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https://www.readbyqxmd.com/read/28642358/epcr-a-novel-marker-of-cultured-cord-blood-hscs
#1
Gaëlle H Martin, Christopher Y Park
No abstract text is available yet for this article.
June 22, 2017: Blood
https://www.readbyqxmd.com/read/28603500/epcr-gene-ser219gly-polymorphism-and-venous-thromboembolism-a-meta-analysis-of-9-494-subjects
#2
Yan-Yan Li, Jing-Jing Wu, Xin-Xing Yang, Hong-Yu Geng, Ge Gong, Hyun Jun Kim
Background: Although endothelial cell protein C receptor (EPCR) gene Ser219Gly polymorphism has been associated with venous thromboembolism (VTE) susceptibility, no clear consensus has yet been reached. Objective and methods: A meta-analysis of 9,494 subjects from 13 individual studies was conducted to better elucidate the potential relationship between the EPCR gene Ser219Gly polymorphism and VTE. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs) were evaluated by using fixed or random effect models...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28538400/carbon-monoxide-releasing-molecule-2-suppresses-thrombomodulin-and-endothelial-protein-c-receptor-expression-of-human-umbilical-vein-endothelial-cells-induced-by-lipopolysaccharide-in-vitro
#3
Xianglin Meng, Dongsheng Fei, Mingming Liu, Songlin Yang, Ning Song, Lei Jiang, Kai Kang, Chuanchuan Nan, Yunpeng Luo, Shangha Pan, Mingyan Zhao
OBJECTIVE: The aim of this study was to observe the counter-effect of carbon monoxide-releasing molecule-2 (CORM-2) on lipopolysaccharide (LPS)-suppressed thrombomodulin (TM) and endothelial protein C receptor (EPCR) expressions from human umbilical vein endothelial cell (HUVEC), and to reveal its mechanisms. METHODS: HUVECs were divided into 5 treatment groups, wherein reagents were added simultaneously. TM and EPCR proteins of the cells and the culture medium levels of soluble TM, soluble EPCR, and matrix metalloproteinase-2 (MMP-2) were detected after administration, whereas mRNA levels of TM and EPCR, as well as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity among groups, were also evaluated...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28524456/endothelial-microparticles-released-by-activated-protein-c-protect-beta-cells-through-epcr-par1-and-annexin-a1-fpr2-pathways-in-islets
#4
Guillaume Kreutter, Mohamad Kassem, Ali El Habhab, Philippe Baltzinger, Malak Abbas, Julie Boisrame-Helms, Lamia Amoura, Jean Peluso, Blandine Yver, Zobairi Fatiha, Geneviève Ubeaud-Sequier, Laurence Kessler, Florence Toti
Islet transplantation is associated with early ischaemia/reperfusion, localized coagulation and redox-sensitive endothelial dysfunction. In animal models, islet cytoprotection by activated protein C (aPC) restores islet vascularization and protects graft function, suggesting that aPC triggers various lineages. aPC also prompts the release of endothelial MP that bear EPCR, its specific receptor. Microparticles (MP) are plasma membrane procoagulant vesicles, surrogate markers of stress and cellular effectors...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28522939/identification-of-protein-targets-in-cerebral-endothelial-cells-for-brain-arteriovenous-malformation-avms-molecular-therapies
#5
Margaret Simonian, Rachel R Ogorzalek Loo, Nalaka Rannulu, Joseph A Loo, Mark P Molloy, Marcus A Stoodley
BACKGROUND: To develop a new molecular targeted treatment for brain (AVMs), identification of membrane proteins that are localised on the AVM endothelium is crucial. Current treatment methods are surgery and radiosurgery. However, complete occlusion post radiosurgery are achieved within 3 years, while patient remain at risk of haemorrhage. This study aims to identify potential protein targets in AVM endothelial cells that discriminate these vessels from normal vessels; these proteins targets will be investigated for the molecular therapy of brain AVMs to promote rapid thrombosis after radiosurgery...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28480559/the-endothelial-protein-c-receptor-is-a-potential-stem-cell-marker-for-epidermal-keratinocytes
#6
Meilang Xue, Suat Dervish, Benjamin Chan, Christopher J Jackson
Endothelial protein C receptor (EPCR) is a specific receptor for anticoagulant protein C and expressed by human epidermis and cultured keratinocytes. Here we investigated whether: (a) the level of EPCR in keratinocytes is associated with their growth potential; and (b) EPCR is a potential marker for human epidermal stem cells. Human keratinocytes isolated from foreskins or adult skin tissues were transfected with EPCR siRNA or EPCR overexpressing plasmids. Cell proliferation, long term proliferation potential, colony forming efficiency (CFE), and in vitro epidermal regeneration ability of EPCR(high) and EPCR(l) °(w) cells were assessed...
May 7, 2017: Stem Cells
https://www.readbyqxmd.com/read/28448740/sequencing-on-the-solid-5500xl-system-in-depth-characterization-of-the-gc-bias
#7
Simone Roeh, Peter Weber, Monika Rex-Haffner, Jan M Deussing, Elisabeth B Binder, Mira Jakovcevski
Different types of sequencing biases have been described and subsequently improved for a variety of sequencing systems, mostly focusing on the widely used Illumina systems. Similar studies are missing for the SOLiD 5500xl system, a sequencer which produced many data sets available to researchers today. Describing and understanding the bias is important to accurately interpret and integrate these published data in various ongoing research projects. We report a particularly strong GC bias for this sequencing system when analyzing a defined gDNA mix of 5 microbes with a wide range of different GC contents (20-72%) when comparing to the expected distribution and Illumina MiSeq data from the same DNA pool...
April 27, 2017: Nucleus
https://www.readbyqxmd.com/read/28435976/-role-of-igg-plasma-cells-in-the-change-of-protein-c-system-in-ulcerative-colitis
#8
Xu-Hong Lin, Jun-Ling Guo, Yu-Qing Wen, Yu-Xia Li, Dan-Dan Wei, Rui-Lin Yang, Xiao-Yun Mu, Hui-Chao Wang
The present study is designed to explore the role of plasma cells in the change of protein C system (PCS) in ulcerative colitis (UC). Dextran sulfate sodium (DSS, 4% in concentration) was used to induce mouse UC model. The plasma cells and the type of immune complex in colon were observed by immunofluorescence. The amount and type of plasma cells separated from colonic mucosal lamina propria were detected by flow cytometry using anti-CD54(+)CD38(+) and IgA/M/G antibodies, respectively. After stimulation of macrophages by IgG type immune complex, TNF-α and IL-6 levels were evaluated by ELISA...
April 25, 2017: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/28415941/endothelial-protein-c-receptor-polymorphisms-and-risk-of-sepsis-in-a-chinese-population
#9
Yanbing Liang, Xia Huang, Yujie Jiang, Yueqiu Qin, Dingwei Peng, Yuqing Huang, Jin Li, Suren R Sooranna, Liao Pinhu
Objective To examine the potential relationship of EPCR polymorphisms and the risk of sepsis in a Chinese population. Methods Snapshot SNP genotyping assays and DNA sequencing methods were used to detect polymorphisms of the EPCR gene, rs2069948C/T (2532C/T) and rs867186A/G (6936A/G), in 64 patients with sepsis and in 113 controls. Soluble EPCR (sEPCR) was measured by ELISA. Results There were significant differences in the allele and genotype frequencies of EPCR gene rs2069948C/T and allele frequencies of rs867186A/G between male and female patients and controls...
April 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28408624/activated-protein-c-inhibits-neutrophil-extracellular-trap-formation-in-vitro-and-activation-in-vivo
#10
Laura D Healy, Cristina Puy, José A Fernández, Annachiara Mitrugno, Ravi S Keshari, Nyiawung A Taku, Tiffany T Chu, Xiao Xu, András Gruber, Florea Lupu, John H Griffin, Owen J T McCarty
Activated protein C (APC) is a multifunctional serine protease with anticoagulant, cytoprotective, and anti-inflammatory activities. In addition to the cytoprotective effects of APC on endothelial cells, podocytes, and neurons, APC cleaves and detoxifies extracellular histones, a major component of neutrophil extracellular traps (NETs). NETs promote pathogen clearance but also can lead to thrombosis; the pathways that negatively regulate NETosis are largely unknown. Thus, we studied whether APC is capable of directly inhibiting NETosis via receptor-mediated cell signaling mechanisms...
May 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28408459/epcr-expression-marks-um171-expanded-cd34-cord-blood-stem-cells
#11
Iman Fares, Jalila Chagraoui, Bernhard Lehnertz, Tara MacRae, Nadine Mayotte, Elisa Tomellini, Léo Aubert, Philippe P Roux, Guy Sauvageau
A small subset of human cord blood CD34(+) cells express endothelial protein c receptor (EPCR/CD201/PROCR) when exposed to hematopoietic stem cell (HSC) self-renewal agonist UM171. Here we show that EPCR-positive UM171-treated cells, as opposed to EPCR-negative cells, exhibit robust multi-lineage repopulation and serial reconstitution ability in immunocompromised mice. In contrast to other stem cell markers such as CD38, EPCR expression is maintained when cells are introduced in culture, irrespective of UM171 treatment...
April 13, 2017: Blood
https://www.readbyqxmd.com/read/28367652/astragaloside-iv-inhibits-pma-induced-epcr-shedding-through-mapks-and-pkc-pathway
#12
Yu Ma, Yi Zhao, Ran Zhang, Xiaoxia Liang, Zhongqiong Yin, Yi Geng, Gang Shu, Xu Song, Yuanfeng Zou, Lixia Li, Lizi Yin, Guizhou Yue, Yinglun Li, Gang Ye, Changliang He
Astragaloside IV (AS-IV), a main active substance isolated from Astragalus membranaceus Bunge, has been shown to have multiple pharmacological effects. Endothelial cell protein C receptor (EPCR) is a marker of inflammation, and is also a major member of protein C (PC) anti-coagulation system. EPCR can be cut off from the cell surface by tumor necrosis factor-α converting enzyme (TACE), which is controlled through mitogen-activated protein kinase (MAPK) and protein kinase C (PKC) pathways. To develop novel therapeutic drug for EPCR shedding, the effect of AS-IV was studied in phorbol-12-myristate 13-acetate (PMA)-induced human umbilical vein endothelial cells (HUVECs) and the potential molecular mechanism of AS-IV action was investigated...
April 3, 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28353616/associations-between-polymorphisms-in-coagulation-related-genes-and-venous-thromboembolism-a-meta-analysis-with-trial-sequential-analysis
#13
REVIEW
Jun Jiang, Kang Liu, Junjie Zou, Hao Ma, Hongyu Yang, Xiwei Zhang, Yuanyong Jiao
BACKGROUND: Recently, several studies showed that the polymorphisms in the coagulation-related genes might be associated with venous thromboembolism (VTE); however, the results were still controversial. We performed a meta-analysis with trial sequential analysis to investigate the associations between the endothelial cell-activated protein C receptor (EPCR) rs9574, F11 rs2289252, F11 rs2036914, FGG rs2066865, FGG rs1049636, CYP4V2 rs13146272, SERPINC1 rs2227589, and GP6 rs1613662 polymorphisms with the risk of VTE...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28279348/structure-guided-identification-of-a-family-of-dual-receptor-binding-pfemp1-that-is-associated-with-cerebral-malaria
#14
Frank Lennartz, Yvonne Adams, Anja Bengtsson, Rebecca W Olsen, Louise Turner, Nicaise T Ndam, Gertrude Ecklu-Mensah, Azizath Moussiliou, Michael F Ofori, Benoit Gamain, John P Lusingu, Jens E V Petersen, Christian W Wang, Sofia Nunes-Silva, Jakob S Jespersen, Clinton K Y Lau, Thor G Theander, Thomas Lavstsen, Lars Hviid, Matthew K Higgins, Anja T R Jensen
Cerebral malaria is a deadly outcome of infection by Plasmodium falciparum, occurring when parasite-infected erythrocytes accumulate in the brain. These erythrocytes display parasite proteins of the PfEMP1 family that bind various endothelial receptors. Despite the importance of cerebral malaria, a binding phenotype linked to its symptoms has not been identified. Here, we used structural biology to determine how a group of PfEMP1 proteins interacts with intercellular adhesion molecule 1 (ICAM-1), allowing us to predict binders from a specific sequence motif alone...
March 8, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28263407/bartonella-henselae-in-canine-cavitary-effusions-prevalence-identification-and-clinical-associations
#15
Amy L Weeden, Natalie A Cherry, Edward B Breitschwerdt, Avery G Cheves, Heather L Wamsley
BACKGROUND: Previous reports suggest an association between Bartonella infection and effusions in dogs and human beings. OBJECTIVES: The aims of this study were to determine the prevalence of Bartonella infection in canine effusions and to investigate historic and clinical parameters predictive of Bartonella in dogs with effusions. METHODS: Canine cavitary effusions submitted for analysis and, if available, paired EDTA blood, were screened for Bartonella infection using the Bartonella α-proteobacteria growth medium enrichment culture/PCR diagnostic platform (Bartonella enrichment PCR or ePCR) at Galaxy Diagnostics, Inc...
June 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28223242/fviia-prevents-the-progressive-hemorrhaging-of-a-brain-contusion-by-protecting-microvessels-via-formation-of-the-tf-fviia-fxa-complex
#16
Qiang Yuan, Dalong Zhang, Sirong Wu, Jian Yu, Lei Yu, Yirui Sun, Zhuoying Du, Zhiqi Li, Liangfu Zhou, Xing Wu, Jin Hu
Factor VII (FVII) plays a key role in the initiation of the coagulation cascade and, in clinical situations, recombinant human activated FVII (rFVIIa) effectively prevents progressive hemorrhaging after a brain contusion. However, it remains unclear whether decreases in FVII activity directly lead to progressive hemorrhaging and, moreover, the precise mechanisms underlying this process are not yet known. The present study demonstrated that decreased FVII activity directly led to progressive hemorrhaging of the cerebral contusions...
February 20, 2017: Neuroscience
https://www.readbyqxmd.com/read/28219843/the-impact-of-the-endothelial-protein-c-receptor-on-thrombin-generation-and-clot-lysis
#17
Laura Pepler, Chengliang Wu, Dhruva J Dwivedi, Cynthia Wu, Paul Y Kim, Patricia C Liaw
INTRODUCTION: When thrombin is bound to thrombomodulin (TM), it becomes a potent activator of protein C (PC) and thrombin-activable fibrinolysis inhibitor (TAFI). Activation of PC is enhanced when PC is bound to the endothelial protein C receptor (EPCR). Activated protein C (APC) inhibits thrombin generation while activated TAFI (TAFIa) attenuates fibrinolysis. To determine the impact of diminished EPCR function on thrombin generation and fibrinolysis we generated cells that expressed TM and a variant of EPCR (R96C) that does not bind PC...
April 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28140640/endothelial-protein-c-receptor-dependent-antichemotactic-effects-of-canine-protein-c
#18
Valerie M Wong, Olivier Côté, Dorothee Bienzle, M Anthony Hayes, R Darren Wood
OBJECTIVE To determine whether canine protein C (CnPC) had antichemotactic effects on canine neutrophils, whether endothelial protein C receptor (EPCR) was expressed on canine neutrophils, and the role of EPCR in neutrophil chemotaxis. SAMPLE Neutrophils isolated from blood samples from healthy dogs (n = 6) and sick dogs with (2) or without (3) an inflammatory leukogram. PROCEDURES Neutrophils were analyzed by reverse transcriptase PCR assay and flow cytometry for detection of EPCR mRNA and protein expression, respectively...
February 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28138022/the-severity-of-plasmodium-falciparum-infection-is-associated-with-transcript-levels-of-var-genes-encoding-endothelial-protein-c-receptor-binding-p-falciparum-erythrocyte-membrane-protein-1
#19
Sixbert I Mkumbaye, Christian W Wang, Eric Lyimo, Jakob S Jespersen, Alphaxard Manjurano, Jacklin Mosha, Reginald A Kavishe, Steven B Mwakalinga, Daniel T R Minja, John P Lusingu, Thor G Theander, Thomas Lavstsen
By attaching infected erythrocytes to the vascular lining, Plasmodium falciparum parasites leave blood circulation and avoid splenic clearance. This sequestration is central to pathogenesis. Severe malaria is associated with parasites expressing an antigenically distinct P. falciparum erythrocyte membrane protein 1 (PfEMP1) subset mediating binding to endothelial receptors. Previous studies indicate that PfEMP1 adhesins with so-called CIDRα1 domains capable of binding endothelial protein C receptor (EPCR) constitute the PfEMP1 subset associated with severe pediatric malaria...
April 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28103946/epcr-promotes-breast-cancer-progression-by-altering-spock1-testican-1-mediated-3d-growth
#20
Naiara Perurena, Carolina Zandueta, Susana Martínez-Canarias, Haritz Moreno, Silvestre Vicent, Ana S Almeida, Elisabet Guruceaga, Roger R Gomis, Marta Santisteban, Mikala Egeblad, José Hermida, Fernando Lecanda
BACKGROUND: Activated protein C/endothelial protein C receptor (APC/EPCR) axis is physiologically involved in anticoagulant and cytoprotective activities in endothelial cells. Emerging evidence indicates that EPCR also plays a role in breast stemness and human tumorigenesis. Yet, its contribution to breast cancer progression and metastasis has not been elucidated. METHODS: Transcriptomic status of EPCR was examined in a cohort of 286 breast cancer patients. Cell growth kinetics was evaluated in control and EPCR and SPARC/osteonectin, Cwcv, and kazal-like domains proteoglycan (SPOCK1/testican 1) silenced breast cancer cells in 2D, 3D, and in co-culture conditions...
January 19, 2017: Journal of Hematology & Oncology
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