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https://www.readbyqxmd.com/read/28738515/characterization-of-carbon-dioxide-concentrating-chemolithotrophic-bacterium-serratia-sp-istd04-for-production-of-biodiesel
#1
Manish Kumar, Raj Morya, Edgard Gnansounou, Christian Larroche, Indu Shekhar Thakur
Proteomics and metabolomics analysis has become a powerful tool for characterization of microbial ability for fixation of Carbon dioxide. Bacterial community of palaeoproterozoic metasediments was enriched in the shake flask culture in the presence of NaHCO3. One of the isolate showed resistance to NaHCO3 (100mM) and was identified as Serratia sp. ISTD04 by 16S rRNA sequence analysis. Carbon dioxide fixing ability of the bacterium was established by carbonic anhydrase enzyme assay along with proteomic analysis by LC-MS/MS...
July 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28738269/an-anti-inflammatory-effect-of-red-microalga-polysaccharides-in-coronary-artery-endothelial-cells
#2
Oshrat Levy-Ontman, Mahmoud Huleihel, Rachel Hamias, Talya Wolak, Esther Paran
BACKGROUND AND AIMS: Polysaccharides (PSs) produced by the red microalga Porphyridium sp. were reported to exhibit anti-inflammatory bioactivities in the human skin. The primary goal of the present research was to assess whether PSs attenuate inflammatory processes by interfering with tumour necrosis factor-alpha (TNF-α)-induced inflammation, in human coronary artery endothelial cells (HCAECs). METHODS: Functional and inflammatory markers were quantified in TNF-α-stimulated HCAECs, with and without pre-treatment with PSs...
July 16, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28738150/nickel-catalyzed-%C3%AE-%C3%AE-dicarbofunctionalization-of-alkenyl-carbonyl-compounds-via-conjunctive-cross-coupling
#3
Joseph Derosa, Van Tran, Mark N Boulous, Jason S Chen, Keary M Engle
A nickel-catalyzed conjunctive cross-coupling between non-conjugated alkenes, aryl iodides, and alkylzinc reagents is reported. Excellent regiocontrol is achieved utilizing an 8-aminoquinoline directing group that can be readily cleaved to unmask net β,γ-dicarbofunctionalized carboxylic acid products. Under optimized conditions, both terminal and internal alkene substrates provided the corresponding alkyl/aryl difunctionalized products in moderate to excellent yields. The methodology developed herein represents the first 1,2-dicarbofunctionalization of non-conjugated alkenes involving a three-component C(sp(3))-C(sp(3)) cross-coupling reaction...
July 24, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28737929/enhanced-degradation-of-tce-on-a-superfund-site-using-endophyte-assisted-poplar-tree-phytoremediation
#4
Sharon Lafferty Doty, John Leonard Freeman, Christopher M Cohu, Joel Gerard Burken, Andrea Firrincieli, Andrew Simon, Zareen Khan, Jud G Isebrands, Joseph Lukas, Michael J Blaylock
Trichloroethylene (TCE) is a widespread environmental pollutant common in groundwater plumes associated with industrial manufacturing areas. We had previously isolated and characterized a natural bacterial endophyte, Enterobacter sp. strain PDN3, of poplar trees, that rapidly metabolizes TCE, releasing chloride ion. We now report findings from a successful three-year field trial of endophyte-assisted phytoremediation on the Middlefield-Ellis-Whisman Superfund Study Area TCE plume in the Silicon Valley of California...
July 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28737184/a-switchable-electrochromism-and-electrochemiluminescence-bifunctional-sensor-based-on-the-electro-triggered-isomerization-of-spiropyran-layered-double-hydroxides
#5
Zhixiong Li, Yuqiong Zhou, Liuqi Peng, Dongpeng Yan, Min Wei
A bifunctional ultrathin film (UTF) electrode was fabricated based on layered double hydroxides (LDHs) and spiropyran (SP), which exhibited electrochromism and electrochemiluminescence (ECL) sensing behavior toward temperature and Zn(2+) ions.
July 24, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28737179/total-synthesis-of-the-dictyodendrins-as-an-arena-to-highlight-emerging-synthetic-technologies
#6
REVIEW
Wenhan Zhang, Joseph M Ready
Covering: 1993-2017This review discusses the isolation, biological activity, and syntheses of the dictyodendrin class of natural products, covering the years 1993-2017. The dictyodendrins are a family of alkaloids isolated from marine sponges, Dictyodendrilla verongiformis and Ianthella sp., which possess a highly substituted pyrrolo[2,3-c]carbazole core at the phenol or quinone oxidation states. Dictyodendrins exhibit a wide range of biological activities, such as telomerase inhibition, BACE1 enzyme inhibition, and cytotoxicity against several cancer cell lines...
July 24, 2017: Natural Product Reports
https://www.readbyqxmd.com/read/28736925/engineering-a-bzd-cassette-for-the-anaerobic-bioconversion-of-aromatic-compounds
#7
María Teresa Zamarro, María J L Barragán, Manuel Carmona, José Luis García, Eduardo Díaz
Microorganisms able to degrade aromatic contaminants constitute potential valuable biocatalysts to deal with a significant reusable carbon fraction suitable for eco-efficient valorization processes. Metabolic engineering of anaerobic pathways for degradation and recycling of aromatic compounds is an almost unexplored field. In this work, we present the construction of a functional bzd cassette encoding the benzoyl-CoA central pathway for the anaerobic degradation of benzoate. The bzd cassette has been used to expand the ability of some denitrifying bacteria to use benzoate as sole carbon source under anaerobic conditions, and it paves the way for future pathway engineering of efficient anaerobic biodegraders of aromatic compounds whose degradation generates benzoyl-CoA as central intermediate...
July 24, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28736815/a-widespread-cyanobacterium-supported-by-polyphasic-approach-proposition-of-koinonema-pervagatum-gen-sp-nov-oscillatoriales
#8
Bruna Buch, Mariéllen Dornelles Martins, Luis Henrique Z Branco
Several new genera originally classified as the genus Phormidium, a polyphyletic and taxonomically complex genus within the Oscillatoriales, were recently described. The simple morphology of Phormidium does not reflect its genetic diversity and the delimitation of a natural group is not possible with traditional classification systems based on morphology alone. Therefore, this study used morphological, ecological and molecular approaches to evaluate four populations morphologically similar to Ammassolinea, Kamptonema and Ancylothrix (simple, curved and gradually attenuated at the ends trichome), found in subtropical and tropical Brazilian regions...
July 24, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28736770/classification-of-helpful-comments-on-online-suicide-watch-forums
#9
Ramakanth Kavuluru, Amanda G Williams, María Ramos-Morales, Laura Haye, Tara Holaday, Julie Cerel
Among social media websites, Reddit has emerged as a widely used online message board for focused mental health topics including depression, addiction, and suicide watch (SW). In particular, the SW community/subreddit has nearly 40,000 subscribers and 13 human moderators who monitor for abusive comments among other things. Given comments on posts from users expressing suicidal thoughts can be written from any part of the world at any time, moderating in a timely manner can be tedious. Furthermore, Reddit's default comment ranking does not involve aspects that relate to the "helpfulness" of a comment from a suicide prevention (SP) perspective...
October 2016: ACM-BCB: ACM Conference on Bioinformatics, Computational Biology and Biomedicine
https://www.readbyqxmd.com/read/28736724/biodecolorization-of-textile-azo-dye-using-bacillus-sp-strain-ch12-isolated-from-alkaline-lake
#10
Awoke Guadie, Samson Tizazu, Meseretu Melese, Wenshan Guo, Huu Hao Ngo, Siqing Xia
Textile azo dye decolorizing bacteria were isolated from alkaline Lakes Abaya and Chamo using Reactive Red 239 (RR239) dye. Through subsequent screening process, strain CH12 was selected to investigate the effects of nutrient supplement, DO, pH, temperature, dye concentration and types on decolorization. Based on 16S rRNA gene sequence analysis, strain CH12 was identified as Bacillus sp. Decolorization efficiencies were significantly enhanced with carbon (≥98%) and organic nitrogen (∼100%) supplements. Complete decolorization was also observed under anoxic and anaerobic conditions, and at the temperature of 30 °C and the pH of 10...
September 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28736549/cloning-expression-isotope-labeling-and-purification-of-transmembrane-protein-merf-from-mercury-resistant-enterobacter-sp-az-15-for-nmr-studies
#11
Aatif Amin, Zakia Latif
Mercury resistant (Hg(R)) Enterobacter sp. AZ-15 was isolated from heavy metal polluted industrial wastewater samples near to districts Kasur and Sheikhupura, Pakistan. 16S rDNA ribotyping and phylogentic analysis showed 98% homology with already reported Enterobacter species. The merF gene encoding transmembrane protein-MerF was amplified from genomic DNA and ligated into pET31b+ vector using restriction endonucleases, SphI and XhoI. The genetic codons of merF gene encoding cysteine residues were mutated into codons, translating into serine residues by site-directed mutagenesis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28736501/design-of-radioiodinated-pharmaceuticals-structural-features-affecting-metabolic-stability-towards-in-vivo-deiodination
#12
REVIEW
Lorenzo Cavina, Dion van der Born, Peter H M Klaren, Martin C Feiters, Otto C Boerman, Floris P J T Rutjes
Radioiodinated pharmaceuticals are convenient tracers for clinical and research investigations because of the relatively long half-lives of radioactive iodine isotopes (i.e., (123)I, (124)I, and (131)I) and the ease of their chemical insertion. Their application in radionuclide imaging and therapy may, however, be hampered by poor in vivo stability of the C-I bond. After an overview of the use of iodine in biology and nuclear medicine, we present here a survey of the catabolic pathways for iodinated xenobiotics, including their biodistribution, accumulation, and biostability...
June 30, 2017: European Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28736352/the-effect-of-biocomposite-screws-on-bone-regeneration-in-a-rat-osteoporosis-model
#13
Je Hoon Jeong, Eun-Sun Jin, Ji Yeon Kim, Bora Lee, JoongKee Min, Sang Ryong Jeon, Minsu Lee, Kyoung Hyo Choi
INTRODUCTION: The aim of this study was to evaluate the efficacy of biocomposite screws used in ovariectomy (OVX) induced osteoporotic rats. METHODS: Twenty-four female Wistar rats (250-300g, 12 weeks old) were divided into 4 groups; Sham group (control), OVX-induced osteoporosis group (OVX), OVX and biodegradable (PLGA (poly-lactic-co-glycolic acid) without ß-TCP (tricalcium phosphate) screw insertion group (OVX/BSR), OVX and biocomposite (PLGA with ß-TCP) screw insertion group (OVX/CSR)...
July 20, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28736103/what-impact-do-chaplains-have-a-pilot-study-of-spiritual-aim-for-advanced-cancer-patients-in-outpatient-palliative-care
#14
Allison Kestenbaum, Michele Shields, Jennifer James, Will Hocker, Stefana Morgan, Shweta Karve, Michael W Rabow, Laura B Dunn
CONTEXT: Spiritual care is integral to quality palliative care. Although chaplains are uniquely trained to provide spiritual care, studies evaluating chaplains' work in palliative care are scarce. OBJECTIVES: The goals of this pre-post study, conducted among patients with advanced cancer receiving outpatient palliative care, were to evaluate the feasibility and acceptability of chaplain-delivered spiritual care, utilizing the Spiritual Assessment and Intervention Model ("Spiritual AIM"); and to gather pilot data on Spiritual AIM's effects on spiritual well-being, religious and cancer-specific coping, and physical and psychological symptoms...
July 20, 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28736039/cloning-identification-and-characterization-of-a-novel-%C3%AE%C2%BA-carrageenase-from-marine-bacterium-cellulophaga-lytica-strain-n5-2
#15
Hongli Cui, Yuxin Peng, Bowen Zhao, Yuqing Liu, Fengjia Chen, Haige Wu, Ziang Yao
A novel κ-carrageenase gene (Cly-κ-car) was cloned and heterologously expressed from marine bacterium Cellulophaga lytica strain N5-2. The gene comprised an open reading frame of 1,488bp encoding 495 amino acid residues. The deduced protein had a calculated molecular weight of 55.24kDa with an estimated isoelectric point of 9.90. Multiple alignment analysis revealed that Cly-κ-CAR shared identity with κ-carrageenases from Zobellia sp. M-2 (46%), Zobellia galactanivorans (42%) and Rhodopirellula islandica (38%)...
July 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28735961/binding-of-pmclipsp2-to-microbial-cell-wall-components-and-activation-of-the-propo-activating-system-in-the-black-tiger-shrimp-penaeus-monodon
#16
Narach Khoratanakulchai, Piti Amparyup, Anchalee Tassanakajon
Clip domain serine proteinases (ClipSPs) play an important role in the prophenoloxidase-activating (proPO) system. In the shrimp Penaeus monodon, the ClipSP PmClipSP2 has been previously shown to bind to microbial polysaccharides (LPS and β-1,3-glucan) and likely activates the proPO system. To reveal the binding site of the PmClipSP2 protein, the N-terminal clip domain (Clip-PmClipSP) and C-terminal SP domain (SP-PmClipSP2) were separately cloned. The recombinant proteins were then assayed for their binding properties and involvement in proPO activation...
July 20, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28735320/in-vitro-interaction-between-fumonisin-b-sub-1-sub-and-the-intestinal-microflora-of-pigs
#17
Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, Istvan Bors, Andras Bonai, Brigitta Bota, Judit Szabo Fodor
The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735309/safety-evaluation-of-enterocin-producer-enterococcus-sp-strains-isolated-from-traditional-turkish-cheeses
#18
Mine Avcı, Banu Özden Tuncer
The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates. Multiple enterocin structural genes were found in 7 strains...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735307/influence-of-pseudomonas-and-bacillus-strains-isolated-from-lolium-perenne-rhizospheric-soil-in-vojvodina-serbia-on-planth-growth-and-soil-microbial-communities
#19
Stamenov R Dragana, Simonida Đurić, Timea I Hajnal Jafari, Snezana Anđelković
The aim of this study was the isolation of Pseudomonas sp. and Bacillus sp. strains from rhizospheric soil and monitoring the impact of two isolates denoted as P12 (Pseudomonas sp.) and B1 (Bacillus sp.) on the parameters of English ryegrass (Lolium perenne) yield and activity of the soil microbial communities. During 2012-2014, a plot experiment was set up following the randomized block system. Better effect on the plant growth was recorded with the use of Pseudomonas sp. P12 isolate than with Bacillus sp...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735243/interactions-between-pteris-vittata-l-genotypes-and-a-polycyclic-aromatic-hydrocarbon-pah-degrading-bacterium-alcaligenes-sp-in-arsenic-uptake-and-pah-dissipation
#20
Lu Sun, Ganghui Zhu, Xiaoyong Liao, Xiulan Yan
The effects of two Pteris vittata L. accessions and a polycyclic aromatic hydrocarbon (PAH)-degrading bacterium (Alcaligenes sp.) on arsenic (As) uptake and phenanthrene dissipation were studied. The Alcaligenes sp. survived in the rhizosphere and improved soil As bioavailability with co-exposure. However, bacterial inoculation altered Pteris vittata L. stress tolerance, and substantially affected the As distribution in the rhizosphere of the two P. vittata accessions. Bacterial inoculation was beneficial to protect the Guangxi accession against the toxic effects, and significantly increased plant As and phenanthrene removal ratios by 27...
July 20, 2017: Environmental Pollution
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