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https://www.readbyqxmd.com/read/29453876/prognostic-importance-of-fgf2-and-fgfr1-expression-for-patients-affected-by-ameloblastoma
#1
Felipe Paiva Fonseca, Bernar Monteiro Benites, Ciro Dantas Soares, Thayná Melo de Lima Morais, Gleyson Kleber do Amaral-Silva, Oslei Paes de Almeida, Fernando Augusto Soares, Eduardo Rodrigues Fregnani
BACKGROUND: Fibroblast growth factor 2 (FGF2) and FGF receptor 1 (FGFR1) have been investigated in different human neoplasms and were shown to play important roles in the pathogenesis of these diseases; however, very few is known regarding their prognostic importance in the context of ameloblastoma. Therefore, the aim of this study is to investigate if the expression of FGF2 and FGFR1 is associated with ameloblastoma clinical behavior. METHODS: Fifty-eight cases of ameloblastoma arranged in tissue microarray were submitted to immunohistochemistry against FGF2 and FGFR1...
February 17, 2018: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29451216/does-combined-therapy-of-curcumin-and-epigallocatechin-gallate-have-a-synergistic-neuroprotective-effect-against-spinal-cord-injury
#2
Jiri Ruzicka, Lucia Machova Urdzikova, Barbora Svobodova, Anubhav G Amin, Kristyna Karova, Jana Dubisova, Kristyna Zaviskova, Sarka Kubinova, Meic Schmidt, Meena Jhanwar-Uniyal, Pavla Jendelova
Systematic inflammatory response after spinal cord injury (SCI) is one of the factors leading to lesion development and a profound degree of functional loss. Anti-inflammatory compounds, such as curcumin and epigallocatechin gallate (EGCG) are known for their neuroprotective effects. In this study, we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression. Immediately after SCI, rats received curcumin, EGCG, curcumin + EGCG or saline [daily intraperitoneal doses (curcumin, 6 mg/kg; EGCG 17 mg/kg)] and weekly intramuscular doses (curcumin, 60 mg/kg; EGCG 17 mg/kg)] for 28 days...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29446977/establishment-growth-proliferation-and-gene-expression-of-buffalo-bubalus-bubalis-transgenic-fetal-fibroblasts-containing-human-insulin-gene-and-production-of-embryos-by-handmade-cloning-using-these-cells
#3
Parul Mehta, Ramakant Kaushik, Karn Pratap Singh, Ankur Sharma, Manoj Kumar Singh, Manmohan Singh Chauhan, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik
The aim of the present study was to compare transgenic cells, containing human insulin gene kept under the control of mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic cells, for examining their potential for the production of embryos following somatic cell nuclear transfer (SCNT). The gene construct was delivered into buffalo fetal fibroblasts (BFF) by nucleofection following which, the transfected cells were selected by culture in the presence of G418 for 3 weeks...
February 15, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29440894/sustained-release-of-fgf-2-from-a-hybrid-hydrogel-of-heparin-poloxamer-and-decellular-matrix-promotes-the-neuroprotective-effects-of-proteins-after-spinal-injury
#4
He-Lin Xu, Fu-Rong Tian, Jian Xiao, Pian-Pian Chen, Jie Xu, Zi-Liang Fan, Jing-Jing Yang, Cui-Tao Lu, Ying-Zheng Zhao
Introduction: The short lifetime of protein-based therapies has largely limited their therapeutic efficacy in injured nervous post-spinal cord injury (post-SCI). Methods: In this study, an affinity-based hydrogel delivery system provided sustained-release of proteins, thereby extending the efficacy of such therapies. The affinity-based hydrogel was constructed using a novel polymer, heparin-poloxamer (HP), as a temperature-sensitive bulk matrix and decellular spinal cord extracellular matrix (dscECM) as an affinity depot of drug...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29438518/obesity-and-high-fat-diet-induce-distinct-changes-in-placental-gene-expression-and-pregnancy-outcome
#5
Erica B Mahany, Xingfa Han, Beatriz C Borges, Sanseray da Silveira Cruz-Machado, Susan J Allen, David Garcia-Galiano, Mark J Hoenerhoff, Nicole H Bellefontaine, Carol F Elias
Obese women are at high risk of pregnancy complications, including preeclampsia, miscarriage, preterm birth, stillbirth and neonatal death. In the present study, we aimed to determine the effects of obesity on pregnancy outcome and placental gene expression in preclinical mouse models of genetic and nutritional obesity. The leptin receptor null reactivatable (LepRloxTB), the LepR deficient (Leprdb/+) and high fat diet (HFD) fed mice were assessed for fertility, pregnancy outcome, placental morphology and placental transcriptome using standard qPCR and qPCR arrays...
February 9, 2018: Endocrinology
https://www.readbyqxmd.com/read/29436393/therapeutically-targeting-tumor-microenvironment-mediated-drug-resistance-in-estrogen-receptor-positive-breast-cancer
#6
Kevin Shee, Wei Yang, John W Hinds, Riley A Hampsch, Frederick S Varn, Nicole A Traphagen, Kishan Patel, Chao Cheng, Nicole P Jenkins, Arminja N Kettenbach, Eugene Demidenko, Philip Owens, Anthony C Faber, Todd R Golub, Ravid Straussman, Todd W Miller
Drug resistance to approved systemic therapies in estrogen receptor-positive (ER+) breast cancer remains common. We hypothesized that factors present in the human tumor microenvironment (TME) drive drug resistance. Screening of a library of recombinant secreted microenvironmental proteins revealed fibroblast growth factor 2 (FGF2) as a potent mediator of resistance to anti-estrogens, mTORC1 inhibition, and phosphatidylinositol 3-kinase inhibition in ER+ breast cancer. Phosphoproteomic analyses identified ERK1/2 as a major output of FGF2 signaling via FGF receptors (FGFRs), with consequent up-regulation of Cyclin D1 and down-regulation of Bim as mediators of drug resistance...
February 7, 2018: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29430457/evaluating-the-behavior-of-cultured-sertoli-cells-in-the-presence-and-absence-of-spermatogonial-stem-cell
#7
Masoome Jabarpour, Parviz Tajik
Background: The complex process of spermatogenesis is regulated by various factors. Several studies have been conducted to proliferate cells involved in the spermatogenesis process, in culture by used growth factors, different hormones and feeder cells. This study was conducted to evaluate the role of Sertoli cells on gene expression of fibroblast growth factor (FGF2) and glial cell derived neurotrophic factor (GDNF) after removal of spermatogonial stem cells (SSCs) from the culture medium...
2018: Stem Cell Investigation
https://www.readbyqxmd.com/read/29429958/human-ipsc-derived-posterior-gut-progenitors-are-expandable-and-capable-of-forming-gut-and-liver-organoids
#8
Ran-Ran Zhang, Masaru Koido, Tomomi Tadokoro, Rie Ouchi, Tatsuya Matsuno, Yasuharu Ueno, Keisuke Sekine, Takanori Takebe, Hideki Taniguchi
Early endoderm progenitors naturally possess robust propagating potential to develop a majority of meter-long gastrointestinal tracts and are therefore considered as a promising source for therapy. Here, we demonstrated the reproducible generation of human CDX2 + posterior gut endoderm cells (PGECs) from five induced pluripotent stem cell clones by manipulating FGF, TGF, and WNT signaling. Transcriptome analysis suggested that putative PGECs harbored an intermediate signature profile between definitive endoderm and organ-specific endoderm...
February 1, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29409666/growth-factors-fgf8-and-fgf2-and-their-receptor-fgfr1-transcriptional-factors-msx-1-and-msx-2-and-apoptotic-factors-p19-and-rip5-participate-in-the-early-human-limb-development
#9
Tina Becic, Darko Kero, Katarina Vukojevic, Snjezana Mardesic, Mirna Saraga-Babic
The expression pattern of fibroblast growth factors FGF8 and FGF2 and their receptor FGFR1, transcription factors MSX-1 and MSX-2, as well as cell proliferation (Ki-67) and cell death associated caspase-3, p19 and RIP5 factors were analyzed in histological sections of eight 4th-9th-weeks developing human limbs by immunohistochemistry and semi-thin sectioning. Increasing expression of all analyzed factors (except FGF8) characterized both the multilayered human apical ectodermal ridge (AER), sub-ridge mesenchyme (progress zone) and chondrocytes in developing human limbs...
February 3, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29408545/targeted-delivery-of-fgf2-to-subchondral-bone-enhanced-the-repair-of-articular-cartilage-defect
#10
Wenyu Yang, Yiting Cao, Zhe Zhang, Fuchong Du, Yanping Shi, Xuemin Li, Qiqing Zhang
It is reported that growth factor (GF) is able to enhance the repair of articular cartilage (AC) defect, however underlying mechanisms of which are not fully elucidated yet. Moreover, the strategy for delivering GF needs to be optimized. The crosstalk between AC and subchondral bone (SB) play important role in the homeostasis and integrity of AC, therefore SB targeted delivery of GF represents one promising way to facilitate the repair of AC defect. In this study, we firstly investigated the effects and mechanism of FGF2 on surrounding SB and cartilage of detect defects in rabbits by using a homogenous collagen-based membranes...
February 1, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29408210/chitosan-hydrogel-in-combination-with-marine-peptides-from-tilapia-for-burning-healing
#11
Qian-Qian Ouyang, Zhang Hu, Zhen-Peng Lin, Yi-Feng Deng, Wei-Yan Quan, Si-Dong Li, Peng-Zhi Hong, Pu-Wang Li, Cheng-Peng Li, Yu Chen, Ting-Ting Yan, Qing-Kun Zhong
The purpose of this study was to develop a promising burns dressing. Chiosan (CS) has been widely used as biomaterials, in combination with marine peptides (MPs) extracted from seawater cultured Tilapia, the newly developed material in this study (coded as CSMP) showed antibacterial activity, pro-cell proliferation and migration, well burning healing. Pathological examinations by HE staining demonstrated that CSMP had pronounced wound healing efficiencies. In burn wounds treated with CSMP, reepithelialization and collagen fiber deposition were observed on day 7, the epithelium was completely regenerated by day 14, and the wounds were completely healed by day 21...
February 2, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29402254/serum-and-synovial-fluid-cytokine-profiling-in-hip-osteoarthritis-distinct-from-knee-osteoarthritis-and-correlated-with-pain
#12
Guomin Ren, Ian Lutz, Pamela Railton, J Preston Wiley, Jenelle McAllister, James Powell, Roman J Krawetz
BACKGROUND: Inflammation is associated with the onset and progression of osteoarthritis in multiple joints. It is well known that mechanical properties differ between different joints, however, it remains unknown if the inflammatory process is similar/distinct in patients with hip vs. knee OA. Without complete understanding of the role of any specific cytokine in the inflammatory process, understanding the 'profile' of inflammation in a given patient population is an essential starting point...
February 5, 2018: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29397531/a-simplified-method-for-generating-purkinje-cells-from-human-induced-pluripotent-stem-cells
#13
Lauren M Watson, Maggie M K Wong, Jane Vowles, Sally A Cowley, Esther B E Becker
The establishment of a reliable model for the study of Purkinje cells in vitro is of particular importance, given their central role in cerebellar function and pathology. Recent advances in induced pluripotent stem cell (iPSC) technology offer the opportunity to generate multiple neuronal subtypes for study in vitro. However, to date, only a handful of studies have generated Purkinje cells from human pluripotent stem cells, with most of these protocols proving challenging to reproduce. Here, we describe a simplified method for the reproducible generation of Purkinje cells from human iPSCs...
February 3, 2018: Cerebellum
https://www.readbyqxmd.com/read/29392781/activated-receptor-tyrosine-kinases-in-granulosa-cells-of-ovulating-follicles-in-mice
#14
Yasmin Schuermann, Dayananda Siddappa, Melissa Pansera, Raj Duggavathi
Successful ovulation requires the actions of gonadotropins along with those mediated by growth factors binding to their receptor tyrosine kinases (RTKs). There are several growth factors such as epidermal growth factor family ligands and interleukins that play a role during ovulation initiated by the preovulatory surge of luteinizing hormone (LH). The aim of this project was to analyze growth factor signaling pathways induced by LH in mouse granulosa cells. Immature female mice were treated with equine chorionic gonadotropin (eCG) followed 48h later by human chorionic gonadotropin (hCG) to induce follicular growth and ovulation...
February 2, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29385487/microrna-221-expression-in-theca-and-granulosa-cells-hormonal-regulation-and-function
#15
C L Robinson, L Zhang, L F Schütz, M L Totty, L J Spicer
Small non-coding RNA molecules (miRNA) regulate protein levels in a post-transcriptional manner by partial base pairing to the 3'-UTR of target genes thus mediating degradation or translational repression. Previous studies indicate that numerous miRNA regulate the biosynthesis of intraovarian hormones, and emerging evidence indicates that one of these, miRNA-221 (MIR221), may be a modulator of ovarian function. However, the hormonal control of ovarian MIR221 is not known. The objectives of this study were to investigate the developmental and hormonal regulation of MIR221 expression in granulosa and theca cells and its possible role in regulating follicular function...
January 29, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29383184/stabilization-of-sur8-via-pkc%C3%AE-%C3%AE-degradation-promotes-transformation-and-migration-of-colorectal-cancer-cells
#16
Kug Hwa Lee, Woo-Jeong Jeong, Pu-Hyeon Cha, Sang-Kyu Lee, Do Sik Min, Kang-Yell Choi
Scaffold proteins of the mitogen activated protein kinase (MAPK) pathway recruit protein kinase cascades to confer context-specificity to cellular signaling. Varying concentrations of scaffold proteins determine different aspects of signaling outputs. However, regulatory mechanisms of scaffold proteins are poorly understood. Sur8, a scaffold protein in the Ras-MAPK pathway, is known to be involved in cell transformation and migration, and is increased in human colorectal cancer (CRC) patient tissue. Here we determine that regulation of Sur8 stability mediates transformation and migration of CRC cells...
December 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29382571/fgf2-and-fam201a-affect-the-development-of-osteonecrosis-of-the-femoral-head-after-femoral-neck-fracture
#17
Gangyong Huang, Guanglei Zhao, Jun Xia, Yibing Wei, Feiyan Chen, Jie Chen, Jingsheng Shi
Osteonecrosis of the femoral head (ONFH) is a common orthopedic disease associated with high disability, and femoral neck fracture (FNF) is one of the most common reasons for traumatic ONFH. This study was designed to reveal the mechanisms underlying ONFH. Using fastx_toolkit and prinseq-lite tools, quality control was conducted for the sequencing data. The differentially expressed genes (DEGs, including both mRNAs and lncRNAs) between ONFH and FNF samples were identified using the edgeR package in R, and were then subjected to enrichment analysis using the BioCloud platform...
January 27, 2018: Gene
https://www.readbyqxmd.com/read/29364044/linc-ram-is-required-for-fgf2-function-in-regulating-myogenic-cell-differentiation
#18
Yixia Zhao, Fengqi Cao, Xiaohua Yu, Chuyan Chen, Jiao Meng, Ran Zhong, Yong Zhang, Dahai Zhu
Myogenic differentiation of skeletal muscle stem cells, also known satellite cells, is tightly orchestrated by extrinsic and intrinsic regulators. Basic fibroblast growth factor (FGF2) is well documented to be implicated in satellite cell self-renewal and differentiation by repressing MyoD. We recently identified a MyoD-regulated and skeletal muscle-specifically expressed long non-coding RNA Linc-RAM which enhances myogenic differentiation by facilitating MyoD/Baf60c/Brg1 complex assembly. Herein, we investigated the transcriptional regulation and intracellular signaling pathway in mediating Linc-RAM gene expression during muscle cell differentiation...
January 24, 2018: RNA Biology
https://www.readbyqxmd.com/read/29363574/fibroblast-growth-factor-2-induces-proliferation-and-fibrosis-via-snai1-mediated-activation-of-cdk2-and-zeb1-in-corneal-endothelium
#19
JeongGoo Lee, Eric Jung, Martin Heur
Investigating stimulation of endogenous wound healing in corneal endothelial cells (CECs) may help address the global shortage of donor corneas by decreasing the number of transplants performed for blindness due to endothelial dysfunction.  We previously reported that IL-1β stimulation leads to fibroblast growth factor (FGF2) expression, enhancing migration and proliferation of mammalian CECs.  However, FGF2 also promotes the endothelial-mesenchymal transition, which can lead to retrocorneal membrane formation and blindness...
January 23, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29361536/fgf2-dependent-mesenchyme-and-laminin-111-are-niche-factors-in-salivary-gland-organoids
#20
Zeinab F Hosseini, Deirdre A Nelson, Nicholas Moskwa, Lauren M Sfakis, James Castracane, Melinda Larsen
Epithelial progenitor cells are dependent upon a complex 3D niche, which can be recapitulated in organoids. The specific requirements of the niche remain unclear for many cell types, including the proacinar cells that give rise to secretory acinar epithelial cells that produce saliva. Using ex vivo cultures of E16 primary mouse submandibular salivary gland epithelial cell clusters, we investigated the requirement for mesenchymal cells and other factors in producing salivary organoids in culture. Native E16 salivary mesenchyme, but not NIH3T3 cells or mesenchymal cell conditioned media, supported robust protein expression of the progenitor marker, Kit, and the acinar/proacinar marker, AQP5, with a requirement for FGF2 expression by the mesenchyme...
January 12, 2018: Journal of Cell Science
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