keyword
MENU ▼
Read by QxMD icon Read
search

Protiens

keyword
https://www.readbyqxmd.com/read/27822074/diagnosis-of-exercise-induced-anaphylaxis-current-insights
#1
REVIEW
Valerio Pravettoni, Cristoforo Incorvaia
Exercise-induced anaphylaxis (EIAn) is defined as the occurrence of anaphylactic symptoms (skin, respiratory, gastrointestinal, and cardiovascular symptoms) after physical activity. In about a third of cases, cofactors, such as food intake, temperature (warm or cold), and drugs (especially nonsteroidal anti-inflammatory drugs) can be identified. When the associated cofactor is food ingestion, the correct diagnosis is food-dependent EIAn (FDEIAn). The literature describes numerous reports of FDEIAn after intake of very different foods, from vegetables and nuts to meats and seafood...
2016: Journal of Asthma and Allergy
https://www.readbyqxmd.com/read/27784063/a-novel-method-combining-vitreous-aspiration-and-intravitreal-aav2-8-injection-results-in-retina-wide-transduction-in-adult-mice
#2
Romain Da Costa, Carsten Röger, Jasmin Segelken, Maya Barben, Christian Grimm, John Neidhardt
Purpose: Gene therapies to treat eye disorders have been extensively studied in the past 20 years. Frequently, adeno-associated viruses were applied to the subretinal or intravitreal space of the eye to transduce retinal cells with nucleotide sequences of therapeutic potential. In this study we describe a novel intravitreal injection procedure that leads to a reproducible adeno-associated virus (AAV)2/8-mediated transduction of more than 70% of the retina. Methods: Prior to a single intravitreal injection of a enhanced green fluorescent protien (GFP)-expressing viral suspension, we performed an aspiration of vitreous tissue from wild-type C57Bl/6J mice...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27666726/rab5a%C3%A2-mediated-autophagy-regulates-the-phenotype-and-behavior-of-vascular-smooth-muscle-cells
#3
Jin-Yun Tan, Luo-Qi Jia, Wei-Hao Shi, Qing He, Lei Zhu, Bo Yu
Rab5a, a key member of the Rab family of GTPases, was determined to be a regulator of vascular smooth muscle cell (VSMC) proliferation and migration. However, the exact regulatory mechanism remains unclear. As Rab5a has been shown to be associated with autophagy, which is essential for the conversion of VSMCs from a contractile to a synthetic phenotype in order to prevent cell death due to oxidative stress. The present study hypothesized that autophagy may be responsible for the proliferation and migration of VSMCs via the Rab5a protein...
November 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27596929/a-clinical-biological-and-biomaterials-perspective-into-tendon-injuries-and-regeneration
#4
Grace Walden, Xin Liao, Simon Donell, Mike J Raxworthy, Graham P Riley, Aram Saeed
Tendon injury is common and debilitating, and it is associated with long-term pain and ineffective healing. It is estimated to afflict 25% of the adult population and is often a career-ending disease in athletes and racehorses. Tendon injury is associated with high morbidity, pain, and long-term suffering for the patient. Due to the low cellularity and vascularity of tendon tissue, once damage has occurred, the repair process is slow and inefficient, resulting in mechanically, structurally, and functionally inferior tissue...
September 30, 2016: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/27194859/immunohistochemical-expression-of-p53-and-its-clinicopathological-correlation-with-modified-anneroth-s-histological-grading-system
#5
Kajal V Dave, Monali Chalishazar, Vishal R Dave, Pritam Panja, Manisha Singh, Tapan G Modi
INTRODUCTION AND OBJECTIVES: Oral squamous cell carcinoma (OSCC) is an epithelial neoplasm generally beginning as focal overgrowth of altered stem cells near the basement membrane, moving upward and laterally, replacing the normal epithelium. Histopathological grading has been used for many decades in an attempt to predict the clinical behavior of oral squamous cell carcinoma. In the present study, Forty biopsies were studied for histological grading and p53 expression. The p53 expression was studied in relation to clinical parameters such as age, sex of patient and site of tumors...
January 2016: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/27050366/cdc2-like-kinase-2-is-a-key-regulator-of-the-cell-cycle-via-foxo3a-p27-in-glioblastoma
#6
Soon Young Park, Yuji Piao, Craig Thomas, Gregory N Fuller, John F de Groot
Cdc2-like kinase 2 (CLK2) is known as a regulator of RNA splicing that ultimately controls multiple physiological processes. However, the function of CLK2 in glioblastoma progression has not been described. Reverse-phase protein array (RPPA) was performed to identify proteins differentially expressed in CLK2 knockdown cells compared to controls. The RPPA results indicated that CLK2 knockdown influenced the expression of survival-, proliferation-, and cell cycle-related proteins in GSCs. Thus, knockdown of CLK2 expression arrested the cell cycle at the G1 and S checkpoints in multiple GSC lines...
May 3, 2016: Oncotarget
https://www.readbyqxmd.com/read/26648565/knockdown-of-nogoa-prevents-mpp-%C3%A2-induced-neurotoxicity-in-pc12-cells-via-the-mtor-stat3-signaling-pathway
#7
Jianbin Zhong, Xie Li, Limei Wan, Zhibang Chen, Simin Zhong, Songhua Xiao, Zhengwen Yan
NogoA is a myelin‑associated protein, which is important in the inhibition of axonal fiber growth and in regeneration following injury of the mammalian central nervous system. A previous study suggested that NogoA may be key in the process of Parkinson's disease (PD), which is the second most common chronic neurodegenerative disorder worldwide. The regulatory mechanism underlying the effect of NogoA on the process of PD remains to be fully elucidated. The present study aimed to investigate the effect and underlying mechanism of NogoA on cellular viability, apoptosis and autophagy induced by 1-methyl-4-phenylpyridinium (MPP+) in PC12 cells, a commonly used in vitro PD model...
February 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/26364481/characterization-of-rice-oryza-sativa-l-genotypes-on-the-basis-of-morpho-physiological-and-biochemical-traits-grown-under-aerobic-situation-in-rainfed-ecosystem
#8
Santosh Kumar, Sharad Kumar Dwivedi, S S Singh, Sanjeev Kumar, R K Sundaram, Shivani, A K Mall
The objective of the present study was to examine the effect of aerobic situation on yield, physiological and biochemical traits of advanced breeding lines of rice. Experiment was conducted with two set of rice genotypes under two water regimes (aerobic and irrigated), during three consecutive wet seasons 2010-2012. Significant decrease in yield was observed in rice genotypes grown under aerobic situation as compared to the irrigated ones. Promising rice genotypes having the ability to maintain high plant biomass, harvest index, early vegetative vigour, improved physiological and biochemical traits in terms of relative water content (RWC), leaf area index (LAI), total soluble sugar, starch, protien and proline content help to sustain higher grain yield under aerobic situation...
July 2015: Journal of Environmental Biology
https://www.readbyqxmd.com/read/26355389/igg4-related-tubulointerstitial-nephritis-a-prospective-analysis
#9
Ritambhra Nada, Raja Ramachandran, Ashwani Kumar, Manish Rathi, Amit Rawat, Kusum Joshi, Harbir Singh Kohli, Krishan Lal Gupta
AIMS: Immunoglobulin-G4 (IgG4)-related tubulo-interstitial nephritis (IgG4TIN) could be the first presentation of IgG4-related systemic disease. Most of the data is from the West or Japan and retrospective, with good patient outcome. METHODS: This study was carried out from April 2011 to July 2013. We report a prospective follow-up of 11 patients who presented with renal dysfunction and had histological diagnosis of IgG4TIN followed for a minimum period of 1 year or until end-stage renal disease...
July 2016: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/26336744/-modified-protiens-and-their-association-with-psychoemotional-factors-in-patients-with-various-degrees-blood-pressure
#10
O Yu Bastrikov
Studied the level of induced oxidative modified serum proteins, depending on the stage and extent of arterial hypertension. With the progression of the severity of hypertension found a significant increase in fragmentation of oxidized protein products after their spontaneous and metal-catalyzed oxidation at a wavelength of 270 nm. The study established the association of oxidative modification of proteins and studied psychological parameters in patients with arterial hypertension. The nature of stress changes, their biochemical support is essential for the understanding of the mechanism of influence of stress on the development and course of systemic diseases, develop integration approach to the prevention of psychosomatic disorders...
April 2015: Rossiĭskii Fiziologicheskiĭ Zhurnal Imeni I.M. Sechenova
https://www.readbyqxmd.com/read/26247845/selective-multifaceted-e3-ubiquitin-ligases-barricade-extreme-defense-potential-therapeutic-targets-for-neurodegeneration-and-ageing
#11
REVIEW
Arun Upadhyay, Ayeman Amanullah, Deepak Chhangani, Ribhav Mishra, Amit Mishra
Efficient and regular performance of Ubiquitin Proteasome System and Autophagy continuously eliminate deleterious accumulation of nonnative protiens. In cellular quality control system, E3 ubiquitin ligases are significant employees for defense mechanism against abnormal toxic proteins. Few findings indicate that lack of functions of E3 ubiquitin ligases can be a causative factor of neurodevelopmental disorders, neurodegeneration, cancer and ageing. However, the detailed molecular pathomechanism implying E3 ubiquitin ligases in cellular functions in multifactorial disease conditions are not well understood...
November 2015: Ageing Research Reviews
https://www.readbyqxmd.com/read/26198082/streptomycin-affinity-depends-on-13-amino-acids-forming-a-loop-in-homology-modelled-ribosomal-s12-protein-rpsl-gene-of-lysinibacillus-sphaericus-dsls5-associated-with-marine-sponge-tedania-anhelans
#12
Balasubramanian Suriyanarayanan, Praveena Pothuraju Lakshmi, Ramachandran Sarojini Santhosh, Kandasamy Dhevendaran, Balakrishnan Priya, Shivaani Krishna
Streptomycin, an antibiotic used against microbial infections, inhibits the protein synthesis by binding to ribosomal protein S12, encoded by rpsL12 gene, and associated mutations cause streptomycin resistance. A streptomycin resistant, Lysinibacillus sphaericus DSLS5 (MIC >300 µg/mL for streptomycin), was isolated from a marine sponge (Tedania anhelans). The characterisation of rpsL12 gene showed a region having similarity to long terminal repeat sequences of murine lukemia virus which added 13 amino acids for loop formation in RpsL12; in addition, a K56R mutation which corresponds to K43R mutation present in streptomycin-resistant Escherichia coli is also present...
June 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/25941724/-capacity-of-hyperthermophilic-crenarchaeota-for-decomposition-of-refractory-protiens-%C3%AE-and-%C3%AE-keratins
#13
S Kh Bidzhieva, K S Derbikova, I V Kublanov, E A Bonch-Osmolovskaya
Anaerobic thermophilic archaea of the genera Thermogladius and Desulfurococcus capable of a- and P3-keratin decomposition were isolated from hot springs of Kamchatka and Kunashir Island. For two of them (strains 2355k and 3008g), the presence of high-molecular mass, cell-bound endopeptidases active against nonhydrolyzed and partially hydrolyzed proteins at high values of temperature and pH was shown. Capacity for β-keratin decomposition was also found in collection strains (type strains of Desulfurococcus amylolyticus subsp...
November 2014: Mikrobiologiia
https://www.readbyqxmd.com/read/25888864/novel-pegylated-silver-coated-carbon-nanotubes-kill-salmonella-but-they-are-non-toxic-to-eukaryotic-cells
#14
Atul A Chaudhari, Shanese L Jasper, Ejovwoke Dosunmu, Michael E Miller, Robert D Arnold, Shree R Singh, Shreekumar Pillai
BACKGROUND: Resistance of food borne pathogens such as Salmonella to existing antibiotics is of grave concern. Silver coated single walled carbon nanotubes (SWCNTs-Ag) have broad-spectrum antibacterial activity and may be a good treatment alternative. However, toxicity to human cells due to their physico-chemical properties is a serious public health concern. Although pegylation is commonly used to reduce metal nanoparticle toxicity, SWCNTs-Ag have not been pegylated as yet, and the effect of pegylation of SWCNTs-Ag on their anti-bacterial activity and cell cytotoxicity remains to be studied...
2015: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/25736504/affinity-binding-of-antibodies-to-supermacroporous-cryogel-adsorbents-with-immobilized-protein-a-for-removal-of-anthrax-toxin-protective-antigen
#15
Ganesh C Ingavle, Les W J Baillie, Yishan Zheng, Elzbieta K Lis, Irina N Savina, Carol A Howell, Sergey V Mikhalovsky, Susan R Sandeman
Polymeric cryogels are efficient carriers for the immobilization of biomolecules because of their unique macroporous structure, permeability, mechanical stability and different surface chemical functionalities. The aim of the study was to demonstrate the potential use of macroporous monolithic cryogels for biotoxin removal using anthrax toxin protective antigen (PA), the central cell-binding component of the anthrax exotoxins, and covalent immobilization of monoclonal antibodies. The affinity ligand (protein A) was chemically coupled to the reactive hydroxyl and epoxy-derivatized monolithic cryogels and the binding efficiencies of protein A, monoclonal antibodies to the cryogel column were determined...
May 2015: Biomaterials
https://www.readbyqxmd.com/read/25598076/development-of-biomimetic-tilapia-collagen-nanofibers-for-skin-regeneration-through-inducing-keratinocytes-differentiation-and-collagen-synthesis-of-dermal-fibroblasts
#16
Tian Zhou, Nanping Wang, Yang Xue, Tingting Ding, Xin Liu, Xiumei Mo, Jiao Sun
In this study, tilapia skin collagen sponge and electrospun nanofibers were developed for wound dressing. The collagen sponge was composed of at least two α-peptides, and its denaturation temperature was 44.99 °C. It did not change the number of spleen-derived lymphocytes in BALB/c mice, the ratio of CD4+/CD8+ lymphocytes, and the level of IgG or IgM in Sprague-Dawley rat. The contact angle, tensile strength, and weight loss temperature of collagen nanofibers were 21.2°, 6.72±0.44 MPa, and 300 °C, respectively...
February 11, 2015: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/25571634/-the-cardioprotective-effects-of-ischemic-postconditioning-on-myocardial-interstitium-following-ischemic-reperfusion-in-rats
#17
Yan-Zhen Lu, Jia Wang, Cui-Ying Zhang, Juan Song, Bao-Hong Li, Xiao-Liang Song
OBJECTIVE: To investigate the effects of ischemic postconditioning (IPTC) on the changes of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) protein and mRNA levels in rat heart subjected to ischemia/reperfusion, and explore the mechanism by which IPTC protects myocardial interstitium following ischemic/reperfusion (I/R). METHODS: Twenty four healthy male SD rats were randomly divided into 3 groups (n = 8): sham control (SC) group, I/R group and IPTC group...
September 2014: Chinese Journal of Applied Physiology
https://www.readbyqxmd.com/read/25543045/il-33-promotes-mouse-keratinocyte-derived-chemokine-an-il-8-homologue-expression-in-airway-smooth-muscle-cells-in-ovalbumin-sensitized-mice
#18
Wei Wu, Yuzhu Xu, Xinliang He, Yan Lu, Yali Guo, Zhuoran Yin, Jungang Xie, Jianping Zhao
BACKGROUND: Although it is recognized that IL-33 plays a key role in the onset of asthma, it is currently unclear whether IL-33 acts on any other target cells besides mast cells and Th2 cells in asthma. We investigated that whether airway smooth muscle cells (ASMCs) could contribute to asthma via stimulation with IL-33. METHODS: To create a mouse model of acute asthma, murine ASMCs were isolated and cultured in vitro with IL-33. The ASMCs were divided into two groups, ASMCs from normal mice and ASMCs from ovalbumin-sensitized mice...
December 2014: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/25418884/functionally-deficient-mesenchymal-stem-cells-reside-in-the-bone-marrow-niche-with-m2-macrophages-and-amyloid-%C3%AE-protein-adjacent-to-loose-total-joint-implants
#19
Bryan S Margulies, Sean D DeBoyace, Adrienne M Parsons, Connor G Policastro, Jessica S S Ee, Timothy S Damron
We sought to demonstrate whether there is a difference in the local mesenchymal stem cells (MSC) niche obtained from patients undergoing their first total joint replacement surgery versus those patients undergoing a revision surgery for an failing total joint implant. Bone marrow aspirates collected from patients undergoing revision total joint arthroplasty were observed to be less clonal and the expression of PDGFRα, CD51, ALCAM, endoglin, CXCL12, nestin, and nucleostemin were decreased. Revision MSC were also less able to commit to an osteoblast-lineage or an adipocyte-lineage...
May 2015: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/25329354/slit2-exerts-anti-inflammatory-actions-in-human-placenta-and-is-decreased-with-maternal-obesity
#20
Ratana Lim, Martha Lappas
PROBLEM: Obese pregnancies are characterised by increased inflammation. Members of the Slit/Roundabout (Robo) family are key regulators of the inflammatory response. The aim of this study was to determine the effect of (i) pre-existing maternal obesity on Slit-Robo expression in human placenta and (ii) Slit2 knockdown by siRNA in primary trophoblast cells on markers of inflammation. METHOD OF STUDY: The expression of Slit-Robo protiens was assessed in human placenta from lean (n = 15) and obese (n = 16) patients by qRT-PCR and Western blotting...
January 2015: American Journal of Reproductive Immunology: AJRI
keyword
keyword
59130
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"