Phuong, Nguyen Duc Luan, Vo Thi Huyen Trang, Steven E Schild, Dirk Rades, Mai Trong Khoa
AIM: To evaluate the value of radiosurgery with a rotating gamma-system (RGS) for cerebral cavernomas. PATIENTS AND METHODS: Seventy-nine patients with symptomatic cerebral cavernomas underwent RGS radiosurgery at the Bach Mai Hospital, Hanoi, Vietnam. Median dose (single fraction) was 20 Gy (range=14-26 Gy). Endpoints included effect on headache, seizures and tumor size. RESULTS: Of 60 patients with headache, 17% had complete response, 82% partial response and 2% stable disease (best response)...
July 2017: Anticancer Research
https://www.readbyqxmd.com/read/28665130/rigidity-strengthening-a-mechanism-for-protein-ligand-binding
#19
Duc D Nguyen, Tian Xiao, Menglun Wang, Guo-Wei Wei
Protein-ligand binding is essential to almost all life processes. The understanding of protein-ligand interactions is fundamentally important to rational drug and protein design. Based on large scale data sets, we show that protein rigidity strengthening or flexibility reduction is a mechanism in protein-ligand binding. Our approach based solely on rigidity is able to unveil a surprisingly apparently long-range contribution of apparently four residue layers to protein-ligand binding, which has ramifications for drug and protein design...
July 12, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28663227/national-healthcare-safety-network-nhsn-dialysis-event-surveillance-report-for-2014
#20
Duc B Nguyen, Alicia Shugart, Christi Lines, Ami B Shah, Jonathan Edwards, Daniel Pollock, Dawn Sievert, Priti R Patel
BACKGROUND AND OBJECTIVES: Persons receiving outpatient hemodialysis are at risk for bloodstream and vascular access infections. The Centers for Disease Control and Prevention conducts surveillance for these infections through the National Healthcare Safety Network. We summarize 2014 data submitted to National Healthcare Safety Network Dialysis Event Surveillance. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Dialysis facilities report three types of dialysis events (bloodstream infections; intravenous antimicrobial starts; and pus, redness, or increased swelling at the hemodialysis vascular access site)...
June 29, 2017: Clinical Journal of the American Society of Nephrology: CJASN
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