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Jin-Yun Tan, Luo-Qi Jia, Wei-Hao Shi, Qing He, Lei Zhu, Bo Yu
Rab5a, a key member of the Rab family of GTPases, was determined to be a regulator of vascular smooth muscle cell (VSMC) proliferation and migration. However, the exact regulatory mechanism remains unclear. As Rab5a has been shown to be associated with autophagy, which is essential for the conversion of VSMCs from a contractile to a synthetic phenotype in order to prevent cell death due to oxidative stress. The present study hypothesized that autophagy may be responsible for the proliferation and migration of VSMCs via the Rab5a protein...
September 26, 2016: Molecular Medicine Reports
Grace Walden, Xin Liao, Simon Donell, Mike J Raxworthy, Graham P Riley, Aram Saeed
Tendon injury is common and debilitating, and it is associated with long-term pain and ineffective healing. It is estimated to afflict 25% of the adult population and is often a career-ending disease in athletes and racehorses. Tendon injury is associated with high morbidity, pain, and long-term suffering for the patient. Due to the low cellularity and vascularity of tendon tissue, once damage has occurred, the repair process is slow and inefficient, resulting in mechanically, structurally, and functionally inferior tissue...
September 30, 2016: Tissue Engineering. Part B, Reviews
Kajal V Dave, Monali Chalishazar, Vishal R Dave, Pritam Panja, Manisha Singh, Tapan G Modi
INTRODUCTION AND OBJECTIVES: Oral squamous cell carcinoma (OSCC) is an epithelial neoplasm generally beginning as focal overgrowth of altered stem cells near the basement membrane, moving upward and laterally, replacing the normal epithelium. Histopathological grading has been used for many decades in an attempt to predict the clinical behavior of oral squamous cell carcinoma. In the present study, Forty biopsies were studied for histological grading and p53 expression. The p53 expression was studied in relation to clinical parameters such as age, sex of patient and site of tumors...
January 2016: Journal of Oral and Maxillofacial Pathology: JOMFP
Soon Young Park, Yuji Piao, Craig Thomas, Gregory N Fuller, John F de Groot
Cdc2-like kinase 2 (CLK2) is known as a regulator of RNA splicing that ultimately controls multiple physiological processes. However, the function of CLK2 in glioblastoma progression has not been described. Reverse-phase protein array (RPPA) was performed to identify proteins differentially expressed in CLK2 knockdown cells compared to controls. The RPPA results indicated that CLK2 knockdown influenced the expression of survival-, proliferation-, and cell cycle-related proteins in GSCs. Thus, knockdown of CLK2 expression arrested the cell cycle at the G1 and S checkpoints in multiple GSC lines...
May 3, 2016: Oncotarget
Jianbin Zhong, Xie Li, Limei Wan, Zhibang Chen, Simin Zhong, Songhua Xiao, Zhengwen Yan
NogoA is a myelin‑associated protein, which is important in the inhibition of axonal fiber growth and in regeneration following injury of the mammalian central nervous system. A previous study suggested that NogoA may be key in the process of Parkinson's disease (PD), which is the second most common chronic neurodegenerative disorder worldwide. The regulatory mechanism underlying the effect of NogoA on the process of PD remains to be fully elucidated. The present study aimed to investigate the effect and underlying mechanism of NogoA on cellular viability, apoptosis and autophagy induced by 1-methyl-4-phenylpyridinium (MPP+) in PC12 cells, a commonly used in vitro PD model...
February 2016: Molecular Medicine Reports
Santosh Kumar, Sharad Kumar Dwivedi, S S Singh, Sanjeev Kumar, R K Sundaram, Shivani, A K Mall
The objective of the present study was to examine the effect of aerobic situation on yield, physiological and biochemical traits of advanced breeding lines of rice. Experiment was conducted with two set of rice genotypes under two water regimes (aerobic and irrigated), during three consecutive wet seasons 2010-2012. Significant decrease in yield was observed in rice genotypes grown under aerobic situation as compared to the irrigated ones. Promising rice genotypes having the ability to maintain high plant biomass, harvest index, early vegetative vigour, improved physiological and biochemical traits in terms of relative water content (RWC), leaf area index (LAI), total soluble sugar, starch, protien and proline content help to sustain higher grain yield under aerobic situation...
July 2015: Journal of Environmental Biology
Ritambhra Nada, Raja Ramachandran, Ashwani Kumar, Manish Rathi, Amit Rawat, Kusum Joshi, Harbir Singh Kohli, Krishan Lal Gupta
AIMS: Immunoglobulin-G4 (IgG4)-related tubulo-interstitial nephritis (IgG4TIN) could be the first presentation of IgG4-related systemic disease. Most of the data is from the West or Japan and retrospective, with good patient outcome. METHODS: This study was carried out from April 2011 to July 2013. We report a prospective follow-up of 11 patients who presented with renal dysfunction and had histological diagnosis of IgG4TIN followed for a minimum period of 1 year or until end-stage renal disease...
July 2016: International Journal of Rheumatic Diseases
O Yu Bastrikov
Studied the level of induced oxidative modified serum proteins, depending on the stage and extent of arterial hypertension. With the progression of the severity of hypertension found a significant increase in fragmentation of oxidized protein products after their spontaneous and metal-catalyzed oxidation at a wavelength of 270 nm. The study established the association of oxidative modification of proteins and studied psychological parameters in patients with arterial hypertension. The nature of stress changes, their biochemical support is essential for the understanding of the mechanism of influence of stress on the development and course of systemic diseases, develop integration approach to the prevention of psychosomatic disorders...
April 2015: Rossiĭskii Fiziologicheskiĭ Zhurnal Imeni I.M. Sechenova
Arun Upadhyay, Ayeman Amanullah, Deepak Chhangani, Ribhav Mishra, Amit Mishra
Efficient and regular performance of Ubiquitin Proteasome System and Autophagy continuously eliminate deleterious accumulation of nonnative protiens. In cellular quality control system, E3 ubiquitin ligases are significant employees for defense mechanism against abnormal toxic proteins. Few findings indicate that lack of functions of E3 ubiquitin ligases can be a causative factor of neurodevelopmental disorders, neurodegeneration, cancer and ageing. However, the detailed molecular pathomechanism implying E3 ubiquitin ligases in cellular functions in multifactorial disease conditions are not well understood...
November 2015: Ageing Research Reviews
Balasubramanian Suriyanarayanan, Praveena Pothuraju Lakshmi, Ramachandran Sarojini Santhosh, Kandasamy Dhevendaran, Balakrishnan Priya, Shivaani Krishna
Streptomycin, an antibiotic used against microbial infections, inhibits the protein synthesis by binding to ribosomal protein S12, encoded by rpsL12 gene, and associated mutations cause streptomycin resistance. A streptomycin resistant, Lysinibacillus sphaericus DSLS5 (MIC >300 µg/mL for streptomycin), was isolated from a marine sponge (Tedania anhelans). The characterisation of rpsL12 gene showed a region having similarity to long terminal repeat sequences of murine lukemia virus which added 13 amino acids for loop formation in RpsL12; in addition, a K56R mutation which corresponds to K43R mutation present in streptomycin-resistant Escherichia coli is also present...
June 2016: Journal of Biomolecular Structure & Dynamics
S Kh Bidzhieva, K S Derbikova, I V Kublanov, E A Bonch-Osmolovskaya
Anaerobic thermophilic archaea of the genera Thermogladius and Desulfurococcus capable of a- and P3-keratin decomposition were isolated from hot springs of Kamchatka and Kunashir Island. For two of them (strains 2355k and 3008g), the presence of high-molecular mass, cell-bound endopeptidases active against nonhydrolyzed and partially hydrolyzed proteins at high values of temperature and pH was shown. Capacity for β-keratin decomposition was also found in collection strains (type strains of Desulfurococcus amylolyticus subsp...
November 2014: Mikrobiologiia
Atul A Chaudhari, Shanese L Jasper, Ejovwoke Dosunmu, Michael E Miller, Robert D Arnold, Shree R Singh, Shreekumar Pillai
BACKGROUND: Resistance of food borne pathogens such as Salmonella to existing antibiotics is of grave concern. Silver coated single walled carbon nanotubes (SWCNTs-Ag) have broad-spectrum antibacterial activity and may be a good treatment alternative. However, toxicity to human cells due to their physico-chemical properties is a serious public health concern. Although pegylation is commonly used to reduce metal nanoparticle toxicity, SWCNTs-Ag have not been pegylated as yet, and the effect of pegylation of SWCNTs-Ag on their anti-bacterial activity and cell cytotoxicity remains to be studied...
2015: Journal of Nanobiotechnology
Ganesh C Ingavle, Les W J Baillie, Yishan Zheng, Elzbieta K Lis, Irina N Savina, Carol A Howell, Sergey V Mikhalovsky, Susan R Sandeman
Polymeric cryogels are efficient carriers for the immobilization of biomolecules because of their unique macroporous structure, permeability, mechanical stability and different surface chemical functionalities. The aim of the study was to demonstrate the potential use of macroporous monolithic cryogels for biotoxin removal using anthrax toxin protective antigen (PA), the central cell-binding component of the anthrax exotoxins, and covalent immobilization of monoclonal antibodies. The affinity ligand (protein A) was chemically coupled to the reactive hydroxyl and epoxy-derivatized monolithic cryogels and the binding efficiencies of protein A, monoclonal antibodies to the cryogel column were determined...
May 2015: Biomaterials
Tian Zhou, Nanping Wang, Yang Xue, Tingting Ding, Xin Liu, Xiumei Mo, Jiao Sun
In this study, tilapia skin collagen sponge and electrospun nanofibers were developed for wound dressing. The collagen sponge was composed of at least two α-peptides, and its denaturation temperature was 44.99 °C. It did not change the number of spleen-derived lymphocytes in BALB/c mice, the ratio of CD4+/CD8+ lymphocytes, and the level of IgG or IgM in Sprague-Dawley rat. The contact angle, tensile strength, and weight loss temperature of collagen nanofibers were 21.2°, 6.72±0.44 MPa, and 300 °C, respectively...
February 11, 2015: ACS Applied Materials & Interfaces
Yan-Zhen Lu, Jia Wang, Cui-Ying Zhang, Juan Song, Bao-Hong Li, Xiao-Liang Song
OBJECTIVE: To investigate the effects of ischemic postconditioning (IPTC) on the changes of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) protein and mRNA levels in rat heart subjected to ischemia/reperfusion, and explore the mechanism by which IPTC protects myocardial interstitium following ischemic/reperfusion (I/R). METHODS: Twenty four healthy male SD rats were randomly divided into 3 groups (n = 8): sham control (SC) group, I/R group and IPTC group...
September 2014: Chinese Journal of Applied Physiology
Wei Wu, Yuzhu Xu, Xinliang He, Yan Lu, Yali Guo, Zhuoran Yin, Jungang Xie, Jianping Zhao
BACKGROUND: Although it is recognized that IL-33 plays a key role in the onset of asthma, it is currently unclear whether IL-33 acts on any other target cells besides mast cells and Th2 cells in asthma. We investigated that whether airway smooth muscle cells (ASMCs) could contribute to asthma via stimulation with IL-33. METHODS: To create a mouse model of acute asthma, murine ASMCs were isolated and cultured in vitro with IL-33. The ASMCs were divided into two groups, ASMCs from normal mice and ASMCs from ovalbumin-sensitized mice...
December 2014: Asian Pacific Journal of Allergy and Immunology
Bryan S Margulies, Sean D DeBoyace, Adrienne M Parsons, Connor G Policastro, Jessica S S Ee, Timothy S Damron
We sought to demonstrate whether there is a difference in the local mesenchymal stem cells (MSC) niche obtained from patients undergoing their first total joint replacement surgery versus those patients undergoing a revision surgery for an failing total joint implant. Bone marrow aspirates collected from patients undergoing revision total joint arthroplasty were observed to be less clonal and the expression of PDGFRα, CD51, ALCAM, endoglin, CXCL12, nestin, and nucleostemin were decreased. Revision MSC were also less able to commit to an osteoblast-lineage or an adipocyte-lineage...
May 2015: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Ratana Lim, Martha Lappas
PROBLEM: Obese pregnancies are characterised by increased inflammation. Members of the Slit/Roundabout (Robo) family are key regulators of the inflammatory response. The aim of this study was to determine the effect of (i) pre-existing maternal obesity on Slit-Robo expression in human placenta and (ii) Slit2 knockdown by siRNA in primary trophoblast cells on markers of inflammation. METHOD OF STUDY: The expression of Slit-Robo protiens was assessed in human placenta from lean (n = 15) and obese (n = 16) patients by qRT-PCR and Western blotting...
January 2015: American Journal of Reproductive Immunology: AJRI
Michael Mayo, Ahmed Abdelzaher, Edward J Perkins, Preetam Ghosh
Feed-forward loops are hierarchical three-node transcriptional subnetworks, wherein a top-level protein regulates the activity of a target gene via two paths: a direct-regulatory path, and an indirect route, whereby the top-level proteins act implicitly through an intermediate transcription factor. Using a transcriptional network of the model bacterium Escherichia coli, we confirmed that nearly all types of feed-forward loop were significantly overrepresented in the bacterial network. We then used mathematical modeling to study their dynamics by manipulating the rise times of the top-level protein concentration, termed the induction time, through alteration of the protein destruction rates...
September 2014: Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
Rotem Sigall-Boneh, Tamar Pfeffer-Gik, Idit Segal, Tsili Zangen, Mona Boaz, Arie Levine
BACKGROUND: Exclusive enteral nutrition is effective for inducing remission in active pediatric Crohn's disease. Partial enteral nutrition (PEN) with free diet is ineffective for inducing remission, suggesting that the mechanism depends on exclusion of free diet. We developed an alternative diet based on PEN with exclusion of dietary components hypothesized to affect the microbiome or intestinal permeability. METHODS: Children and young adults with active disease defined as a pediatric Crohn's disease activity index >7...
August 2014: Inflammatory Bowel Diseases
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