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https://www.readbyqxmd.com/read/29786904/visible-light-induced-supra-amphiphilic-switch-leads-to-transition-from-supramolecular-nanosphere-to-nanovesicle-activated-by-pillar-5-arene-based-host-guest-interaction
#1
Pengyu Li, Qianfang Yao, Baozhong Lü, Guiping Ma, Meizhen Yin
A photoresponsive host-guest supramolecular complex (WP5⊃G) constructed by water-soluble pillar[5]arene (WP5) and spiropyran derivative (G) is presented. The spontaneous isomerization of G from spiropyran (SP) form to ring-opened merocyanine (MC) form happens either alone or in WP5⊃G in aqueous media. Irradiated by visible light, G can be converted into SP form completely and the hydrophilicity will be changed. G and WP5⊃G are both verified to self-assemble into nanospheres. Upon exposure to visible light, WP5⊃G reassemble into nanovesicles due to the change of supra-amphiphilicity, while G alone does not have this transition...
May 22, 2018: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/29786766/bacterial-dna-detected-on-pathologically-changed-heart-valves-using-16s-rrna-gene-amplification
#2
Miroslava Chalupova, Anna Skalova, Tomas Hajek, Lenka Geigerova, Dana Kralova, Pavel Liska, Hana Hecova, Jiri Molacek, Jaroslav Hrabak
Nowadays, dental diseases are one of the most common illnesses in the world. Some of them can lead to translocation of oral bacteria to the bloodstream causing intermittent bacteraemia. Therefore, a potential association between oral infection and cardiovascular diseases has been discussed in recent years as a result of adhesion of oral microbes to the heart valves. The aim of this study was to detect oral bacteria on pathologically changed heart valves not caused by infective endocarditis. In the study, patients with pathologically changed heart valves were involved...
May 22, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29786696/crystal-structure-of-dihydrodipicolinate-reductase-padhdpr-from-paenisporosarcina-sp-tg-14-structural-basis-for-nadph-preference-as-a-cofactor
#3
Chang Woo Lee, Sun-Ha Park, Sung Gu Lee, Hyun Ho Park, Hak Jun Kim, HaJeung Park, Hyun Park, Jun Hyuck Lee
Dihydrodipicolinate reductase (DHDPR) is a key enzyme in the diaminopimelate- and lysine-synthesis pathways that reduces DHDP to tetrahydrodipicolinate. Although DHDPR uses both NADPH and NADH as a cofactor, the structural basis for cofactor specificity and preference remains unclear. Here, we report that Paenisporosarcina sp. TG-14 PaDHDPR has a strong preference for NADPH over NADH, as determined by isothermal titration calorimetry and enzymatic activity assays. We determined the crystal structures of PaDHDPR alone, with its competitive inhibitor (dipicolinate), and the ternary complex of the enzyme with dipicolinate and NADPH, with results showing that only the ternary complex had a fully closed conformation and suggesting that binding of both substrate and nucleotide cofactor is required for enzymatic activity...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29786655/bioactive-pyridone-alkaloids-from-a-deep-sea-derived-fungus-arthrinium-sp-ujnmf0008
#4
Jie Bao, Huijuan Zhai, Kongkai Zhu, Jin-Hai Yu, Yuying Zhang, Yinyin Wang, Cheng-Shi Jiang, Xiaoyong Zhang, Yun Zhang, Hua Zhang
Eight new 4-hydroxy-2-pyridone alkaloids arthpyrones D⁻K ( 1 ⁻ 8 ), along with two known analogues apiosporamide ( 9 ) and arthpyrone B ( 10 ), were isolated from a deep-sea-derived fungus Arthrinium sp. UJNMF0008. The structures of the isolated compounds were elucidated on the basis of spectroscopic methods with that of 1 being established by chemical transformation and X-ray diffraction analysis. Compounds 1 and 2 bore an ester functionality linking the pyridone and decalin moieties first reported in this class of metabolites, while 3 and 4 incorporated a rare natural hexa- or tetrahydrobenzofuro[3,2- c ]pyridin-3(2 H )-one motif...
May 22, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29786505/can-pallars-i-llobateres-a-new-hominoid-bearing-locality-from-the-late-miocene-of-the-vall%C3%A3-s-pened%C3%A3-s-basin-ne-iberian-peninsula
#5
David M Alba, Isaac Casanovas-Vilar, Marc Furió, Israel García-Paredes, Chiara Angelone, Sílvia Jovells-Vaqué, Àngel H Luján, Sergio Almécija, Salvador Moyà-Solà
In the Iberian Peninsula, Miocene apes (Hominoidea) are generally rare and mostly restricted to the Vallès-Penedès Basin. Here we report a new hominoid maxillary fragment with M2 from this basin. It was surface-collected in March 2017 from the site of Can Pallars i Llobateres (CPL, Sant Quirze del Vallès), where fossil apes had not been previously recorded. The locality of provenance (CPL-M), which has delivered no further fossil remains, is located very close (ca. 50 m) to previously known CPL outcrops, and not very far (ca...
May 18, 2018: Journal of Human Evolution
https://www.readbyqxmd.com/read/29786499/scheffersomyces-stambukii-f-a-sp-nov-a-d-xylose-fermenting-species-isolated-from-rotting-wood
#6
Mariana R Lopes, Thiago M Batista, Glória R Franco, Lucas R Ribeiro, Ana R O Santos, Carolina Furtado, Rennan G Moreira, Aristóteles Goes-Neto, Marcos J S Vital, Luiz H Rosa, Marc-André Lachance, Carlos A Rosa
Two isolates representing a new species of Scheffersomyces were isolated from rotting wood samples collected in an Amazonian forest ecosystem in Brazil. Analysis of the sequences of the D1/D2 domains showed that this new species is phylogenetically related to Scheffersomyces NYMU 15730, a species without a formal description, and the two are in an early emerging position with respect to the xylose-fermenting subclade containing Scheffersomyces titanus and Scheffersomyces stipitis. Phylogenomic analyses using 474 orthologous genes placed the new species in an intermediary position between Scheffersomyces species and the larger genus Spathaspora and the Candida albicans/Lodderomyces clade...
May 22, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29786497/massilia-armeniaca-sp-nov-isolated-from-desert-soil
#7
Mengnan Ren, Xiaoyan Li, Yueqi Zhang, Yu Jin, Siqi Li, Haidong Huang
A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, strain ZMN-3T , was isolated from desert soil sample collected from Ongniod Qi, Inner Mongolia, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZMN-3T was affiliated with the genus Massilia and showed the highest similarity to Massilia humi THG S6-8T (98.9 %) and Massilia buxea A9T (98.2 %). In partial gyrB and lepA sequences, the highest similarity of strain ZMN-3T and M. humi THG S6-8T were 95.9 and 96.8 %, respectively...
May 22, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29786161/evidence-for-horizontal-gene-transfer-and-separation-of-effector-recognition-from-effector-function-revealed-by-analysis-of-effector-genes-shared-between-cape-gooseberry-and-tomato-infecting-formae-speciales-of-fusarium-oxysporum
#8
Jaime Simbaqueba, Ann-Maree Catanzariti, Carolina González, David A Jones
RNAseq reads from cape-gooseberry plants (Physalis peruviana) infected with Fusarium oxysporum f. sp. physali (Foph) were mapped against the lineage-specific transcriptome of Fusarium oxysporum f. sp. lycopersici (Fol) to look for putative effector genes. Homologues of Fol SIX1 (designated SIX1a and SIX1b), SIX7, SIX10, SIX12, SIX15 and Ave1 were identified. The near identity of the Foph and Fol SIX7, SIX10 and SIX12 genes and their intergenic regions suggest that this gene cluster may have undergone recent lateral transfer...
May 22, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29786025/resident-perspectives-on-communication-training-that-utilizes-immersive-virtual-reality
#9
Francis J Real, Dominick DeBlasio, Nicholas J Ollberding, David Davis, Bradley Cruse, Daniel Mclinden, Melissa D Klein
Background: Communication skills can be difficult to teach and assess in busy outpatient settings. These skills are important for effective counseling such as in cases of influenza vaccine hesitancy. It is critical to consider novel educational methods to supplement current strategies aimed at teaching relational skills. Methods: An immersive virtual reality (VR) curriculum on addressing influenza vaccine hesitancy was developed using Kern's six-step approach to curriculum design...
September 2017: Education for Health: Change in Training & Practice
https://www.readbyqxmd.com/read/29785671/agarolytic-culturable-bacteria-associated-with-three-antarctic-subtidal-macroalgae
#10
Verónica Sánchez Hinojosa, Joel Asenjo, Sergio Leiva
Bacterial communities of Antarctic marine macroalgae remain largely underexplored in terms of diversity and biotechnological applications. In this study, three Antarctic subtidal macroalgae (Himantothallus grandifolius, Pantoneura plocamioides and Plocamium cartilagineum), two of them endemic of Antarctica, were investigated as a source for isolation of agar-degrading bacteria. A total of 21 epiphytic isolates showed agarolytic activity at low temperature on agar plates containing agar as the sole carbon source...
May 21, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29785634/a-cluster-of-five-genes-essential-for-the-utilization-of-dihydroxamate-xenosiderophores-in-synechocystis-sp-pcc-6803
#11
Tobias A Obando S, Michael M Babykin, Vladislav V Zinchenko
The unicellular freshwater cyanobacterium Synechocystis sp. PCC 6803 is capable of using dihydroxamate xenosiderophores, either ferric schizokinen (FeSK) or a siderophore of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 (SAV), as the sole source of iron in the TonB-dependent manner. The fecCDEB1-schT gene cluster encoding a siderophore transport system that is involved in the utilization of FeSK and SAV in Synechocystis sp. PCC 6803 was identified. The gene schT encodes TonB-dependent outer membrane transporter, whereas the remaining four genes encode the ABC-type transporter FecB1CDE formed by the periplasmic binding protein FecB1, the transmembrane permease proteins FecC and FecD, and the ATPase FecE...
May 21, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29785605/selection-of-inactivation-medium-for-fungal-spores-in-clinical-wastes-by-supercritical-carbon-dioxide
#12
Efaq Noman, Nik Norulaini Nik Ab Rahman, Adel Al-Gheethi, Hideyuki Nagao, Balkis A Talip, Omar Ab Kadir
The present study aimed to select the best medium for inactivation of Aspergillus fumigatus, Aspergillus spp. in section Nigri, A. niger, A. terreus var. terreus, A. tubingensis, Penicillium waksmanii, P. simplicissimum, and Aspergillus sp. strain no. 145 spores in clinical wastes by using supercritical carbon dioxide (SC-CO2 ). There were three types of solutions used including normal saline, seawater, distilled water, and physiological saline with 1% of methanol; each solution was tested at 5, 10, and 20 mL of the water contents...
May 21, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29785529/isolation-and-characterization-of-bacillus-sp-gfp-2-a-novel-bacillus-strain-with-antimicrobial-activities-from-whitespotted-bamboo-shark-intestine
#13
Jia Wu, Guoqiang Xu, Yangyang Jin, Cong Sun, Li Zhou, Guodong Lin, Rong Xu, Ling Wei, Hui Fei, Dan Wang, Jianqing Chen, Zhengbing Lv, Kuancheng Liu
The abuse of antibiotics and following rapidly increasing of antibiotic-resistant pathogens is the serious threat to our society. Natural products from microorganism are regarded as the important substitution antimicrobial agents of antibiotics. We isolated a new strain, Bacillus sp. GFP-2, from the Chiloscyllium plagiosum (Whitespotted bamboo shark) intestine, which showed great inhibitory effects on the growth of both Gram-positive and Gram-negative bacteria. Additionally, the growth of salmon was effectively promoted when fed with inactivated strain GFP-2 as the inhibition agent of pathogenic bacteria...
May 22, 2018: AMB Express
https://www.readbyqxmd.com/read/29785409/-de-novo-genome-and-transcriptome-resources-of-the-adzuki-bean-borer-ostrinia-scapulalis-lepidoptera-crambidae
#14
B Gschloessl, F Dorkeld, P Audiot, A Bretaudeau, C Kerdelhué, R Streiff
We present a draft genome assembly with a de novo prediction and automated functional annotation of coding genes, and a reference transcriptome of the Adzuki bean borer, Ostrinia scapulalis , based on RNA sequencing of various tissues and developmental stages. The genome assembly spans 419 Mb, has a GC content of 37.4% and includes 26,120 predicted coding genes. The reference transcriptome holds 33,080 unigenes and contains a high proportion of a set of genes conserved in eukaryotes and arthropods, used as quality assessment of the reconstructed transcripts...
April 2018: Data in Brief
https://www.readbyqxmd.com/read/29785057/computational-redesign-of-enzymes-for-regio-and-enantioselective-hydroamination
#15
Ruifeng Li, Hein J Wijma, Lu Song, Yinglu Cui, Marleen Otzen, Yu'e Tian, Jiawei Du, Tao Li, Dingding Niu, Yanchun Chen, Jing Feng, Jian Han, Hao Chen, Yong Tao, Dick B Janssen, Bian Wu
Introduction of innovative biocatalytic processes offers great promise for applications in green chemistry. However, owing to limited catalytic performance, the enzymes harvested from nature's biodiversity often need to be improved for their desired functions by time-consuming iterative rounds of laboratory evolution. Here we describe the use of structure-based computational enzyme design to convert Bacillus sp. YM55-1 aspartase, an enzyme with a very narrow substrate scope, to a set of complementary hydroamination biocatalysts...
May 21, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29785041/dark-septate-endophytes-isolated-from-a-xerophyte-plant-promote-the-growth-of-ammopiptanthus-mongolicus-under-drought-condition
#16
Xia Li, Xueli He, Lifeng Hou, Ying Ren, Shaojie Wang, Fang Su
Dark septate endophytes (DSE) may facilitate plant growth and stress tolerance in stressful ecosystems. However, little is known about the response of plants to non-host DSE fungi isolated from other plants, especially under drought condition. This study aimed to seek and apply non-host DSE to evaluate their growth promoting effects in a desert species, Ammopiptanthus mongolicus, under drought condition. Nine DSE strains isolated from a super-xerophytic shrub, Gymnocarpos przewalskii, were identified and used as the non-host DSE...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784882/phenotypic-assessment-suggests-multiple-start-codons-for-hetn-an-inhibitor-of-heterocyst-differentiation-in-anabaena-sp-strain-pcc-7120
#17
Orion S Rivers, Silvia Beurmann, Allexa Dow, Loralyn M Cozy, Patrick Videau
Multicellular organisms must carefully regulate the timing, number, and location of specialized cellular development. In the filamentous cyanobacterium Anabaena sp. strain PCC 7120, nitrogen-fixing heterocysts are interspersed between vegetative cells in a periodic pattern to achieve optimal exchange of bioavailable nitrogen and reduced carbon. The spacing between heterocysts is regulated by the activity of two developmental inhibitors: PatS and HetN. PatS functions to create a de novo pattern from a homogenous field of undifferentiated cells, while HetN maintains the pattern throughout subsequent growth...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784799/amorphous-boron-oxide-at-megabar-pressures-via-inelastic-x-ray-scattering
#18
Sung Keun Lee, Yong-Hyun Kim, Paul Chow, Yunming Xiao, Cheng Ji, Guoyin Shen
Structural transition in amorphous oxides, including glasses, under extreme compression above megabar pressures (>1 million atmospheric pressure, 100 GPa) results in unique densification paths that differ from those in crystals. Experimentally verifying the atomistic origins of such densifications beyond 100 GPa remains unknown. Progress in inelastic X-ray scattering (IXS) provided insights into the pressure-induced bonding changes in oxide glasses; however, IXS has a signal intensity several orders of magnitude smaller than that of elastic X-rays, posing challenges for probing glass structures above 100 GPa near the Earth's core-mantle boundary...
May 21, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29784440/corrigendum-to-bifidobacterium-aerophilum-sp-nov-bifidobacterium-avesanii-sp-nov-and-bifidobacterium-ramosum-sp-nov-three-novel-taxa-from-the-faeces-of-cotton-top-tamarin-saguinus-oedipus-l-syst-appl-microbiol-39-2016-229-236
#19
Samanta Michelini, Monica Modesto, Gianfranco Filippini, Caterina Spiezio, Camillo Sandri, Bruno Biavati, Annamaria Pisi, Paola Mattarelli
No abstract text is available yet for this article.
May 18, 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29783627/characteristics-of-the-copper-zinc-superoxide-dismutase-of-a-hadal-sea-cucumber-paelopatides-sp-from-the-mariana-trench
#20
Yanan Li, Xue Kong, Jiawei Chen, Helu Liu, Haibin Zhang
Superoxide dismutases (SODs) are among the most important antioxidant enzymes and show great potential in preventing adverse effects during therapeutic trials. In the present study, cloning, expression, and characterization of a novel Cu,Zn superoxide dismutase (Ps-Cu,Zn-SOD) from a hadal sea cucumber ( Paelopatides sp.) were reported. Phylogenetic analysis showed that Ps-Cu,Zn-SOD belonged to a class of intracellular SOD. Its K m and V max were 0.0258 ± 0.0048 mM and 925.1816 ± 28.0430 units/mg, respectively...
May 18, 2018: Marine Drugs
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