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protein hydrolysates

Merja T Rossi, Monika Kalde, Chaiyakorn Srisakvarakul, Nicholas J Kruger, R George Ratcliffe
Stable isotope labelling experiments are used routinely in metabolic flux analysis (MFA) to determine the metabolic phenotype of cells and tissues. A complication arises in multicellular systems because single cell measurements of transcriptomes, proteomes and metabolomes in multicellular organisms suggest that the metabolic phenotype will differ between cell types. In silico analysis of simulated metabolite isotopomer datasets shows that cellular heterogeneity confounds conventional MFA because labelling data averaged over multiple cell types does not necessarily yield averaged flux values...
November 13, 2017: Metabolites
Amanda R Noble, Norman J Maitland, Daniel M Berney, Martin G Rumsby
BACKGROUND: Phospholipases D1 and D2 (PLD1/2) hydrolyse cell membrane glycerophospholipids to generate phosphatidic acid, a signalling lipid, which regulates cell growth and cancer progression through effects on mTOR and PKB/Akt. PLD expression and/or activity is raised in breast, colorectal, gastric, kidney and thyroid carcinomas but its role in prostate cancer (PCa), the major cancer of men in the western world, is unclear. METHODS: PLD1 protein expression in cultured PNT2C2, PNT1A, P4E6, LNCaP, PC3, PC3M, VCaP, 22RV1 cell lines and patient-derived PCa cells was analysed by western blotting...
November 14, 2017: British Journal of Cancer
Michael Gray, Stuart M Linton, Benjamin J Allardyce
This study aimed to sequence and identify a glycosyl hydrolase family 9 (GHF9) endo-β-1,4-glucanase expressed in the midgut gland of the herbivorous gecarcinid land crab, Gecarcoidea natalis. Hence this would explain the gene responsible for the production of previously purified and characterised endo-β-1,4-glucanases. Three different transcripts, two complete and one partial were sequenced from cDNA and an open reading frame of 1383bp was produced. Translated, this would produce a putative protein of 460 amino acid residues, including a 16 amino acid residue signal peptide...
November 10, 2017: Gene
Kholoud Awad Al-Shamsi, Priti Mudgil, Hassan Mohamed Hassan, Sajid Maqsood
Camel milk protein hydrolysates (CMPH) were generated using proteolytic enzymes, such as alcalase, bromelain, and papain, to explore the effect on the technofunctional properties and antioxidant potential under in vitro and in real food model systems. Characterization of the CMPH via degree of hydrolysis, sodium dodecyl sulfate-PAGE, and HPLC revealed that different proteins in camel milk underwent degradation at different degrees after enzymatic hydrolysis using 3 different enzymes for 2, 4, and 6 h, with papain displaying the highest degradation...
November 8, 2017: Journal of Dairy Science
Xiaoyu Wang, Rui Ma, Xiangming Xie, Weina Liu, Tao Tu, Fei Zheng, Shuai You, Jianzhong Ge, Huifang Xie, Bin Yao, Huiying Luo
Thermophilic xylanases with high catalytic efficiency are of great interest in the biofuel, food and feed industries. This study identified a GH11 xylanase gene, Tlxyn11B, in Talaromyces leycettanus JCM12802. Recombinant TlXyn11B produced in Pichia pastoris is distinguished by high specific activity (8259 ± 32 U/mg with beechwood xylan as substrate) and excellent pH stability (from 1.0 to 10.5). The beechwood xylan hydrolysates consisted mainly of xylobiose, xylotriose and xylotetraose, thus TlXyn11B could be used for the production of prebiotic xylooligosaccharide...
November 10, 2017: Scientific Reports
Jonatan Hall-Andersen, Svend G Kaasgaard, Christian Janfelt
Most modern laundry detergents contain enzymes such as proteases, amylases, and lipases for more efficient removal of stains containing proteins, carbohydrates, and lipids during wash at low temperature. The function of the lipases is to hydrolyse the hydrophobic triglycerides from fats and oils to the more hydrophilic lipids diglycerides, monoglycerides and free fatty acids. Here, we use MALDI imaging to study the effect of enzymatic degradation of triglycerides by lipases directly on the textile surface. Textile samples were created by using swatches of different textile blends, adding a lipid stain and simulating washing cycles using well-defined detergents with lipase concentrations ranging between 0 and 0...
November 6, 2017: Chemistry and Physics of Lipids
Fang Liu, Weihua Wu, Mary B Tran-Gyamfi, James D Jaryenneh, Xun Zhuang, Ryan W Davis
BACKGROUND: First generation bioethanol production utilizes the starch fraction of maize, which accounts for approximately 60% of the ash-free dry weight of the grain. Scale-up of this technology for fuels applications has resulted in a massive supply of distillers' grains with solubles (DGS) coproduct, which is rich in cellulosic polysaccharides and protein. It was surmised that DGS would be rapidly adopted for animal feed applications, however, this has not been observed based on inconsistency of the product stream and other logistics-related risks, especially toxigenic contaminants...
November 9, 2017: Microbial Cell Factories
Alice B Nongonierma, Sara Paolella, Priti Mudgil, Sajid Maqsood, Richard J FitzGerald
Nine novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides (FLQY, FQLGASPY, ILDKEGIDY, ILELA, LLQLEAIR, LPVP, LQALHQGQIV, MPVQA and SPVVPF) were identified in camel milk proteins hydrolysed with trypsin. This was achieved using a sequential approach combining liquid chromatography tandem mass spectrometry (LC-MS/MS), qualitative/quantitative structure activity relationship (QSAR) and confirmatory studies with synthetic peptides. The most potent camel milk protein-derived DPP-IV inhibitory peptides, LPVP and MPVQA, had DPP-IV half maximal inhibitory concentrations (IC50) of 87...
April 1, 2018: Food Chemistry
Giovanni Ramírez-Torres, Noé Ontiveros, Verónica Lopez-Teros, Jesús Aurelio Ibarra-Diarte, Cuauhtémoc Reyes-Moreno, Edith Oliva Cuevas-Rodríguez, Francisco Cabrera-Chávez
Alcalase is the enzyme of choice to release antihypertensive peptides from amaranth proteins, but the hydrolysis conditions have not been optimized yet. Furthermore, in vivo assays are needed to confirm such a hypotensive effect. Our aim was to optimize the hydrolysis of amaranth protein with alcalase and to test in vivo the hypotensive effect of the hydrolysates. A response surface analysis was carried out to optimize the hydrolysis reaction. The response variable was the Angiotensin Converting Enzyme (ACE-I) inhibition...
November 9, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Kenji Sato
It has been demonstrated that the ingestion of some protein hydrolysates exerts health-promoting effects. For understanding the underlying mechanisms responsible for these effects, the identification of bioactive peptides in the target organ is crucial. For this purpose, in vitro activity-guided fractionation for peptides in the protein hydrolysate has been performed. However, the peptides in the hydrolysate may be further degraded during digestion. The concentration of the active peptides, which were identified by in vitro activity-guided fractionation, in human blood is frequently very low (nanomolar levels)...
November 8, 2017: Food & Function
Lara P Orio, Giovanna Boschin, Teresa Recca, Carlo F Morelli, Laura Ragona, Pierangelo Francescato, Anna Arnoldi, Giovanna Speranza
An hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bi-dimensional NMR experiments and LC-MS/MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR...
November 7, 2017: Journal of Agricultural and Food Chemistry
Hideyuki Suzuki, Yuko Nakafuji, Tomoki Tamura
In the present study, we demonstrate a novel use for a commercially available glutaminase that can be used as a γ-glutamyltranspeptidase in kokumi seasoning production. Soy protein and gluten were hydrolyzed using a protease isolated from Bacillus licheniformis. The resulting protein hydrolysates were γ-glutamylated with a γ-glutamyltranspeptidase, which is sold as a glutaminase from B. amyloliquefaciens, to produce kokumi seasonings. For γ-glutamylation of soy protein hydrolysate, glutamine was added to the reaction mixture...
November 7, 2017: Journal of Agricultural and Food Chemistry
Renganath Rao Ramesh, Vimudha Muralidharan, Saravanan Palanivel
Usage of the animal fleshing waste as the source of carbon and nitrogen for animal skin unhairing protease (EC 3.4.21) production along with agro-industrial wastes like wheat bran has been investigated. Thermal hydrolysis of delimed fleshing waste for 3 h yielded a fleshing hydrolysate (FH) having a protein content of 20.86 mg/mL and total solids of 46,600 ppm. The FH was lyophilized and spray dried to obtain fleshing hydrolysate powder (FHP) to be used along with wheat bran and rice bran for protease production...
November 6, 2017: Environmental Science and Pollution Research International
Meghan A Brown, Emma J Stevenson, Glyn Howatson
A number of different forms of protein and their analogues have been investigated for their efficacy in ameliorating exercise-induced muscle damage (EIMD) and recovery. Preliminary data regarding whey protein hydrolysate (WPH) supplementation are promising. However, its efficacy beyond acute eccentric/resistance exercise bouts or longer-term training programmes are limited and all investigations have been conducted in male or mixed-sex groups. This study sought to elucidate whether the benefits of WPH previously reported can be demonstrated in females following repeated-sprint exercise...
November 6, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
Niamh M Murray, Dolores O'Riordan, Jean-Christophe Jacquier, Michael O'Sullivan, Joshua L Cohen, Hildegarde Heymann, Daniela Barile, David C Dallas
Casein-hydrolysates (NaCaH) are desirable functional ingredients, but their bitterness impedes usage in foods. This study sought to validate a paper-disk approach to help evaluate bitterness in NaCaHs and to develop a food-grade approach to separate a NaCaH into distinct fractions, which could be evaluated by a sensory panel. Membrane filtration generated <0.2-μm and <3-kDa permeates. Further fractionation of the <3-kDa permeate by flash-chromatography generated four fractions using ethanol (EtOH) concentrations of 5, 10, 30 and 50%...
June 2017: Journal of Sensory Studies
Honglu Chao, Yinlong Liu, Xian Fu, Xiupeng Xu, Zhongyuan Bao, Chao Lin, Zheng Li, Yan Liu, Xiaoming Wang, Yongping You, Ning Liu, Jing Ji
iPLA2γ, calcium-independent phospholipase A2γ, discerningly hydrolyses glycerophospholipids to liberate free fatty acids. iPLA2γ-deficiency has been associated with abnormal mitochondrial function. More importantly, the iPLA2 family is causative proteins in mitochondrial neurodegenerative disorders such as parkinsonian disorders. However, the mechanisms by which iPLA2γ affects Parkinson's disease (PD) remain unknown. Mitochondrion stress has a key part in rotenone-induced dopaminergic neuronal degeneration...
November 2, 2017: Experimental Neurology
Jiaqiang Wu, Jinlong Hu, Shumiao Zhao, Mingxiong He, Guoquan Hu, Xiangyang Ge, Nan Peng
Yeasts are good candidates to utilize the hydrolysates of lignocellulose, the most abundant bioresource, for bioproducts. This study aimed to evaluate the efficiencies of single-cell protein (SCP) and xylitol production by a novel yeast strain, Candida intermedia FL023, from lignocellulosic hydrolysates and xylose. This strain efficiently assimilated hexose, pentose, and cellubiose for cell mass production with the crude protein content of 484.2 g kg(-1) dry cell mass. SCP was produced by strain FL023 using corncob hydrolysate and urea as the carbon and nitrogen sources with the dry cell mass productivity 0...
November 2, 2017: Applied Biochemistry and Biotechnology
Gilda Aiello, Carmen Lammi, Giovanna Boschin, Chiara Zanoni, Anna Arnoldi
The seed of industrial hemp is an underexploited protein source. In view of a possible use in functional foods, a hempseed protein concentrate was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these enzymes. A detailed peptidomic analysis using data-dependent acquisition showed that the numbers of peptides identified ranged from 90 belonging to 33 parent proteins in the peptic hydrolysate to 9 belonging to 6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme A reductase activity, when tested on the catalytic domain of the enzyme...
November 2, 2017: Journal of Agricultural and Food Chemistry
Tao He, Dominik Jańczewski, Shifeng Guo, Shu Mei Man, Shan Jiang, Wui Siew Tan
Polyoxazolines have received increasing attention as low-fouling materials with good stability and ease of functional group incorporation. We investigated layer-by-layer (LbL) assembly of poly(2-ethyl-2-oxazoline) (PEOX) with poly(acrylic acid) (PAA) to incorporate PEOX into thin conformal coatings with controllable thicknesses ranging from the nano- to micron range. Partial hydrolysis of PEOX (to form PEOX-I) was used to introduce secondary amine groups that enable post-assembly multilayer stabilization by heat-induced crosslinking...
October 12, 2017: Colloids and Surfaces. B, Biointerfaces
Petya Ivanova, Vesela I Chalova, Hristo Kalaydzhiev, Mariana Perifanova-Nemska, Turid Rustad, Lidia Koleva
The utilization of industrial sunflower meal to produce protein-rich products for the food industry is an alternative approach for better and more efficient use of this agricultural by-product. Sunflower meal proteins possess specific functional properties, which however need improvement to broaden their potential as supplements for delivering high--quality products for human nutrition. The aim of the study is to evaluate the combined influence of low-degree pepsin hydrolysis and transglutaminase (TG) modification on industrial sunflower meal protein isolate functionality at pH=2 to 10...
September 2017: Food Technology and Biotechnology
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