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protein hydrolysates

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https://www.readbyqxmd.com/read/28098837/casein-glycomacropeptide-hydrolysates-exert-cytoprotective-effect-against-cellular-oxidative-stress-by-up-regulating-ho-1-expression-in-hepg2-cells
#1
Tiange Li, Bin Chen, Min Du, Jiajia Song, Xue Cheng, Xu Wang, Xueying Mao
Oxidative stress is considered as an important mediator in the progression of metabolic disorders. The objective of this study was to investigate the potential hepatoprotective effects and mechanisms of bovine casein glycomacropeptide hydrolysates (GHP) on hydrogen peroxide (H₂O₂)-induced oxidative damage in HepG2 cells. Results showed that GHP significantly blocked H₂O₂-induced intracellular reactive oxygen species (ROS) generation and cell viability reduction in a dose-dependent manner. Further, GHP concentration-dependently induced heme oxygenase-1 (HO-1) expression and increased nuclear factor-erythroid 2-related factor 2 (Nrf2) nuclear translocation...
January 15, 2017: Nutrients
https://www.readbyqxmd.com/read/28088640/bioethanol-production-from-cellulosic-hydrolysates-by-engineered-industrial-saccharomyces-cerevisiae
#2
Ye-Gi Lee, Yong-Su Jin, Young-Lok Cha, Jin-Ho Seo
Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy Saccharomyces cerevisiae JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. As expected, the engineered strain (JX123) enhanced ethanol production from cellulosic hydrolysates as compared to other engineered haploid strains...
December 18, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#3
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Matheus Augusto R N da Silva, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28087620/e152a-substitution-drastically-affects-ndm-5-activity
#4
Gaurav Kumar, Bagre Issa, Debasish Kar, Sarmistha Biswal, Anindya S Ghosh
New Delhi Metallo beta-lactamase (NDM) is of significant public health concern due to its enormous potential to hydrolyse all major beta-lactams including carbapenems. Amino acid substitutions outside the active site reportedly affect NDM beta-lactamase activities. Here, the effect of amino acid substitutions in the possible omega-like loop region of NDM-5 has been elucidated. Overall, three substitution mutations near active site of NDM-5 were done, namely, E152A, S191A, and D223A and subsequently, the change in antimicrobial resistance was monitored upon expressing each mutant in a suitable host...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28079012/antioxidant-peptides-from-terrestrial-and-aquatic-plants-against-cancer
#5
Marquez-Rios Enrique, Del-Toro-Sánchez Carmen Lizette
Cancer is the leading cause of morbidity and mortality worldwide. Therefore, the search for new and less aggressive treatments is currently the focus of the anticancer research. An attractive alternative for this purpose is the use of bioactive peptides from plants. Plants live everywhere on Earth, both on land and in water, and they are a major source of diverse molecules with pharmacological potential as antioxidant peptides. Hence, this review focuses on the importance of the antioxidant activity of terrestrial and aquatic plant peptides against cancer throughout several mechanisms...
January 11, 2017: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/28078050/complete-genome-sequence-of-lutibacter-profundi-lp1-t-isolated-from-an-arctic-deep-sea-hydrothermal-vent-system
#6
Juliane Wissuwa, Sven Le Moine Bauer, Ida Helene Steen, Runar Stokke
Lutibacter profundi LP1(T) within the family Flavobacteriaceae was isolated from a biofilm growing on the surface of a black smoker chimney at the Loki's Castle vent field, located on the Arctic Mid-Ocean Ridge. The complete genome of L. profundi LP1(T) is the first genome to be published within the genus Lutibacter. L. profundi LP1(T) consists of a single 2,966,978 bp circular chromosome with a GC content of 29.8%. The genome comprises 2,537 protein-coding genes, 40 tRNA species and 2 rRNA operons. The microaerophilic, organotrophic isolate contains genes for all central carbohydrate metabolic pathways...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28076454/a-broad-ph-range-and-processive-chitinase-from-a-metagenome-library
#7
S S Thimoteo, A Glogauer, H Faoro, E M de Souza, L F Huergo, B M Moerschbacher, F O Pedrosa
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein...
January 5, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28069129/antioxidant-activity-measurement-and-potential-antioxidant-peptides-exploration-from-hydrolysates-of-novel-continuous-microwave-assisted-enzymolysis-of-the-scomberomorus-niphonius-protein
#8
Yipeng Huang, Guihua Ruan, Zhijun Qin, Haiyun Li, Yanjie Zheng
A novel continuous microwave-assisted enzymatic digestion (cMAED) method is proposed for the digestion of protein from Scomberomorus niphonius to obtain potential antioxidant peptides. In this study, bromelain was found to have a high capacity for the digestion of the Scomberomorus niphonius protein. The following cMAED conditions were investigated: protease species, microwave power, temperature, bromelain content, acidity of the substrate solution, and incubation time. At 400W, 40°C, 1500U·g(-1) bromelain, 20% substrate concentration, pH 6...
May 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28066939/the-nutrition-of-poultry-as-a-factor-affecting-litter-quality-and-foot-pad-dermatitis-an-updated-review
#9
REVIEW
S Swiatkiewicz, A Arczewska-Wlosek, D Jozefiak
Foot pad dermatitis (FPD), a condition of inflammation and necrotic lesions on the plantar surface of the footpads, is commonly observed in fast-growing broiler chickens and turkeys. FPD negatively affects the welfare of birds, performance indices and the economic profit of poultry meat production. Nutrition is an important factor affecting water intake, excreta moisture and litter quality and, in this way, the occurrence and intensity of FPD in birds. This article reviews and discusses the recent results published in the literature on the effects of nutritional factors on litter quality and FPD severity in broiler chickens and turkeys...
January 8, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28064494/influence-of-protein-phenolic-complex-on-the-antioxidant-capacity-of-flaxseed-linum-usitatissimum-l-products
#10
Fernanda Guimarães Drummond E Silva, Beatriz Miralles, Blanca Hernández-Ledesma, Lourdes Amigo, Amadeu Hoshi Iglesias, Felix Guillermo Reyes Reyes, Flavia Maria Netto
The impact of the naturally-present phenolic compounds and/or proteins on the antioxidant capacity of flaxseed products- phenolic fraction, protein concentrates and hydrolysates- before and after simulated gastrointestinal- digestion was studied. For that, whole and phenolic reduced products were assessed. Four glycosylated phenolic compounds - secoisolariciresinol and ferulic, p-coumaric and caffeic acids - were identified in flaxseed products. Phenolic fraction exerts the highest antioxidant capacity that increased by alkaline hydrolysis and by simulated gastrointestinal digestion...
January 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28058453/early-history-of-the-breeding-of-amino-acid-producing-strains
#11
Shigeru Nakamori
Amino acid production started in Japan in 1908 with the extraction of monosodium glutamate (MSG) from acid hydrolysates of proteins. In addition to extraction, other methods of amino acid production include chemical synthesis, fermentation, and enzymatic synthesis both for glutamic acid and other amino acids. In this chapter, we review the historical transition of these production methods; currently, fermentation is the chief production method of amino acids. All wild-type microorganisms possess a negative feedback control system (feedback inhibition and repression) on the enzymes within the amino acid biosynthetic pathways...
January 6, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28053783/novel-angiotensin-converting-enzyme-inhibitory-peptides-from-caseins-and-whey-proteins-of-goat-milk
#12
Hisham R Ibrahim, Ahmed S Ahmed, Takeshi Miyata
Angiotensin-converting enzyme (ACE) plays a central role in blood pressure regulation by producing the vasoconstrictor angiotensin II. The inhibition of ACE with natural inhibitors, as alternatives to avoid the side effect of synthetic drugs, is a major target in the prevention of hypertension. In this study, we examined the separated caseins and whey proteins of goat milk for the presence of ACE inhibitory peptides. Digestion of isolated whey proteins and caseins of goat milk by gastric pepsin generated soluble hydrolysates exhibiting significant inhibition of ACE compared to weak inhibition by undigested proteins...
January 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28051864/identification-and-characterization-of-glycopeptides-from-egg-protein-ovomucin-with-anti-agglutinating-activity-against-porcine-k88-enterotoxigenic-escherichia-coli-strains
#13
Xiaohong Sun, Michael G Gänzle, Jianping Wu
Ovomucin is a glycoprotein from egg white with potential to act as an anti-adhesive agent against infectious diseases. This study aimed to determine whether ovomucin or ovomucin hydrolysates could prevent adhesion to two porcine K88 enterotoxigenic Escherichia coli (ETEC) strains. Adhesion was determined in vitro using hemagglutination assay. Ovomucin hydrolysates, but not intact ovomucin, prevented ETEC adhesion to porcine erythrocytes. Ovomucin hydrolysate prepared by acid protease II exhibited the best anti-agglutinating activity against both strains; this hydrolysate was fractionated by cation exchange chromatography and reverse-phase HPLC...
January 4, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28045202/raw-mechanically-separated-chicken-meat-and-salmon-protein-hydrolysate-as-protein-sources-in-extruded-dog-food-effect-on-protein-and-amino-acid-digestibility
#14
M T Tjernsbekk, A-H Tauson, O F Kraugerud, Ø Ahlstrøm
Protein quality was evaluated for mechanically separated chicken meat (MSC) and salmon protein hydrolysate (SPH), and for extruded dog foods where MSC or SPH partially replaced poultry meal (PM). Apparent total tract digestibility (ATTD) of crude protein (CP) and amino acids (AA) in the protein ingredients and extruded foods was determined with mink (Neovison vison). The extruded dog foods included a control diet with protein from PM and grain, and two diets where MSC or SPH provided 25% of the dietary CP. Nutrient composition of the protein ingredients varied, dry matter (DM) was 944...
January 3, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28042837/purification-and-characterization-of-antioxidant-peptides-of-pseudosciaena-crocea-protein-hydrolysates
#15
Ningning Zhang, Chong Zhang, Yuanyuan Chen, Baodong Zheng
Two peptides with antioxidant activity were isolated from Pseudosciaena crocea proteins. Pseudosciaena crocea muscle was hydrolyzed with neutral protease to obtain Pseudosciaena crocea protein hydrolysates (PCPH). After ultrafiltration through molecular weight cut-off membranes of 10, 5 and 3 kDa and assessment of free radical scavenging ability, the fraction (PCPH-IV) with the highest antioxidant activity was obtained. Several purification steps, i.e., ion exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography, were applied to further purify PCPH-IV...
December 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28042769/discovery-of-an-unexplored-protein-structural-scaffold-of-serine-protease-from-big-blue-octopus-octopus-cyanea-a-new-prospective-lead-molecule
#16
Subhamay Panda, Leena Kumari
BACKGROUND: Serine proteases are a group of enzymes that hydrolyses the peptide bonds in proteins. In mammals, these enzymes help in the regulation of several major physiological functions such as digestion, blood clotting, responses of immune system, reproductive functions and the complement system. OBJECTIVE: Serine proteases obtained from the venom of Octopodidae family is a relatively unexplored area of research. In the present work, we tried to effectively utilize comparative composite molecular modeling technique...
January 2, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/28040661/foam-fractionation-as-a-tool-to-study-the-air-water-interface-structure-function-relationship-of-wheat-gluten-hydrolysates
#17
Arno G B Wouters, Ine Rombouts, Nele Schoebrechts, Ellen Fierens, Kristof Brijs, Christophe Blecker, Jan A Delcour
Enzymatic hydrolysis of wheat gluten protein improves its solubility and produces hydrolysates with foaming properties which may find applications in food products. First, we here investigated whether foam-liquid fractionation can concentrate wheat gluten peptides with foaming properties. Foam and liquid fractions had high and very low foam stability (FS), respectively. In addition, foam fractions were able to decrease surface tension more pronouncedly than un-fractionated samples and liquid fractions, suggesting they are able to arrange themselves more efficiently at an interface...
December 22, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28036002/novel-peptide-with-specific-calcium-binding-capacity-from-schizochytrium-sp-protein-hydrolysates-and-calcium-bioavailability-in-caco-2-cells
#18
Xixi Cai, Jiaping Lin, Shaoyun Wang
Peptide-calcium can probably be a suitable supplement to improve calcium absorption in the human body. In this study, a specific peptide Phe-Tyr (FY) with calcium-binding capacity was purified from Schizochytrium sp. protein hydrolysates through gel filtration chromatography and reversed phase HPLC. The calcium-binding capacity of FY reached 128.77 ± 2.57 μg/mg. Results of ultraviolet spectroscopy, fluorescence spectroscopy, and infrared spectroscopy showed that carboxyl groups, amino groups, and amido groups were the major chelating sites...
December 27, 2016: Marine Drugs
https://www.readbyqxmd.com/read/28027395/endo-lysosomal-and-autophagic-dysfunction-a-driving-factor-in-alzheimer-s-disease
#19
REVIEW
L S Whyte, A A Lau, K M Hemsley, J J Hopwood, T J Sargeant
Alzheimer's disease (AD) is the most common cause of dementia and its prevalence will increase significantly in the coming decades. Although important progress has been made, fundamental pathogenic mechanisms as well as most hereditary contributions to the sporadic form of the disease remain unknown. In this review, we examine the now substantial links between AD pathogenesis and lysosomal biology. The lysosome hydrolyses and processes cargo delivered by multiple pathways, including endocytosis and autophagy...
December 27, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28018000/development-of-protein-fortified-mango-based-ready-to-serve-beverage
#20
Deep N Yadav, R K Vishwakarma, Sanket Borad, Sangita Bansal, Arvind K Jaiswal, Monika Sharma
Fruit drinks contain negligible amount of protein as nutritional component. Fortification of fruit drinks with protein is a challenge due to protein stability in acidic and ionic environment. Mango ready-to-serve (RTS) beverage was fortified with modified whey protein and its rheological properties were studied. Whey protein was hydrolysed with papain to improve its stability in acidic medium. The water holding capacity of whey protein increased about two times after hydrolysis. Hydrolysed and native whey protein was used at 2, 3 and 4% levels for fortification of mango based RTS beverage...
October 2016: Journal of Food Science and Technology
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