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Duffy antigen

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https://www.readbyqxmd.com/read/29625249/accurate-typing-of-human-leukocyte-antigen-class-i-genes-by-oxford-nanopore-sequencing
#1
Chang Liu, Fangzhou Xiao, Jessica Hoisington-Lopez, Kathrin Lang, Philipp Quenzel, Brian Duffy, Robi David Mitra
Oxford Nanopore Technologies' MinION has expanded the current DNA sequencing toolkit by delivering long read lengths and extreme portability. The MinION has the potential to enable expedited point-of-care human leukocyte antigen (HLA) typing, an assay routinely used to assess the immunological compatibility between organ donors and recipients, but the platform's high error rate makes it challenging to type alleles with accuracy. Here, we developed and validated accurate typing of HLA by Oxford nanopore (Athlon), a bioinformatic pipeline that i) maps nanopore reads to a database of known HLA alleles, ii) identifies candidate alleles with the highest read coverage at different resolution levels that are represented as branching nodes and leaves of a tree structure, iii) generates consensus sequences by remapping the reads to the candidate alleles, and iv) calls the final diploid genotype by blasting consensus sequences against the reference database...
April 3, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29596498/analyses-of-genome-wide-association-data-cytokines-and-gene-expression-in-african-americans-with-benign-ethnic-neutropenia
#2
Bashira A Charles, Matthew M Hsieh, Adebowale A Adeyemo, Daniel Shriner, Edward Ramos, Kyung Chin, Kshitij Srivastava, Neil A Zakai, Mary Cushman, Leslie A McClure, Virginia Howard, Willy A Flegel, Charles N Rotimi, Griffin P Rodgers
BACKGROUND: Benign ethnic neutropenia (BEN) is a hematologic condition associated with people of African ancestry and specific Middle Eastern ethnic groups. Prior genetic association studies in large population showed that rs2814778 in Duffy Antigen Receptor for Chemokines (DARC) gene, specifically DARC null red cell phenotype, was associated with BEN. However, the mechanism of this red cell phenotype leading to low white cell count remained elusive. METHODS: We conducted an extreme phenotype design genome-wide association study (GWAS), analyzed ~16 million single nucleotide polymorphisms (SNP) in 1,178 African-Americans individuals from the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study and replicated from 819 African-American participants in the Atherosclerosis Risk in Communities (ARIC) study...
2018: PloS One
https://www.readbyqxmd.com/read/29579050/seroepidemiology-of-helminths-and-the-association-with-severe-malaria-among-infants-and-young-children-in-tanzania
#3
Jennifer L Kwan, Amy E Seitz, Michal Fried, Kun-Lin Lee, Simon Metenou, Robert Morrison, Edward Kabyemela, Thomas B Nutman, D Rebecca Prevots, Patrick E Duffy
The disease burden of Wuchereria bancrofti and Plasmodium falciparum malaria is high, particularly in Africa, and co-infection is common. However, the effects of filarial infection on the risk of severe malaria are unknown. We used the remaining serum samples from a large cohort study in Muheza, Tanzania to describe vector-borne filarial sero-reactivity among young children and to identify associations between exposure to filarial parasites and subsequent severe malaria infections. We identified positive filarial antibody responses (as well as positive antibody responses to Strongyloides stercoralis) among infants as young as six months...
March 26, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29530446/plasmodium-vivax-infections-of-duffy-negative-erythrocytes-historically-undetected-or-a-recent-adaptation
#4
REVIEW
Karthigayan Gunalan, Amadou Niangaly, Mahamadou A Thera, Ogobara K Doumbo, Louis H Miller
Plasmodium vivax is the main cause of malarial disease in Asia and South America. Plasmodium vivax infection was thought to be absent in African populations who are Duffy blood group antigen negative (Duffy-negative). However, many cases of P. vivax infection have recently been observed in Duffy-negative Africans. This raises the question: were P. vivax infections in Duffy-negative populations previously missed or has P. vivax adapted to infect Duffy-negative populations? This review focuses on recent P. vivax findings in Africa and reports views on the parasite ligands that may play a role in Duffy-negative P...
March 9, 2018: Trends in Parasitology
https://www.readbyqxmd.com/read/29529020/the-plasmodium-falciparum-transcriptome-in-severe-malaria-reveals-altered-expression-of-genes-involved-in-important-processes-including-surface-antigen-encoding-var-genes
#5
Gerry Q Tonkin-Hill, Leily Trianty, Rintis Noviyanti, Hanh H T Nguyen, Boni F Sebayang, Daniel A Lampah, Jutta Marfurt, Simon A Cobbold, Janavi S Rambhatla, Malcolm J McConville, Stephen J Rogerson, Graham V Brown, Karen P Day, Ric N Price, Nicholas M Anstey, Anthony T Papenfuss, Michael F Duffy
Within the human host, the malaria parasite Plasmodium falciparum is exposed to multiple selection pressures. The host environment changes dramatically in severe malaria, but the extent to which the parasite responds to-or is selected by-this environment remains unclear. From previous studies, the parasites that cause severe malaria appear to increase expression of a restricted but poorly defined subset of the PfEMP1 variant, surface antigens. PfEMP1s are major targets of protective immunity. Here, we used RNA sequencing (RNAseq) to analyse gene expression in 44 parasite isolates that caused severe and uncomplicated malaria in Papuan patients...
March 12, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29523137/antibody-levels-to-recombinant-var2csa-domains-vary-with-plasmodium-falciparum-parasitaemia-gestational-age-and-gravidity-but-do-not-predict-pregnancy-outcomes
#6
Michal Fried, Jonathan D Kurtis, Bruce Swihart, Robert Morrison, Sunthorn Pond-Tor, Amadou Barry, Youssoufa Sidibe, Sekouba Keita, Almahamoudou Mahamar, Naissem Andemel, Oumar Attaher, Adama B Dembele, Kadidia B Cisse, Bacary S Diarra, Moussa B Kanoute, David L Narum, Alassane Dicko, Patrick E Duffy
BACKGROUND: Maternal malaria is a tropical scourge associated with poor pregnancy outcomes. Women become resistant to Plasmodium falciparum pregnancy malaria as they acquire antibodies to the variant surface antigen VAR2CSA, a leading vaccine candidate. Because malaria infection may increase VAR2CSA antibody levels and thereby confound analyses of immune protection, gravidity-dependent changes in antibody levels during and after infection, and the effect of VAR2CSA antibodies on pregnancy outcomes were evaluated...
March 9, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29504337/red-cell-alloimmunization-in-multitransfused-thalassaemia-major-patients
#7
Shazia Moeen, Nazish Farooq, Romana Irshad, Muhammad Ashfaq, Umer Farooq, Muhammad Idris
BACKGROUND: Lifelong transfusions are life savers for thalassaemia patients but are associated with many complications. Alloimmunization is a major problem for blood banks. Antigens of foreign red blood cells induce the formation of antibodies in patients suffering from thalassaemia. The purpose of this study was to examine the frequency of red cell alloantibodies and to express the type of these antibodies in thalassaemia patients. METHODS: Patients that have received multiple transfusions were included in this study...
January 2018: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29434602/identification-of-a-novel-cd8-t-cell-epitope-derived-from-plasmodium-berghei-protective-liver-stage-antigen
#8
Alexander Pichugin, Stasya Zarling, Leah Perazzo, Patrick Emmet Duffy, Hidde Lolke Ploegh, Urszula Krzych
We recently identified novel Plasmodium berghei (Pb) liver stage (LS) genes that as DNA vaccines significantly reduce Pb LS parasite burden (LPB) in C57Bl/6 (B6) mice through a mechanism mediated, in part, by CD8 T cells. In this study, we sought to determine fine antigen (Ag) specificities of CD8 T cells that target LS malaria parasites. Guided by algorithms for predicting MHC class I-restricted epitopes, we ranked sequences of 32 Pb LS Ags and selected ~400 peptides restricted by mouse H-2Kb and H-2Db alleles for analysis in the high-throughput method of caged MHC class I-tetramer technology...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29421483/effect-of-suckler-cow-vaccination-against-glycoprotein-e-ge-negative-bovine-herpesvirus-type-1-bohv-1-on-passive-immunity-and-physiological-response-to-subsequent-bovine-respiratory-disease-vaccination-of-their-progeny
#9
Bernadette Earley, Katie Tiernan, Catherine Duffy, Amanda Dunn, Sinead Waters, Steven Morrison, Mark McGee
The study objectives were: 1) to characterise the development of immunocompetence in beef suckler calves from birth to three months of age, and 2) to trace glycoprotein E (gE)-negative bovine herpesvirus type 1 (BoHV-1) antibodies from dam to calf and subsequent vaccination against pneumonia. Thirty multiparous beef suckler, spring-calving cows, consisting of two genotypes were involved; Limousin×Friesian (LF) and Charolais×Limousin (CL). Cows were immunised against the inactivated antigen strain of BoHV-1 (gE- (IBR marker vaccine) at day -84 and received a booster at day -56 relative to the expected calving date (d 0)...
January 10, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29378145/assessment-of-common-red-blood-cell-pretreatments-to-yield-an-accurate-serologic-antigen-phenotype-compared-with-genotypepredicted-phenotype
#10
Trina Horn, Janis Hamilton, Joanne Kosanke, Virginia W Hare, Wendy Kluver, Wendy Beres, Sandra Nance, Margaret A Keller
For patients requiring multiple transfusions and patients with positive direct antiglobulin tests (DATs), an extended red blood cell (RBC) phenotype can provide valuable information and help to determine the risk of forming alloantibodies. In some instances, the phenotype may be used for prophylactic matching. Phenotyping in this patient population is often hindered by the presence of circulating donor cells and/or by a positive DAT. Several methods, such as EDTA glycine acid (EGA) treatment to remove IgG, hypotonic saline wash to separate autologous RBCs, or reticulocyte separation, are often used in these situations to isolate patient RBCs for serologic phenotyping...
December 2017: Immunohematology
https://www.readbyqxmd.com/read/29365318/recipient-priming-to-one-rbc-alloantigen-directly-enhances-subsequent-alloimmunization-in-mice
#11
Seema R Patel, Ashley Bennett, Kathryn Girard-Pierce, Cheryl L Maier, Satheesh Chonat, Connie M Arthur, Patricia E Zerra, Amanda Mener, Sean R Stowell
Individuals that become immunized to red blood cell (RBC) alloantigens can experience an increased rate of antibody formation to additional RBC alloantigens following subsequent transfusion. Despite this, how an immune response to one RBC immunogen may impact subsequent alloimmunization to a completely different RBC alloantigen remains unknown. Our studies demonstrate that Kell blood group antigen (KEL) RBC transfusion in the presence of inflammation induced by poly (I:C) (PIC) not only enhances anti-KEL antibody production through a CD4+ T-cell-dependent process but also directly facilitates anti-HOD antibody formation following subsequent exposure to the disparate HOD (hen egg lysozyme, ovalbumin, fused to human blood group antigen Duffy b) antigen...
January 23, 2018: Blood Advances
https://www.readbyqxmd.com/read/29358275/erythrocyte-saturation-with-igg-is-required-for-inducing-antibody-mediated-immune-suppression-and-impacts-both-erythrocyte-clearance-and-antigen-modulation-mechanisms
#12
Yoelys Cruz-Leal, Danielle Marjoram, Alan H Lazarus
Anti-D prevents hemolytic disease of the fetus and newborn, and this mechanism has been referred to as Ab-mediated immune suppression (AMIS). Anti-D, as well as other polyclonal AMIS-inducing Abs, most often induce both epitope masking and erythrocyte clearance mechanisms. We have previously observed that some Abs that successfully induce AMIS effects could be split into those that mediate epitope masking versus those that induce erythrocyte clearance, allowing the ability to analyze these mechanisms separately...
February 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29341885/deletion-of-the-duffy-antigen-receptor-for-chemokines-darc-promotes-insulin-resistance-and-adipose-tissue-inflammation-during-high-fat-feeding
#13
Tyler W Benson, Daniel S Weintraub, Matthew Crowe, Nicole K H Yiew, Orishebawo Popoola, Ajay Pillai, Joel Joseph, Krystal Archer, Charlotte Greenway, Tapan K Chatterjee, James Mintz, David W Stepp, Brian K Stansfield, Weiqin Chen, Julia Brittain, Vladimir Y Bogdanov, Yan Gao, James G Wilson, Yaoliang Tang, Ha Won Kim, Neal L Weintraub
OBJECTIVE: Inflammation in adipose tissues in obesity promotes insulin resistance and metabolic disease. The Duffy antigen receptor for chemokines (DARC) is a promiscuous non-signaling receptor expressed on erythrocytes and other cell types that modulates tissue inflammation by binding chemokines such as monocyte chemoattractant protein-1 (MCP-1) and by acting as a chemokine reservoir. DARC allelic variants are common in humans, but the role of DARC in modulating obesity-related metabolic disease is unknown...
January 16, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29330014/frequency-of-red-blood-cell-genotypes-in-multi-transfused-patients-and-blood-donors-from-minas-gerais-southeast-brazil
#14
Vitor Mendonça Alves, Fernanda Bernadelli De Vito, Paulo Roberto Juliano Martins, Sheila Soares Silva, Lilian Castilho, Helio Moraes-Souza
BACKGROUND AND OBJECTIVES: The frequency of red blood cell (RBC) antigens in Brazil varies due to differences in the ethnic groups in different regions; however, these studies have not been performed in Minas Gerais, where African admixture is more prevalent in comparison with other states. Due to these facts, this study aimed to determine the frequency of RBC genotypes on Rh, Kell, Duffy and Kidd systems in blood donors and multi-transfused patients from Minas Gerais, Southeast Brazil...
February 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29281708/protein-protein-conjugate-nanoparticles-for-malaria-antigen-delivery-and-enhanced-immunogenicity
#15
Puthupparampil V Scaria, Beth Chen, Christopher G Rowe, David S Jones, Emma Barnafo, Elizabeth R Fischer, Charles Anderson, Nicholas J MacDonald, Lynn Lambert, Kelly M Rausch, David L Narum, Patrick E Duffy
Chemical conjugation of polysaccharide to carrier proteins has been a successful strategy to generate potent vaccines against bacterial pathogens. We developed a similar approach for poorly immunogenic malaria protein antigens. Our lead candidates in clinical trials are the malaria transmission blocking vaccine antigens, Pfs25 and Pfs230D1, individually conjugated to the carrier protein Exoprotein A (EPA) through thioether chemistry. These conjugates form nanoparticles that show enhanced immunogenicity compared to unconjugated antigens...
2017: PloS One
https://www.readbyqxmd.com/read/29281179/a-high-parasite-density-environment-induces-transcriptional-changes-and-cell-death-in-plasmodium-falciparum-blood-stages
#16
Evelyn S Chou, Sabia Z Abidi, Marian Teye, Aleksandra Leliwa-Sytek, Thomas S Rask, Simon A Cobbold, Gerry Q Tonkin-Hill, Krishanthi S Subramaniam, Anna E Sexton, Darren J Creek, Johanna P Daily, Michael F Duffy, Karen P Day
Transient regulation of Plasmodium numbers below the density that induces fever has been observed in chronic malaria infections in humans. This species transcending control cannot be explained by immunity alone. Using an in vitro system we have observed density dependent regulation of malaria population size as a mechanism to possibly explain these in vivo observations. Specifically, Plasmodium falciparum blood stages from a high but not low-density environment exhibited unique phenotypic changes during the late trophozoite (LT) and schizont stages of the intraerythrocytic cycle...
March 2018: FEBS Journal
https://www.readbyqxmd.com/read/29259245/atp-degrading-enpp1-is-required-for-survival-or-persistence-of-long-lived-plasma-cells
#17
Hongsheng Wang, Ines Gonzalez-Garcia, Javier Traba, Shweta Jain, Solomon Conteh, Dong-Mi Shin, Chenfeng Qi, Yuanyuan Gao, Jiafang Sun, Sungyun Kang, Sadia Abbasi, Zohreh Naghashfar, Jeongheon Yoon, Wendy DuBois, Alexander L Kovalchuk, Michael N Sack, Patrick Duffy, Herbert C Morse
Survival of antibody-secreting plasma cells (PCs) is vital for sustained antibody production. However, it remains poorly understood how long-lived PCs (LLPCs) are generated and maintained. Here we report that ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) is preferentially upregulated in bone marrow LLPCs compared with their splenic short-lived counterparts (SLPCs). We studied ENPP1-deficient mice (Enpp1-/- ) to determine how the enzyme affects PC biology. Although Enpp1-/- mice generated normal levels of germinal center B cells and plasmablasts in periphery, they produced significantly reduced numbers of LLPCs following immunization with T-dependent antigens or infection with plasmodium C...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29194667/evaluation-of-the-impact-of-banking-umbilical-cord-blood-units-with-high-cell-dose-for-ethnically-diverse-patients
#18
Gretta Stritesky, Kimberly Wadsworth, Merry Duffy, Kelly Buck, Jason Dehn
BACKGROUND: Umbilical cord blood units provide an important stem cell source for transplantation, particularly for patients of ethnic diversity who may not have suitably matched available, adult-unrelated donors. However, with the cost of cord blood unit acquisition from public banks significantly higher than that for adult-unrelated donors, attention is focused on decreasing cost yet still providing cord blood units to patients in need. Historical practices of banking units with low total nucleated cell counts, including units with approximately 90 × 107 total nucleated cells, indicates that most banked cord blood units have much lower total nucleated cell counts than are required for transplant...
February 2018: Transfusion
https://www.readbyqxmd.com/read/29154744/generation-and-characterization-of-a-novel-candidate-gene-therapy-and-vaccination-vector-based-on-human-species-d-adenovirus-type-56
#19
Margaret R Duffy, Julio Alonso-Padilla, Lijo John, Naresh Chandra, Selina Khan, Monika Z Ballmann, Agnieszka Lipiec, Evert Heemskerk, Jerome Custers, Niklas Arnberg, Menzo Havenga, Andrew H Baker, Angelique Lemckert
The vectorization of rare human adenovirus (HAdV) types will widen our knowledge of this family and their interaction with cells, tissues and organs. In this study we focus on HAdV-56, a member of human Ad species D, and create ease-of-use cloning systems to generate recombinant HAdV-56 vectors carrying foreign genes. We present in vitro transduction profiles for HAdV-56 in direct comparison to the most commonly used HAdV-5-based vector. In vivo characterizations demonstrate that when it is delivered intravenously (i...
January 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29062250/hyperbilirubinemia-due-to-minor-blood-group-anti-e-incompatibility-in-a-newborn-a-case-report
#20
Murat Özcan, Selin Sevinç, Vildan Boz Erkan, Yüksel Yurdugül, S Ümit Sarıcı
In addition to Rh and ABO incompatibilities subgroup incompatibilities may rarely play a role among the causes of hemolytic anemia and indirect hyperbilirubinemia in newborns. The most common minor blood group antigens that cause blood incompatibility between the mother and baby are C, c, E, e, Kell, Duffy, Diego, Kidd and MNSs antigens. In this article, a newborn in whom hyperbilirubinemia due to anti-E minor blood group incompatibility developed and was treated with phototherapy succesfully is presented and minor blood group incompatibilities due to anti-E are reviewed...
September 2017: Türk Pediatri Arşivi
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