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https://www.readbyqxmd.com/read/28922839/first-description-of-a-new-uncultured-epsilon-sulfur-bacterium-colonizing-marine-mangrove-sediment-in-the-caribbean-thiovulum-sp-strain-karukerense
#1
Olivier Gros
Here, the first description is reported of an epsilon sulfur-oxidizing bacterium from sulfide-rich sediments of marine mangrove in the Caribbean. By transition electron microscopy it was shown that this new strain contains intracytoplasmic large internal sulfur granules, which was confirmed by energy-dispersive X-ray spectroscopy analyses performed using an environmental scanning electron microscope. The sulfur distribution obtained for this sulfur-oxidizing bacterial strain allowed us to conclude that elemental sulfur is formed as an intermediate oxidation product and stored intracellularly...
October 2, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28922597/core-shell-silver-nanoparticles-in-endodontic-disinfection-solutions-enable-long-term-antimicrobial-effect-on-oral-biofilms
#2
Elif Ertem Bekdemir, Beatrice Gutt, Flavia Zuber, Sergio Allegri, Benjamin Le Ouay, Selma Mefti, Kitty Formentin, Francesco Stellacci, Qun Ren
To achieve effective long-term disinfection of the root canals, we synthesized core-shell silver nanoparticles (AgNPs@SiO2) and used them to develop two irrigation solutions containing sodium phytate (SP) and ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), respectively. Ex vivo studies with instrumented root canals revealed that the developed irrigation solutions can effectively remove the smear layer from the dentinal surfaces. Further in vitro experiments with single and multi-species biofilms demonstrated for the first time that AgNPs@SiO2 based irrigation solutions possess excellent antimicrobial activities for at least 7 days, whereas the bare AgNPs lose the activity almost immediately and do not show any antibacterial activity after 2 days...
September 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28922521/molecular-and-morphological-characterization-of-anncaliia-azovica-sp-n-microsporidia-infecting-niphargogammarus-intermedius-crustacea-amphipoda-from-the-azov-sea
#3
Yuri S Tokarev, Yuliya Y Sokolova, Aleksandra A Vasilieva, Irma V Issi
Five out of one hundred adults of Niphargogammarus intermedius caught at the Azov sea shore were found to be infected with microsporidia. The infection was found in the subcuticular fat body and myocytes. Parasites developed in direct contact with host cells, displayed a disporoblastic sporogony and diplokaryotic arrangement of nuclei at all stages. Spores were oval, 4.6-5.8x2.6-3.0 μm. Exospore appendages, vesicular-tubular secretions and the anisofilar polar filament indicated a similarity to Anncaliia species...
September 18, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28922500/total-synthesis-of-the-diglycosidic-tetramic-acid-ancorinoside-a
#4
Markus Petermichl, Rainer Schobert
Ancorinoside A, a metabolite of a sponge Ancorina sp., was prepared in 18 steps as the first derivative of this class of glycosylated 3-acyltetramic acids. It features a β-D-glucopyranosyl-(1→4)-β-D-galacturonic acid linked to a D-aspartic acid derived tetramic acid via a 3-docosanoyl spacer. The diglycoside was built up by connecting the protected monosaccharides D-galactose and D-glucose via a thioglycoside glycosylation. Attachment of the spacer by a subsequent Schmidt glycosylation of this diglycoside, TEMPO oxidation to the uronic acid, functionalisation of the spacer terminus with an N-(β-ketoacyl)aspartate, and a final Dieckmann cyclisation of the latter were the key steps leading to ancorinoside A...
September 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28922498/c-sp%C3%A2-h-bond-activation-by-vinylidene-gold-i-complexes-a-concerted-asynchronous-or-stepwise-process
#5
Johannes E M N Klein, Gerald Knizia, Laura Nunes Dos Santos Comprido, Johannes Kästner, A Stephen K Hashmi
A detailed analysis of the C(sp³)-H activation process by vinylidene Au(I) complexes is described based on an intrinsic bond orbital analysis. Based on our analysis this event can be divided into three phases: (i) hydride transfer, (ii) C-C bond formation, and (iii) σ to π rearrangement of the lone pair coordinated to Au. Small perturbations of the system lead to either a concerted asynchronous reaction, or a stepwise reaction featuring an intermediate with a C-H-C three-centre two-electron (3c-2e) bond...
September 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28922412/walker-occupancy-has-an-impact-on-changing-airborne-bacterial-communities-in-an-underground-pedestrian-space-as-small-dust-particles-increased-with-raising-both-temperature-and-humidity
#6
Torahiko Okubo, Takako Osaki, Eriko Nozaki, Akira Uemura, Kouhei Sakai, Mizue Matushita, Junji Matsuo, Shinji Nakamura, Shigeru Kamiya, Hiroyuki Yamaguchi
Although human occupancy is a source of airborne bacteria, the role of walkers on bacterial communities in built environments is poorly understood. Therefore, we visualized the impact of walker occupancy combined with other factors (temperature, humidity, atmospheric pressure, dust particles) on airborne bacterial features in the Sapporo underground pedestrian space in Sapporo, Japan. Air samples (n = 18; 4,800L/each sample) were collected at 8:00 h to 20:00 h on 3 days (regular sampling) and at early morning / late night (5:50 h to 7:50 h / 22:15 h to 24:45 h) on a day (baseline sampling), and the number of CFUs (colony forming units) OTUs (operational taxonomic units) and other factors were determined...
2017: PloS One
https://www.readbyqxmd.com/read/28922100/frequency-and-antibiotic-resistance-of-bacteria-implicated-in-community-urinary-tract-infections-in-north-aveiro-between-2011-and-2014
#7
Tânia Costa, Inês Linhares, Ricardo Ferreira, Jasmin Neves, Adelaide Almeida
AIMS: The present study aims to evaluate the predominance of uropathogens responsible for urinary tract infection (UTI) and determine their resistance patterns, to assess if the recommended empirical treatment is appropriate for the studied population. Samples were collected in Aveiro (Portugal) from an ambulatory service between June 2011 and June 2014. RESULTS: From the 4,270 positive urine samples for UTI, 3,561 (83%) were from women and only 709 (17%) were from men...
September 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28922022/implementing-a-collaborative-coaching-intervention-for-professionals-providing-care-to-children-and-their-families-an-exploratory-study
#8
Sandy K Tatla, Dori Howard, Alda Antunes Silvestre, Stacey Burnes, Meghan Husson, Tal Jarus
The growing complexity of healthcare requires family and interprofessional partnerships to deliver effective care. Interprofessional coaching can enhance family-centred practice and collaboration. The purpose of this study was to explore the acceptability and feasibility of collaborative coaching training to improve family centredness within acute paediatric rehabilitation. Using a participatory action design, service providers (SPs; n = 36) underwent a 6-month coaching programme involving coaching workshops, learning triads, and tailored sessions with a licensed coach...
September 18, 2017: Journal of Interprofessional Care
https://www.readbyqxmd.com/read/28921982/mortiamides-a-d-cyclic-heptapeptides-from-a-novel-mortierella-sp-obtained-from-frobisher-bay
#9
Alyssa L Grunwald, Fabrice Berrue, Andrew W Robertson, David P Overy, Russell G Kerr
Four new cyclic heptapeptides, mortiamides A-D (1-4), were obtained from a novel Mortierella sp. isolate obtained from marine sediment collected from the intertidal zone of Frobisher Bay, Nunavut, Canada. The structures of the compounds were elucidated by NMR spectroscopy and tandem mass spectrometry. The absolute configurations of the amino acids were determined using Marfey's method. Localization of l and d amino acids within each compound was ascertained by retention time comparison of the partial hydrosylate products of each compound to synthesized dipeptide standards using LC-HRMS...
September 18, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28921896/evaluating-potential-risks-of-food-allergy-and-toxicity-of-soy-leghemoglobin-expressed-in-pichia-pastoris
#10
Yuan Jin, Xiaoyun He, Kwame Andoh-Kumi, Rachel Z Fraser, Mei Lu, Richard E Goodman
SCOPE: The Soybean (Glycine max) leghemoglobin c2 (LegHb) gene was introduced into Pichia pastoris yeast for sustainable production of a heme-carrying protein, for organoleptic use in plant-based meat. The potential allergenicity and toxicity of LegHb and 17 Pichia host-proteins each representing ≥1% of total protein in production batches were evaluated by literature review, bioinformatics sequence comparisons to known allergens or toxins, and in vitro pepsin digestion. METHODS AND RESULTS: Literature searches found no evidence of allergenicity or toxicity for these proteins...
September 18, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28921766/effects-of-zoochory-on-the-spatial-genetic-structure-of-plant-populations
#11
Tiziana A Gelmi-Candusso, Eckhard W Heymann, Katrin Heer
Spatial genetic structure (SGS) of plants results from the non-random distribution of related individuals. SGS provides information on gene flow and spatial patterns of genetic diversity within populations. Seed dispersal creates the spatial template for plant distribution. Thus, in zoochorous plants, dispersal mode and disperser behavior might have a strong impact on SGS. However, many studies only report the taxonomic group of seed dispersers, without further details. The recent increase in studies on SGS provides the opportunity to review findings and test for the influence of dispersal mode, taxonomic affiliation of dispersers and their behavior...
September 16, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28921438/purification-methods-for-recombinant-factor-viii-expressed-in-human-liver-sk-hep-cells
#12
Vladimir Granovski, Mario Soares Abreu-Neto, Dimas Tadeu Covas
Coagulation factor VIII is one of the largest proteins attempted to be expressed in recombinant form. A very complex and labile protein which has a very short half-live and need a fast and efficient purification chain. Here, we describe a simple purification sequence using multimodal Capto MMC, affinity FVIII select and ion exchange SP-Fastflow chromatography steps without subjecting the target molecule to mechanical and temperature stress, separating impurities from rFVIII using net charge, hydrophobicity, and affinity of the molecules...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28920853/flavobacterium-chuncheonense-sp-nov-and-flavobacterium-luteum-sp-nov-isolated-from-a-freshwater-lake
#13
Miri Park, Gi Gyun Nam, Suhyun Kim, Hyoung Tae Jeon, Yochan Joung, Jang-Cheon Cho
Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013T and IMCC26026T, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013T and IMCC26026T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013T and IMCC26026T were 37...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920851/loktanella-acticola-sp-nov-isolated-from-seawater
#14
Sooyeon Park, Su Jung Choi, Sung-Min Won, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated OISW-6T, was isolated from seawater near Oido, a South Korean island, and subjected to a polyphasic taxonomic study. Strain OISW-6T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-6T fell within the clade comprising the type strains of Loktanella species. Strain OISW-6T exhibited 16S rRNA gene sequence similarity values of 97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920850/ramlibacter-alkalitolerans-sp-nov-alkali-tolerant-bacterium-isolated-from-soil-of-ginseng
#15
Do-Hoon Lee, Chang-Jun Cha
A novel bacterial strain, designated CJ661T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661T showed that it belongs to the genus Ramlibacter within the family Comamonadaceae and was most closely related to Ramlibacter ginsenosidimutans KCTC 22276T (98.1 %), followed by Ramlibacter henchirensis DSM 14656T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920849/flavimarina-flava-sp-nov-isolated-from-salicornia-herbacea
#16
Eui-Sang Cho, In-Tae Cha, Jung-Min Park, Hak-Jong Choi, Jong Hun Lee, Seong Woon Roh, Eun-Ah Cho, Mee-Hyang Kweon, Young-Do Nam, Myung-Ji Seo
A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091T. It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73T with 94.8 % similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920848/dyella-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#17
Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel strain, DKC-1T, was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1T was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20-37 °C, at pH 4.5-9.0 and with 0-3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1T formed a clade within the members of the genus Dyella and showed highest sequence similarities to Dyella japonica XD53T (98...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920847/spirosoma-carri-sp-nov-isolated-from-an-automobile-air-conditioning-system
#18
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, So Yoon Park, Jong-Ok Ka
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0406T, was isolated from an automobile evaporator core collected in Korea. The cells were non-motile, aerobic and rod-shaped. The strain grew at 15-37 °C (optimum, 25 °C), at pH 6.0-7.0 (optimum, 6.5) and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetically, the strain was related to members of the genus Spirosoma(93.7-90.7 % 16S rRNA sequence similarities) and showed the highest sequence similarity of 93.7 % to Spirosomapulveris JSH5-14T...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920842/marivibrio-halodurans-gen-nov-sp-nov-a-marine-bacterium-in-the-family-rhodospirillaceae-isolated-from-underground-rock-salt
#19
Shaoxing Chen, Yao Xu, Chen Zheng, Li-Xia Ke
Gram-negative, spiral or curved rod-shaped cells of a bacterial strain, designated ZC80T, were isolated from a rock salt sample collected at Yunnan salt mine, China. Analysis of the strain's 16S rRNA gene sequence revealed a clear affiliation of this novel strain within the family Rhodospirillaceae. Strain ZC80T formed a robust cluster with Pelagibius litoralis CL-UU02T at a 16S rRNA gene sequence similarity level of 88.1 %. Strain ZC80T shared no more than 91.0 % 16S rRNA gene sequence similarity with the type strains of other species in the family Rhodospirillaceae...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920841/desertibacillus-haloalkaliphilus-gen-nov-sp-nov-isolated-from-a-saline-desert
#20
Hitarth B Bhatt, M Azmatunnisa Begum, Sasikala Chintalapati, Venkata Ramana Chintalapati, Satya P Singh
Two Gram-stain-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains KJ1-10-99T and KJ1-10-93, were isolated from a saline desert of Little Rann of Kutch, Gujarat, India. Analysis of 16S rRNA gene sequences revealed that the isolates belonged to the family Bacillaceae and were closely related to each other with 16S rRNA gene sequence similarity of 99.9 %. However, these two isolates formed a novel phylogenetic branch within this family. Both strains were aerobic, catalase and oxidase positive, and could grow optimally at 37 °C and pH 9...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
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