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Linpo Liu, Ying Jiang, Xiaomei Zhang, Xu Wang, Yanbing Wang, Yuzhen Han, George Coupland, Jing Bo Jin, Iain R Searle, Yongfu Fu, Fulu Chen
In plants, the post-translational modification small ubiquitin-like modifier (SUMO) is involved in regulating several important developmental and cellular processes, including flowering-time control and responses to biotic and abiotic stresses. Here, we report two proteases, SUMO PROTEASE RELATED TO FERTILITY (SPF) 1 and SPF2, that regulate male and female gamete and embryo development and remove SUMO from proteins in vitro and in vivo. spf1 mutants exhibit abnormal floral structures and embryo development, while spf2 mutants exhibit largely a wild type phenotype...
October 24, 2017: Plant Physiology
Jie Wang, Xiao-Guan Zhu, Sheng-Hua Ying, Ming-Guang Feng
A global insight into the roles of multiple P-type calcium ATPase (CA) pumps in sustaining the life of a filamentous fungal pathogen is lacking. Here we elucidated the functions of five CA pumps (Eca1, Spf1 and PmcA/B/C) following previous characterization of Pmr1 in Beauveria bassiana, a fungal insect pathogen. The fungal CA pumps interacted at transcriptional level, at which singular deletions of five CA genes depressed eca1 expression by 76-98% and deletion of spf1 resulted in drastic upregulation of four CA genes by 36-50-fold...
May 3, 2017: Scientific Reports
Fufen Yin, Ning Wang, Shanshan Wang, Fengsheng Yu, Xin Sun, Xiao Yu, Bing Luo, Chengquan Zhao, Yankui Wang
Both human papillomavirus (HPV) infection and the aberrant Ras associated domain family gene 1A (RASSF1A) promoter methylation status participate in the pathogenesis of cervical cancer. Some studies suggest that E6, and E7 are involved in the pathogenetic mechanisms of RASSF1A. We mainly explored a possible involvement of HPV16 oncogenes E6 or/and E7 in RASSF1A promoter methylation status and possible roles of RASSF1A gene methylation in cervical cancer. Bisulfite genomic sequencing (BGS) PCR combined with TA clone, methylation-specific PCR (MSP) were used to analyze methylation status of the RASSF1A gene promoter in HPV16/18-positive and HPV-negative cervical cancer cell lines; ectopically expressed HPV16 E6, E7 and E6/E7 cervical cancer cell lines; normal cervical and cervical cancer tissues...
April 2017: Oncology Reports
Ling Zhu, Ruijiao Xin, Enamul Huq
Plants perceive red and far-red region of the light spectrum to regulate photomorphogenesis through a family of photoreceptors called phytochromes. Phytochromes transduce the light signals to trigger a cascade of downstream gene regulation in part via a subfamily of bHLH transcription factors called Phytochrome Interacting Factors (PIFs). As the repressors of light signaling pathways, most PIFs are phosphorylated and degraded through the ubiquitin/26S proteasome pathway in response to light. The mechanisms involved in the phosphorylation and degradation of PIFs have not been fully understood yet...
2016: Frontiers in Plant Science
Yuanwei Zhang, Qingqing Zheng, Congcong Sun, Jinxing Song, Lina Gao, Shizhu Zhang, Alberto Muñoz, Nick D Read, Ling Lu
Finely tuned changes in cytosolic free calcium ([Ca2+]c) mediate numerous intracellular functions resulting in the activation or inactivation of a series of target proteins. Palmitoylation is a reversible post-translational modification involved in membrane protein trafficking between membranes and in their functional modulation. However, studies on the relationship between palmitoylation and calcium signaling have been limited. Here, we demonstrate that the yeast palmitoyl transferase ScAkr1p homolog, AkrA in Aspergillus nidulans, regulates [Ca2+]c homeostasis...
April 2016: PLoS Genetics
Qiuju Deng, Jingjing Li, Yaqi Pan, Fangfang Liu, Zhonghu He, Mengfei Liu, Ying Liu, Chanyuan Zhang, Amir Abliz, Na Shen, Dong Hang, Zhongyao Xu, Qiyan Wang, Tao Ning, Chuanhai Guo, Yongmei Liang, Ruiping Xu, Lixin Zhang, Hong Cai, Yang Ke
Skin infections with cutaneous human papillomavirus (HPV) have been linked to the development of non-melanoma skin cancer, in which mucosal HPV may also play a crucial role. However, systematic investigations of the distribution and associated factors of HPV infection in healthy skin of the general population are scarce. HPV DNA from palmar exfoliated cells of 2,087 individuals was detected by FAP6085/64 and SPF1/GP6+ primers followed by sequencing. A total of 338 papillomavirus types were detected, with HPV-3, HPV-57, and HPV-49 being the most dominant types...
June 2016: Journal of Investigative Dermatology
Abdullah Abdullah, Muhammad Qasim, Muhammad Shafiq, Muhammad Ijaz, Shamsa Parveen, Shahnaz Murtaza, Qamar Javed, Salman Akbar Malik, Shahida Husain Tarar, Sajid Mehmood, Abdul Sami, Syed Muhammad Saqlan Naqvi, Muhammad Zeeshan Hyder
BACKGROUND: Human Papillomavirus (HPV) is well known pathogen that can cause benign and malignant tumors in humans, yet there is very little information regarding HPV types prevalent in Pakistan. METHODS: A total of 92 cervical secretions were collected from suspected married female patients and used for DNA isolation using a novel isolation method. The samples were tested through Polymerase Chain Reaction (PCR) using already reported primers MY09/MY11, GP5/GP6, GP5+/GP6+, CP65/CP70, CP66/CP69 and SPF1/SPF2 and with those developed in this study including HRT1 and HRT2 primer sets for typing HPV types and HACTB primer set for human beta actin gene as internal positive control...
2016: Infectious Agents and Cancer
Néstor García-Rodríguez, Javier Manzano-López, Miguel Muñoz-Bravo, Elisabet Fernández-García, Manuel Muñiz, Ralf Erik Wellinger
Regulation of intracellular ion homeostasis is essential for eukaryotic cell physiology. An example is provided by loss of ATP2C1 function, which leads to skin ulceration, improper keratinocyte adhesion, and cancer formation in Hailey-Hailey patients. The yeast ATP2C1 orthologue PMR1 codes for a Mn(2+)/Ca(2+) transporter that is crucial for cis-Golgi manganese supply. Here, we present evidence that calcium overcomes the lack of Pmr1 through vesicle trafficking-stimulated manganese delivery and requires the endoplasmic reticulum Mn(2+) transporter Spf1 and the late endosome/trans-Golgi Nramp metal transporter Smf2...
April 10, 2015: Journal of Biological Chemistry
Qilin Yu, Chang Jia, Yijie Dong, Bing Zhang, Chenpeng Xiao, Yulu Chen, Yuzhou Wang, Xiaoling Li, Lei Wang, Biao Zhang, Mingchun Li
Autophagy is a degradation process involved in pathogenicity of many pathogenic fungi. However, its roles in Candida albicans, the leading fungal pathogen in human beings, remain to be detailed. Most recently, we found that endoplasmic reticulum (ER) stress-inducing conditions led to transcriptional up-regulation of C. albicans autophagy-related (ATG) genes, implying a possible link between autophagy and ER stress response in this pathogen. Using a series of C. albicans ATG mutants and autophagy reporting systems, we found that both treatment of ER stress-related drugs and loss of the ER calcium pump Spf1 promoted autophagic flux of Atg8 and Lap41 (a homologue of Saccharomyces cerevisiae Ape1), indicating that these conditions induce autophagy...
August 2015: Fungal Genetics and Biology: FG & B
Dong Hang, Fangfang Liu, Mengfei Liu, Zhonghu He, Min Sun, Ying Liu, Jingjing Li, Yaqi Pan, Tao Ning, Chuanhai Guo, Yongmei Liang, Ruiping Xu, Lixin Zhang, Hong Cai, Yang Ke
BACKGROUND: Despite the established link between oral human papillomavirus (HPV) infection and a subset of oropharyngeal squamous cell carcinoma (OSCC), little is known about the epidemiology of oral HPV infection among healthy adults in China. METHODS: Oral swab specimens and questionnaires were collected from 5,410 individuals (ages 25-65 years). HPV DNA in oral exfoliated cells was tested by general primer-mediated (SPF1/GP6+) PCR and sequencing. Univariate and multivariate analyses were performed to assess the associations between exposure factors and oral infection...
October 2014: Cancer Epidemiology, Biomarkers & Prevention
Yifat Cohen, Márton Megyeri, Oscar C W Chen, Giuseppe Condomitti, Isabelle Riezman, Ursula Loizides-Mangold, Alaa Abdul-Sada, Nitzan Rimon, Howard Riezman, Frances M Platt, Anthony H Futerman, Maya Schuldiner
The endoplasmic reticulum (ER) is a large, multifunctional and essential organelle. Despite intense research, the function of more than a third of ER proteins remains unknown even in the well-studied model organism Saccharomyces cerevisiae. One such protein is Spf1, which is a highly conserved, ER localized, putative P-type ATPase. Deletion of SPF1 causes a wide variety of phenotypes including severe ER stress suggesting that this protein is essential for the normal function of the ER. The closest homologue of Spf1 is the vacuolar P-type ATPase Ypk9 that influences Mn(2+) homeostasis...
2013: PloS One
Rui Liu, Lingling Wang, Ying Sun, Leilei Wang, Huan Zhang, Linsheng Song
Low-density lipoprotein receptor-related protein (LRP) is a group of important endocytic receptors contributing to binding ligands and maintaining internal environment. In the present study, an LRP-like molecule was identified from Zhikong scallop Chlamys farreri (CfLPR), and its mRNA expression profiles, tissue location, and immunology activities were analyzed to explore its possible function in the innate immune system. The ORF of CfLRP was of 1971 bp encoding a polypeptide of 656 amino acids with ten low-density lipoprotein-receptor YWTD (LY) domains and one scavenger receptor cysteine-rich (SRCR) domain...
February 2014: Fish & Shellfish Immunology
Qilin Yu, Xiaohui Ding, Bing Zhang, Ning Xu, Xinxin Cheng, Kefan Qian, Biao Zhang, Laijun Xing, Mingchun Li
Endoplasmic reticulum (ER) is crucial for protein folding, glycosylation and secretion in eukaryotic organisms. These important functions are supported by high levels of Ca(2+) in the ER. We have recently identified a putative ER Ca(2+) pump in Candida albicans, called Spf1, which plays key roles in maintenance of cellular Ca(2+) homeostasis, morphogenesis and virulence. In this study, we purified Spf1 and confirmed that it is a P-type ATPase, suggesting its role in maintaining high levels of ER Ca(2+). Disruption of SPF1 caused severe defects in glycosylation of the ER-localized protein Cdc101 and secretory acid phosphatase, and a decrease in expression of SEC61 which encodes an important ER protein...
July 2013: International Journal of Medical Microbiology: IJMM
Yingjun Chi, Yan Yang, Yuan Zhou, Jie Zhou, Baofang Fan, Jing-Quan Yu, Zhixiang Chen
It has been almost 20 years since the first report of a WRKY transcription factor, SPF1, from sweet potato. Great progress has been made since then in establishing the diverse biological roles of WRKY transcription factors in plant growth, development, and responses to biotic and abiotic stress. Despite the functional diversity, almost all analyzed WRKY proteins recognize the TTGACC/T W-box sequences and, therefore, mechanisms other than mere recognition of the core W-box promoter elements are necessary to achieve the regulatory specificity of WRKY transcription factors...
March 2013: Molecular Plant
Katrin Krumpe, Idan Frumkin, Yonatan Herzig, Nitzan Rimon, Cagakan Özbalci, Britta Brügger, Doron Rapaport, Maya Schuldiner
Tail-anchored (TA) proteins have a single C-terminal transmembrane domain, making their biogenesis dependent on posttranslational translocation. Despite their importance, no dedicated insertion machinery has been uncovered for mitochondrial outer membrane (MOM) TA proteins. To decipher the molecular mechanisms guiding MOM TA protein insertion, we performed two independent systematic microscopic screens in which we visualized the localization of model MOM TA proteins on the background of mutants in all yeast genes...
October 2012: Molecular Biology of the Cell
Poulami Das, Asha Thomas, Umesh Mahantshetty, Shyam K Shrivastava, Kedar Deodhar, Rita Mulherkar
Persistent HPV infection plays a major role in cervical cancer. This study was undertaken to identify HPV types in a cohort of Indian women with locally advanced cervical cancer as well as to determine the physical state and/or site of viral integration in the host genome. Pretreatment biopsies (n = 270) from patients were screened for HPV infection by a high throughput HPV genotyping assay based on luminex xMAP technology as well as MY09/11 PCR and SPF1/2 PCR. Overall HPV positivity was observed to be 95%, with HPV16 being most common (63%) followed by infection with HPV18...
2012: PloS One
Qilin Yu, Hui Wang, Ning Xu, Xinxin Cheng, Yuzhou Wang, Biao Zhang, Laijun Xing, Mingchun Li
The maintenance of cellular calcium homeostasis is associated with cellular signalling transduction and the functions of many membrane compartments, especially endoplasmic reticulum (ER) function. ER-localized proteins that serve to maintain ER and cellular calcium homeostasis in Candida albicans are still unclear. In this study, Spf1, the putative C. albicans homologue of the Saccharomyces cerevisiae ER-localized P-type calcium ATPase ScSpf1, was investigated for its roles in cellular calcium homeostasis, hyphal development and virulence...
September 2012: Microbiology
Gerardo R Corradi, Felicitas de Tezanos Pinto, Luciana R Mazzitelli, Hugo P Adamo
The P5-ATPases are important components of eukaryotic cells. They have been shown to influence protein biogenesis, folding, and transport. The knowledge of their biochemical properties is, however, limited, and the transported ions are still unknown. We expressed in Saccharomyces cerevisiae the yeast Spf1 P5A-ATPase containing the GFP fused at the N-terminal end. The GFP-Spf1 protein was localized in the yeast endoplasmic reticulum. Purified preparations of GFP-Spf1 hydrolyzed ATP at a rate of ~0.3-1 μmol of P(i)/mg/min and formed a phosphoenzyme in a simple reaction medium containing no added metal ions except Mg(2+)...
August 31, 2012: Journal of Biological Chemistry
Fangcen Guo, Ying Liu, Xueqian Wang, Zhonghu He, Noel S Weiss, Margaret M Madeleine, Fangfang Liu, Xiuyun Tian, Yuqin Song, Yaqi Pan, Tao Ning, Haijun Yang, Xiaotian Shi, Changdong Lu, Hong Cai, Yang Ke
BACKGROUND: The risk factors for esophageal squamous cell carcinoma (ESCC) in the high-incidence areas of China remain unclear. METHODS: A total of 300 patients with ESCC and 900 controls matched for age and sex were enrolled in Anyang (China), a high-risk area for ESCC in China. In tumor tissue of the cases and in esophageal biopsies of controls, the presence of human papillomavirus (HPV) DNA was assessed by an SPF1/GP6(+)-mediated PCR followed by sequencing. The presence of serum antibody against the HPV-16 E7 oncoprotein was assessed by use of the ELISA...
May 2012: Cancer Epidemiology, Biomarkers & Prevention
Tuan Anh Pham, Shigeyuki Kawai, Emi Kono, Kousaku Murata
Transformation is an indispensable method for the manipulation of Saccharomyces cerevisiae cell. The spf1 cell, in which the gene encoding an endoplasmic reticulum-located P-type ATPase is deleted, has been known to show the high-transformation phenotype. In this study, fluorescent microscopic observation of transformation process of S. cerevisiae using plasmid DNA labelled with fluorescent DNA probe, YOYO-1, suggested that the spf1 cell absorbed more plasmid DNA on cellular surface than did the wild-type cell and the unwashed cell did more plasmid DNA than the washed cell...
March 2011: Current Microbiology
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