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https://www.readbyqxmd.com/read/28222440/sumo-1-gene-silencing-inhibits-proliferation-and-promotes-apoptosis-of-human-gastric-cancer-sgc-7901-cells
#1
Lifang Jin, Kexin Shen, Tong Chen, Wei Yu, Huaiyu Zhang
BACKGROUND: It has been reported that blocking small ubiquitin-like modifier (SUMO) conjugation by silencing SUMO gene remarkably decreased tumor growth in vivo. However, few studies have examined the relationship between SUMO gene silencing and gastric cancer (GC). The study aims to explore the effects of SUMO-1 gene silencing on GC cell proliferation and apoptosis. METHODS: GC cells were cultured and divided into 5 groups: the blank group (without any transfection or treatment), the empty vector group (transfected with empty vector), the shRNA-SUMO-1-1 group (transfected with shRNA-SUMO-1-1 plasmid), the shRNA-SUMO-1-2 group (transfected with shRNA-SUMO-1-2 plasmid), and the shRNA-SUMO-1-3 group (transfected with shRNA-SUMO-1-3 plasmid)...
February 21, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28222438/microrna-29b-inhibits-angiogenesis-by-targeting-vegfa-through-the-mapk-erk-and-pi3k-akt-signaling-pathways-in-endometrial-carcinoma
#2
Hong-Xia Chen, Xiao-Xia Xu, Bu-Zhen Tan, Zhi Zhang, Xiao-Dong Zhou
OBJECTIVE: The purpose of this study is to explore the effects of microRNA-29b (miR-29b) regulating MAPK/ERK and PI3K/Akt signaling pathways on angiogenesis in endometrial carcinoma (EC) by targeting VEGFA. METHODS: Between February 2013 and April 2015, 126 EC patients admitted to the Second Affiliated Hospital of Nanchang University were randomly selected, with 126 EC tissues and the corresponding adjacent normal tissues collected after surgery. The human EC cell lines RL-95-2 and HEC-1-B and human endometrial cells were assigned to the normal group (human endometrial cells), the blank group (untransfected RL-95-2 or HEC-1-B cells), the pMIR-control group (RL-95-2 or HEC-1-B cells transfected with an empty vector), the pMIR-miR-29b group (RL-95-2 or HEC-1-B cells transfected with the miR-29b plasmid), LNA-control group (RL-95-2 or HEC-1-B cells transfected with an oligonucleotide inhibitors control), the LNA-miR-29b inhibitors group (RL-95-2 or HEC-1-B cells transfected with miRCURY LNATM miR-29b inhibitors), the LNA-miR-29b inhibitors + PD98059 group (RL-95-2 or HEC-1-B cells transfected with miRCURY LNATM miR-29b inhibitors and PD98059, an inhibitor of the MAPK/ERK signaling pathway) and the LNA-miR-29b inhibitors + wortmannin group (RL-95-2 or HEC-1-B cells transfected with miRCURY LNATM miR-29b inhibitors and wortmannin, an inhibitor of the PI3K/Akt signaling pathway)...
February 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28222350/a-thdreb-gene-from-tamarix-hispida-improved-the-salt-and-drought-tolerance-of-transgenic-tobacco-and-t-%C3%A2-hispida
#3
Guiyan Yang, Lili Yu, Kaimin Zhang, Yulin Zhao, Yucong Guo, Caiqiu Gao
Dehydration-responsive element-binding (DREB) transcription factors are important abiotic stress tolerance related genes, and some reports on the roles of DREB have primarily addressed herbal plants. To explore the abiotic stress tolerance role of DREB (ThDREB) from Tamarix hispida, a ThDREB gene with a complete ORF of 783 bp that encodes a 28.74 kDa protein with 260 amino acids, was isolated and functionally annotated. ThDREB expression was highly induced by NaCl, PEG, NaHCO3 and CdCl2 treatments, and the highest expression level (369...
February 10, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28222148/microrna-200a-silencing-protects-neural-stem-cells-against-cerebral-ischemia-reperfusion-injury
#4
Ji Ma, Shaofeng Shui, Xinwei Han, Dong Guo, Tengfei Li, Lei Yan
Neural stem cells (NSCs) play major roles in neurological recovery after cerebral infarction (CI). This study was trying to investigate whether miR-200a, a vital regulator in cell proliferation, migration and apoptosis, also has a role in oxygen-glucose deprivation/reperfusion (OGD/R) injured NSCs. In this study, primary NSCs were subjected to OGD/R conditions to mimic an in vitro CI model. Before OGD/R induction, NSCs were transfected with vector or shRNA against miR-200a to overexpress or suppress miR-200a expression...
2017: PloS One
https://www.readbyqxmd.com/read/28222026/hypoxic-regulation-of-the-expression-of-genes-encoded-estrogen-related-proteins-in-u87-glioma-cells-eff-ect-of-ire1-inhibition
#5
Minchenko Do, Riabovol Oo, Ratushna Oo, Minchenko Oh
OBJECTIVE: The aim of the present study was to examine the effect of inhibition of endoplasmic reticulum stress signaling, mediated by IRE1 (inositol requiring enzyme 1), which is a central mediator of the unfolded protein response on the expression of genes encoded estrogen related proteins (NRIP1/RIP140, TRIM16/EBBP, ESRRA/NR3B1, FAM162A/E2IG5, PGRMC2/PMBP, and SLC39A6/LIV-1) and their hypoxic regulation in U87 glioma cells for evaluation of their possible significance in the control of glioma cells proliferation...
January 1, 2017: Endocrine Regulations
https://www.readbyqxmd.com/read/28221337/modulation-of-digital-flexor-tendon-healing-by-vascular-endothelial-growth-factor-gene-transfection-in-a-chicken-model
#6
W F Mao, Y F Wu, Q Q Yang, Y L Zhou, J B Tang
A major challenge in tendon injury is the weak intrinsic healing capacity of tendon, which may cause re-rupture after surgery. Growth factors are believed to be critical during tendon healing. This study aimed to investigate the effects of vascular endothelial growth factor (VEGF) genes delivered by adeno-associated virus (AAV) vectors on tendon healing and molecular events involved in a chicken model. One hundred and twenty eight deep flexor tendons in the long toes of chickens were completely transected and injected with 2 × 10(9) particles of AAV2-VEGF or saline before surgically repaired...
February 21, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28221162/antiferromagnetic-spin-cantings-as-a-driving-force-of-ferroelectricity-in-multiferroic-cu-sub-2-sub-oseo-sub-3-sub
#7
Viacheslav Chizhikov, V Dmitrienko
Ferroelectric properties of cubic chiral magnet Cu<sub>2</sub>OSeO<sub>3</sub> can emerge due to the spin noncollinearity induced by antiferromagnetic cantings. The cantings are the result of the Dzyaloshinskii-Moriya interaction and in many ways similar to the ferromagnetic cantings in weak ferromagnets. An expression for the local electric polarization is derived, including terms with gradients of magnetization <b>M</b>(<b>r</b>). When averaged over the crystal the electric polarization has a non-vanishing part associated with the anisotropy of the crystal point group 23...
February 21, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28219833/statistical-appearance-models-based-on-probabilistic-correspondences
#8
Julia Krüger, Jan Ehrhardt, Heinz Handels
Model-based image analysis is indispensable in medical image processing. One key aspect of building statistical shape and appearance models is the determination of one-to-one correspondences in the training data set. At the same time, the identification of these correspondences is the most challenging part of such methods. In our earlier work, we developed an alternative method using correspondence probabilities instead of exact one-to-one correspondences for a statistical shape model (Hufnagel et al., 2008)...
February 7, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28219398/interaction-between-hcmv-pul83-and-human-aim2-disrupts-the-activation-of-the-aim2-inflammasome
#9
Yuan Huang, Di Ma, Heyu Huang, Yuanyuan Lu, Yi Liao, Lingling Liu, Xinglou Liu, Feng Fang
BACKGROUND: AIM2, a cytosolic DNA sensor, plays an important role during infection caused by pathogens with double-stranded DNA; however, its role in human cytomegalovirus (HCMV) infection remains unclear. Previously, we showed an increase in AIM2 protein levels during the early stage of HCMV infection and a decrease 24 h post infection. Because HCMV has developed a variety of strategies to evade host immunity, we speculated that this decline might be attributed to a viral immune escape mechanism...
February 20, 2017: Virology Journal
https://www.readbyqxmd.com/read/28218919/targeting-casp8-and-fadd-like-apoptosis-regulator-ameliorates-nonalcoholic-steatohepatitis-in-mice-and-nonhuman-primates
#10
Pi-Xiao Wang, Yan-Xiao Ji, Xiao-Jing Zhang, Ling-Ping Zhao, Zhen-Zhen Yan, Peng Zhang, Li-Jun Shen, Xia Yang, Jing Fang, Song Tian, Xue-Yong Zhu, Jun Gong, Xin Zhang, Qiao-Fang Wei, Yong Wang, Jing Li, Lu Wan, Qingguo Xie, Zhi-Gang She, Zhihua Wang, Zan Huang, Hongliang Li
Nonalcoholic steatohepatitis (NASH) is a progressive disease that is often accompanied by metabolic syndrome and poses a high risk of severe liver damage. However, no effective pharmacological treatment is currently available for NASH. Here we report that CASP8 and FADD-like apoptosis regulator (CFLAR) is a key suppressor of steatohepatitis and its metabolic disorders. We provide mechanistic evidence that CFLAR directly targets the kinase MAP3K5 (also known as ASK1) and interrupts its N-terminus-mediated dimerization, thereby blocking signaling involving ASK1 and the kinase MAPK8 (also known as JNK1)...
February 20, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28218744/live-cell-imaging-to-compare-the-transfection-and-gene-silencing-efficiency-of-calcium-phosphate-nanoparticles-and-a-liposomal-transfection-agent
#11
S Chernousova, M Epple
The processing of DNA (for transfection) and siRNA (for gene silencing), introduced into HeLa cells by triple-shell calcium phosphate nanoparticles, was followed by live-cell imaging. For comparison, the commercial liposomal transfection agent Lipofectamine(TM) was used. The cells were incubated with these delivery systems, carrying either eGFP-encoding DNA or siRNA against eGFP. In the latter case, HeLa cells which stably expressed eGFP were used. The expression of eGFP started after 5 h in the case of nanoparticles and after 4 h in the case of Lipofectamine...
February 20, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28218713/transgene-expression-and-host-cell-responses-to-replication-defective-single-cycle-and-replication-competent-adenovirus-vectors
#12
Catherine M Crosby, Michael A Barry
Most adenovirus (Ad) vectors are E1 gene deleted replication defective (RD-Ad) vectors that deliver one transgene to the cell and all expression is based on that one gene. In contrast, E1-intact replication-competent Ad (RC-Ad) vectors replicate their DNA and their transgenes up to 10,000-fold, amplifying transgene expression markedly higher than RD-Ad vectors. While RC-Ad are more potent, they run the real risk of causing adenovirus infections in vector recipients and those that administer them. To gain the benefits of transgene amplification, but avoid the risk of Ad infections, we developed "single cycle" Ad (SC-Ad) vectors...
February 18, 2017: Genes
https://www.readbyqxmd.com/read/28218496/functional-analysis-of-bioactivated-surfaces
#13
Selgai Haidari, Marko Boskov, Ulrike Schillinger, Klaus-Dietrich Wolff, Christian Plank, Andreas Kolk
Common scaffold surfaces, such as titanium, can have side effects, e.g., infections, cytotoxicity, impaired osseointegration or low regeneration rates for bone tissue. These effects lead to poor implant integration or even implant loss. Therefore, bioactive implants are promising instruments in tissue regeneration. Osteoinductive elements, such as growth factors and anti-infectives, support wound healing and bone growth and thereby enable faster osseointegration, even in elderly patients. In this study, titanium surfaces were coated with a poly-(D,L-lactide) (PDLLA) layer containing different concentrations of copolymer-protected gene vectors (COPROGs) to locally provide bone morphogenetic protein-2 (BMP-2) or activated anti-infective agents, such as chlorhexidine gluconate, triclosan and metronidazole, to prevent peri-implantitis...
February 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28215880/adenovirus-vector-mediated-ex-vivo-gene-transfer-of-brain-derived-neurotrophic-factor-bdnf-tohuman-umbilical-cord-blood-derived-mesenchymal-stem-cells-ucb-mscs-promotescrush-injured-rat-sciatic-nerve-regeneration
#14
Wei-Hong Hei, Akram A Almansoori, Mi-Ae Sung, Kyung-Won Ju, Nari Seo, Sung-Ho Lee, Bong-Ju Kim, Soung-Min Kim, Jeong Won Jahng, Hong He, Jong-Ho Lee
This study was designed toinvestigate the efficacy of adenovirus vector-mediated brain-derived neurotrophic factor (BDNF)ex vivo gene transfer to human umbilical cord blood-derived mesenchymal stem cells(UCB-MSCs) in a rat sciatic nerve crush injury model. BDNF protein and mRNA expression after infection was checked through an enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). Male Sprague-Dawley rats (200-250g, 6 weeks old) were distributed into threegroups (n=20 each): the control group, UCB-MSC group, and BDNF-adenovirus infected UCB-MSC (BDNF-Ad+UCB-MSC) group...
February 12, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28215840/cytidine-deaminase-apobec3a-induction-in-fallopian-epithelium-after-exposure-to-follicular-fluid
#15
Pavla Brachova, Nehemiah S Alvarez, Bradley J Van Voorhis, Lane K Christenson
OBJECTIVE: Ovarian carcinomas that originate from fallopian epithelial cells are suggested to arise due to repeated exposure to ovulatory follicular fluid (FF). Mechanistic explanation(s) for how this occurs are unknown. Here, we sought to understand if FF exposure to fallopian epithelial cells could induce DNA damage and expression of a known family of DNA mutators, apolipoprotein B mRNA editing enzyme, catalytic polypeptide (APOBEC) cytidine deaminases. METHODS: Follicular fluid and matched patient plasma samples were obtained from donors...
February 16, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28215614/genome-characterization-infectivity-assays-of-in-vitro-and-in-vivo-infectious-transcripts-of-soybean-yellow-mottle-mosaic-virus-from-india-reveals-a-novel-short-mild-genotype
#16
Nagamani Sandra, Abdul Kader Jailani, Rakesh Kumar Jain, Bikash Mandal
Nucleotide sequence of a distinct soybean yellow mottle mosaic virusisolate from Vignaradiata (mungbean isolate, SYMMV-Mb) from India was determined and compared with othermembers of the family Tombusviridae. The complete monopartite single-stranded RNA genome of SYMMV-Mb consisted of 3974nt with six putative open reading frames and includes 5' and 3' untranslated regions of 35 and 254nt, respectively. SYMMV-Mb genome shared 75% nt sequence identity at complete genome level and 67-92% identity at all ORFs level with SYMMV Korean and USA isolates (soybean isolates) followed by CPMoV, whereas it shared very low identity with other tombusviridae members (5 to 41%)...
February 16, 2017: Virus Research
https://www.readbyqxmd.com/read/28215587/surface-expression-of-helicobacter-pylori-hpaa-adhesion-antigen-on-vibrio-cholerae-enhanced-by-co-expressed-enterotoxigenic-escherichia-coli-fimbrial-antigens
#17
Joshua Tobias, Michael Lebens, Sun Nyunt Wai, Jan Holmgren, Ann-Mari Svennerholm
Helicobacter pylori infection can cause peptic ulceration and is associated with gastric adenocarcinoma. This study aimed to construct and characterize a non-virulent Vibrio cholerae O1 strain, which grows more rapidly than H. pylori, as vector for H. pylori antigens for possible use as a vaccine strain against H. pylori. This was done by recombinant expression of the H. pylori adhesion antigen HpaA alone or, as a proof of principle, together with different colonization factor (CF) antigens of enterotoxigenic Escherichia coli (ETEC) which may enhance immune responses against HpaA...
February 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28214886/adenovirus-vector-harboring-the-hbcag-and-tripeptidyl-peptidase-ii-genes-induces-potent-cellular-immune-responses-in-vivo
#18
Quanhui Tan, Siyuan Ma, Jianjun Hu, Xiaohua Chen, Yongsheng Yu, Zhenghao Tang, Guoqin Zang
BACKGROUND: Chronic hepatitis B virus (HBV) infection is associated with a weak but specific cellular immune response of the host to HBV. Tripeptidyl peptidaseⅡ (TPPⅡ), an intracellular macromolecule and proteolytic enzyme, plays an important complementary and compensatory role for the proteasome during viral protein degradation and major histocompatibility complex class I antigen presentation by inducing a specific cellular immune response in vivo. Based on a previous study, we aimed to explore the role of MHC class I antigen presentation in vivo and the mechanisms that may be involved...
January 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214873/nel-like-molecule-1-nell1-is-regulated-by-bone-morphogenetic-protein-9-bmp9-and-potentiates-bmp9-induced-osteogenic-differentiation-at-the-expense-of-adipogenesis-in-mesenchymal-stem-cells
#19
Jing Wang, Junyi Liao, Fugui Zhang, Dongzhe Song, Minpeng Lu, Jianxiang Liu, Qiang Wei, Shengli Tang, Hao Liu, Jiaming Fan, Yulong Zou, Dan Guo, Jiayi Huang, Feng Liu, Chao Ma, Xue Hu, Li Li, Xiangyang Qu, Liqun Chen, Yaguang Weng, Michael J Lee, Tong-Chuan He, Russell R Reid, Jiye Zhang
BACKGROUND: BMP9 induces both osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs). Nell1 is a secretory glycoprotein with osteoinductive and anti-adipogenic activities. We investigated the role of Nell1 in BMP9-induced osteogenesis and adipogenesis in MSCs. METHODS: Previously characterized MSCs iMEFs were used. Overexpression of BMP9 and NELL1 or silencing of mouse Nell1 was mediated by adenoviral vectors. Early and late osteogenic and adipogenic markers were assessed by staining techniques and qPCR analysis...
January 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214835/up-regulation-of-tgf-%C3%AE-promotes-tendon-to-bone-healing-after-anterior-cruciate-ligament-reconstruction-using-bone-marrow-derived-mesenchymal-stem-cells-through-the-tgf-%C3%AE-mapk-signaling-pathway-in-a-new-zealand-white-rabbit-model
#20
Rui Wang, Bin Xu, Hong-Gang Xu
BACKGROUND/AIMS: This study aimed to explore the role of TGF-β in tendon-to-bone healing after anterior cruciate ligament (ACL) reconstruction using bone marrow-derived mesenchymal stem cells (BMSCs) through the TGF-β/MAPK signaling pathway in a New Zealand white rabbit model. METHODS: A total of 72 healthy male New Zealand white rabbits were selected for these experiments. Flow cytometry and immunofluorescence were used to detect the expression of BMSC surface markers, and qRT-PCR was performed to detect TGF-β mRNA expression...
January 20, 2017: Cellular Physiology and Biochemistry
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