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https://www.readbyqxmd.com/read/29792900/the-sulfoconjugation-of-androstenone-and-dehydroepiandrosterone-by-human-and-porcine-sulfotransferase-enzymes
#1
Heidi Laderoute, Christine Bone, E James Squires
Porcine sulfotransferase 2A1 (pSULT2A1) is a key enzyme involved in the testicular and hepatic sulfoconjugation of steroids such as dehydroepiandrosterone (DHEA) and potentially androstenone. This latter steroid is a major cause of boar taint, which is an unpleasant off-odour and off-flavour in pork from male pigs. Sulfotransferase 2B1 (pSULT2B1) may also be important, although no direct evidence exists for its involvement in sulfoconjugation of steroids. The purpose of this study was to investigate the sulfoconjugation activity of human and porcine sulfotransferases towards DHEA and androstenone...
May 21, 2018: Steroids
https://www.readbyqxmd.com/read/29792369/gene-delivery-in-the-cornea-in-vitro-ex-vivo-evaluation-of-solid-lipid-nanoparticle-based-vectors
#2
Mónica Vicente-Pascual, Andrea Albano, María Á Solinís, Loredana Serpe, Alicia Rodríguez-Gascón, Federica Foglietta, Elisabetta Muntoni, Josune Torrecilla, Ana Del Pozo-Rodríguez, Luigi Battaglia
AIM: Inflammation is a process that underlies sight-threatening ocular surface diseases, and gene supplementation with the plasmid that encodes for p-IL10 will allow the sustained de novo synthesis of the cytokine to occur in corneal cells, and provide a long-term anti-inflammatory effect. This work describes the development of solid lipid nanoparticle systems for the delivery of p-IL10 to transfect the cornea. RESULTS: In vitro, vectors showed suitable features as nonviral vectors (size, ζ-potential, DNA binding, protection and release), and they were able to enter and transfect human corneal epithelial cells...
May 24, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29791759/ctnnal1-inhibits-ozone-induced-epithelial-mesenchymal-transition-in-human-bronchial-epithelial-cells
#3
Meiling Tan, Caixia Liu, Wenjie Huang, Linhong Deng, Xiaoqun Qin, Yang Xiang
NEW FINDINGS: What is the central question of this study? In our previous work, CTNNAL1 (catenin alpha-like 1), an asthma-related epithelial adhesion molecule, was found to play a vital role in airway epithelial wound repair. The effect of CTNNAL1 on airway EMT has not been previously explored. What is the main finding and its importance? CTNNAL1 inhibits ozone-induced airway EMT features and mediated by repressing the expression of Twist1 mRNA and reducing TGF-β1 levels. These findings contribute to our understanding of the pathology of airway EMT and may indicate a possible therapeutic target for airway remodelling in bronchial asthma...
May 23, 2018: Experimental Physiology
https://www.readbyqxmd.com/read/29791590/lncrna-ccat1-promotes-cell-proliferation-migration-and-invasion-by-down-regulation-of-mir-143-in-ftc-133-thyroid-carcinoma-cell-line
#4
Tianzheng Yang, Hongyan Zhai, Ruihong Yan, Zhenhu Zhou, Lei Gao, Luqing Wang
Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. FTC-133 cells were transfected with CCAT1 expressing vector, CCAT1 shRNA, miR-143 mimic, and miR-143 inhibitor, respectively. After different treatments, cell viability, proliferation, migration, invasion, and apoptosis were measured...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29791586/hypoxia-induced-hyperpermeability-of-rat-glomerular-endothelial-cells-involves-hif-2%C3%AE-mediated-changes-in-the-expression-of-occludin-and-zo-1
#5
Peng-Li Luo, Yan-Jun Wang, Yan-Yan Yang, Jia-Jia Yang
This study aimed to investigate the role of hypoxia-inducible factor-2α (HIF-2α) in the expression of tight junction proteins and permeability alterations in rat glomerular endothelial cells (rGENCs) under hypoxia conditions. The expression level of HIF-2α and tight junction proteins (occludin and ZO-1) in rGENCs were examined following 5% oxygen density exposure at different treatment times. HIF-2α lentivirus transfection was used to knockdown HIF-2α expression. Cells were divided into four groups: 1) control group (rGENCs were cultured under normal oxygen conditions), 2) hypoxia group (rGENCs were cultured under hypoxic conditions), 3) negative control group (rGENCs were infected with HIF-2α lentivirus negative control vectors and cultured under hypoxic conditions), and 4) Len group (rGENCs were transfected with HIF-2α lentivirus and cultured under hypoxic conditions)...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29790281/suppressing-tawny-crazy-ant-nylanderia-fulva-by-rnai-technology
#6
Jia Meng, Jiaxin Lei, Andrew Davitt, Jocelyn R Holt, Jian Huang, Roger Gold, Edward L Vargo, Aaron M Tarone, Keyan Zhu-Salzman
The tawny crazy ant (Nylanderia fulva) is a new invasive pest in the United States. At present, its management mainly relies on the use of synthetic insecticides, which are generally ineffective at producing lasting control of the pest, necessitating alternative environmentally friendly measures. In this study, we evaluated the feasibility of gene silencing to control this ant species. Six housekeeping genes encoding actin (NfActin), coatomer subunit β (NfCOPβ), arginine kinase (NfArgK), and V-type proton ATPase subunits A (NfvATPaseA), B (NfvATPaseB) and E (NfvATPaseE) were cloned...
May 22, 2018: Insect Science
https://www.readbyqxmd.com/read/29790084/cloning-and-expression-of-nlpa-gene-as-dna-vaccine-candidate-against-acinetobacter-baumannii
#7
Rassoul Hashemzehi, Abbas Doosti, Mohammad Kargar, Mojtaba Jaafarinia
Acinetobacter baumannii is one of the highly antibiotic-resistant bacteria that cause infections with high rate of death. This bacterium is one the common causes of infection worldwide leading to endemic and epidemic nosocomial infections. Despite many efforts, there is no effective vaccine against A. baumannii. As NlpA is one of the important antigenic factors in biogenesis of outer membrane vesicles, and OMV-based reported vaccines in A. baumannii stimulated the immune responses, this study was aimed to clone and express nlpA gene in eukaryotic HDF cells and evaluate the induced immunization following the administration of resulting construct as DNA vaccine in BALB/c mice...
May 22, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29789800/-lra-i-from-lactococcus-raffinolactis-bgtrk10-1-an-isoschizomer-of-eco-ri-exhibits-ion-concentration-dependent-specific-star-activity
#8
Marija Miljkovic, Milka Malesevic, Brankica Filipic, Goran Vukotic, Milan Kojic
Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses Lra I Type II restriction-modification enzyme, whose activity is similar to that shown for Eco RI; Lra I methyltransferase protects DNA from Eco RI cleavage. The gene encoding Lra I endonuclease was cloned and overexpressed in E. coli . Purified enzyme showed the highest specific activity at lower temperatures (between 13°C and 37°C) and was stable after storage at -20°C in 50% glycerol...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29789369/-wolbachia-w-stri-blocks-zika-virus-growth-at-two-independent-stages-of-viral-replication
#9
M J Schultz, A L Tan, C N Gray, S Isern, S F Michael, H M Frydman, J H Connor
Mosquito-transmitted viruses are spread globally and present a great risk to human health. Among the many approaches investigated to limit the diseases caused by these viruses are attempts to make mosquitos resistant to virus infection. Coinfection of mosquitos with the bacterium Wolbachia pipientis from supergroup A is a recent strategy employed to reduce the capacity for major vectors in the Aedes mosquito genus to transmit viruses, including dengue virus (DENV), Chikungunya virus (CHIKV), and Zika virus (ZIKV)...
May 22, 2018: MBio
https://www.readbyqxmd.com/read/29789357/reactivation-of-%C3%AE-globin-in-adult-%C3%AE-yac-mice-after-ex-vivo-and-in-vivo-hematopoietic-stem-cell-genome-editing
#10
Chang Li, Nikoletta Psatha, Pavel Sova, Sucheol Gil, Hongjie Wang, Jiho Kim, Chandana Kulkarni, Cristina Valensisi, R David Hawkins, George Stamatoyannopoulos, André Lieber
Disorders involving β-globin gene mutations, mainly β-thalassemia and sickle cell disease, represent a major target for hematopoietic stem/progenitor cell (HSPC) gene therapy. This includes CRISPR/Cas9-mediated genome editing approaches in adult CD34+ cells aimed toward the re-activation of fetal γ-globin expression in red blood cells. Because models involving erythroid differentiation of CD34+ cells have limitations in assessing γ-globin re-activation, we focused on human β-globin locus transgenic (β-YAC) mice...
May 22, 2018: Blood
https://www.readbyqxmd.com/read/29789158/rap1-promotes-proliferation-and-migration-of-vascular-smooth-muscle-cell-via-the-erk-pathway
#11
Qin Li, Yunfei Teng, Jian Wang, Miao Yu, Yiqing Li, Hong Zheng
BACKGROUND: Rap1 is involved in a multitude of cellular signal transduction pathways, which has extensively been linked to cell proliferation and migration. It has been shown to be important in the regulation of physiological and pathological processes. The present study aims to elucidate its detailed mechanistic in proliferation and migration. MATERIAL/METHODS: Vascular smooth muscle cells (VSMCs) were transfected with pcDNA3.1(empty vector), pcDNA3.1 containing Myc-Tagged-Rap1V12 (Rap1V12) or pcDNA3...
April 17, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29788739/identification-of-recurrent-risk-related-genes-and-establishment-of-support-vector-machine-prediction-model-for-gastric-cancer
#12
B Liu, J Tan, X Wang, X Liu
This study sought to investigate genes related to recurrent risk and establish a support vector machine (SVM) classifier for prediction of recurrent risk in gastric cancer (GC).Based on the gene expression profiling dataset GSE26253, feature genes that were significantly associated with survival time and status were screened out. Subsequently, protein-protein interaction (PPI) network was constructed for these feature genes, and genes in this network was optimized using betweenness centrality algorithm in order to identify genes potentially correlated with GC (named as GCGs)...
March 14, 2018: Neoplasma
https://www.readbyqxmd.com/read/29787571/rnai-screening-of-subtracted-transcriptomes-reveals-tumor-suppression-by-taurine-activated-gabaa-receptors-involved-in-volume-regulation
#13
Camiel L C Wielders, Pim van Nierop, Tinke L Vormer, Floris Foijer, Joanne Verheij, Johannes C Lodder, Jesper B Andersen, Huibert D Mansvelder, Hein Te Riele
To identify coding and non-coding suppressor genes of anchorage-independent proliferation by efficient loss-of-function screening, we have developed a method for enzymatic production of low complexity shRNA libraries from subtracted transcriptomes. We produced and screened two LEGO (Low-complexity by Enrichment for Genes shut Off) shRNA libraries that were enriched for shRNA vectors targeting coding and non-coding polyadenylated transcripts that were reduced in transformed Mouse Embryonic Fibroblasts (MEFs)...
2018: PloS One
https://www.readbyqxmd.com/read/29786752/overexpression-of-mir%C3%A2-17%C3%A2-5p-protects-against-high-glucose%C3%A2-induced-endothelial-cell-injury-by-targeting-e2f1%C3%A2-mediated-suppression-of-autophagy-and-promotion-of-apoptosis
#14
Yifeng Yuan, Xue Li, Maoquan Li
E2 promoter binding factor 1 (E2F1) has been reported to have an important regulatory role in cell survival during hyperglycemic conditions; however, the mechanisms remain to be fully elucidated. Bioinformatics analyses have suggested that microRNA (miR)‑17‑5p targets the 3'untranslated region (3'UTR) of E2F1. The aim of the present study was to characterize the protective effect of miR‑17‑5p/E2F1 on human umbilical vein endothelial cells (HUVECs) under high glucose (HG) conditions, to confirm the regulatory effect of miR‑17‑5p on E2F1/AMP‑activated protein kinase α2 (AMPKα2)‑mediated apoptosis and E2F1/mammalian target of rapamycin complex 1 (mTORC1)‑mediated autophagy...
May 21, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786113/overexpression-of-c%C3%A2-sis-inhibits-h2o2%C3%A2-induced-buffalo-rat-liver-cell-apoptosis-in-vitro-and-alleviates-liver-injury-in-a-rat-model-of-fulminant-hepatic-failure
#15
Hao Ding, Zhili Wen
The present study aimed to investigate the role of the C‑sis gene in the apoptosis of hepatocytes in vitro and in the liver function of a rat model of fulminant hepatic failure (FHF). Buffalo rat liver (BRL) cells were treated with hydrogen peroxide (H2O2) to induce apoptosis and then transfected with a C‑sis overexpression vector. A rat model of FHF was established, and C‑sis was overexpressed. The mRNA and protein expression of C‑sis were examined using reverse transcription‑polymerase chain reaction and western blot analyses, respectively...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786104/correlation-between-the-expression-of-il%C3%A2-18-and-deep-venous-thrombosis
#16
Guangdi Li, Rudan Zhou, Xueling Zhao, Riguang Liu, Chuan Ye
The present study aimed to investigate the effect of the expression of interleukin (IL)‑18 and related markers on deep venous thrombosis (DVT) to examine their correlation. Sprague‑Dawley rats of different models were established and were randomly assigned into three groups. The expression of IL‑18, nuclear factor (NF)‑κB and von Willebrand factor (vWF) were detected in blood samples. The inferior vena cava (IVC) was ligated to establish the DVT model. Rat IL‑18 overexpression and inhibition vectors were constructed...
May 16, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785047/rationally-designed-aav2-and-aavrh8r-capsids-provide-improved-transduction-in-the-retina-and-brain
#17
Jennifer A Sullivan, Lisa M Stanek, Michael J Lukason, Jie Bu, Shayla R Osmond, Elizabeth A Barry, Catherine R O'Riordan, Lamya S Shihabuddin, Seng H Cheng, Abraham Scaria
The successful application of adeno-associated virus (AAV) gene delivery vectors as a therapeutic paradigm will require efficient gene delivery to the appropriate cells in affected organs. In this study, we utilized a rational design approach to introduce modifications to the AAV2 and AAVrh8R capsids and the resulting variants were evaluated for transduction activity in the retina and brain. The modifications disrupted either capsid/receptor binding or altered capsid surface charge. Specifically, we mutated AAV2 amino acids R585A and R588A, which are required for binding to its receptor, heparan sulfate proteoglycans, to generate a variant referred to as AAV2-HBKO...
May 22, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29783989/high-co-expression-of-the-sdf1-cxcr4-axis-in-hepatocarcinoma-cells-is-regulated-by-annexina7-in-vitro-and-in-vivo
#18
Jingwen Wang, Yuhong Huang, Jun Zhang, Boyi Xing, Wei Xuan, Honghai Wang, He Huang, Jiayu Yang, Jianwu Tang
BACKGROUND: SDF1/CXCR4 and AnnexinA7 play important roles in many physiological and pathological conditions, but the molecular association between them in cancer cells has not been studied thus far. METHODS: The expression changes of SDF1/CXCR4 were detected by gene transcriptome sequencing, qRT-PCR, Western blotting, cytoimmunofluorescence and immunohistochemistry in mouse hepatocarcinoma F/P cells, AnnexinA7 downregulated expression F (FA7DOWN ) cells, AnnexinA7 overexpression P (PA7UP ) cells, AnnexinA7 unrelated sequence F (FSHUS ) cells, empty vector P (PNCEV ) cells and normal liver cells in vitro and in vivo...
May 21, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29782848/impaired-tfeb-mediated-lysosome-biogenesis-and-autophagy-promote-chronic-ethanol-induced-liver-injury-and-steatosis-in-mice
#19
Xiaojuan Chao, Shaogui Wang, Katrina Zhao, Yuan Li, Jessica A Williams, Tiangang Li, Hemantkumar Chavan, Partha Krishnamurthy, Xi C He, Linheng Li, Andrea Ballabio, Hong-Min Ni, Wen-Xing Ding
BACKGROUND & AIMS: Defects in lysosome function and autophagy contribute to pathogenesis of alcoholic liver disease. We investigated the mechanisms by which alcohol consumption affects these processes, evaluating the functions transcription factor EB (TFEB), which regulates lysosomal biogenesis. METHODS: We performed studies with GFP-LC3 mice, mice with liver-specific deletion of transcription factor EB (TFEB), mice with disruption of the transcription factor E3 gene (TFE3-knockout mice), mice with disruption of the Tefb and Tfe3 genes (TFEB, TFE3 double-knockout mice), and Tfebflox/flox albumin cre-negative mice (controls)...
May 18, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29782238/generation-of-a-lineage-ii-powassan-virus-deer-tick-virus-cdna-clone-assessment-of-flaviviral-genetic-determinants-of-tick-and-mosquito-vector-competence
#20
Joan L Kenney, Michael Anishchenko, Meghan Hermance, Hannah Romo, Ching-I Chen, Saravanan Thangamani, Aaron C Brault
The Flavivirus genus comprises a diverse group of viruses that utilize a wide range of vertebrate hosts and arthropod vectors. The genus includes viruses that are transmitted solely by mosquitoes or vertebrate hosts as well as viruses that alternate transmission between mosquitoes or ticks and vertebrates. Nevertheless, the viral genetic determinants that dictate these unique flaviviral host and vector specificities have been poorly characterized. In this report, a cDNA clone of a flavivirus that is transmitted between ticks and vertebrates (Powassan lineage II, deer tick virus [DTV]) was generated and chimeric viruses between the mosquito/vertebrate flavivirus, West Nile virus (WNV), were constructed...
May 21, 2018: Vector Borne and Zoonotic Diseases
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