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protein hydrolysates

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https://www.readbyqxmd.com/read/28805378/in-vitro-modulation-of-renin-angiotensin-system-enzymes-by-amaranth-amaranthus-hypochondriacus-protein-derived-peptides-alternative-mechanisms-different-from-ace-inhibition
#1
Alejandra V Quiroga, Paula Aphalo, Agustina Estefanía Nardo, María Cristina Añón
Among the factors affecting the development of cardiovascular diseases, hypertension is one of the most important. Research done on amaranth proteins has demonstrated their hypotensive capacity in vivo and in vitro; nevertheless the mechanism underlying this effect remains unclear. The aim of this study was to analyze in vitro the inhibition of peptides derived from an amaranth hydrolysate (AHH) on other RAS enzymes other than ACE. The chymase and renin activities were studied. AHH was not able to inhibit chymase activity although a dose-response effect was found on renin activity (IC50 0...
August 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28802008/mechanism-study-of-multimode-ultrasound-pretreatment-on-the-enzymolysis-of-wheat-gluten
#2
Yanyan Zhang, Jing Li, Suyun Li, Haile Ma, Hua Zhang
BACKGROUND: Ultrasound pretreatment could improve the angiotensin-I converting enzyme (ACE) inhibitory activity of hydrolysates of wheat gluten (WG). The working mode of ultrasound has an important effect on the enzymatic hydrolysis of protein. RESULTS: The results showed that the optimum working mode of ultrasound was alternate dual-frequency mode (20/35 kHz), substrate concentration was 30 g L(-1) , initial temperature of the suspension was 30 °C, ultrasound pretreatment time was 10 min and power density was 150 W L(-1) ...
August 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28798909/analysis-of-urine-composition-in-type-ii-diabetic-mice-after-intervention-therapy-using-holothurian-polypeptides
#3
Yanyan Li, Jiajie Xu, Xiurong Su
Hydrolysates and peptide fractions (PF) obtained from sea cucumber with commercial enzyme were studied on the hyperglycemic and renal protective effects on db/db rats using urine metabolomics. Compared with the control group the polypeptides from the two species could significantly reduce the urine glucose and urea. We also tried to address the compositions of highly expressed urinary proteins using a proteomics approach. They were serum albumins, AMBP proteins, negative trypsin, elastase, and urinary protein, GAPDH, a receptor of urokinase-type plasminogen activator (uPAR), and Ig kappa chain C region...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28791512/purification-of-angiotensin-i-converting-enzyme-ace-inhibitory-peptides-from-casein-hydrolysate-by-imac-ni-2
#4
Shanguang Wu, Xuezhen Feng, Yuan Lu, Yuting Lu, Saisai Liu, Yuhong Tian
Casein proteins were hydrolyzed by papain to identify inhibitory peptides of angiotensin I-converting enzyme (ACE). The hydrolysate was fractionized by immobilized metal affinity chromatography (IMAC-Ni(2+)). The fraction with high ACE inhibitory activity was enriched and further chromatographed on a reverse-phase column to yield four fractions. Among the fractions, the L4 fraction exhibited the highest ACE inhibitory activity and was identified by sequence analysis as Trp-Tyr-Leu-His-Tyr-Ala (WYLHYA), with IC50 value of 16...
August 8, 2017: Amino Acids
https://www.readbyqxmd.com/read/28782925/adsorption-and-separation-of-aromatic-amino-acids-from-aqueous-solutions-using-metal-organic-frameworks
#5
Dries Jonckheere, Julian A Steele, Birgit Claes, Bart Bueken, Laurens Claes, Bert Lagrain, Maarten B J Roeffaers, Dirk E De Vos
Metal-organic frameworks (MOFs) are investigated for the adsorption of aromatic amino acids L-phenylalanine (L-Phe), L-tryptophan (L-Trp) and L-tyrosine (L-Tyr) from aqueous solutions. After screening a range of water-stable MOFs, the hydrophobic Zr-MOF MIL-140C emerged as the most performant material, exhibiting uptakes of 15 wt% for L-Trp and 20 wt% for L-Phe. These uptakes are 5 to 10 wt% higher than those of large-pore zeolites Beta and Y. Both single-compound and competitive adsorption isotherms for L-Phe and L-Trp were experimentally obtained at the natural pH of these amino acid mixtures without additional pH-modification (pH 6...
August 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28780774/changes-in-haematopoietic-progenitor-colony-differentiation-and-proliferation-and-the-production-of-different-abzymes-in-eae-mice-treated-with-dna
#6
Kseniya S Aulova, Ludmila B Toporkova, Julia A Lopatnikova, Alina A Alshevskaya, Sergei V Sennikov, Valentina N Buneva, Thomas Budde, Sven G Meuth, Nelly A Popova, Irina A Orlovskaya, Georgy A Nevinsky
Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice...
August 5, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28777927/carbohydrate-and-protein-intake-during-exertional-heat-stress-ameliorates-intestinal-epithelial-injury-and-small-intestine-permeability
#7
Rhiannon M J Snipe, Anthony Khoo, Cecilia M Kitic, Peter Gibson, Ricardo J S Costa
BACKGROUND: Exertional-heat stress (EHS) disturbs the integrity of the gastrointestinal tract leading to endotoxaemia and cytokinaemia, which have symptomatic and health implications. This study aimed to determine the effects of carbohydrate and protein intake during EHS on gastrointestinal integrity, symptoms and systemic responses. METHODS: Eleven (male n=6, female n=5) endurance runners completed 2h running at 60% V̇O2max in 35°C ambient temperature on three occasions in randomised order, consuming water (WATER) or 15g glucose (GLUC) or energy-matched whey protein hydrolysate (WPH) before and every 20min during EHS...
August 4, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28771748/extraction-of-unsaturated-fatty-acid-rich-oil-from-common-carp-cyprinus-carpio-roe-and-production-of-defatted-roe-hydrolysates-with-functional-antioxidant-and-antibacterial-properties
#8
Sakhi Ghelichi, Bahareh Shabanpour, Parastoo Pourashouri, Mona Hajfathalian, Charlotte Jacobsen
BACKGROUND: Common carp roe is a rich protein and oil source, which is usually discarded with no specific use. The aims of this study were to extract oil from the discarded roe and examine functional, antioxidant, and antibacterial properties of defatted roe hydrolysates (CDRHs) at various degrees of hydrolysis (DH). RESULTS: Gas chromatography (GC) of fatty acid methyl esters (FAMEs) revealed that common carp roe oil contained high level of unsaturated fatty acids...
August 3, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28770578/detection-of-ige-reactive-proteins-in-hydrolysed-dog-foods
#9
Olivier Roitel, Lionel Bonnard, Alexandre Stella, Odile Schiltz, Delphine Maurice, Gaël Douchin, Sandrine Jacquenet, Claude Favrot, Bernard E Bihain, Nicolas Couturier
BACKGROUND: Commercial hydrolysed diets are used for the diagnosis of food allergy in dogs. The cleaved parent proteins are presumed to be too small to elicit an allergic response by reacting with allergen-specific immunoglobin E (IgE). OBJECTIVES: To evaluate three commercial hydrolysed dog diets for proteins. ANIMALS: Sera were collected from dogs with suspected food allergy. METHODS: Two batches of each hydrolysed diet were examined by electrophoresis and visualized by Coomassie blue, silver nitrate staining and IgE immunoblotting...
August 2, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28764059/a-two-step-enzymatic-modification-method-to-reduce-immuno-reactivity-of-milk-proteins
#10
Srinivasan Damodaran, Yan Li
A two-step enzymatic approach to reduce immuno-reactivity of whey protein isolate and casein has been studied. The method involves partial hydrolysis of proteins with proteases, followed by repolymerization with microbial transglutaminase. Whey protein isolate partially hydrolyzed with chymotrypsin, trypsin, or thermolysin retained about 80%, 30%, and 20% of the original immuno-reactivity, respectively. Upon repolymerization the immuno-reactivity decreased to 45%, 35%, and 5%, respectively. The immuno-reactivity of hydrolyzed and repolymerized casein was negligible compared to native casein...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28764006/potential-antioxidant-peptides-produced-from-whey-hydrolysis-with-an-immobilized-aspartic-protease-from-salpichroa-origanifolia-fruits
#11
Gabriela Fernanda Rocha, Francisco Kise, Adriana Mabel Rosso, Mónica Graciela Parisi
An aspartic protease from Salpichroa origanifolia fruits was successfully immobilized onto an activated support of glutaraldehyde agarose. The immobilized enzyme presented higher thermal stability than the free enzyme from 40°C to 50°C and high reusability, retaining 54% of the initial activity after ten cycles of the process. Whey protein concentrates (WPC) were hydrolyzed with both free and immobilized enzyme, reaching a similar degree of hydrolysis of approximately 6-8% after 20h. In addition, the immobilized derivate hydrolyzed α-lactalbumin protein with a higher affinity than β-lactoglobulin...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763949/physical-and-oxidative-stability-of-fish-oil-in-water-emulsions-fortified-with-enzymatic-hydrolysates-from-common-carp-cyprinus-carpio-roe
#12
Sakhi Ghelichi, Ann-Dorit Moltke Sørensen, Pedro J García-Moreno, Mona Hajfathalian, Charlotte Jacobsen
Physical and oxidative stability of 5% (by weight) cod liver oil-in-water emulsions fortified with common carp (C. carpio) roe protein hydrolysate (CRPH) were examined. CRPH was obtained by enzymatic hydrolysis of discarded roe by using Alcalase 2.4L for 30, 60, 90, and 120min to yield different degrees of hydrolysis (DH). All the hydrolysates showed in vitro antioxidant activity in terms of radical scavenging and chelating properties. CRPH-containing emulsions had significantly smaller droplets than control (p<0...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763132/hydrolysed-ovalbumin-offers-more-effective-preventive-and-therapeutic-protection-against-egg-allergy-than-the-intact-protein
#13
D Lozano-Ojalvo, L Pérez-Rodríguez, A Pablos-Tanarro, E Molina, R López-Fandiño
BACKGROUND: The application of specific immunotherapy to stimulate oral tolerance towards food allergens is hampered by the high frequency of adverse side effects and the excessive duration of the treatments. OBJECTIVE: In this work, a hydrolysate of ovalbumin with pepsin (OP), selected for its low IgE reactivity and Th2-stimulating capacity, was assayed for its ability to prevent and treat allergy to egg white (EW). METHODS: As a first step, the safety of OP, in terms of the absence of sensitizing and eliciting potential, was evaluated in BALB/c mice...
August 1, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28761106/overall-process-of-using-a-valerate-dominant-sludge-hydrolysate-to-produce-high-quality-polyhydroxyalkanoates-pha-in-a-mixed-culture
#14
Jiuxiao Hao, Xiujin Wang, Hui Wang
The overall process of polyhydroxyalkanoates (PHA) production in a mixed culture fed by thermophilic fermented valerate-dominant sludge hydrolysate with high-level soluble organics (proteins and carbohydrates) and nutrients (nitrogen and phosphorus) was investigated in this study. The valerate-dominant hydrolysate was fed to enrich a PHA culture with an increasing concentration, and the enriched culture displayed a strong PHA-producing capacity under feast-famine conditions. Valerate in the feedstock was preferentially utilized over acetate and butyrate, and its uptake correlated with the production of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV)...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759785/in%C3%A2-vitro-cleavage-of-bioactive-peptides-by-peptidases-from-bothrops-jararaca-venom-and-its-neutralization-by-bothropic-antivenom-produced-by-butantan-institute-major-contribution-of-serine-peptidases
#15
Alexandre Kazuo Kuniyoshi, Roberto Tadashi Kodama, Luis Henrique Ferreira Moraes, Bruno Duzzi, Leo Kei Iwai, Ismael Feitosa Lima, Daniela Cajado-Carvalho, Fernanda Vieira Portaro
In Brazil, envenomation by Bothrops pitvipers is responsible for over 73% of snakebites, and their venom is a rich source of proteolytic enzymes. Most studies have demonstrated that Bothrops jararaca venom acts on macromolecular substrates, causing an imbalance in the victim's hemostatic system. In contrast, fewer studies have examined the proteolytic activity on small molecules such as peptides. In this study, we used a set of bioactive peptides (insulin B chain, Met-enkephalin, Leu-enkephalin, neuropeptide Y, peptide YY, pancreatic polypeptide, substance P and somatostatin) to identify new peptide substrates for the metallopeptidases and serine peptidases from the B...
July 28, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28755312/mouse-nudt13-is-a-mitochondrial-nudix-hydrolase-with-nad-p-h-pyrophosphohydrolase-activity
#16
Salama R Abdelraheim, David G Spiller, Alexander G McLennan
The mammalian NUDT13 protein possesses a sequence motif characteristic of the NADH pyrophosphohydrolase subfamily of Nudix hydrolases. Due to the persistent insolubility of the recombinant product expressed in Escherichia coli, active mouse Nudt13 was expressed in insect cells from a baculovirus vector as a histidine-tagged recombinant protein. In vitro, it efficiently hydrolysed NADH to NMNH and AMP and NADPH to NMNH and 2',5'-ADP and had a marked preference for the reduced pyridine nucleotides. Much lower activity was obtained with other nucleotide substrates tested...
July 28, 2017: Protein Journal
https://www.readbyqxmd.com/read/28754686/llome-does-not-release-cysteine-cathepsins-to-the-cytosol-but-inactivates-them-in-transiently-permeabilized-lysosomes
#17
Urska Repnik, Marita Borg Distefano, Martin Tobias Speth, Matthew Yoke Wui Ng, Cinzia Progida, Bernard Hoflack, Jean Gruenberg, Gareth Griffiths
L-leucyl-L-leucine methyl ester (LLOMe) induces apoptosis, which is thought to be mediated by release of lysosomal cysteine cathepsins from permeabilized lysosomes into the cytosol. Here, we demonstrated in HeLa cells that at apoptotic as well as sub-apoptotic concentrations LLOMe caused rapid and complete lysosomal membrane permeabilization (LMP), evidenced by loss of the proton gradient and release into the cytosol of internalized lysosomal markers below 10K molecular weight. However, there was no evidence for the release of cysteine cathepsins B and L into the cytosol; rather they remained within lysosomes, where they were rapidly inactivated and degraded...
July 28, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28738702/combined-effects-of-whey-protein-hydrolysates-and-probiotics-on-oxidative-stress-induced-by-an-iron-overloaded-diet-in-rats
#18
Eunju Park, Hyun-Dong Paik, Seung-Min Lee
Whey proteins possess antioxidant properties, and probiotics have various health-promoting effects. We investigated the effects of whey protein hydrolysates (WPHs) and probiotics in rats exposed to oxidative stress induced by iron-overload diet (IOL). Rats were divided into control (CTRL), IOL (0.2% ferrous sulphate), WPH (10%), probiotic (PB) mixture (Lactococcus lactis NK34 and B. polyfermenticus SCD), and WPH + PB group for 6 weeks. Average leukocytes and colonocytes tail moments were increased in IOL compared to CTRL but decreased in other groups...
July 25, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28735471/effect-of-processing-conditions-on-degree-of-hydrolysis-ace-inhibition-and-antioxidant-activities-of-protein-hydrolysate-from-acetes-indicus
#19
Vignaesh Dhanabalan, Martin Xavier, Nagalakshmi Kannuchamy, Kurukkan Kunnath Asha, Chongtham Baru Singh, Amjad Balange
Protein hydrolysate was prepared from Acetes indicus which is a major bycatch among non-penaeid prawn landings of India. Hydrolysis conditions (enzyme to substrate ratio and time) for preparing protein hydrolysates using alcalase enzyme were optimized by response surface methodology using central composite design. The optimum conditions for enzyme-substrate ratio (mL/100 g) of 1.57, 1.69, 1.60, 1.56, and 1.50 and for hydrolysis time of 97.18, 96.5, 98.15 min, 102.48, and 88.44 min were established for attaining maximum yield, degree of hydrolysis, 2,2-diphenyl-1-picrylhydrazyl, angiotensin I-converting enzyme-inhibiting activity, and metal-chelating activity, respectively...
July 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28731389/protease-hydrolysates-of-filefish-thamnaconus-modestus-byproducts-effectively-inhibit-foodborne-pathogens
#20
Shaimaa Hatab, Mei-Ling Chen, Wenhua Miao, Jiheng Lin, Dandan Wu, Changyu Wang, Pengxiang Yuan, Shanggui Deng
In this study, novel antimicrobial peptides had been derived by enzymatic hydrolysis of filefish (Thamnaconus modestus) byproduct (HFBP). Different proteases, (papain [P], trypsin [T], neutrase [N], pepsin [PE], and the mixture I [PN] and mixture II [PT]) and different hydrolysis time (60, 120, 180, and 240 min), have been used to generate peptides with different lengths and amino acid sequences. The antimicrobial properties of HFBPs were tested, against Gram-positive and Gram-negative strains, using disc diffusion method...
July 21, 2017: Foodborne Pathogens and Disease
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