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protein hydrolysates

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https://www.readbyqxmd.com/read/28433916/a-novel-method-for-contributing-to-composting-start-up-at-low-temperature-by-inoculating-cold-adapted-microbial-consortium
#1
Xin-Yu Xie, Yue Zhao, Qing-Hong Sun, Xue-Qin Wang, Hong-Yang Cui, Xu Zhang, Yan-Jie Li, Zi-Min Wei
Low temperature climate presented a technical challenge to start-up composting in northern region of China. This study investigated if the cold-adapted microbial consortium (CAMC) could promote composting start-up at low temperature. In this work, the CAMC was inoculated when food waste was composted at 10°C. The results showed that inoculating CAMC accelerated the piles temperature effectively, the piles passed through the start-up period within 37h. Moreover, the inoculants could enhance the abundances of dominant strains related to organic matters degradation rate...
April 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433905/pretreating-wheat-straw-by-phosphoric-acid-plus-hydrogen-peroxide-for-enzymatic-saccharification-and-ethanol-production-at-high-solid-loading
#2
Jingwen Qiu, Lunjie Ma, Fei Shen, Gang Yang, Yanzong Zhang, Shihuai Deng, Jing Zhang, Yongmei Zeng, Yaodong Hu
Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP) for enzymatic hydrolysis and ethanol fermentation at high solid loadings. Results indicated solid loading could reach 20% with 77.4% cellulose-glucose conversion and glucose concentration of 164.9g/L in hydrolysate, it even was promoted to 25% with only 3.4% decrease on cellulose-glucose conversion as the pretreated-wheat straw was dewatered by air-drying. 72.9% cellulose-glucose conversion still was achieved as the minimized enzyme input of 20mg protein/g cellulose was employed for hydrolysis at 20% solid loading...
April 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28432100/casein-kinase-i-isoform-hrr25-is-a-negative-regulator-of-haa1-in-the-weak-acid-stress-response-pathway-in-saccharomyces-cerevisiae
#3
Morgan E Collins, Joshua J Black, Zhengchang Liu
Haa1 is a transcription factor that adapts Saccharomyces cerevisiae cells to weak organic acid stresses by activating the expression of various genes. Many of these genes encode membrane proteins such as TPO2 and YRO2 How Haa1 is activated by weak acids is not clear. Here, we show that casein kinase I isoform Hrr25 is an important negative regulator of Haa1. Haa1 is known to be multiply phosphorylated. We found that mutations in HRR25 lead to reduced Haa1 phosphorylation and increased expression of Haa1-target genes, and that Hrr25 interacts with Haa1...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28428821/genome-wide-search-for-candidate-genes-for-yeast-robustness-improvement-against-formic-acid-reveals-novel-susceptibility-trk1-and-positive-regulators-and-resistance-haa1-regulon-determinants
#4
Sílvia F Henriques, Nuno P Mira, Isabel Sá-Correia
BACKGROUND: Formic acid is an inhibitory compound present in lignocellulosic hydrolysates. Understanding the complex molecular mechanisms underlying Saccharomyces cerevisiae tolerance to this weak acid at the system level is instrumental to guide synthetic pathway engineering for robustness improvement of industrial strains envisaging their use in lignocellulosic biorefineries. RESULTS: This study was performed to identify, at a genome-wide scale, genes whose expression confers protection or susceptibility to formic acid, based on the screening of a haploid deletion mutant collection to search for these phenotypes in the presence of 60, 70 and 80 mM of this acid, at pH 4...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28427643/improving-the-enzymolysis-efficiency-of-potato-protein-by-simultaneous-dual-frequency-energy-gathered-ultrasound-pretreatment-thermodynamics-and-kinetics
#5
Yu Cheng, Yun Liu, Juan Wu, Prince Ofori Donkor, Ting Li, Haile Ma
The thermodynamics and kinetics of traditional and simultaneous dual frequency energy-gathered ultrasound (SDFU) assisted enzymolysis of potato protein were investigated to get the knowledge of the mechanisms on the SDFU's promoting efficiency during enzymolysis. The concentration of potato protein hydrolysate and parameters of thermodynamic and kinetic during traditional and SDFU assisted enzymolysis were determined. The results showed that potato protein hydrolysate concentration of SDFU assisted enzymolysis was higher than traditional enzymolysis at the hydrolysis time of 60min (p<0...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28425100/mass-spectrometry-analysis-and-in-silico-prediction-of-allergenicity-of-peptides-in-tryptic-hydrolysates-of-the-proteins-from-ruditapes-philippinarum
#6
Yue Yu, Hongwei Liu, Maolin Tu, Meiling Qiao, Zhenyu Wang, Ming Du
BACKGROUND: The Ruditapes philippinarum is nutrient-rich and widely-distributed, but little attentions had been paid on the identification and characterization of bioactive peptides in the bivalve. In this study, we evaluated the peptides of the Ruditapes philippinarum enzymolysised by trypsin using a combination of UPLC separation and a ESI-Q-TOF-MS/MS spectrometer, followed by data processing, sequence-similarity database searching. The potential allergenicity of the peptides was assessed in silico...
April 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28424716/growth-stimulatory-effects-and-genome-wide-transcriptional-changes-produced-by-protein-hydrolysates-in-maize-seedlings
#7
Chiara Santi, Anita Zamboni, Zeno Varanini, Tiziana Pandolfini
Protein hydrolysates are an emerging class of crop management products utilized for improving nutrient assimilation and mitigating crop stress. They generally consist of a mixture of peptides and free amino acids derived from the hydrolysis of plant or animal sources. The present work was aimed at studying the effects and the action mechanisms of a protein hydrolysate derived from animal residues on maize root growth and physiology in comparison with the effects induced by either free amino acids or inorganic N supply...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28418498/suppression-by-an-rar-%C3%AE-agonist-of-collagen-degradation-mediated-by-corneal-fibroblasts
#8
Kazuhiro Kimura, Hongyan Zhou, Tomoko Orita, Masaaki Kobayashi, Teruo Nishida, Koh-Hei Sonoda
Purpose: To examine the role of retinoic acid receptor (RAR) isoforms in interleukin-1β (IL-1β)-induced collagen degradation by corneal fibroblasts. Methods: Primary rabbit corneal fibroblasts embedded in a three-dimensional collagen gel were incubated with or without all-trans retinoic acid (ATRA), the RAR-α agonist Am580, the RAR-β agonist AC55649, or the RAR-γ agonist R667. Collagen degradation was determined by measurement of hydroxyproline produced in acid hydrolysates of culture supernatants...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28416855/production-and-characterization-of-functional-properties-of-protein-hydrolysates-from-egg-shell-membranes-by-lactic-acid-bacteria-fermentation
#9
Surangna Jain, Anil Kumar Anal
This study aimed to ferment the chicken eggshell membrane (ESM) using the lactic acid bacteria, Lactobacillus plantarum for preparation of functional and bioactive protein hydrolysates. Cultivation at an initial pH of 8.0 for 36 h resulted in maximum protein concentration (177.3 mg/g) and degree of hydrolysis (25.1%) of the hydrolysates. Fermentation resulted in the production of hydrolysates that demonstrated excellent solubility (90.7%), good foaming capacity (36.7%) and emulsification activity (94.6 m(2)/g)...
April 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28413445/valorization-of-untreated-rice-bran-towards-bioflocculant-using-a-lignocellulose-degrading-strain-and-its-use-in-microalgal-biomass-harvest
#10
Cong Liu, Yan Hao, Jihong Jiang, Weijie Liu
BACKGROUND: Microalgae are currently considered as a promising feedstock for the production of biofuels and high-value products. However, the efficient harvest of microalgal biomasses from their culture broth is a major challenge. The harvesting of algal biomass by flocculation combined with gravity sedimentation is more convenient and cost-effective than traditional methods such as centrifugation and filtration. Compared to inorganic and chemically synthetic flocculants, bioflocculants are a suitable choice for microalgal harvest due to their biodegradable and nontoxic properties...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28407953/antioxidant-potential-of-edible-mushroom-agaricus-bisporus-protein-hydrolysates-and-their-ultrafiltration-fractions
#11
Benard Muinde Kimatu, Liyan Zhao, Yuan Biao, Gaoxing Ma, Wenjian Yang, Fei Pei, Qiuhui Hu
Mushroom protein isolate (MPI) from Agaricus bisporus was hydrolyzed using Alcalase, Pancreatin, Flavourzyme, Alcalase-Pancreatin and Alcalase-Flavourzyme. The obtained hydrolysates (MPHs) were ultrafiltered to generate peptide fractions (UFs) of molecular sizes (<1, 1-3, 3-5 and 5-10kDa). The electrophoretic profile results indicated that the enzymatic systems were efficient in hydrolyzing the MPI into low molecular weight peptides. Hydrolysate yields of >57% and protein recoveries of >43% were obtained...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407942/effect-of-ultrasound-pretreatment-and-maillard-reaction-on-structure-and-antioxidant-properties-of-ultrafiltrated-smooth-hound-viscera-proteins-sucrose-conjugates
#12
Ola Abdelhedi, Leticia Mora, Ines Jemil, Mourad Jridi, Fidel Toldrá, Moncef Nasri, Rim Nasri
The effect of ultrasound (US) pre-treatment on the evolution of Maillard reaction (MR), induced between low molecular weight (LMW) peptides and sucrose, was studied. LMW peptides (<1kDa) were obtained by the ultrafiltration of smooth hound viscera protein hydrolysates, produced by Neutrase, Esperase and Purafect. MR was induced by heating the LMW peptides in the presence of sucrose for 2h at 90°C, without or with US pre-treatment. During the reaction, a marked decrease in pH values, coupled to the increase in colour of the Maillard reaction products (MRPs), were recorded...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407892/molecular-characterization-and-bio-functional-property-determination-using-sds-page-and-rp-hplc-of-protein-fractions-from-two-nigella-species
#13
Muhammad H Alu'datt, Taha Rababah, Mohammad N Alhamad, Moh'd Alodat, Majdi A Al-Mahasneh, Sana Gammoh, Khalil Ereifej, Ali Almajwal, Stan Kubow
This study aimed to investigate the molecular and bio-functional properties of protein fractions from Nigella damascena and Nigella arvensis, including the albumin, globulin, glutein-1, glutein-2 and prolamin fractions. Protein subunits were not observed in globulin and prolamin fractions. No peaks appeared in RP-HPLC chromatograms of globulin for either species. Two predominant peaks were observed in the RP-HPLC profiles of all protein fractions. Proteins separated by RP-HPLC have potential inhibitory and antioxidant activities in all fractions...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28405910/omics-analysis-of-acetic-acid-tolerance-in-saccharomyces-cerevisiae
#14
REVIEW
Peng Geng, Liang Zhang, Gui Yang Shi
Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the yeast responses to acetic acid stress is essential for improving acetic acid tolerance and ethanol production. Although 329 genes associated with acetic acid tolerance have been identified in the Saccharomyces genome and included in the database ( http://www...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28395818/effects-of-different-proteases-on-iron-absorption-property-of-egg-white-hydrolysates
#15
Yasumi Horimoto, Loong-Tak Lim
One of the causes of iron deficiency in human is poor absorption of non-heme iron from the diet. While proteins from meats have been reported in the literature to enhance the absorption of non-heme iron, other proteins, such as those from egg, are known to inhibit iron absorption. The objective of this study is to investigate non-heme iron binding property of egg white proteins hydrolyzed using pepsin and a combination of bacterial/fungal proteases. The iron bioavailability of non-heme iron, in the presence of egg white (EW) hydrolysates, was evaluated in vitro using a tissues culture model system - rat intestinal epithelial cells (IEC-6)...
May 2017: Food Research International
https://www.readbyqxmd.com/read/28394291/in-vitro-antioxidant-activities-of-enzymatic-hydrolysate-from-schizochytrium-sp-and-its-hepatoprotective-effects-on-acute-alcohol-induced-liver-injury-in-vivo
#16
Xixi Cai, Ana Yan, Nanyan Fu, Shaoyun Wang
Schizochytrium protein hydrolysate (SPH) was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight (MW) of SPH was principally concentrated at 180-3000 Da (52.29%). SPH was divided into two fractions by ultrafiltration: SPH-I (MW < 3 kDa) and SPH-II (MW > 3 kDa). Besides showing lipid peroxidation inhibitory activity in vitro, SPH-I exhibited high DPPH and ABTS radicals scavenging activities with IC50 of 350 μg/mL and 17...
April 10, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28393188/a-heptameric-peptide-purified-from-spirulina%C3%A2-sp-gastrointestinal-hydrolysate-inhibits-angiotensin-i-converting-enzyme-and-angiotensin%C3%A2-ii-induced-vascular-dysfunction-in-human-endothelial-cells
#17
Seong-Yeong Heo, Seok-Chun Ko, Chang Su Kim, Gun-Woo Oh, Bomi Ryu, Zhong-Ji Qian, Geunhyung Kim, Won Sun Park, Il-Whan Choi, Thi Tuong Vy Phan, Soo-Jin Heo, Do-Hyung Kang, Myunggi Yi, Won-Kyo Jung
In this study, a marine microalga Spirulina sp.-derived protein was hydrolyzed using gastrointestinal enzymes to produce an angiotensin I (Ang I)-converting enzyme (ACE) inhibitory peptide. Following consecutive purification, the potent ACE inhibitory peptide was composed of 7 amino acids, Thr-Met‑Glu‑Pro‑Gly‑Lys-Pro (molecular weight, 759 Da). Analysis using the Lineweaver-Burk plot and molecular modeling suggested that the purified peptide acted as a mixed non-competitive inhibitor of ACE...
May 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28392464/anticancer-and-cancer-preventive-compounds-from-edible-marine-organisms
#18
REVIEW
Marta Correia-da-Silva, Emília Sousa, Madalena M M Pinto, Anake Kijjoa
A direct impact of food on health, which demonstrates that dietary habit is one of the most important determinants of chronic diseases such as cancers, has led to an increased interest of the consumers toward natural bioactive compounds as functional ingredients or nutraceuticals. Epidemiological studies revealed that the populations of many Asian countries with high consumption of fish and seafood have low prevalence of particular type of cancers such as lung, breast, colorectal and prostate cancers. This observation has led to extensive investigations of the benefits of compounds present in edible marine organisms such as fish, marine invertebrates (mollusks, echinoderms) and marine algae as cancer chemopreventive agents...
April 6, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/28391867/ultrasensitive-immunoassay-for-monocrotaline-using-monoclonal-antibody-produced-by-n-n-carbonyldiimidazole-mediated-hapten-carrier-protein-conjugates
#19
Seiichi Sakamoto, Rika Nagamitsu, Gorawit Yusakul, Tomofumi Miyamoto, Hiroyuki Tanaka, Satoshi Morimoto
Monocrotaline (MCT) is a naturally occurring pyrrolizidine alkaloid mainly produced in plant genus Crotalaria (Fabaceae), in which various toxicities have been reported. Recently, demand of quantitative analysis for determination/detection of MCT has increased because MCT in plants can be transferred to human by food chain, leading to intoxication. In this study, monoclonal antibody (MAb) against MCT (MAb 4B8) was produced by unique one-pot conjugation between hydrolysate of MCT, retronecine (RN) and consecutive lysine residues of bovine-serum albumin (BSA) through N,N'-carbonyldiimidazole (CDI) mediated method...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28389041/pretreated-algal-bloom-as-a-substantial-nutrient-source-for-microalgae-cultivation-for-biodiesel-production
#20
Priyanka Jain, Neha Arora, Juhi Mehtani, Vikas Pruthi, C B Majumder
In the present investigation, toxic algal bloom, a copious and low-cost nutrient source was deployed for cultivating Chlorella pyrenoidosa. Various pre-treatment methods using combinations of acid/alkali and autoclave/microwave were tested for preparing hydrolysates and compared with minimal media (BG-11). Acid autoclave treatment resulted in maximum carbon, nitrogen and phosphorous content which substantially boosted the growth of the microalgal cells (4.36g/L) as compared to rest of the media. The microalga grown in this media also showed enhanced lipid content (43...
March 28, 2017: Bioresource Technology
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