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M F Zorzetto, I Martin, Y F R Sancler-Silva, S Zoca, C P Freitas-Dell'Aqua, F O Papa, A A Ramos, J F Nunes, C C M Salgueiro, E Oba
The use of frozen semen for artificial insemination is the main approach utilised for the genetic improvement of most domesticated species. The advantages include lower transportation costs, continuous availability of semen, fewer occurrences of sexually transmitted diseases and the incorporation of desirable genes in a relatively short amount of time. Nevertheless, the use of frozen semen in buffalo herds remains limited due to the loss of sperm quality when buffalo semen is frozen. So, the goal of this study was to evaluate the pre- and post-cryopreservation quality of buffalo semen diluted in three distinct freezing media: Tris-egg yolk, Botu-bov® (BB) and ACP-111®...
May 10, 2017: Andrologia
R Kumar, M Ghosh, N Kumar, A K Balhara, M Gupta, R K Sharma, I Singh
The enormous production potential of buffaloes has never been accomplished due to various reproductive insufficiencies. Among them, post-partum anoestrus, a multifactorial disorder, is predominant but any genetic association is yet to be established. This study focused to identify novel polymorphisms in heat-shock protein 70 (HSP70) gene and its possible association with post-partum anoestrus in Murrah buffaloes. A 579-bp fragment from 5' untranslated region of HSP70 gene was amplified by polymerase chain reaction from blood genomic DNA of 614 animals maintained under similar management conditions...
June 2017: Reproduction in Domestic Animals, Zuchthygiene
Sandeep Dhillod, Dipankar Kar, C S Patil, Subhasish Sahu, Narender Singh
AIM: The aim of the study was to correlate the milk yield of Murrah buffaloes with certain body parts measurements. MATERIALS AND METHODS: A total of 70 lactating Murrah buffaloes were selected from Buffalo Farm, Lala Lajpat Rai University of Veterinary and Animal Science, Hisar and were randomly selected in a range from first to fifth parity. Traits studied were 305 days milk yield (MY), body weight (BW), body length (BL), muzzle width (MW), height at wither (HW), abdominal girth (AG), chest girth (CG), body depth fore, body depth rear, hip bone distance (HBD), pin bone distance (PBD), skin thickness (STK), and tail length (TL)...
January 2017: Veterinary World
Anju Kala, D N Kamra, Avinash Kumar, Neeta Agarwal, L C Chaudhary, C G Joshi
The present study was aimed at understanding a shift in rumen microbiome of buffaloes fed various levels of total digestible nutrients. To understand the process, the metagenomics of rumen microbes, in vivo and in vitro rumen fermentation studies were carried out. Three rumen fistulated adult male Murrah buffaloes were fed three isonitrogenous diets varying in total digestible nutrients (70, 85 and 100% of TDN requirement) in 3X3 switch over design. On dry matter basis, wheat straw/ roughage content were 81, 63 and 51% and that of maize grain was 8, 16 and 21% in three diets respectively...
2017: PloS One
C S Silva, E Silva Filho, A S Matos, A S Schierholt, M R Costa, L C Marques, J S Costa, R L Sales, M R Figueiró, J R F Marques
Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production...
September 16, 2016: Genetics and Molecular Research: GMR
Tingxian Deng, Chunying Pang, Xiaoya Ma, Xingrong Lu, Anqin Duan, Peng Zhu, Xianwei Liang
Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals...
September 27, 2016: Molecular and Cellular Probes
P Singh, A K Verma, S C Gupta, U R Mehra
Thirty growing Murrah buffalo calves (8-12 months of age, 109.85 ± 2.43) were reared in parasite free conditions and randomly divided into three equal groups as per CRD. They were fed on iso-caloric (2.01 ME Mcal/Kg diet) diets containing standard protein (SP) diet at 100 %, 90 % of SP (medium protein, MP) and 80 % of SP (low protein, LP) of the protein requirements (Kearl 1982). After 21 days of feeding, each group was further subdivided into two sub-groups (A & B). Animals in sub-groups 'A' served as non-infected control, while in sub group 'B' were orally infected with Fasciola gigantica metacercarie (mc; 1,000 each)...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Shanshan Li, Ling Li, Qingkun Zeng, Jianxin Liu, Daxi Ren
Understanding the milk protein expression profile in different buffalo breeds plays an important role in improving hybrid selection and determining the effects on milk protein synthesis. The aim of this research is to compare the differences in milk protein content, composition and distribution between River buffalo and their crossbreeds for hybrid screening. Four groups of milk samples that included Nili-Ravi (N), Murrah (M), a Nili-Ravi-Murrah crossbreed (M-N), and a crossbreed of river buffalo with local swamp buffalo (C) were collected...
August 2016: Journal of Dairy Research
Raushan K Singh, A Kumaresan, Shivani Chhillar, Shailendra K Rajak, Utkarsh K Tripathi, Samiksha Nayak, T K Datta, T K Mohanty, R Malhotra
The present study assessed sperm functional characteristics in the frozen-thawed semen of buffalo bulls and estimated their relationship with field fertility. Frozen semen samples from three different freezing operations each from nine Murrah buffalo bulls were used for the assessment of different sperm functions related to fertilizing potential. Bulls were classified into high (n = 2), medium (n = 5), and low (n = 2) fertile based on adjusted field fertility. The sperm functions estimated included membrane integrity using carboxyfluorescein diacetate-propidium iodide, acrosome reaction status using fluorescein isothiocyanate peanut agglutinine, status of apoptosis using Annexin-V, protamine deficiency using Chromomycin A3, membrane stability using Merocyanine 540 and lipid peroxidation status using 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene...
December 2016: Theriogenology
Ana Cláudia de Freitas, Gregório Miguel Ferreira de Camargo, Nedenia Bonvino Stafuzza, Rusbel Raul Aspilcueta-Borquis, Guilherme Costa Venturini, Marina Mortati Dias, Diercles Francisco Cardoso, Humberto Tonhati
This study identified polymorphisms in the DGAT1 gene in Murrah buffaloes and investigated the associations to milk production and quality traits (milk, fat and protein yields and percentages, somatic cell count). Genomic DNA was extracted from hair follicles collected from the tail of 196 females. Three SNPs were identified in DGAT1 gene by sequencing. Statistical analyses were performed to verify the linkage and the association between polymorphisms and traits. The estimated value of r (2) between two SNPs in exon 17 (g...
October 2016: Tropical Animal Health and Production
Jasdeep Singh, C S Mukhopadhyay, Simarjeet Kaur, Puneet Malhotra, R S Sethi, R K Choudhary
In the present study, we used high-throughput sequencing, miRNA-seq, to discover and explore the expression profiles of known and novel miRNAs in TLR ligand-stimulated vis-à-vis non-stimulated (i.e. Control) peripheral blood mononuclear cells (PBMCs) isolated from blood of healthy Murrah buffaloes. Six small RNA (sRNA) libraries were multiplexed in Ion Torrent PI chip and sequenced on Ion Proton System. The reads obtained were aligned to the Bos taurus genome (UMD3.1 assembly), which is phylogenetically closest species to buffalo (Bubalus bubalis)...
2016: PloS One
Sellappan Selvaraju, Binsila B Krishnan, Santhanahalli Siddalingappa Archana, Janivara Parameshwaraiah Ravindra
Insulin like growth factor 1 (IGF1) in the seminal plasma is reported to improve sperm motility by reducing oxidative stress. The present study was conducted to assess the effect of addition of IGF1 on sperm function and protein composition during cryopreservation process. Semen samples were collected from six Murrah buffaloes (2 ejaculates from each animal) and diluted (80 million/ml) in tris egg yolk extender and divided into control, T1, T2 and T3, groups supplemented with 0, 50, 100 and 150 ng of IGF1/mL, respectively...
August 2016: Cryobiology
Kadkao Vongsavan, Praphasri Rirattanapong, Rudee Surarit
Dental caries are a major public health problem worldwide. The aim of this study was to compare the effects of children's follow-on instant powdered cow's milk formula, buffalo milk formula and a chicken-based formula on microhardness of bovine enamel with artificial caries-like lesions. Forty bovine teeth were each placed in acrylic blocks and the enamel surfaces were polished to create flat 5 x 5 millimeter surfaces. The teeth surfaces were then demineralized using 0.1M lactic acid (pH 4.5) to achieve an enamel microhardness of 35-65 Vickers Hardness Numbers (VHN)...
March 2016: Southeast Asian Journal of Tropical Medicine and Public Health
N Haque, M Singh, S A Hossain
The present study was aimed at determining changes in milk yield and composition along with the plasminogen-plasmin system of milk, plasma hormones, and metabolites of buffaloes during hot dry season (air temperature range 39.7 to 44.8 °C) under two different management systems. Buffaloes were divided in two groups of six animals each: control and treatment, where treatment group animals accessed benefit of mist and fan cooling from 9:30 a.m. to 5:00 p.m., while control group animals were devoid of it. Duration of experiment was 6 weeks...
December 2016: International Journal of Biometeorology
A C Freitas, G M F de Camargo, R R Aspilcueta-Borquis, N B Stafuzza, G C Venturini, F Tanamati, N A Hurtado-Lugo, C C Barros, H Tonhati
The study of genes associated with host defense mechanisms, such as the A2M gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. The aim of this study was to identify polymorphisms in the A2M gene in Murrah buffaloes (Bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and protein percentages, and somatic cell count. Hair follicle samples of 136 animals were collected for DNA extraction, and polymorphisms were identified by polymerase chain reactions and sequencing...
April 4, 2016: Genetics and Molecular Research: GMR
G S Meena, V S Raina, A K Gupta, T K Mohanty, M Bhakat, M Abdullah, R Bishist
AIM: To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls. MATERIALS AND METHODS: A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculates from same bull with preputial washing by infusing normal saline (0...
June 2015: Veterinary World
Manoj Kumar, Vikas Vohra, Poonam Ratwan, Jamuna Valsalan, C S Patil, A K Chakravarty
AIM: The present study was performed to investigate the effect of genetic and non-genetic factors affecting milk fat yield and to estimate genetic parameters of monthly test day fat yields (MTDFY) and lactation 305-day fat yield (L305FY) in Murrah buffaloes. MATERIALS AND METHODS: The data on total of 10381 MTDFY records comprising the first four lactations of 470 Murrah buffaloes calved from 1993 to 2014 were assessed. These buffaloes were sired by 75 bulls maintained in an organized farm at ICAR-National Dairy Research Institute, Karnal...
March 2016: Veterinary World
S A Lone, J K Prasad, S K Ghosh, G K Das, B Balamurugan, R Katiyar, M R Verma
AIM: The aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (CLC) treated buffalo spermatozoa. MATERIALS AND METHODS: Semen samples with mass motility of 3+ and greater, collected from Murrah buffalo bulls were utilized. Immediately after collection, four equal groups of semen sample were made. Group I was kept as control and diluted with Tris upto concentration of 60×10(6) sperm/ml, where as Groups II, III, and IV were treated with CLC at 3 mg/120× 10(6) spermatozoa, incubated at 37°C for action of CLC for 10, 15 and 20 min, respectively, and diluted with tris upto concentration of 60×10(6) sperm/ml...
February 2016: Veterinary World
N B Stafuzza, L M Olivatto, B C M Naressi, H Tonhati, M E J Amaral-Trusty
The DRB3 gene is an MHC class II gene that has a high degree of polymorphism with more than 100 alleles described in cattle. This variation contributes to differences among individuals in immune responsiveness and disease resistance. In this study, we searched for allelic variants in exon 2 of the DRB3 gene in 80 river buffaloes of three breeds in Brazil using a PCR-RFLP technique. The PCR product showed genetic polymorphism when digested with RsaI, PstI or HaeIII restriction enzymes. In total, 16 restriction patterns were identified: nine restriction patterns and 16 genotypes were found with RsaI; four restriction patterns and nine genotypes were found with HaeIII; and, three restriction patterns and four genotypes were found with PstI...
March 31, 2016: Genetics and Molecular Research: GMR
Mayukh Ghosh, Meenakshi Gupta, Rajesh Kumar, Sunil Kumar, A K Balhara, Inderjeet Singh
AIM: Objective of the present study was to investigate the relation between antioxidant status and postpartum anestrous (PPA) condition in Murrah buffalo. MATERIALS AND METHODS: Jugular blood samples were collected from two different groups of Murrah buffaloes each group consisting of 20 animals. Group I was of PPA and Group II were of cyclic buffaloes. The animals selected were examined for confirmation for cyclic and acyclic condition (>120 days) after calving by routine transrectal ultrasonography...
October 2015: Veterinary World
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