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Stem cek

Mei Shang, Baofeng Su, Dayan A Perera, Ahmed Alsaqufi, Elizabeth A Lipke, Sehriban Cek, David A Dunn, Zhenkui Qin, Eric Peatman, Rex A Dunham
Our aim was to transplant blue catfish germ line stem cells into blastulae of triploid channel catfish embryos to produce interspecific xenogenic catfish. The morphological structure of the gonads of blue catfish (Ictalurus furcatus) in ~ 90- to 100-day-old juveniles, two-year-old juveniles, and mature adults was studied histologically. Both oogonia (12-15 μm, diameter with distinct nucleus 7-8 μm diameter) and spermatogonia (12-15 μm, with distinct nucleus 6-7.5 μm diameter) were found in all ages of fish...
April 2018: Fish Physiology and Biochemistry
Mei Shang, Baofeng Su, Elizabeth A Lipke, Dayan A Perera, Chao Li, Zhenkui Qin, Yun Li, David A Dunn, Sehriban Cek, Eric Peatman, Rex A Dunham
Testicular germ cells of channel catfish, Ictalurus punctatus, and blue catfish, I. furcatus were separated into four layers with Percoll density gradient centrifugation, containing different cell types (40% in the first layer were spermatogonial stem cells, SSCs). Expression of seventeen genes was analyzed for cells from different layers by real-time quantitative PCR. Pfkfb4, Urod, Plzf, Integrin6, IntegrinV, Thy1 and Cdh1 genes showed the same expression change pattern in both channel and blue catfish as these genes were down-regulated in the spermatocytes and even more so in spermatids...
December 2015: Fish Physiology and Biochemistry
Rakesh Rachamadugu, Jeong Yoon Lee, Ann Wooming, Byung-Whi Kong
MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that play a pivotal role in the regulation of gene expression at the post transcriptional level. Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus belonging to the herpesviridae family. It causes an acute respiratory disorder in chicken resulting in high mortality rates. ILTV encoding miRNAs have been identified in cell cultures infected by ILTV. Seven candidates ILTV encoding miRNAs were identified by the 454 FLX genome sequencing method...
December 2009: Virus Genes
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